Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Methodol. Nov 14, 2019; 9(3): 32-43
Published online Nov 14, 2019. doi: 10.5662/wjm.v9.i3.32
DNA extraction from archived hematoxylin and eosin-stained tissue slides for downstream molecular analysis
Pushkal Sinduvadi Ramesh, Venkatesh Madegowda, Suprith Kumar, Shailashree Narasimha, Parichay S R, Nandini Nandish Manoli, Devananda Devegowda
Pushkal Sinduvadi Ramesh, Venkatesh Madegowda, Suprith Kumar, Shailashree Narasimha, Devananda Devegowda, Center of Excellence in Molecular Biology and Regenerative Medicine, Department of Biochemistry, JSS Medical College, JSS Academy of Higher Education and Research, Mysuru 570015, India
Parichay S R, CIPHER Healthcare Pvt Ltd., Hyderabad 500034, India
Nandini Nandish Manoli, Department of Pathology, JSS Medical College, JSS Academy of Higher Education and Research, Mysuru 570015, India
Author contributions: Ramesh PS, S R P, and Devegowda D conceptualized the study; Ramesh PS, Madegowda V, Kumar S, and Reddy S performed all of the experiments; Devegowda D and Manoli NN aided in collecting samples and providing resources; Ramesh PS and S R P wrote the original draft; Devegowda D and Ramesh PS wrote the final draft after editing.
Institutional review board statement: The Institutional Ethical Committee of JSS Medical College and Hospital, JSS Academy of Higher Education and Research, Mysuru approved the study. All research was conducted in accordance with the approved submission.
Conflict-of-interest statement: The authors have no conflicts of interest regarding the publication of this article.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Devananda Devegowda, MSc, PhD, Assistant Professor, Center of Excellence in Molecular Biology and Regenerative Medicine, Department of Biochemistry, JSS Medical College, JSS Academy of Higher Education and Research, Mysuru 570015, India. devanandd@jssuni.edu.in
Telephone: +91-961-1130458 Fax: +91-821-2548658
Received: August 5, 2019
Peer-review started: August 5, 2019
First decision: August 20, 2019
Revised: August 26, 2019
Accepted: October 15, 2019
Article in press: October 15, 2019
Published online: November 14, 2019
Processing time: 98 Days and 22.2 Hours
Core Tip

Core tip: In our study, we discussed a step-by-step procedure and results for the extraction of PCR-friendly DNA from archived hematoxylin and eosin-stained tissue slides. Such extracted DNA has the potential to be used for further molecular analyses such as mutation studies, whole genome sequencing, and even the identification of differences in gene signatures between diseased and normal states. Our protocol is simple, cost-effective, and can be performed in a basic molecular biology lab with most common reagents.