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Vieira GM, Almeida TCS, Oliveira FP, Azzi PC, Rodrigues CF, Souza RL, Lacerda SMSN, Lages FS, Martins MD. Comparative Study of Acid Etching and SLA Surface Modification for Titanium Implants. MATERIALS (BASEL, SWITZERLAND) 2025; 18:1632. [PMID: 40271856 PMCID: PMC11990087 DOI: 10.3390/ma18071632] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/11/2025] [Revised: 02/10/2025] [Accepted: 02/12/2025] [Indexed: 04/25/2025]
Abstract
The dust generated during the sandblasting process of the sandblasted and acid-etched (SLA) method, commonly used to treat the surface of Ti dental implants, poses significant challenges in maintaining a clean manufacturing environment and ensuring safe working conditions. Nevertheless, surface modification remains crucial for improved performance of Ti dental implants. To address this problem and propose a clean and simple surface modification process to potentially replace SLA modification, this study aimed to characterize the surfaces of commercially pure Ti (cp-Ti) samples treated by acid etching and compare them with SLA-treated samples in terms of surface roughness (Rq), wettability (assessed through contact angle measurements), mineralized matrix deposition (evaluated through simulated body fluid [SBF] soaking), cell viability, cell differentiation (assessed based on alkaline phosphatase activity), and mineralization (assessed using MTT assay). Acid-etched surfaces exhibited nano- and micro-roughness and higher hydrophilicity than SLA surfaces, which is conducive to forming a highly bioactive TiO2 surface. Moreover, acid-etched samples exhibited earlier hydroxyapatite deposition after SBF soaking than SLA samples. Furthermore, the acid-etched surfaces were nontoxic and displayed significantly higher cell viability and differentiation after seven days than SLA surfaces. These findings suggest that acid etching is a viable alternative to the SLA method, likely offering superior surface bioactivity and biocompatibility.
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Affiliation(s)
- Gabriel M. Vieira
- Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte 31270-901, MG, Brazil; (T.C.S.A.); (F.P.O.); (P.C.A.); (C.F.R.)
| | - Tatiane C. S. Almeida
- Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte 31270-901, MG, Brazil; (T.C.S.A.); (F.P.O.); (P.C.A.); (C.F.R.)
| | - Fernanda P. Oliveira
- Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte 31270-901, MG, Brazil; (T.C.S.A.); (F.P.O.); (P.C.A.); (C.F.R.)
| | - Patrícia C. Azzi
- Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte 31270-901, MG, Brazil; (T.C.S.A.); (F.P.O.); (P.C.A.); (C.F.R.)
| | - Caio F. Rodrigues
- Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte 31270-901, MG, Brazil; (T.C.S.A.); (F.P.O.); (P.C.A.); (C.F.R.)
| | - Rafael L. Souza
- Instituto de Engenharia, Ciência e Tecnologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Janaúba 39440-000, MG, Brazil;
| | - Samyra Maria S. N. Lacerda
- Departamento de Odontologia Restauradora, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil;
| | - Frederico S. Lages
- Departamento de Morfologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil;
| | - Maximiliano D. Martins
- Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte 31270-901, MG, Brazil; (T.C.S.A.); (F.P.O.); (P.C.A.); (C.F.R.)
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Ma Z, Zhao Y, Xu Z, Zhang Y, Han Y, Jiang H, Sun P, Feng W. 3D-printed porous titanium rods equipped with vancomycin-loaded hydrogels and polycaprolactone membranes for intelligent antibacterial drug release. Sci Rep 2024; 14:21749. [PMID: 39294268 PMCID: PMC11411058 DOI: 10.1038/s41598-024-72457-1] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/01/2024] [Accepted: 09/06/2024] [Indexed: 09/20/2024] Open
Abstract
Implant-related infections pose significant challenges to orthopedic surgeries due to the high risk of severe complications. The widespread use of bioactive prostheses in joint replacements, featuring roughened surfaces and tight integration with the bone marrow cavity, has facilitated bacterial proliferation and complicated treatment. Developing antibacterial coatings for orthopedic implants has been a key research focus in recent years to address this critical issue. Researchers have designed coatings using various materials and antibacterial strategies. In this study, we fabricated 3D-printed porous titanium rods, incorporated vancomycin-loaded mPEG750-b-PCL2500 gel, and coated them with a PCL layer. We then evaluated the antibacterial efficacy through both in vitro and in vivo experiments. Our coating passively inhibits bacterial biofilm formation and actively controls antibiotic release in response to bacterial growth, providing a practical solution for proactive and sustained infection control. This study utilized 3D printing technology to produce porous titanium rod implants simulating bioactive joint prostheses. The porous structure of the titanium rods was used to load a thermoresponsive gel, mPEG750-b-PCL2500 (PEG: polyethylene glycol; PCL: polycaprolactone), serving as a novel drug delivery system carrying vancomycin for controlled antibiotic release. The assembly was then covered with a PCL membrane that inhibits bacterial biofilm formation early in infection and degrades when exposed to lipase solutions, mimicking enzymatic activity during bacterial infections. This setup provides infection-responsive protection and promotes drug release. We investigated the coating's controlled release, antibacterial capability, and biocompatibility through in vitro experiments. We established a Staphylococcus aureus infection model in rabbits, implanting titanium rods in the femoral medullary cavity. We evaluated the efficacy and safety of the composite coating in preventing implant-related infections using imaging, hematology, and pathology. In vitro experiments demonstrated that the PCL membrane stably protects encapsulated vancomycin during PBS immersion. The PCL membrane rapidly degraded at a lipase concentration of 0.2 mg/mL. The mPEG750-b-PCL2500 gel ensured stable and sustained vancomycin release, inhibiting bacterial growth. We investigated the antibacterial effect of the 3D-printed titanium material, coated with PCL and loaded with mPEG750-b-PCL2500 hydrogel, using a rabbit Staphylococcus aureus infection model. Imaging, hematology, and histopathology confirmed that our composite antibacterial coating exhibited excellent antibacterial effects and infection prevention, with good safety in trials. Our results indicate that the composite antibacterial coating effectively protects vancomycin in the hydrogel from premature release in the absence of bacterial infection. The outer PCL membrane inhibits bacterial growth and prevents biofilm formation. Upon contact with bacterial lipase, the PCL membrane rapidly degrades, releasing vancomycin for antibacterial action. The mPEG750-b-PCL2500 gel provides stable and sustained vancomycin release, prolonging its antibacterial effects. Our composite antibacterial coating demonstrates promising potential for clinical application.
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Affiliation(s)
- Zheru Ma
- Department of Bone and Joint Surgery, Orthopedic Center, The First Hospital of Jilin University, 72 Xinmin Street, Changchun, 130021, Jilin, China
| | - Yao Zhao
- Department of Bone and Joint Surgery, Orthopedic Center, The First Hospital of Jilin University, 72 Xinmin Street, Changchun, 130021, Jilin, China
| | - Zhe Xu
- Department of Bone and Joint Surgery, Orthopedic Center, The First Hospital of Jilin University, 72 Xinmin Street, Changchun, 130021, Jilin, China
- Weifang Traditional Chinese Hospital, Weifang, China
| | - Yao Zhang
- Department of Bone and Joint Surgery, Orthopedic Center, The First Hospital of Jilin University, 72 Xinmin Street, Changchun, 130021, Jilin, China
- Affiliated Hospital of Yangzhou University, Yangzhou, China
| | - Yu Han
- Department of Bone and Joint Surgery, Orthopedic Center, The First Hospital of Jilin University, 72 Xinmin Street, Changchun, 130021, Jilin, China
| | - Haozhuo Jiang
- Department of Bone and Joint Surgery, Orthopedic Center, The First Hospital of Jilin University, 72 Xinmin Street, Changchun, 130021, Jilin, China
| | - Peng Sun
- Department of Bone and Joint Surgery, Orthopedic Center, The First Hospital of Jilin University, 72 Xinmin Street, Changchun, 130021, Jilin, China
| | - Wei Feng
- Department of Bone and Joint Surgery, Orthopedic Center, The First Hospital of Jilin University, 72 Xinmin Street, Changchun, 130021, Jilin, China.
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Alshadidi AAF, Dommeti VK, Aldosari LIN, Hassan SAB, Okshah A, Merdji A, Roy S. Influence of surface texturing and coatings on mechanical properties and integration with bone tissue: an in silico study. Front Bioeng Biotechnol 2024; 12:1439262. [PMID: 39286343 PMCID: PMC11403329 DOI: 10.3389/fbioe.2024.1439262] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/27/2024] [Accepted: 08/05/2024] [Indexed: 09/19/2024] Open
Abstract
Introduction This investigation delves into the mechanical behaviour of titanium dental implants, a preferred choice for tooth replacement due to their superior reliability over alternative materials. The phenomenon of implant loosening, frequently induced by masticatory activities, underscores the significance of surface modification or texturing to bolster the interaction between the implant and bone tissue. This research comprehensively examines the effects of four distinct surface texturing techniques and five varied bone quality conditions on the biomechanical performance of these implants. Methods The scope of this study is delineated by its focus on implants of diameters 4 mm and 6 mm, with lengths measuring 9 mm and 12 mm respectively. Furthermore, the analysis incorporates the evaluation of four different coatings-hydroxyapatite, HA3TO, HA3Sr, and HA1.5TO1.5Sr-to investigate their efficacy in enhancing the osseointegration process on textured surfaces of dental implants. Results The experimental design entails the assessment of stress distribution within the implant and its coatings, alongside the strain exerted on the surrounding cancellous bone, under the conditions of an average vertical biting force. A comparative analysis between solid implants and those subjected to surface texturing techniques has been conducted. This comparison elucidates the advantageous microstrain profiles presented by certain textured surfaces, which are deemed more conducive to optimal osseointegration. Discussion Notably, across all examined textures, the application of hydroxyapatite (HA) and a modified HA composition (HA1.5TO1.5Sr) demonstrates significant improvements in mechanical stability, particularly in scenarios involving weak and very weak bone conditions. This study's findings contribute to the ongoing advancement in dental implant technology, emphasizing the critical role of surface texturing and coating strategies in promoting implant longevity and integration within the biomechanical environment of the human oral cavity.
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Affiliation(s)
- Abdulkhaliq Ali F Alshadidi
- Allied Dental Health Sciences Department, College of Medical Applied Sciences, King Khalid University, Abha, Saudi Arabia
| | - Vamsi Krishna Dommeti
- Department of Mechanical Engineering, SRM Institute of Science and Technology, Chennai, India
| | | | - Saeed Awod Bin Hassan
- Department of Restorative Dental Sciences "RDS" College of Dentistry, King Khalid University, Abha, Saudi Arabia
| | - Abdulmajeed Okshah
- Allied Dental Health Sciences Department, College of Medical Applied Sciences, King Khalid University, Abha, Saudi Arabia
| | - Ali Merdji
- Department of Mechanical Engineering, University of Mascara, Mascara, Algeria
| | - Sandipan Roy
- Department of Mechanical Engineering, SRM Institute of Science and Technology, Chennai, India
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Matsuura T, Komatsu K, Cheng J, Park G, Ogawa T. Beyond microroughness: novel approaches to navigate osteoblast activity on implant surfaces. Int J Implant Dent 2024; 10:35. [PMID: 38967690 PMCID: PMC11226592 DOI: 10.1186/s40729-024-00554-x] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/04/2024] [Accepted: 06/15/2024] [Indexed: 07/06/2024] Open
Abstract
Considering the biological activity of osteoblasts is crucial when devising new approaches to enhance the osseointegration of implant surfaces, as their behavior profoundly influences clinical outcomes. An established inverse correlation exists between osteoblast proliferation and their functional differentiation, which constrains the rapid generation of a significant amount of bone. Examining the surface morphology of implants reveals that roughened titanium surfaces facilitate rapid but thin bone formation, whereas smooth, machined surfaces promote greater volumes of bone formation albeit at a slower pace. Consequently, osteoblasts differentiate faster on roughened surfaces but at the expense of proliferation speed. Moreover, the attachment and initial spreading behavior of osteoblasts are notably compromised on microrough surfaces. This review delves into our current understanding and recent advances in nanonodular texturing, meso-scale texturing, and UV photofunctionalization as potential strategies to address the "biological dilemma" of osteoblast kinetics, aiming to improve the quality and quantity of osseointegration. We discuss how these topographical and physicochemical strategies effectively mitigate and even overcome the dichotomy of osteoblast behavior and the biological challenges posed by microrough surfaces. Indeed, surfaces modified with these strategies exhibit enhanced recruitment, attachment, spread, and proliferation of osteoblasts compared to smooth surfaces, while maintaining or amplifying the inherent advantage of cell differentiation. These technology platforms suggest promising avenues for the development of future implants.
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Affiliation(s)
- Takanori Matsuura
- Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, 10833 Le Conte Avenue B3-087, Box951668, Los Angeles, CA, 90095-1668, USA
| | - Keiji Komatsu
- Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, 10833 Le Conte Avenue B3-087, Box951668, Los Angeles, CA, 90095-1668, USA
| | - James Cheng
- Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, 10833 Le Conte Avenue B3-087, Box951668, Los Angeles, CA, 90095-1668, USA
- Division of Regenerative and Reconstructive Sciences, UCLA School of Dentistry, Los Angeles, USA
| | - Gunwoo Park
- Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, 10833 Le Conte Avenue B3-087, Box951668, Los Angeles, CA, 90095-1668, USA
| | - Takahiro Ogawa
- Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, 10833 Le Conte Avenue B3-087, Box951668, Los Angeles, CA, 90095-1668, USA.
- Division of Regenerative and Reconstructive Sciences, UCLA School of Dentistry, Los Angeles, USA.
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Emam SM, Moussa N. Signaling pathways of dental implants' osseointegration: a narrative review on two of the most relevant; NF-κB and Wnt pathways. BDJ Open 2024; 10:29. [PMID: 38580623 PMCID: PMC10997788 DOI: 10.1038/s41405-024-00211-w] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/25/2024] [Revised: 03/09/2024] [Accepted: 03/11/2024] [Indexed: 04/07/2024] Open
Abstract
INTRODUCTION Cell signaling pathways are the biological reactions that control cell functions and fate. They also directly affect the body reactions to implanted biomaterials. It is well-known that dental implants success depends on a successful integration with the alveolar bone: "osseointegration" which events comprise early and later responses to the implanted biomaterials. The early events are mainly immune-inflammatory responses to the implant considered by its microenvironment as a foreign body. Later reactions are osteogenic aiming to regulate bone formation and remodeling. All these events are controlled by the cell signaling pathways in an incredible harmonious coordination. AIM The number of pathways having a role in osseointegration is so big to be reviewed in a single article. So the aim of this review was to study only two of the most relevant ones: the inflammatory Nuclear Factor Kappa B (NF-κB) pathway regulating the early osseointegration events and the osteogenic Wnt pathway regulating later events. METHODS We conducted a literature review using key databases to provide an overview about the NF-κB and Wnt cell signaling pathways and their mutual relationship with dental implants. A simplified narrative approach was conducted to explain these cell signaling pathways, their mode of activation and how they are related to the cellular events of osseointegration. RESULTS AND CONCLUSION NF-κB and Wnt cell signaling pathways are important cross-talking pathways that are affected by the implant's material and surface characteristics. The presence of the implant itself in the bone alters the intracellular events of both pathways in the adjacent implant's cellular microenvironment. Both pathways have a great role in the success or failure of osseointegration. Such knowledge can offer a new hope to treat failed implants and enhance osseointegration in difficult cases. This is consistent with advances in Omics technologies that can change the paradigm of dental implant therapy.
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Affiliation(s)
- Samar Mohamed Emam
- Department of Prosthodontics, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
- Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.
| | - Nermine Moussa
- Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt
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Belloni A, Argentieri G, Orilisi G, Notarstefano V, Giorgini E, D'Addazio G, Orsini G, Caputi S, Sinjari B. New insights on collagen structural organization and spatial distribution around dental implants: a comparison between machined and laser-treated surfaces. J Transl Med 2024; 22:120. [PMID: 38297308 PMCID: PMC10829267 DOI: 10.1186/s12967-024-04906-4] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/23/2023] [Accepted: 01/18/2024] [Indexed: 02/02/2024] Open
Abstract
BACKGROUND One of the main factors for the osseointegration of dental implants is the development of an adequate soft tissue barrier, mainly composed by collagen, which protects the implant from bacterial development. The structural features of the peri-implant collagen are influenced by the implant components and, in particular, by the type of the surface. In the clinical practice, healing abutments are characterized by smooth surfaces, named machined. Recently, a new laser technique, Synthegra, has been developed to obtain a topography-controlled surface with micrometric regular pores that seems reducing the risk of peri-implantitis. Based on this background, this study aims investigating the structural organization and spatial distribution of collagen surrounding healing abutments characterized by laser-treated and machined surfaces. METHODS Gingiva portions surrounding custom-made healing abutments (HA), characterized by alternated laser-treated and machined surfaces, were collected and analyzed by combining Fourier Transform InfraRed Imaging (FTIRI) spectroscopy, a non-invasive and high-resolution bidimensional analytical technique, with histological and multivariate analyses. RESULTS Masson's trichrome staining, specific for collagen, highlighted a massive presence of collagen in all the analyzed samples, evidencing a surface-related spatial distribution. The nature of collagen, investigated by the FTIRI spectroscopy, appeared more abundant close to the laser-treated surface, with a perpendicular disposition of the bundles respect to the HA; conversely, a parallel distribution was observed around the machined surface. A different secondary structure was also found, with a higher amount of triple helices and a lower quantity of random coils in collagen close to the laser treated surfaces. CONCLUSIONS FTIRI spectroscopy demonstrates that the use of a laser treated transmucosal surface can improve the morphological organization of the peri-implant collagen, which presents a distribution more similar to that of natural teeth. TRIAL REGISTRATION This trial is registered with ClinicalTrials.gov Identifier: (Registration Number: NCT05754970). Registered 06/03/2023, retrospectively registered, https://clinicaltrials.gov/show/NCT05754970 .
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Affiliation(s)
- Alessia Belloni
- Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy
| | - Giulio Argentieri
- Electron Microscopy Laboratory, Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy
| | - Giulia Orilisi
- Department of Clinical Sciences and Stomatology, Polytechnic University of Marche, Ancona, Italy
| | - Valentina Notarstefano
- Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy
| | - Elisabetta Giorgini
- Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy
| | - Gianmaria D'Addazio
- Electron Microscopy Laboratory, Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy
| | - Giovanna Orsini
- Department of Clinical Sciences and Stomatology, Polytechnic University of Marche, Ancona, Italy.
| | - Sergio Caputi
- Electron Microscopy Laboratory, Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy
| | - Bruna Sinjari
- Electron Microscopy Laboratory, Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy
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Liang Y, Leng Y, Zhang J. Influence of clinical zirconia surface treatments on microscopic characteristics and adhesion-proliferation behavior of human gingival fibroblasts. JOURNAL OF STOMATOLOGY, ORAL AND MAXILLOFACIAL SURGERY 2023; 124:101564. [PMID: 37453567 DOI: 10.1016/j.jormas.2023.101564] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/09/2023] [Revised: 07/10/2023] [Accepted: 07/12/2023] [Indexed: 07/18/2023]
Abstract
Zirconia is favored in dental implant applications due to its biocompatibility, mechanical properties, and esthetic appeal, particularly in its interaction with soft oral tissues such as the gingiva. To optimize zirconia for clinical use, surface treatments like sanding and polishing are essential. The aim of this study was to investigate the effects of clinical surface treatments on the microscopic characteristics of zirconia and the adhesion and proliferation of human gingival fibroblasts (HGFs). Scanning electron microscopy (SEM) and fluorescence microscopy were utilized to examine the microscopic morphology and roughness resulting from various clinical surface treatment procedures on zirconia and to assess their impact on the microscopic appearance and behavior of HGFs. The results showed that the application of surface treatment procedures, particularly polishing treatments, resulted in the formation of a regular shallow groove morphology and a significant reduction in roughness in zirconia. This was accompanied by improved cell proliferation, cell adhesion, and the expression of integrin β1 in HGFs. The results suggest that smoother zirconia surfaces promote better cell-material interactions, potentially improving the clinical success of dental implants. This research contributes to our understanding of the optimal surface roughness for soft tissue adhesion and the effect of different micro-morphologies on HGF attachment.
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Affiliation(s)
- Yajing Liang
- School of Stomatology, Capital Medical University, Beijing, PR China
| | - Yanjun Leng
- School of Stomatology, Central South University, Changsha, PR China
| | - Jiebing Zhang
- School of Stomatology, Capital Medical University, Beijing, PR China.
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Martins de Sousa K, Linklater DP, Murdoch BJ, Al Kobaisi M, Crawford RJ, Judge R, Dashper S, Sloan AJ, Losic D, Ivanova EP. Modulation of MG-63 Osteogenic Response on Mechano-Bactericidal Micronanostructured Titanium Surfaces. ACS APPLIED BIO MATERIALS 2023; 6:1054-1070. [PMID: 36880728 DOI: 10.1021/acsabm.2c00952] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 03/08/2023]
Abstract
Despite recent advances in the development of orthopedic devices, implant-related failures that occur as a result of poor osseointegration and nosocomial infection are frequent. In this study, we developed a multiscale titanium (Ti) surface topography that promotes both osteogenic and mechano-bactericidal activity using a simple two-step fabrication approach. The response of MG-63 osteoblast-like cells and antibacterial activity toward Pseudomonas aeruginosa and Staphylococcus aureus bacteria was compared for two distinct micronanoarchitectures of differing surface roughness created by acid etching, using either hydrochloric acid (HCl) or sulfuric acid (H2SO4), followed by hydrothermal treatment, henceforth referred to as either MN-HCl or MN-H2SO4. The MN-HCl surfaces were characterized by an average surface microroughness (Sa) of 0.8 ± 0.1 μm covered by blade-like nanosheets of 10 ± 2.1 nm thickness, whereas the MN-H2SO4 surfaces exhibited a greater Sa value of 5.8 ± 0.6 μm, with a network of nanosheets of 20 ± 2.6 nm thickness. Both micronanostructured surfaces promoted enhanced MG-63 attachment and differentiation; however, cell proliferation was only significantly increased on MN-HCl surfaces. In addition, the MN-HCl surface exhibited increased levels of bactericidal activity, with only 0.6% of the P. aeruginosa cells and approximately 5% S. aureus cells remaining viable after 24 h when compared to control surfaces. Thus, we propose the modulation of surface roughness and architecture on the micro- and nanoscale to achieve efficient manipulation of osteogenic cell response combined with mechanical antibacterial activity. The outcomes of this study provide significant insight into the further development of advanced multifunctional orthopedic implant surfaces.
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Affiliation(s)
| | - Denver P Linklater
- School of Science, STEM College, RMIT University, Melbourne, Victoria 3000, Australia
- Department of Biomedical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia
| | - Billy J Murdoch
- RMIT Microscopy and Microanalysis Facility, STEM College, RMIT University, Melbourne, Victoria 3000, Australia
| | - Mohammad Al Kobaisi
- School of Engineering, STEM College, RMIT University, Melbourne, Victoria 3000, Australia
| | - Russell J Crawford
- School of Science, STEM College, RMIT University, Melbourne, Victoria 3000, Australia
| | - Roy Judge
- Melbourne Dental School, Faculty of Medicine, Dentistry & Health Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia
| | - Stuart Dashper
- Melbourne Dental School, Faculty of Medicine, Dentistry & Health Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia
| | - Alastair J Sloan
- Melbourne Dental School, Faculty of Medicine, Dentistry & Health Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia
| | - Dusan Losic
- School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, South Australia 5005, Australia
| | - Elena P Ivanova
- School of Science, STEM College, RMIT University, Melbourne, Victoria 3000, Australia
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9
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Liao TY, King PC, Zhu D, Crawford RJ, Ivanova EP, Thissen H, Kingshott P. Surface Characteristics and Bone Biocompatibility of Cold-Sprayed Porous Titanium on Polydimethylsiloxane Substrates. ACS Biomater Sci Eng 2023; 9:1402-1421. [PMID: 36813258 DOI: 10.1021/acsbiomaterials.2c01506] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/24/2023]
Abstract
A variant of the cold spray (CS) technique was applied for the functionalization of polymer-based materials such as polydimethylsiloxane (PDMS) to improve the extent of mammalian cell interactions with these substrates. This was demonstrated by the embedment of porous titanium (pTi) into PDMS substrates using a single-step CS technique. CS processing parameters such as gas pressure and temperature were optimized to achieve the mechanical interlocking of pTi in the compressed PDMS to fabricate a unique hierarchical morphology possessing micro-roughness. As evidenced by the preserved porous structure, the pTi particles did not undergo any significant plastic deformation upon impact with the polymer substrate. The thickness of the particle embedment layer was determined, by cross-sectional analysis, ranging from 120 μm to over 200 μm. The behavior of osteoblast-like cells MG63 coming into contact with the pTi-embedded PDMS was examined. The results showed that the pTi-embedded PDMS samples promoted 80-96% of cell adhesion and proliferation during the early stages of incubation. The low cytotoxicity of the pTi-embedded PDMS was confirmed, with cell viability of the MG63 cells being above 90%. Furthermore, the pTi-embedded PDMS facilitated the production of alkaline phosphatase and calcium deposition in the MG63 cells, as demonstrated by the higher amount of alkaline phosphatase (2.6 times) and calcium (10.6 times) on the pTi-embedded PDMS sample fabricated at 250 °C, 3 MPa. Overall, the work demonstrated that the CS process provided flexibility in the parameters used for the production of the modified PDMS substrates and is highly efficient for the fabrication of coated polymer products. The results obtained in this study suggest that a tailorable porous and rough architecture could be achieved that promoted osteoblast function, indicating that the method has promise in the design of titanium-polymer composite materials applied to biomaterials used in musculoskeletal applications.
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Affiliation(s)
- Tzu-Ying Liao
- School of Science, Computing & Engineering Technologies, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
- Australian Research Council (ARC) Training Centre in Surface Engineering for Advanced Materials (SEAM), Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
- CSIRO Manufacturing, Clayton, Victoria 3168, Australia
| | - Peter C King
- Australian Research Council (ARC) Training Centre in Surface Engineering for Advanced Materials (SEAM), Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
- CSIRO Manufacturing, Clayton, Victoria 3168, Australia
| | - Deming Zhu
- School of Science, Computing & Engineering Technologies, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
| | - Russell J Crawford
- Australian Research Council (ARC) Training Centre in Surface Engineering for Advanced Materials (SEAM), Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
- College of STEM, School of Science, RMIT University, Melbourne, Victoria 3000, Australia
| | - Elena P Ivanova
- Australian Research Council (ARC) Training Centre in Surface Engineering for Advanced Materials (SEAM), Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
- College of STEM, School of Science, RMIT University, Melbourne, Victoria 3000, Australia
| | - Helmut Thissen
- Australian Research Council (ARC) Training Centre in Surface Engineering for Advanced Materials (SEAM), Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
- CSIRO Manufacturing, Clayton, Victoria 3168, Australia
| | - Peter Kingshott
- School of Science, Computing & Engineering Technologies, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
- Australian Research Council (ARC) Training Centre in Surface Engineering for Advanced Materials (SEAM), Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
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10
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Dias Corpa Tardelli J, Duarte Firmino AC, Ferreira I, Cândido dos Reis A. Influence of the roughness of dental implants obtained by additive manufacturing on osteoblastic adhesion and proliferation: A systematic review. Heliyon 2022; 8:e12505. [PMID: 36643331 PMCID: PMC9834751 DOI: 10.1016/j.heliyon.2022.e12505] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/20/2022] [Revised: 10/29/2022] [Accepted: 12/13/2022] [Indexed: 12/26/2022] Open
Abstract
Objective Critically analyzed the existing literature to answer the question "What is the influence of roughness of surfaces for dental implants obtained by additive manufacturing compared to machined on osteoblastic cell adhesion and proliferation?" Design This systematic review followed the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) and was registered in the Open Science Framework. The personalized search strategy was applied to Embase, Pub Med, Scopus, and Science Direct databases and Google Scholar and ProQuest grey literature. The selection process was carried out in two stages independently by two reviewers according to the eligibility criteria. The risk of bias was analyzed using a checklist of important parameters to be considered. Results When applying the search strategy on databases 223 articles were found, after removing the duplicates, 171 were analyzed by title and abstract of which 25 were selected for full reading, of these, 6 met the eligibility criteria. 2 studies were included from the reference list totaling 8 articles included in this systematic review and none were included from the Grey Literature. 7 had a low risk of bias and 1 moderate. Conclusions 1) Roughness is a property that must be analyzed and correlated with the chemical composition, intrinsic to the alloy and resulting from the surface treatment; morphology of topographic peaks and valleys; printing technique and its parameters; 2) Need for more studies on the biomolecular level to elucidate the mechanism by which the roughness and the morphology of topographical peaks and valleys descriptive of roughness influence osteoblastic adhesion and proliferation.
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11
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Bayrak M, Kocak-Oztug NA, Gulati K, Cintan S, Cifcibasi E. Influence of Clinical Decontamination Techniques on the Surface Characteristics of SLA Titanium Implant. NANOMATERIALS (BASEL, SWITZERLAND) 2022; 12:4481. [PMID: 36558334 PMCID: PMC9784882 DOI: 10.3390/nano12244481] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 10/24/2022] [Revised: 12/04/2022] [Accepted: 12/12/2022] [Indexed: 06/17/2023]
Abstract
The study aims: 1. To perform diode laser, titanium (Ti) brush, and Ti curette treatment on sandblasted and acid-etched (SLA) Ti surfaces, with/without H2O2 and CHX, 2. To investigate the influence of decontamination techniques on implant surface topography and hydrophilicity. Diode laser, Ti brush, and Ti curette treatments were performed on the Grade 4 Ti discs, with/without treatment with 3% H2O2 solution or 0.2% CHX. Surface characteristics were investigated via SEM, optical profilometry, and water contact angle meter. SEM findings revealed flat and scratched areas when treated with Ti curette and Ti brush. For diode laser, SEM showed melting in specific areas. Ra and Rt values were lower in all test groups than in the control group (p < 0.05). The adjunctive chemical treatment showed negligible effects in SEM images and surface roughness measurements compared to laser and mechanical treatment-only groups. H2O2 treatment resulted in enhanced hydrophilicity in either treatment modalities with a significant difference compared to the negative control group (p < 0.05). In all test groups, the hydrophilicity was enhanced compared to the negative control group (p < 0.05). Diode laser treatment had the least disruptive effect on the Ti surface characteristics. The use of other mechanical methods caused significant alterations in the surface roughness.
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Affiliation(s)
- Meltem Bayrak
- Faculty of Dentistry, Department of Periodontology, Istanbul University, Istanbul 34116, Turkey
- Institute of Graduate Studies in Health Sciences, Department of Periodontology, Istanbul University, Istanbul 34126, Turkey
| | - Necla Asli Kocak-Oztug
- Faculty of Dentistry, Department of Periodontology, Istanbul University, Istanbul 34116, Turkey
- School of Dentistry, The University of Queensland, Herston, QLD 4006, Australia
| | - Karan Gulati
- School of Dentistry, The University of Queensland, Herston, QLD 4006, Australia
| | - Serdar Cintan
- Faculty of Dentistry, Department of Periodontology, Istanbul University, Istanbul 34116, Turkey
| | - Emine Cifcibasi
- Faculty of Dentistry, Department of Periodontology, Istanbul University, Istanbul 34116, Turkey
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12
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Roy M, Corti A, Dorocka-Bobkowska B, Pompella A. Positive Effects of UV-Photofunctionalization of Titanium Oxide Surfaces on the Survival and Differentiation of Osteogenic Precursor Cells-An In Vitro Study. J Funct Biomater 2022; 13:jfb13040265. [PMID: 36547525 PMCID: PMC9783962 DOI: 10.3390/jfb13040265] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/28/2022] [Revised: 11/20/2022] [Accepted: 11/22/2022] [Indexed: 11/30/2022] Open
Abstract
INTRODUCTION The UVC-irradiation ("UV-photofunctionalization") of titanium dental implants has proved to be capable of removing carbon contamination and restoring the ability of titanium surfaces to attract cells involved in the process of osteointegration, thus significantly enhancing the biocompatibility of implants and favoring the post-operative healing process. To what extent the effect of UVC irradiation is dependent on the type or the topography of titanium used, is still not sufficiently established. OBJECTIVE The present study was aimed at analyzing the effects of UV-photofunctionalization on the TiO2 topography, as well as on the gene expression patterns and the biological activity of osteogenic cells, i.e., osteogenic precursors cultured in vitro in the presence of different titanium specimens. METHODOLOGY The analysis of the surface roughness was performed by atomic force microscopy (AFM) on machined surface grade 2, and sand-blasted/acid-etched surface grades 2 and 4 titanium specimens. The expression of the genes related with the process of healing and osteogenesis was studied in the MC3T3-E1 pre-osteoblastic murine cells, as well as in MSC murine stem cells, before and after exposure to differently treated TiO2 surfaces. RESULTS The AFM determinations showed that the surface topographies of titanium after the sand-blasting and acid-etching procedures, look very similar, independently of the grade of titanium. The UVC-irradiation of the TiO2 surface was found to induce an increase in the cell survival, attachment and proliferation, which was positively correlated with an increased expression of the osteogenesis-related genes Runx2 and alkaline phosphatase (ALP). CONCLUSION Overall, our findings expand and further support the current view that UV-photofunctionalization can indeed restore biocompatibility and osteointegration of TiO2 implants, and suggest that this at least in part occurs through a stimulation of the osteogenic differentiation of the precursor cells.
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Affiliation(s)
- Marco Roy
- Department of Prosthodontics and Gerostomatology, Poznan University of Medical Sciences, 60-792 Poznan, Poland
- Correspondence:
| | - Alessandro Corti
- Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa Medical School, 56126 Pisa, Italy
| | - Barbara Dorocka-Bobkowska
- Department of Prosthodontics and Gerostomatology, Poznan University of Medical Sciences, 60-792 Poznan, Poland
| | - Alfonso Pompella
- Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa Medical School, 56126 Pisa, Italy
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13
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Effects of Different Titanium Surface Treatments on Adhesion, Proliferation and Differentiation of Bone Cells: An In Vitro Study. J Funct Biomater 2022; 13:jfb13030143. [PMID: 36135578 PMCID: PMC9503392 DOI: 10.3390/jfb13030143] [Citation(s) in RCA: 16] [Impact Index Per Article: 5.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/08/2022] [Revised: 08/31/2022] [Accepted: 09/01/2022] [Indexed: 11/17/2022] Open
Abstract
The objective of this study was to evaluate the impacts of different sandblasting procedures in acid etching of Ti6Al4V surfaces on osteoblast cell behavior, regarding various physicochemical and topographical parameters. Furthermore, differences in osteoblast cell behavior between cpTi and Ti6Al4V SA surfaces were evaluated. Sandblasting and subsequent acid etching of cpTi and Ti6Al4V discs was performed with Al2O3 grains of different sizes and with varying blasting pressures. The micro- and nano-roughness of the experimental SA surfaces were analyzed via confocal, atomic force and scanning electron microscopy. Surface free energy and friction coefficients were determined. hFOB 1.19 cells were seeded to evaluate adhesion, proliferation and osteoblastic differentiation for up to 12 d via crystal violet assays, MTT assays, ALP activity assays and Alizarin Red staining assays. Differences in blasting procedures had significant impacts on surface macro- and micro-topography. The crystal violet assay revealed a significant inverse relationship between blasting grain size and hFOB cell growth after 7 days. This trend was also visible in the Alizarin Red assays staining after 12 d: there was significantly higher biomineralization visible in the group that was sandblasted with smaller grains (F180) when compared to standard-grain-size groups (F70). SA samples treated with reduced blasting pressure exhibited lower hFOB adhesion and growth capabilities at initial (2 h) and later time points for up to 7 days, when compared to the standard SA surface, even though micro-roughness and other relevant surface parameters were similar. Overall, etched-only surfaces consistently exhibited equivalent or higher adhesion, proliferation and differentiation capabilities when compared to all other sandblasted and etched surfaces. No differences were found between cpTi and Ti6Al4V SA surfaces. Subtle modifications in the blasting protocol for Ti6Al4V SA surfaces significantly affect the proliferative and differentiation behavior of human osteoblasts. Surface roughness parameters are not sufficient to predict osteoblast behavior on etched Ti6Al4V surfaces.
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14
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Han J, Li Z, Sun Y, Cheng F, Zhu L, Zhang Y, Zhang Z, Wu J, Wang J. Surface Roughness and Biocompatibility of Polycaprolactone Bone Scaffolds: An Energy-Density-Guided Parameter Optimization for Selective Laser Sintering. Front Bioeng Biotechnol 2022; 10:888267. [PMID: 35898639 PMCID: PMC9309791 DOI: 10.3389/fbioe.2022.888267] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/02/2022] [Accepted: 05/23/2022] [Indexed: 11/13/2022] Open
Abstract
Three-dimensional porous polycaprolactone (PCL) bone scaffolds prepared by selective laser sintering (SLS) have demonstrated great potential in the repair of non-load-bearing bone defects. The microgeometry and surface roughness of PCL scaffolds during the SLS process may change the biocompatibility and bioactivity of the scaffolds. However, in addition to the widely concerned mechanical properties and structural accuracy of scaffolds, there is still a lack of systematic research on how SLS process parameters affect the surface roughness of PCL scaffolds and the relationship between roughness and biocompatibility of scaffolds. In this study, we use the energy density model (EDM) combined with the thermodynamic properties of PCL powder to calculate the energy density range (Ed1–Ed3) suitable for PCL sintering. Five PCL scaffolds with different laser powers and scanning speeds were prepared; their dimensional accuracy, mechanical strength, and surface properties were comprehensively evaluated, and the bioactivities were compared through the attachment and proliferation of MC3T3-E1 cells on the scaffolds. It was found that the high energy density (Ed3) reduced the shape fidelity related to pore size and porosity, and the dense and smooth surface of the scaffolds showed poor cytocompatibility, while the low energy density (Ed1) resulted in weak mechanical properties, but the rough surface caused by incomplete sintered PCL particles facilitated the cell adhesion and proliferation. Therefore, the surface roughness and related biocompatibility of PCL bone scaffolds should be considered in energy-density-guided SLS parameter optimization.
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Affiliation(s)
- Jian Han
- High Magnetic Field Laboratory, CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China
- University of Science and Technology of China, Hefei, China
| | - Zehua Li
- High Magnetic Field Laboratory, CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China
- University of Science and Technology of China, Hefei, China
| | - Yuxuan Sun
- University of Science and Technology of China, Hefei, China
| | - Fajun Cheng
- High Magnetic Field Laboratory, CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
| | - Lei Zhu
- High Magnetic Field Laboratory, CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China
| | - Yaoyao Zhang
- High Magnetic Field Laboratory, CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China
- University of Science and Technology of China, Hefei, China
| | - Zirui Zhang
- School of Electronic Engineering and Intelligent Manufacturing, Anqing Normal University, Anqing, China
| | - Jinzhe Wu
- School of Electronic Engineering, Naval University of Engineering, Wuhan, China
- *Correspondence: Jinzhe Wu, ; Junfeng Wang,
| | - Junfeng Wang
- High Magnetic Field Laboratory, CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China
- University of Science and Technology of China, Hefei, China
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- Institutes of Physical Science and Information Technology, Anhui University, Hefei, China
- *Correspondence: Jinzhe Wu, ; Junfeng Wang,
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15
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López-Valverde N, López-Valverde A, Cortés MP, Rodríguez C, Macedo De Sousa B, Aragoneses JM. Bone Quantification Around Chitosan-Coated Titanium Dental Implants: A Preliminary Study by Micro-CT Analysis in Jaw of a Canine Model. Front Bioeng Biotechnol 2022; 10:858786. [PMID: 35464727 PMCID: PMC9023049 DOI: 10.3389/fbioe.2022.858786] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/20/2022] [Accepted: 03/17/2022] [Indexed: 12/11/2022] Open
Abstract
Surface treatments of Ti in the dental implant industry are performed with the aim of in-creasing its bioactivity and osseointegration capacity. Chitosan (Cht) is a polysaccharide that has been proposed as a promising biomaterial in tissue engineering and bone regeneration, due to its ability to stimulate the recruitment and adhesion of osteogenic progenitor cells. The aim of our preliminary study was to evaluate, by micro-computed tomography (micro-CT), the osseointegration and bone formation around Cht-coated implants and to compare them with conventional surface-etched implants (SLA type). Four im-plants (8.5 mm length × 3.5 mm Ø) per hemiarch, were inserted into the jaws of five dogs, divided into two groups: chitosan-coated implant group (ChtG) and control group (CG). Twelve weeks after surgery, euthanasia was performed, and sectioned bone blocks were obtained and scanned by micro-CT and two bone parameters were measured: bone in contact with the implant surface (BCIS) and peri-implant bone area (PIBA). For BCIS and PIBA statistically significant values were obtained for the ChtG group with respect to CG (p = 0.005; p = 0.014 and p < 0.001 and p = 0.002, respectively). The results, despite the limitations, demonstrated the usefulness of chitosan coatings. However, studies with larger sample sizes and adequate experimental models would be necessary to confirm the results.
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Affiliation(s)
- Nansi López-Valverde
- Department of Medicine and Medical Specialties, Faculty of Health Sciences, Universidad Alcalá de Henares, Alcalá de Henares, Spain
| | - Antonio López-Valverde
- Department of Surgery, University of Salamanca, Instituto de Investigación Biomédica de Sala-manca (IBSAL), Salamanca, Spain
- *Correspondence: Antonio López-Valverde,
| | - Marta Paz Cortés
- Faculty of Dentistry, Universidad Alfonso X El Sabio, Villanueva de la Cañada, Spain
| | - Cinthia Rodríguez
- Department of Dentistry, Universidad Federico Henríquez y Carvajal, Santo Domingo, Dominican Republic
| | - Bruno Macedo De Sousa
- Institute for Occlusion and Orofacial Pain Faculty of Medicine, University of Coimbra, Polo I‐Edifício Central Rua Larga, Coimbra, Portugal
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16
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Immunomodulatory Properties and Osteogenic Activity of Polyetheretherketone Coated with Titanate Nanonetwork Structures. Int J Mol Sci 2022; 23:ijms23020612. [PMID: 35054795 PMCID: PMC8775651 DOI: 10.3390/ijms23020612] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/15/2021] [Revised: 12/31/2021] [Accepted: 01/01/2022] [Indexed: 12/23/2022] Open
Abstract
Polyetheretherketone (PEEK) is a potential substitute for conventional metallic biomedical implants owing to its superior mechanical and chemical properties, as well as biocompatibility. However, its inherent bio-inertness and poor osseointegration limit its use in clinical applications. Herein, thin titanium films were deposited on the PEEK substrate by plasma sputtering, and porous nanonetwork structures were incorporated on the PEEK surface by alkali treatment (PEEK-TNS). Changes in the physical and chemical characteristics of the PEEK surface were analyzed to establish the interactions with cell behaviors. The osteoimmunomodulatory properties were evaluated using macrophage cells and osteoblast lineage cells. The functionalized nanostructured surface of PEEK-TNS effectively promoted initial cell adhesion and proliferation, suppressed inflammatory responses, and induced macrophages to anti-inflammatory M2 polarization. Compared with PEEK, PEEK-TNS provided a more beneficial osteoimmune environment, including increased levels of osteogenic, angiogenic, and fibrogenic gene expression, and balanced osteoclast activities. Furthermore, the crosstalk between macrophages and osteoblast cells showed that PEEK-TNS could provide favorable osteoimmunodulatory environment for bone regeneration. PEEK-TNS exhibited high osteogenic activity, as indicated by alkaline phosphatase activity, osteogenic factor production, and the osteogenesis/osteoclastogenesis-related gene expression of osteoblasts. The study establishes that the fabrication of titanate nanonetwork structures on PEEK surfaces could extract an adequate immune response and favorable osteogenesis for functional bone regeneration. Furthermore, it indicates the potential of PEEK-TNS in implant applications.
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17
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Stoetzel S, Malhan D, Wild U, Helbing C, Hassan F, Attia S, Jandt KD, Heiss C, El Khassawna T. Osteocytes Influence on Bone Matrix Integrity Affects Biomechanical Competence at Bone-Implant Interface of Bioactive-Coated Titanium Implants in Rat Tibiae. Int J Mol Sci 2021; 23:374. [PMID: 35008800 PMCID: PMC8745552 DOI: 10.3390/ijms23010374] [Citation(s) in RCA: 11] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/04/2021] [Revised: 12/23/2021] [Accepted: 12/28/2021] [Indexed: 02/07/2023] Open
Abstract
Osseointegration is a prerequisite for the long-term success of implants. Titanium implants are preferred for their biocompatibility and mechanical properties. Nonetheless, the need for early and immediate loading requires enhancing these properties by adding bioactive coatings. In this preclinical study, extracellular matrix properties and cellular balance at the implant/bone interface was examined. Polyelectrolyte multilayers of chitosan and gelatin or with chitosan and Hyaluronic acid fabricated on titanium alloy using a layer-by-layer self-assembly process were compared with native titanium alloy. The study aimed to histologically evaluate bone parameters that correlate to the biomechanical anchorage enhancement resulted from bioactive coatings of titanium implants in a rat animal model. Superior collagen fiber arrangements and an increased number of active osteocytes reflected a significant improvement of bone matrix quality at the bone interface of the chitosan/gelatin-coated titan implants over chitosan/hyaluronic acid-coated and native implants. Furthermore, the numbers and localization of osteoblasts and osteoclasts in the reparative and remodeling phases suggested a better cellular balance in the chitosan/Gel-coated group over the other two groups. Investigating the micro-mechanical properties of bone tissue at the interface can elucidate detailed discrepancies between different promising bioactive coatings of titanium alloys to maximize their benefit in future medical applications.
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Affiliation(s)
- Sabine Stoetzel
- Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig-University, Aulweg 128, 35392 Giessen, Germany; (S.S.); (D.M.); (U.W.); (F.H.); (C.H.)
| | - Deeksha Malhan
- Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig-University, Aulweg 128, 35392 Giessen, Germany; (S.S.); (D.M.); (U.W.); (F.H.); (C.H.)
| | - Ute Wild
- Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig-University, Aulweg 128, 35392 Giessen, Germany; (S.S.); (D.M.); (U.W.); (F.H.); (C.H.)
| | - Christian Helbing
- Chair of Materials Science (CMS), Otto Schott Institute of Materials Research (OSIM), Faculty of Physics and Astronomy, Friedrich Schiller University Jena, Löbdergraben 32, 07743 Jena, Germany; (C.H.); (K.D.J.)
| | - Fathi Hassan
- Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig-University, Aulweg 128, 35392 Giessen, Germany; (S.S.); (D.M.); (U.W.); (F.H.); (C.H.)
| | - Sameh Attia
- Department of Oral and Maxillofacial Surgery, Justus-Liebig University of Giessen, Klinikstrasse 33, 35392 Giessen, Germany;
| | - Klaus D. Jandt
- Chair of Materials Science (CMS), Otto Schott Institute of Materials Research (OSIM), Faculty of Physics and Astronomy, Friedrich Schiller University Jena, Löbdergraben 32, 07743 Jena, Germany; (C.H.); (K.D.J.)
| | - Christian Heiss
- Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig-University, Aulweg 128, 35392 Giessen, Germany; (S.S.); (D.M.); (U.W.); (F.H.); (C.H.)
- Department of Trauma, Hand and Reconstructive Surgery, Justus-Liebig University Giessen, Rudolf-Buchheim-Strasse 7, 35392 Giessen, Germany
| | - Thaqif El Khassawna
- Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig-University, Aulweg 128, 35392 Giessen, Germany; (S.S.); (D.M.); (U.W.); (F.H.); (C.H.)
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18
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Wang L, Zheng F, Song R, Zhuang L, Yang M, Suo J, Li L. Integrins in the Regulation of Mesenchymal Stem Cell Differentiation by Mechanical Signals. Stem Cell Rev Rep 2021; 18:126-141. [PMID: 34536203 DOI: 10.1007/s12015-021-10260-5] [Citation(s) in RCA: 22] [Impact Index Per Article: 5.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 09/02/2021] [Indexed: 10/20/2022]
Abstract
Mesenchymal stem cells (MSCs) can sense and convert mechanical stimuli signals into a chemical response. Integrins are involved in the mechanotransduction from inside to outside and from outside to inside, and ultimately affect the fate of MSCs responding to different mechanical signals. Different integrins participate in different signaling pathways to regulate MSCs multi-differentiation. In this review, we summarize the latest advances in the effects of mechanical signals on the differentiation of MSCs, the importance of integrins in mechanotransduction, the relationship between integrin heterodimers and different mechanical signals, and the interaction among mechanical signals. We put forward our views on the prospect and challenges of developing mechanical biology in tissue engineering and regenerative medicine.
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Affiliation(s)
- Lei Wang
- Department of Gastrointestinal Surgery, Jilin University First Hospital, Jilin University, 130021, Changchun, People's Republic of China
| | - Fuwen Zheng
- Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China
| | - Ruixue Song
- Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China
| | - Lequan Zhuang
- Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China
| | - Ming Yang
- Department of Molecular Biology, College of Basic Medical Sciences, Jilin University, 130021, Changchun, People's Republic of China.
| | - Jian Suo
- Department of Gastrointestinal Surgery, Jilin University First Hospital, Jilin University, 130021, Changchun, People's Republic of China.
| | - Lisha Li
- The Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, 130021, Changchun, People's Republic of China.
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19
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Padilha Fontoura C, Ló Bertele P, Machado Rodrigues M, Elisa Dotta Maddalozzo A, Frassini R, Silvestrin Celi Garcia C, Tomaz Martins S, Crespo JDS, Figueroa CA, Roesch-Ely M, Aguzzoli C. Comparative Study of Physicochemical Properties and Biocompatibility (L929 and MG63 Cells) of TiN Coatings Obtained by Plasma Nitriding and Thin Film Deposition. ACS Biomater Sci Eng 2021; 7:3683-3695. [PMID: 34291900 DOI: 10.1021/acsbiomaterials.1c00393] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Abstract
Ti6Al4V is one of the most lightweight, mechanically resistant, and appropriate for biologically induced corrosion alloys. However, surface properties often must be tuned for fitting into biomedical applications, and therefore, surface modification is of paramount importance to carry on its use. This work compares the interaction between two different cell lines (L929 fibroblasts and osteoblast-like MG63) and medical grade Ti6Al4V after surface modification by plasma nitriding or thin film deposition. We studied the adhesion of these two cell lines, exploring which trends are consistent for cell behavior, correlating with osseointegration and in vivo conditions. Modified surfaces were analyzed through several physicochemical characterization techniques. Plasma nitriding led to a more pronounced increase in surface roughness, a thicker aluminum-free layer, made up of diverse titanium nitride phases, whereas thin film deposition resulted in a single-phase pure titanium nitride layer that leveled the ridged topography. The selective adhesion of osteoblast-like cells over fibroblasts was observed in nitrided samples but not in thin film deposited films, indicating that the competitive cellular behavior is more pronounced in plasma nitrided surfaces. The obtained coatings presented an appropriate performance for its use in biomedical-aimed applications, including the possibility of a higher success rate in osseointegration of implants.
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Affiliation(s)
- Cristian Padilha Fontoura
- Graduate Program in Materials Science and Engineering (PPGMAT), University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560 Brazil
| | - Patrícia Ló Bertele
- Graduate Program in Materials Science and Engineering (PPGMAT), University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560 Brazil
| | - Melissa Machado Rodrigues
- Graduate Program in Materials Science and Engineering (PPGMAT), University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560 Brazil
| | - Ana Elisa Dotta Maddalozzo
- Graduate Program in Materials Science and Engineering (PPGMAT), University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560 Brazil
- Institute of Biotechnology, University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560, Brazil
| | - Rafaele Frassini
- Institute of Biotechnology, University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560, Brazil
| | - Charlene Silvestrin Celi Garcia
- Institute of Biotechnology, University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560, Brazil
| | - Sandro Tomaz Martins
- Institute of Biotechnology, University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560, Brazil
| | - Janaina da Silva Crespo
- Graduate Program in Materials Science and Engineering (PPGMAT), University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560 Brazil
- Institute of Biotechnology, University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560, Brazil
| | - Carlos A Figueroa
- Graduate Program in Materials Science and Engineering (PPGMAT), University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560 Brazil
| | - Mariana Roesch-Ely
- Institute of Biotechnology, University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560, Brazil
| | - Cesar Aguzzoli
- Graduate Program in Materials Science and Engineering (PPGMAT), University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560 Brazil
- Institute of Biotechnology, University of Caxias do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, Rio Grande do Sul 95070-560, Brazil
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20
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Kitagawa IL, Miyazaki CM, Pitol-Palin L, Okamoto R, de Vasconcellos LMR, Constantino CJL, Lisboa-Filho PN. Titanium-Based Alloy Surface Modification with TiO 2 and Poly(sodium 4-styrenesulfonate) Multilayers for Dental Implants. ACS APPLIED BIO MATERIALS 2021; 4:3055-3066. [PMID: 35014394 DOI: 10.1021/acsabm.0c01348] [Citation(s) in RCA: 15] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/14/2022]
Abstract
Implant placement is an important repair method in dentistry and orthopedics. Increasing efforts have focused on optimizing the biocompatibility and osseointegration properties of titanium (Ti) and Ti-based alloys. In this work, Ti-based alloys were modified by the layer-by-layer (LbL) technique, which is a simple and versatile method for surface modification. The morphology and chemical structure of LbL films of poly(sodium 4-styrenesulfonate) (PSS) and Ti dioxide (TiO2) nanoparticles were first characterized employing ultraviolet-visible and Fourier-transform infrared spectroscopies as well as atomic force microscopy for further application in Ti-based alloy implants. The changes provoked by the LbL PSS/TiO2 film on the Ti-based alloy surfaces were then investigated by scanning electron microscopy and micro-Raman techniques. Finally, in vivo tests (immunolabeling and biomechanical analysis) performed with screw implants in rats suggested that PSS/TiO2 multilayers promote changes in both topography and chemical surface properties of the screw, providing beneficial effects for osteoblast activity. This simple and relatively low-cost growth process can open up possibilities to improve dental implants and, probably, bone implants in general.
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Affiliation(s)
- Igor L Kitagawa
- School of Sciences, Department of Physics, UNESP São Paulo State University, Bauru, São Paulo 17033-360, Brazil.,Science and Technology of São Paulo, IFSP Federal Institute of Education, Campus Birigui, Birigui, São Paulo 16201-407, Brazil
| | - Celina M Miyazaki
- CCTS, Federal University of São Carlos, Sorocaba, São Paulo 18052-780, Brazil
| | - Letícia Pitol-Palin
- Araçatuba Dental School, Department of Basic Science, UNESP São Paulo State University, Araçatuba 16018-805, Brazil
| | - Roberta Okamoto
- Araçatuba Dental School, Department of Basic Science, UNESP São Paulo State University, Araçatuba 16018-805, Brazil
| | - Luana M R de Vasconcellos
- Institute of Science and Technology, Department of Biosciences and Oral Diagnosis, UNESP São Paulo State University, São José dos Campos, São Paulo 12245-000, Brazil
| | - Carlos J L Constantino
- School of Technology and Applied Sciences, Department of Physics, UNESP São Paulo State University, Presidente Prudente, São Paulo 19060-900, Brazil
| | - Paulo N Lisboa-Filho
- School of Sciences, Department of Physics, UNESP São Paulo State University, Bauru, São Paulo 17033-360, Brazil
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21
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Le PTM, Shintani SA, Takadama H, Ito M, Kakutani T, Kitagaki H, Terauchi S, Ueno T, Nakano H, Nakajima Y, Inoue K, Matsushita T, Yamaguchi S. Bioactivation Treatment with Mixed Acid and Heat on Titanium Implants Fabricated by Selective Laser Melting Enhances Preosteoblast Cell Differentiation. NANOMATERIALS (BASEL, SWITZERLAND) 2021; 11:987. [PMID: 33921268 PMCID: PMC8069428 DOI: 10.3390/nano11040987] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/24/2021] [Revised: 04/09/2021] [Accepted: 04/09/2021] [Indexed: 12/17/2022]
Abstract
Selective laser melting (SLM) is a promising technology capable of producing individual characteristics with a high degree of surface roughness for implants. These surfaces can be modified so as to increase their osseointegration, bone generation and biocompatibility, features which are critical to their clinical success. In this study, we evaluated the effects on preosteoblast proliferation and differentiation of titanium metal (Ti) with a high degree of roughness (Ra = 5.4266 ± 1.282 µm) prepared by SLM (SLM-Ti) that was also subjected to surface bioactive treatment by mixed acid and heat (MAH). The results showed that the MAH treatment further increased the surface roughness, wettability and apatite formation capacity of SLM-Ti, features which are useful for cell attachment and bone bonding. Quantitative measurement of osteogenic-related gene expression by RT-PCR indicated that the MC3T3-E1 cells on the SLM-Ti MAH surface presented a stronger tendency towards osteogenic differentiation at the genetic level through significantly increased expression of Alp, Ocn, Runx2 and Opn. We conclude that bio-activated SLM-Ti enhanced preosteoblast differentiation. These findings suggest that the mixed acid and heat treatment on SLM-Ti is promising method for preparing the next generation of orthopedic and dental implants because of its apatite formation and cell differentiation capability.
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Affiliation(s)
- Phuc Thi Minh Le
- Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan; (S.A.S.); (H.T.); (M.I.); (T.M.)
| | - Seine A. Shintani
- Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan; (S.A.S.); (H.T.); (M.I.); (T.M.)
| | - Hiroaki Takadama
- Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan; (S.A.S.); (H.T.); (M.I.); (T.M.)
| | - Morihiro Ito
- Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan; (S.A.S.); (H.T.); (M.I.); (T.M.)
| | - Tatsuya Kakutani
- Osaka Yakin Kogyo Co., Ltd., Zuiko 4-4-28, Higashi Yodogawa-ku, Osaka City, Osaka 533-0005, Japan; (T.K.); (H.K.); (S.T.)
| | - Hisashi Kitagaki
- Osaka Yakin Kogyo Co., Ltd., Zuiko 4-4-28, Higashi Yodogawa-ku, Osaka City, Osaka 533-0005, Japan; (T.K.); (H.K.); (S.T.)
| | - Shuntaro Terauchi
- Osaka Yakin Kogyo Co., Ltd., Zuiko 4-4-28, Higashi Yodogawa-ku, Osaka City, Osaka 533-0005, Japan; (T.K.); (H.K.); (S.T.)
| | - Takaaki Ueno
- Department of Dentistry and Oral Surgery, Division of Medicine for Function and Morphology of Sensor Organ, Faculty of Medicine, Dentistry and Oral Surgery, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki City, Osaka 569-8686, Japan; (T.U.); (H.N.); (Y.N.); (K.I.)
| | - Hiroyuki Nakano
- Department of Dentistry and Oral Surgery, Division of Medicine for Function and Morphology of Sensor Organ, Faculty of Medicine, Dentistry and Oral Surgery, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki City, Osaka 569-8686, Japan; (T.U.); (H.N.); (Y.N.); (K.I.)
| | - Yoichiro Nakajima
- Department of Dentistry and Oral Surgery, Division of Medicine for Function and Morphology of Sensor Organ, Faculty of Medicine, Dentistry and Oral Surgery, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki City, Osaka 569-8686, Japan; (T.U.); (H.N.); (Y.N.); (K.I.)
| | - Kazuya Inoue
- Department of Dentistry and Oral Surgery, Division of Medicine for Function and Morphology of Sensor Organ, Faculty of Medicine, Dentistry and Oral Surgery, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki City, Osaka 569-8686, Japan; (T.U.); (H.N.); (Y.N.); (K.I.)
| | - Tomiharu Matsushita
- Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan; (S.A.S.); (H.T.); (M.I.); (T.M.)
| | - Seiji Yamaguchi
- Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan; (S.A.S.); (H.T.); (M.I.); (T.M.)
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22
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Influence of Three Dental Implant Surfaces on Cell Viability and Bone Behavior. An In Vitro and a Histometric Study in a Rabbit Model. APPLIED SCIENCES-BASEL 2020. [DOI: 10.3390/app10144790] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Abstract
The chemical composition and the surface characteristics of dental implants are factors that have a decisive effect on the osseointegration process. The surface characterization at the compositional and topographic level of three dental implants available in the market was performed with different surface treatments: (1) sandblasted and acid etched surface (SLA), (2) hydroxyapatite (HA) and tricalcium phosphate (TCP) blasted surface (HA/TCP), and (3) HA-blasted and non-etching acid washed surface (HA + AW). In addition, an in vitro viability study of MG-63 osteoblast cells was performed with a JC-1 test. To complete the study, an in vivo study was conducted in New Zealand rabbits. The study analyzed the histometric characteristics of the bone formed around the implants at the level of area, volume, bone density, accumulated bone density, and bone–implant contact (BIC). The rabbits were sacrificed at 6 weeks after implants were placed in the tibial metaphysis. No statistically significant differences were observed at the level of cell viability or histometric parameters between the different study groups (p > 0.05). SLA and HA/TCP surfaces were the ones that obtained a higher BIC value. Taking into account the limitations of this study, it can be concluded that the different implant surfaces analyzed favor a good bone response.
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23
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Kim JH, Kim MY, Knowles JC, Choi S, Kang H, Park SH, Park SM, Kim HW, Park JT, Lee JH, Lee HH. Mechanophysical and biological properties of a 3D-printed titanium alloy for dental applications. Dent Mater 2020; 36:945-958. [DOI: 10.1016/j.dental.2020.04.027] [Citation(s) in RCA: 23] [Impact Index Per Article: 4.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/12/2020] [Revised: 04/20/2020] [Accepted: 04/30/2020] [Indexed: 12/22/2022]
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24
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Scandurra R, Scotto d’Abusco A, Longo G. A Review of the Effect of a Nanostructured Thin Film Formed by Titanium Carbide and Titanium Oxides Clustered around Carbon in Graphitic Form on Osseointegration. NANOMATERIALS (BASEL, SWITZERLAND) 2020; 10:E1233. [PMID: 32599955 PMCID: PMC7353133 DOI: 10.3390/nano10061233] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 05/10/2020] [Revised: 06/15/2020] [Accepted: 06/21/2020] [Indexed: 11/30/2022]
Abstract
Improving the biocompatibility of implants is an extremely important step towards improving their quality. In this review, we recount the technological and biological process for coating implants with thin films enriched in titanium carbide (TiC), which provide improved cell growth and osseointegration. At first, we discuss the use of a Pulsed Laser Ablation Deposition, which produced films with a good biocompatibility, cellular stimulation and osseointegration. We then describe how Ion Plating Plasma Assisted technology could be used to produce a nanostructured layer composed by graphitic carbon, whose biocompatibility is enhanced by titanium oxides and titanium carbide. In both cases, the nanostructured coating was compact and strongly bound to the bulk titanium, thus particularly useful to protect implants from the harsh oxidizing environment of biological tissues. The morphology and chemistry of the nanostructured coating were particularly desirable for osteoblasts, resulting in improved proliferation and differentiation. The cellular adhesion to the TiC-coated substrates was much stronger than to uncoated surfaces, and the number of philopodia and lamellipodia developed by the cells grown on the TiC-coated samples was higher. Finally, tests performed on rabbits confirmed in vivo that the osseointegration process of the TiC-coated implants is more efficient than that of uncoated titanium implants.
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Affiliation(s)
- Roberto Scandurra
- Department of Biochemical Sciences, Sapienza University of Roma, Piazzale A. Moro 5, 00185 Roma, Italy;
| | - Anna Scotto d’Abusco
- Department of Biochemical Sciences, Sapienza University of Roma, Piazzale A. Moro 5, 00185 Roma, Italy;
| | - Giovanni Longo
- Consiglio Nazionale delle Ricerche-Istituto di Struttura della Materia, Via del Fosso del Cavaliere, 00133 Roma, Italy;
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25
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Hasegawa M, Saruta J, Hirota M, Taniyama T, Sugita Y, Kubo K, Ishijima M, Ikeda T, Maeda H, Ogawa T. A Newly Created Meso-, Micro-, and Nano-Scale Rough Titanium Surface Promotes Bone-Implant Integration. Int J Mol Sci 2020; 21:ijms21030783. [PMID: 31991761 PMCID: PMC7036846 DOI: 10.3390/ijms21030783] [Citation(s) in RCA: 28] [Impact Index Per Article: 5.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/10/2019] [Revised: 01/22/2020] [Accepted: 01/23/2020] [Indexed: 12/16/2022] Open
Abstract
Titanium implants are the standard therapeutic option when restoring missing teeth and reconstructing fractured and/or diseased bone. However, in the 30 years since the advent of micro-rough surfaces, titanium’s ability to integrate with bone has not improved significantly. We developed a method to create a unique titanium surface with distinct roughness features at meso-, micro-, and nano-scales. We sought to determine the biological ability of the surface and optimize it for better osseointegration. Commercially pure titanium was acid-etched with sulfuric acid at different temperatures (120, 130, 140, and 150 °C). Although only the typical micro-scale compartmental structure was formed during acid-etching at 120 and 130 °C, meso-scale spikes (20–50 μm wide) and nano-scale polymorphic structures as well as micro-scale compartmental structures formed exclusively at 140 and 150 °C. The average surface roughness (Ra) of the three-scale rough surface was 6–12 times greater than that with micro-roughness only, and did not compromise the initial attachment and spreading of osteoblasts despite its considerably increased surface roughness. The new surface promoted osteoblast differentiation and in vivo osseointegration significantly; regression analysis between osteoconductivity and surface variables revealed these effects were highly correlated with the size and density of meso-scale spikes. The overall strength of osseointegration was the greatest when the acid-etching was performed at 140 °C. Thus, we demonstrated that our meso-, micro-, and nano-scale rough titanium surface generates substantially increased osteoconductive and osseointegrative ability over the well-established micro-rough titanium surface. This novel surface is expected to be utilized in dental and various types of orthopedic surgical implants, as well as titanium-based bone engineering scaffolds.
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Affiliation(s)
- Masakazu Hasegawa
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA (M.H.); (M.I.); (T.I.)
- Department of Oral Pathology, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan
| | - Juri Saruta
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA (M.H.); (M.I.); (T.I.)
- Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan
- Correspondence: ; Tel./Fax: +81-46-822-9537
| | - Makoto Hirota
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA (M.H.); (M.I.); (T.I.)
- Department of Oral and Maxillofacial Surgery/Orthodontics, Yokohama City University Medical Center, 4-57 Urafune-cho, Yokohama, Kanagawa 232-0024, Japan
| | - Takashi Taniyama
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA (M.H.); (M.I.); (T.I.)
- Department of Orthopedic Surgery, Yokohama City Minato Red Cross Hospital, 3-12-1 Shinyamashita, Yokohama, Kanagawa 231-8682, Japan
| | - Yoshihiko Sugita
- Department of Oral Pathology, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan
| | - Katsutoshi Kubo
- Department of Oral Pathology, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan
| | - Manabu Ishijima
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA (M.H.); (M.I.); (T.I.)
| | - Takayuki Ikeda
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA (M.H.); (M.I.); (T.I.)
| | - Hatsuhiko Maeda
- Department of Oral Pathology, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan
| | - Takahiro Ogawa
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA (M.H.); (M.I.); (T.I.)
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26
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Le Guéhennec L, Van Hede D, Plougonven E, Nolens G, Verlée B, De Pauw MC, Lambert F. In vitro and in vivo biocompatibility of calcium-phosphate scaffolds three-dimensional printed by stereolithography for bone regeneration. J Biomed Mater Res A 2019; 108:412-425. [PMID: 31654476 DOI: 10.1002/jbm.a.36823] [Citation(s) in RCA: 36] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/21/2019] [Revised: 10/08/2019] [Accepted: 10/11/2019] [Indexed: 12/11/2022]
Abstract
Stereolithography (SLA) is an interesting manufacturing technology to overcome limitations of commercially available particulated biomaterials dedicated to intra-oral bone regeneration applications. The purpose of this study was to evaluate the in vitro and in vivo biocompatibility and osteoinductive properties of two calcium-phosphate (CaP)-based scaffolds manufactured by SLA three-dimensional (3D) printing. Pellets and macro-porous scaffolds were manufactured in pure hydroxyapatite (HA) and in biphasic CaP (HA:60-TCP:40). Physico-chemical characterization was performed using micro X-ray fluorescence, scanning electron microscopy (SEM), optical interferometry, and microtomography (μCT) analyses. Osteoblast-like MG-63 cells were used to evaluate the biocompatibility of the pellets in vitro with MTS assay and the cell morphology and growth characterized by SEM and DAPI-actin staining showed similar early behavior. For in vivo biocompatibility, newly formed bone and biodegradability of the experimental scaffolds were evaluated in a subperiosteal cranial rat model using μCT and descriptive histology. The histological analysis has not indicated evidences of inflammation but highlighted close contacts between newly formed bone and the experimental biomaterials revealing an excellent scaffold osseointegration. This study emphasizes the relevance of SLA 3D printing of CaP-based biomaterials for intra-oral bone regeneration even if manufacturing accuracy has to be improved and further experiments using biomimetic scaffolds should be conducted.
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Affiliation(s)
- Laurent Le Guéhennec
- Department of Prosthetic Dentistry, Faculty of Dentistry, Nantes, France.,Department of Preclinical Biomedical Sciences, Mammalian Cell Culture Laboratory, GIGA-R, Faculty of Medicine, Liège, Belgium
| | - Dorien Van Hede
- Department of Periodontology and Oral Surgery, Faculty of Medicine, Liège, Belgium
| | - Erwan Plougonven
- Department of Chemical Engineering, Faculty of Applied Sciences, Liège, Belgium
| | - Grégory Nolens
- Department of Biomedical Sciences, Faculty of Medicine, Namur, Belgium
| | - Bruno Verlée
- Sirris, Additive Manufacturing Department, Seraing, Belgium
| | - Marie-Claire De Pauw
- Department of Preclinical Biomedical Sciences, Mammalian Cell Culture Laboratory, GIGA-R, Faculty of Medicine, Liège, Belgium
| | - France Lambert
- Department of Periodontology and Oral Surgery, Faculty of Medicine, Liège, Belgium
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27
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Long EG, Buluk M, Gallagher MB, Schneider JM, Brown JL. Human mesenchymal stem cell morphology, migration, and differentiation on micro and nano-textured titanium. Bioact Mater 2019; 4:249-255. [PMID: 31667441 PMCID: PMC6812408 DOI: 10.1016/j.bioactmat.2019.08.001] [Citation(s) in RCA: 33] [Impact Index Per Article: 5.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/29/2019] [Revised: 07/24/2019] [Accepted: 08/26/2019] [Indexed: 01/25/2023] Open
Abstract
Orthopedic implants rely on facilitating a robust interaction between the implant material surface and the surrounding bone tissue. Ideally, the interface will encourage osseointegration with the host bone, resulting in strong fixation and implant stability. However, implant failure can occur due to the lack of integration with bone tissue or bacterial infection. The chosen material and surface topography of orthopedic implants are key factors that influence the early events following implantation and may ultimately define the success of a device. Early attachment, rapid migration and improved differentiation of stem cells to osteoblasts are necessary to populate the surface of biomedical implants, potentially preventing biofilm formation and implant-associated infection. This article explores these early stem cell specific events by seeding human mesenchymal stem cells (MSCs) on four clinically relevant materials: polyether ether ketone (PEEK), Ti6Al4V (smooth Ti), macro-micro rough Ti6Al4V (Endoskeleton®), and macro-micro-nano rough Ti6Al4V (nanoLOCK®). The results demonstrate the incorporation of a hierarchical macro-micro-nano roughness on titanium produces a stellate morphology typical of mature osteoblasts/osteocytes, rapid and random migration, and improved osteogenic differentiation in seeded MSCs. Literature suggests rapid coverage of a surface by stem cells coupled with stimulation of bone differentiation minimizes the opportunity for biofilm formation while increasing the rate of device integration with the surrounding bone tissue.
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Affiliation(s)
- Emily G Long
- Department of Biomedical Engineering, The Pennsylvania State University, 122 CBEB Building, University Park, PA, 16802, USA
| | - Merve Buluk
- Department of Biomedical Engineering, The Pennsylvania State University, 122 CBEB Building, University Park, PA, 16802, USA
| | - Michelle B Gallagher
- Titan Spine, Inc., Mequon Research Center, 6140 W. Executive Drive, Suite A, Mequon, WI, 53092, USA
| | - Jennifer M Schneider
- Titan Spine, Inc., Mequon Research Center, 6140 W. Executive Drive, Suite A, Mequon, WI, 53092, USA
| | - Justin L Brown
- Department of Biomedical Engineering, The Pennsylvania State University, 122 CBEB Building, University Park, PA, 16802, USA
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28
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Mello DDCR, de Oliveira JR, Cairo CAA, Ramos LSDB, Vegian MRDC, de Vasconcellos LGO, de Oliveira FE, de Oliveira LD, de Vasconcellos LMR. Titanium alloys: in vitro biological analyzes on biofilm formation, biocompatibility, cell differentiation to induce bone formation, and immunological response. JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE 2019; 30:108. [PMID: 31535222 DOI: 10.1007/s10856-019-6310-2] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/05/2019] [Accepted: 09/04/2019] [Indexed: 06/10/2023]
Abstract
Biological effects of titanium (Ti) alloys were analyzed on biofilms of Candida albicans, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus mutans, and Streptococcus sanguinis, as well as on osteoblast-like cells (MG63) and murine macrophages (RAW 264.7). Standard samples composed of aluminum and vanadium (Ti-6Al-4V), and sample containing niobium (Ti-35Nb) and zirconium (Ti-13Nb-13Zr) were analyzed. Monomicrobial biofilms were formed on the Ti alloys. MG63 cells were grown with the alloys and the biocompatibility (MTT), total protein (TP) level, alkaline phosphatase (ALP) activity, and mineralization nodules (MN) formation were verified. Levels of interleukins (IL-1β and IL-17), tumor necrosis factor alpha (TNF-α), and oxide nitric (NO) were checked, from RAW 264.7 cells supernatants. Data were statically analyzed by one-way analysis of variance (ANOVA) and Tukey's test, or T-test (P ≤ 0.05). Concerning the biofilm formation, Ti-13Nb-13Zr alloy showed the best inhibitory effect on E. faecalis, P. aeruginosa, and S. aureus. And, it also acted similarly to the Ti-6Al-4V alloy on C. albicans and Streptococcus spp. Both alloys were biocompatible and similar to the Ti-6Al-4V alloy. Additionally, Ti-13Nb-13Zr alloy was more effective for cell differentiation, as observed in the assays of ALP and MN. Regarding the stimulation for release of IL-1β and TNF-α, Ti-35Nb and Ti-13Nb-13Zr alloys inhibited similarly the synthesis of these molecules. However, both alloys stimulated the production of IL-17. Additionally, all Ti alloys showed the same effect for NO generation. Thus, Ti-13Nb-13Zr alloy was the most effective for inhibition of biofilm formation, cell differentiation, and stimulation for release of immune mediators.
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Affiliation(s)
- Daphne de Camargo Reis Mello
- Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, CEP12245-000, Brazil
| | - Jonatas Rafael de Oliveira
- Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, CEP12245-000, Brazil.
| | - Carlos Alberto Alves Cairo
- Division of Materials, Air and Space Institute (CTA), Praça Marechal do Ar Eduardo Gomes, 14, São José dos Campos, SP, CEP 12904-000, Brazil
| | - Lais Siebra de Brito Ramos
- Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, CEP12245-000, Brazil
| | - Mariana Raquel da Cruz Vegian
- Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, CEP12245-000, Brazil
| | - Luis Gustavo Oliveira de Vasconcellos
- Department of Materials and Dental Prosthodontics, Institute of Science and Technology, São Paulo State University (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, CEP12245-000, Brazil
| | - Felipe Eduardo de Oliveira
- Brazcubas Faculty of Dentistry, University Center Brazcubas, Av. Francisco Rodrigues Filho, 1233, Mogi das Cruzes, SP, CEP 08773-380, Brazil
| | - Luciane Dias de Oliveira
- Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, CEP12245-000, Brazil
| | - Luana Marotta Reis de Vasconcellos
- Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), Av. Engenheiro Francisco José Longo, 777, São José dos Campos, SP, CEP12245-000, Brazil
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Teng FY, Tai IC, Ho ML, Wang JW, Weng LW, Wang YJ, Wang MW, Tseng CC. Controlled release of BMP-2 from titanium with electrodeposition modification enhancing critical size bone formation. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2019; 105:109879. [PMID: 31546456 DOI: 10.1016/j.msec.2019.109879] [Citation(s) in RCA: 33] [Impact Index Per Article: 5.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/16/2018] [Revised: 05/09/2019] [Accepted: 06/10/2019] [Indexed: 02/07/2023]
Abstract
In this study, a porous Ti-alloy based implant with an interconnected channel structure (MAO-CaP-BMP2) is fabricated using a method combining 3D printing, microarc oxidation (MAO) treatment, and co-precipitation of Ca,P layer with BMP-2 technique. The macroporous structure with pore size of 600 μm made by 3D printing not only enhances the ingrowth of cells but also allows the formation of blood vessels inside the implant. As a result, the new bond formation is promoted. In addition, the microporous dioxide layer formed on the implant surface by MAO provides the sites for co-precipitation of Ca,P layer with BMP-2. The microstructure allows the prolonged release of BMP-2. Our results show that a sustained release of BMP-2 over 35 days is achieved for MAO-CaP-BMP2 group longer than Ti without MAO modification group and without Ca,P electrochemical deposition group. The slow release of BMP-2 at the bone/implant interface for a long period of time leads to enhancement of the osseointegration between the implant and surrounding bones. This result indicates that MAO-CaP-BMP2 is a good candidate of growth factor carrier. Successful regeneration of bone requires the concomitant processes of osteogenesis and neovascularization. MAO-CaP-BMP2 modified Ti-alloy implant is both osteoinductive and osteoconductive which can create better osteogenesis and angiogenesis. As a result, it can enhance bone formation.
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Affiliation(s)
- Fu-Yuan Teng
- Department of Dentistry, Koo Foundation Sun Yat-Sen Cancer Center, Taipei City, Taiwan
| | - I-Chun Tai
- Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
| | - Mei-Ling Ho
- Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
| | - Jhe-Wen Wang
- Department of Mechanical Engineering, Graduate Institute of Mechanical and Precision Engineering, National Kaohsiung University of Applied Sciences, Kaohsiung, Taiwan
| | - Li Wen Weng
- Medical Device Section, Medical Devices and Opto-Electronics Equipment Department, Metal Industries Research & Development Centre, Kaohsiung, Taiwan
| | - Yue Jun Wang
- Medical Device Section, Medical Devices and Opto-Electronics Equipment Department, Metal Industries Research & Development Centre, Kaohsiung, Taiwan
| | - Min-Wen Wang
- Department of Mechanical Engineering, Graduate Institute of Mechanical and Precision Engineering, National Kaohsiung University of Applied Sciences, Kaohsiung, Taiwan.
| | - Chun-Chieh Tseng
- Medical Device Section, Medical Devices and Opto-Electronics Equipment Department, Metal Industries Research & Development Centre, Kaohsiung, Taiwan.
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Pansani TN, Basso FG, Souza IDR, Hebling J, de Souza Costa CA. Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts. Arch Oral Biol 2019; 102:48-54. [PMID: 30965181 DOI: 10.1016/j.archoralbio.2019.03.025] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/19/2018] [Revised: 03/12/2019] [Accepted: 03/28/2019] [Indexed: 11/18/2022]
Abstract
OBJECTIVES Different strategies, such as modifications on the implant abutments surface have been proposed to accelerate and improve the formation of the biological seal (BS). The aim of this study was to characterize a titanium (Ti) surface impregnated with epidermal growth factor (EGF) and to assess its influence on the metabolism and adhesion of oral mucosal cells. DESIGN Ti discs were coated with EGF (100 nM) conjugated with a fluorophore and analyzed by fluorescence microscopy. The surface roughness analysis (Ra) of the EGF-coated Ti was performed by confocal microscopy. The EGF released in the wet environment was determined at 0, 24, 48 and 72 h by fluorimetric quantification. For assessment of the biological effects of EGF-coated Ti, gingival fibroblasts were seeded (5 × 104 cells) onto the substrate coated or not with this growth factor. After 24 h, cell adhesion and viability were evaluated by ANOVA and Tukey tests, α = .05. RESULTS Immediate release of EGF as well as its incorporation by fibroblasts within 1 h after cells were seeded was observed. EGF-coated Ti discs presented significantly enhance surface roughness. Increased cell viability was observed on the EGF-coated discs. CONCLUSION EGF applied to Ti discs stimulated the adhesion and metabolism of gingival fibroblasts and could be considered as an interesting alternative for improving the BS.
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Affiliation(s)
- Taisa Nogueira Pansani
- Department of Dental Materials and Prosthodontics, UNESP - Univ. Estadual Paulista, Araraquara School of Dentistry, Araraquara, Brazil
| | | | - Isabela Dos Reis Souza
- Department of Dental Materials and Prosthodontics, UNESP - Univ. Estadual Paulista, Araraquara School of Dentistry, Araraquara, Brazil
| | - Josimeri Hebling
- Department of Orthodontics and Pediatric Dentistry, UNESP - Univ. Estadual Paulista, Araraquara School of Dentistry, Araraquara, Brazil
| | - Carlos Alberto de Souza Costa
- Department of Physiology and Pathology, UNESP - Univ. Estadual Paulista, Araraquara School of Dentistry, Araraquara, Brazil.
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Radtke A, Ehlert M, Jędrzejewski T, Bartmański M. The Morphology, Structure, Mechanical Properties and Biocompatibility of Nanotubular Titania Coatings before and after Autoclaving Process. J Clin Med 2019; 8:E272. [PMID: 30813448 PMCID: PMC6406720 DOI: 10.3390/jcm8020272] [Citation(s) in RCA: 17] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/29/2019] [Revised: 02/20/2019] [Accepted: 02/20/2019] [Indexed: 12/18/2022] Open
Abstract
The autoclaving process is one of the sterilization procedures of implantable devices. Therefore, it is important to assess the impact of hot steam at high pressure on the morphology, structure, and properties of implants modified by nanocomposite coatings. In our works, we focused on studies on amorphous titania nanotubes produced by titanium alloy (Ti6Al4V) electrochemical oxidation in the potential range 5⁻60 V. Half of the samples were drying in argon stream at room temperature, and the second ones were drying additionally with the use of immersion in acetone and drying at 396 K. Samples were subjected to autoclaving and after sterilization they were structurally and morphologically characterized using Raman spectroscopy, diffuse reflectance infrared Fourier transform spectroscopy (DRIFT) and scanning electron microscopy (SEM). They were characterized in terms of wettability, mechanical properties, and biocompatibility. Obtained results proved that the autoclaving of amorphous titania nanotube coatings produced at lower potentials (5⁻15 V) does not affect their morphology and structure regardless of the drying method before autoclaving. Nanotubular coatings produced using higher potentials (20⁻60 V) require removal of adsorbed water particles from their surface. Otherwise, autoclaving leads to the destruction of the architecture of nanotubular coatings, which is associated with the changing of their mechanical and biointegration properties.
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Affiliation(s)
- Aleksandra Radtke
- Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, Poland.
- Nano-implant Ltd., Gagarina 5/102, 87-100 Toruń, Poland.
| | - Michalina Ehlert
- Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, Poland.
- Nano-implant Ltd., Gagarina 5/102, 87-100 Toruń, Poland.
| | - Tomasz Jędrzejewski
- Faculty of Biology and Environmental Protection, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland.
| | - Michał Bartmański
- Faculty of Mechanical Engineering, Gdańsk University of Technology, Gabriela Narutowicza 11/12, 80-233 Gdańsk, Poland.
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Komasa S, Nishizaki M, Zhang H, Takao S, Yin D, Terada C, Kobayashi Y, Kusumoto T, Yoshimine S, Nishizaki H, Okazaki J, Chen L. Osseointegration of Alkali-Modified NANOZR Implants: An In Vivo Study. Int J Mol Sci 2019; 20:ijms20040842. [PMID: 30781372 PMCID: PMC6413168 DOI: 10.3390/ijms20040842] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/17/2019] [Revised: 02/07/2019] [Accepted: 02/12/2019] [Indexed: 11/24/2022] Open
Abstract
Ingredients and surface modification methods are being continually developed to improve osseointegration of dental implants and reduce healing times. In this study, we demonstrate in vitro that, by applying concentrated alkali treatment to NANOZR with strong bending strength and fracture toughness, a significant improvement in the bone differentiation of rat bone marrow cells can be achieved. We investigated the influence of materials modified with this treatment in vivo, on implanted surrounding tissues using polychrome sequential fluorescent labeling and micro-computer tomography scanning. NANOZR implant screws in the alkali-treated group and the untreated group were evaluated after implantation in the femur of Sprague–Dawley male rats, indicating that the amount of new bone in the alkali-modified NANOZR was higher than that of unmodified NANOZR. Alkali-modified NANOZR implants proved to be useful for the creation of new implant materials.
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Affiliation(s)
- Satoshi Komasa
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 5731121, Japan.
| | - Mariko Nishizaki
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 5731121, Japan.
| | - Honghao Zhang
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 5731121, Japan.
| | - Seiji Takao
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 5731121, Japan.
| | - Derong Yin
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 5731121, Japan.
| | - Chisato Terada
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 5731121, Japan.
| | - Yasuyuki Kobayashi
- Osaka Research Institute of Industrial Science and Technology Morinomiya Center, 1-6-50, Morinomiya, Joto-ku, Osaka-shi 5368553, Japan.
| | - Tetsuji Kusumoto
- Osaka Dental University Japan Faculty of Health Sciences, 1-4-4, Makino-honmachi, Hirakata-shi, Osaka 5731144, Japan.
| | - Shigeki Yoshimine
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 5731121, Japan.
| | - Hiroshi Nishizaki
- Osaka Dental University Japan Faculty of Health Sciences, 1-4-4, Makino-honmachi, Hirakata-shi, Osaka 5731144, Japan.
| | - Joji Okazaki
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 5731121, Japan.
| | - Luyuan Chen
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 5731121, Japan.
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Montazeri M, Hashemi A, Houshmand B, Faghihi S. The Effect of Bio-Conditioning of Titanium Implants for Enhancing Osteogenic Activity. J ORAL IMPLANTOL 2019; 45:187-195. [PMID: 30702957 DOI: 10.1563/aaid-joi-d-18-00020] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
Early and effective integration of titanium-based materials into bone tissue is of vital importance for long-term stability of implants. Surface modification is commonly used to enhance cell-substrate interactions for improving cell adhesion, proliferation, and activity. Here, the surface of titanium substrates and commercial implants were coated with blood (TiB), fetal bovine serum (TiF), and phosphate-buffered saline (TiP) solution using a spin coating process. Surface roughness and wettability of samples were measured using contact angle measurements and atomic force microscopy. The samples were then exposed to human osteoblast-like MG63 cells in order to evaluate adhesion, growth, differentiation, and morphology on the surface of modified samples. Untreated titanium disks were used as controls. The lowest roughness and wettability values were found in unmodified titanium samples followed by TiP, TiF, and TiB. The percentage of cellular attachment and proliferation for each sample was measured using an MTT (3-[4,5-dimethylthiazol-2yl] 2,5diphenyl-2H-tetrazoliumbromide) assay. Cell adhesion and proliferation were most improved on TiB followed closely by TiF. The results of this study revealed an increased expression of the osteogenic marker protein alkaline phosphatase on TiB and the coated commercial titanium implants. These results suggested that precoating titanium samples with blood may improve cellular response by successfully mimicking a physiological environment that could be beneficial for clinical implant procedures.
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Affiliation(s)
- Mohadeseh Montazeri
- 1 Stem Cell and Regenerative Medicine Group, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
| | - Amir Hashemi
- 2 Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran. Iran
| | - Behzad Houshmand
- 3 Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Shahab Faghihi
- 1 Stem Cell and Regenerative Medicine Group, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
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Identification of Key Signaling Pathways Orchestrating Substrate Topography Directed Osteogenic Differentiation Through High-Throughput siRNA Screening. Sci Rep 2019; 9:1001. [PMID: 30700820 PMCID: PMC6353928 DOI: 10.1038/s41598-018-37554-y] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/19/2018] [Accepted: 12/04/2018] [Indexed: 12/14/2022] Open
Abstract
Fibrous scaffolds are used for bone tissue engineering purposes with great success across a variety of polymers with different physical and chemical properties. It is now evident that the correct degree of curvature promotes increased cytoskeletal tension on osteoprogenitors leading to osteogenic differentiation. However, the mechanotransductive pathways involved in this phenomenon are not fully understood. To achieve a reproducible and specific cellular response, an increased mechanistic understanding of the molecular mechanisms driving the fibrous scaffold mediated bone regeneration must be understood. High throughput siRNA mediated screening technology has been utilized for dissecting molecular targets that are important in certain cellular phenotypes. In this study, we used siRNA mediated gene silencing to understand the osteogenic differentiation observed on fibrous scaffolds. A high-throughput siRNA screen was conducted using a library collection of 863 genes including important human kinase and phosphatase targets on pre-osteoblast SaOS-2 cells. The cells were grown on electrospun poly(methyl methacrylate) (PMMA) scaffolds with a diameter of 0.938 ± 0.304 µm and a flat surface control. The osteogenic transcription factor RUNX2 was quantified with an in-cell western (ICW) assay for the primary screen and significant targets were selected via two sample t-test. After selecting the significant targets, a secondary screen was performed to identify osteoinductive markers that also effect cell shape on fibrous topography. Finally, we report the most physiologically relevant molecular signaling mechanisms that are involved in growth factor free, fibrous topography mediated osteoinduction. We identified GTPases, membrane channel proteins, and microtubule associated targets that promote an osteoinductive cell shape on fibrous scaffolds.
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35
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Müllers Y, Meiser I, Stracke F, Riemann I, Lautenschläger F, Neubauer JC, Zimmermann H. Quantitative analysis of F-actin alterations in adherent human mesenchymal stem cells: Influence of slow-freezing and vitrification-based cryopreservation. PLoS One 2019; 14:e0211382. [PMID: 30682146 PMCID: PMC6347223 DOI: 10.1371/journal.pone.0211382] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/13/2018] [Accepted: 01/11/2019] [Indexed: 01/09/2023] Open
Abstract
Cryopreservation is an essential tool to meet the increasing demand for stem cells in medical applications. To ensure maintenance of cell function upon thawing, the preservation of the actin cytoskeleton is crucial, but so far there is little quantitative data on the influence of cryopreservation on cytoskeletal structures. For this reason, our study aims to quantitatively describe cryopreservation induced alterations to F-actin in adherent human mesenchymal stem cells, as a basic model for biomedical applications. Here we have characterised the actin cytoskeleton on single-cell level by calculating the circular standard deviation of filament orientation, F-actin content, and average filament length. Cryo-induced alterations of these parameters in identical cells pre and post cryopreservation provide the basis of our investigation. Differences between the impact of slow-freezing and vitrification are qualitatively analyzed and highlighted. Our analysis is supported by live cryo imaging of the actin cytoskeleton via two photon microscopy. We found similar actin alterations in slow-frozen and vitrified cells including buckling of actin filaments, reduction of F-actin content and filament shortening. These alterations indicate limited functionality of the respective cells. However, there are substantial differences in the frequency and time dependence of F-actin disruptions among the applied cryopreservation strategies; immediately after thawing, cytoskeletal structures show least disruption after slow freezing at a rate of 1°C/min. As post-thaw recovery progresses, the ratio of cells with actin disruptions increases, particularly in slow frozen cells. After 120 min of recovery the proportion of cells with an intact actin cytoskeleton is higher in vitrified than in slow frozen cells. Freezing at 10°C/min is associated with a high ratio of impaired cells throughout the post-thawing culture.
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Affiliation(s)
- Yannik Müllers
- Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany
| | - Ina Meiser
- Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany
| | - Frank Stracke
- Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany
| | - Iris Riemann
- Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany
| | - Franziska Lautenschläger
- Division of Cytoskeletal Fibers, Leibniz Institute for New Materials, Campus D2 2, Saarbrücken, Germany
- Chair for Experimental Physics, Saarland University, Saarbrücken, Germany
| | - Julia C. Neubauer
- Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany
- Fraunhofer Project Centre for Stem Cell Process Engineering, Neunerplatz 2, Würzburg, Germany
| | - Heiko Zimmermann
- Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany
- Chair for Molecular and Cellular Biotechnology, Saarland University, Saarbruecken, Germany
- Faculty of Marine Science, Universidad Católica del Norte, Coquimbo, Chile
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Soleymaniha M, Shahbazi MA, Rafieerad AR, Maleki A, Amiri A. Promoting Role of MXene Nanosheets in Biomedical Sciences: Therapeutic and Biosensing Innovations. Adv Healthc Mater 2019; 8:e1801137. [PMID: 30362268 DOI: 10.1002/adhm.201801137] [Citation(s) in RCA: 182] [Impact Index Per Article: 30.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/12/2018] [Indexed: 01/04/2023]
Abstract
MXene nanosheets have emerged as biocompatible transition metal structures, which illustrate desirable performance for various applications due to their unique structural, physicochemical, and compositional features. MXenes are currently expanding their usage territory from mechanical, optical, chemical, and electronic fields toward biomedical areas. This is mainly originated from their large surface area and strong absorbance in near-infrared region, which in combination with their facile surface functionalization with various polymers or nanoparticles, make them promising nanoplatforms for drug delivery, cancer therapy, precise biosensing and bioimaging. The facile surface modification of the MXenes can mediate the better in vivo performance of them through reduced toxicity, enhanced colloidal stability, and extended circulation within the body. Herein, the synthesis and state-of-the-art progresses of MXene nanosheets designed for biomedical applications, including structural- and dose-dependent antimicrobial activity, photothermal therapy, drug delivery, and implants are emphasized. Furthermore, biosensing applications are highlighted and a comprehensive discussion on photoacoustic imaging, magnetic resonance imaging, computed tomography imaging, and optical imaging of MXenes is presented. The challenges and future opportunities of applying MXene nanomaterials in the area of biomedicine are also discussed.
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Affiliation(s)
| | - Mohammad-Ali Shahbazi
- Department of Micro- and Nanotechnology; Technical University of Denmark; Ørsteds Plads DK-2800 Kgs, Lyngby Denmark
- Department of Pharmaceutical Nanotechnology; School of Pharmacy; Zanjan University of Medical Sciences; 45139-56184 Zanjan Iran
- Drug Research Program; Division of Pharmaceutical Chemistry and Technology; Faculty of Pharmacy; University of Helsinki; Helsinki FI-00014 Finland
| | - Ali Reza Rafieerad
- St. Boniface Hospital Research Centre; Department of Physiology; University of Manitoba; Winnipeg Canada
| | - Aziz Maleki
- Department of Micro- and Nanotechnology; Technical University of Denmark; Ørsteds Plads DK-2800 Kgs, Lyngby Denmark
- Zanjan Pharmaceutical Nanotechnology Research Center (ZPNRC); Zanjan University of Medical Sciences; 45139-56184 Zanjan Iran
| | - Ahmad Amiri
- Department of Mechanical Engineering; Texas A&M University; College Station TX 77483 USA
- Department of Pharmaceutical Nanotechnology; School of Pharmacy; Zanjan University of Medical Sciences; 45139-56184 Zanjan Iran
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Abstract
The topography, chemical features, surface charge, and hydrophilic nature of titanium implant surfaces are crucial factors for successful osseointegration. This study aimed to investigate the bone implant contact (BIC) ratio of titanium dental implants with different surface modification techniques using the rat femoral bone model. Sandblasted and acid washed (SL-AW), sandblasted (SL), resorbable blast material (RBM), microarc (MA), and sandblasted and microarc (SL-MA) surfaces were compared in this study. Forty male Sprague-Dawley rats were used in this study. The rats were divided into 5 equal groups (n = 8), and totally 40 implants were integrated into the right femoral bones of the rats. The rats were sacrificed 12 weeks after the surgical integration of the implants. The implant surface-bone tissue interaction was directly observed by a light microscope, and BIC ratios were measured after the nondecalcified histological procedures. Bone implant contact ratios were determined as follows: SL-AW: 59.26 ± 14.36%, SL: 66.01 ± 9.63%, RBM: 63.53 ± 11.23%, MA: 65.51 ± 10.3%, and SL-MA: 68.62 ± 6.6%. No statistically significant differences were found among the 5 different surfaced titanium implant groups (P > 0.05). Our results show that various implant surface modification techniques can provide favorable bone responses to the BIC of dental implants.
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Proliferation of Osteoblasts on Laser-Modified Nanostructured Titanium Surfaces. MATERIALS 2018; 11:ma11101827. [PMID: 30261588 PMCID: PMC6213816 DOI: 10.3390/ma11101827] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 08/30/2018] [Revised: 09/22/2018] [Accepted: 09/25/2018] [Indexed: 12/19/2022]
Abstract
Nanostructured titanium has become a useful material for biomedical applications such as dental implants. Certain surface properties (grain size, roughness, wettability) are highly expected to promote cell adhesion and osseointegration. The aim of this study was to compare the biocompatibilities of several titanium materials using human osteoblast cell line hFOB 1.19. Eight different types of specimens were examined: machined commercially pure grade 2 (cpTi2) and 4 (cpTi4) titanium, nanostructured titanium of the same grades (nTi2, nTi4), and corresponding specimens with laser-treated surfaces (cpTi2L, cpTi4L, nTi2L, nTi4L). Their surface topography was evaluated by means of scanning electron microscopy. Surface roughness was measured using a mechanical contact profilometer. Specimens with laser-treated surfaces had significantly higher surface roughness. Wettability was measured by the drop contact angle method. Nanostructured samples had significantly higher wettability. Cell proliferation after 48 hours from plating was assessed by viability and proliferation assay. The highest proliferation of osteoblasts was found in nTi4 specimens. The analysis of cell proliferation revealed a difference between machined and laser-treated specimens. The mean proliferation was lower on the laser-treated titanium materials. Although plain laser treatment increases surface roughness and wettability, it does not seem to lead to improved biocompatibility.
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Rezaei NM, Hasegawa M, Ishijima M, Nakhaei K, Okubo T, Taniyama T, Ghassemi A, Tahsili T, Park W, Hirota M, Ogawa T. Biological and osseointegration capabilities of hierarchically (meso-/micro-/nano-scale) roughened zirconia. Int J Nanomedicine 2018; 13:3381-3395. [PMID: 29922058 PMCID: PMC5997135 DOI: 10.2147/ijn.s159955] [Citation(s) in RCA: 57] [Impact Index Per Article: 8.1] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/12/2022] Open
Abstract
PURPOSE Zirconia is a potential alternative to titanium for dental and orthopedic implants. Here we report the biological and bone integration capabilities of a new zirconia surface with distinct morphology at the meso-, micro-, and nano-scales. METHODS Machine-smooth and roughened zirconia disks were prepared from yttria-stabilized tetragonal zirconia polycrystal (Y-TZP), with rough zirconia created by solid-state laser sculpting. Morphology of the surfaces was analyzed by three-dimensional imaging and profiling. Rat femur-derived bone marrow cells were cultured on zirconia disks. Zirconia implants were placed in rat femurs and the strength of osseointegration was evaluated by biomechanical push-in test. RESULTS The rough zirconia surface was characterized by meso-scale (50 µm wide, 6-8 µm deep) grooves, micro-scale (1-10 µm wide, 0.1-3 µm deep) valleys, and nano-scale (10-400 nm wide, 10-300 nm high) nodules, whereas the machined surface was flat and uniform. The average roughness (Ra) of rough zirconia was five times greater than that of machined zirconia. The expression of bone-related genes such as collagen I, osteopontin, osteocalcin, and BMP-2 was 7-25 times upregulated in osteoblasts on rough zirconia at the early stage of culture. The number of attached cells and rate of proliferation were similar between machined and rough zirconia. The strength of osseointegration for rough zirconia was twice that of machined zirconia at weeks two and four of healing, with evidence of mineralized tissue persisting around rough zirconia implants as visualized by electron microscopy and elemental analysis. CONCLUSION This unique meso-/micro-/nano-scale rough zirconia showed a remarkable increase in osseointegration compared to machine-smooth zirconia associated with accelerated differentiation of osteoblasts. Cell attachment and proliferation were not compromised on rough zirconia unlike on rough titanium. This is the first report introducing a rough zirconia surface with distinct hierarchical morphology and providing an effective strategy to improve and develop zirconia implants.
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Affiliation(s)
- Naser Mohammadzadeh Rezaei
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA, USA
| | - Masakazu Hasegawa
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA, USA
| | - Manabu Ishijima
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA, USA
| | - Kourosh Nakhaei
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA, USA
| | - Takahisa Okubo
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA, USA
| | - Takashi Taniyama
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA, USA
| | - Amirreza Ghassemi
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA, USA
| | - Tania Tahsili
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA, USA
| | - Wonhee Park
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA, USA
| | - Makoto Hirota
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA, USA
| | - Takahiro Ogawa
- Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of Dentistry, Los Angeles, CA, USA
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Chouirfa H, Evans MDM, Bean P, Saleh-Mghir A, Crémieux AC, Castner DG, Falentin-Daudré C, Migonney V. Grafting of Bioactive Polymers with Various Architectures: A Versatile Tool for Preparing Antibacterial Infection and Biocompatible Surfaces. ACS APPLIED MATERIALS & INTERFACES 2018; 10:1480-1491. [PMID: 29266919 PMCID: PMC5800312 DOI: 10.1021/acsami.7b14283] [Citation(s) in RCA: 24] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/22/2023]
Abstract
The aim of this Research Article is to present three different techniques of poly(sodium styrene sulfonate) (polyNaSS) covalent grafting onto titanium (Ti) surfaces and study the influence of their architecture on biological response. Two of them are "grafting from" techniques requiring an activation step either by thermal or UV irradiation. The third method is a "grafting to" technique involving an anchorage molecule onto which polyNaSS synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization is clicked. The advantage of the "grafting to" technique when compared to the "grafting from" technique is the ability to control the architecture and length of the grafted polymers on the Ti surface and their influence on the biological responses. This investigation compares the effect of the three different grafting processes on the in vitro biological responses of bacteria and osteoblasts. Overall outcomes of this investigation confirmed the significance of the sulfonate functional groups on the biological responses, regardless of the grafting method. In addition, results showed that the architecture and distribution of grafted polyNaSS on Ti surfaces alter the intensity of the bacteria response mediated by fibronectin.
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Affiliation(s)
- Hamza Chouirfa
- LBPS/CSPBAT, UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93340 Villetaneuse, France
| | - Margaret D. M. Evans
- CSIRO Biomedical Materials Manufacturing Program, 11 Julius Avenue, North Ryde, Sydney, NSW 2113, Australia
| | - Penny Bean
- CSIRO Biomedical Materials Manufacturing Program, 11 Julius Avenue, North Ryde, Sydney, NSW 2113, Australia
| | - Azzam Saleh-Mghir
- Département de Médecine Aigüe Spécialisée, Hôpital Universitaire Raymond-Poincaré, Assistance Publique - Hôpitaux de Paris, Garches, and UMR 1173, Faculté de Médecine Paris-Île-de-France Ouest, Université Versailles-Saint-Quentin, Versailles, France
| | - Anne Claude Crémieux
- Département de Médecine Aigüe Spécialisée, Hôpital Universitaire Raymond-Poincaré, Assistance Publique - Hôpitaux de Paris, Garches, and UMR 1173, Faculté de Médecine Paris-Île-de-France Ouest, Université Versailles-Saint-Quentin, Versailles, France
| | - David G. Castner
- National ESCA and Surface Analysis Center for Biomedical Problems, Departments of Bioengineering and Chemical Engineering, University of Washington, Seattle, Washington 98195-1653
| | - Céline Falentin-Daudré
- LBPS/CSPBAT, UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93340 Villetaneuse, France
| | - Véronique Migonney
- LBPS/CSPBAT, UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93340 Villetaneuse, France
- Corresponding author. , LBPS/CSPBAT, UMR CNRS 7244, Institut Galilée, Université Paris 13 Sorbonne Paris Cité, 99 avenue JB Clément 93340-Villetaneuse, France
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Lukaszewska-Kuska M, Wirstlein P, Majchrowski R, Dorocka-Bobkowska B. Osteoblastic cell behaviour on modified titanium surfaces. Micron 2017; 105:55-63. [PMID: 29179009 DOI: 10.1016/j.micron.2017.11.010] [Citation(s) in RCA: 34] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/14/2017] [Revised: 11/22/2017] [Accepted: 11/22/2017] [Indexed: 11/28/2022]
Abstract
INTRODUCTION The surfaces of endoosseous dental implants have been subjected to numerous modifications in order to create a surface which can provide rapid bone healing and fast implant loading. Each modification has involved changes to the chemical composition and topography of the surfaces which have resulted in various biological reactions to the implanted material. AIM The aim of this study was to evaluate the surface topography and chemistry of various modified titanium surfaces: (1) machined surface (MA), (2) alumina-blasted (Al2O3), (3) alumina-blasted and acid-etched (Al2O3 DE), (4) hydroxyapatite/tricalcium phosphate grit-blasted (HA/TCP) and (5) hydroxyapatite/tricalcium phosphate grit-blasted and acid-etched (HA/TCP DE) and to analyse the effects of surface roughness, and chemical composition on human osteoblast vitality, differentiation, morphology and orientation. MATERIALS AND METHODS The modified surfaces were subjected to topographic analysis using Scanning Electron Microscopy (SEM), optical profilometry, roughness analysis and chemical composition evaluation using Energy Dispersion Spectroscopy (EDS) analysis. The biological effects of the titanium modifications was analysed using human osteoblasts cell culture where the cell morphology, vitality (MTS assay) and differentiation (ALP activity) was analysed. RESULTS The machined surfaces were classified as anisotropic, smooth and composed of titanium and oxygen. The blasted surface samples along with the blasted and etched samples were found to be isotropic and rough. The grit-blasting procedure resulted in the incorporation of components from the blasting material. In the case of the blasted and etched samples, etching decreased the surface development as indicated by the Sdr and also reduced the amount of chemical compounds incorporated into the surfaces during the blasting procedure. The attached NHOst cells, proliferated the surfaces. With regard to the MA samples, the cells spread close to the titanium surface, with expanded cytoplasmic extensions and lamelipodia and were oriented in line with the groves left after machining. On the rough substrates, cells were less dispersed and exhibited numerous cytoplasmic extensions, filopodia and interconnections, they were not oriented with respect to the surfaces features. The cell viability of all samples except for Al2O3 decreased after the first day of culture. For all Al2O3, Al2O3 DE and HA samples the viability increased with culture time after an initial reduction. At the end of the culture period the ALP activity was slightly greater on Al2O3 and HA samples compared to the control with the HA DE sample having the same activity as the control. The Al2O3, HA and HA DE ALP samples showed comparable activity and were statistically different from MA and Al2O3 DE samples. CONCLUSIONS In this study, variously treated titanium surfaces were correlated with osteoblastic cell viability, morphology and differentiation in comparison with the plastic and smooth titanium. All examined surfaces were found to be biocompatible. Favourable cell reactions were observed for Al2O3 and HA blasted surfaces. The surface roughness patterns influenced the growth orientation while the surface topography influenced osteoblast morphology. Further animal studies are necessary to compare the in-vivo effect on osseointegration of these modified titanium surfaces.
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Affiliation(s)
- Magdalena Lukaszewska-Kuska
- Department of Gerostomatology and Oral Pathology, Poznan University of Medical Sciences, Bukowska 70, 60-812 Poznan, Poland
| | - Przemysław Wirstlein
- Division of Reproduction, Department of Gynecology and Obstetrics, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, Poland.
| | - Radomir Majchrowski
- Division of Metrology and Measurement Systems, Institute of Mechanical Technology, Poznan University of Technology, Jana Pawła II 24, 60-965 Poznan, Poland.
| | - Barbara Dorocka-Bobkowska
- Department of Gerostomatology and Oral Pathology, Poznan University of Medical Sciences, Bukowska 70, 60-812 Poznan, Poland.
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Boyan BD, Lotz EM, Schwartz Z. * Roughness and Hydrophilicity as Osteogenic Biomimetic Surface Properties. Tissue Eng Part A 2017; 23:1479-1489. [PMID: 28793839 DOI: 10.1089/ten.tea.2017.0048] [Citation(s) in RCA: 97] [Impact Index Per Article: 12.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/09/2023] Open
Abstract
Successful dental and orthopedic implant outcomes are determined by the degree of osseointegration. Over the last 60 years, endosseous implants have evolved to stimulate osteogenesis without the need for exogenous biologics such as bone morphogenetic proteins. An understanding of the interaction between cells and the physical characteristics of their environments has led to development of bioactive implants. Implant surfaces that mimic the inherent chemistry, topography, and wettability of native bone have shown to provide cells in the osteoblast lineage with the structural cues to promote tissue regeneration and net new bone formation. Studies show that attachment, proliferation, differentiation, and local factor production are sensitive to these implant surface characteristics. This review focuses on how surface properties, including chemistry, topography, and hydrophilicity, modulate protein adsorption, cell behavior, biological reactions, and signaling pathways in peri-implant bone tissue, allowing the development of true biomimetics that promote osseointegration by providing an environment suitable for osteogenesis.
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Affiliation(s)
- Barbara D Boyan
- 1 Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University , Richmond, Virginia.,2 Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology , Atlanta, Georgia
| | - Ethan M Lotz
- 1 Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University , Richmond, Virginia
| | - Zvi Schwartz
- 1 Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University , Richmond, Virginia.,3 Department of Periodontics, University of Texas Health Science Center at San Antonio , San Antonio, Texas
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Aluminum Templates of Different Sizes with Micro-, Nano- and Micro/Nano-Structures for Cell Culture. COATINGS 2017. [DOI: 10.3390/coatings7110179] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/16/2022]
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Barthes J, Ciftci S, Ponzio F, Knopf-Marques H, Pelyhe L, Gudima A, Kientzl I, Bognár E, Weszl M, Kzhyshkowska J, Vrana NE. Review: the potential impact of surface crystalline states of titanium for biomedical applications. Crit Rev Biotechnol 2017; 38:423-437. [PMID: 28882077 DOI: 10.1080/07388551.2017.1363707] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Abstract
In many biomedical applications, titanium forms an interface with tissues, which is crucial to ensure its long-term stability and safety. In order to exert control over this process, titanium implants have been treated with various methods that induce physicochemical changes at nano and microscales. In the past 20 years, most of the studies have been conducted to see the effect of topographical and physicochemical changes of titanium surface after surface treatments on cells behavior and bacteria adhesion. In this review, we will first briefly present some of these surface treatments either chemical or physical and we explain the biological responses to titanium with a specific focus on adverse immune reactions. More recently, a new trend has emerged in titanium surface science with a focus on the crystalline phase of titanium dioxide and the associated biological responses. In these recent studies, rutile and anatase are the major two polymorphs used for biomedical applications. In the second part of this review, we consider this emerging topic of the control of the crystalline phase of titanium and discuss its potential biological impacts. More in-depth analysis of treatment-related surface crystalline changes can significantly improve the control over titanium/host tissue interface and can result in considerable decreases in implant-related complications, which is currently a big burden on the healthcare system.
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Affiliation(s)
- Julien Barthes
- a Fundamental Research Unit , Protip Medical , Strasbourg , France.,b INSERM, UMR-S 1121 , , "Biomatériaux et Bioingénierie" , Strasbourg Cedex , France
| | - Sait Ciftci
- b INSERM, UMR-S 1121 , , "Biomatériaux et Bioingénierie" , Strasbourg Cedex , France.,c Service ORL , Hopitaux Universitaires de Strasbourg , Strasbourg , France
| | - Florian Ponzio
- b INSERM, UMR-S 1121 , , "Biomatériaux et Bioingénierie" , Strasbourg Cedex , France.,d Université de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg , Fédération des Matériaux et Nanoscience d'Alsace (FMNA), Faculté de Chirurgie Dentaire , Strasbourg , France
| | - Helena Knopf-Marques
- b INSERM, UMR-S 1121 , , "Biomatériaux et Bioingénierie" , Strasbourg Cedex , France.,d Université de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg , Fédération des Matériaux et Nanoscience d'Alsace (FMNA), Faculté de Chirurgie Dentaire , Strasbourg , France
| | - Liza Pelyhe
- e Department of Materials Science and Engineering, Faculty of Mechanical Engineering , Budapest University of Technology and Economics , Budapest , Hungary
| | - Alexandru Gudima
- f Medical Faculty Mannheim , Institute of Transfusion Medicine and Immunology, University of Heidelberg , Mannheim , Germany
| | - Imre Kientzl
- e Department of Materials Science and Engineering, Faculty of Mechanical Engineering , Budapest University of Technology and Economics , Budapest , Hungary
| | - Eszter Bognár
- e Department of Materials Science and Engineering, Faculty of Mechanical Engineering , Budapest University of Technology and Economics , Budapest , Hungary.,g MTA-BME Research Group for Composite Science and Technology , Budapest , Hungary
| | - Miklós Weszl
- h Department of Biophysics and Radiation Biology , Semmelweis University , Budapest , Hungary
| | - Julia Kzhyshkowska
- f Medical Faculty Mannheim , Institute of Transfusion Medicine and Immunology, University of Heidelberg , Mannheim , Germany.,i German Red Cross Blood Service Baden-Württemberg-Hessen , Mannheim , Germany
| | - Nihal Engin Vrana
- a Fundamental Research Unit , Protip Medical , Strasbourg , France.,b INSERM, UMR-S 1121 , , "Biomatériaux et Bioingénierie" , Strasbourg Cedex , France
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Tsukanaka M, Fujibayashi S, Takemoto M, Matsushita T, Kokubo T, Nakamura T, Sasaki K, Matsuda S. Bioactive treatment promotes osteoblast differentiation on titanium materials fabricated by selective laser melting technology. Dent Mater J 2017; 35:118-25. [PMID: 26830832 DOI: 10.4012/dmj.2015-127] [Citation(s) in RCA: 27] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/23/2022]
Abstract
Selective laser melting (SLM) technology is useful for the fabrication of porous titanium implants with complex shapes and structures. The materials fabricated by SLM characteristically have a very rough surface (average surface roughness, Ra=24.58 µm). In this study, we evaluated morphologically and biochemically the specific effects of this very rough surface and the additional effects of a bioactive treatment on osteoblast proliferation and differentiation. Flat-rolled titanium materials (Ra=1.02 µm) were used as the controls. On the treated materials fabricated by SLM, we observed enhanced osteoblast differentiation compared with the flat-rolled materials and the untreated materials fabricated by SLM. No significant differences were observed between the flat-rolled materials and the untreated materials fabricated by SLM in their effects on osteoblast differentiation. We concluded that the very rough surface fabricated by SLM had to undergo a bioactive treatment to obtain a positive effect on osteoblast differentiation.
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Affiliation(s)
- Masako Tsukanaka
- Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University
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Giannelli M, Bani D, Tani A, Materassi F, Chellini F, Sassoli C. Effects of an Erbium:Yttrium-Aluminum-Garnet Laser and Ultrasonic Scaler on Titanium Dioxide-Coated Titanium Surfaces Contaminated With Subgingival Plaque: An In Vitro Study to Assess Post-Treatment Biocompatibility With Osteogenic Cells. J Periodontol 2017; 88:1211-1220. [PMID: 28691887 DOI: 10.1902/jop.2017.170195] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/13/2022]
Abstract
BACKGROUND Effects of conventional ultrasonic scaler versus an erbium:yttrium-aluminum-garnet (Er:YAG) laser on titanium surfaces contaminated with subgingival plaque from patients with peri-implantitis are evaluated in terms of: 1) plaque and biocorroded titanium oxide coating removal; 2) surface change induction; and 3) residual biocompatibility toward osteoblasts. METHODS Subgingival plaque-coated titanium disks with a moderately rough surface were fixed with ethanol and treated with an ultrasonic scaler (metal tip) or Er:YAG laser (20.3 or 38.2 J/cm2) in non-contact mode. Fluorescent detection of residual plaque was performed. Disk surface morphology was evaluated by scanning electron microscopy. Viability, attachment, proliferation, and differentiation of Saos-2 osteoblasts on new and treated disks were assayed by propidium iodide/DNA stain assay and confocal microscopic analysis of cytoskeleton, Ki67, expression of osteopontin and alkaline phosphatase, and formation of mineralized nodules. RESULTS Both methods resulted in effective debridement of treated surfaces, the plaque area being reduced to 11.7% with the ultrasonic scaler and ≤0.03% with the Er:YAG laser (38.2 J/cm2). Ultrasound-treated disks showed marked surface changes, incomplete removal of the titanium dioxide (TiO2) layer, and scanty plaque aggregates, whereas the Er:YAG laser (38.2 J/cm2) completely stripped away the plaque and TiO2 layer, leaving a micropitted surface. Both treatments maintained a good biocompatibility of surfaces to Saos-2 osteoblasts. Air-water cooling kept disk temperature below the critical threshold of 47°C. CONCLUSION This study shows that an ultrasonic scaler with metal tip is less efficient than high-energy Er:YAG irradiation to remove the plaque and TiO2 layer on anodized disks, although both procedures appear capable of restoring an adequate osseoconductivity of treated surfaces.
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Affiliation(s)
| | - Daniele Bani
- Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Florence, Italy
| | - Alessia Tani
- Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Florence, Italy
| | | | - Flaminia Chellini
- Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Florence, Italy
| | - Chiara Sassoli
- Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Florence, Italy
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Dhaliwal JS, Marulanda J, Li J, Alebrahim S, Feine JS, Murshed M. In vitro comparison of two titanium dental implant surface treatments: 3M™ESPE™ MDIs versus Ankylos®. Int J Implant Dent 2017; 3:27. [PMID: 28656566 PMCID: PMC5487315 DOI: 10.1186/s40729-017-0083-5] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/19/2017] [Accepted: 05/22/2017] [Indexed: 11/19/2022] Open
Abstract
Background An ideal implant should have a surface that is conducive to osseointegration. In vitro cell culture studies using disks made of same materials and surface as of implants may provide useful information on the events occurring at the implant-tissue interface. In the current study, we tested the hypothesis that there is no difference in the proliferation and differentiation capacities of osteoblastic cells when cultured on titanium disks mimicking the surface of 3M™ESPE™ MDIs or standard (Ankylos®) implants. Methods Cells were grown on disks made of the same materials and with same surface texture as those of the original implants. Disks were sterilized and coated with 2% gelatin solution prior to the cell culture experiments. C2C12 pluripotent cells treated with 300 ng/ml bone morphogenetic protein 2 BMP-2 and a stably transfected C2C12 cell line expressing BMP2 were used as models for osteogenic cells. The Hoechst 33258-stained nuclei were counted to assay cell proliferation, while alkaline phosphatase (ALPL) immunostaining was performed to investigate osteogenic differentiation. MC3T3-E1 cells were cultured as model osteoblasts. The cells were differentiated and assayed for proliferation and metabolic activities by Hoechst 33258 staining and Alamar blue reduction assays, respectively. Additionally, cultures were stained by calcein to investigate their mineral deposition properties. Results Electron microscopy showed greater degree of roughness on the MDI surfaces. Nuclear counting showed significantly higher number of C2C12 cells on the MDI surface. Although immunostaining detected higher number of ALPL-positive cells, it was not significant when normalized by cell numbers. The number of MC3T3-E1 cells was also higher on the MDI surface, and accordingly, these cultures showed higher Alamar blue reduction. Finally, calcein staining revealed that the MC3T3-E1 cells grown on MDI surfaces deposited more minerals. Conclusions Although both implant surfaces are conducive for osteoblastic cell attachment, proliferation, and extracellular matrix mineralization, cell proliferation is higher on MDI surfaces, which may in turn facilitate osseointegration via increased ECM mineralization.
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Affiliation(s)
- Jagjit Singh Dhaliwal
- Faculty of Dentistry, McGill University, Montreal, Quebec, Canada.,PAPRSB, Institute of Health Sciences, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE1410, Brunei Darussalam
| | | | - Jingjing Li
- Faculty of Medicine, McGill University, Montreal, Quebec, Canada
| | | | | | - Monzur Murshed
- Faculty of Dentistry, McGill University, Montreal, Quebec, Canada. .,Faculty of Medicine, McGill University, Montreal, Quebec, Canada. .,Shriners Hospital for Children, Montreal, Quebec, H4A 0A9, Canada.
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Bergemann C, Zaatreh S, Wegner K, Arndt K, Podbielski A, Bader R, Prinz C, Lembke U, Nebe JB. Copper as an alternative antimicrobial coating for implants - An in vitro study. World J Transplant 2017; 7:193-202. [PMID: 28698836 PMCID: PMC5487309 DOI: 10.5500/wjt.v7.i3.193] [Citation(s) in RCA: 29] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/28/2016] [Revised: 04/10/2017] [Accepted: 05/15/2017] [Indexed: 02/05/2023] Open
Abstract
AIM To investigate osteoconductive and antimicrobial properties of a titanium-copper-nitride (TiCuN) film and an additional BONIT® coating on titanium substrates.
METHODS For micro-structuring, the surface of titanium test samples was modified by titanium plasma spray (TPS). On the TPS-coated samples, the TiCuN layer was deposited by physical vapor deposition. The BONIT® layer was coated electrochemically. The concentration of copper ions released from TiCuN films was measured by atomic absorption spectrometry. MG-63 osteoblasts on TiCuN and BONIT® were analyzed for cell adhesion, viability and spreading. In parallel, Staphylococcus epidermidis (S. epidermidis) were cultivated on the samples and planktonic and biofilm-bound bacteria were quantified by counting of the colony-forming units.
RESULTS Field emission scanning electron microscopy (FESEM) revealed rough surfaces for TPS and TiCuN and a special crystalline surface structure on TiCuN + BONIT®. TiCuN released high amounts of copper quickly within 24 h. These release dynamics were accompanied by complete growth inhibition of bacteria and after 2 d, no planktonic or adherent S. epidermidis were found on these samples. On the other hand viability of MG-63 cells was impaired during direct cultivation on the samples within 24 h. However, high cell colonization could be found after a 24 h pre-incubation step in cell culture medium simulating the in vivo dynamics closer. On pre-incubated TiCuN, the osteoblasts span the ridges and demonstrate a flattened, well-spread phenotype. The additional BONIT®coating reduced the copper release of the TiCuN layer significantly and showed a positive effect on the initial cell adhesion.
CONCLUSION The TiCuNcoating inhibits the formation of bacterial biofilms on orthopedic implants by influencing the “race for the surface” to the advantage of osteoblasts.
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Nishizaki M, Komasa S, Taguchi Y, Nishizaki H, Okazaki J. Bioactivity of NANOZR Induced by Alkali Treatment. Int J Mol Sci 2017; 18:ijms18040780. [PMID: 28383491 PMCID: PMC5412364 DOI: 10.3390/ijms18040780] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/07/2017] [Revised: 03/28/2017] [Accepted: 04/02/2017] [Indexed: 01/19/2023] Open
Abstract
In recent years, zirconia has been a recognized implant material in clinical dentistry. In the present study, we investigated the performance of an alkali-modified ceria-stabilized tetragonal ZrO2 polycrystalline ceramic-based nanostructured zirconia/alumina composite (NANOZR) implant by assessing surface morphology and composition, wettability, bovine serum albumin adsorption rate, rat bone marrow (RBM) cell attachment, and capacity for inducing bone differentiation. NANOZR surfaces without and with alkali treatment served as the control and test groups, respectively. RBM cells were seeded in a microplate with the implant and cultured in osteogenic differentiation medium, and their differentiation was evaluated by measuring alkaline phosphatase (ALP) activity, osteocalcin (OCN) production, calcium deposition, and osteogenic gene expression. The alkali-treated NANOZR surface increased ALP activity, OCN production, calcium deposition, and osteogenesis-related gene expression in attached RBM cells. These data suggest that alkali treatment enhances the osteogenesis-inducing capacity of NANOZR implants and may therefore improve their biointegration into alveolar bone.
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Affiliation(s)
- Mariko Nishizaki
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan.
| | - Satoshi Komasa
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan.
| | - Yoichiro Taguchi
- Department of Periodotology, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan.
| | - Hiroshi Nishizaki
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan.
| | - Joji Okazaki
- Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan.
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Civantos A, Martínez-Campos E, Ramos V, Elvira C, Gallardo A, Abarrategi A. Titanium Coatings and Surface Modifications: Toward Clinically Useful Bioactive Implants. ACS Biomater Sci Eng 2017; 3:1245-1261. [DOI: 10.1021/acsbiomaterials.6b00604] [Citation(s) in RCA: 162] [Impact Index Per Article: 20.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/08/2023]
Affiliation(s)
- Ana Civantos
- Tissue
Engineering Group, Institute of Biofunctional Studies, Associated
Unit to the Institute of Polymer Science and Technology (CSIC), Pharmacy
Faculty, Complutense University of Madrid (UCM), Paseo Juan XXIII 1, 28040 Madrid, Spain
- Polymer
Functionalization Group, Institute of Polymer Science and Technology, ICTP-CSIC, Juan de la Cierva, 3, 28006 Madrid, Spain
| | - Enrique Martínez-Campos
- Tissue
Engineering Group, Institute of Biofunctional Studies, Associated
Unit to the Institute of Polymer Science and Technology (CSIC), Pharmacy
Faculty, Complutense University of Madrid (UCM), Paseo Juan XXIII 1, 28040 Madrid, Spain
- Polymer
Functionalization Group, Institute of Polymer Science and Technology, ICTP-CSIC, Juan de la Cierva, 3, 28006 Madrid, Spain
| | - Viviana Ramos
- Tissue
Engineering Group, Institute of Biofunctional Studies, Associated
Unit to the Institute of Polymer Science and Technology (CSIC), Pharmacy
Faculty, Complutense University of Madrid (UCM), Paseo Juan XXIII 1, 28040 Madrid, Spain
- Noricum S.L., San Sebastián
de los Reyes, Av. Fuente Nueva, 14, 28703 Madrid, Spain
| | - Carlos Elvira
- Polymer
Functionalization Group, Institute of Polymer Science and Technology, ICTP-CSIC, Juan de la Cierva, 3, 28006 Madrid, Spain
| | - Alberto Gallardo
- Polymer
Functionalization Group, Institute of Polymer Science and Technology, ICTP-CSIC, Juan de la Cierva, 3, 28006 Madrid, Spain
| | - Ander Abarrategi
- Haematopoietic
Stem Cell Laboratory, The Francis Crick Institute, 1 Midland
Road, NW1 1AT London, U.K
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