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©2014 Baishideng Publishing Group Inc.
World J Methodol. Dec 26, 2014; 4(4): 219-231
Published online Dec 26, 2014. doi: 10.5662/wjm.v4.i4.219
Published online Dec 26, 2014. doi: 10.5662/wjm.v4.i4.219
Table 1 Intra-assay imprecision
QC 1 | QC 2 | St 5 | Pool 1 | Pool 2 | |||||||||||||
Biomarker | Measuring | Unit | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc |
range | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | ||
CEA | 100-120000 | pg/mL | 2505 | 7.24 | 8.87 | 10933 | 2.32 | 3.39 | 2150 | 1.29 | 38897 | 2.29 | 3.98 | 466 | 6.77 | 7.78 | |
AFP | 500-600000 | pg/mL | 11980 | 3.81 | 4.23 | 54865 | 2.4 | 3.29 | 10703 | 2.88 | 455084 | 3.46 | 12.87 | 1598 | 4.99 | 11.99 | |
Total PSA | 50-60000 | pg/mL | 1230 | 5.97 | 7.9 | 5880 | 2.06 | 3.41 | 1057 | 1.92 | 7762 | 2.46 | 4.16 | < MR | < MR | < MR | |
CA 15-3 | 0.5-600 | U/mL | 12 | 9.1 | 8.43 | 57 | 4.06 | 4.5 | 11 | 3.05 | 29 | 6.48 | 6.35 | 24 | 3.87 | 3.7 | |
CA 19-9 | 2-3000 | U/mL | 61 | 9.54 | 7.35 | 292 | 5.27 | 7.69 | 54 | 3.2 | 33 | 3.35 | 2.66 | 10 | 1.65 | 1.75 | |
CA 125 | 2-4000 | U/mL | 71 | 6.77 | 6.68 | 347 | 4.14 | 4.4 | 68 | 2.27 | 302 | 4.15 | 4.36 | 5.53 | 6.23 | 11.25 | |
β-HCG | 0.2-400 | mU/mL | 8 | 8.86 | 5.54 | 39 | 5.56 | 4.25 | 7 | 1.54 | 0.65 | 6.37 | 36.09 | < MR | < MR | < MR | |
CYFRA 21-1 | 500-900000 | pg/mL | 29454 | 7.92 | 6.98 | 155151 | 3.7 | 3.18 | 16614 | 1.52 | 9724 | 4.22 | 4.77 | 3506 | 8.07 | 13.12 | |
HE4 | 2000-3000000 | pg/mL | 55648 | 8.73 | 6.98 | 291745 | 5.17 | 5.23 | 55123 | 4.1 | 2276 | 8.16 | 9.58 | < MR | < MR | < MR | |
Prolactin | 500-600000 | pg/mL | 11868 | 5.69 | 4.73 | 60195 | 3.04 | 3.55 | 10922 | 2.61 | 12057 | 4.37 | 3.69 | 10765 | 3.08 | 2.52 | |
Leptin | 500-600000 | pg/mL | 13056 | 8.97 | 5.05 | 62955 | 2.75 | 2.38 | 10971 | 1.94 | 13430 | 7.17 | 4.04 | 8468 | 2.78 | 1.48 | |
OPN | 2000-3000000 | pg/mL | 53895 | 8.94 | 7.32 | 236975 | 3.72 | 6.02 | 43171 | 0.91 | 73954 | 4.46 | 4.01 | 10870 | 2.75 | 2.25 | |
HGF | 100-120000 | pg/mL | 2452 | 9.76 | 8.64 | 12766 | 4.06 | 4.28 | 2154 | 2.86 | 785 | 3.11 | 2.86 | 283 | 5.65 | 7.44 | |
MIF | 100-120000 | pg/mL | 2230 | 7.83 | 5.97 | 11061 | 4.5 | 4.45 | 2209 | 3.06 | 574 | 1.89 | 1.68 | 513 | 14.31 | 15.66 | |
sFas | 100-150000 | pg/mL | 2486 | 6.3 | 5.53 | 12705 | 3.19 | 2.77 | 2779 | 2.4 | 3548 | 4.52 | 3.89 | 2217 | 1.67 | 1.47 | |
sFasL | 50-60000 | pg/mL | 1219 | 5.65 | 5.71 | 5898 | 2.17 | 2.28 | 1091 | 2.86 | < MR | < MR | < MR | < MR | < MR | < MR | |
TRAIL | 10-12000 | pg/mL | 252 | 7.23 | 7.41 | 1233 | 2.16 | 2.26 | 221 | 2.49 | 65 | 3.4 | 3.62 | 92 | 3.32 | 3.5 | |
VEGF | 50-60000 | pg/mL | 1127 | 13.04 | 8.8 | 6110 | 3.97 | 2.78 | 1115 | 4.41 | < MR | < MR | < MR | < MR | < MR | < MR | |
IL-6 | 2-3000 | pg/mL | 49 | 9.2 | 9.27 | 226 | 4.33 | 4.21 | 53 | 3.43 | 16 | 5.66 | 7.02 | < MR | < MR | < MR | |
IL-8 | 5-6000 | pg/mL | 122 | 5.53 | 5.67 | 605 | 2.19 | 2.34 | 110 | 1.71 | 33 | 5.85 | 6.7 | 6.71 | 4.49 | 9.43 | |
TNFα | 5-6000 | pg/mL | 114 | 7.77 | 7.97 | 562 | 2.88 | 3.17 | 110 | 2.25 | 13 | 4.56 | 5.66 | 6.41 | 3.12 | 6.68 | |
TGFα | 10-12000 | pg/mL | 237 | 6.36 | 6.29 | 1180 | 3.02 | 3.11 | 221 | 1.23 | 24 | 5.17 | 8.75 | 18 | 4.49 | 11.59 | |
FGF2 | 50-50000 | pg/mL | 1101 | 13.38 | 9.41 | 5571 | 2.61 | 2.85 | 1066 | 3.08 | 55 | 8.71 | 18.11 | 79 | 9.57 | 14.52 | |
SCF | 20-30000 | pg/mL | 599 | 8.41 | 7.97 | 2813 | 3.02 | 2.88 | 550 | 1.17 | 58 | 6.99 | 10.97 | 50 | 8.32 | 13.4 |
Table 2 Extended intra-assay imprecision
QC 1 | QC 2 | St 5 | Pool 1 | Pool 2 | |||||||||||||
Biomarker | Measuring | Unit | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc |
range | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | ||
CEA | 100-120000 | pg/mL | 2545 | 8.32 | 11.34 | 12727 | 7.61 | 12.23 | 2146 | 4.11 | 39391 | 2.1 | 3.63 | 560 | 8.09 | 5.67 | |
AFP | 500-600000 | pg/mL | 12038 | 6.09 | 6.9 | 56960 | 3.08 | 4.23 | 10754 | 4.45 | 493993 | 2.39 | 8.73 | 1489 | 6.87 | 11.8 | |
Total PSA | 50-60000 | pg/mL | 1421 | 5.76 | 7.78 | 6172 | 3.75 | 6.27 | 1052 | 4.1 | 8246 | 1.59 | 2.69 | 68 | 9.76 | 7.02 | |
CA 15-3 | 0.5-600 | U/mL | 12 | 9 | 8.7 | 62 | 4.18 | 4.96 | 11 | 4.32 | 33 | 5.31 | 5.23 | 26 | 9.19 | 8.66 | |
CA 19-9 | 2-3000 | U/mL | 64 | 10.63 | 8.63 | 329 | 4.83 | 7.1 | 54 | 5.05 | 36 | 2.9 | 2.39 | 10 | 4.87 | 6.17 | |
CA 125 | 2-4000 | U/mL | 74 | 7.02 | 7.03 | 370 | 3.61 | 3.78 | 68 | 2.84 | 317 | 3.59 | 3.64 | 6.13 | 4.74 | 8.54 | |
β-HCG | 0.2-400 | mU/mL | 7.85 | 10.62 | 7.02 | 42 | 4.89 | 3.67 | 7 | 5.08 | 1.2 | 6.76 | 18.28 | 0.55 | 8.17 | 31.39 | |
CYFRA 21-1 | 500-900000 | pg/mL | 24985 | 7.62 | 8.22 | 152731 | 12.13 | 4.28 | 16601 | 3.32 | 8627 | 4.34 | 5.73 | 3506 | 8.33 | 13.12 | |
HE4 | 2000-3000000 | pg/mL | 55766 | 11.42 | 9.39 | 295050 | 4.28 | 4.45 | 55043 | 1.9 | 2288 | 6.47 | 7.67 | 2590 | 11.67 | 8.86 | |
Prolactin | 500-600000 | pg/mL | 11952 | 7.86 | 6.96 | 60823 | 3.43 | 4.13 | 10863 | 4.49 | 12191 | 3.31 | 2.83 | 10602 | 6.1 | 4.71 | |
Leptin | 500-600000 | pg/mL | 13001 | 12.79 | 7.06 | 64533 | 2.79 | 2.4 | 11020 | 5.73 | 14622 | 5.67 | 3.18 | 9145 | 6.58 | 3.4 | |
OPN | 2000-3000000 | pg/mL | 57576 | 9.61 | 8.58 | 240688 | 4.93 | 9.29 | 42845 | 3.77 | 75488 | 3.61 | 3.35 | 10700 | 5.59 | 4.85 | |
HGF | 100-120000 | pg/mL | 2546 | 9.92 | 8.68 | 13471 | 3.23 | 3.3 | 2171 | 2.51 | 894 | 2.82 | 2.53 | 436 | 7.6 | 8.8 | |
MIF | 100-120000 | pg/mL | 2263 | 10.84 | 10.06 | 12332 | 5.12 | 5.99 | 2220 | 5.19 | 876 | 2.06 | 1.86 | 517 | 11.4 | 17.25 | |
sFas | 100-150000 | pg/mL | 2533 | 7.15 | 6.72 | 13212 | 3.91 | 3.47 | 2775 | 3.94 | 4005 | 3.54 | 3.07 | 2472 | 4.88 | 4.34 | |
sFasL | 50-60000 | pg/mL | 1245 | 6.35 | 6.49 | 6023 | 2.9 | 3.15 | 1093 | 5.13 | < MR | < MR | < MR | < MR | < MR | < MR | |
TRAIL | 10-12000 | pg/mL | 253 | 8.52 | 8.64 | 1269 | 2.65 | 2.72 | 220 | 3.73 | 72 | 4.22 | 4.71 | 98 | 6.19 | 6.44 | |
VEGF | 50-60000 | pg/mL | 1146 | 16.04 | 11.12 | 6155 | 4.61 | 3.56 | 1100 | 2.88 | < MR | < MR | < MR | <MR | < MR | < MR | |
IL-6 | 2-3000 | pg/mL | 54 | 11.72 | 12.01 | 268 | 6.35 | 6.11 | 54 | 1.79 | 21 | 6.25 | 7.44 | < MR | < MR | < MR | |
IL-8 | 5-6000 | pg/mL | 124 | 7.8 | 9.02 | 668 | 6.56 | 9.49 | 111 | 3.94 | 26 | 5.05 | 9.13 | 6.70 | 6.22 | 9.43 | |
TNFα | 5-6000 | pg/mL | 117 | 10.43 | 10.35 | 583 | 3.68 | 3.8 | 111 | 4.86 | 16 | 5.27 | 9.14 | 7.85 | 5.41 | 4.61 | |
TGFα | 10-12000 | pg/mL | 238 | 8.86 | 8.94 | 1210 | 3.78 | 4.05 | 221 | 4.91 | 27 | 3.99 | 9.09 | 18 | 5.32 | 9.69 | |
FGF2 | 50-50000 | pg/mL | 1104 | 20.18 | 14.17 | 5708 | 2.97 | 3.07 | 1068 | 2.04 | 68 | 9.63 | 13.35 | 80 | 7.72 | 7.97 | |
SCF | 20-30000 | pg/mL | 603 | 8.04 | 7.66 | 2951 | 4.39 | 4.32 | 549 | 3.96 | 79 | 6.49 | 8.93 | 59 | 8.5 | 15.12 |
Table 3 Inter-assay imprecision
QC 1 | QC 2 | St 5 | Pool 1 | Pool 2 | |||||||||||||
Biomarker | Measuring | Unit | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc |
range | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | ||
CEA | 100-120000 | pg/mL | 2505 | 10.45 | 3.92 | 10933 | 8.88 | 7.62 | 2150 | 11.53 | 4.15 | 38897 | 4.53 | 6.58 | 466 | 15.14 | 14.43 |
AFP | 500-600000 | pg/mL | 11980 | 13.92 | 5.32 | 54865 | 12.72 | 5.09 | 10703 | 12.14 | 3.43 | 455084 | 8.59 | 20.68 | 1598 | 34 | 66.04 |
Total PSA | 50-60000 | pg/mL | 1230 | 15.82 | 5.11 | 5880 | 11.51 | 11.21 | 1057 | 8.61 | 0.32 | 7762 | 7.88 | 4.59 | < MR | < MR | < MR |
CA 15-3 | 0.5-600 | U/mL | 12 | 18.57 | 6.38 | 57 | 21.29 | 2.85 | 11 | 20.52 | 1.79 | 29 | 16.48 | 8.48 | 24 | 27.78 | 21.2 |
CA 19-9 | 2-3000 | U/mL | 61 | 20.3 | 4.79 | 292 | 12.19 | 4.58 | 54 | 11.44 | 2.19 | 33 | 17.49 | 7.29 | 10 | 19.24 | 23.19 |
CA 125 | 2-4000 | U/mL | 71 | 13.39 | 3.23 | 347 | 11.19 | 3.9 | 68 | 11.73 | 1.91 | 302 | 6.26 | 8.27 | 5.53 | 22.25 | 36.3 |
β-HCG | 0.2-400 | mU/mL | 8 | 17.8 | 4.39 | 39 | 16.3 | 1.48 | 7 | 15.48 | 2.41 | 0.65 | 4.49 | 61.53 | < MR | < MR | < MR |
CYFRA 21-1 | 500-900000 | pg/mL | 29454 | 16.78 | 27.37 | 155151 | 15.64 | 40.96 | 16614 | 42.71 | 1.14 | 9724 | 38.02 | 50 | 3506 | 21.25 | 30.8 |
HE4 | 2000-3000000 | pg/mL | 55648 | 27.52 | 5.48 | 291745 | 23.45 | 3.46 | 55123 | 23.76 | 2.74 | 2276 | 14.67 | 63.85 | < MR | < MR | < MR |
Prolactin | 500-600000 | pg/mL | 11868 | 26.35 | 4.16 | 60195 | 15.98 | 2.69 | 10922 | 14.69 | 1.97 | 12057 | 21.22 | 10.08 | 10765 | 35.99 | 20.91 |
Leptin | 500-600000 | pg/mL | 13056 | 23.59 | 2.44 | 62955 | 14.97 | 3.92 | 10971 | 15.63 | 1.81 | 13430 | 18.98 | 8.79 | 8468 | 36.1 | 23.77 |
OPN | 2000-3000000 | pg/mL | 53895 | 45.75 | 4.01 | 236975 | 29.04 | 4.62 | 43171 | 31.41 | 2.38 | 73954 | 46.72 | 8.5 | 10870 | 85.54 | 22.7 |
HGF | 100-120000 | pg/mL | 2452 | 13.41 | 12.19 | 12766 | 16.96 | 8.28 | 2154 | 20.58 | 1.7 | 785 | 7.96 | 11.57 | 283 | 10.19 | 20.14 |
MIF | 100-120000 | pg/mL | 2230 | 19.69 | 7.89 | 11061 | 14.32 | 4.89 | 2209 | 12.31 | 1.68 | 574 | 8.59 | 13.68 | 513 | 63.56 | 62.9 |
sFas | 100-150000 | pg/mL | 2486 | 27.52 | 6.36 | 12705 | 21.86 | 6.83 | 2779 | 26.23 | 1.37 | 3548 | 30.07 | 7.78 | 2217 | 51.45 | 22.99 |
sFasL | 50-60000 | pg/mL | 1219 | 16.69 | 4.68 | 5898 | 20.4 | 2.92 | 1091 | 17.72 | 1.62 | < MR | < MR | < MR | < MR | < MR | < MR |
TRAIL | 10-12000 | pg/mL | 252 | 13.53 | 4.72 | 1233 | 13.51 | 4.09 | 221 | 15.17 | 1.85 | 65 | 10.05 | 9.26 | 92 | 27.2 | 23.98 |
VEGF | 50-60000 | pg/mL | 1127 | 25.4 | 4.27 | 6110 | 24.51 | 8.87 | 1115 | 18.99 | 3.76 | < MR | < MR | < MR | < MR | < MR | < MR |
IL-6 | 2-3000 | pg/mL | 49 | 14.49 | 9.34 | 226 | 22.21 | 17.12 | 53 | 18.81 | 4 | 16 | 12.3 | 14.64 | < MR | < MR | < MR |
IL-8 | 5-6000 | pg/mL | 122 | 8.85 | 5.21 | 605 | 11.32 | 3.73 | 110 | 9.31 | 1.98 | 33 | 9.31 | 8.37 | 6.71 | 25.35 | 40.54 |
TNFα | 5-6000 | pg/mL | 114 | 21.44 | 5.52 | 562 | 16.31 | 6.81 | 110 | 16.78 | 2.28 | 13 | 19.54 | 8.14 | 6.41 | 26.96 | 40.75 |
TGFα | 10-12000 | pg/mL | 237 | 16.15 | 6.26 | 1180 | 13.07 | 3.33 | 221 | 15.5 | 1.97 | 24 | 16.87 | 23.88 | 18 | 24.27 | 32.78 |
FGF2 | 50-50000 | pg/mL | 1101 | 11.17 | 8.98 | 5571 | 13.86 | 5.71 | 1066 | 12.75 | 3.89 | 55 | 11.36 | 68.81 | 79 | 19.8 | 40.04 |
SCF | 20-30000 | pg/mL | 599 | 15.96 | 10.08 | 2813 | 13.38 | 4.75 | 550 | 14.69 | 1.93 | 58 | 11.5 | 10.22 | 50 | 24.27 | 22.5 |
Table 4 Extended inter-assay imprecision
QC 1 | QC 2 | St 5 | Pool 1 | Pool 2 | |||||||||||||
Biomarker | Measuring | Unit | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc | Conc | FI | Conc |
range | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | Mean | Mean CV% | Mean CV% | ||
CEA | 100-120000 | pg/mL | 2545 | 13.96 | 28.08 | 12727 | 20.57 | 25.67 | 2146 | 13.34 | 4.59 | 39391 | 6.94 | 7.38 | 560 | 14.32 | 25.77 |
AFP | 500-600000 | pg/mL | 12038 | 23.54 | 20.99 | 56960 | 19.11 | 10.29 | 10754 | 21.43 | 3.25 | 493993 | 12.38 | 18.42 | 1489 | 33.13 | 63.64 |
Total PSA | 50-60000 | pg/mL | 1421 | 34.29 | 31.87 | 6172 | 17.44 | 13.78 | 1052 | 18.72 | 3.67 | 8246 | 16.03 | 12.46 | 68 | 130.09 | 151.66 |
CA 15-3 | 0.5-600 | U/mL | 12 | 30.11 | 18.58 | 62 | 29.25 | 12.98 | 11 | 34.01 | 2.93 | 33 | 23.72 | 17.26 | 26 | 30.49 | 27.59 |
CA 19-9 | 2-3000 | U/mL | 64 | 22.71 | 17.91 | 329 | 11.98 | 19.1 | 54 | 19.2 | 2.71 | 36 | 15.18 | 26.88 | 10 | 16.18 | 36.06 |
CA 125 | 2-4000 | U/mL | 74 | 14.42 | 22.04 | 370 | 12.5 | 11.07 | 68 | 17.29 | 2.42 | 317 | 7.72 | 12.62 | 6.13 | 18.13 | 37.03 |
β-HCG | 0.2-400 | mU/mL | 7.85 | 20.62 | 20.74 | 42 | 16.95 | 16.23 | 7 | 26.88 | 2.69 | 1.2 | 29.09 | 52.2 | 0.55 | 17.3 | 74.36 |
CYFRA 21-1 | 500-900000 | pg/mL | 24985 | 69.07 | 36.07 | 152731 | 81.41 | 52.97 | 16601 | 53.61 | 2.5 | 8627 | 50.45 | 55.77 | 3506 | 31.82 | 30.8 |
HE4 | 2000-3000000 | pg/mL | 55766 | 22.11 | 21.46 | 295050 | 19.92 | 10.63 | 55043 | 22.45 | 2.1 | 2288 | 19.7 | 68.01 | 2590 | 22.73 | 54.77 |
Prolactin | 500-600000 | pg/mL | 11952 | 23.76 | 21.49 | 60823 | 13.52 | 9.33 | 10863 | 14.81 | 2.05 | 12191 | 17.16 | 20.41 | 10602 | 29.43 | 28.46 |
Leptin | 500-600000 | pg/mL | 13001 | 29.19 | 15.47 | 64533 | 19.61 | 8.82 | 11020 | 23.3 | 1.88 | 14622 | 16.75 | 18.61 | 9145 | 31.06 | 27.03 |
OPN | 2000-3000000 | pg/mL | 57576 | 58.11 | 28.1 | 240688 | 24.08 | 13.88 | 42845 | 25.18 | 5.71 | 75488 | 41.77 | 25.01 | 10700 | 62.83 | 47.91 |
HGF | 100-120000 | pg/mL | 2546 | 15.19 | 20.7 | 13471 | 19.05 | 12.85 | 2171 | 22.65 | 2.04 | 894 | 7.18 | 27.83 | 436 | 29.6 | 49.81 |
MIF | 100-120000 | pg/mL | 2263 | 46.52 | 45.1 | 12332 | 20.48 | 18.05 | 2220 | 23.01 | 2.93 | 876 | 62.88 | 48.48 | 517 | 45.1 | 47.35 |
sFas | 100-150000 | pg/mL | 2533 | 27.15 | 18.62 | 13212 | 20.06 | 12.63 | 2775 | 27.38 | 1.77 | 4005 | 29.39 | 21.39 | 2472 | 39.44 | 28.26 |
sFasL | 50-60000 | pg/mL | 1245 | 19.09 | 20.91 | 6023 | 21.37 | 6.63 | 1093 | 22.37 | 1.98 | < MR | < MR | < MR | < MR | < MR | < MR |
TRAIL | 10-12000 | pg/mL | 253 | 20.17 | 21.78 | 1269 | 17.13 | 10.13 | 220 | 21.55 | 2.05 | 72 | 11.04 | 32.6 | 98 | 26.7 | 35.57 |
VEGF | 50-60000 | pg/mL | 1146 | 46.21 | 23 | 6155 | 45.49 | 8.97 | 1100 | 43.93 | 3.26 | < MR | < MR | < MR | < MR | < MR | < MR |
IL-6 | 2-3000 | pg/mL | 54 | 17.46 | 26.94 | 268 | 17.39 | 23.92 | 54 | 24.51 | 5.54 | 21 | 14.19 | 41.05 | < MR | < MR | < MR |
IL-8 | 5-6000 | pg/mL | 124 | 75.72 | 21.22 | 668 | 45.79 | 22.2 | 111 | 30.7 | 3.13 | 26 | 36.91 | 38.62 | 6.70 | 90.89 | 40.54 |
TNFα | 5-6000 | pg/mL | 117 | 18.6 | 23.55 | 583 | 16.6 | 11.16 | 111 | 19.41 | 2.52 | 16 | 15.5 | 28.1 | 7.85 | 22.35 | 49.24 |
TGFα | 10-12000 | pg/mL | 238 | 18.16 | 21.43 | 1210 | 19.49 | 7.74 | 221 | 22.29 | 2.56 | 27 | 17.64 | 45.12 | 18 | 19.22 | 48.85 |
FGF2 | 50-50000 | pg/mL | 1104 | 25.12 | 23.52 | 5708 | 17.67 | 9.9 | 1068 | 19.05 | 3.55 | 68 | 9.43 | 49.91 | 80 | 15.21 | 32.41 |
SCF | 20-30000 | pg/mL | 603 | 12.44 | 19.52 | 2951 | 16.66 | 11.26 | 549 | 17.88 | 2.01 | 79 | 31.9 | 40.75 | 59 | 22.35 | 32.17 |
- Citation: Hermann N, Dreßen K, Schildberg FA, Jakobs C, Holdenrieder S. Methodical and pre-analytical characteristics of a multiplex cancer biomarker immunoassay. World J Methodol 2014; 4(4): 219-231
- URL: https://www.wjgnet.com/2222-0682/full/v4/i4/219.htm
- DOI: https://dx.doi.org/10.5662/wjm.v4.i4.219