Searching for nuclear export elements in hepatitis D virus RNA

Table 1 Primers used to amplify hepatitis delta virus cDNA fragments

HDV cDNA fragments	Forward primer 5'→3'	Reverse primer 5'→3'	Fragment length (bp)	Genomelocation(Sense)	Antigenomelocation(Antisense)
A1	CGCATCGATACTCCCTGCAGATTGGGGA	CGCATCGATATTCACCGACAAGGAGAGGC	243	360-602	1078-1320
A2	CGCATCGATGCCTCTCCTTGTCGGTGAAT	CGCATCGATGAGACCTCCGGAAGACAAAGA	204	176-379	1301-1504
A3	CGCATCGATTTCCGGAGGTCTCTCTCGAGT	CGCATCGATTCTCCTCGCTCGGAACTTG	214	1654-679/188	1492-1679/26
A4	CGCATCGATTTCCTCGGTCAACCTCCTGA	CGCATCGATATAAGGATGGAGAGGGGGCT	224	266-489	1191-1414
A5	CGCATCGATCCCCTCTCCATCCTTATCCT	CGCATCGATAGGGAGAGAAGAGATCCTCGA	172	114-285	1395-1566
A6	CGCATCGATCGAATGGGACCCACAAATCT	CGCATCGATTCCCCAATCTGCAGGGAGT	337	584-920	760-1096
A7	CGCATCGATCCCAATCCCAGATCTGGAGA	CGCATCGATTTTGCTTTCCTCCTCGCTTC	204	1263-1466	214-417
A8	CGCATCGATAAAGAAAGCAACGGGGCTAG	CGCATCGATGGGAGTCGGAATCGAGCAT	199	1067-1265	415-613
A9	CGCATCGATATGCTCGATTCCGACTCCC	CGCATCGATCCTAGAGAGATTTGTGGGTCCC	191	895-1085	595-785
A10	CGCATCGATCAAGTTCCGAGCGAGGAGAC	CGCATCGATTCTCCAGATCTGGGATTGGG	227	1446-1672	8-234

Nucleotide sequence of the primers used in PCR reactions to amplify hepatitis delta virus (HDV) cDNA fragments. The ClaI restriction site is underlined. The size of the amplicon and the corresponding location in the genome, when cloned in sense and antisense orientations, are indicated.

Table 2 Primers used to amplify truncated forms of the A7AS agRNA sequence

HDV cDNA fragments	Forward primer 5'→3'	Reverse primer 5'→3'	Fragment length (bp)
269-417	CGCATCGATGGGAGGAATCCACTCGGAGA	CGCATCGATTTTGCTTTCCTCCTCGCTTC	149
214-379	CGCATCGATCCCAATCCCAGATCTGGAGA	CGCATCGATGCATCTCCTCCTATCGCTATGG	166
214-403	CGCATCGATCCCAATCCCAGATCTGGAGA	CGCATCGATGCTTCGGTCTCCCCCTACTC	190
244-417	CGCATCGATCCCGAAGGGTTGAGTAGCAC	CGCATCGATTTTGCTTTCCTCCTCGCTTC	174
244-403	CGCATCGATCCCGAAGGGTTGAGTAGCAC	CGCATCGATGCTTCGGTCTCCCCCTACTC	160
314-417	CGCATCGATACCCCTTCAGCGAACAAGAG	CGCATCGATTTTGCTTTCCTCCTCGCTTC	104
214-322	CGCATCGATCCCAATCCCAGATCTGGAGA	CGCATCGATTGAAGGGGTCCTCGGAGGT	109
269-379	CGCATCGATGGGAGGAATCCACTCGGAGA	CGCATCGATGCATCTCCTCCTATCGCTATGG	111

Nucleotide sequence of the primers used to amplify eight truncated forms of the A7AS sequence localized in position 214-417 in the hepatitis delta virus (HDV) antigenomic cDNA. The underlined sequence corresponds to a ClaI restriction site.