Copyright
©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Transl Med. Aug 6, 2022; 10(2): 14-28
Published online Aug 6, 2022. doi: 10.5528/wjtm.v10.i2.14
Published online Aug 6, 2022. doi: 10.5528/wjtm.v10.i2.14
Cell-free mitochondrial DNA quantification in ischemic stroke patients for non-invasive and real-time monitoring of disease status
Nusrath Fathima, Sandeep Kumar Vishwakarma, Aleem Ahmed Khan, Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad 500058, Telangana, India
Sandhya Manorenj, Department of Neurology, Princess Esra Hospital, Deccan College of Medical Sciences, Hyderabad 500058, Telangana, India
Author contributions: Fathima N performed lab experiments, collected the data, performed the statistical analysis, and wrote and formatted the manuscript; Manorenj S provided samples and clinical inputs for the study; Khan AA and Vishwakarma SK provided inputs, designed the study, helped in data analysis and presentation, and wrote and edited the manuscript.
Institutional review board statement: All study procedures were carried out with the approval of the Institutional Review Board of Deccan College of Medical Sciences, Hyderabad, Telangana, India.
Institutional animal care and use committee statement: All animal experiments conformed to the internationally accepted principles for the care and use of laboratory animals.
Conflict-of-interest statement: All authors declare that they have no conflict of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: All authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Aleem Ahmed Khan, PhD, Senior Scientist, Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad 500058, Telangana, India.
Received: April 2, 2022
Peer-review started: April 2, 2022
First decision: May 31, 2022
Revised: June 14, 2022
Accepted: July 16, 2022
Article in press: July 16, 2022
Published online: August 6, 2022
Processing time: 122 Days and 16 Hours
Peer-review started: April 2, 2022
First decision: May 31, 2022
Revised: June 14, 2022
Accepted: July 16, 2022
Article in press: July 16, 2022
Published online: August 6, 2022
Processing time: 122 Days and 16 Hours
Core Tip
Core Tip: Several blood biomarkers have been studied to determine the severity and outcome of ischemic stroke with limited applications. Hence, we need to establish molecular markers which can provide more comprehensive information on the stroke pathophysiology and treatment response. Dynamic quantification of plasma cell-free DNA appears to be a valid and reliable option. Hence, we compared the real-time expression of cell-free mitochondrial DNA (NADH-ubiquinone oxidoreductase chain 1 gene) in ischemic stroke patients with healthy controls and studies its prognostic value during the treatment. This study could aid in the development of clinical values for assessing real-time, non-invasive mode of ischemic stroke status in the future.