The rise of multidrug-resistant (MDR) pathogens in animal diseases poses a severe threat to veterinary care and public health, necessitating the development of alternative therapeutic strategies. Traditional antimicrobial treatments are becoming increasingly less effective, creating an urgent need for innovative solutions. One among several other promising avenues is the use of plant-based nanoparticles (NPs), which exhibit powerful antimicrobial properties while offering a sustainable and low-toxicity approach. These nanoparticles, synthesized via green methods using plant-derived phytochemicals as natural reducing and stabilizing agents, provide an eco-friendly, cost-effective, and biocompatible option for addressing MDR pathogens. Additionally, the physicochemical properties of these nanoparticles, including size, shape, and surface characteristics, can be fine-tuned to enhance their antimicrobial potency and target-specific action. This review explores the potential of plant-based nanoparticles as a groundbreaking strategy for tackling MDR pathogens in animal diseases, focusing on their mechanisms of action, green synthesis techniques, and applications in veterinary medicine. By optimizing synthesis processes, assessing toxicity, and evaluating in vivo efficacy, plant-based nanoparticles could emerge as an essential tool in the fight against antimicrobial resistance (AMR) in animals, with implications for global health.

Antimicrobial resistance is one of the most serious contemporary global health concerns, threatening the effectiveness of existing antibiotics and resulting in morbidity, mortality, and economic burdens. This review examines the contribution of nanomaterial-based drug delivery systems to solving the problems associated with bacterial resistance and provides a thorough overview of their mechanisms of action, efficiency, and perspectives for the future. Owing to their unique physicochemical properties, nanomaterials reveal new ways of passing through the traditional mechanisms of bacterial defence connected to the permeability barrier of membranes, efflux pumps, and biofilm formation. This review addresses the different types of nanomaterials, including metallic nanoparticles, liposomes, and polymeric nanoparticles, in terms of their antimicrobial properties and modes of action. More emphasis has been placed on the critical discussion of recent studies on such active systems. Both in vitro and in vivo models are discussed, with particular attention paid to multidrug-resistant bacteria. This review begins by reviewing the urgency for antimicrobial resistance (AMR) by citing recent statistics, which indicate that the number of deaths and reasons for financial losses continue to increase. A background is then provided on the limitations of existing antibiotic therapies and the pressing need to develop innovative approaches. Nanomaterial-based drug delivery systems have been proposed as promising solutions because of their potential to improve drug solubility, stability, and targeted delivery, although side effects can also be mitigated. In addition to established knowledge, this review also covers ongoing debates on the continuous risks associated with the use of nanomaterials, such as toxicity and environmental impact. This discussion emphasizes the optimization of nanomaterial design to target specific bacteria, and rigorous clinical trials to establish safety and efficacy in humans. It concludes with reflections on the future directions of nanomaterial-based drug delivery systems in fighting AMR, underlining the need for an interdisciplinary approach, along with continuous research efforts to translate these promising technologies into clinical practice. As the fight against bacterial resistance reaches its peak, nanomaterials may be the key to developing next-generation antimicrobial therapies.

Antimicrobial resistance (AMR) is a critical global health challenge, particularly in Sudan, where the overuse and misuse of antibiotics have driven the rise of multidrug-resistant (MDR) pathogens. Conventional antimicrobial strategies often fall short due to rapid resistance development and limited efficacy, highlighting the need for novel approaches. Nanotechnology offers promising alternatives, with silver nanoparticles (AgNPs) demonstrating potent broad-spectrum antimicrobial activity. This study aims to develop an eco-friendly synthesis of AgNPs using Candida parapsilosis (C. parapsilosis), an untapped yeast strain isolated from Sudanese soil, to combat AMR.

Biosynthesis of AgNPs using C. parapsilosis was successfully confirmed through UV-Vis spectroscopy, X-ray diffraction (XRD), and high-resolution transmission electron microscopy (HRTEM), revealing well-defined nanoparticles. The biosynthesized AgNPs exhibited strong antibacterial activity against both ATCC reference strains and MDR clinical isolates of Gram-positive and Gram-negative bacteria, with inhibition zones increasing in a concentration-dependent manner. At optimal concentrations, inhibition zones reached 29 mm for Pseudomonas aeruginosa (P.aeruginosa) (ATCC 27853), while clinical isolates of Salmonella typhi (S. typhi) (24.5 ± 0.58 mm) and Escherichia coli (E. coli) (23.8 ± 0.79 mm) exhibited significant susceptibility. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays demonstrated potent bactericidal activity, particularly against E. coli and Klebsiella pneumoniae (K. pneumoniae) at 0.3125 mg/mL. Furthermore, AgNPs synergistically enhanced the efficacy of conventional antibiotics in a species- and antibiotic-dependent manner. The strongest synergy was observed in Enterococcus faecalis (E. faecalis) (up to 9.84-fold with Colistin) and Acinetobacter baumannii (A. baumannii) (up to 5.11-fold with Ceftazidime), suggesting that AgNP-enhanced antibiotic efficacy varies depending on bacterial species, nanoparticle synthesis method, and antibiotic type.

This study presents a novel and sustainable approach to tackling AMR by leveraging Sudanese yeast strains for the green synthesis of AgNPs. The findings underscore the potential of AgNPs as an effective antibacterial agent, both independently and in combination with conventional antibiotics, to combat MDR pathogens. By integrating microbiology and nanotechnology, this research offers a cost-effective and environmentally friendly solution for AMR mitigation. These findings provide a strong foundation for future clinical applications and public health interventions, particularly in resource-limited settings.

The emergence of multi-drug-resistant microorganisms presents a serious threat to infection control, for which new antimicrobial strategies are urgently needed. Herein, the antimicrobial activities of copper oxide nanoparticles capped with curcumin (Cur-CuO NPs) and copper oxide nanoparticles capped with chitosan (CS-CuO NPs) were investigated. They were prepared via the co-precipitation method. A total of 180 clinical ICU patients were found to have 70% Gram-negative and 30% Gram-positive isolates. Antimicrobial susceptibility testing indicated resistance of these isolates to 14 among the 21 tested antibiotics. Physicochemical properties of the curcumin-capped (Cur-CuO NPs) and chitosan-capped (CS-CuO NPs) copper oxide nanoparticles were identified using UV-vis spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta-potential (ζ), and Fourier transform infrared (FT-IR) spectroscopy. Cur-CuO- and CS-CuO-NPs exhibited potent antimicrobial efficacy, wherein CS-CuO NPs were found to possess a lower minimum inhibitory concentration (MIC) (3.9-15.6 μg mL-1) than Cur-CuO NPs (14.5-31.2 μg mL-1). Biocompatibility assay showed that Cur-CuO NPs were safer with an IC50 dose of 74.17 μg mL-1 than CS-CuO NPs with an IC50 dose of 41.01 μg mL-1. Results revealed that the Cur-CuO- and CS-CuO-NPs have the potential to be safely used as effective antimicrobial agents in clinical applications at low concentrations (6.25-12.5 μg mL-1).

Methicillin-resistant Staphylococcus aureus (MRSA) is challenging modern antimicrobial therapy due to its high antimicrobial resistance. Nutraceuticals have gained a lot of interest and their incorporation into nanoparticles further improves their efficacy. This study aimed to evaluate the antibacterial activity of linalool-based lipid nanocapsules loaded with resveratrol (LIN-LNC-RES) as a synergistic strategy against MRSA. LIN-LNC-RES were prepared by the phase inversion temperature method and characterized for their colloidal properties, in vitro release, and stability. The antibacterial and antibiofilm activity against S. aureus and different MRSA clinical isolates were investigated. Furthermore, scanning electron microscopy (SEM) imaging for visualization of biofilm formation and bacterial membrane integrity as well as mechanistic investigation using quantitative real-time polymerase chain reaction (qRT-PCR) analysis were performed. LIN-LNCs-RES demonstrated favorable properties with a size of 35.19 ± 0.72 nm, PDI of 0.09 ± 0.02 and a zeta potential of -2.53 ± 0.07 mV with RES 98% EE. They showed a controlled release of RES over 24 h and were stable at 4 °C for 3 months. Compared to free drug, LIN-LNC-RES showed a 4-fold decrease in MIC values and 10-fold decrease in half maximal biofilm inhibitory concentration value. Biofilm eradication assay showed superiority of LIN-LNC-RES over RES against all isolates with disrupted bacterial membranes as revealed by SEM. Mechanistically, qRT-PCR showed that LIN-LNC-RES significantly reduced RNAIII gene expression as well as the expression of SaeRS two component system, potentially affecting quorum sensing and virulence factors expression. RES-loaded LIN-based nanosystem offers a great potential for combating MRSA infections, neutralizing its virulence activity hence, overcoming antimicrobial resistance.

Wound healing is a sophisticated and dynamic process carried out by a myriad of cellular activities that work together in a coordinated manner to effectively repair damaged tissue. It involves a cascade process involving hemostasis, inflammation, granulation, maturation, and remodeling. However, in the case of chronic wounds, owing to the delayed wound healing process, various microbes invade the wound area and produce biofilms that hinder the healing process. Owing to rapid advancements in nanotechnology, several nanomaterials with diverse formulations have been investigated for wound healing. Among them, silver nanoparticles (AgNPs) have shown excellent properties, as they have unique physiochemical properties that address the problems associated with wound healing. The antibacterial and antioxidant properties of silver greatly enhance wound-care diagnostics. The use of medicinal plants for green synthesis of AgNPs has been widely researched, with these plants serving as both reducing and stabilizing agents in the nanoparticle formation process. This review focuses on different wound types, problems related to wounds, green-synthesized AgNPs using medicinal plants, and their limitations and advantages in wound dressing formulations. This study aims to provide the scientific community with a directional view in analyzing the role and importance of green-synthesized AgNPs in wound care.

Antibiotic-resistant Pseudomonas aeruginosa is a pathogen notorious for its resilience in clinical settings due to biofilm formation, efflux pumps, and the rapid acquisition of resistance genes. With traditional antibiotic therapy rendered ineffective against Pseudomonas aeruginosa infections, we explore alternative therapies that have shown promise, including antimicrobial peptides, nanoparticles and quorum sensing inhibitors. While these approaches offer potential, they each face challenges, such as specificity, stability, and delivery, which require careful consideration and further study. We also delve into emerging alternative strategies, such as bacteriophage therapy and CRISPR-Cas gene editing that could enhance targeted treatment for personalized medicine. As most of them are currently in experimental stages, we highlight the need for clinical trials and additional research to confirm their feasibility. Hence, we offer insights into new therapeutic avenues that could help address the pressing issue of antibiotic-resistant Pseudomonas aeruginosa, with an eye toward practical applications in future healthcare.

Antimicrobial resistance (AMR) is a critical public health concern that arises when microorganisms evolve mechanisms to evade the effects of antibiotics, thereby rendering conventional treatments ineffective. This growing challenge underscores the urgent need for novel therapeutic approaches. Nanotechnology, particularly when combined with environmentally sustainable practices such as green synthesis, reduces the use of toxic substances and minimises waste, offering a promising solution. This review explores the green synthesis of antimicrobial nanoparticles using flavonoids-natural compounds with substantial biological activity-as reducing and stabilising agents. By systematically analysing articles from PubMed, Scopus, Web of Science, and Embase, 10 key studies were identified. The primary nanoparticles examined were metallic, including silver, gold, copper, and metallic, which demonstrated notable efficacy against pathogens such as S. aureus, E. coli, and P. aeruginosa. The results support that green-synthesised nanoparticles represent a viable strategy to combat AMR, offering an effective and eco-friendly alternative for developing antimicrobial agents.

Respiratory diseases remain challenging to treat, with current efforts primarily focused on managing symptoms rather than maintaining overall lung health. Traditional treatment methods, such as oral or parenteral administration of antiviral, antibacterial, and anti-inflammatory drugs, face limitations. These include difficulty in delivering therapeutic agents to pathogens residing deep in the airways and the risk of severe side effects due to high systemic drug concentrations. The growing threat of drug-resistant pathogens further complicates infection management.

The lung's large surface area offers an attractive target for inhalation-based drug delivery. Nanoparticles (NP) enable uniform and sustained drug distribution across the alveolar network, overcoming challenges posed by complex lung anatomy. Recent breakthroughs in nanorobots (NR) have demonstrated precise navigation through biological environments, delivering therapies directly to affected lung areas with enhanced accuracy. Nanotechnology has also shown promise in treating lung cancer, with nanoparticles engineered to overcome biological barriers, improve drug solubility, and enable controlled drug release.

This review explores the progress of NP and NR in addressing challenges in pulmonary drug delivery. These innovations allow targeted delivery of nucleic acids, drugs, or peptides to the pulmonary epithelium with unprecedented accuracy, offering significant potential for improving therapeutic effectiveness in respiratory disorders.

In situ-gelling antibacterial hydrogels are reported in which two antibacterial entities (quaternary ammonium (QA) groups and the antibiotic ciprofloxacin (CIP)) are tethered to a single precursor based on the anti-fouling polymer poly(oligoethylene glycol methacrylate) (POEGMA). Synergism between the QA and CIP tethers is demonstrated to enable broad-spectrum killing and/or disinfection of both gram-positive and gram-negative bacteria both in vitro and in vivo while also supporting improved functional recovery of uninjured skin morphology. Coupled with the suitable mechanics, swelling capacity, and stability of the gels, the multi-mechanism antibacterial properties of the hydrogels offer promise for treating or preventing infections of burn wounds.

This study aimed to synthesize silver nanoparticles (AgNPs) using the postbiotic of the Ligilactobacillus salivarius KC27L strain and evaluate their multifunctional biological properties. The use of L. salivarius, a probiotic bacterium known for its ability to produce a wide range of metabolites, plays a crucial role in this process by acting as a natural, eco-friendly reducing, and stabilizing agent during AgNP synthesis. This approach not only eliminates the need for hazardous chemicals typically used in nanoparticle synthesis but also enhances the biocompatibility and biological efficacy of the resulting nanoparticles. Synthesized AgNPs were analyzed by Fourier transform infrared spectroscopy, FTIR (metabolites of postbiotic); UV-vis (peak of 435 nm); scanning electron microscope, SEM; transmission electron microscopy, TEM (spherical shapes, sizes < 50 nm), energy-dispersive spectrometry, EDS (peak at 3 keV); and zeta potential (- 18.6 mV). These nanoparticles (0.156-40 mg/mL) were evaluated for the antimicrobial and anti-biofilm activities against Escherichia coli ATCC 11229, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 35984, and Streptococcus mutans ATCC 25175, and antioxidant activities using four different methods (2,2-diphenyl-1-picrylhydrazyl free radical scavenging, metal ion chelating, hydroxyl radical scavenging, and superoxide anion scavenging activities). Also, the cytotoxic activity was investigated against a normal cell line (L929) for 24, 48, and 72 h. At a concentration of 40 mg/mL, the AgNPs demonstrated the highest antimicrobial efficacy, with inhibition zones measured as 14.9 mm for P. aeruginosa, 9.5 mm for E. coli, 15.7 mm for S. epidermidis, and 12.9 mm for S. mutans. The AgNPs exhibited anti-biofilm activities against all Gram-positive and Gram-negative bacteria strains studied. According to the DPPH method, the highest antioxidant activity was determined at 40 mg/mL AgNP concentration (80.93%). AgNPs were found to have no toxic effect at low concentrations (0.39-25 µg/mL). Biogenic synthesized AgNPs could be used in biotechnological applications (biomaterials, health, environmental, etc.) with antibacterial, anti-biofilm, antioxidant, and nontoxic properties. However, further research is needed to understand the mechanisms of action of the particles fully.

Zinc has attracted significant attention as a versatile material with potential applications in various fields, particularly in biomedical engineering. Despite its desirable characteristics, such as biodegradability and biocompatibility, the inherently low mechanical strength of zinc has been a major limitation for its broader use in clinical applications. To address this issue and enhance its mechanical performance without compromising its biocompatibility, a novel composite material was developed by mixing zinc oxide (ZnO) with zinc (Zn). ZnO is widely recognized for its high chemical stability, non-toxicity, and antimicrobial properties, making it an excellent additive for biomedical materials. In this study, Zn-ZnO nanocomposites were fabricated by uniformly dispersing ZnO nanoparticles into molten zinc using an ultrasonic processor. The uniform distribution of ZnO nanoparticles within the zinc matrix was confirmed, and the resulting nanocomposites demonstrated remarkable improvements in mechanical properties. Specifically, the hardness and tensile strength of the Zn-ZnO nanocomposites were increased by approximately 90% and 160%, respectively, compared to pure zinc. To evaluate the biodegradation behavior of the materials, both pure zinc and Zn-ZnO nanocomposite samples were immersed in phosphate-buffered saline (PBS) at 37 °C, simulating physiological conditions. The degradation rate was assessed by measuring the weight loss of the material over time. The biodegradation rate of the Zn-ZnO nanocomposites was found to be nearly identical to that of pure zinc under identical conditions, indicating that the addition of ZnO did not adversely affect the degradability of the material. These findings suggest that Zn-ZnO nanocomposites offer a promising solution for biomedical applications by combining improved mechanical properties with maintained biodegradability and biocompatibility.

In recent years, antimicrobial resistance (AMR) has become an alarming threat to global health as notable increase in morbidity and mortality has been ascribed to the emergence of superbugs. The increase in microbial resistance because of harboured or inherited resistomes has been complicated by the lack of new and effective antimicrobial agents, as well as misuse and failure of existing ones. These problems have generated severe and growing public health concern, due to high burden of bacterial infections resulting from scarce financial resources and poor functioning health systems, among others. It is therefore, highly pressing to search for novel and more efficacious alternatives for combating the action of these super bacteria and their infection. The application of metallic nanoparticles (MNPs) with their distinctive physical and chemical attributes appears as promising tools in fighting off these deadly superbugs. The simple, inexpensive and eco-friendly model for enhanced biologically inspired MNPs with exceptional antimicrobial effect and diverse mechanisms of action againsts multiple cell components seems to offer the most promising option and said to have enticed many researchers who now show tremendous interest. This synopsis offers critical discussion on application of MNPs as the foremost intervening strategy to curb the menace posed by the spread of superbugs. As such, this review explores how antimicrobial properties of the metallic nanoparticles which demonstrated considerable efficacy against several multi-drugs resistant bacteria, could be adopted as promising approach in subduing the threat of AMR and harvoc resulting from the spread of superbugs.

The escalating threat of antimicrobial resistance (AMR), particularly among foodborne pathogens such as Escherichia coli, Salmonella enterica, and Listeria monocytogenes, necessitates innovative solutions beyond conventional antimicrobials. Silver nanoparticles (AgNPs) have garnered significant attention for their broad-spectrum antimicrobial efficacy, ability to target multidrug-resistant strains, and versatile applications across the food sector. This review critically examines AgNPs' integration into food safety strategies, including their roles in antimicrobial food packaging, agricultural productivity enhancement, and livestock disease mitigation. Key advancements in eco-friendly synthesis methods, leveraging algae, agricultural byproducts, and microbial systems, are highlighted as pathways to address scalability, sustainability, and cost constraints. However, the potential risks of silver bioaccumulation, environmental toxicity, and regulatory challenges present significant barriers to their widespread implementation. By reviewing cutting-edge research, this review provides a comprehensive analysis of AgNP efficacy, safety, and commercial viability, proposing a roadmap for overcoming current limitations. It calls for collaborative, interdisciplinary efforts to bridge technological, ecological, and regulatory gaps, positioning AgNPs as a transformative solution for combating AMR and ensuring global food security.

Liposomal hydrogels are novel drug delivery systems that comprise preformed liposomes incorporated in hydrogels destined for mostly localized drug therapy, herewith antimicrobial therapy. The formulation benefits from versatility of liposomes as lipid-based nanocarriers that enable delivery of various antimicrobials of different lipophilicities, and secondary vehicle, hydrogel, that assures better retention time of formulation at the infection site. Especially in an era of alarming antimicrobial resistance, efficient localized antimicrobial therapy that avoids systemic exposure of antimicrobial and related side effects is crucial.

We provide an overview of liposomal hydrogels that were developed for superior delivery of antimicrobials at different infections sites, with focus on skin and vaginal infections. The review summarizes the challenges of infection site and most common infection-causing pathogens and offers commentary on most relevant features the formulation needs to optimize to increase the therapy outcome. We discuss the impact of liposomal composition, size, and choice of polymer-forming hydrogel on antimicrobial outcome based on the literature overview and own experience in the field.

Liposomal hydrogels offer improved therapy outcome in localized antimicrobial therapy. By fine-tuning of liposomal as well as hydrogel properties, formulations with superior performance can be optimized targeting specific infection site.

Polymeric nanoparticles (NPs) are traditionally formulated using batch methodologies that are poorly scalable and require time consuming, hands-on purification procedures. Here, we prepared poly(lactic acid) (PLA)-based polymeric NPs using a scalable microfluidics-based method and systematically investigated the impact of purification method (centrifugation versus tangential flow filtration (TFF)) to remove poly(vinyl alcohol) (PVA) on macrophage uptake, anti-inflammatory effects, biodistribution, and protein corona formation. TFF purification demonstrated significantly higher recovery of NPs compared to the centrifugation method, with little-to-no aggregation observed. PVA removal efficiency was superior with centrifugation, although TFF was comparable. NP cellular association, in vitro anti-inflammatory activity, and in vivo biodistribution studies suggested purification method-dependent alterations, which were correlated with protein corona profiles. This study underscores the potential of TFF, combined with microfluidics, as an efficient and high-yield purification method for NPs, and reveals the need for extensive confirmation of NP biological activity alongside physicochemical properties when developing NP therapeutics at-scale.

A significant global health crisis is predicted to emerge due to antimicrobial resistance by 2050, with an estimated 10 million deaths annually. Increasing antibiotic resistance necessitates continuous therapeutic innovation as conventional antibiotic treatments become increasingly ineffective. The naturally occurring antibacterial, antifungal, and antiviral compounds offer a viable alternative to synthetic antibiotics. This review presents bacterial resistance mechanisms, nanocarriers for drug delivery, and plant-based compounds for nanoformulations, particularly nanoantibiotics (nAbts). Green synthesis of nanoparticles has emerged as a revolutionary approach, as it enhances the effectiveness, specificity, and transport of encapsulated antimicrobials. In addition to minimizing systemic side effects, these nanocarriers can maximize therapeutic impact by delivering the antimicrobials directly to the infection site. Furthermore, combining two or more antibiotics within these nanoparticles often exhibits synergistic effects, enhancing the effectiveness against drug-resistant bacteria. Antimicrobial agents are routinely obtained from secondary metabolites of plants, including essential oils, phenols, polyphenols, alkaloids, and others. Integrating plant-based antibacterial agents and conventional antibiotics, assisted by suitable nanocarriers for codelivery, is a potential solution for addressing bacterial resistance. In addition to increasing their effectiveness and boosting the immune system, this synergistic approach provides a safer and more effective method of tackling future bacterial infections.

Antimicrobial resistance is a critical global challenge in the 21st century, validating Sir Alexander Fleming's warning about the misuse of antibiotics leading to resistant microbes. With a dwindling arsenal of effective antibiotics, it is imperative to concentrate on alternative antimicrobial strategies. Previous studies have not comprehensively discussed the advantages and limitations of various strategies, including bacteriophage therapy, probiotics, immunotherapies, photodynamic therapy, essential oils, nanoparticles and antimicrobial peptides (AMPs) within a single review. This review addresses that gap by providing an overview of these various non-antibiotic antimicrobial strategies, highlighting their pros and cons, with a particular emphasis on antimicrobial peptides (AMPs). We explore the mechanism of action of AMPs against bacteria, viruses, fungi and parasites. While these peptides hold significant promise, their application in mainstream drug development is hindered by challenges such as low bioavailability and potential toxicity. However, advancements in peptide engineering and chemical modifications offer solutions to enhance their clinical utility. Additionally, this review presents updates on strategies aimed at improving the cost, stability and selective toxicity of AMPs through the development of peptidomimetics. These molecules have demonstrated effective activity against a broad range of pathogens, making them valuable candidates for integration into surface coatings to prevent device-associated infections. Furthermore, we discuss various approaches for attaching and functionalising these peptides on surfaces. Finally, we recommend comprehensive in vivo studies to evaluate the efficacy of AMPs and their mimetics, investigate their synergistic combinations with other molecules and assess their potential as coatings for medical devices.

Introduction. Fungal infections caused by yeast have increased in recent decades, becoming a major threat to public health.Hypothesis/Gap Statement. Antifungal therapy represents a challenging problem because, in addition to presenting many side effects, fungal resistance has been increasing in recent years. As a result, the search for new therapeutic agents has advanced with the use of new technologies such as nanoparticles (NPs).Aim. Synthesize, characterize and evaluate the antifungal potential of naringenin (NAR)-stabilized silver NPs.Methodology. The biosynthesis of NPs was stabilized using the NAR molecule and an aqueous solution of silver nitrate. The characterization of silver nanoparticles (AgNPs) was performed using different methods, which include UV-visible spectroscopy, powder X-ray diffraction (XRD), transmission electron microscopy, zeta potential measurements and Fourier transform infrared (FTIR) spectroscopy. Antifungal activity was evaluated against clinical isolates of Candida albicans by determining the MIC and the minimum fungicidal concentration (MFC).Results. The AgNP NAR showed a colloidal appearance with an average size of 14.71 nm and zeta potential measured at -33.3 mV, indicating a highly stable suspension. XRD analysis confirmed the crystal structure. FTIR spectra showed the presence of several functional groups of plant compounds, which play an important role in the coating and bioreduction processes. The antifungal activity against C. albicans showed an MIC of 3.55 µg ml-1 and an MFC of 7.1 µg ml-1. According to the growth kinetic assay in 12 h, there was a reduction of ~50% (<3 log10). Furthermore, AgNP NAR did not show mutagenic potential.Conclusion. The AgNP NAR obtained presented ideal characteristics for biomedical applications, good stability and promising antimicrobial activity.

Last decade has witnessed impressive progress in the fields of medicine and bioengineering with the aid of nanomaterials. Nanomaterials are favoured for their improved bio-chemical as well as mechanical properties with tremendous applications in biomedical domains such as disease diagnosis, targeted drug delivery, medical imaging, in vitro diagnostics, designing innovatory cross-functional implants and regenerative tissue engineering. The current situation insists upon crafting nanotools that are capable of catering to biological needs and construct more efficient biomedical strategies. In the recent years, surface functionalization and capping with biomolecules has initiated substantial interest towards research. In this regard, search of suitable biofunctionalized nanoparticles seem to be like finding pearls from ocean. Conjugating biological molecules with inorganic materials has paved the way for unravelling innovative functional materials with dramatically improved properties and a wide range of uses. Inorganic nanoparticles such as metals, metal oxides, as well as quantum dots have been hybridised or conjugated with biomolecules such as proteins, peptides, carbohydrates, and nucleic acids. The present review reports on various biomolecule functionalized inorganic nanomaterials highlighting the biomolecule-inorganic nanoparticle interaction studies, the mechanism of functionalization, antimicrobial efficacy of the functionalised nanoconjugates and its use in various biomedical applications.

The growth of nanoscale sciences enables us to define and design new methods and materials for a better life. Health and disease prevention are the main issues in the human lifespan. Some nanoparticles (NPs) have antimicrobial properties that make them useful in many applications. In recent years, NPs have been used as antibiotics to overcome drug resistance or as drug carriers with antimicrobial features. They can also serve as antimicrobial coatings for implants in different body areas. The antimicrobial feature of NPs is based on different mechanisms. For example, the oxidative functions of NPs can inhibit nucleic acid replication and destroy the microbial cell membrane as well as interfere with their cellular functions and biochemical cycles. On the other hand, NPs can disrupt the pathogens' lifecycle by interrupting vital points of their life, such as virus uncoating and entry into human cells. Many types of NPs have been tested by different scientists for these purposes. Silver, gold, copper, and titanium have shown the most ability to inhibit and remove pathogens inside and outside the body. In this review, the authors endeavor to comprehensively describe the antimicrobial features of NPs and their applications for different biomedical goals.

The extensive use of antibiotics during recent years has led to antimicrobial resistance development, a significant threat to global public health. It is estimated that around 1.27 million people died worldwide in 2019 due to infectious diseases caused by antibiotic-resistant microorganisms, according to the WHO. It is estimated that 700,000 people die each year worldwide, which is expected to rise to 10 million by 2050. Therefore, new and efficient antimicrobials against resistant pathogenic bacteria are urgently needed. Antimicrobial peptides (AMPs) present a broad spectrum of antibacterial effects and are considered potential tools for developing novel therapies to combat resistant infections. However, their clinical application is currently limited due to instability, low selectivity, toxicity, and limited bioavailability, resulting in a narrow therapeutic window. Here we describe an overview of the clinical application of AMPs against resistant bacterial infections through nanoformulation. It evaluates metal, polymeric, and lipid AMP delivery systems as promising for the treatment of resistant bacterial infections, offering a potential solution to the aforementioned limitations.

Synthesis of metal oxide nanoparticles-polymer nanocomposites is an emerging strategy in nanotechnology to improve targeted delivery and reduce the toxicity of nanoparticles. In this study, we report biological effects of previously described hybrid nanocomposites containing dextran-graft-polyacrylamide/zinc oxide nanoparticles (D-PAA/ZnO NPs) prepared from zinc sulfate (D-PAA/ZnONPs(SO42-)) and zinc acetate (D-PAA/ZnONPs(-OAc)) focusing primarily on their antimicrobial activity. D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems were tested in a complex way to assess their antioxidant activity (DPPH assay), antidiabetic potential (α-amylase inhibition), DNA cleavage activity, antimicrobial, and antibiofilm activity. In addition, the toxicity of D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems against primary murine splenocytes was tested using MTT assay. The studied nanosystems inhibited E.coli growth. For all the investigated strains, minimum inhibitory concentrations (MICs) of D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) were in the range of 8 mg/L-128 mg/L and 16 mg/L-128 mg/L, respectively. The nanocomposites demonstrated effective antibiofilm properties as 94.27% and 86.43%. The compounds showed good antioxidant, anti-α-amylase, and DNA cleavage activities. D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems reduced cell viability and promoted cell death of primary murine spleen cells at concentrations higher than those that proved to be antibacterial indicating the presence of therapeutic window. D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems show antioxidant, antidiabetic, DNA cleavage, antimicrobial, and antibiofilm activity against the background of good biocompatibility suggesting the presence of therapeutic potential, which should be further investigated in vivo.

One of the most significant medical advancements of the 20th century was the discovery of antibiotics, which continue to play a vital tool in the treatment and prevention of diseases in humans and animals. However, the imprudent use of antibiotics in all fields of One-Health and concerns about antibiotic resistance among bacterial pathogens have raised interest in antibiotic use restrictions on a global scale. Despite the failure of conventional antimicrobial agents, only about 15 new antibiotics have been introduced clinically since year 2000 to date. Moreover, there has been reports of resistance to some of these new antibiotics. This has necessitated a need to search for alternative strategies to combat antimicrobial resistant pathogens. Thus, this review compiles and evaluates the approaches-natural compounds, phage treatment, and nanomaterials-that are being used and/or suggested as the potential substitutes for conventional antibiotics.

Similar to mammalian cells, most bacteria can release nano-sized membrane vesicles (MVs) into the extracellular environment. MVs contain lipids, bioactive proteins, nucleic acids, and metabolites, and play important roles in microbial physiology. MVs have great potential for immunotherapeutic applications, such as bacterial vaccines and cancer immunotherapy. However, because of the diversity in content and heterogeneity in size of MVs, the clinical application of MVs has been limited. Recently, the use of MVs combined with nanoparticles (NPs) has been shown to be effective in improving the homogeneity, stability and function of MVs. In this review, we focus on studies of MVs combined with NPs (MV-NPs) and describe the use of these MV-NPs in biotechnology, especially in bacterial vaccine and cancer immunotherapy.

The rise of the populations of antibiotic resistant bacteria represents an increasing threat to human health. In addition to the synthesis of new antibiotics, which is an extremely expensive and time-consuming process, one of the ways to combat bacterial infections is the use of gold nanoparticles (Au NPs) as the vehicles for targeted delivery of therapeutic drugs. Since such a strategy requires the investigation of the effect of Au NPs (with and without drugs) on both bacterial and human cells, we investigated how the presence of coating-free Au NPs affects the physicochemical properties of lipid membranes that model prokaryotic (PRO) and eukaryotic (EU) cells. PRO/EU systems prepared as multilamellar liposomes (MLVs) and hybrid structures (HSs) from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylglycerol (DPPG)/1,2-dipalmitoyl-sn-glycero-3-phosphoserine (DPPS) in the absence (MLVs)/presence (HSs) of differently distributed Au NPs (sizes ∼20 nm) reported stabilization of the gel phase of PRO systems in comparison with EU one (DSC data of PRO/EU were Tm(MLVs) ≈ 41.8 °C/42.0 °C, Tm¯ (HSs) ≈ 43.1 °C/42.4 °C, whereas UV-Vis response Tm(MLVs) ≈ 41.5 °C/42.0 °C, Tm¯ (HSs) ≈ 42.9 °C/41.1 °C). Vibrational spectroscopic data unraveled a substantial impact of Au NPs on the non-polar part of lipid bilayers, emphasizing the increase of kink and gauche conformers of the hydrocarbon chain. By interpreting the latter as Au NPs-induced defects, which exert the greatest effect when Au NPs are found exclusively outside the lipid membrane, these findings suggested that Au NPs reduced the compactness of EU-based lipid bilayers much more than in analogous PRO systems. Since the uncoated Au NPs manifested adverse effects when applied as antimicrobials, the results obtained in this work contribute towards recognizing AuNP functionalization as a strategy in tuning and reversing this effect.

Biogenic metallic nanoparticles (NPs) have garnered significant attention in recent years due to their unique properties and various applications in different fields. NPs, including gold, silver, zinc oxide, copper, titanium, and magnesium oxide NPs, have attracted considerable interest. Green synthesis approaches, utilizing natural products, offer advantages such as sustainability and environmental friendliness. The theranostics applications of these NPs hold immense significance in the fields of medicine and diagnostics. The review explores intricate cellular uptake pathways, internalization dynamics, reactive oxygen species generation, and ensuing inflammatory responses, shedding light on the intricate mechanisms governing their behaviour at a molecular level. Intriguingly, biogenic metallic NPs exhibit a wide array of applications in medicine, including but not limited to anti-inflammatory, anticancer, anti-diabetic, anti-plasmodial, antiviral properties and radical scavenging efficacy. Their potential in personalized medicine stands out, with a focus on tailoring treatments to individual patients based on these NPs' unique attributes and targeted delivery capabilities. The article culminates in emphasizing the role of biogenic metallic NPs in shaping the landscape of personalized medicine. Harnessing their unique properties for tailored therapeutics, diagnostics and targeted interventions, these NPs pave the way for a paradigm shift in healthcare, promising enhanced efficacy and reduced adverse effects.

Bacterial resistance to antibiotics is a significant challenge that is associated with increased morbidity and mortality. Gram-negative bacteria are particularly problematic due to an outer membrane (OM). Current alternatives to antibiotics include antimicrobial peptides or proteins and multifunctional systems such as dendrimers. Antimicrobial proteins such as lysins can degrade the bacterial cell wall, whereas dendrimers can permeabilize the OM, enhancing the activity of endolysins against gram-negative bacteria. In this study, we present a three-stage action of endolysin combined with two different carbosilane (CBS) silver metallodendrimers, in which the periphery is modified with N-heterocyclic carbene (NHC) ligands coordinating a silver atom. The different NHC ligands contained hydrophobic methyl or N-donor pyridyl moieties. The effects of these endolysin/dendrimer combinations are based on OM permeabilization, peptidoglycan degradation, and reactive oxygen species production. The results showed that CBS possess a permeabilization effect (first action), significantly reduced bacterial growth at higher concentrations alone and in the presence of endolysin, increased ROS production (second action), and led to bacterial cell damage (third action). The complex formed between the CHAP domain of endolysin and a CBS silver metallodendrimer, with a triple mechanism of action, may represent an excellent alternative to other antimicrobials with only one resistance mechanism.

With the emergence of drug-resistance, there is a need for novel anti-bacterials or to enhance the efficacy of existing drugs. In this study, Patuletin (PA), a flavanoid was loaded onto Gallic acid modified Zinc oxide nanoparticles (PA-GA-ZnO), and evaluated for antibacterial properties against Gram-positive (Bacillus cereus and Streptococcus pneumoniae) and Gram-negative (Samonella enterica and Escherichia coli) bacteria. Characterization of PA, GA-ZnO and PA-GA-ZnO' nanoparticles was accomplished utilizing fourier-transform infrared spectroscopy, efficiency of drug entrapment, polydispersity index, zeta potential, size, and surface morphology analysis through atomic force microscopy. Using bactericidal assays, the results revealed that ZnO conjugation displayed remarkable effects and enhanced Patuletin's effects against both Gram-positive and Gram-negative bacteria, with the minimum inhibitory concentration observed at micromolar concentrations. Cytopathogenicity assays exhibited that the drug-nanoconjugates reduced bacterial-mediated human cell death with minimal side effects to human cells. When tested alone, drug-nanoconjugates tested in this study showed limited toxic effects against human cells in vitro. These are promising findings, but future work is needed to understand the molecular mechanisms of effects of drug-nanoconjugates against bacterial pathogens, in addition to in vivo testing to determine their translational value. This study suggests that Patuletin-loaded nano-formulation (PA-GA-ZnO) may be implicated in a multi-target mechanism that affects both Gram-positive and Gram-negative pathogen cell structures, however this needs to be ascertained in future work.

Bryophyllum pinnatum is used to cure infections worldwide. Although the flavonoids of this plant are well known, it is still unknown how much of the plant's Ag and ZnO nanoparticles are beneficial. In the current research work, silver and zinc oxide nanoparticles were prepared using Bryophyllum pinnatum extract. The synthesized particles were characterized by UV-visible spectroscopy, SEM, EDS, XRD and FTIR. Synthesized particles were subjected to evaluation of their bactericidal and antifungal activity at various doses. Uv vis spectra at 400 nm corresponding to AgNPs confirmed their synthesis. Strong peaks in the EDS spectra of Ag and ZnO indicate the purity of the sample. The scanning electron microscopic images of ZnONPs showed a size of about 60 nm ± 3 nm, which demonstrated the presence of triangular-shaped ZnO nanoparticles. Green synthesized nanoparticles showed bactericidal activity against both Gram-positive (Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Agrobacterium tumifaciens, Salmonella setubal, Enterobacter aerogenes) strains. AgNPs proved to be more effective against Gram-negative bacterial strains compared to Gram-positive owing to MIC values (10 ppm and 20 ppm respectively). Whereas, ZnONPs were found more effective against Gram-positive bacteria with lower MIC values (10 ppm) as compared to Gram-negative ones (20 ppm). Also, the synthesized nanoparticles exhibited moderate dose-dependent antifungal activity against tested fungal strains ranging from 10 to 70%. Cytotoxicity of nanoparticles was found significant using Brine shrimp's lethality assay with IC50 values of 4.09 ppm for AgNPs, 13.72 ppm for ZnONPs, and 24.83 ppm for plant extract. Conclusively, Ag and ZnO nanoparticles were more effective than plant extract and AgNPs had higher activities than those of ZnONPs. Further research is warranted to explore the precise mechanism of action and the potential applications of these nanoparticles in the medical field.

Polymers of natural origin, such as representatives of various polysaccharides (e.g., cellulose, dextran, hyaluronic acid, gellan gum, etc.), and their derivatives, have a long tradition in biomedical applications. Among them, the use of chitosan as a safe, biocompatible, and environmentally friendly heteropolysaccharide has been particularly intensively researched over the last two decades. The potential of using chitosan for medical purposes is reflected in its unique cationic nature, viscosity-increasing and gel-forming ability, non-toxicity in living cells, antimicrobial activity, mucoadhesiveness, biodegradability, as well as the possibility of chemical modification. The intuitive use of clay minerals in the treatment of superficial wounds has been known in traditional medicine for thousands of years. To improve efficacy and overcome the ubiquitous bacterial resistance, the beneficial properties of chitosan have been utilized for the preparation of chitosan-clay mineral bionanocomposites. The focus of this review is on composites containing chitosan with montmorillonite and halloysite as representatives of clay minerals. This review highlights the antibacterial efficacy of chitosan-clay mineral bionanocomposites in drug delivery and in the treatment of topical skin infections and wound healing. Finally, an overview of the preparation, characterization, and possible future perspectives related to the use of these advancing composites for biomedical applications is presented.

Biofilm-producing methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci (MR-CoNS) pose clinical challenges in treating healthcare-associated infections. As alternative antimicrobial options are needed, in this study, we aimed to determine the effect of curcumin-chitosan magnetic nanoparticles (Cur-Chi-MNP) on the biofilms of staphylococcal clinical isolates. MRSA and CoNS clinical isolates were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antimicrobial susceptibility testing was performed using the broth microdilutions. Nanoparticles were synthesized by the co-precipitation of magnetic nanoparticles (MNP) and encapsulated by the ionotropic gelation of curcumin (Cur) and chitosan (Chi). Biofilm inhibition and eradication by nanoparticles, with and without the addition of oxacillin (OXA), were assessed in Staphylococcus strains. Cur-Chi-MNP showed antimicrobial activity against planktonic cells of MRSA and MR-CoNS strains and inhibited MRSA biofilm. The addition of OXA to Cur-Chi-MNP increased the biofilm inhibition and eradication activity against all staphylococcal strains (P = 0.0007), and higher biofilm activity was observed in the early biofilm stages. Cur-Chi-MNP showed antimicrobial and biofilm inhibitory activities against S. aureus. Addition of OXA increased biofilm inhibition and eradication activity against all staphylococcal strains. A combination treatment of Cur-Chi-MNP and OXA could potentially be used to treat staphylococcal biofilm-associated infections in the early stages before the establishment of biofilm bacterial cells.

Aim: We present the synthesis of anti-bacterial gold nanoparticles using chitosan as a dual-functional agent. The resulting ChAuNPs were further modified with a lipopolysaccharide-targeting antibacterial peptide to aid in biocompatibility and specificity.Materials & methods: The nanoparticles' antibacterial activity against Escherichia coli was tested in the presence of a 450 nm laser.Results: Our data suggested that the peptide and laser emissions had a synergistic impact on the gold nanoparticles, resulting in strong antibacterial effects. The study shows that advanced nanomaterials, including chitosan, gold nanoparticles and lipopolysaccharide targeting peptides, can boost antibacterial functions at a low concentration of 250 μg/ml.Conclusion: The findings highlight ChAuNPs' potential as strong antibacterial agents, with targeted alterations critical for maximizing their utilization.

The current burden associated to multidrug resistance, and the emerging superbugs, result in a decreased and even loss of antibiotic efficacy, which poses significant challenges in the treatment of infectious diseases. This situation has created a high demand for the discovery of novel antibiotics that are both effective and safe. However, while antibiotics play a crucial role in preventing and treating diseases, they are also associated with adverse effects. The emergence of multidrug-resistant and the extensive appearance of drug-resistant microorganisms, has become one of the major hurdles in healthcare. Addressing this problem will require the development of at least 20 new antibiotics by 2060. However, the process of designing new antibiotics is time-consuming. To overcome the spread of drug-resistant microbes and infections, constant evaluation of innovative methods and new molecules is essential. Research is actively exploring alternative strategies, such as combination therapies, new drug delivery systems, and the repurposing of existing drugs. In addition, advancements in genomic and proteomic technologies are aiding in the identification of potential new drug targets and the discovery of new antibiotic compounds. In this review, we explore new sources of natural antibiotics from plants, algae other sources, and propose innovative bioinspired delivery systems for their use as an approach to promoting responsible antibiotic use and mitigate the spread of drug-resistant microbes and infections.

Opportunistic pathogenic microbial infections pose a significant danger to human health, which forces people to use riskier, more expensive, and less effective drugs compared to traditional treatments. These may be attributed to several factors, such as overusing antibiotics in medicine and lack of sanitization in hospital settings. In this context, researchers are looking for new options to combat this worrying condition and find a solution. Nanoparticles are currently being utilized in the pharmaceutical sector; however, there is a persistent worry regarding their potential danger to human health due to the usage of toxic chemicals, which makes the utilization of nanoparticles highly hazardous to eukaryotic cells. Multiple nanoparticle-based techniques are now being developed, offering essential understanding regarding the synthesis of components that play a crucial role in producing anti-microbial nanotherapeutic pharmaceuticals. In this regard, green nanoparticles are considered less hazardous than other forms, providing potential options for avoiding the extensive harm to the human microbiome that is prevalent with existing procedures. This review article aims to comprehensively assess the current state of knowledge on green nanoparticles related to antibiotic activity as well as their potential to assist antibiotics in treating opportunistic clinical phytopathogenic illnesses.

The increasing occurrence of infectious diseases caused by antimicrobial resistance organisms urged the necessity to develop more potent, selective, and safe antimicrobial agents. The unique magnetic and tunable properties of iron oxide nanoparticles (IONPs) make them a promising candidate for different theragnostic applications, including antimicrobial agents. Though IONPs act as a nonspecific antimicrobial agent, their antimicrobial activities are directly or indirectly linked with their synthesis methods, synthesizing precursors, size, shapes, concentration, and surface modifications. Alteration of these parameters could accelerate or decelerate the production of reactive oxygen species (ROS). An increase in ROS role production disrupts bacterial cell walls, cell membranes, alters major biomolecules (e.g., lipids, proteins, nucleic acids), and affects metabolic processes (e.g., Krebs cycle, fatty acid synthesis, ATP synthesis, glycolysis, and mitophagy). In this review, we will investigate the antibacterial activity of bare and surface-modified IONPs and the influence of physiochemical parameters on their antibacterial activity. Additionally, we will report the potential mechanism of IONPs' action in driving this antimicrobial activity.

The rise of antibiotic resistance poses a significant threat to public health worldwide, leading researchers to explore novel solutions to combat this growing problem. Nanotechnology, which involves manipulating materials at the nanoscale, has emerged as a promising avenue for developing novel strategies to combat antibiotic resistance. This cutting-edge technology has gained momentum in the medical field by offering a new approach to combating infectious diseases. Nanomaterial-based therapies hold significant potential in treating difficult bacterial infections by circumventing established drug resistance mechanisms. Moreover, their small size and unique physical properties enable them to effectively target biofilms, which are commonly linked to resistance development. By leveraging these advantages, nanomaterials present a viable solution to enhance the effectiveness of existing antibiotics or even create entirely new antibacterial mechanisms. This review article explores the current landscape of antibiotic resistance and underscores the pivotal role that nanotechnology plays in augmenting the efficacy of traditional antibiotics. Furthermore, it addresses the challenges and opportunities within the realm of nanotechnology for combating antibiotic resistance, while also outlining future research directions in this critical area. Overall, this comprehensive review articulates the potential of nanotechnology in addressing the urgent public health concern of antibiotic resistance, highlighting its transformative capabilities in healthcare.

Nanoparticles play a crucial role in the field of nanotechnology, offering different properties due to their surface area attributed to their small size. Among them, silver nanoparticles (AgNPs) have attracted significant attention due to their antimicrobial properties, with applications that date back from ancient medicinal practices to contemporary commercial products containing ions or silver nanoparticles. AgNPs possess broad-spectrum biocidal potential against bacteria, fungi, viruses, and Mycobacterium, in addition to exhibiting synergistic effects when combined with certain antibiotics. The mechanisms underlying its antimicrobial action include the generation of oxygen-reactive species, damage to DNA, rupture of bacterial cell membranes and inhibition of protein synthesis. Recent studies have highlighted the effectiveness of AgNPs against various clinically relevant bacterial strains through their potential to combat antibiotic-resistant pathogens. This review investigates the proteomic mechanisms by which AgNPs exert their antimicrobial effects, with a special focus on their activity against planktonic bacteria and in biofilms. Furthermore, it discusses the biomedical applications of AgNPs and their potential non-preparation of antibiotic formulations, also addressing the issue of resistance to antibiotics.

Metal oxide nanoparticles with photothermal properties have attracted considerable research attention for their use in biomedical applications. Cesium tungsten oxide (Cs0.33WO3) nanoparticles (NPs) exhibit strong absorption in the NIR region due to localized surface plasmon resonance, through which they convert light to heat; hence, they can be applied to photothermal treatment for bacteria and biofilm ablation. Herein, Cs0.33WO3 NPs were synthesized through solid-phase synthesis, and their physical properties were characterized through Zetasizer, energy dispersive X-ray spectroscopy, Fourier transform infrared spectrometer, and scanning and transmission electron microscopy (SEM and TEM, respectively). Burkholderia cenocepacia isolates were cultured in tryptic soy broth supplemented with glucose, and the biofilm inhibition and antibiofilm effects of the NPs were determined using a crystal violet assay and the Cell Counting Kit-8 (CCK-8) assay. The biofilm morphology and viability of NP-treated cultures after NIR irradiation were evaluated through SEM and confocal microscopy, respectively. The cytotoxicity of NPs to human macrophages was also assessed using the CCK-8 assay. The NPs effectively inhibited biofilm formation, with a formation rate of <10% and a viability rate of <50% at the concentration of ≥200 μg/mL. The confocal analysis revealed that NIR irradiation markedly enhanced biofilm cytotoxicity after treatment with the NPs. The assay of cytotoxicity to human macrophages demonstrated the biocompatibility of the NPs and NIR irradiation. In sum, the Cs0.33WO3 NPs displayed effective biofilm inhibition and antibiofilm activity at 200 μg/mL treatment concentration; they exhibited an enhancement effect under the NIR irradiation, suggesting Cs0.33WO3 NPs are a potential candidate agent for NIR-irradiated photothermal treatment in bacterial biofilm inhibition and antibiofilm.

Nanodrug delivery systems (NDDS) continue to be explored as novel strategies enhance therapy outcomes and combat microbial resistance. The need for the formulation of smart drug delivery systems for targeting infection sites calls for the engineering of responsive chemical designs such as dynamic covalent bonds (DCBs). Stimuli response due to DCBs incorporated into nanosystems are emerging as an alternative way to target infection sites, thus enhancing the delivery of antibacterial agents. This leads to the eradication of bacterial infections and the reduction of antimicrobial resistance. Incorporating DCBs on the backbone of the nanoparticles endows the systems with several properties, including self-healing, controlled disassembly, and stimuli responsiveness, which are beneficial in the delivery and release of the antimicrobial at the infection site. This review provides a comprehensive and current overview of conventional DCBs-based nanosystems, stimuli-responsive DCBs-based nanosystems, and targeted DCBs-based nanosystems that have been reported in the literature for antibacterial delivery. The review emphasizes the DCBs used in their design, the nanomaterials constructed, the drug release-triggering stimuli, and the antibacterial efficacy of the reported DCBs-based nanosystems. Additionally, the review underlines future strategies that can be used to improve the potential of DCBs-based nanosystems to treat bacterial infections and overcome antibacterial resistance.

Antibiotic resistance is a pressing global health challenge, and polymyxins have emerged as the last line of defense against multidrug-resistant Gram-negative (MDR-GRN) bacterial infections. Despite the longstanding utility of colistin, the complexities surrounding polymyxins in terms of resistance mechanisms and pharmacological properties warrant critical attention. This review consolidates current literature, focusing on polymyxins antibacterial mechanisms, resistance pathways, and innovative strategies to mitigate resistance. We are also investigating the pharmacokinetics of polymyxins to elucidate factors that influence their in vivo behavior. A comprehensive understanding of these aspects is pivotal for developing next-generation antimicrobials and optimizing therapeutic regimens. We underscore the urgent need for advancing research on polymyxins to ensure their continued efficacy against formidable bacterial challenges.

Nanotechnology has advanced significantly, particularly in biomedicine, showing promise for nanomaterial applications. Bacterial infections pose persistent public health challenges due to the lack of rapid pathogen detection methods, resulting in antibiotic overuse and bacterial resistance, threatening the human microbiome. Nanotechnology offers a solution through nanoparticle-based materials facilitating early bacterial detection and combating resistance. This study explores recent research on nanoparticle development for controlling microbial infections using various nanotechnology-driven detection methods. These approaches include Surface Plasmon Resonance (SPR) Sensors, Surface-Enhanced Raman Scattering (SERS) Sensors, Optoelectronic-based sensors, Bacteriophage-Based Sensors, and nanotechnology-based aptasensors. These technologies provide precise bacteria detection, enabling targeted treatment and infection prevention. Integrating nanoparticles into detection approaches holds promise for enhancing patient outcomes and mitigating harmful bacteria spread in healthcare settings.