DNA extraction from archived hematoxylin and eosin-stained tissue slides for downstream molecular analysis

Table 1 Yield and purity of the recovered DNA from various methods

Method	Sample size, n	Tissue deparaffinization	Digestion period	Proteinase K inactivation	DNA yield range in ng/µL	DNA purity range, A260/280
1	20	Xylene	24 h	No	7.65-45.23	1.42-2.01
2	20	Xylene	24 h	Yes	7.53-33.7	1.34-1.94
3	20	Xylene	72 h	No	23.68-208.31	1.68-1.89
4	20	Xylene	72 h	Yes	13-149.41	1.54-1.93
5	20	Mineral oil	72 h	No	11.74-86.74	1.41-1.74

Table 2 Statistical comparison of the DNA yield among various methods

Sl No.	Unpaired t-test	P value	R value	Significance
1	Method 1 vs Method 2	0.908	0.0003	NS
2	Method 1 vs Method 3	< 0.0001	0.401	c
3	Method 1 vs Method 4	0.0017	0.23	b
4	Method 1 vs Method 5	0.524	0.01	NS
5	Method 2 vs Method 3	< 0.0001	0.418	c
6	Method 2 vs Method 4	0.0011	0.248	b
7	Method 2 vs Method 5	0.393	0.019	NS
8	Method 3 vs Method 4	0.13	0.059	NS
9	Method 3 vs Method 5	< 0.0001	0.366	c
10	Method 4 vs Method 5	0.004	0.192	b

P value < 0.05 was considered significant; ^bP < 0.01, ^cP < 0.001. NS: Not significant.