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Mirica IC, Furtos G, Moldovan M, Prodan D, Petean I, Campian RS, Pall E, Lucaciu O. Morphology, Cytotoxicity, and Antimicrobial Activity of Electrospun Polycaprolactone Biomembranes with Gentamicin and Nano-Hydroxyapatite. MEMBRANES 2023; 14:10. [PMID: 38248701 PMCID: PMC10819002 DOI: 10.3390/membranes14010010] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/13/2023] [Revised: 12/12/2023] [Accepted: 12/20/2023] [Indexed: 01/23/2024]
Abstract
The aim of this research is to develop new nanocomposite membranes (NMs) for guided bone regeneration from polycaprolactone (PCL), with different concentrations of gentamicin sulfate (GEN) and nano-hydroxyapatite (nHAP) through electrospinning. The obtained NMs were characterized for structure through SEM and AFM, which revealed the influence of GEN and nHAP on the fiber diameter. The addition of GEN lowered the fiber diameter, and the addition of nHAP increased the diameter of the fibers. The NMs demonstrated antibacterial properties against P. aeruginosa, S. aureus, B. cereus, and E. coli depending on the drug concentration, while being negligibly affected by the nHAP content. NM cytotoxicity assessment, performed once using the MTT assay, revealed no cytotoxicity. The developed NMs could be a promising alternative for guided bone regeneration.
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Affiliation(s)
- Ioana-Codruta Mirica
- Department of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, 400347 Cluj-Napoca, Romania; (I.-C.M.); (R.-S.C.); (O.L.)
| | - Gabriel Furtos
- Department of Dental Materials, Raluca Ripan, Institute of Research in Chemistry, Babes-Bolyai University, 400294 Cluj-Napoca, Romania; (M.M.); (D.P.)
| | - Marioara Moldovan
- Department of Dental Materials, Raluca Ripan, Institute of Research in Chemistry, Babes-Bolyai University, 400294 Cluj-Napoca, Romania; (M.M.); (D.P.)
| | - Doina Prodan
- Department of Dental Materials, Raluca Ripan, Institute of Research in Chemistry, Babes-Bolyai University, 400294 Cluj-Napoca, Romania; (M.M.); (D.P.)
| | - Ioan Petean
- Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, 400294 Cluj-Napoca, Romania;
| | - Radu-Septimiu Campian
- Department of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, 400347 Cluj-Napoca, Romania; (I.-C.M.); (R.-S.C.); (O.L.)
| | - Emoke Pall
- Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania;
| | - Ondine Lucaciu
- Department of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, 400347 Cluj-Napoca, Romania; (I.-C.M.); (R.-S.C.); (O.L.)
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Xing H, Yang F, Sun S, Pan P, Wang H, Wang Y, Chen J. Green efficient ultrasonic-assisted extraction of abalone nacre water-soluble organic matrix for bioinspired enamel remineralization. Colloids Surf B Biointerfaces 2022; 212:112336. [PMID: 35051793 DOI: 10.1016/j.colsurfb.2022.112336] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/24/2021] [Revised: 12/31/2021] [Accepted: 01/12/2022] [Indexed: 11/25/2022]
Abstract
Reconstructing enamel-like hydroxyapatite structures on damaged teeth remains a great challenge in the materials science and dentistry due to its highly ordered hierarchical microstructure. Inspired by the mineralization of mollusk nacre in nature, abalone nacre water-soluble organic matrix (WSM) was isolated successfully though an ultrasonic-assisted water extraction (UWE) strategy with nondestructive activity and high-quality extraction for simulating the process of tooth hard tissue mineralization. Results showed that the UWE strategy significantly increased the protein yield from 7.60% to 9.60% and improved the polysaccharide yield from 2.59% to 3.34%, respectively, indicating its excellent extraction efficiency of WSM. Noteworthily, the smallest averaged particle size (~155 nm) of WSM were obtained at an ultrasound time of 6 h, whereas the highest absolute values (~ -32 mV) of zeta potential was produced. Moreover, it was proved that WSM could induce the growth of enamel-like hydroxyapatite crystals to further facilitate biomimetic remineralization of the demineralized enamel and restore its continuous and smooth surface structure in vitro. Besides, the hardness (4.37 ± 0.07 GPa) and modulus of elasticity (84.80 ± 1.49 GPa) of the WSM-repaired enamel was similar to that of native enamel, indicating superior mechanical properties after repair. Herein, it provides a promising green, efficient strategy for the remineralization of damaged enamel and high value utilization of waste abalone shells.
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Affiliation(s)
- Huaran Xing
- Marine College, Shandong University, Weihai 264209, China
| | - Faming Yang
- Marine College, Shandong University, Weihai 264209, China
| | - Shengjun Sun
- Shandong Provincial Key Laboratory of Oral Biomedicine, College of Stomatology, Shandong University, Jinan 250021, China.
| | - Panpan Pan
- Marine College, Shandong University, Weihai 264209, China; Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan 430022, China.
| | - Hongbo Wang
- Marine College, Shandong University, Weihai 264209, China
| | - Yuxin Wang
- Marine College, Shandong University, Weihai 264209, China
| | - Jingdi Chen
- Marine College, Shandong University, Weihai 264209, China; Shandong Laboratory of Advanced Materials and Green Manufacturing, Yantai 265599, China.
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Athanasiadou D, Carneiro KMM. DNA nanostructures as templates for biomineralization. Nat Rev Chem 2021; 5:93-108. [PMID: 37117611 DOI: 10.1038/s41570-020-00242-5] [Citation(s) in RCA: 43] [Impact Index Per Article: 10.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 11/26/2020] [Indexed: 12/22/2022]
Abstract
Nature uses extracellular matrix scaffolds to organize biominerals into hierarchical structures over various length scales. This has inspired the design of biomimetic mineralization scaffolds, with DNA nanostructures being among the most promising. DNA nanotechnology makes use of molecular recognition to controllably give 1D, 2D and 3D nanostructures. The control we have over these structures makes them attractive templates for the synthesis of mineralized tissues, such as bones and teeth. In this Review, we first summarize recent work on the crystallization processes and structural features of biominerals on the nanoscale. We then describe self-assembled DNA nanostructures and come to the intersection of these two themes: recent applications of DNA templates in nanoscale biomineralization, a crucial process to regenerate mineralized tissues.
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Mocanu A, Cadar O, Frangopol PT, Petean I, Tomoaia G, Paltinean GA, Racz CP, Horovitz O, Tomoaia-Cotisel M. Ion release from hydroxyapatite and substituted hydroxyapatites in different immersion liquids: in vitro experiments and theoretical modelling study. ROYAL SOCIETY OPEN SCIENCE 2021; 8:201785. [PMID: 33614097 PMCID: PMC7890514 DOI: 10.1098/rsos.201785] [Citation(s) in RCA: 22] [Impact Index Per Article: 5.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/08/2020] [Accepted: 11/20/2020] [Indexed: 05/06/2023]
Abstract
Multi-substituted hydroxyapatites (ms-HAPs) are currently gaining more consideration owing to their multifunctional properties and biomimetic structure, owning thus an enhanced biological potential in orthopaedic and dental applications. In this study, nano-hydroxyapatite (HAP) substituted with multiple cations (Sr2+, Mg2+ and Zn2+) for Ca2+ and anion ( Si O 4 4 - ) for P O 4 3 - and OH-, specifically HAPc-5%Sr and HAPc-10%Sr (where HAPc is HAP-1.5%Mg-0.2%Zn-0.2%Si), both lyophilized non-calcined and lyophilized calcined, were evaluated for their in vitro ions release. These nanomaterials were characterized by scanning electron microscopy, field emission-scanning electron microscopy and energy-dispersive X-ray, as well as by atomic force microscope images and by surface specific areas and porosity. Further, the release of cations and of phosphate anions were assessed from nano-HAP and ms-HAPs, both in water and in simulated body fluid, in static and simulated dynamic conditions, using inductively coupled plasma optical emission spectrometry. The release profiles were analysed and the influence of experimental conditions was determined for each of the six nanomaterials and for various periods of time. The pH of the samples soaked in the immersion liquids was also measured. The ion release mechanism was theoretically investigated using the Korsmeyer-Peppas model. The results indicated a mechanism principally based on diffusion and dissolution, with possible contribution of ion exchange. The surface of ms-HAP nanoparticles is more susceptible to dissolution into immersion liquids owing to the lattice strain provoked by simultaneous multi-substitution in HAP structure. According to the findings, it is rational to suggest that both materials HAPc-5%Sr and HAPc-10%Sr are bioactive and can be potential candidates in bone tissue regeneration.
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Affiliation(s)
- Aurora Mocanu
- Faculty of Chemistry and Chemical Engineering, Physical Chemistry Centre, Chemical Engineering Department, Babes-Bolyai University of Cluj-Napoca, 11 Arany J. Street, 400028 Cluj-Napoca, Romania
| | - Oana Cadar
- INCDO INOE 2000, Research Institute for Analytical Instrumentation, 67 Donath Street, 400293 Cluj-Napoca, Romania
| | - Petre T. Frangopol
- Faculty of Chemistry and Chemical Engineering, Physical Chemistry Centre, Chemical Engineering Department, Babes-Bolyai University of Cluj-Napoca, 11 Arany J. Street, 400028 Cluj-Napoca, Romania
| | - Ioan Petean
- Faculty of Chemistry and Chemical Engineering, Physical Chemistry Centre, Chemical Engineering Department, Babes-Bolyai University of Cluj-Napoca, 11 Arany J. Street, 400028 Cluj-Napoca, Romania
| | - Gheorghe Tomoaia
- Department of Orthopedics and Traumatology, Iuliu Hatieganu University of Medicine and Pharmacy, 400132 Cluj-Napoca, Romania
- Academy of Romanian Scientists, 54 Splaiul Independentei, 050094 Bucharest, Romania
| | - Gertrud-Alexandra Paltinean
- Faculty of Chemistry and Chemical Engineering, Physical Chemistry Centre, Chemical Engineering Department, Babes-Bolyai University of Cluj-Napoca, 11 Arany J. Street, 400028 Cluj-Napoca, Romania
| | - Csaba Pal Racz
- Faculty of Chemistry and Chemical Engineering, Physical Chemistry Centre, Chemical Engineering Department, Babes-Bolyai University of Cluj-Napoca, 11 Arany J. Street, 400028 Cluj-Napoca, Romania
| | - Ossi Horovitz
- Faculty of Chemistry and Chemical Engineering, Physical Chemistry Centre, Chemical Engineering Department, Babes-Bolyai University of Cluj-Napoca, 11 Arany J. Street, 400028 Cluj-Napoca, Romania
| | - Maria Tomoaia-Cotisel
- Faculty of Chemistry and Chemical Engineering, Physical Chemistry Centre, Chemical Engineering Department, Babes-Bolyai University of Cluj-Napoca, 11 Arany J. Street, 400028 Cluj-Napoca, Romania
- Academy of Romanian Scientists, 54 Splaiul Independentei, 050094 Bucharest, Romania
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Carmona FJ, Dal Sasso G, Bertolotti F, Ramírez-Rodríguez GB, Delgado-López JM, Pedersen JS, Masciocchi N, Guagliardi A. The role of nanoparticle structure and morphology in the dissolution kinetics and nutrient release of nitrate-doped calcium phosphate nanofertilizers. Sci Rep 2020; 10:12396. [PMID: 32709936 PMCID: PMC7382453 DOI: 10.1038/s41598-020-69279-2] [Citation(s) in RCA: 20] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/24/2020] [Accepted: 06/09/2020] [Indexed: 01/06/2023] Open
Abstract
Bio-inspired synthetic calcium phosphate (CaP) nanoparticles (NPs), mimicking the mineral component of bone and teeth, are emergent materials for sustainable applications in agriculture. These sparingly soluble salts show self-inhibiting dissolution processes in undersaturated aqueous media, the control at the molecular and nanoscale levels of which is not fully elucidated. Understanding the mechanisms of particle dissolution is highly relevant to the efficient delivery of macronutrients to the plants and crucial for developing a valuable synthesis-by-design approach. It has also implications in bone (de)mineralization processes. Herein, we shed light on the role of size, morphology and crystallinity in the dissolution behaviour of CaP NPs and on their nitrate doping for potential use as (P,N)-nanofertilizers. Spherical fully amorphous NPs and apatite-amorphous nanoplatelets (NPLs) in a core-crown arrangement are studied by combining forefront Small-Angle and Wide-Angle X-ray Total Scattering (SAXS and WAXTS) analyses. Ca2+ ion release rates differ for spherical NPs and NPLs demonstrating that morphology plays an active role in directing the dissolution kinetics. Amorphous NPs manifest a rapid loss of nitrates governed by surface-chemistry. NPLs show much slower release, paralleling that of Ca2+ ions, that supports both detectable nitrate incorporation in the apatite structure and dissolution from the core basal faces.
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Affiliation(s)
- Francisco J Carmona
- Department of Science and High Technology and To.Sca.Lab, University of Insubria, Via Valleggio 11, 22100, Como, Italy
| | - Gregorio Dal Sasso
- Institute of Crystallography and To.Sca.Lab, Consiglio Nazionale Delle Ricerche, Via Valleggio 11, 22100, Como, Italy
| | - Federica Bertolotti
- Department of Science and High Technology and To.Sca.Lab, University of Insubria, Via Valleggio 11, 22100, Como, Italy
| | - Gloria B Ramírez-Rodríguez
- Department of Science and High Technology and To.Sca.Lab, University of Insubria, Via Valleggio 11, 22100, Como, Italy
- Department of Inorganic Chemistry, University of Granada, Av. Fuentenueva S/N, 18071, Granada, Spain
| | - José M Delgado-López
- Department of Inorganic Chemistry, University of Granada, Av. Fuentenueva S/N, 18071, Granada, Spain
| | - Jan Skov Pedersen
- Department of Chemistry and Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000, Aarhus, Denmark
| | - Norberto Masciocchi
- Department of Science and High Technology and To.Sca.Lab, University of Insubria, Via Valleggio 11, 22100, Como, Italy.
| | - Antonietta Guagliardi
- Institute of Crystallography and To.Sca.Lab, Consiglio Nazionale Delle Ricerche, Via Valleggio 11, 22100, Como, Italy.
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Flash tooth whitening: A friendly formulation based on a nanoencapsulated reductant. Colloids Surf B Biointerfaces 2020; 195:111241. [PMID: 32679445 DOI: 10.1016/j.colsurfb.2020.111241] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/16/2020] [Revised: 06/12/2020] [Accepted: 07/02/2020] [Indexed: 11/23/2022]
Abstract
Tooth whitening materials have not undergone relevant advances in the last years. Current materials base their action on the oxidant activity of peroxides, which present the disadvantage of requiring long application times, along with unpleasant side effects of dental hypersensitivity (e.g. sharp pain). In this work, a novel tooth whitening formulation based on the encapsulation of a reducing agent (sodium metabisulfite) in liposomes is developed. An experimental design was applied to optimize the formulation in terms of whitening action and safety, using bovine teeth as in vitro model. Results were obtained by colorimetry, profilometry and nanoindentation techniques. The comparison with standard whitening treatments showed a similar whitening action of the optimized formulation but in remarkable shorter application times. Moreover, teeth roughness values obtained with the presented formulation conformed with ISO 28399. As mechanism of action, results obtained from fluorescent confocal microscopy showed the liposomal formulation to form a layer surrounding the enamel surface, enhancing the treatment efficacy in terms of diffusion of the protected reductant towards the enamel. The better efficiency of this formulation encourages its use as an alternative to current oxidative treatments.
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Biodegradable dendrimer functionalized carbon nanotube-hybrids for biomedical applications. JOURNAL OF POLYMER RESEARCH 2019. [DOI: 10.1007/s10965-019-1848-8] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 10/26/2022]
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Arumugam S, Ramamoorthy P, Chakkarapani LD. Synthesis and characterizations of biocompatible polymers and carbon nanotubes-based hybrids for biomedical applications. INT J POLYM MATER PO 2019. [DOI: 10.1080/00914037.2019.1616200] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/26/2022]
Affiliation(s)
- Sivaranjani Arumugam
- Department of Chemistry, Bharath Institute of Higher Education and Research, Chennai, India
| | | | - Lakshmi Devi Chakkarapani
- Laboratoired’ Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Sion, Switzerland
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Angelova Volponi A, Zaugg LK, Neves V, Liu Y, Sharpe PT. Tooth Repair and Regeneration. CURRENT ORAL HEALTH REPORTS 2018; 5:295-303. [PMID: 30524931 PMCID: PMC6244610 DOI: 10.1007/s40496-018-0196-9] [Citation(s) in RCA: 56] [Impact Index Per Article: 8.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Indexed: 01/09/2023]
Abstract
PURPOSE OF REVIEW Current dental treatments are based on conservative approaches, using inorganic materials and appliances.This report explores and discusses the newest achievements in the field of "regenerative dentistry," based on the concept of biological repair as an alternative to the current conservative approach. RECENT FINDINGS The review covers and critically analyzes three main approaches of tooth repair: the re-mineralization of the enamel, the biological repair of dentin, and whole tooth engineering. SUMMARY The development of a concept of biological repair based on the role of the Wnt signaling pathway in reparative dentin formation offers a new translational approach into development of future clinical dental treatments.In the field of bio-tooth engineering, the current focus of the researchers remains the establishment of odontogenic cell-sources that would be viable and easily accessible for future bio-tooth engineering.
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Affiliation(s)
- Ana Angelova Volponi
- Centre for Craniofacial and Regenerative Biology, Dental Institute, King’s College London, London, UK
| | - Lucia K. Zaugg
- Centre for Craniofacial and Regenerative Biology, Dental Institute, King’s College London, London, UK
- Department of Periodontology, Endodontology and Cariology, University Center for Dental Medicine Basel, University of Basel, Basel, Switzerland
| | - Vitor Neves
- Centre for Craniofacial and Regenerative Biology, Dental Institute, King’s College London, London, UK
| | - Yang Liu
- Centre for Craniofacial and Regenerative Biology, Dental Institute, King’s College London, London, UK
| | - Paul T. Sharpe
- Centre for Craniofacial and Regenerative Biology, Dental Institute, King’s College London, London, UK
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Shi X, Zhou K, Huang F, Zhang J, Wang C. Endocytic mechanisms and osteoinductive profile of hydroxyapatite nanoparticles in human umbilical cord Wharton's jelly-derived mesenchymal stem cells. Int J Nanomedicine 2018; 13:1457-1470. [PMID: 29559775 PMCID: PMC5856024 DOI: 10.2147/ijn.s155814] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/12/2022] Open
Abstract
Background As a potentially bioactive material, the widespread application of nanosized hydroxyapatite (nano-HAP) in the field of bone regeneration has increased the risk of human exposure. However, our understanding of the interaction between nano-HAP and stem cells implicated in bone repair remains incomplete. Methods Here, we characterized the adhesion and cellular internalization of HAP nanoparticles (HANPs) with different sizes (20 nm np20 and 80 nm np80) and highlighted the involved pathway in their uptake using human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hWJ-MSCs). In addition, the effects of HANPs on cell viability, apoptosis response, osteogenic differentiation, and underlying related mechanisms were explored. Results It was shown that both types of HANPs readily adhered to the cellular membrane and were transported into the cells compared to micro-sized HAP particles (m-HAP; 12 μm). Interestingly, the endocytic routes of np20 and np80 differed, although they exhibited similar kinetics of adhesion and uptake. Our study revealed involvement of clathrin- and caveolin-mediated endocytosis as well as macropinocytosis in the np20 uptake. However, for np80, clathrin-mediated endocytosis and some as-yet-unidentified important uptake routes play central roles in their internalization. HANPs displayed a higher preference to accumulate in the cytoplasm compared to m-HAP, and HANPs were not detected in the nucleolus. Exposure to np20 for 24 h caused a decrease in cell viability, while cells completely recovered with an exposure time of 72 h. Furthermore, HANPs did not influence apoptosis and necrosis of hWJ-MSCs. Strikingly, HANPs enhanced mRNA levels of osteoblast-related genes and stimulated calcium mineral deposition, and this directly correlated with the activation in c-Jun N-terminal kinases and p38 pathways. Conclusion Our data provide additional insight about the interactions of HANPs with MSCs and suggest their application potential in hard tissue regeneration.
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Affiliation(s)
- Xingxing Shi
- Department of Prosthodontics, Jiangsu Key Laboratory of Oral Diseases, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China
| | - Kai Zhou
- Department of Prosthodontics, Jiangsu Key Laboratory of Oral Diseases, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China
| | - Fei Huang
- Department of Prosthodontics, Jiangsu Key Laboratory of Oral Diseases, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China
| | - Juan Zhang
- Department of Prosthodontics, Jiangsu Key Laboratory of Oral Diseases, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China
| | - Chen Wang
- Department of Prosthodontics, Jiangsu Key Laboratory of Oral Diseases, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China
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Athanasiadou D, Jiang W, Goldbaum D, Saleem A, Basu K, Pacella MS, Böhm CF, Chromik RR, Hincke MT, Rodríguez-Navarro AB, Vali H, Wolf SE, Gray JJ, Bui KH, McKee MD. Nanostructure, osteopontin, and mechanical properties of calcitic avian eggshell. SCIENCE ADVANCES 2018; 4:eaar3219. [PMID: 29725615 PMCID: PMC5930395 DOI: 10.1126/sciadv.aar3219] [Citation(s) in RCA: 59] [Impact Index Per Article: 8.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/25/2017] [Accepted: 02/13/2018] [Indexed: 05/07/2023]
Abstract
Avian (and formerly dinosaur) eggshells form a hard, protective biomineralized chamber for embryonic growth-an evolutionary strategy that has existed for hundreds of millions of years. We show in the calcitic chicken eggshell how the mineral and organic phases organize hierarchically across different length scales and how variation in nanostructure across the shell thickness modifies its hardness, elastic modulus, and dissolution properties. We also show that the nanostructure changes during egg incubation, weakening the shell for chick hatching. Nanostructure and increased hardness were reproduced in synthetic calcite crystals grown in the presence of the prominent eggshell protein osteopontin. These results demonstrate the contribution of nanostructure to avian eggshell formation, mechanical properties, and dissolution.
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Affiliation(s)
| | - Wenge Jiang
- Faculty of Dentistry, McGill University, Montreal, Quebec H3A 0C7, Canada
| | - Dina Goldbaum
- Department of Mining and Materials Engineering, McGill University, Montreal, Quebec H3A 0C5, Canada
| | - Aroba Saleem
- Department of Mining and Materials Engineering, McGill University, Montreal, Quebec H3A 0C5, Canada
| | - Kaustuv Basu
- Facility for Electron Microscopy Research, McGill University, Montreal, Quebec H3A 0C7, Canada
| | - Michael S. Pacella
- Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
| | - Corinna F. Böhm
- Department of Materials Science and Engineering, Institute of Glass and Ceramics, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen 91058, Germany
| | - Richard R. Chromik
- Department of Mining and Materials Engineering, McGill University, Montreal, Quebec H3A 0C5, Canada
| | - Maxwell T. Hincke
- Department of Cellular and Molecular Medicine and Department of Innovation in Medical Education, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada
| | | | - Hojatollah Vali
- Facility for Electron Microscopy Research, McGill University, Montreal, Quebec H3A 0C7, Canada
- Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec H3A 0C7, Canada
| | - Stephan E. Wolf
- Department of Materials Science and Engineering, Institute of Glass and Ceramics, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen 91058, Germany
- Interdisciplinary Center for Functional Particle Systems, Friedrich-Alexander University Erlangen-Nürnberg, Haberstrasse 9a, Erlangen 91058, Germany
| | - Jeffrey J. Gray
- Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
- Program in Molecular Biophysics, Institute for Nanobiotechnology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21218, USA
| | - Khanh Huy Bui
- Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec H3A 0C7, Canada
| | - Marc D. McKee
- Faculty of Dentistry, McGill University, Montreal, Quebec H3A 0C7, Canada
- Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec H3A 0C7, Canada
- Corresponding author.
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Zhou Y, Zhou Y, Gao L, Wu C, Chang J. Synthesis of artificial dental enamel by an elastin-like polypeptide assisted biomimetic approach. J Mater Chem B 2018; 6:844-853. [DOI: 10.1039/c7tb02576a] [Citation(s) in RCA: 26] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/09/2023]
Abstract
The synthesized artificial enamel assisted by an elastin-like polypeptide revealed a similar structure and excellent mechanical properties to those of natural enamel.
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Affiliation(s)
- Yang Zhou
- State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of Ceramics
- Chinese Academy of Sciences
- Shanghai 200050
- P. R. China
- University of Chinese Academy of Sciences
| | - Yanling Zhou
- State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of Ceramics
- Chinese Academy of Sciences
- Shanghai 200050
- P. R. China
| | - Long Gao
- State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of Ceramics
- Chinese Academy of Sciences
- Shanghai 200050
- P. R. China
- University of Chinese Academy of Sciences
| | - Chengtie Wu
- State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of Ceramics
- Chinese Academy of Sciences
- Shanghai 200050
- P. R. China
| | - Jiang Chang
- State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of Ceramics
- Chinese Academy of Sciences
- Shanghai 200050
- P. R. China
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13
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Wang W, Yeung KWK. Bone grafts and biomaterials substitutes for bone defect repair: A review. Bioact Mater 2017; 2:224-247. [PMID: 29744432 PMCID: PMC5935655 DOI: 10.1016/j.bioactmat.2017.05.007] [Citation(s) in RCA: 954] [Impact Index Per Article: 119.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/18/2017] [Revised: 05/19/2017] [Accepted: 05/19/2017] [Indexed: 02/08/2023] Open
Abstract
Bone grafts have been predominated used to treat bone defects, delayed union or non-union, and spinal fusion in orthopaedic clinically for a period of time, despite the emergency of synthetic bone graft substitutes. Nevertheless, the integration of allogeneic grafts and synthetic substitutes with host bone was found jeopardized in long-term follow-up studies. Hence, the enhancement of osteointegration of these grafts and substitutes with host bone is considerably important. To address this problem, addition of various growth factors, such as bone morphogenetic proteins (BMPs), parathyroid hormone (PTH) and platelet rich plasma (PRP), into structural allografts and synthetic substitutes have been considered. Although clinical applications of these factors have exhibited good bone formation, their further application was limited due to high cost and potential adverse side effects. Alternatively, bioinorganic ions such as magnesium, strontium and zinc are considered as alternative of osteogenic biological factors. Hence, this paper aims to review the currently available bone grafts and bone substitutes as well as the biological and bio-inorganic factors for the treatments of bone defect.
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Affiliation(s)
- Wenhao Wang
- Department of Orthopaedics and Traumatology, The University of Hong Kong, Pokfulam, Hong Kong, China
- Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma, The University of Hong Kong Shenzhen Hospital, 1 Haiyuan 1st Road, Futian District, Shenzhen, China
| | - Kelvin W K Yeung
- Department of Orthopaedics and Traumatology, The University of Hong Kong, Pokfulam, Hong Kong, China
- Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma, The University of Hong Kong Shenzhen Hospital, 1 Haiyuan 1st Road, Futian District, Shenzhen, China
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Shou G, Dong L, Liu Z, Cheng K, Weng W. Facet-Specific Mineralization Behavior of Nano-CaP on Anatase Polyhedral Microcrystals. ACS Biomater Sci Eng 2017; 3:875-881. [PMID: 33429559 DOI: 10.1021/acsbiomaterials.7b00234] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/30/2022]
Abstract
Biomimetic mineralization of nanocalcium phosphate (CaP) on metal oxide surfaces has gained great interest because of their relevance to osseointegration performance of implant materials. However, precisely controlling the nucleation behavior of mineralized nano-CaP on metal oxide at selective sites still remains a challenge. Here, we demonstrate a phenomenon on facet-specific mineralization on anatase TiO2 polyhedral microcrystals organized by two facets of {101} and {001} in complete cell culture medium: nano-CaP covers up {101} facets, while there are a few on {001} facets. The comparative experimental results indicate that the preadsorbed fetal bovine serum (FBS) protein on {001} facets might play a barrier role in preventing sequential nucleation of nano-CaP. This work thus provides insight into the understanding of mineralization on metal oxides, and a way to control the mineralization.
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Affiliation(s)
- Guohui Shou
- School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China
| | - Lingqing Dong
- School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China
| | - Zongguang Liu
- School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China
| | - Kui Cheng
- School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China
| | - Wenjian Weng
- School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China
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Wolf SE, Böhm CF, Harris J, Demmert B, Jacob DE, Mondeshki M, Ruiz-Agudo E, Rodríguez-Navarro C. Nonclassical crystallization in vivo et in vitro (I): Process-structure-property relationships of nanogranular biominerals. J Struct Biol 2016; 196:244-259. [DOI: 10.1016/j.jsb.2016.07.016] [Citation(s) in RCA: 40] [Impact Index Per Article: 4.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/31/2016] [Revised: 05/25/2016] [Accepted: 07/22/2016] [Indexed: 12/20/2022]
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Conteduca F, Di Sette P, Iorio R, Caperna L, Argento G, Mazza D, Ferretti A. Nanohydroxyapatite promotes the healing process in open-wedge high tibial osteotomy: A CT study. Knee 2016; 23:763-9. [PMID: 27297937 DOI: 10.1016/j.knee.2016.04.011] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 06/07/2015] [Revised: 03/11/2016] [Accepted: 04/14/2016] [Indexed: 02/02/2023]
Abstract
BACKGROUND The aim of this prospective and randomized study was to evaluate the effectiveness of adding nanohydroxyapatite (NHA) to heterologous bone graft in open wedge high tibial osteotomy (OWHTO) by measuring the bone density of the tibial osteotomy gap. METHODS Twenty-seven patients (26 knees) were operated by OWHTO and randomly divided into two groups: pure graft group, in which the osteotomy gap was filled with only heterologous bone graft; nanohydroxyapatite group, in which the osteotomy gap was filled with heterologous bone graft and NHA. All patients underwent computed tomography (CT) examination within one week after operation (Time 0), and after two months (Time 1) and 12months (Time 2). CT volume acquired Hounsfield Units (HU) were calculated and the mean value of bone density on three planes was measured. RESULTS At Time 0, the mineral density of the nanohydroxyapatite group appeared significantly higher compared with the pure graft group, due to the presence of NHA. At Time 1, the mineral density of the nanohydroxyapatite group had decreased relative to Time 0, while in the pure graft group it remained unchanged. At Time 2, the mineral density in the nanohydroxyapatite group had further decreased, reaching values close to the mineral density of normal bone. In contrast, in the pure graft group the mineral density had increased, probably due to the lack of reabsorption of the graft and the development of sclerosis in the osteotomy borders. CONCLUSIONS The results of the present study show better osseointegration of the heterologous graft when nanohydroxyapatite is added.
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Affiliation(s)
- F Conteduca
- Orthopaedic Unit and Kirk Kilgour Sports Injury Centre, S. Andrea Hospital, University of Rome Sapienza, Italy
| | - P Di Sette
- Orthopaedic Unit and Kirk Kilgour Sports Injury Centre, S. Andrea Hospital, University of Rome Sapienza, Italy.
| | - R Iorio
- Orthopaedic Unit and Kirk Kilgour Sports Injury Centre, S. Andrea Hospital, University of Rome Sapienza, Italy
| | - L Caperna
- Orthopaedic Unit and Kirk Kilgour Sports Injury Centre, S. Andrea Hospital, University of Rome Sapienza, Italy
| | - G Argento
- Orthopaedic Unit and Kirk Kilgour Sports Injury Centre, S. Andrea Hospital, University of Rome Sapienza, Italy
| | - D Mazza
- Orthopaedic Unit and Kirk Kilgour Sports Injury Centre, S. Andrea Hospital, University of Rome Sapienza, Italy
| | - A Ferretti
- Orthopaedic Unit and Kirk Kilgour Sports Injury Centre, S. Andrea Hospital, University of Rome Sapienza, Italy
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Ajami S, Pakshir HR, Babanouri N. Impact of nanohydroxyapatite on enamel surface roughness and color change after orthodontic debonding. Prog Orthod 2016; 17:11. [PMID: 27004806 PMCID: PMC4826863 DOI: 10.1186/s40510-016-0124-2] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/11/2016] [Accepted: 03/01/2016] [Indexed: 11/22/2022] Open
Abstract
Background The aim of this prospective in vitro study was to evaluate the effect of nanohydroxyapatite (nanoHAP) serum on the enamel surface roughness and tooth color stability after orthodontic debonding procedure. Methods The crowns of 30 premolars were embedded in acrylic blocks with a 4 mm × 5-mm-sized window on the middle third of buccal surfaces. Primary roughness values were evaluated by an atomic force microscope (AFM). After bracket debonding, and polishing procedures, the second roughness parameters were recorded. Specimens were then randomly assigned to two equal groups. NanoHAP serum and HAP toothpaste were applied for 10 days in the first and second groups, respectively. Then, after the third AFM, initial color parameters were measured. Following 1-week immersion in the coffee solution, second color assessment was performed. The fourth AFM was registered after 2 months of aging process. Results All roughness parameters were elevated following debonding procedure. There was no statistically significant reduction in roughness parameters after 10 days of nanoHAP serum or HAP toothpaste application. Both groups showed significant color change after immersion in the coffee solution. Conclusions NanoHAP serum with the protocols used in this study could not restore enamel surfaces to their original condition.
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Affiliation(s)
- Shabnam Ajami
- Orthodontic Research Center, Shiraz University of Medical Science, Shiraz, Iran
| | - Hamid Reza Pakshir
- Orthodontic Research Center, Shiraz University of Medical Science, Shiraz, Iran
| | - Neda Babanouri
- Orthodontic Research Center, Shiraz University of Medical Science, Shiraz, Iran.
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Chien YC, Burwell AK, Saeki K, Fernandez-Martinez A, Pugach MK, Nonomura G, Habelitz S, Ho SP, Rapozo-Hilo M, Featherstone JD, Marshall SJ, Marshall GW. Distinct decalcification process of dentin by different cariogenic organic acids: Kinetics, ultrastructure and mechanical properties. Arch Oral Biol 2015; 63:93-105. [PMID: 26745819 DOI: 10.1016/j.archoralbio.2015.10.001] [Citation(s) in RCA: 28] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/22/2015] [Revised: 07/15/2015] [Accepted: 10/01/2015] [Indexed: 10/22/2022]
Abstract
OBJECTIVES We studied artificial dentin lesions in human teeth generated by lactate and acetate buffers (pH 5.0), the two most abundant acids in caries. The objective of this study was to determine differences in mechanical properties, mineral density profiles and ultrastructural variations of two different artificial lesions with the same approximate depth. METHODS 0.05M (pH 5.0) acetate or lactate buffer was used to create 1) 180μm-deep lesions in non-carious human dentin blocks (acetate 130h; lactate 14days); (2) demineralized, ∼180μm-thick non-carious dentin discs (3 weeks). We performed nanoindentation to determine mechanical properties across the hydrated lesions, and micro X-ray computed tomography (MicroXCT) to determine mineral profiles. Ultrastructure in lesions was analyzed by TEM/selected area electron diffraction (SAED). Demineralized dentin discs were analyzed by small angle X-ray scattering (SAXS). RESULTS Diffusion-dominated demineralization was shown based on the linearity between lesion depths versus the square root of exposure time in either solution, with faster kinetics in acetate buffer. Nanoindentation revealed lactate induced a significantly sharper transition in reduced elastic modulus across the lesions. MicroXCT showed lactate demineralized lesions had swelling and more disorganized matrix structure, whereas acetate lesions had abrupt X-ray absorption near the margin. At the ultrastructural level, TEM showed lactate was more effective in removing minerals from the collagenous matrix, which was confirmed by SAXS analysis. CONCLUSIONS These findings indicated the different acids yielded lesions with different characteristics that could influence lesion formation resulting in their distinct predominance in different caries activities, and these differences may impact strategies for dentin caries remineralization.
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Affiliation(s)
- Y-C Chien
- Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143-0758, US; Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, US
| | - A K Burwell
- Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143-0758, US
| | - K Saeki
- Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143-0758, US
| | - A Fernandez-Martinez
- Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, US; ISTerre, CNRS & University of Grenoble, B.P. 53X, Grenoble, Cedex 9, 38041, France
| | - M K Pugach
- Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143-0758, US
| | - G Nonomura
- Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143-0758, US
| | - S Habelitz
- Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143-0758, US
| | - S P Ho
- Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143-0758, US
| | - M Rapozo-Hilo
- Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143-0758, US
| | - J D Featherstone
- Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143-0758, US
| | - S J Marshall
- Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143-0758, US
| | - G W Marshall
- Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143-0758, US.
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Lee JI, Ryu JH. Pulsed laser ablation of hydroxyapatite in ethanol. JOURNAL OF THE KOREAN CRYSTAL GROWTH AND CRYSTAL TECHNOLOGY 2014. [DOI: 10.6111/jkcgct.2014.24.5.219] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/09/2022]
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Song J, Cheng H, Shen X, Hu J, Tong H. Characterization of calcium carbonate crystals in pigeon yolk sacs with different incubation times. Micron 2014; 60:39-48. [PMID: 24602270 DOI: 10.1016/j.micron.2014.01.003] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/29/2013] [Revised: 01/08/2014] [Accepted: 01/14/2014] [Indexed: 11/30/2022]
Abstract
Calcium carbonate crystals are known to form in the yolk sacs of fertile pigeon eggs at late stages of incubation. The composition and structure of these crystals were investigated, the crystallization environment was inspected, and the physical chemistry constants of the yolk fluid were determined through the incubation period. Polarized light microscopy was used to observe the generation and distribution of calcium carbonate crystals in the yolk sac. In addition, X-ray diffraction was employed to analyze the composition and crystal phase of the yolk sac. A decalcification and deproteination method was established to analyze the ultrastructure and composition of the crystals, as well as the internal relationship between inorganic and organic phases of the crystals. Additionally, scanning electron microscopy, transmission electron microscopy, X-ray energy dispersive spectroscopy, and Fourier transform infrared spectroscopy were used to evaluate the characteristics of the crystals. Our results demonstrated that the calcium carbonate crystals were mainly composed of vaterite and calcite, with vaterite being the major component. Vaterite, a type of biomaterial generated by an organic template control, presented as a concentric hierarchical spherical structure. The organic nature of the biomaterial prevented vaterite from transforming into calcite, which is more thermodynamically stable than vaterite. Additionally, the configuration, size, and aggregation of vaterite were also mediated by the organic template. This bio-vaterite was found during the incubation period and is valuable in calcium transport during embryonic development.
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Affiliation(s)
- Juan Song
- Key Laboratory of Analytical Chemistry for Biology and Medicine (Wuhan University), Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China; Analytical and Testing Center, Hubei University of Technology, Wuhan 430068, People's Republic of China
| | - Haixia Cheng
- Key Laboratory of Analytical Chemistry for Biology and Medicine (Wuhan University), Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China
| | - Xinyu Shen
- Key Laboratory of Analytical Chemistry for Biology and Medicine (Wuhan University), Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China
| | - Jingxiao Hu
- Key Laboratory of Analytical Chemistry for Biology and Medicine (Wuhan University), Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China
| | - Hua Tong
- Key Laboratory of Analytical Chemistry for Biology and Medicine (Wuhan University), Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
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Petrik M, Harbrecht B. Dissolution Kinetics of Nanocrystals. Chemphyschem 2013; 14:2403-6. [DOI: 10.1002/cphc.201300352] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/09/2013] [Indexed: 11/08/2022]
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Hulsart-Billström G, Xia W, Pankotai E, Weszl M, Carlsson E, Forster-Horváth C, Larsson S, Engqvist H, Lacza Z. Osteogenic potential of Sr-doped calcium phosphate hollow spheresin vitroandin vivo. J Biomed Mater Res A 2013; 101:2322-31. [DOI: 10.1002/jbm.a.34526] [Citation(s) in RCA: 18] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/28/2012] [Revised: 10/15/2012] [Accepted: 10/17/2012] [Indexed: 11/11/2022]
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Billström GH, Blom AW, Larsson S, Beswick AD. Application of scaffolds for bone regeneration strategies: current trends and future directions. Injury 2013; 44 Suppl 1:S28-33. [PMID: 23351866 DOI: 10.1016/s0020-1383(13)70007-x] [Citation(s) in RCA: 52] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 02/02/2023]
Abstract
Scaffolds are extensively used in surgery to replace missing bone and to achieve bony union and fusion. An ideal scaffold should not only maintain, induce, and restore biological functions where cells, extracellular matrix, and growth factors are needed, but also have the right properties with respect to degradation, cell binding, cellular uptake, non-immunogenicity, mechanical strength, and flexibility. Here we examine both the basic science behind the development of scaffolds and comprehensively and systematically review the clinical applications.
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Zhou C, Hong Y, Zhang X. Applications of nanostructured calcium phosphate in tissue engineering. Biomater Sci 2013; 1:1012-1028. [DOI: 10.1039/c3bm60058k] [Citation(s) in RCA: 40] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/08/2023]
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Heredia A, Colin-Garcia M, Peña-Rico MA, Beltrán LFLA, Grácio J, Contreras-Torres FF, Rodríguez-Galván A, Bucio L, Basiuk VA. Thermal, infrared spectroscopy and molecular modeling characterization of bone: An insight in the apatite-collagen type I interaction. ACTA ACUST UNITED AC 2013. [DOI: 10.4236/abc.2013.32027] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022]
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Okada M, Furuzono T. Hydroxylapatite nanoparticles: fabrication methods and medical applications. SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS 2012; 13:064103. [PMID: 27877527 PMCID: PMC5099760 DOI: 10.1088/1468-6996/13/6/064103] [Citation(s) in RCA: 46] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/16/2012] [Accepted: 11/19/2012] [Indexed: 05/30/2023]
Abstract
Hydroxylapatite (or hydroxyapatite, HAp) exhibits excellent biocompatibility with various kinds of cells and tissues, making it an ideal candidate for tissue engineering, orthopedic and dental applications. Nanosized materials offer improved performances compared with conventional materials due to their large surface-to-volume ratios. This review summarizes existing knowledge and recent progress in fabrication methods of nanosized (or nanostructured) HAp particles, as well as their recent applications in medical and dental fields. In section 1, we provide a brief overview of HAp and nanoparticles. In section 2, fabrication methods of HAp nanoparticles are described based on the particle formation mechanisms. Recent applications of HAp nanoparticles are summarized in section 3. The future perspectives in this active research area are given in section 4.
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Affiliation(s)
- Masahiro Okada
- Department of Biomaterials, Osaka Dental University, 8-1 Kuzuha-Hanazono, Hirakata, Osaka, 573-1121, Japan
| | - Tsutomu Furuzono
- Department of Biomedical Engineering, School of Biology-Oriented Science and Technology, Kinki University, 930 Nishi-Mitani, Kinokawa, Wakayama, 649-6493, Japan
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Swain S, Dorozhkin S, Sarkar D. Synthesis and dispersion of hydroxyapatite nanopowders. MATERIALS SCIENCE AND ENGINEERING: C 2012; 32:1237-1240. [DOI: 10.1016/j.msec.2012.03.014] [Citation(s) in RCA: 25] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/26/2022]
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Liu X, Zhao M, Lu J, Ma J, Wei J, Wei S. Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities. Int J Nanomedicine 2012; 7:1239-50. [PMID: 22419871 PMCID: PMC3299575 DOI: 10.2147/ijn.s28098] [Citation(s) in RCA: 62] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/07/2023] Open
Abstract
INTRODUCTION Hydroxyapatite (HA) is the principal inorganic constituent of human bone. Due to its good biocompatibility and osteoconductivity, all kinds of HA particles were prepared by different methods. Numerous reports demonstrated that the properties of HA affected its biological effects. METHODS Two kinds of nanohydroxyapatite with different sizes and crystallinities were obtained via a hydrothermal treatment method under different temperatures. It was found that at a temperature of 140°C, a rod-like crystal (n-HA1) with a diameter of 23 ± 5 nm, a length of 47 ± 14 nm, and crystallinity of 85% ± 5% was produced, while at a temperature of 80°C, a rod-like crystal (n-HA2) with a diameter of 16 ± 3 nm, a length of 40 ± 10 nm, and crystallinity of 65% ± 3% was produced. The influence of nanohydroxyapatite size and crystallinity on osteoblast viability was studied by MTT, scanning electron microscopy, and flow cytometry. RESULTS n-HA1 gave a better biological response than n-HA2 in promoting cell growth and inhibiting cell apoptosis, and also exhibited much more active cell morphology. Alkaline phosphatase activity for both n-HA2 and n-HA1 was obviously higher than for the control, and no significant difference was found between n-HA1 and n-HA2. The same trend was observed on Western blotting for expression of type I collagen and osteopontin. In addition, it was found by transmission electron microscopy that large quantities of n-HA2 entered into the cell and damaged the cellular morphology. Release of tumor necrosis factor alpha from n-HA2 was markedly higher than from n-HA1, indicating that n-HA2 might trigger a severe inflammatory response. CONCLUSION This work indicates that not all nanohydroxyapatite should be considered a good biomaterial in future clinical applications.
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Affiliation(s)
- Xiaochen Liu
- Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, People’s Republic of China
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Dorozhkin SV. Dissolution mechanism of calcium apatites in acids: A review of literature. World J Methodol 2012; 2:1-17. [PMID: 25237611 PMCID: PMC4145559 DOI: 10.5662/wjm.v2.i1.1] [Citation(s) in RCA: 97] [Impact Index Per Article: 7.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 09/23/2011] [Revised: 02/17/2012] [Accepted: 02/21/2012] [Indexed: 02/06/2023] Open
Abstract
Eight dissolution models of calcium apatites (both fluorapatite and hydroxyapatite) in acids were drawn from the published literature, analyzed and discussed. Major limitations and drawbacks of the models were conversed in details. The models were shown to deal with different aspects of apatite dissolution phenomenon and none of them was able to describe the dissolution process in general. Therefore, an attempt to combine the findings obtained by different researchers was performed which resulted in creation of the general description of apatite dissolution in acids. For this purpose, eight dissolution models were assumed to complement each other and provide the correct description of the specific aspects of apatite dissolution. The general description considers all possible dissolution stages involved and points out to some missing and unclear phenomena to be experimentally studied and verified in future. This creates a new methodological approach to investigate reaction mechanisms based on sets of affine data, obtained by various research groups under dissimilar experimental conditions.
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Li L, Mao C, Wang J, Xu X, Pan H, Deng Y, Gu X, Tang R. Bio-inspired enamel repair via Glu-directed assembly of apatite nanoparticles: an approach to biomaterials with optimal characteristics. ADVANCED MATERIALS (DEERFIELD BEACH, FLA.) 2011; 23:4695-701. [PMID: 21915920 DOI: 10.1002/adma.201102773] [Citation(s) in RCA: 83] [Impact Index Per Article: 5.9] [Reference Citation Analysis] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/19/2011] [Revised: 08/10/2011] [Indexed: 05/12/2023]
Affiliation(s)
- Li Li
- Centre of Biopathways and Biomaterials and Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang 310027, China
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Hulsart-Billström G, Hu Q, Bergman K, Jonsson KB, Åberg J, Tang R, Larsson S, Hilborn J. Calcium phosphates compounds in conjunction with hydrogel as carrier for BMP-2: a study on ectopic bone formation in rats. Acta Biomater 2011; 7:3042-9. [PMID: 21569871 DOI: 10.1016/j.actbio.2011.04.021] [Citation(s) in RCA: 53] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/05/2011] [Revised: 03/25/2011] [Accepted: 04/24/2011] [Indexed: 11/29/2022]
Abstract
Current treatment of fractures often involves the use of bone graft or bone morphogenetic proteins (BMP) to induce fracture healing, especially in patients with a compromised healing capacity. BMP has to be delivered in conjunction with a carrier. Unfortunately, there are drawbacks and limitations with current carriers, including their bovine origin which carries the risk of an immunological response. The physical properties also limit the use to open surgical procedures, as it cannot be injected. New carriers with improved properties are therefore needed. The aim of this study was to assess the ectopic bone forming capability of various calcium phosphate compounds when used in conjunction with a hydrogel as the carrier for BMP-2. Five different ceramic additives were tested, including β-tricalcium phosphate and four types of hydroxyapatite (HAP) (nanoHAP, HAP, clods of HAP >100 μm, and the biomimetic HAP Ostim35®). The compounds were injected into the thigh muscle of rats, where it formed a gel in situ. After 4 weeks bone formation was evaluated by peripheral quantitative computed tomography and histology. The major finding was that the 20 nm nanoHAP yielded a higher bone density than the other additives (P=0.0008, ANOVA with Tukey's multiple comparison test). We hypothesize that the higher bone density induced by nanoHAP might be due to nanocrystals of calcium phosphate acting as direct building blocks for biomineralization.
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Beniash E. Biominerals--hierarchical nanocomposites: the example of bone. WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY 2011; 3:47-69. [PMID: 20827739 DOI: 10.1002/wnan.105] [Citation(s) in RCA: 108] [Impact Index Per Article: 7.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/30/2022]
Abstract
Many organisms incorporate inorganic solids in their tissues to enhance their functional, primarily mechanical, properties. These mineralized tissues, also called biominerals, are unique organo-mineral nanocomposites, organized at several hierarchical levels, from nano- to macroscale. Unlike man-made composite materials, which often are simple physical blends of their components, the organic and inorganic phases in biominerals interface at the molecular level. Although these tissues are made of relatively weak components under ambient conditions, their hierarchical structural organization and intimate interactions between different elements lead to superior mechanical properties. Understanding basic principles of formation, structure, and functional properties of these tissues might lead to novel bioinspired strategies for material design and better treatments for diseases of the mineralized tissues. This review focuses on general principles of structural organization, formation, and functional properties of biominerals on the example the bone tissues.
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Affiliation(s)
- Elia Beniash
- Department of Oral Biology, University of Pittsburgh, Pittsburgh, PA, USA.
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33
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Wang L, Lu J, Xu F, Zhang F. Dynamics of crystallization and dissolution of calcium orthophosphates at the near-molecular level. ACTA ACUST UNITED AC 2011. [DOI: 10.1007/s11434-010-4184-2] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
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34
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Calcium phosphate mineralization with linear poly(ethylene imine): a time-resolved study. Colloid Polym Sci 2011. [DOI: 10.1007/s00396-011-2403-2] [Citation(s) in RCA: 13] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/18/2022]
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35
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Abstract
In order to understand the fundamental processes leading to biomineralization, this chapter focuses on the earliest events of homo/heterogeneous nucleation from an initial supersaturated solution phase and subsequent growth involving various possible precursor phases (amorphous or crystalline) to the final mineral phase by specific template and other influences. We also discuss how the combination of macroscopic constant composition and microscopic atomic force microscopy provides insights into the physical mechanisms of crystal growth and phase stability and the influences of proteins, peptides or other small molecules.Biodemineralization reactions of tooth enamel and bone may be inhibited or even suppressed when particle sizes fall into certain critical nanoscale levels. This phenomenon actually involves particle-size-dependent critical conditions of energetic control at the molecular level. Clearly, this dissolution termination is a kinetic phenomenon and cannot be attributed to reaction retardation as a result of surface modification by additives. Almost all biomineralized structures are highly hierarchical at many different length scales. At the lowest level they often consist of tiny crystals, typically tens to hundreds of nanometers. This size is not arbitrary; rather, it seems to give biominerals such as bone and tooth remarkable physical characteristics.
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36
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Meng Y, Qin YX, DiMasi E, Ba X, Rafailovich M, Pernodet N. Biomineralization of a self-assembled extracellular matrix for bone tissue engineering. Tissue Eng Part A 2009; 15:355-66. [PMID: 18759666 DOI: 10.1089/ten.tea.2007.0371] [Citation(s) in RCA: 18] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/30/2022] Open
Abstract
Understanding how biomineralization occurs in the extracellular matrix (ECM) of bone cells is crucial to the understanding of bone formation and the development of a successfully engineered bone tissue scaffold. It is still unclear how ECM mechanical properties affect protein-mineral interactions in early stages of bone mineralization. We investigated the longitudinal mineralization properties of MC3T3-E1 cells and the elastic modulus of their ECM using shear modulation force microscopy, synchrotron grazing incidence X-ray diffraction (GIXD), scanning electron microscopy, energy dispersive X-ray spectroscopy, and confocal laser scanning microscopy (CLSM). The elastic modulus of the ECM fibers underwent significant changes for the mineralizing cells, which were not observed in the nonmineralizing cells. On substrates conducive to ECM network production, the elastic modulus of mineralizing cells increased at time points corresponding to mineral production, whereas that of the nonmineralizing cells did not vary over time. The presence of hydroxyapatite in mineralizing cells and the absence thereof in the nonmineralizing ones were confirmed by GIXD, and CLSM showed that a restructuring of actin occurred only for mineral-producing cells. These results show that the correct and complete development of the ECM network is required for osteoblasts to mineralize. This in turn requires a suitably prepared synthetic substrate for bone development to succeed in vitro.
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Affiliation(s)
- Yizhi Meng
- Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794-2580, USA
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Prakash D, Behari J. Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity. Int J Nanomedicine 2009; 4:133-44. [PMID: 19774112 PMCID: PMC2747348 DOI: 10.2147/ijn.s5481] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/24/2009] [Indexed: 01/02/2023] Open
Abstract
The purpose of the present study was to use capacitive coupling of pulsed electromagnetic field (CC-PEMF) and hydroxyapatite nanoparticles (HAp) as a countermeasure to prevent osteoporosis induced by simulated microgravity. We used the hind-limb suspension (HLS) rat model to simulate microgravity-induced bone losses for 45 days. In order to compare the resulting changes, mineralogical (bone mineral density [BMD], calcium [Ca], and phosphorus [P]), biochemical (osteocalcin, alkaline phosphatase [ALP], and type I collagen), and histological (scanning electron microscopy) parameters were adopted. As a countermeasure to the above, the effect of PEMF and HAp application were examined. Three-month-old female Wistar rats were randomly divided into control (n = 8), HLS (n = 8), HLS with PEMF (n = 8), HLS with HAp nanoparticles (n = 8), and HLS with HAp and PEMF (n = 8). We observed: 1) significant decrease (p < 0.01) in BMD, Ca, P, type I collagen, and ALP activity in femur and tibia in hind-limb bone and serum osteocalcin in HLS rats as compared with the ground control. 2) Nonsignificant increase in BMD (p < 0.1), Ca (p < 0.1), P (p < 0.5), type I collagen (p < 0.1), and ALP activity (p < 0.5) in femur and tibia in hind-limb bone and serum osteocalcin (p < 0.5) in HLS + PEMF rats compared with HLS rats. 3) Significant increase in BMD (p < 0.02), Ca (p < 0.05), P (p < 0.05), type I collagen (p < 0.02), and ALP activity (p > 0.02) in femur and tibia in hind-limb bone with a nonsignificant increase in serum osteocalcin (p > 0.1) in HLS + HAp rats compared to HLS rats. 4) Significant increase in BMD (p > 0.01). Ca (p > 0.01). P (p > 0.01). type I collagen (p > 0.01). and ALP activity (p > 0.01) in femur and tibia in hind-limb bone and serum osteocalcin (p > 0.02) were also observed. Results suggest that a combination of low level PEMF and Hap nanoparticles has potential to control bone loss induced by simulated microgravity.
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Affiliation(s)
- D Prakash
- School of Environmental Sciences, Jawaharlal Nehru University, New Delhi--110067, India
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Shi Z, Huang X, Liu B, Tao H, Cai Y, Tang R. Biological response of osteosarcoma cells to size-controlled nanostructured hydroxyapatite. J Biomater Appl 2009; 25:19-37. [PMID: 19726533 DOI: 10.1177/0885328209339396] [Citation(s) in RCA: 38] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
Abstract
Osteosarcoma is a primary malignant bone tumor, most prevalent in children and adolescents, and is usually highly aggressive and eventually lethal. Despite multimodal therapies, there is no effective approach to treat this malignant disease. In this study, we observed the biological response of osteosarcoma cells to two kinds of hydroxyapatite nanoparticles (Nano HA), NanoHA-S and NanoHA-L. These nanospheres have the same crystallinity (phase) and morphology, but they differ in size. Cells treated with two kinds of Nano HA were inhibited and mainly led to apoptotic cell death. Caspase-9-dependent intrinsic apoptotic pathway plays a role. It was interesting that the suppression and the apoptosis of osteosarcoma cells was directly related to the size of nanoparticles and that the larger-sized Nano HA exhibited more effectiveness than the smaller one. This in vitro study suggested the potential of size-controlled calcium phosphate nanoparticles for use in therapeutic replacement and reconstruction of bone merits after osteosarcoma extraction.
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Affiliation(s)
- Zhongli Shi
- Department of Orthopaedic Surgery, The Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, China
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39
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Landfester K. Miniemulsion polymerization and the structure of polymer and hybrid nanoparticles. Angew Chem Int Ed Engl 2009; 48:4488-507. [PMID: 19455531 DOI: 10.1002/anie.200900723] [Citation(s) in RCA: 481] [Impact Index Per Article: 30.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/09/2022]
Abstract
The miniemulsion process allows the formation of complex structured polymeric nanoparticles and the encapsulation of a solid or liquid, an inorganic or organic, or a hydrophobic or hydrophilic material into a polymer shell. Many different materials, ranging from organic and inorganic pigments, magnetite, or other solid nanoparticles, to hydrophobic and hydrophilic liquids, such as fragrances, drugs, or photoinitators, can be encapsulated. Functionalization of the nanoparticles can also be easily obtained. Compared to polymerization processes in organic solvents, polymerization to obtain polymeric nanoparticles can be performed in environmentally friendly solvents, usually water.
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Affiliation(s)
- Katharina Landfester
- Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany.
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40
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Landfester K. Miniemulsionspolymerisation und Struktur von Polymer- und Hybridnanopartikeln. Angew Chem Int Ed Engl 2009. [DOI: 10.1002/ange.200900723] [Citation(s) in RCA: 43] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/06/2022]
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41
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Pouget EM, Bomans PHH, Goos JACM, Frederik PM, de With G, Sommerdijk NAJM. The initial stages of template-controlled CaCO3 formation revealed by cryo-TEM. Science 2009; 323:1455-8. [PMID: 19286549 DOI: 10.1126/science.1169434] [Citation(s) in RCA: 519] [Impact Index Per Article: 32.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/02/2022]
Abstract
Biogenic calcium carbonate forms the inorganic component of seashells, otoliths, and many marine skeletons, and its formation is directed by an ordered template of macromolecules. Classical nucleation theory considers crystal formation to occur from a critical nucleus formed by the assembly of ions from solution. Using cryotransmission electron microscopy, we found that template-directed calcium carbonate formation starts with the formation of prenucleation clusters. Their aggregation leads to the nucleation of amorphous nanoparticles in solution. These nanoparticles assemble at the template and, after reaching a critical size, develop dynamic crystalline domains, one of which is selectively stabilized by the template. Our findings have implications for template-directed mineral formation in biological as well as in synthetic systems.
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Affiliation(s)
- Emilie M Pouget
- Laboratory of Materials and Interface Chemistry, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, Netherlands
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42
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Wang L, Nancollas GH. Pathways to biomineralization and biodemineralization of calcium phosphates: the thermodynamic and kinetic controls. Dalton Trans 2009:2665-72. [PMID: 19333487 DOI: 10.1039/b815887h] [Citation(s) in RCA: 86] [Impact Index Per Article: 5.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
Abstract
Although extensive investigations of calcium phosphate crystallization have been performed, many have focused only on the final structures and morphologies and have not emphasized the need to consider the molecular contacts between mineral and matrix that drive nucleation nor the thermodynamic and kinetic controls imposed by matrix and soluble proteins during the nucleation stage. This review focuses on the earliest events of homo/heterogeneous nucleation from an initial supersaturated solution phase and subsequent growth. We also discuss how the combination of macroscopic constant composition (CC) and microscopic atomic force microscopy (AFM) provides insights into the physical mechanisms of crystal growth and phase stability and the influences of proteins, peptides or other small molecules. In addition, a new model for nanoscale enamel and bone demineralization suggests biodemineralization reactions may be inhibited or even suppressed when particle sizes fall into certain critical nanoscale levels. This size is not arbitrary; rather, it seems to give biominerals such as bones and teeth remarkable physical characteristics including self-preservation in the fluctuating physiological milieu.
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Affiliation(s)
- Lijun Wang
- Department of Chemistry, The State University of New York at Buffalo, Amherst, New York 14260, USA
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43
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Paparcone R, Kniep R, Brickmann J. Hierarchical pattern of microfibrils in a 3D fluorapatite–gelatine nanocomposite: simulation of a bio-related structure building process. Phys Chem Chem Phys 2009; 11:2186-94. [DOI: 10.1039/b817723f] [Citation(s) in RCA: 21] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
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44
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Ehrlich H, Koutsoukos PG, Demadis KD, Pokrovsky OS. Principles of demineralization: Modern strategies for the isolation of organic frameworks. Micron 2008; 39:1062-91. [DOI: 10.1016/j.micron.2008.02.004] [Citation(s) in RCA: 61] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/13/2007] [Revised: 02/08/2008] [Accepted: 02/10/2008] [Indexed: 11/16/2022]
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45
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Qiu SR, Orme CA. Dynamics of Biomineral Formation at the Near-Molecular Level. Chem Rev 2008; 108:4784-822. [DOI: 10.1021/cr800322u] [Citation(s) in RCA: 84] [Impact Index Per Article: 4.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/29/2022]
Affiliation(s)
- S. Roger Qiu
- Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, 7000 East Avenue, Mailstop L-367, Livermore, California 94550
| | - Christine A. Orme
- Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, 7000 East Avenue, Mailstop L-367, Livermore, California 94550
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46
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Affiliation(s)
- Lijun Wang
- Department of Chemistry, University at Buffalo, The State University of New York, Amherst, New York 14260
| | - George H. Nancollas
- Department of Chemistry, University at Buffalo, The State University of New York, Amherst, New York 14260
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47
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Zhang X, Li Z, Zhu XX. Biomimetic Mineralization Induced by Fibrils of Polymers Derived from a Bile Acid. Biomacromolecules 2008; 9:2309-14. [DOI: 10.1021/bm800204t] [Citation(s) in RCA: 14] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Affiliation(s)
- Xu Zhang
- Key Laboratory of Functional Polymer Materials, Institute of Polymer Chemistry, Nankai University, Tianjin, 300071, China, and Département de Chimie, Université de Montréal, C.P. 6128, Succ. Centre-ville, Montréal, QC, H3C 3J7, Canada
| | - Zhanyong Li
- Key Laboratory of Functional Polymer Materials, Institute of Polymer Chemistry, Nankai University, Tianjin, 300071, China, and Département de Chimie, Université de Montréal, C.P. 6128, Succ. Centre-ville, Montréal, QC, H3C 3J7, Canada
| | - X. X. Zhu
- Key Laboratory of Functional Polymer Materials, Institute of Polymer Chemistry, Nankai University, Tianjin, 300071, China, and Département de Chimie, Université de Montréal, C.P. 6128, Succ. Centre-ville, Montréal, QC, H3C 3J7, Canada
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48
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Ehrlich H, Koutsoukos PG, Demadis KD, Pokrovsky OS. Principles of demineralization: modern strategies for the isolation of organic frameworks. Part II. Decalcification. Micron 2008; 40:169-93. [PMID: 18804381 DOI: 10.1016/j.micron.2008.06.004] [Citation(s) in RCA: 54] [Impact Index Per Article: 3.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/08/2008] [Accepted: 06/30/2008] [Indexed: 12/15/2022]
Abstract
This is the second paper on principles of demineralization. The initial paper is dedicated to the common definitions and the history of demineralization. In present work we review the principles and mechanisms of decalcification, i.e., removing the mineral Ca-containing compounds (phosphates and carbonates) from the organic matrix in its two main aspects: natural and artificial. Natural chemical erosion of biominerals (cavitation of biogenic calcareous substrata by bacteria, fungi, algae, foraminifera, sponges, polychaetes, and mollusks) is driven by production of mineral and organic acids, acidic polysaccharides, and enzymes (cabonic anhydrase, alkaline and phosphoprotein phosphataes, and H(+)-ATPase). Examples of artifical decalcification includes demineralization of bone, dentin and enamel, and skeletal formations of corals and crustacean. The mechanism and kinetics of Ca-containing biomineral dissolution is analyzed within the framework of (i) diffusion-reaction theory; (ii) surface-reaction controlled, morphology-based theories, and (iii) phenomenological surface coordination models. The application of surface complexation model for describing and predicting the effect of organic ligands on calcium and magnesium dissolution kinetics is also described. Use of the electron microscopy-based methods for observation and visualization of the decalcification phenomenon is discussed.
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Affiliation(s)
- Hermann Ehrlich
- Max Bergmann Center of Biomaterials, Institute of Materials Science, Dresden University of Technology, Budapester Str. 27, D-01069 Dresden, Germany.
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Pan H, Tao J, Yu X, Fu L, Zhang J, Zeng X, Xu G, Tang R. Anisotropic demineralization and oriented assembly of hydroxyapatite crystals in enamel: smart structures of biominerals. J Phys Chem B 2008; 112:7162-5. [PMID: 18503266 DOI: 10.1021/jp802739f] [Citation(s) in RCA: 24] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
Abstract
It is interesting to note that the demineralization of natural enamel does not happen as readily as that of the synthesized hydroxyapatite (HAP), although they share a similar chemical composition. We suggest that the hierarchical structure of enamel is an important factor in the preservation of the natural material against dissolution. The anisotropic demineralization of HAP is revealed experimentally, and this phenomenon is understood by the different interfacial structures of HAP-water at the atomic level. It is found that HAP {001} facets can be more resistant against dissolution than {100} under acidic conditions. Although {100} is the largest surface of the typical HAP crystal, it is {001}, the smallest habit face, that is chosen by the living organisms to build the outer surface of enamel by an oriented assembly of the rodlike crystals. We reveal that such a biological construction can confer on enamel protections against erosion, since {001} is relatively dissolution-insensitive. Thus, the spontaneous dissolution of enamel surface can be retarded in biological milieu by such a smart construction. The current study demonstrates the importance of hierarchical structures in the functional biomaterials.
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Wang L, Guan X, Yin H, Moradian-Oldak J, Nancollas GH. Mimicking the Self-Organized Microstructure of Tooth Enamel. THE JOURNAL OF PHYSICAL CHEMISTRY. C, NANOMATERIALS AND INTERFACES 2008; 112:5892-5899. [PMID: 19169386 DOI: 10.1021/jp077105+] [Citation(s) in RCA: 48] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/17/2023]
Abstract
Under near-physiological pH, temperature, and ionic strength, amelogenin (Amel) accelerates hydroxyapatite (HAP) nucleation kinetics, decreasing the induction time in a concentration-dependent manner. Hierarchically organized apatite microstructures are achieved by self-assembly involving nucleated nanocrystallites and Amel oligomers and nanospheres at low supersaturations and protein concentrations in a slow and well-controlled constant composition (CC) system. The CC method allows the capture of an intermediate structure, the nanorod, following the formation of the critical nuclei at the earliest nucleation stages of calcium phosphate crystallization. The nanorod building blocks form spontaneously by synergistic interactions between flexible Amel protein assemblies and rigid calcium phosphate nanocrystallites. These intermediate structures further assemble by a self-epitaxial growth mechanism to form the final hierarchically organized microstructures that are compositionally and morphologically similar to natural enamel. This in vitro observation provides direct evidence that Amel promotes apatite crystallization and organization. We interpret our observations to propose that in vivo Amel may maximally exert an influence on the structural control of developing enamel crystals at the earliest nucleation stages.
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Affiliation(s)
- Lijun Wang
- Department of Chemistry, Natural Sciences Complex, University at Buffalo, The State University of New York, Amherst, New York 1426
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