Sharma S, Muthu S, Jeyaraman M, Ranjan R, Jha SK. Translational products of adipose tissue-derived mesenchymal stem cells: Bench to bedside applications. World J Stem Cells 2021; 13(10): 1360-1381 [PMID: 34786149 DOI: 10.4252/wjsc.v13.i10.1360]
Reader's ID:
03550310
Submitted on:
November 04, 2021, 19:11
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Reader Comments:
This review about the different adipose derived MSCs products is very well organized and easy to follow, written in a clear language and rationale sequence.
Authors discused the adipose tissue derived products and breifly reviewed some scientific facts and some of the studies conducted with their conclusions accuratly.
Only one part was not clearly written in this manuscript regarding the storage of ADSCs , where authors stated that ADSCs can be stored at -80C in liquid nitrogen for up to 6 months in page 1364. Liquid nitrogen is known to have 2 phases a -132 C gas phase and a -196 C liquid phase. Cells when cryopreserved they stay in -80 over night in a tempreture regulator to slowly freeze the cells then tranfered to liguid nirogen (gas or liquid phase) for storage for years. keeping them at -80 would affect the cell viability when thawed.
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