Rapid genotyping of human rotavirus using SYBR green real-time reverse transcription-polymerase chain reaction with melting curve analysis

Table 1 Polymerase chain reaction primers used for G and P typing of human rotavirus by reverse transcriptase polymerase chain reaction

Primer	Sequence (5’-3’)	Amplicon size (bp)	Direction	Ref.
G-type
VP7-R	AAC TTG CCA CCA TTT TTT CC		Antisense	Iturriza-Gómara et al[10]
G1	CAA GTA CTC AAA TCA ATG ATG G	618	Sense	Gouvea et al[8]
G2	CAA TGA TAT TAA CAC ATT TTC TGT G	521	Sense	Gouvea et al[8]
G3	ACG AAC TCA ACA CGA GAG G	682	Sense	Iturriza-Gómara et al[10]
G4	CGT TTC TGG TGA GGA GTT G	452	Sense	Gouvea et al[8]
G8	TTR1 TCG CAC CAT TTG TAA TT	756	Sense	Aladin et al[12]
G9	CTT GAT GTG ACT AY1A AAT AC	179	Sense	Iturriza-Gómara et al[10]
G10	ATG TCA GAC TAC AR2A TAC TGG	266	Sense	Gouvea et al[8]
G12	GGT TAT GTA ATC CGA TGG CG	396	Sense	Aladin et al[12]
P-type
VP4-F	TAT GCT CCA GTN3 AAT TGG		Sense	Simmonds et al[11]
P[4]	CTA TTG TTA GAG GTT AGA GTC	362	Antisense	Gentsch et al[9]
P[6]	TGT TGA TTA GTT GGA TTC AA	146	Antisense	Gentsch et al[9]
P[8]	TCT ACT GGR2 TTR2 ACN3 TGC	224	Antisense	Iturriza-Gómara et al[10]
P[9]	TGA GAC ATG CAA TTG GAC	270	Antisense	Gentsch et al[9]
P[10]	ATC ATA GTT AGT AGT CGG	462	Antisense	Gentsch et al[9]
P[11]	GTA AAC ATC CAG AAT GTG	191	Antisense	Iturriza-Gómara et al[10]

¹Y: C or T;

²R: A or G;

³N: A, G, C or T.