Detection of dengue virus encephalitis in central part of Sri Lanka

Figure 1 Map of Sri Lanka highlighting the districts of the central province (Matale, Kandy, and Nuwara Eliya). Colors represent the three districts of the central province as indicated in the legend.

Figure 2 Gel electrophoresis image showing reverse transcriptase polymerase chain reaction amplification of cerebrospinal fluid samples using flavivirus-universal primers. The amplified products were visualized with Ethidium bromide staining. L: 100 bp DNA ladder; PC: Positive control (Japanese encephalitis virus); NC: Negative control; Sample ID 3925: A patient sample showing a distinct band between 500-600 bp; Other lanes: Non-reactive cerebrospinal fluid samples. Arrow: Positive control in par with 500 pb of the ladder.

Figure 3  Real-time reverse transcriptase polymerase chain reaction amplification curve of the positive cerebrospinal fluid sample (ID: 3925).