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World J Methodol. Dec 26, 2014; 4(4): 219-231
Published online Dec 26, 2014. doi: 10.5662/wjm.v4.i4.219
Published online Dec 26, 2014. doi: 10.5662/wjm.v4.i4.219
Methodical and pre-analytical characteristics of a multiplex cancer biomarker immunoassay
Natalie Hermann, Katja Dreßen, Christopher Jakobs, Stefan Holdenrieder, Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, 53127 Bonn, Germany
Frank A Schildberg, Institutes of Molecular Medicine and Experimental Immunology, University Hospital Bonn, 53127 Bonn, Germany
Author contributions: Hermann N and Dreßen K contributed equally; Hermann N, Dreßen K and Holdenrieder S designed the present study and coordinated the logistic process; Hermann N, Dreßen K and Holdenrieder S were responsible for defined blood sampling and storing; Hermann N, Dreßen K, Schildberg FA and Jakobs C were responsible for immunoassay measurements; Statistical analysis was performed by Hermann N and Dreßen K; Hermann N, Dreßen K and Holdenrieder S were involved in the interpretation of the data, the conception of the manuscript as well as the revision; all authors read and approved the final manuscript.
Correspondence to: Stefan Holdenrieder, MD, Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, Sigmund-Freud Str. 15, 53127 Bonn, Germany. stefan.holdenrieder@uni-bonn.de
Telephone: +49-228-28712126 Fax: +49-228-28712159
Received: December 2, 2013
Revised: September 11, 2014
Accepted: October 14, 2014
Published online: December 26, 2014
Processing time: 386 Days and 10.8 Hours
Revised: September 11, 2014
Accepted: October 14, 2014
Published online: December 26, 2014
Processing time: 386 Days and 10.8 Hours
Core Tip
Core tip: In this study, the methodological quality of a new research-use-only multiplex magnetic bead assay, particularly designed for cancer diagnosis, was evaluated. This attractive panel includes 24 biomarkers: established as well as auspicious tumor markers and markers deriving from the fields of apoptosis, immunology and angiogenesis. Herewith, the complexity and multifactorial background of a cancer disease is depicted. Measurements were performed with physiological serum pools and intra- and inter-assay imprecision as well as dilution linearity were assessed. Furthermore, the influence of preanalytical factors was investigated.