Editorial
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Methodol. Mar 26, 2016; 6(1): 20-24
Published online Mar 26, 2016. doi: 10.5662/wjm.v6.i1.20
Cation-exchange high-performance liquid chromatography for variant hemoglobins and HbF/A2: What must hematopathologists know about methodology?
Prashant Sharma, Reena Das
Prashant Sharma, Reena Das, Department of Hematology, Level 5, Research Block A, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India
Author contributions: Sharma P and Das R both made substantial contributions to conception of this article; Sharma P drafted the article; Das R made critical revisions related to important intellectual content; all authors gave final approval of the version of the article submitted.
Conflict-of-interest statement: Both authors of the paper declare that they have no conflicting interests (including commercial, personal, political, intellectual or religious interests) that are related to the work submitted for consideration for publication.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Prashant Sharma, MD, DNB, DM, Associate Professor, Department of Hematology, Level 5, Research Block A, Postgraduate Institute of Medical Education and Research, Sector 12, Chandigarh 160012, India. sharma.prashant@pgimer.edu.in
Telephone: +91-88-72016123
Received: August 28, 2015
Peer-review started: September 1, 2015
First decision: December 7, 2015
Revised: January 28, 2016
Accepted: February 23, 2016
Article in press: February 24, 2016
Published online: March 26, 2016
Processing time: 203 Days and 9.1 Hours
Core Tip

Core tip: Interpretation of cation-exchange high-performance liquid chromatography requires in-depth knowledge of the biological basics of the disorders of hemoglobin with knowledge of the technological aspects of the technique. Pathologists may be unaware of the nuances of the technique, the rigorous quality control required and the approach to pitfalls that may be encountered. Here we list the most common of these, and based on literature and our experience, attempt to guide novices in this exciting and useful technology.