Rapid genotyping of human rotavirus using SYBR green real-time reverse transcription-polymerase chain reaction with melting curve analysis

Figure 1 Melting cure analysis of different genotypes using reverse transcriptase polymerase chain reaction (A: G-typing: G1, G2, G4, G9, G12, G3; B: P-typing: P[8], P[4]). The peak of curve in X-axis is the melting temperature of each genotype DNA fragment; Y-axis indicates the SYB green fluorescence density.

Figure 2 Melting temperatures of different rotavirus genotypes using reverse transcriptase polymerase chain reaction. The mean T_m (°C ± SD) for each genotype (G1 to G4, G9, G12, P[4] and P[8]) were calculated from more than 20 replicates of different samples at different polymerase chain reaction runs.

Figure 3 Agarose gel electrophoresis of different rotavirus genotype using reverse transcriptase polymerase chain reaction. Amplicon sizes for different genotypes (G1: 618 bp; G2: 521 bp; G3: 682 bp; G4: 452 bp; G9: 179 bp; G12: 396 bp; P[4]: 362 bp; P[8]: 224 bp. M: 100 bp DNA ladder (life technologies).