Observational Study
Copyright ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Transplant. Aug 9, 2018; 8(4): 110-121
Published online Aug 9, 2018. doi: 10.5500/wjt.v8.i4.110
Renal transplants from older deceased donors: Is pre-implantation biopsy useful? A monocentric observational clinical study
Giacomo Colussi, Costanza Casati, Valeriana Giuseppina Colombo, Mario Livio Pietro Camozzi, Fabio Rosario Salerno
Giacomo Colussi, Costanza Casati, Valeriana Giuseppina Colombo, Fabio Rosario Salereno, Division of Nephrology, Dialysis and Renal Transplantatation, ASST Grande Ospedale Territoriale Niguarda, Milan 20162, Italy
Mario Livio Pietro Camozzi, Division of Pathology, ASST Grande Ospedale Territoriale Niguarda, Milan 20162, Italy
Fabio Rosario Salerno, School of Nephrology, Milano-Bicocca University, Milan 20126, Italy
Author contributions: Colussi G designed the study, wrote the draft, and contributed to data acquisition, data analysis and interpretation; Camozzi MPL, Colombo VG and Casati C contributed to data acquisition, editing, reviewing and final approval of article; Salerno FR contributed to data acquisition and data analysis.
Institutional review board statement: DKT protocol has been issued by the regional regulatory agency NITp with n. PT.(02).06.580; local ethic committee Milano Area 3 has been notified of the study as an anonymous and non-interventional report.
Informed consent statement: Each patient signed informed consent at the time of listing for renal transplantation, and before renal transplantation itself. No further informed consent was required for this study because the analysis used anonymous clinical data that were collected after patients agreed to treatment by written consent.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
STROBE statement: The authors have read the STROBE statement-checklist of items, and the manuscript was prepared and revised according to the STROBE Statement-checklist of items.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Giacomo Colussi, MD, Chief Doctor, Division of Nephrology, Dialysis and Renal Transplantatation, ASST Grande Ospedale Territoriale Niguarda, Piazza Ospedale Maggiore, 3, Milan 20162,Italy. giacomo.colussi@ospedaleniguarda.it
Telephone: +39-2-64442521 Fax: +39-2-64442709
Received: February 28, 2018
Peer-review started: March 8, 2018
First decision: April 4, 2018
Revised: April 9, 2018
Accepted: May 11, 2018
Article in press: May 13, 2018
Published online: August 9, 2018
Processing time: 154 Days and 12.8 Hours
ARTICLE HIGHLIGHTS
Research background

In renal transplantation a hot topic is the best use of older donor grafts: these organs are associated with an higher risk of early and late graft failure, yet this donor category has become the most prevalent one in western countries. Pre-implantation biopsy of grafts from elderly donors is commonly used to guide in the acceptance/discard of organs, and/or in their allocation to single or dual kidney transplant.

Research motivation

There is no universal agreement in the literature on usefulness of biopsy to predict post-transplant graft outcome; additionally, a main concern with dual kidney allocation is a reduction of transplants made possible by available donors.

Research objectives

The main objectives of our study were to retrospectively compare outcome data of transplants with older donor grafts categorized according to pre-implantation histology into a low-score or high-score category; additionally, high-score grafts were compared by allocation to either dual kidney or single kidney transplant category.

Research methods

All renal-only transplants in our Center from 1 Jan 2000 to 30 Oct 2017 from donors older than 60 years and with available pre-implantation graft biopsy were retrospectively evaluated. Before Dec 2010 grafts were allocated only to single kidney transplant, irrespective of histology; after that date we adopted a biopsy-based protocol (DKT protocol), which dictated allocation to single kidney transplant of grafts with low histological score (1 to 4), and to dual kidney transplant of grafts with high histological score (4 to 7).

Research results

A total of 185 patients with pre-implantation biopsy were available, 102 with low histological score (4 or less), 83 with high histological score (5 to 8), of which 30 were allocated to single kidney transplant (score 5 to 8) and 53 to dual kidney transplant (score 5 to 7). Donors allocated to single kidney transplant did not differ between the low score and high score categories as concerns age, sex distribution, renal function, comorbidities, KDPI and KDRI indices, while they were older and with higher KDPI/KDRI indices in the dual kidney transplant category. Up to 10 years after transplant, we did not observe any differences in graft, patient and overall survival between recipients of a single kidney transplant with either low or high histological score, or between recipients of high histological score grafts allocated either to single or dual kidney transplant. These results were confirmed in a sub-analysis based only on the oldest donors (older than 70 years). We also calculated the total number of dialysis free life years in recipients of either a single or dual kidney transplant by available donors, showing a significantly higher value for recipients of a single kidney transplant up to the available follow-up of 6 years.

Research conclusions

Our study shows that the histological score in use in our transplant area does not predict post-transplant outcome in recipients of a single kidney transplant; additionally, allocation of grafts with similar histological score to single or dual kidney transplant is associated with equal survival up to the available follow-up of 6 years. We propose that renal biopsy is not indicated in older donors with preserved renal function and anatomy, and that organ allocation to single kidney transplant allows the best use of these donors. We propose that pre-implantation biopsy be limited to donors of any age with abnormal renal function, to ascertain type and reversibility of underlying pathology; dual kidney transplant allocation should be considered for bad function grafts with chronic histological pathology, provided that at least 50% viable tissue be reasonably ascertained.

Research perspectives

Main lesson of our study is that histological score scale in current clinical use does not allow to discriminate between organs which could or could not function adequately as single kidney transplant. This implies the risk of underutilization of available donors. A prospective randomization of equal score grafts to single or dual kidney transplant, and a longer follow-up are strongly desirable to ascertain any advantages or inconveniences of dual vs single kidney allocation.