|
1
|
Vázquez E, de Gregorio Ó, Soriano V, Álvarez C, Ortega-de la Puente A, de la Cruz-Echeandía M, Blanco-Valencia XP, Royuela A, Esteban-Sampedro J, Martín-Portugués M, Corral O, Moreno-Torres V. Pregnancy-related listeriosis in Spain. J Infect Public Health 2025; 18:102706. [PMID: 40014937 DOI: 10.1016/j.jiph.2025.102706] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/23/2024] [Revised: 02/11/2025] [Accepted: 02/13/2025] [Indexed: 03/01/2025] Open
Abstract
BACKGROUND Pregnant women are at high risk of acquiring listeriosis, resulting in severe fetal and neonatal outcomes. METHODS All hospitalizations with a listeriosis diagnosis in pregnant women (obstetric listeriosis) and/or newborns (neonatal listeriosis) in Spain from 2000 to 2021 were examined using the National Registry of Hospital Discharges, employing ICD-9 and -10 coding lists. RESULTS A total of 540 and 450 hospital admissions for obstetric listeriosis and neonatal listeriosis were identified, respectively, with 146 adverse fetal-neonatal outcomes (miscarriage, fetal loss, stillbirth, and neonatal death). The incidence of obstetric listeriosis, neonatal listeriosis, and adverse fetal-neonatal outcomes (5.7, 4.7, and 1.5 per 100,000 deliveries, respectively) rose significantly from 2000 to 2021. No maternal deaths were recorded among women hospitalized with obstetric listeriosis. However, 9.8 % experienced miscarriage, related to bacteremia (OR=2.46), 6.3 % fetal loss and 5.9 % stillbirths, associated with chorioamnionitis (OR=3.42), which was identified in 77.7 % of 254 deliveries. Overall, 51.1 % of newborns developed sepsis, 58.9 % prematurity, 26.9 % ARDS, and 9.8 % died. ARDS (OR=2.76) and prematurity (OR=5.07) were associated with perinatal death in newborns with listeriosis. Pregnancy-related listeriosis was associated with increased risks of miscarriage (OR=1.75), intrauterine death (OR=17), preterm labor (OR=8.78), fetal distress (OR=2.10), cesarean section (OR=1.68), and stillbirth (OR=23.57). CONCLUSIONS Admissions for obstetric listeriosis and neonatal listeriosis in Spain have risen significantly from 2000 to 2021. Pregnancy-related listeriosis has a deleterious impact on fetal and neonatal outcomes, including miscarriages, fetal loss, stillbirth, and neonatal death. Surveillance, prevention, and prompt management of pregnant women with listeriosis and newborns with neonatal infection are warranted.
Collapse
Affiliation(s)
- Elena Vázquez
- UNIR Health Sciences School and Medical Center, Universidad Internacional de La Rioja, Madrid, Spain
| | - Óscar de Gregorio
- Instituto de Transferencia e Investigación (ITEI), Universidad Internacional de La Rioja, Madrid, Spain
| | - Vicente Soriano
- UNIR Health Sciences School and Medical Center, Universidad Internacional de La Rioja, Madrid, Spain
| | - Carmen Álvarez
- UNIR Health Sciences School and Medical Center, Universidad Internacional de La Rioja, Madrid, Spain
| | | | | | | | - Ana Royuela
- Biostatistics Unit, Instituto de Investigación Puerta de Hierro-Segovia de Arana, Madrid, Spain; Center for Biomedical Research in Epidemiology and Public Health Network (CIBERESP), Spain
| | - Jorge Esteban-Sampedro
- Internal Medicine Department, Health Research Institute Puerta de Hierro-Segovia de Arana (IDIPHIM), Hospital Universitario Puerta de Hierro, Majadahonda, Madrid, Spain
| | - Mario Martín-Portugués
- Internal Medicine Department, Health Research Institute Puerta de Hierro-Segovia de Arana (IDIPHIM), Hospital Universitario Puerta de Hierro, Majadahonda, Madrid, Spain
| | - Octavio Corral
- UNIR Health Sciences School and Medical Center, Universidad Internacional de La Rioja, Madrid, Spain
| | - Víctor Moreno-Torres
- UNIR Health Sciences School and Medical Center, Universidad Internacional de La Rioja, Madrid, Spain; Internal Medicine Department, Health Research Institute Puerta de Hierro-Segovia de Arana (IDIPHIM), Hospital Universitario Puerta de Hierro, Majadahonda, Madrid, Spain.
| |
Collapse
|
|
2
|
Balogh DC, Kovács K, Kovács ŐZ, Regős E, Fintha A, Harmath Á, Szabó M, Gasparics Á, Varga P. Association of Meconium-Stained Amniotic Fluid and Histological Chorioamnionitis with Fetal Inflammatory Response in Preterm Deliveries. CHILDREN (BASEL, SWITZERLAND) 2025; 12:477. [PMID: 40310124 PMCID: PMC12025836 DOI: 10.3390/children12040477] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 02/10/2025] [Revised: 04/02/2025] [Accepted: 04/02/2025] [Indexed: 05/02/2025]
Abstract
BACKGROUND The importance and etiology of meconium-stained amniotic fluid (MSAF) in preterm pregnancies are still poorly understood. Among other factors, intrauterine inflammation is proposed to be a pathophysiological change associated with MSAF. To study the extent of intrauterine inflammation, histological evaluation represents the "gold standard" of diagnostics. OBJECTIVES To investigate the concomitant occurrence of MSAF and histological chorioamnionitis (HCA) and fetal inflammatory response (FIR). To investigate the incidence of short-term neonatal outcomes in preterm infants born from MSAF. MATERIALS AND METHODS We conducted a single-center retrospective study in a tertiary neonatal intensive care unit between 2020 and 2022. 237 preterm infants born ≤ 32 weeks or with ≤1500 g birthweight were investigated. The group of infants born from MSAF was compared to the group of infants born from clear amniotic fluid (CAF). The variables measured were the following: HCA, FIR, maternal and fetal vascular malformations (MVM, FVM), maternal clinical and laboratory signs of chorioamnionitis (CA), early neonatal outcomes, neonatal white blood cell count (WBC) in the first day of life, and neonatal c-reactive protein (CRP) level on the second day of life. Histological evaluation of the placenta and the umbilical cord was based on the recommendation of the 2014 Amsterdam Placental Workshop Group Consensus Statement (APWGCS). RESULTS Out of 237 preterm infants (mean gestational age: 28.6 (95% CI: 28.2; 28.9) weeks, mean birth weight: 1165 (95% CI: 1110; 1218) grams), 22 were born from MSAF. There was no difference between the perinatal characteristics of the two groups. A higher incidence of HCA (54.5% vs. 32.6%; p: <0.001), a higher incidence of stage 3 HCA (45.4% vs. 9.3%), a higher incidence of FIR (50% vs. 16.7%; p: <0.001), and a higher incidence of stage 3 FIR (18.2% vs. 1.9%) were found in the MSAF group in comparison with the CAF group. A higher incidence of elevated (>30 mg/L) maternal CRP level (36.8% vs. 15.3%; p: 0.02) and elevated (>15 mg/L) neonatal CRP level (31.8% vs. 14.4%; p: 0.03) was detected in the MSAF group. Among neonatal complications, severe (Stage III/IV) intraventricular hemorrhage (IVH) had a higher incidence in the MSAF group (22.2% vs. 5.1%; p: 0.005). CONCLUSION MSAF in preterm pregnancies is associated with a severe maternal and fetal inflammatory response in the placenta and the umbilical cord. MSAF is also accompanied by elevated systemic inflammatory parameters and a higher incidence of severe neonatal IVH as well.
Collapse
Affiliation(s)
- Dóra Csenge Balogh
- Department of Obstetrics and Gynecology, Semmelweis University, H-1088 Budapest, Hungary; (D.C.B.); (K.K.); (Ő.Z.K.); (Á.H.); (Á.G.)
| | - Kinga Kovács
- Department of Obstetrics and Gynecology, Semmelweis University, H-1088 Budapest, Hungary; (D.C.B.); (K.K.); (Ő.Z.K.); (Á.H.); (Á.G.)
| | - Őzike Zsuzsanna Kovács
- Department of Obstetrics and Gynecology, Semmelweis University, H-1088 Budapest, Hungary; (D.C.B.); (K.K.); (Ő.Z.K.); (Á.H.); (Á.G.)
| | - Eszter Regős
- Department of Pathology and Experimental Cancer Research, Semmelweis University, H-1088 Budapest, Hungary; (E.R.); (A.F.)
| | - Attila Fintha
- Department of Pathology and Experimental Cancer Research, Semmelweis University, H-1088 Budapest, Hungary; (E.R.); (A.F.)
| | - Ágnes Harmath
- Department of Obstetrics and Gynecology, Semmelweis University, H-1088 Budapest, Hungary; (D.C.B.); (K.K.); (Ő.Z.K.); (Á.H.); (Á.G.)
| | - Miklós Szabó
- Department of Neonatology, Pediatric Center Semmelweis University, 53-54 Bokay Janos St 1083, H-1088 Budapest, Hungary;
| | - Ákos Gasparics
- Department of Obstetrics and Gynecology, Semmelweis University, H-1088 Budapest, Hungary; (D.C.B.); (K.K.); (Ő.Z.K.); (Á.H.); (Á.G.)
| | - Péter Varga
- Department of Obstetrics and Gynecology, Semmelweis University, H-1088 Budapest, Hungary; (D.C.B.); (K.K.); (Ő.Z.K.); (Á.H.); (Á.G.)
| |
Collapse
|
|
3
|
Ahmed S, Ba Khamis F, Hazari K, Harb DK, Abdelkareem W, Alhubaishi L. Listeriosis Infection in Pregnancy: A Case Series. Cureus 2024; 16:e74135. [PMID: 39712836 PMCID: PMC11663029 DOI: 10.7759/cureus.74135] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 11/20/2024] [Indexed: 12/24/2024] Open
Abstract
Listeriosis is a rare foodborne febrile illness that has a unique predilection for the pregnant population. Listeriosis during pregnancy can cross the placenta, resulting in vertical transmission to the fetus which frequently results in adverse pregnancy outcomes. This is a case series on three pregnant patients who were treated for listeriosis at Latifa Women and Children's Hospital. All three patients reported in this series had no medical comorbidities or pregnancy risk factors; they presented with fever and abdominal and/or back pain; and of significance, none of the patients reported a history of gastrointestinal tract symptoms. Two cases were in the second trimester and one in the third trimester. All cases resulted in fetal loss with 100% fetal mortality. There was no maternal mortality or long-term morbidity in any of the patients.
Collapse
Affiliation(s)
- Salwa Ahmed
- Obstetrics and Gynecology, Dubai Health, Dubai, ARE
| | | | - Komal Hazari
- Internal Medicine, Latifa Women and Children's Hospital, Dubai, ARE
| | - Deemah K Harb
- Internal Medicine, Latifa Women and Children's Hospital, Dubai, ARE
| | | | - Laila Alhubaishi
- Obstetrics and Gynecology, Latifa Women and Children's Hospital, Dubai, ARE
| |
Collapse
|
|
4
|
Roero S, Peila C, Arduino S, Deantoni S, Coscia A, Revelli A. Lysteria Monocytogenes Infection during Monochorionic Twin Pregnancy: Case Report and Review of the Literature. J Clin Med 2024; 13:6061. [PMID: 39458011 PMCID: PMC11508886 DOI: 10.3390/jcm13206061] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/04/2024] [Revised: 10/07/2024] [Accepted: 10/10/2024] [Indexed: 10/28/2024] Open
Abstract
Listeriosis is a rare but severe foodborne disease caused by Listeria Monocytogenes (LM), a small facultative intracellular bacillus. When occurring in pregnant women, it can be vertically transmitted to the fetus and the newborn. Infected women usually display aspecific and mild symptoms, and rarely develop the severe forms of the disease (such as neurolisteriosis). On the contrary, fetal and neonatal listeriosis can lead to complications such as fetal loss, preterm birth, neonatal sepsis, and respiratory distress syndrome (RDS). Prompt diagnosis is one of the main challenges because of the aspecific presentation of the disease; therapy relies on antibiotics that reach high intracellular concentration and can penetrate and pass the placenta reaching the fetus. Herein we report an infrequent case of LM infection involving a woman with monochorionic diamniotic twin pregnancy, followed by a comprehensive review of the available literature on listeriosis in pregnancy.
Collapse
Affiliation(s)
- Sofia Roero
- Twin Pregnancy Unit, Gynecology and Obstetrics 2U, A.O.U. Città della Salute e della Scienza, Sant’Anna Hospital, University of Turin, 10126 Turin, Italy
| | - Chiara Peila
- Neonatal Unit, Department of Public Health and Pediatrics, University of Turin, 10126 Turin, Italy
| | - Silvana Arduino
- Twin Pregnancy Unit, Gynecology and Obstetrics 2U, A.O.U. Città della Salute e della Scienza, Sant’Anna Hospital, University of Turin, 10126 Turin, Italy
| | - Sonia Deantoni
- Neonatal Unit, Department of Public Health and Pediatrics, University of Turin, 10126 Turin, Italy
| | - Alessandra Coscia
- Neonatal Unit, Department of Public Health and Pediatrics, University of Turin, 10126 Turin, Italy
| | - Alberto Revelli
- Twin Pregnancy Unit, Gynecology and Obstetrics 2U, A.O.U. Città della Salute e della Scienza, Sant’Anna Hospital, University of Turin, 10126 Turin, Italy
| |
Collapse
|
|
5
|
D'Sa S, Saleh E, Chaudhary S, Rodriguez M. Post-infectious hydrocephalus complicating Listeria meningitis in a healthy newborn. BMJ Case Rep 2024; 17:e258825. [PMID: 38914530 DOI: 10.1136/bcr-2023-258825] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 06/26/2024] Open
Abstract
Listeria monocytogenes is a relatively uncommon cause of foodborne infection in the general population. Most cases of Listeriosis occur among newborns, pregnant women, the elderly and those with impairment of cellular immunity. Neonatal Listeria meningitis is rare. We present a case of Listeria meningitis at the age of 15 days in a previously healthy neonate who presented with acute onset of fever, poor feeding and lethargy. Sepsis workup revealed L. monocytogenes identified in cerebrospinal fluid PCR and culture. The infant's course was complicated by transient syndrome of inappropriate antidiuretic hormone and subsequent hydrocephalus that required a ventriculoperitoneal shunt placement. Though rare, neonatal infections due to Listeria can present with meningitis leading to serious and devastating complications. Our case emphasises the importance of considering Listeria in cases of neonatal meningitis and the value of close follow-up of such cases through early detection and management of acute and long-term complications.
Collapse
Affiliation(s)
- Saskia D'Sa
- Pediatrics, Southern Illinois University School of Medicine, Springfield, Illinois, USA
| | - Ezzeldin Saleh
- Pediatrics-Infectious Diseases, Southern Illinois University School of Medicine, Springfield, Illinois, USA
| | - Subhash Chaudhary
- Pediatrics-Infectious Diseases, Southern Illinois University School of Medicine, Springfield, Illinois, USA
| | - Marcela Rodriguez
- Pediatrics-Infectious Diseases, Southern Illinois University School of Medicine, Springfield, Illinois, USA
| |
Collapse
|
|
6
|
Hernandez Lopez AL, Fassett MJ. Infectious diseases in pregnancy: A continuing struggle. Case Rep Womens Health 2024; 42:e00610. [PMID: 39021443 PMCID: PMC11252523 DOI: 10.1016/j.crwh.2024.e00610] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/11/2024] [Accepted: 04/11/2024] [Indexed: 07/20/2024] Open
Affiliation(s)
- Adrian L. Hernandez Lopez
- Department of Obstetrics and Gynecology, University of California, San Francisco Mission Bay Medical Center, 1855 4 Street, San Francisco, CA 94158, United States of America
| | - Michael J. Fassett
- Department of Obstetrics and Gynecology, Kaiser Permanente Southern California, West Los Angeles Medical Center, 6041 Cadillac Ave 3rd Floor, Los Angeles, CA 90034, United States of America
| |
Collapse
|
|
7
|
Hoo R, Ruiz-Morales ER, Kelava I, Rawat M, Mazzeo CI, Tuck E, Sancho-Serra C, Chelaghma S, Predeus AV, Murray S, Fernandez-Antoran D, Waller RF, Álvarez-Errico D, Lee MCS, Vento-Tormo R. Acute response to pathogens in the early human placenta at single-cell resolution. Cell Syst 2024; 15:425-444.e9. [PMID: 38703772 DOI: 10.1016/j.cels.2024.04.002] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/19/2023] [Revised: 12/01/2023] [Accepted: 04/16/2024] [Indexed: 05/06/2024]
Abstract
The placenta is a selective maternal-fetal barrier that provides nourishment and protection from infections. However, certain pathogens can attach to and even cross the placenta, causing pregnancy complications with potential lifelong impacts on the child's health. Here, we profiled at the single-cell level the placental responses to three pathogens associated with intrauterine complications-Plasmodium falciparum, Listeria monocytogenes, and Toxoplasma gondii. We found that upon exposure to the pathogens, all placental lineages trigger inflammatory responses that may compromise placental function. Additionally, we characterized the responses of fetal macrophages known as Hofbauer cells (HBCs) to each pathogen and propose that they are the probable niche for T. gondii. Finally, we revealed how P. falciparum adapts to the placental microenvironment by modulating protein export into the host erythrocyte and nutrient uptake pathways. Altogether, we have defined the cellular networks and signaling pathways mediating acute placental inflammatory responses that could contribute to pregnancy complications.
Collapse
Affiliation(s)
- Regina Hoo
- Wellcome Sanger Institute, Cambridge, UK; Centre for Trophoblast Research, University of Cambridge, Cambridge, UK
| | | | - Iva Kelava
- Wellcome Sanger Institute, Cambridge, UK
| | - Mukul Rawat
- Wellcome Sanger Institute, Cambridge, UK; Wellcome Centre for Anti-Infectives Research, Division of Biological Chemistry and Drug Discovery, University of Dundee, Dundee, UK
| | | | | | | | - Sara Chelaghma
- Department of Biochemistry, University of Cambridge, Cambridge, UK
| | | | | | - David Fernandez-Antoran
- Wellcome/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK; Department of Pathology, University of Cambridge, Cambridge, UK
| | - Ross F Waller
- Department of Biochemistry, University of Cambridge, Cambridge, UK
| | | | - Marcus C S Lee
- Wellcome Sanger Institute, Cambridge, UK; Wellcome Centre for Anti-Infectives Research, Division of Biological Chemistry and Drug Discovery, University of Dundee, Dundee, UK.
| | - Roser Vento-Tormo
- Wellcome Sanger Institute, Cambridge, UK; Centre for Trophoblast Research, University of Cambridge, Cambridge, UK.
| |
Collapse
|
|
8
|
Li D, Duan L, Zhang X, Cao J. Clinical significance of rapid detection and diagnosis of Listeria infection in blood with mass spectrometry. J Obstet Gynaecol Res 2024; 50:508-515. [PMID: 38087434 DOI: 10.1111/jog.15859] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/12/2023] [Accepted: 11/30/2023] [Indexed: 03/04/2024]
Abstract
OBJECTIVE Listeriosis is caused by the bacterium, Listeria monocytogenes, and is a significant health concern because of high hospitalization and mortality rates. This study reports seven cases of pregnancy-associated listeriosis diagnosed with matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry performed on infection-positive blood culture. METHODS Blood culture-positive samples of seven patients whose pregnancy was complicated by Listeria infection and treated at Xuanwu Hospital of Capital Medical University between January 2016 and December 2021 were analyzed retrospectively. Strains identified by MALDI-TOF mass spectrometry were compared with colony identification results. Identification accuracy and consistency were assessed. RESULTS A total of seven strains of Listeria were collected from seven pregnant women presented with fever (37.6-39.9°C). Clinical abnormalities included abnormal liver function, emaciation, hypoalbuminemia, hypocalcemia, hypokalemia, hyponatremia, ketosis, mild to moderate anemia, leukopenia, and thrombocytopenia. Compared with the traditional culture method, MALDI-TOF mass spectrometry led to much earlier identification (4-6 h vs. 3-4 days) with 100% identification accuracy. Of the seven pregnancies complicated by Listeria, only two led to live births. Of the five fetal deaths, three occurred in the second trimester. CONCLUSION In this series of pregnancy-associated listeriosis cases, the fetal mortality rate was 71%. MALDI-TOF mass spectrometry is a valuable method that can identify Listeria from blood culture rapidly and accurately.
Collapse
Affiliation(s)
- Dan Li
- Department of Obstetrics and Gynecology, Xuanwu Hospital of Capital Medical University, Beijing, China
| | - Linyan Duan
- Education Section, Xuanwu Hospital of Capital Medical University, Beijing, China
| | - Xiaoyan Zhang
- Education Section, Xuanwu Hospital of Capital Medical University, Beijing, China
| | - Jingrong Cao
- Department of Clinical Laboratory, Xuanwu Hospital of Capital Medical University, Beijing, China
| |
Collapse
|
|
9
|
Bongiovanni M, Cavallo C, Barda B, Strulak L, Bernasconi E, Cardia A. Clinical Findings of Listeria monocytogenes Infections with a Special Focus on Bone Localizations. Microorganisms 2024; 12:178. [PMID: 38258004 PMCID: PMC10821090 DOI: 10.3390/microorganisms12010178] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/12/2023] [Revised: 01/05/2024] [Accepted: 01/15/2024] [Indexed: 01/24/2024] Open
Abstract
Listeria monocytogenes is a Gram-positive pathogenic bacterium which can be found in soil or water. Infection with the microorganism can occur after ingestion of contaminated food products. Small and large outbreaks of listeriosis have been described in the past. L. monocytogenes can cause a number of different clinical syndromes, most frequently sepsis, meningitis, and rhombencephalitis, particularly in immunocompromised hosts. L. monocytogenes systemic infections can develop following tissue penetration across the gastrointestinal tract or to hematogenous spread to sterile sites, possibly evolving towards bacteremia. L. monocytogenes only rarely causes bone or joint infections, usually in the context of prosthetic material that can provide a site for bacterial seeding. We describe here the clinical findings of invasive listeriosis, mainly focusing on the diagnosis, clinical management, and treatment of bone and vertebral infections occurring in the context of invasive listeriosis.
Collapse
Affiliation(s)
- Marco Bongiovanni
- Division of Infectious Diseases, Ente Ospedaliero Cantonale, 6900 Lugano, Switzerland; (B.B.); (E.B.)
| | - Claudio Cavallo
- Division of Neurosurgery, Ente Ospedaliero Cantonale, 6900 Lugano, Switzerland; (C.C.); (L.S.)
| | - Beatrice Barda
- Division of Infectious Diseases, Ente Ospedaliero Cantonale, 6900 Lugano, Switzerland; (B.B.); (E.B.)
| | - Lukasz Strulak
- Division of Neurosurgery, Ente Ospedaliero Cantonale, 6900 Lugano, Switzerland; (C.C.); (L.S.)
| | - Enos Bernasconi
- Division of Infectious Diseases, Ente Ospedaliero Cantonale, 6900 Lugano, Switzerland; (B.B.); (E.B.)
| | - Andrea Cardia
- Division of Neurosurgery, Ente Ospedaliero Cantonale, 6900 Lugano, Switzerland; (C.C.); (L.S.)
| |
Collapse
|
|
10
|
Eallonardo SJ, Freitag NE. Crossing the Barrier: A Comparative Study of Listeria monocytogenes and Treponema pallidum in Placental Invasion. Cells 2023; 13:88. [PMID: 38201292 PMCID: PMC10778170 DOI: 10.3390/cells13010088] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/20/2023] [Revised: 12/03/2023] [Accepted: 12/19/2023] [Indexed: 01/12/2024] Open
Abstract
Vertically transmitted infections are a significant cause of fetal morbidity and mortality during pregnancy and pose substantial risks to fetal development. These infections are primarily transmitted to the fetus through two routes: (1) direct invasion and crossing the placenta which separates maternal and fetal circulation, or (2) ascending the maternal genitourinary tact and entering the uterus. Only two bacterial species are commonly found to cross the placenta and infect the fetus: Listeria monocytogenes and Treponema pallidum subsp. pallidum. L. monocytogenes is a Gram-positive, foodborne pathogen found in soil that acutely infects a wide variety of mammalian species. T. pallidum is a sexually transmitted spirochete that causes a chronic infection exclusively in humans. We briefly review the pathogenesis of these two very distinct bacteria that have managed to overcome the placental barrier and the role placental immunity plays in resisting infection. Both organisms share characteristics which contribute to their transplacental transmission. These include the ability to disseminate broadly within the host, evade immune phagocytosis, and the need for a strong T cell response for their elimination.
Collapse
Affiliation(s)
- Samuel J. Eallonardo
- Department of Microbiology and Immunology, University of Illinois Chicago, Chicago, IL 60612, USA;
| | - Nancy E. Freitag
- Department of Microbiology and Immunology, University of Illinois Chicago, Chicago, IL 60612, USA;
- Department of Pharmaceutical Sciences, University of Illinois Chicago, Chicago, IL 60612, USA
| |
Collapse
|
|
11
|
Charlier C, Barrault Z, Rousseau J, Kermorvant-Duchemin E, Meyzer C, Semeraro M, Fall M, Coulpier G, Leclercq A, Charles MA, Ancel PY, Lecuit M. Long-term neurological and neurodevelopmental outcome of neonatal listeriosis in France: a prospective, matched, observational cohort study. THE LANCET. CHILD & ADOLESCENT HEALTH 2023; 7:875-885. [PMID: 37871603 DOI: 10.1016/s2352-4642(23)00195-5] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/11/2023] [Revised: 06/20/2023] [Accepted: 07/24/2023] [Indexed: 10/25/2023]
Abstract
BACKGROUND Maternal-neonatal listeriosis is a rare and serious infection. The long-term outcome of surviving infants with early-onset or late-onset listeriosis remains unknown. We aimed to determine the long-term neurological and neurodevelopmental outcome of neonatal listeriosis. METHODS In this prospective, matched, observational cohort study, we evaluated children born with microbiologically confirmed maternal-neonatal listeriosis in the French MONALISA cohort. At age 5 years, children underwent neurological and neurodevelopmental assessments of sensory deficits, executive function, adaptive behaviour, and cognitive and motor coordination function. The cognitive domain was assessed using the French version of the Wechsler Preschool and Primary Scale of Intelligence, fourth edition, and scored by Full Scale Intelligence Quotient (FSIQ). The motor domain was assessed by physical examination designed to screen for cerebral palsy and developmental coordination disorder. Executive functioning was assessed using the statue and inhibition subtests of Neuropsychological Assessment, second version. The sensory domain was assessed by parental interview, medical report, and clinical assessment. Adaptive behaviour was measured using the Vineland-II behaviour scale from parent-reported assessments of functional communication, socialisation, daily living, and motor skills. Results were compared with gestational age-matched children from two national prospective cohorts: EPIPAGE-2 (preterm infants) and ELFE (term infants from a general population of infants >32 weeks gestation). This study is registered with ClinicalTrials.gov (NCT02580812). FINDINGS Of 59 children who were alive and eligible to participate in the study, 53 (median age 5 years, IQR 5-6) were enrolled for neurodevelopmental assessments between Oct 26, 2016, and Oct 29, 2019. Of 53 children, 31 (58%) had been born preterm, 22 (42%) had early-onset systemic infection, 18 (34%) had early-onset non-systemic infection, and six (11%) had late-onset systemic infection, all with meningitis. 29 (66%) of 44 children, in whom neurodevelopmental disabilities scores were available, developed at least one disability; eight (18%) children had severe neurodevelopmental disabilities. Of four children with late-onset infection and in whom neurodevelopmental disabilities scores were available, three developed at least one neurodevelopmental disability. Neurological and neurodevelopmental outcomes of children with neonatal listeriosis did not differ from those of gestational age-matched control children without infection (relative risk [RR] of at least one disability 0·99 [95% CI 0·65-1·51; p=0·97]; RR of FSIQ less than -1 SD 0·92 [0·54-1·54; p=0·74]). INTERPRETATION These results highlight the burden of persistent disability and dominant contribution of prematurity to long-term outcomes in children born with neonatal listeriosis. The findings support the implementation of systematic long-term screening and provision of tailored education and special needs support. FUNDING Institut Pasteur, Inserm, French Public Health Agency, Contrat de Recherche Clinique, and Assistance Publique-Hôpitaux de Paris.
Collapse
Affiliation(s)
- Caroline Charlier
- Biology of Infection Unit, Inserm U1117, Institut Pasteur-Université Paris Cité, Paris, France; Université Paris Cité, Paris, France; French National Reference Centre and WHO Collaborating Centre Listeria, Institut Pasteur, Paris, France; Department of Infectious Diseases and Tropical Medicine, Cochin Port Royal University Hospital, AP-HP, Paris, France.
| | - Zoé Barrault
- French National Reference Centre and WHO Collaborating Centre Listeria, Institut Pasteur, Paris, France
| | - Jessica Rousseau
- CRESS, Obstetrical Perinatal and Paediatric Epidemiology Research Team, EPOPé, INSERM, INRAE, Paris, France; Clinical Research Unit, Centre for Clinical Investigation P1419, Assistance Publique-Hôpitaux de Paris, Paris, France
| | - Elsa Kermorvant-Duchemin
- Université Paris Cité, Paris, France; Department of Neonatology, Necker-Enfants Malades University Hospital, AP-HP, Paris, France
| | - Candice Meyzer
- Clinical Research Unit, Centre for Clinical Investigation P1419, Assistance Publique-Hôpitaux de Paris, Paris, France
| | - Michaela Semeraro
- Université Paris Cité, Paris, France; Clinical Research Unit, Centre for Clinical Investigation P1419, Assistance Publique-Hôpitaux de Paris, Paris, France
| | - Magatte Fall
- French National Reference Centre and WHO Collaborating Centre Listeria, Institut Pasteur, Paris, France
| | - Gabrielle Coulpier
- French National Reference Centre and WHO Collaborating Centre Listeria, Institut Pasteur, Paris, France
| | - Alexandre Leclercq
- French National Reference Centre and WHO Collaborating Centre Listeria, Institut Pasteur, Paris, France
| | - Marie-Aline Charles
- CRESS, Obstetrical Perinatal and Paediatric Epidemiology Research Team, EPOPé, INSERM, INRAE, Paris, France; Unité Mixte Inserm-Ined-EFS ELFE, Ined, 93300 Aubervilliers, France
| | - Pierre-Yves Ancel
- CRESS, Obstetrical Perinatal and Paediatric Epidemiology Research Team, EPOPé, INSERM, INRAE, Paris, France; Université Paris Cité, Paris, France
| | - Marc Lecuit
- Biology of Infection Unit, Inserm U1117, Institut Pasteur-Université Paris Cité, Paris, France; Université Paris Cité, Paris, France; French National Reference Centre and WHO Collaborating Centre Listeria, Institut Pasteur, Paris, France; Department of Infectious Diseases and Tropical Medicine, Necker-Enfants Malades University Hospital, AP-HP, Paris, France.
| |
Collapse
|
|
12
|
Zhang S, Li J, Wan L, Yu J, Chen S, Jin Z. Clinical Features and Antibiotic Treatment of Neonatal Listeriosis: A Hospital-Based Study. Infect Drug Resist 2023; 16:6647-6659. [PMID: 37849792 PMCID: PMC10577243 DOI: 10.2147/idr.s431402] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/15/2023] [Accepted: 10/04/2023] [Indexed: 10/19/2023] Open
Abstract
Purpose To determine the clinical features, laboratory findings, antibiotic treatment, and outcomes of neonatal listeriosis in a specialized tertiary hospital in Wuhan, China. Patients and Methods We retrospectively analyzed the medical records of patients diagnosed with neonatal listeriosis at Maternal and Child Health Hospital of Hubei Province from January 2015 to December 2022. Listeriosis was indicated by positive culture for Listeria monocytogenes (LM). Results A total of 11 cases were included in our study. The incidence rate of neonatal listeriosis was 2.06 per 100,000 live births. Seventy-three percent of the cases were born prematurely, caused early onset sepsis. Respiratory distress (100%) was the most common and earliest symptom, followed by fever (64%) and rashes (27%). The levels of C-reactive protein (CRP) and procalcitonin (PCT) were elevated in 100% of the cases. The median time-to-positivity (TTP) of the culture was 15 hours (range 9-28hours). Of the 11 neonates, 6 were cured, 2 showed improvement, and 3 died, with a mortality rate of 27%. There were statistically significant differences in Apgar score at 5 minutes (p=0.037) and CRP (p=0.025) between the survival group and fatality group. Ampicillin was sensitive to LM isolates and effective for therapy if initiated early. Conclusion Neonatal listeriosis is a rare but severe infection with a high mortality rate. Early identification and appropriate use of effective antibiotics are particularly critical for achieving positive outcomes. Apgar score and CRP are relevant indices for prognosis. Ampicillin is the first-line therapy and can be empirically administered to neonates suspected of having listeriosis.
Collapse
Affiliation(s)
- Shasha Zhang
- Department of Pharmacy, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430070, People’s Republic of China
| | - Jinchun Li
- Department of Neonatology, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430070, People’s Republic of China
| | - Li Wan
- Department of Pharmacy, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430070, People’s Republic of China
| | - Jing Yu
- Department of Neonatology, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430070, People’s Republic of China
| | - Sumei Chen
- Department of Neonatology, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430070, People’s Republic of China
| | - Zhengjiang Jin
- Department of Clinical Laboratory, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430070, People’s Republic of China
| |
Collapse
|
|
13
|
Correia de Sá A, Casanova D, Ferreira AL, Fernandes C, Cotter J. Listeriosis in Pregnancy: A Rare but High-Risk Infection. Cureus 2023; 15:e47748. [PMID: 38021504 PMCID: PMC10676268 DOI: 10.7759/cureus.47748] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 10/26/2023] [Indexed: 12/01/2023] Open
Abstract
Listeriosis is a rare infection among the general population, with an estimated incidence in Europe of 0.49 cases per 100,000 habitants in 2021. During pregnancy, the incidence rises around ten times, peaking in the third trimester. While maternal consequences are usually mild, the potential for severe fetal and neonatal outcomes exists, leading to fetal loss, prematurity, neonatal sepsis, meningitis, and mortality. In the newborn, the clinical presentation and outcomes are associated with both gestational timing of infection and birth gestational age. We report a case of a pregnant woman with fever and nonspecific symptoms during the second trimester, leading to the diagnosis of Listeria bacteremia. We describe the steps for diagnostics, evolution, and complications and the importance of the differential diagnosis when evaluating pregnant patients.
Collapse
Affiliation(s)
| | - Daniela Casanova
- Internal Medicine, Hospital da Senhora da Oliveira, Guimarães, PRT
| | - Ana L Ferreira
- Internal Medicine, Hospital da Senhora da Oliveira, Guimarães, PRT
| | - Carlos Fernandes
- Internal Medicine, Hospital da Senhora da Oliveira, Guimarães, PRT
| | - Jorge Cotter
- Internal Medicine, Hospital da Senhora da Oliveira, Guimarães, PRT
| |
Collapse
|
|
14
|
Charlier C, Noel C, Hafner L, Moura A, Mathiaud C, Pitsch A, Meziane C, Jolly-Sanchez L, de Pontfarcy A, Diamantis S, Bracq-Dieye H, Disson O, Thouvenot P, Valès G, Tessaud-Rita N, Tourdjman M, Leclercq A, Lecuit M. Fatal neonatal listeriosis following L. monocytogenes horizontal transmission highlights neonatal susceptibility to orally acquired listeriosis. Cell Rep Med 2023; 4:101094. [PMID: 37385252 PMCID: PMC10394164 DOI: 10.1016/j.xcrm.2023.101094] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/29/2022] [Revised: 04/18/2023] [Accepted: 06/02/2023] [Indexed: 07/01/2023]
Abstract
We report a case of fulminant fatal neonatal listeriosis due to horizontal transmission of Listeria monocytogenes (Lm) in a neonatal double room. Genomic analyses reveal a close genetic relationship between clinical isolates, supporting cross-contamination. Oral inoculation experiments in adult and neonatal mice show that neonates are susceptible to a low Lm inoculum and that this susceptibility results from the immaturity of the neonatal gut microbiota. Infected neonates should therefore be isolated for as long as they shed Lm in their feces to avoid horizontal transmission and its dire consequences.
Collapse
Affiliation(s)
- Caroline Charlier
- Institut Pasteur, Université Paris Cité, INSERM U1117, Biology of Infection Unit, 75015 Paris, France; Institut Pasteur, National Reference Center and WHO Collaborating Center Listeria, 75015 Paris, France; Cochin University Hospital, Division of Infectious Diseases and Tropical Medicine, APHP, Institut Imagine, 75015 Paris, France.
| | - Coralie Noel
- Groupe Hospitalier Sud Ile-de-France, 77000 Melun, France
| | - Lukas Hafner
- Institut Pasteur, Université Paris Cité, INSERM U1117, Biology of Infection Unit, 75015 Paris, France
| | - Alexandra Moura
- Institut Pasteur, Université Paris Cité, INSERM U1117, Biology of Infection Unit, 75015 Paris, France; Institut Pasteur, National Reference Center and WHO Collaborating Center Listeria, 75015 Paris, France
| | | | - Aurélia Pitsch
- Groupe Hospitalier Sud Ile-de-France, 77000 Melun, France
| | - Chakib Meziane
- Groupe Hospitalier Sud Ile-de-France, 77000 Melun, France
| | | | | | | | - Hélène Bracq-Dieye
- Institut Pasteur, National Reference Center and WHO Collaborating Center Listeria, 75015 Paris, France
| | - Olivier Disson
- Institut Pasteur, Université Paris Cité, INSERM U1117, Biology of Infection Unit, 75015 Paris, France
| | - Pierre Thouvenot
- Institut Pasteur, National Reference Center and WHO Collaborating Center Listeria, 75015 Paris, France
| | - Guillaume Valès
- Institut Pasteur, National Reference Center and WHO Collaborating Center Listeria, 75015 Paris, France
| | - Nathalie Tessaud-Rita
- Institut Pasteur, National Reference Center and WHO Collaborating Center Listeria, 75015 Paris, France
| | | | - Alexandre Leclercq
- Institut Pasteur, National Reference Center and WHO Collaborating Center Listeria, 75015 Paris, France
| | - Marc Lecuit
- Institut Pasteur, Université Paris Cité, INSERM U1117, Biology of Infection Unit, 75015 Paris, France; Institut Pasteur, National Reference Center and WHO Collaborating Center Listeria, 75015 Paris, France; Necker-Enfants Malades University Hospital, Division of Infectious Diseases and Tropical Medicine, APHP, Institut Imagine, 75015 Paris, France.
| |
Collapse
|
|
15
|
Servarayan K, Krishnamoorthy G, Sundaram E, Karuppusamy M, Murugan M, Piraman S, Vasantha VS. Optical Immunosensor for the Detection of Listeria monocytogenes in Food Matrixes. ACS OMEGA 2023; 8:15979-15989. [PMID: 37179640 PMCID: PMC10173425 DOI: 10.1021/acsomega.2c07848] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 12/09/2022] [Accepted: 04/10/2023] [Indexed: 05/15/2023]
Abstract
In this paper, simple imine-based organic fluorophore 4-amino-3-(anthracene-9 yl methyleneamino) phenyl (phenyl) methanone (APM) has been synthesized via a greener approach and the same was used to construct a fluorescent immunoassay for the detection of Listeria monocytogenes (LM). A monoclonal antibody of LM was tagged with APM via the conjugation of the amine group in APM and the acid group of anti-LM through EDC/NHS coupling. The designed immunoassay was optimized for the specific detection of LM in the presence of other interfering pathogens based on the aggregation-induced emission mechanism and the formation of aggregates and their morphology was confirmed with the help of scanning electron microscopy. Density functional theory studies were done to further support the sensing mechanism-based changes in the energy level distribution. All photophysical parameters were measured by using fluorescence spectroscopy techniques. Specific and competitive recognition of LM was done in the presence of other relevant pathogens. The immunoassay shows a linear appreciable range from 1.6 × 106-2.7024 × 108 cfu/mL using the standard plate count method. The LOD has been calculated from the linear equation and the value is found as 3.2 cfu/mL, and this is the lowest LOD value reported for the detection of LM so far. The practical applications of the immunoassay were demonstrated in various food samples, and their accuracy obtained was highly comparable with the standard existing ELISA method.
Collapse
Affiliation(s)
- Karthika
Lakshmi Servarayan
- Department
of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India
| | - Govindan Krishnamoorthy
- Translational
Research Platform for Veterinary Biologicals, Central University Laboratory, TANUVAS, Chennai 600051, Tamil Nadu, India
| | - Ellairaja Sundaram
- Department
of Chemistry, Vivekananda College, Tiruvedakam-West, Madurai 625234, India
| | - Masiyappan Karuppusamy
- Centre
for High Computing, CSIR-Central Leather
Research Institute, Adyar, Chennai 600020, India
- Academy
of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India
| | - Marudhamuthu Murugan
- Department
of Microbial Technology, Madurai Kamaraj
University, Madurai 625021, India
| | - Shakkthivel Piraman
- Department
of Nanoscience and Technology, Alagappa
University, Karaikudi-630003, India
| | - Vairathevar Sivasamy Vasantha
- Department
of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India
| |
Collapse
|
|
16
|
Simão Raimundo D, Viveiros E, Monteiro I, Gomes F. Challenges in Managing a Case of Neonatal Listeriosis in Portugal. Cureus 2023; 15:e38405. [PMID: 37265911 PMCID: PMC10231900 DOI: 10.7759/cureus.38405] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 04/30/2023] [Indexed: 06/03/2023] Open
Abstract
A male neonate was born at 34 weeks due to spontaneous labor with associated fetal distress and meconium-stained amniotic fluid. The neonate presented with septic shock and congenital pneumonia shortly after birth and later neurological symptoms. Listeria monocytogenes was identified in blood samples, but with negative urine and cerebrospinal fluid cultures. The neonate required assisted ventilation for a period of 10 days and received high-dose and long-term antibiotic therapy. Despite the fact that the mother denied an infectious risk for listeriosis infection, she developed mild respiratory symptoms. Her microbiological investigation was negative, although it did not include placental samples. Vertical transmission in this case was presumed but not confirmed. The newborn was discharged asymptomatic at day 26 of life and has presented normal developmental evolution until present, at eight months old. Listeria monocytogenes is a classic but relatively rare cause of neonatal sepsis and meningitis. This case describes a clinically successfully managed case with no possible epidemiological link and illustrates the challenges in managing cases of a public health disease. In neonatal listeriosis, communication between Neonatology and Obstetrics departments, as well as with public health, is vital, and long-term follow-up is crucial to identify possible neurological sequelae.
Collapse
Affiliation(s)
- Diana Simão Raimundo
- Pediatrics Department, Hospital do Divino Espírito Santo de Ponta Delgada, Ponta Delgada, PRT
| | - Eulália Viveiros
- Neonatal Unit, Pediatrics Department, Hospital do Divino Espírito Santo de Ponta Delgada, Ponta Delgada, PRT
| | - Isabel Monteiro
- Neonatal Unit, Pediatrics Department, Hospital do Divino Espírito Santo de Ponta Delgada, Ponta Delgada, PRT
| | - Fernanda Gomes
- Neonatal Unit, Pediatrics Department, Hospital do Divino Espírito Santo de Ponta Delgada, Ponta Delgada, PRT
| |
Collapse
|
|
17
|
Scarponi D, ranalli M, Bedetti L, Miselli F, Rossi K, Iughetti L, Lugli L, Berardi A. La listeriosi neonatale e materno-fetale. MEDICO E BAMBINO 2023; 42:177-182. [DOI: 10.53126/meb42177] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 09/18/2023]
Abstract
Listeriosis is a foodborne condition associated with the ingestion of food contaminated by Listeria monocytogenes that can cause serious health consequences in neonates and pregnant women. In these groups the incidence of the disease is higher due to the relative immunocompromised state and the particular Listeria monocytogenes tropism for the placenta. The paediatrician and the neonatologist must therefore be able to recognize the signs and symptoms of the disease promptly so as to set up an adequate treatment according to the most recent international guidelines. This article provides epidemiological data and describes the pathophysiology and clinical manifestations of maternal-foetal and neonatal listeriosis, as well as the therapeutic indications according to very recent recommendations.
Collapse
Affiliation(s)
- Davide Scarponi
- Scuola di Specializzazione in Pediatria, AOU Policlinico di Modena
| | - Marco ranalli
- Scuola di Specializzazione in Pediatria, AOU Policlinico di Modena
| | - Luca Bedetti
- Terapia Intensiva Neonatale, AOU Policlinico di Modena
| | | | - katia Rossi
- Terapia Intensiva Neonatale, AOU Policlinico di Modena
| | | | - Licia Lugli
- Terapia Intensiva Neonatale, AOU Policlinico di Modena
| | | |
Collapse
|
|
18
|
Abstract
Listeria monocytogenes is a Gram-positive facultative intracellular pathogen that can cause severe invasive infections upon ingestion with contaminated food. Clinically, listerial disease, or listeriosis, most often presents as bacteremia, meningitis or meningoencephalitis, and pregnancy-associated infections manifesting as miscarriage or neonatal sepsis. Invasive listeriosis is life-threatening and a main cause of foodborne illness leading to hospital admissions in Western countries. Sources of contamination can be identified through international surveillance systems for foodborne bacteria and strains' genetic data sharing. Large-scale whole genome studies have increased our knowledge on the diversity and evolution of L. monocytogenes, while recent pathophysiological investigations have improved our mechanistic understanding of listeriosis. In this article, we present an overview of human listeriosis with particular focus on relevant features of the causative bacterium, epidemiology, risk groups, pathogenesis, clinical manifestations, and treatment and prevention.
Collapse
Affiliation(s)
- Merel M Koopmans
- Amsterdam UMC, University of Amsterdam, Department of Neurology, Amsterdam Neuroscience, Amsterdam, the Netherlands
| | - Matthijs C Brouwer
- Amsterdam UMC, University of Amsterdam, Department of Neurology, Amsterdam Neuroscience, Amsterdam, the Netherlands
| | - José A Vázquez-Boland
- Infection Medicine, Edinburgh Medical School (Biomedical Sciences), University of Edinburgh, Edinburgh, United Kingdom
| | - Diederik van de Beek
- Amsterdam UMC, University of Amsterdam, Department of Neurology, Amsterdam Neuroscience, Amsterdam, the Netherlands
| |
Collapse
|
|
19
|
Germanopoulou T, Ishak A, Hardy EJ, Johnson JE, Mylonakis E. Listeriosis in Pregnancy. CURRENT TREATMENT OPTIONS IN INFECTIOUS DISEASES 2023. [DOI: 10.1007/s40506-023-00262-x] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 03/03/2023]
|
|
20
|
Yu L, Zhang Y, Qi X, Bai K, Zhang Z, Bu H. Next-generation sequencing for the diagnosis of Listeria monocytogenes meningoencephalitis: a case series of five consecutive patients. J Med Microbiol 2023; 72. [PMID: 36748504 DOI: 10.1099/jmm.0.001641] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/11/2023] Open
Abstract
Introduction. The prompt and specific diagnosis of Listeria monocytogenes meningoencephalitis (LMM) is challenging. Next-generation sequencing (NGS) of cerebrospinal fluid (CSF) is an emerging technique for diagnosing infrequent causative pathogens.Hypothesis/Gap statement. We hypothesized that NGS of CSF is an effective approach for diagnosing LMM.Aim. To evaluate the effectiveness of NGS, we present five cases of LMM diagnosed using NGS of the CSF.Methodology. Between August 2017 and 30 September 2020, we used NGS of the CSF to detect pathogens in patients with clinically suspected central nervous system infections. The clinical characteristics, laboratory tests, imaging findings and NGS results are reviewed.Results. Five patients were diagnosed with LMM using NGS of the CSF within 2 to 4 days, although the clinical manifestations, medical history and imaging findings varied strikingly. NGS of CSF showed sequence reads corresponding to L. monocytogenes species ranging from 118 to 1997 bp, genomic coverage of 0.29-5.96 %, relative abundance of 14.83-32.16 % and sequencing depth of 1.12 to 1.35. The prompt diagnosis resulted in targeted and effective treatment with the appropriate antibiotics, although two patients with the most severe cerebral parenchymal lesions showed little improvement.Conclusion. Our results demonstrate the power of NGS of CSF for the prompt diagnosis of LMM. NGS of CSF is an important complementary tool for identifying L. monocytogenes.
Collapse
Affiliation(s)
- Lili Yu
- Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, PR China.,Key Laboratory of Neurology of Hebei Province, Shijiazhuang 050000, Hebei, PR China
| | - Yu Zhang
- Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, PR China.,Key Laboratory of Neurology of Hebei Province, Shijiazhuang 050000, Hebei, PR China
| | - Xuejiao Qi
- Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, PR China.,Key Laboratory of Neurology of Hebei Province, Shijiazhuang 050000, Hebei, PR China
| | - Kaixuan Bai
- Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, PR China.,Key Laboratory of Neurology of Hebei Province, Shijiazhuang 050000, Hebei, PR China
| | - Zhenyuan Zhang
- Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, PR China.,Key Laboratory of Neurology of Hebei Province, Shijiazhuang 050000, Hebei, PR China
| | - Hui Bu
- Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, PR China.,Key Laboratory of Neurology of Hebei Province, Shijiazhuang 050000, Hebei, PR China
| |
Collapse
|
|
21
|
Zhan Y, Xu T, Liu H, Wang X, Yu H. Perinatal Infection with Listeria monocytogenes: A 10-Year Hospital-Based Study in Western China. J Inflamm Res 2023; 16:1243-1254. [PMID: 36987517 PMCID: PMC10040159 DOI: 10.2147/jir.s401171] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/12/2022] [Accepted: 03/16/2023] [Indexed: 03/30/2023] Open
Abstract
Objective Listeriosis is a rare but severe foodborne disease that is contracted by the consumption of food contaminated with Listeria, and perinatal listeriosis can cause major adverse pregnancy outcomes. In the current study, we sought to retrospectively analyze 10 years of perinatal outcomes due to listeriosis in western China. Methods Based on the isolation of Listeria monocytogenes (L. monocytogenes), we retrospectively reviewed the pregnancy-associated cases of listeriosis from June 2010 to February 2022 at our hospital. We investigated the crude incidence, clinical manifestations, perinatal management, and outcomes of perinatal listeriosis cases. Results We diagnosed a total of 28 cases of perinatal listeriosis during the study period at our hospital, including four cases of twin pregnancies. Of the 28 cases, 32.14% (9/28) were infected in the second trimester and 67.86% (19/28) in the third trimester. The fetal or neonatal mortality rate for perinatal listeriosis was high, with a crude fetal mortality rate of 46.88% (15/32) in the 32 offspring and a neonatal mortality rate of 47.06% (8/17) in the 17 live births. Conclusion Perinatal listeriosis exhibits high fetal or neonatal mortality. We recommend that appropriate antibiotics be administered in cases of perinatal listeriosis.
Collapse
Affiliation(s)
- Yongchi Zhan
- Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, People’s Republic of China
- Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, People’s Republic of China
| | - Tingting Xu
- Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, People’s Republic of China
- Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, People’s Republic of China
| | - Hongyan Liu
- Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, People’s Republic of China
- Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, People’s Republic of China
| | - Xiaodong Wang
- Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, People’s Republic of China
- Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, People’s Republic of China
| | - Haiyan Yu
- Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, People’s Republic of China
- Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, People’s Republic of China
- Correspondence: Haiyan Yu; Xiaodong Wang, Department of Obstetrics and Gynecology, West China Second University Hospital; Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, No. 20, 3rd Section, South Renmin Road, Chengdu, Sichuan, 610041, People’s Republic of China, Email ;
| |
Collapse
|
|
22
|
Iiyambo OJ, Baba V, Thomas J, Sekwadi P, Naidoo P. Presentation and outcomes of Listeria-affected pregnancies in Johannesburg tertiary hospitals: A 2-year review. Int J Gynaecol Obstet 2022; 161:989-996. [PMID: 36495249 DOI: 10.1002/ijgo.14617] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/08/2022] [Revised: 06/08/2022] [Accepted: 12/07/2022] [Indexed: 12/14/2022]
Abstract
OBJECTIVE To describe maternal risk factors, presentations, peripartum findings, and pregnancy outcomes in Listeria monocytogenes-infected women. METHODS A retrospective descriptive case review. The records of 51 pregnant women infected with listeriosis who delivered infants between February 1, 2016 and February 28, 2018 at three academic hospitals in Johannesburg, South Africa, were included. The diagnosis of listeriosis was made on maternal/neonatal-sampled blood or tissue cultures. RESULTS Forty-eight (82.3%) Listeria infections of maternal and neonatal listeriosis were diagnosed on blood culture. The median gestational age at diagnosis was at a preterm gestation of 33 (20-43) weeks. Twenty-eight women (54.9%) had normal vaginal deliveries. Precipitous labor was described in 18 (39%) of these women. Fetal distress was the indication for cesarean section in 22 (41.2%) women. Meconium-stained amniotic fluid was found in 21 (61.7%) women at the time of delivery. The category of very low birth weight had 14 (27.4%) neonates with an Apgar score of less than 7 at 5 min. Maternal morbidities included chorioamnionitis (3 [5.8%]) and puerperal infections (7 [13.7%]). The HIV-positive anemic women had a tendency towards listerial infections. CONCLUSIONS Symptoms of listeriosis were non-specific and diagnosis was detected on blood culture sampling. Risk factors included HIV seropositivity and were associated with puerperal infections and anemia.
Collapse
Affiliation(s)
- Olivia-Joan Iiyambo
- Department of Obstetrics and Gynaecology, Chris Hani Baragwanath Academic Hospital, University of the Witwatersrand, Johannesburg, South Africa
| | - Vuyelwa Baba
- Department of Obstetrics and Gynaecology, Chris Hani Baragwanath Academic Hospital, University of the Witwatersrand, Johannesburg, South Africa
| | - Juno Thomas
- Centre for Enteric Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa
| | - Phuti Sekwadi
- Centre for Enteric Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa
| | - Poovangela Naidoo
- Department of Obstetrics and Gynaecology, Maternal Fetal Medicine Unit, Chris Hani Baragwanath Academic Hospital, University of the Witwatersrand, Johannesburg, South Africa
| |
Collapse
|
|
23
|
AGBOOLA TD, BISI-JOHNSON MA. OCCURRENCE OF Listeria monocytogenes IN IRRIGATION WATER AND IRRIGATED VEGETABLES IN SELECTED AREAS OF OSUN STATE, NIGERIA. SCIENTIFIC AFRICAN 2022. [DOI: 10.1016/j.sciaf.2022.e01503] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/14/2022] Open
|
|
24
|
Craig A, Federspiel J, Wein L, Thompson J, Dotters-Katz S. Maternal and obstetric outcomes of listeria pregnancy: insights from a national cohort. J Matern Fetal Neonatal Med 2022; 35:10010-10016. [PMID: 35686719 PMCID: PMC9846892 DOI: 10.1080/14767058.2022.2083494] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/24/2021] [Revised: 05/09/2022] [Accepted: 05/20/2022] [Indexed: 01/21/2023]
Abstract
OBJECTIVE We sought to evaluate and describe the maternal and obstetric morbidity associated with Listeria infection in pregnancy. METHODS Retrospective cohort of pregnant women using the 2007-2018 National Inpatient Sample. Pregnant women with discharge diagnosis codes consistent with Listeria infection were identified. Outcomes of deliveries complicated by Listeria infection were compared to those of delivery without this infection. The primary outcome was a composite of severe maternal morbidity. Secondary outcomes included components of the composite, maternal length of stay, mode of delivery, stillbirth, and preterm delivery. RESULTS We identified 134 maternity associated hospitalizations for Listeria (weighted national estimate 666), of which 72 (weighted national estimate of 358) were delivery admissions. Delivery admissions complicated by Listeria resulted in higher rates of severe maternal morbidity than those without, (30.9% vs. 1.6%, p<.001). In adjusted analyses, women with Listeria had 21.2-fold higher risk of severe maternal morbidity (95% CI: 14.0, 31.9) when compared to those without Listeria. Specifically, Listeria delivery admissions had higher rates of acute respiratory distress syndrome (2.8% vs. 0.1%, p<.001), mechanical ventilation (1.4% vs. 0.0%, p<.001), sepsis (28.1% vs. 0.1%, p<.001), and shock (1.4% vs. 0.0%, p<.001). Listeria delivery admissions also had higher rates of preterm birth (61.3% vs. 7.7%, p < 0.001) and stillbirth (13.5% vs. 0.7%, p<.001). Women hospitalized or delivered with Listeria infection were also more likely to have a cesarean delivery (57.9% vs. 32.9, p<.001) and the average length of stay for women with Listeria was also longer (4.0 days vs. 2.3 days, p<.001). CONCLUSIONS Women with Listeria infection in pregnancy have higher rates of severe maternal morbidity, specifically increased risk of sepsis, septic shock, and acute respiratory distress syndrome. Among delivery hospitalizations, these women also have higher rates of preterm birth and stillbirth.
Collapse
Affiliation(s)
- Amanda Craig
- Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC, USA
| | - Jerome Federspiel
- Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC, USA
- Department of Gynecology and Obstetrics, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
| | - Lauren Wein
- Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC, USA
| | - Jennifer Thompson
- Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Vanderbilt University Medical Center, Nashville, TN, USA
| | - Sarah Dotters-Katz
- Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC, USA
| |
Collapse
|
|
25
|
Pogreba-Brown K, Boyd K, Schaefer K, Austhof E, Armstrong A, Owusu-Dommey A, Villa-Zapata L, Arora M, McClelland JD, Hoffman S. Complications Associated with Foodborne Listeriosis: A Scoping Review. Foodborne Pathog Dis 2022; 19:725-743. [PMID: 36367547 DOI: 10.1089/fpd.2022.0012] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/13/2022] Open
Abstract
Listeria monocytogenes is a relatively rare but highly pathogenic bacterium that can cause foodborne infections. In the United States there are ∼1600 cases per year, 94% of which result in hospitalizations and 20% in deaths. Per-case burden is high because the disease also causes serious complications, including sepsis, encephalitis, meningitis, miscarriage, and stillbirth. The disease burden of L. monocytogenes is underestimated because some of these acute complications can also result in long-term outcomes. In this article, we conducted a scoping review of L. monocytogenes complications and longer term outcomes from articles published between 2000 and 2018. Search terms were developed for four major databases (PubMed, Scopus, Web of Science, and Embase) as well as gray literature and hand searches of review articles. We follow standard scoping review methodology and assessment. Out of 10,618 unique articles originally identified, 115 articles were included, representing 49 unique outcomes. The majority of studies were cohort designs (n = 67) and conducted in the United States or Europe (n = 98). Four major outcome groupings were death, neurological disorders, sepsis, and congenital infection. This study identifies substantial research on the common acute complications of L. monocytogenes and few long-term consequences of L. monocytogenes. We identify the need for additional studies to determine the longer term impacts of these acute complications.
Collapse
Affiliation(s)
- Kristen Pogreba-Brown
- Department of Epidemiology and Biostatistics, The University of Arizona, Tucson, Arizona, USA
| | - Kylie Boyd
- Department of Epidemiology and Biostatistics, The University of Arizona, Tucson, Arizona, USA
| | - Kenzie Schaefer
- Department of Epidemiology and Biostatistics, The University of Arizona, Tucson, Arizona, USA
| | - Erika Austhof
- Department of Epidemiology and Biostatistics, The University of Arizona, Tucson, Arizona, USA
| | - Alexandra Armstrong
- Department of Epidemiology and Biostatistics, The University of Arizona, Tucson, Arizona, USA
| | - Ama Owusu-Dommey
- Department of Epidemiology and Biostatistics, The University of Arizona, Tucson, Arizona, USA
| | | | - Mona Arora
- Department of Community, Environment and Policy, The University of Arizona, Tucson, Arizona, USA
| | - Jean D McClelland
- Arizona Health Sciences Library, The University of Arizona, Tucson, Arizona, USA
| | - Sandra Hoffman
- U.S. Department of Agriculture, Washington, District of Columbia, USA
| |
Collapse
|
|
26
|
Rhombencephalitis in Pregnancy-A Challenging Case of Probable Listeria Infection. Life (Basel) 2022; 12:life12101600. [PMID: 36295036 PMCID: PMC9604870 DOI: 10.3390/life12101600] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/15/2022] [Revised: 09/29/2022] [Accepted: 10/11/2022] [Indexed: 11/05/2022] Open
Abstract
Rhombencephalitis refers to inflammation of the brainstem and cerebellum, and can be caused by infections, autoimmune disorders or paraneoplastic syndromes. The most common infective cause is the bacterium Listeria monocytogenes. Listeria monocytogenes is the predominant species to cause human listeriosis, and is commonly due to the ingestion of contaminated foods. Symptoms include a mild gastroenteritis, fever (often with extreme temperature variations), headache, and myalgia. In more severe cases, invasive disease may lead to bacteraemia and neurolisteriosis. Pregnant women are more susceptible to listeriosis, which is believed to be due to pregnancy-related immune modulation. Maternal-neonatal infection with adverse pregnancy outcomes include neonatal listeriosis, spontaneous miscarriage and intrauterine fetal demise. Diagnosis may be challenging due to initial nonspecific symptoms and low sensitivity and specificity of confirmatory diagnostic laboratory tests. Here, we describe a case of rhombencephalitis in pregnancy, attributed to Listeria, and review the clinical features, diagnosis and multidisciplinary management. Lastly, we describe the immunological response to Listeria monocytogenes and show in vitro pro-inflammatory effects of Listeria monocytogenes on peripheral blood mononuclear cells and placental explants.
Collapse
|
|
27
|
Infection of the murine placenta by Listeria monocytogenes induces sex-specific responses in the fetal brain. Pediatr Res 2022; 93:1566-1573. [PMID: 36127406 DOI: 10.1038/s41390-022-02307-1] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 04/29/2022] [Revised: 08/06/2022] [Accepted: 08/30/2022] [Indexed: 11/09/2022]
Abstract
BACKGROUND Epidemiological data indicate that prenatal infection is associated with an increased risk of several neurodevelopmental disorders in the progeny. These disorders display sex differences in presentation. The role of the placenta in the sex-specificity of infection-induced neurodevelopmental abnormalities is not well-defined. We used an imaging-based animal model of the bacterial pathogen Listeria monocytogenes to identify sex-specific effects of placental infection on neurodevelopment of the fetus. METHODS Pregnant CD1 mice were infected with a bioluminescent strain of Listeria on embryonic day 14.5 (E14.5). Excised fetuses were imaged on E18.5 to identify the infected placentas. The associated fetal brains were analyzed for gene expression and altered brain structure due to infection. The behavior of adult offspring affected by prenatal Listeria infection was analyzed. RESULTS Placental infection induced sex-specific alteration of gene expression patterns in the fetal brain and resulted in abnormal cortical development correlated with placental infection levels. Furthermore, male offspring exhibited abnormal social interaction, whereas females exhibited elevated anxiety. CONCLUSION Placental infection by Listeria induced sex-specific abnormalities in neurodevelopment of the fetus. Prenatal infection also affected the behavior of the offspring in a sex-specific manner. IMPACT Placental infection with Listeria monocytogenes induces sexually dichotomous gene expression patterns in the fetal brains of mice. Abnormal cortical lamination is correlated with placental infection levels. Placental infection results in autism-related behavior in male offspring and heightened anxiety levels in female offspring.
Collapse
|
|
28
|
Vidovic S, Paturi G, Gupta S, Fletcher GC. Lifestyle of Listeria monocytogenes and food safety: Emerging listericidal technologies in the food industry. Crit Rev Food Sci Nutr 2022; 64:1817-1835. [PMID: 36062812 DOI: 10.1080/10408398.2022.2119205] [Citation(s) in RCA: 15] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/03/2022]
Abstract
Listeria monocytogenes, a causative agent of listeriosis, is a major foodborne pathogen. Among pathogens, L. monocytogenes stands out for its unique ecological and physiological characteristics. This distinct lifestyle of L. monocytogenes has a significant impact on food safety and public health, mainly through the ability of this pathogen to multiply at refrigeration temperature and to persist in the food processing environment. Due to a combination of these characteristics and emerging trends in consumer preference for ready-to-eat and minimally processed food, there is a need to develop effective and sustainable approaches to control contamination of food products with L. monocytogenes. Implementation of an efficient and reliable control strategy for L. monocytogenes must first address the problem of cross-contamination. Besides the preventive control strategies, cross-contamination may be addressed with the introduction of emerging post packaging non-thermal or thermal hurdles that can ensure delivery of a listericidal step in a packed product without interfering with the organoleptic characteristics of a food product. This review aims to present the most relevant findings underlying the distinct lifestyle of L. monocytogenes and its impact on food safety. We also discuss emerging food decontamination technologies that can be used to better control L. monocytogenes.
Collapse
Affiliation(s)
- Sinisa Vidovic
- Food Safety Preservation Team, The New Zealand Institute for Plant and Food Research Limited, Auckland, New Zealand
| | - Gunaranjan Paturi
- Food Safety Preservation Team, The New Zealand Institute for Plant and Food Research Limited, Auckland, New Zealand
| | - Sravani Gupta
- Food Safety Preservation Team, The New Zealand Institute for Plant and Food Research Limited, Auckland, New Zealand
| | - Graham C Fletcher
- Food Safety Preservation Team, The New Zealand Institute for Plant and Food Research Limited, Auckland, New Zealand
| |
Collapse
|
|
29
|
Khsim IEF, Mohanaraj-Anton A, Horte IB, Lamont RF, Khan KS, Jørgensen JS, Amezcua-Prieto C. Listeriosis in pregnancy: An umbrella review of maternal exposure, treatment and neonatal complications. BJOG 2022; 129:1427-1433. [PMID: 34954888 DOI: 10.1111/1471-0528.17073] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/05/2021] [Revised: 11/15/2021] [Accepted: 11/22/2021] [Indexed: 11/26/2022]
Abstract
BACKGROUND Listeria monocytogenes is a commonly found organism in processed and prepared food and the disease of listeriosis is associated with a high morbidity and mortality. Compared with the general population, the risk of being diagnosed with listeriosis increases during pregnancy. Listeriosis can lead to miscarriage, spontaneous preterm labour and preterm birth, stillbirth and congenital neonatal infections. OBJECTIVES We conducted a universal review of listeriosis in pregnancy and in the newborn. SEARCH STRATEGY The EMBASE, PubMed, Cinahl and Web of Science databases were searched for systematic reviews indexed before 1 December 2020. SELECTION CRITERIA Any systematic reviews evaluating the prevalence, treatment, diagnosis and effects of listeriosis during pregnancy and up to 4 weeks postnatally were included. DATA COLLECTION AND ANALYSIS Eligibility assessment, data extraction and quality assessment by the Methodological Quality Assessment of Systematic Reviews (AMSTAR-2) were performed in duplicate. MAIN RESULTS We identified 397 citations of which nine systematic reviews comprising 330 studies and 487 patients' reviews were included. Most systematic reviews (seven of nine) were of moderate to high quality. Prevention in pregnant women was based on adherence to strict dietary recommendations, such as reheating leftovers until steamed and avoiding unpasteurised dairy products. Listeriosis infections were likely to occur in the third trimester (66%) rather than in the first trimester (3%) of pregnancy. Symptoms are mostly fever and other flu-like symptoms, such as fatigue. Diagnosis was primarily made by culture of the pathogen. Intravenous amoxicillin or ampicillin were first-line treatment. CONCLUSIONS Listeriosis, a rare but serious infectious disease in pregnancy, can cause devastating consequences for the fetus and newborn. Appropriate preventative treatment should be initiated during early pregnancy to avoid complications. TWEETABLE ABSTRACT Listeria is commonly found in processed and prepared food. Prevention is the best way to avoid listeriosis during pregnancy.
Collapse
Affiliation(s)
- Ijlas El Founti Khsim
- Department of Preventive Medicine and Public Health, Faculty of Medicine, University of Granada, Granada, Spain
| | - Ahalini Mohanaraj-Anton
- Department of Gynaecology and Obstetrics, Research Unit of Gynaecology and Obstetrics, Institute of Clinical Research, University of Southern Denmark, Odense, Denmark
| | - Ivar Benjamin Horte
- Department of Gynaecology and Obstetrics, Research Unit of Gynaecology and Obstetrics, Institute of Clinical Research, University of Southern Denmark, Odense, Denmark
| | - Ronald Francis Lamont
- Department of Gynaecology and Obstetrics, Research Unit of Gynaecology and Obstetrics, Institute of Clinical Research, University of Southern Denmark, Odense, Denmark
- Division of Surgery, University College London, London, UK
| | - Khalid Saeed Khan
- Department of Preventive Medicine and Public Health, Faculty of Medicine, University of Granada, Granada, Spain
- Consortium for Biomedical Research in Epidemiology and Public Health (CIBERESP), Madrid, Spain
| | - Jan Stener Jørgensen
- Department of Gynaecology and Obstetrics, Research Unit of Gynaecology and Obstetrics, Institute of Clinical Research, University of Southern Denmark, Odense, Denmark
| | - Carmen Amezcua-Prieto
- Department of Preventive Medicine and Public Health, Faculty of Medicine, University of Granada, Granada, Spain
- Consortium for Biomedical Research in Epidemiology and Public Health (CIBERESP), Madrid, Spain
- Instituto de Investigación Biosanitaria (ibs.Granada), Granada, Spain
| |
Collapse
|
|
30
|
Vallejo P, Cilla G, López-Olaizola M, Vicente D, Marimón JM. Epidemiology and Clinical Features of Listeriosis in Gipuzkoa, Spain, 2010-2020. Front Microbiol 2022; 13:894334. [PMID: 35755994 PMCID: PMC9218358 DOI: 10.3389/fmicb.2022.894334] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/11/2022] [Accepted: 04/29/2022] [Indexed: 12/28/2022] Open
Abstract
Background Listeriosis continues to be one of the most important notifiable foodborne diseases. Nonetheless, in Spain, there are few data on the molecular epidemiology of Listeria monocytogenes infections in recent years. Aim To describe clinical features and the molecular epidemiology of human listeriosis over an 11-year period (2010–2020) in Gipuzkoa, Northern Spain. Methods A total of 111 isolates, all but one from invasive disease, were studied. Serotyping (agglutination and multiplex polymerase chain reaction [PCR]) and multilocus sequence typing were performed for all isolates. Antibiotic susceptibility was assessed by the broth microdilution method. Results The average annual incidence of listeriosis in non-pregnancy-associated cases was 1.55 per 100,000 population, with a 1-month mortality rate of 22.2%. In pregnant women, the average incidence was 0.45 cases per 1,000 pregnancies. Twenty-four sequence types were identified, serotype 4b ST1 (24.3%) being the most frequent followed by 1/2b ST87 (18.9%), which caused two long outbreaks in 2013–2014. A significant association was observed between ST219 and meningitis (p < 0.001). All isolates were susceptible to ampicillin as well as other antibiotics used in listeriosis treatment. Conclusion Despite current control measures, listeriosis continues to be an important cause of mortality in the elderly, preterm birth, and miscarriages in pregnant women. Improvements in the control and diagnosis of listeriosis are needed to reduce the impact of this infection on vulnerable populations.
Collapse
Affiliation(s)
- Pedro Vallejo
- Microbiology Department, Osakidetza Basque Health Service, Donostialdea Integrated Health Organization, San Sebastián, Spain.,Department of Preventive Medicine, University of the Basque Country (UPV/EHU), San Sebastián, Spain
| | - Gustavo Cilla
- Microbiology Department, Infectious Diseases Area, Biodonostia Health Research Institute, Vaccine Preventable Diseases Group, Osakidetza Basque Health Service, Donostialdea Integrated Health Organization, San Sebastián, Spain
| | - Maddi López-Olaizola
- Microbiology Department, Osakidetza Basque Health Service, Donostialdea Integrated Health Organization, San Sebastián, Spain
| | - Diego Vicente
- Department of Preventive Medicine, University of the Basque Country (UPV/EHU), San Sebastián, Spain.,Microbiology Department, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group, Biodonostia Health Research Institute, Osakidetza Basque Health Service, Donostialdea Integrated Health Organization, San Sebastián, Spain
| | - José María Marimón
- Department of Preventive Medicine, University of the Basque Country (UPV/EHU), San Sebastián, Spain.,Microbiology Department, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group, Biodonostia Health Research Institute, Osakidetza Basque Health Service, Donostialdea Integrated Health Organization, San Sebastián, Spain
| |
Collapse
|
|
31
|
Russini V, Spaziante M, Varcasia BM, Diaconu EL, Paolillo P, Picone S, Brunetti G, Mattia D, De Carolis A, Vairo F, Bossù T, Bilei S, De Marchis ML. A Whole Genome Sequencing-Based Epidemiological Investigation of a Pregnancy-Related Invasive Listeriosis Case in Central Italy. Pathogens 2022; 11:667. [PMID: 35745521 PMCID: PMC9228178 DOI: 10.3390/pathogens11060667] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/29/2022] [Revised: 06/03/2022] [Accepted: 06/07/2022] [Indexed: 11/17/2022] Open
Abstract
Listeriosis is currently the fifth most common foodborne disease in Europe. Most cases are sporadic; however, outbreaks have also been reported. Compared to other foodborne infections, listeriosis has a modest incidence but can cause life-threatening complications, especially in elderly or immunocompromised people and pregnant women. In the latter case, the pathology can be the cause of premature birth or spontaneous abortion, especially if the fetus is affected during the first months of gestation. The causative agent of listeriosis, Listeria monocytogenes, is characterized by the innate ability to survive in the environment and in food, even in adverse conditions and for long periods. Ready-to-eat food represents the category most at risk for contracting listeriosis. This study presents the result of an investigation carried out on a case of maternal-fetal transmission of listeriosis which occurred in 2020 in central Italy and which was linked, with a retrospective approach, to other cases residing in the same city of the pregnant woman. Thanks to the use of next-generation sequencing methodologies, it was possible to identify an outbreak of infection, linked to the consumption of ready-to-eat sliced products sold in a supermarket in the investigated city.
Collapse
Affiliation(s)
- Valeria Russini
- Food Microbiology Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, 00178 Rome, Italy; (V.R.); (B.M.V.); (T.B.); (S.B.)
| | - Martina Spaziante
- Regional Service Surveillance and Control for Infectious Diseases (SERESMI), National Institute for Infectious Diseases “Lazzaro Spallanzani” IRCCS, 00149 Rome, Italy; (M.S.); (F.V.)
| | - Bianca Maria Varcasia
- Food Microbiology Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, 00178 Rome, Italy; (V.R.); (B.M.V.); (T.B.); (S.B.)
| | - Elena Lavinia Diaconu
- Department of General Diagnostics, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, 00178 Rome, Italy;
| | - Piermichele Paolillo
- UO Neonatologia, Patologia Neonatale e Terapia Intensiva Neonatale (TIN), Policlinico Casilino General Hospital, 00169 Rome, Italy; (P.P.); (S.P.)
| | - Simonetta Picone
- UO Neonatologia, Patologia Neonatale e Terapia Intensiva Neonatale (TIN), Policlinico Casilino General Hospital, 00169 Rome, Italy; (P.P.); (S.P.)
| | - Grazia Brunetti
- Pathology-Microbiology Laboratory, Policlinico Casilino General Hospital, 00169 Rome, Italy;
| | - Daniela Mattia
- Dipartimento di Prevenzione, Servizio Veterinario Area B—Igiene Alimenti di Origine Animale (SIOA), ASL Roma 6, 00072 Rome, Italy;
| | - Angela De Carolis
- Dipartimento di Prevenzione, Servizio di Igiene degli Alimenti e della Nutrizione (SIAN), ASL Roma 6, 00044 Rome, Italy;
| | - Francesco Vairo
- Regional Service Surveillance and Control for Infectious Diseases (SERESMI), National Institute for Infectious Diseases “Lazzaro Spallanzani” IRCCS, 00149 Rome, Italy; (M.S.); (F.V.)
| | - Teresa Bossù
- Food Microbiology Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, 00178 Rome, Italy; (V.R.); (B.M.V.); (T.B.); (S.B.)
| | - Stefano Bilei
- Food Microbiology Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, 00178 Rome, Italy; (V.R.); (B.M.V.); (T.B.); (S.B.)
| | - Maria Laura De Marchis
- Food Microbiology Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, 00178 Rome, Italy; (V.R.); (B.M.V.); (T.B.); (S.B.)
| |
Collapse
|
|
32
|
Bland R, Brown SRB, Waite-Cusic J, Kovacevic J. Probing antimicrobial resistance and sanitizer tolerance themes and their implications for the food industry through the Listeria monocytogenes lens. Compr Rev Food Sci Food Saf 2022; 21:1777-1802. [PMID: 35212132 DOI: 10.1111/1541-4337.12910] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/21/2021] [Revised: 12/18/2021] [Accepted: 12/23/2021] [Indexed: 12/12/2022]
Abstract
The development of antibiotic resistance is a serious public health crisis, reducing our ability to effectively combat infectious bacterial diseases. The parallel study of reduced susceptibility to sanitizers is growing, particularly for environmental foodborne pathogens, such as Listeria monocytogenes. As regulations demand a seek-and-destroy approach for L. monocytogenes, understanding sanitizer efficacy and its uses are critical for the food industry. Studies have reported the ability of L. monocytogenes to survive in sanitizer concentrations 10-1000 times lower than the manufacturer-recommended concentration (MRC). Notably, data show that at MRC and when applied according to the label instructions, sanitizers remain largely effective. Studies also report that variables such as the presence of organic material, application time/temperature, and bacterial attachment to surfaces can impact sanitizer effectiveness. Due to the lack of standardization in the methodology and definitions of sanitizer resistance, tolerance, and susceptibility, different messages are conveyed in different studies. In this review, we examine the diversity of definitions, terminology, and methodologies used in studies examining L. monocytogenes resistance and susceptibility to antimicrobials. Research available to date fails to demonstrate "resistance" of L. monocytogenes to recommended sanitizer treatments as prescribed by the label. As such, sanitizer tolerance would be a more accurate description of L. monocytogenes response to low sanitizer concentrations (i.e., sub-MRC). Conservative use of word "resistance" will reduce confusion and allow for concise messaging as sanitizer research findings are communicated to industry and regulators.
Collapse
Affiliation(s)
- Rebecca Bland
- Food Innovation Center, Oregon State University, Portland, Oregon, USA.,Department of Food Science and Technology, Oregon State University, Corvallis, Oregon, USA
| | - Stephanie R B Brown
- Food Innovation Center, Oregon State University, Portland, Oregon, USA.,Department of Food Science and Technology, Oregon State University, Corvallis, Oregon, USA
| | - Joy Waite-Cusic
- Department of Food Science and Technology, Oregon State University, Corvallis, Oregon, USA
| | - Jovana Kovacevic
- Food Innovation Center, Oregon State University, Portland, Oregon, USA.,Department of Food Science and Technology, Oregon State University, Corvallis, Oregon, USA
| |
Collapse
|
|
33
|
Megli CJ, Coyne CB. Infections at the maternal-fetal interface: an overview of pathogenesis and defence. Nat Rev Microbiol 2022; 20:67-82. [PMID: 34433930 PMCID: PMC8386341 DOI: 10.1038/s41579-021-00610-y] [Citation(s) in RCA: 216] [Impact Index Per Article: 72.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 07/15/2021] [Indexed: 02/08/2023]
Abstract
Infections are a major threat to human reproductive health, and infections in pregnancy can cause prematurity or stillbirth, or can be vertically transmitted to the fetus leading to congenital infection and severe disease. The acronym 'TORCH' (Toxoplasma gondii, other, rubella virus, cytomegalovirus, herpes simplex virus) refers to pathogens directly associated with the development of congenital disease and includes diverse bacteria, viruses and parasites. The placenta restricts vertical transmission during pregnancy and has evolved robust mechanisms of microbial defence. However, microorganisms that cause congenital disease have likely evolved diverse mechanisms to bypass these defences. In this Review, we discuss how TORCH pathogens access the intra-amniotic space and overcome the placental defences that protect against microbial vertical transmission.
Collapse
Affiliation(s)
- Christina J Megli
- Division of Maternal-Fetal Medicine, Division of Reproductive Infectious Disease, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine and the Magee Womens Research Institute, Pittsburgh, PA, USA.
| | - Carolyn B Coyne
- Department of Molecular Genetics and Microbiology and the Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC, USA.
| |
Collapse
|
|
34
|
Schaefer K, Austhof E, Boyd K, Armstrong A, Hoffman S, Pogreba-Brown K. Septicemia Due to Listeria monocytogenes Infection: A Systematic Review and Meta-Analysis. Foodborne Pathog Dis 2021; 19:104-114. [PMID: 34883025 DOI: 10.1089/fpd.2021.0046] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/17/2022] Open
Abstract
Listeriosis is a rare bacterial infection associated with foodborne illness that can result in septicemia, a serious acute outcome. Sepsis is responsible for one in three deaths during hospitalization. The objective of this study was to conduct a systematic review and meta-analysis to estimate the proportion of Listeria monocytogenes infections resulting in septicemia. PubMed, Embase, Scopus, and Web of Science were searched from January 1, 2000, to April 1, 2018, for epidemiological studies that assessed studies focusing on L. monocytogenes infections with the outcome of septicemia. Articles in English, Spanish, and Portuguese using case-control, cohort, or outbreak studies reporting measures of association between L. monocytogenes and septicemia were included. Bias and heterogeneity were assessed using univariate meta-regression for region, sample size, study design, and report method. Nineteen articles were eligible for inclusion post-screening, the majority of which were conducted in Europe (n = 15); utilized a retrospective cohort design (n = 16); and collected data via routine or laboratory surveillance methods (n = 10). Prevalence of sepsis ranged from 4.2% to 100% among study populations of 6 to 1374 individuals. Overall, the proportion of listeriosis cases that developed sepsis was 46% (95% confidence interval [CI] 31.0-61.0%); for neonatal cases, 21.3% (95% CI 11.0-31.6%); and for maternal and neonatal cases, 18.8% (95% CI 10.7-26.8%). The heterogeneity was high for overall and group meta-analyses, but it could not be explained by the subanalyses for the overall proportion, whereas for neonatal, and neonatal and maternal cases combined, China had a significantly lower proportion than Europe and the United States. Septicemia following L. monocytogenes infection is a severe acute complication with 31-61% rate found overall; however, greater delineation of demographic data is needed to determine important risk factors. Future research should aim to address the gaps in knowledge in the long-term outcomes of sepsis from L. monocytogenes infection, and whether these outcomes differ from those due to other infections.
Collapse
Affiliation(s)
- Kenzie Schaefer
- Department of Epidemiology and Biostatistics, University of Arizona, Tucson, Arizona, USA
| | - Erika Austhof
- Department of Epidemiology and Biostatistics, University of Arizona, Tucson, Arizona, USA
| | - Kylie Boyd
- Department of Epidemiology and Biostatistics, University of Arizona, Tucson, Arizona, USA
| | - Alexandra Armstrong
- Department of Epidemiology and Biostatistics, University of Arizona, Tucson, Arizona, USA
| | - Sandra Hoffman
- U.S. Department of Agriculture, Economic Research Service, Washington, District of Columbia, USA
| | - Kristen Pogreba-Brown
- Department of Epidemiology and Biostatistics, University of Arizona, Tucson, Arizona, USA
| |
Collapse
|
|
35
|
Ntuli N, Wadula J, Nakwa F, Thomas R, Van Kwawegen A, Sepeng L, Seake K, Kgwadi D, Sono L, Ondongo-Ezhet C, Velaphi S. Characteristics and Outcomes of Neonates With Blood Stream Infection Due to Listeria monocytogenes. Pediatr Infect Dis J 2021; 40:917-921. [PMID: 34310508 DOI: 10.1097/inf.0000000000003213] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/25/2022]
Abstract
BACKGROUND Infection due to Listeria monocytogenes (LM) is rare in neonates; thus, its clinical presentation and outcomes are not commonly reported, especially in low- and middle-income countries. In 2017, South Africa had an outbreak due to LM. OBJECTIVE To determine demographic characteristics, clinical and laboratory findings and outcomes of all neonates infected with LM during the outbreak period. METHODS This is a retrospective analytic study. Clinical and laboratory records of neonates admitted at Chris Hani Baragwanath Academic Hospital from January 2017 to May 2018 with positive blood and cerebrospinal fluid culture with LM were reviewed for demographic characteristics, clinical presentation, ancillary laboratory test results and outcomes at hospital discharge. RESULTS There were 42 neonates with positive cultures due to LM. Thirty-four (81%) were born preterm. Mode of delivery was vaginal in 78.6% and 31.0% were HIV exposed. All patients presented within the first 6 days of life as an early-onset disease. Common clinical presentation was respiratory depression (52.4%) and respiratory distress (38.1%) with 69% requiring invasive or noninvasive respiratory support. Common abnormal laboratory findings were high C-reactive protein (77.1%) followed by leukopenia (23.8%). Fourteen patients (40%) had features of meningitis based on blood and cerebrospinal fluid findings (4 culture proven). There were 11 deaths at hospital discharge, giving a mortality rate of 26.2%. CONCLUSIONS The majority of neonates infected with LM were born preterm, raising the possibility that LM itself may have been responsible for preterm labor. All presented in the first 6 days of life and most presented with respiratory distress or depression. A high proportion had meningitis, and there was a high-mortality overall.
Collapse
Affiliation(s)
- Nandi Ntuli
- From the School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
| | - Jeannette Wadula
- Department of Clinical Microbiology and Infectious Diseases, School of Pathology, Faculty of Health Sciences, Chris Hani Baragwanath Academic Hospital, University of the Witwatersrand and National Health Laboratory Services, Johannesburg, South Africa
| | - Firdose Nakwa
- From the School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
| | - Reenu Thomas
- From the School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
| | - Alison Van Kwawegen
- From the School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
| | - Letlhogonolo Sepeng
- From the School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
| | - Karabo Seake
- From the School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
| | - Dikeledi Kgwadi
- From the School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
| | - Lino Sono
- From the School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
| | - Claude Ondongo-Ezhet
- From the School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
| | - Sithembiso Velaphi
- From the School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
| |
Collapse
|
|
36
|
Ciaranello AL, Tran KM, Audin CR, Anahtar MN. Case 21-2021: A 33-Year-Old Pregnant Woman with Fever, Abdominal Pain, and Headache. N Engl J Med 2021; 385:265-274. [PMID: 34260840 DOI: 10.1056/nejmcpc2107344] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/19/2022]
Affiliation(s)
- Andrea L Ciaranello
- From the Departments of Medicine (A.L.C., K.M.T.), Radiology (C.R.A.), and Pathology (M.N.A.), Massachusetts General Hospital, and the Departments of Medicine (A.L.C., K.M.T.), Radiology (C.R.A.), and Pathology (M.N.A.), Harvard Medical School - both in Boston
| | - Kathy M Tran
- From the Departments of Medicine (A.L.C., K.M.T.), Radiology (C.R.A.), and Pathology (M.N.A.), Massachusetts General Hospital, and the Departments of Medicine (A.L.C., K.M.T.), Radiology (C.R.A.), and Pathology (M.N.A.), Harvard Medical School - both in Boston
| | - Craig R Audin
- From the Departments of Medicine (A.L.C., K.M.T.), Radiology (C.R.A.), and Pathology (M.N.A.), Massachusetts General Hospital, and the Departments of Medicine (A.L.C., K.M.T.), Radiology (C.R.A.), and Pathology (M.N.A.), Harvard Medical School - both in Boston
| | - Melis N Anahtar
- From the Departments of Medicine (A.L.C., K.M.T.), Radiology (C.R.A.), and Pathology (M.N.A.), Massachusetts General Hospital, and the Departments of Medicine (A.L.C., K.M.T.), Radiology (C.R.A.), and Pathology (M.N.A.), Harvard Medical School - both in Boston
| |
Collapse
|
|
37
|
Listeria Rhomboencephalitis in an Immunocompetent Host. INFECTIOUS DISEASES IN CLINICAL PRACTICE 2021. [DOI: 10.1097/ipc.0000000000001024] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/25/2022]
|
|
38
|
Yousefi A, Karbalaei M, Keikha M. Extraintestinal infection of Listeria monocytogenes and susceptibility to spontaneous abortion during pregnancy: A systematic review and meta-analysis. World J Meta-Anal 2021; 9:317-326. [DOI: 10.13105/wjma.v9.i3.317] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 04/23/2021] [Revised: 06/13/2021] [Accepted: 06/16/2021] [Indexed: 02/06/2023] Open
|
|
39
|
Wang Z, Tao X, Liu S, Zhao Y, Yang X. An Update Review on Listeria Infection in Pregnancy. Infect Drug Resist 2021; 14:1967-1978. [PMID: 34079306 PMCID: PMC8165209 DOI: 10.2147/idr.s313675] [Citation(s) in RCA: 36] [Impact Index Per Article: 9.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/31/2021] [Accepted: 05/18/2021] [Indexed: 12/31/2022] Open
Abstract
Listeria monocytogenes (LM) is an intracellular, aerobic and facultative anaerobic, Gram-positive bacterium, which is primarily transmitted to humans orally via food. LM could occur in asymptomatic pregnant women; however, fetal infection is a serious condition, entailing premature birth, abortion, sepsis, central nervous system (CNS) involvement, or even death. If a pregnant woman exhibits symptoms, the performance is almost like influenza, such as fever, headache, diarrhea, myalgia, or other digestive-related symptoms. This review collected clinical and empirical results regarding the mechanism, clinical manifestations, obstetrical outcome, diagnosis, treatment, vertical transmission, neonatal infection, and prevention of listeriosi according to articles published in PubMed from January 1, 1980, to March 20, 2021. The early detection and diagnosis of pregnancy-associated listeriosis are significant since sensitive antibiotics are effective at enhancing the prognosis of newborns. Listeriosis can be diagnosed using positive cultures from maternal or neonatal blood, neonatal cerebrospinal fluid (CSF), amniotic fluid, intrauterine mucosa, or the placenta. Two weeks of high-dose intravenous amoxicillin (more than 6 g/day) is recommended for LM pregnant women without allergy. Terminating the pregnancy to save the mother’s life should be considered if maternal and fetal conditions aggravate. Neonatal Listeria infection is primarily transmitted through the placenta, which is a critical illness associated with a high mortality rate. The necessary dietary guidance for pregnant women can reduce the incidence rate of pregnancy-related listeriosis.
Collapse
Affiliation(s)
- Zhaoyun Wang
- Department of Clinical Medicine, China Medical University, Shenyang, Liaoning, People's Republic of China
| | - Xiaojing Tao
- Department of Clinical Medicine, China Medical University, Shenyang, Liaoning, People's Republic of China
| | - Shan Liu
- Department of Clinical Medicine, China Medical University, Shenyang, Liaoning, People's Republic of China
| | - Yutong Zhao
- Department of Clinical Medicine, China Medical University, Shenyang, Liaoning, People's Republic of China
| | - Xiuhua Yang
- Department of Obstetrics, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China
| |
Collapse
|
|
40
|
Charlier C, Kermorvant-Duchemin E, Perrodeau E, Moura A, Maury MM, Bracq-Dieye H, Thouvenot P, Valès G, Leclercq A, Ravaud P, Lecuit M. Neonatal listeriosis presentation and outcome: a prospective study of 189 cases. Clin Infect Dis 2021; 74:8-16. [PMID: 33876229 DOI: 10.1093/cid/ciab337] [Citation(s) in RCA: 18] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/22/2021] [Indexed: 11/14/2022] Open
Abstract
CONTEXT Listeriosis is caused by the foodborne pathogen Listeria monocytogenes. It can present as a maternal-neonatal infection. We implemented the nationwide prospective cohort MONALISA and analyzed the features of neonatal listeriosis. METHODS We studied all neonates born alive from mothers with microbiologically-proven maternal-neonatal listeriosis enrolled from November 2009 to December 2017. We analyzed presentation, neonatal outcome at discharge and predictors of severe presentation and outcome. The study is registered at clinicaltrials.gov (NCT01520597). RESULTS We studied 189 infants. 133/189 (70%) had abnormal clinical status at birth, including acute respiratory distress in 106/189 (56%). 132/189 (70%) infants developed early-onset listeriosis and 12/189 (6%) late onset listeriosis who all presented with acute meningitis. 17/189 (9%) had major adverse outcomes: 3% death (5/189), 6% (12/189) severe brain injury, 2% (3/189) severe bronchopulmonary dysplasia, 15/17 in infants born < 34 weeks of gestation (p < 0.0001 versus infants born ≥ 34 weeks of gestation). Maternal antimicrobial treatment ≥ 1 day before delivery was associated with a significant decrease of infants' severity (resulting in significantly less inotropic drugs, fluid resuscitation, or mechanical ventilation requirement), OR 0.23 [95% confidence interval CI 0.09-0.51], p < 0.0001). CONCLUSION Antenatal maternal antimicrobial treatment is associated with reduced neonatal listeriosis severity, justifying the prescription of preemptive maternal antimicrobial therapy when maternal-fetal listeriosis is suspected. Neonatal outcome is better than reported earlier, and its major determinant is gestational age at birth.
Collapse
Affiliation(s)
- Caroline Charlier
- Institut Pasteur, Biology of Infection Unit, Paris, France.,Institut Pasteur, French National Reference Center and WHO Collaborating Center Listeria, Paris, France.,Inserm U1117, Paris, France.,Université de Paris, Paris, France.,Necker-Enfants Malades University Hospital, Department of Infectious Diseases and Tropical Medicine, Institut Imagine, AP-HP, Paris, France
| | - Elsa Kermorvant-Duchemin
- Université de Paris, Paris, France.,Necker-Enfants Malades University Hospital, Department of Neonatology, AP-HP, Paris, France
| | - Elodie Perrodeau
- Centre of Research in Epidemiology and Statistics Sorbonne Paris Cité, METHODS Team, UMR 1153, Inserm, Université de Paris, Paris, France
| | - Alexandra Moura
- Institut Pasteur, Biology of Infection Unit, Paris, France.,Institut Pasteur, French National Reference Center and WHO Collaborating Center Listeria, Paris, France.,Inserm U1117, Paris, France
| | - Mylène M Maury
- Institut Pasteur, Biology of Infection Unit, Paris, France.,Institut Pasteur, French National Reference Center and WHO Collaborating Center Listeria, Paris, France.,Inserm U1117, Paris, France
| | - Hélène Bracq-Dieye
- Institut Pasteur, French National Reference Center and WHO Collaborating Center Listeria, Paris, France
| | - Pierre Thouvenot
- Institut Pasteur, French National Reference Center and WHO Collaborating Center Listeria, Paris, France
| | - Guillaume Valès
- Institut Pasteur, French National Reference Center and WHO Collaborating Center Listeria, Paris, France
| | - Alexandre Leclercq
- Institut Pasteur, French National Reference Center and WHO Collaborating Center Listeria, Paris, France
| | - Philippe Ravaud
- Université de Paris, Paris, France.,Centre of Research in Epidemiology and Statistics Sorbonne Paris Cité, METHODS Team, UMR 1153, Inserm, Université de Paris, Paris, France
| | - Marc Lecuit
- Institut Pasteur, Biology of Infection Unit, Paris, France.,Institut Pasteur, French National Reference Center and WHO Collaborating Center Listeria, Paris, France.,Inserm U1117, Paris, France.,Université de Paris, Paris, France.,Necker-Enfants Malades University Hospital, Department of Infectious Diseases and Tropical Medicine, Institut Imagine, AP-HP, Paris, France
| | | |
Collapse
|
|
41
|
Lu B, Yang J, Gao C, Li D, Cui Y, Huang L, Chen X, Wang D, Wang A, Liu Y, Li Y, Zhang Z, Jiao M, Xu H, Song Y, Fu B, Xu L, Yang Q, Ning Y, Wang L, Bao C, Luo G, Wu H, Yang T, Li C, Tang M, Wang J, Guo W, Zeng J, Zhong W. Listeriosis Cases and Genetic Diversity of Their L. monocytogenes Isolates in China, 2008-2019. Front Cell Infect Microbiol 2021; 11:608352. [PMID: 33680989 PMCID: PMC7933659 DOI: 10.3389/fcimb.2021.608352] [Citation(s) in RCA: 19] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/28/2020] [Accepted: 01/04/2021] [Indexed: 12/12/2022] Open
Abstract
Listeriosis, caused by Listeria monocytogenes, is a severe food-borne infection. The nationwide surveillance in China concerning listeriosis is urgently needed. In the present study, 144 L. monocytogenes isolates were collected from the samples of blood, cerebrospinal fluid (CSF), and fetal membrane/placenta in China for 12 years from 2008 to 2019. We summarized these listeriosis patients’ demographical and clinical features and outcomes. The susceptibility profile for 12 antibiotics was also determined by the broth microdilution method. Multilocus sequence typing (MLST) and serogroups of these listeria isolates were analyzed to designate epidemiological types. We enrolled 144 cases from 29 healthcare centers, including 96 maternal-neonatal infections, 33 cases of bacteremia, 13 cases of neurolisteriosis, and two cutaneous listeriosis. There were 31 (59.6%) fetal loss in 52 pregnant women and four (9.8%) neonatal death in 41 newborns. Among the 48 nonmaternal-neonatal cases, 12.5% (6/48) died, 41.7% (20/48) were female, and 64.6% (31/48) occurred in those with significant comorbidities. By MLST, the strains were distinguished into 23 individual sequence types (STs). The most prevalent ST was ST87 (49 isolates, 34.0%), followed by ST1 (18, 12.5%), ST8 (10, 6.9%), ST619 (9, 6.3%), ST7 (7, 4.9%) and ST3 (7, 4.9%). Furthermore, all L. monocytogenes isolates were uniformly susceptible to penicillin, ampicillin, and meropenem. In summary, our study highlights a high genotypic diversity of L. monocytogenes strains causing clinical listeriosis in China. Furthermore, a high prevalence of ST87 and ST1 in the listeriosis should be noted.
Collapse
Affiliation(s)
- Binghuai Lu
- Laboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing, China.,Center for Respiratory Diseases, China-Japan Friendship Hospital, Beijing, China.,Laboratory of Clinical Microbiology and Infectious Diseases, National Clinical Research Center of Respiratory Diseases, Beijing, China
| | - Junwen Yang
- Department of Laboratory Medicine, Zhengzhou Key Laboratory of Children's Infection and Immunity, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou, China
| | - Chunyan Gao
- Department of Laboratory Medicine, Tangshan Maternal and Child Health Care Hospital, Tangshan, China
| | - Dong Li
- Department of Laboratory Medicine, Civil Aviation General Hospital, Beijing, China
| | - Yanchao Cui
- Department of Laboratory Medicine, Civil Aviation General Hospital, Beijing, China
| | - Lei Huang
- Department of Laboratory Medicine, Peking University First Hospital, Beijing, China
| | - Xingchun Chen
- Department of Laboratory Medicine, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China
| | - Duochun Wang
- National Institute for Communicable Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Chinese Centre for Disease Control and Prevention, Beijing, China
| | - Aiping Wang
- Department of Laboratory Medicine, Beijing Anzhen Hospital, Beijing, China
| | - Yulei Liu
- Department of Laboratory Medicine, Beijing Anzhen Hospital, Beijing, China
| | - Yi Li
- Department of Laboratory Medicine, Henan Provincial People's Hospital, Zhengzhou, China
| | - Zhijun Zhang
- Department of Laboratory Medicine, Tai'an City Central Hospital, Tai'an, China
| | - Mingyuan Jiao
- Department of Laboratory Medicine, Beijing Tongzhou District Maternal and Child Healthcare Hospital, Beijing, China
| | - Heping Xu
- Department of Laboratory Medicine, First Affiliated Hospital of Xiamen University, Xiamen, China
| | - Yu Song
- Department of Laboratory Medicine, Daqing Oilfield General Hospital, Daqing, China
| | - Baoqing Fu
- Department of Laboratory Medicine, Daqing Oilfield General Hospital, Daqing, China
| | - Lili Xu
- Department of Laboratory Medicine, Fifth People's Hospital of Chengdu, Chengdu, China
| | - Qing Yang
- Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
| | - Yongzhong Ning
- Department of Laboratory Medicine, Chui Yang Liu Hospital Affiliated to Tsinghua University, Beijing, China
| | - Lijun Wang
- Department of Laboratory Medicine, Beijing Tsinghua Chang Gung Hospital, Tsinghua University, Beijing, China
| | - Chunmei Bao
- Clinical Laboratory Medical Center, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
| | - Guolan Luo
- Department of Laboratory Medicine, Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, China
| | - Hua Wu
- Department of Laboratory Medicine, Hainan General Hospital, Haikou, China
| | - Tongshu Yang
- Department of Laboratory Medicine, The Affiliated Tumor Hospital of Harrbin Medical University, Harbin, China
| | - Chen Li
- Department of Laboratory Medicine, Liuyang City Traditional Chinese Medicine Hospital, Liuyang, China
| | - Manjuan Tang
- Department of Laboratory Medicine, Xiangtan Central Hospital, Xiangtan, China
| | - Junrui Wang
- Department of Laboratory Medicine, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China
| | - Wenchen Guo
- Department of Laboratory Medicine, Weifang People's Hospital, Weifang, China
| | - Ji Zeng
- Department of Laboratory Medicine, Wuhan Pu Ai Hospital of Huazhong University of Science and Technology, Wuhan, China
| | - Wen Zhong
- Department of Laboratory Medicine, Ningde Hospital, Fujian Medical University, Ningde, China
| |
Collapse
|
|
42
|
Maternal Microbiome and Infections in Pregnancy. Microorganisms 2020; 8:microorganisms8121996. [PMID: 33333813 PMCID: PMC7765218 DOI: 10.3390/microorganisms8121996] [Citation(s) in RCA: 46] [Impact Index Per Article: 9.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/08/2020] [Revised: 12/09/2020] [Accepted: 12/13/2020] [Indexed: 12/14/2022] Open
Abstract
Pregnancy induces unique changes in maternal immune responses and metabolism. Drastic physiologic adaptations, in an intricately coordinated fashion, allow the maternal body to support the healthy growth of the fetus. The gut microbiome plays a central role in the regulation of the immune system, metabolism, and resistance to infections. Studies have reported changes in the maternal microbiome in the gut, vagina, and oral cavity during pregnancy; it remains unclear whether/how these changes might be related to maternal immune responses, metabolism, and susceptibility to infections during pregnancy. Our understanding of the concerted adaption of these different aspects of the human physiology to promote a successful pregnant remains limited. Here, we provide a comprehensive documentation and discussion of changes in the maternal microbiome in the gut, oral cavity, and vagina during pregnancy, metabolic changes and complications in the mother and newborn that may be, in part, driven by maternal gut dysbiosis, and, lastly, common infections in pregnancy. This review aims to shed light on how dysregulation of the maternal microbiome may underlie obstetrical metabolic complications and infections.
Collapse
|
|
43
|
Charlier C, Disson O, Lecuit M. Maternal-neonatal listeriosis. Virulence 2020; 11:391-397. [PMID: 32363991 PMCID: PMC7199740 DOI: 10.1080/21505594.2020.1759287] [Citation(s) in RCA: 47] [Impact Index Per Article: 9.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/08/2019] [Revised: 03/14/2020] [Accepted: 03/29/2020] [Indexed: 02/06/2023] Open
Abstract
Listeriosis is a rare and severe foodborne infection caused by Listeria monocytogenes. It manifests as septicemia, neurolisteriosis, and maternal-fetal infection. In pregnancy, it may cause maternal fever, premature delivery, fetal loss, neonatal systemic and central nervous system infections. Maternal listeriosis is mostly reported during the 2nd and 3rd trimester of pregnancy, as sporadic cases or in the context of outbreaks. Strains belonging to clonal complexes 1, 4 and 6, referred to as hypervirulent, are the most associated to maternal-neonatal infections. Here we review the clinical, pathophysiological, and microbiological features of maternal-neonatal listeriosis.
Collapse
Affiliation(s)
- Caroline Charlier
- Institut Pasteur, Biology of Infection Unit, Paris, France
- Inserm U1117, Paris, France
- Institut Pasteur, French National Reference Center and WHO Collaborating Center for Listeria, Paris, France
- Hôpital Universitaire Necker-Enfants Malades, Service des Maladies Infectieuses et Tropicales, Institut Imagine, APHP, Paris, France
- Université de Paris, Paris, France
| | - Olivier Disson
- Institut Pasteur, Biology of Infection Unit, Paris, France
- Inserm U1117, Paris, France
| | - Marc Lecuit
- Institut Pasteur, Biology of Infection Unit, Paris, France
- Inserm U1117, Paris, France
- Institut Pasteur, French National Reference Center and WHO Collaborating Center for Listeria, Paris, France
- Hôpital Universitaire Necker-Enfants Malades, Service des Maladies Infectieuses et Tropicales, Institut Imagine, APHP, Paris, France
- Université de Paris, Paris, France
| |
Collapse
|
|
44
|
Zbinden FR, De Ste Croix M, Grandgirard D, Haigh RD, Vacca I, Zamudio R, Goodall ECA, Stephan R, Oggioni MR, Leib SL. Pathogenic Differences of Type 1 Restriction-Modification Allele Variants in Experimental Listeria monocytogenes Meningitis. Front Cell Infect Microbiol 2020; 10:590657. [PMID: 33194838 PMCID: PMC7662400 DOI: 10.3389/fcimb.2020.590657] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/02/2020] [Accepted: 09/25/2020] [Indexed: 11/13/2022] Open
Abstract
Background: L. monocytogenes meningoencephalitis has a mortality rate of up to 50% and neurofunctional sequelae are common. Type I restriction-modification systems (RMS) are capable of adding methyl groups to the host genome. Some contain multiple sequence recognition (hsdS) genes that recombine, resulting in distinct DNA methylation patterns and patterns of gene expression. These phenotypic switches have been linked to virulence and have recently been discovered in multiple clonal complexes of L. monocytogenes. In the present study, we investigated the significant of RMS on L. monocytogenes virulence during the acute phase of experimental meningitis. Methods: L. monocytogenes strains containing RMS systems were identified, and purified clones enriched for single hsdS alleles were isolated. In vivo, 11-day old Wistar rats were infected with an inoculum containing (a) one of 4 single RMS allele variants (A, B, C, D) treated with amoxicillin (AMX 50 mg/kg/dosis, q8h), (b) a mixture of all 4 variants with or without AMX treatment, or (c) different mixtures of 2 RMS allele variants. At selected time points after infection, clinical and inflammatory parameters, bacterial titers and brain damage were determined. Changes in the relative frequency of the occurring RMS alleles in the inoculum and in CSF or cerebellum of infected animals were analyzed by capillary electrophoresis. Results: We have identified a phase variable RMS locus within L. monocytogenes CC4 and generated stocks that stably expressed each of the possible hsdS genes within that loci. Generation of these allele variants (A, B, C, D) allowed us to determine the methylation pattern associated with each hsdS through SMRT sequencing. In vivo infections with these single allele variants revealed differences in disease severity in that C induced the worst clinical outcome and more pronounced hippocampal apoptosis; D showed the most pronounced weight loss and the highest bacterial titer in the cerebellum. A caused the least severe disease. Conclusion: We identified that L. monocytogenes expressing hsdS (A) causes less damage than when other hsdS genes are expressed. While expression of hsdSC and D worsened the outcome in L. monocytogenes meningitis. We also demonstrate a competitive advantage of variants C and B over variant A in this model. Phenotypical switching may therefore represent a mechanism of virulence regulation during the acute phase of CNS infections with L. monocytogenes.
Collapse
Affiliation(s)
- Florian R Zbinden
- Neuroinfection Laboratory, Institute for Infectious Diseases, University of Bern, Bern, Switzerland
| | - Megan De Ste Croix
- Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom
| | - Denis Grandgirard
- Neuroinfection Laboratory, Institute for Infectious Diseases, University of Bern, Bern, Switzerland
| | - Richard D Haigh
- Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom
| | - Irene Vacca
- Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom
| | - Roxana Zamudio
- Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom
| | - Emily C A Goodall
- Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom
| | - Roger Stephan
- Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland
| | - Marco R Oggioni
- Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom
| | - Stephen L Leib
- Neuroinfection Laboratory, Institute for Infectious Diseases, University of Bern, Bern, Switzerland
| |
Collapse
|
|
45
|
Abu-Raya B, Michalski C, Sadarangani M, Lavoie PM. Maternal Immunological Adaptation During Normal Pregnancy. Front Immunol 2020; 11:575197. [PMID: 33133091 PMCID: PMC7579415 DOI: 10.3389/fimmu.2020.575197] [Citation(s) in RCA: 332] [Impact Index Per Article: 66.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/22/2020] [Accepted: 09/18/2020] [Indexed: 12/25/2022] Open
Abstract
The risk and severity of specific infections are increased during pregnancy due to a combination of physiological and immunological changes. Characterizing the maternal immune system during pregnancy is important to understand how the maternal immune system maintains tolerance towards the allogeneic fetus. This may also inform strategies to prevent maternal fatalities due to infections and optimize maternal vaccination to best protect the mother-fetus dyad and the infant after birth. In this review, we describe what is known about the immunological changes that occur during a normal pregnancy.
Collapse
Affiliation(s)
- Bahaa Abu-Raya
- Vaccine Evaluation Center, BC Children's Hospital Research Institute, Vancouver, BC, Canada.,BC Children's Hospital Research Institute, Vancouver, BC, Canada.,Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada.,Experimental Medicine Program, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada
| | - Christina Michalski
- BC Children's Hospital Research Institute, Vancouver, BC, Canada.,Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada.,Experimental Medicine Program, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada
| | - Manish Sadarangani
- Vaccine Evaluation Center, BC Children's Hospital Research Institute, Vancouver, BC, Canada.,BC Children's Hospital Research Institute, Vancouver, BC, Canada.,Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada.,Experimental Medicine Program, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada
| | - Pascal M Lavoie
- BC Children's Hospital Research Institute, Vancouver, BC, Canada.,Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada.,Experimental Medicine Program, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada
| |
Collapse
|
|
46
|
|
|
47
|
Eissa S, Zourob M. Ultrasensitive peptide-based multiplexed electrochemical biosensor for the simultaneous detection of Listeria monocytogenes and Staphylococcus aureus. Mikrochim Acta 2020; 187:486. [PMID: 32761391 DOI: 10.1007/s00604-020-04423-3] [Citation(s) in RCA: 49] [Impact Index Per Article: 9.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/30/2020] [Accepted: 06/30/2020] [Indexed: 01/09/2023]
Abstract
A novel electrochemical biosensor is reported for simultaneous detection of two of the most common food-borne pathogens: Listeria monocytogenes and Staphylococcus aureus. The biosensor is composed of an array of gold nanoparticles-modified screen-printed carbon electrodes on which magnetic nanoparticles coupled to specific peptides were immobilized via streptavidin-biotin interaction. Taking advantage of the proteolytic activities of the protease enzymes produced from the two bacteria on the specific peptides, the detection was achieved in 1 min. The detection was realized by measuring the percentage increase of the square wave voltammetric peak current at 0.1 V versus a Ag/AgCl reference electrode in ferro/ferricyanide redox couple after incubation with the bacteria protease. The integration of the specificity of the bacterial enzymes towards their peptide substrates with the sensitivity of the electrochemical detection on the sensor array allows the rapid, sensitive and selective quantification of the two bacteria. Outstanding sensitivities were achieved using this biosensor array platform with limit of detection of 9 CFU mL-1 for Listeria monocytogenes and 3 CFU mL-1 for Staphylococcus aureus. The multiplexing capability and selectivity of the array voltammetric biosensor were demonstrated by analysing samples of Staphylococcus aureus, Listeria monocytogenes or E. coli and also containing a mixture of two or three bacteria. Using this biosensor, the two bacteria were successfully quantified simultaneously in one step without the need for DNA extraction or amplification techniques. This platform offers promise for rapid, simple and cost-effective simultaneous detection of various bacteria. Graphical abstract.
Collapse
Affiliation(s)
- Shimaa Eissa
- Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Road, Riyadh, 11533, Saudi Arabia
| | - Mohammed Zourob
- Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Road, Riyadh, 11533, Saudi Arabia. .,King Faisal Specialist Hospital and Research Center, Zahrawi Street, Al Maather, Riyadh, 12713, Saudi Arabia.
| |
Collapse
|
|
48
|
Phelps CC, Vadia S, Boyaka PN, Varikuti S, Attia Z, Dubey P, Satoskar AR, Tweten R, Seveau S. A listeriolysin O subunit vaccine is protective against Listeria monocytogenes. Vaccine 2020; 38:5803-5813. [PMID: 32684498 DOI: 10.1016/j.vaccine.2020.06.049] [Citation(s) in RCA: 17] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/08/2019] [Revised: 05/12/2020] [Accepted: 06/18/2020] [Indexed: 12/15/2022]
Abstract
Listeria monocytogenes is a facultative intracellular pathogen responsible for the life-threatening disease listeriosis. The pore-forming toxin listeriolysin O (LLO) is a critical virulence factor that plays a major role in the L. monocytogenes intracellular lifecycle and is indispensable for pathogenesis. LLO is also a dominant antigen for T cells involved in sterilizing immunity and it was proposed that LLO acts as a T cell adjuvant. In this work, we generated a novel full-length LLO toxoid (LLOT) in which the cholesterol-recognition motif, a threonine-leucine pair located at the tip of the LLO C-terminal domain, was substituted with two glycine residues. We showed that LLOT lost its ability to bind cholesterol and to form pores. Importantly, LLOT retained binding to the surface of epithelial cells and macrophages, suggesting that it could efficiently be captured by antigen-presenting cells. We then determined if LLOT can be used as an antigen and adjuvant to protect mice from L. monocytogenes infection. Mice were immunized with LLOT alone or together with cholera toxin or Alum as adjuvants. We found that mice immunized with LLOT alone or in combination with the Th2-inducing adjuvant Alum were not protected against L. monocytogenes. On the other hand, mice immunized with LLOT along with the experimental adjuvant cholera toxin, were protected against L. monocytogenes, as evidenced by a significant decrease in bacterial burden in the liver and spleen three days post-infection. This immunization regimen elicited mixed Th1, Th2, and Th17 responses, as well as the generation of LLO-neutralizing antibodies. Further, we identified T cells as being required for immunization-induced reductions in bacterial burden, whereas B cells were dispensable in our model of non-pregnant young mice. Overall, this work establishes that LLOT is a promising vaccine antigen for the induction of protective immunity against L. monocytogenes by subunit vaccines containing Th1-driving adjuvants.
Collapse
Affiliation(s)
- Christopher C Phelps
- Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA; Department of Microbiology, The Ohio State University, The Ohio State University, Columbus, OH, USA
| | - Stephen Vadia
- Department of Microbiology, The Ohio State University, The Ohio State University, Columbus, OH, USA; Department of Biology, Washington University in St. Louis, MO 63130, USA
| | - Prosper N Boyaka
- Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA; Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, USA
| | - Sanjay Varikuti
- Department of Pathology, The Ohio State University, Columbus, OH, USA
| | - Zayed Attia
- Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, USA
| | - Purnima Dubey
- Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA
| | - Abhay R Satoskar
- Department of Pathology, The Ohio State University, Columbus, OH, USA
| | - Rodney Tweten
- Department of Microbial & Immunology, University of Oklahoma, Oklahoma City, OK, USA
| | - Stephanie Seveau
- Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA; Department of Microbiology, The Ohio State University, The Ohio State University, Columbus, OH, USA.
| |
Collapse
|
|
49
|
Serventi L, Curi B, Johns R, Silva J, Bainbridge R, Gaither K. Pregnancy Complicated by Listeria Monocytogenes: A Case Report and Review of the Literature. J Natl Med Assoc 2020; 112:428-432. [PMID: 33526229 DOI: 10.1016/j.jnma.2020.05.002] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/19/2020] [Revised: 03/27/2020] [Accepted: 05/02/2020] [Indexed: 11/18/2022]
Abstract
Listeria Monocytogenes, a small facultative anaerobic, gram positive, motile bacillus is a rare, but consequential etiologic agent of food borne illness which inordinately impacts immunocompromised individuals. The organism infects many types of animals and contaminates a multitude of foodstuffs such as milk, chicken, beef and vegetables. This microbe additionally has a distinct proclivity to infect the maternal-fetoplacental unit with resultant adverse perinatal outcomes inclusive of spontaneous abortion, preterm delivery, chorioamnionitis, neonatal meningitis and death. We present a case of Listeriosis complicating pregnancy with a subsequent comprehensive review of the literature.
Collapse
Affiliation(s)
- Lisa Serventi
- NYC Health+ Hospitals/Lincoln, Department of Ob/Gyn, 234 East 149th Street, Bronx, 10451, NY
| | - Berenice Curi
- NYC Health+ Hospitals/Lincoln, Department of Ob/Gyn, 234 East 149th Street, Bronx, 10451, NY
| | - Rochelle Johns
- NYC Health+ Hospitals/Lincoln, Department of Ob/Gyn, 234 East 149th Street, Bronx, 10451, NY
| | - Jessica Silva
- NYC Health+ Hospitals/Lincoln, Department of Ob/Gyn, 234 East 149th Street, Bronx, 10451, NY
| | - Ronald Bainbridge
- NYC Health+ Hospitals/Lincoln, Department of Ob/Gyn, 234 East 149th Street, Bronx, 10451, NY
| | - Kecia Gaither
- NYC Health+ Hospitals/Lincoln, Department of Ob/Gyn, 234 East 149th Street, Bronx, 10451, NY.
| |
Collapse
|
|
50
|
Yu PA, Tran EL, Parker CM, Kim HJ, Yee EL, Smith PW, Russell Z, Nelson CA, Broussard CS, Yu YC, Meaney-Delman D. Safety of Antimicrobials During Pregnancy: A Systematic Review of Antimicrobials Considered for Treatment and Postexposure Prophylaxis of Plague. Clin Infect Dis 2020; 70:S37-S50. [PMID: 32435799 PMCID: PMC10867625 DOI: 10.1093/cid/ciz1231] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/31/2023] Open
Abstract
BACKGROUND The safety profile of antimicrobials used during pregnancy is one important consideration in the decision on how to treat and provide postexposure prophylaxis (PEP) for plague during pregnancy. METHODS We searched 5 scientific literature databases for primary sources on the safety of 9 antimicrobials considered for plague during pregnancy (amikacin, gentamicin, plazomicin, streptomycin, tobramycin, chloramphenicol, doxycycline, sulfadiazine, and trimethoprim-sulfamethoxazole [TMP-SMX]) and abstracted data on maternal, pregnancy, and fetal/neonatal outcomes. RESULTS Of 13 052 articles identified, 66 studies (case-control, case series, cohort, and randomized studies) and 96 case reports were included, totaling 27 751 prenatal exposures to amikacin (n = 9), gentamicin (n = 345), plazomicin (n = 0), streptomycin (n = 285), tobramycin (n = 43), chloramphenicol (n = 246), doxycycline (n = 2351), sulfadiazine (n = 870), and TMP-SMX (n = 23 602). Hearing or vestibular deficits were reported in 18/121 (15%) children and 17/109 (16%) pregnant women following prenatal streptomycin exposure. First trimester chloramphenicol exposure was associated with an elevated risk of an undescended testis (odds ratio [OR] 5.9, 95% confidence interval [CI] 1.2-28.7). Doxycycline was associated with cardiovascular malformations (OR 2.4, 95% CI 1.2-4.7) in 1 study and spontaneous abortion (OR 2.8, 95% CI 1.9-4.1) in a separate study. First trimester exposure to TMP-SMX was associated with increased risk of neural tube defects (pooled OR 2.5, 95% CI 1.4-4.3), spontaneous abortion (OR 3.5, 95% CI 2.3-5.6), preterm birth (OR 1.5, 95% CI 1.1-2.1), and small for gestational age (OR 1.6, 95% CI 1.2-2.2). No other statistically significant associations were reported. CONCLUSIONS For most antimicrobials reviewed, adverse maternal/fetal/neonatal outcomes were not observed consistently. Prenatal exposure to streptomycin and TMP-SMX was associated with select birth defects in some studies. Based on limited data, chloramphenicol and doxycycline may be associated with adverse pregnancy or neonatal outcomes; however, more data are needed to confirm these associations. Antimicrobials should be used for treatment and PEP of plague during pregnancy; the choice of antimicrobials may be influenced by these data as well as information about the risks of plague during pregnancy.
Collapse
Affiliation(s)
- Patricia A Yu
- Division of Preparedness and Emerging Infections, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA
| | - Emmy L Tran
- Division of Birth Defects and Infant Disorders, National Center on Birth Defects and Developmental Disabilities, CDC, Atlanta, Georgia, USA
- Eagle Global Scientific, LLC, San Antonio, Texas, USA
| | - Corinne M Parker
- Division of Preparedness and Emerging Infections, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA
- Chenega Professional and Technical Services, LLC, Atlanta, Georgia, USA
| | - Hye-Joo Kim
- Division of Preparedness and Emerging Infections, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA
- Chenega Professional and Technical Services, LLC, Atlanta, Georgia, USA
| | - Eileen L Yee
- Division of Preparedness and Emerging Infections, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA
- Chenega Professional and Technical Services, LLC, Atlanta, Georgia, USA
| | | | - Zachary Russell
- Division of Preparedness and Emerging Infections, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA
- Oak Ridge Institute for Science and Education CDC Fellowship Program, Atlanta, Georgia, USA
| | - Christina A Nelson
- Division of Vector-Borne Diseases, NCEZID, CDC, Fort Collins, Colorado, USA
| | - Cheryl S Broussard
- Division of Birth Defects and Infant Disorders, National Center on Birth Defects and Developmental Disabilities, CDC, Atlanta, Georgia, USA
| | - Yon C Yu
- Division of Preparedness and Emerging Infections, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA
| | - Dana Meaney-Delman
- Division of Birth Defects and Infant Disorders, National Center on Birth Defects and Developmental Disabilities, CDC, Atlanta, Georgia, USA
| |
Collapse
|