Emergence of Toscana virus in the mediterranean area

Table 1 Reverse transcriptase polymerase chain reaction systems described in the literature for Toscana virus

Name	Sequence	Gene	Assay	Ref.
TV1	CCAGAGGCCATGATGAAGAAGAT	N	RT-PCR	[56]
TV2	CCACTCCTATGAGCAGCTTCT	N	RT-PCR
TV3	AACCTGATTTCAGTCTACCAGTT	N	Nested
TV4	TTGTTCTCAGAGATGGATTTATG	N	Nested
TosN123	GAGTTTGCTTACCAAGGGTTTG	N	RT-PCR	[37]
TosN829	AATCCTAATTCCCCTAACCCCC	N	RT-PCR
TosN234	AACCTTGTCAGGGGNAACAAGCC	N	Nested
TosN794	GCCAACCTTGGCGCGATACTTC	N	Nested
NPhlebo1+	ATGGARGGITTTGTIWSICIICC	L	RT-PCR	[37]
Nphlebo1-	AARTTRCTIGWIGCYTTIARIGTIGC	L	RT-PCR
Nphlebo2+	WTICCIAAICCIYMSAARATG	L	Nested
Nphlebo2-	TCYTCYTTRTTYTTRARRTARCC	L	Nested
ATos2-	RTGRAGCTGGAAKGGIGWIG	L	Nested
TosS1+	CAGAGATTCCCGTGTATTAAAC	N	Nested	[58]
TosS1-	GAGTGCTGCCAAGTCTTATGAC	N	Nested
TosS2+	CAGAGATTCCCGTGTATTAAACAAAAGC	N	Nested
TosS2-	TAGAGAAACTGCTCTTTCCACC	N	Nested
T1	CTATCAACATGTCAGACGAG	N	RT-PCR	[56]
T2	CGTGTCCTGTCAGAATCCCT	N	RT-PCR
T3	CATTGTTCAGTTGGTCAA	N	Nested
T4	CGTGTCCTGTCAGAATCCCT	N	Nested
Phlebo F1	TTTGCTTATCAAGGATTTGATGC	N	RT-PCR	[65]
Phlebo F2	TTTGCTTATCAAGGATTTGACC	N	RT-PCR
Phlebo rev	TCAATCAGTCCAGCAAAGCTGGGATGCATCAT	N	RT-PCR
SFNV-S1	CTTYTTRTCYTCYCTRGTGAAGAA	N	RT-PCR	[64]
SFNV-R1	ATGATGAAGAARATGTCAGAGAA	N	RT-PCR
SFNV-S2	GCRGCCATRTTKGGYTTTTCAAA	N	Nested
SFNV-R2	CCTGGCAGRGACACYATCAC	N	Nested
STOS-50F	TGCTTTTCTTGATGAGTCTGCAG		rt RT-PCR	[59]
STOS-138R	CAATGCGCTTYGGRTCAAA		rt RT-PCR
STOS-84T-FAM	ATCAATGCATGGGTRAATGAGTTTGCTTACC		rt RT-PCR
TOS FP	GGGTGCATCATGGCTCTT		rt RT-PCR	[60]
TOS P	CAATGGCATCCATAGTGGTCCCAGA		rt RT-PCR
TOS RP	GCAGRGACACCATCACTCTGTC		rt RT-PCR