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Dong Q, Liang C, Tian C, Ji X, Chang Y, Wang H. Prevalence and genotypic identification of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in farmed ostriches (Struthio camelus) by the Yellow River in Zhengzhou city, central China. BMC Vet Res 2025; 21:82. [PMID: 39985028 PMCID: PMC11846355 DOI: 10.1186/s12917-025-04511-7] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/12/2024] [Accepted: 01/21/2025] [Indexed: 02/23/2025] Open
Abstract
BACKGROUND Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are significant zoonotic protozoa causing gastrointestinal diseases in humans and animals. However, their prevalence and genotypic characterization in ostriches (Struthio camelus) remain poorly understood. This study aimed to investigate the occurrence and genetic diversity of these parasites in farmed ostriches by the Yellow River in Zhengzhou City, central China. A total of 156 fecal samples were collected and analyzed using polymerase chain reaction (PCR) to determine the prevalence and assess the potential epidemiological roles of ostriches in transmission. RESULTS The overall prevalence of Cryptosporidium spp., G. duodenalis and E. bieneusi was 2.56% (4/156), 1.28% (2/156) and 12.2% (19/156), respectively. C. baileyi and G. duodenalis assemblage B were identified in ostriches. Six E. bieneusi genotypes were identified in this study, comprising four previously reported genotypes (EbpA, EbpC, Henan-IV, and Type IV) and two novel genotypes (designated as COW1 and COW2, which differed from known genotypes by two single nucleotide polymorphisms). Among these, EbpC was identified as the predominant genotype. All six genotypes were phylogenetically assigned to zoonotic group 1. CONCLUSIONS Our findings suggest that ostriches harbor zoonotic genotypes of G. duodenalis and E. bieneusi, indicating they may serve as potential reservoirs for human infection. This underscores the need for effective control measures to prevent environmental contamination and reduce the risk of transmission to humans and other animals.
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Affiliation(s)
- Qing Dong
- College of Animal Veterinary Medicine, Henan University of Animal Husbandry and Economy, No. 6 Longzihu University Area, Zhengdong New District, Zhengzhou, Henan, 450046, China
| | - Chaoqun Liang
- Henan Yinji Jiabao Amusement park Management Co., LTD, No. 1 Dahong Road, Liuzhai Town, Xinmi City, Zhengzhou, Henan, 452376, P. R. China
| | - Chao Tian
- Institute of Pet Technology, Henan Vocational College of Agriculture, No. 38 Youth West Road, Zhongmu City, Zhengzhou, Henan, 451450, P. R. China
| | - Xiangbo Ji
- College of Animal Veterinary Medicine, Henan University of Animal Husbandry and Economy, No. 6 Longzihu University Area, Zhengdong New District, Zhengzhou, Henan, 450046, China
| | - Yankai Chang
- College of Animal Veterinary Medicine, Henan University of Animal Husbandry and Economy, No. 6 Longzihu University Area, Zhengdong New District, Zhengzhou, Henan, 450046, China
| | - Haiyan Wang
- College of Animal Veterinary Medicine, Henan University of Animal Husbandry and Economy, No. 6 Longzihu University Area, Zhengdong New District, Zhengzhou, Henan, 450046, China.
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Khordadmehr M, Sazmand A, Oryan A, Almasi P, Ranjbar V, Hemphill A, Otranto D. Respiratory and intestinal zoonotic cryptosporidiosis in symptomatic domestic pigeons ( Columba livia domestica) in Tabriz, Iran. IRANIAN JOURNAL OF VETERINARY RESEARCH 2025; 25:326-332. [PMID: 40386105 PMCID: PMC12085204 DOI: 10.22099/ijvr.2024.49762.7335] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/18/2024] [Accepted: 12/02/2024] [Indexed: 05/20/2025]
Abstract
Background Domestic pigeons (Columba livia domestica) are the oldest domesticated birds worldwide, harboring many zoonotic parasites and posing potential public health threats. Aims To investigate cryptosporidiosis in domestic pigeons in Tabriz, Iran, 100 privately owned pigeons presenting weight loss and diarrhea were tested for Cryptosporidium spp. through parasitological, histopathological, and molecular tests. Methods Modified Ziehl-Neelsen-stained fecal smears and histological sections of the trachea and small intestine were examined microscopically. Genomic DNA of fecal and tracheal specimens was examined by nested conventional PCR targeting 18S rDNA, followed by Sanger sequencing of histopathology-confirmed samples and phylogenetic analyses. Results All pigeons were positive at PCR in their feces and trachea. Oocysts similar to the size of Cryptosporidium species were observed in stained fecal smears of 62% of pigeons. At the histopathological examination, Cryptosporidium-organisms were observed on the apical epithelial surfaces of the small intestine in 84% and trachea in 78% of pigeons. In 23 pigeons, simultaneous tracheal and intestinal cryptosporidiosis was determined. The lesions in affected tracheas and small intestines included hyperemia, villous atrophy and fusion, dilatation of intestinal crypts, irregular epithelial hyperplasia, and sloughing. Diffused mixed inflammatory cell infiltration in the lamina propria was observed, with dominant lymphocytes, plasma cells, and lower numbers of heterophils. Consensus sequences of detected parasites revealed infection with Cryptosporidium parvum and Cryptosporidium meleagridis. Conclusion Considering the high frequency of cryptosporidiosis reported here in symptomatic birds and that both identified Cryptosporidium species are zoonotic parasites, findings claim a public health risk assessment of this species of animals.
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Affiliation(s)
- M. Khordadmehr
- Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
| | - A. Sazmand
- Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran;
| | - A. Oryan
- Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;
| | - P. Almasi
- Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran;
| | - V. Ranjbar
- Ph.D. in Poultry Diseases, Clinic of Poultry Diseases, Yazd Province, Yazd, Iran;
| | - A. Hemphill
- Institute of Parasitology, Vetsuisse Faculty, University of Bern, Länggass-Strasse 122, 3012 Bern, Switzerland
| | - D. Otranto
- Department of Veterinary Medicine, University of Bari, Valenzano, Bari 70010, Italy
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Essam A, Elmishmishy B, Hammad E, Elwafa SA, Abbas I. Occurrence and molecular characterization of Cryptosporidium oocysts in chickens from Egypt, and a meta-analysis for Cryptosporidium infections in chickens worldwide. Vet Parasitol Reg Stud Reports 2025; 57:101169. [PMID: 39855857 DOI: 10.1016/j.vprsr.2024.101169] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/28/2024] [Revised: 10/30/2024] [Accepted: 11/14/2024] [Indexed: 01/27/2025]
Abstract
Cryptosporidium is a leading cause of diarrhea in children and immunocompromised patients. Various animals and birds can also be infected with this protist, and Cryptosporidium zoonosis is common. A few reports have been published worldwide on Cryptosporidium infections in chickens. The present study surveyed Cryptosporidium infections in chickens from Dakahlia governorate, Egypt. Cryptosporidium oocysts were tested, using the Ziehl Neelsen modified acid-fast staining, in 884 chickens collected from various farms and live bird markets (LBMs) in Dakahlia governorate, Egypt. COWP (Cryptosporidium oocyst wall protein)-PCR testing coupled with isolate sequencing was conducted for 10 Cryptosporidium-positive samples. Intestinal contents of 58 (6.6 %) out of the surveyed chickens in Dakahlia, Egypt, had Cryptosporidium oocysts. The native breed (Balady) had a significantly higher prevalence than the commercial breeds. Oocysts of two different size ranges were detected; 4-5.5 × 4-5 μm and 6-7 × 5-6 μm. Two of the ten PCR-tested samples were successfully sequenced and identified as Cryptosporidium parvum. The present study conducted also the first meta-analysis describing the epidemiology, clinical disease, and species diversity of Cryptosporidium infecting chickens worldwide. The pooled Cryptosporidium prevalence in 18,579 chickens tested worldwide was 18.3 %, with insignificant geographical variations. The prevalence was two-fold higher (40.5 %) at the flock level. Chickens collected from LBMs (30.5 %) had significantly higher pooled prevalence than farmed chickens (14.4 %). Insignificant prevalence variations were detected according to the production type (broiler or layer), diagnostic method, or disease status. Three Cryptosporidium species common to infect birds were molecularly identified in 760 chickens tested worldwide: Cryptosporidium baileyi, Cryptosporidium meleagridis, and Cryptosporidium galli, with the former being the most frequently detected. Cryptosporidium parvum was detected in a few samples mostly from free-range chickens. In conclusion, potential C. parvum zoonosis from chickens is suggested in Egypt, where C. parvum is common among humans and various animals. Given that chicken has a substantial role in the global C. meleagridis zoonoses, and no bird-specific Cryptosporidium spp. were detected in the tested samples from Egypt, we do recommend a large-scale molecular survey to detect various species and genotypes infecting chickens throughout various Egyptian governorates.
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Affiliation(s)
- Ahmed Essam
- Parasitology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
| | - Bassem Elmishmishy
- Parasitology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
| | - Enas Hammad
- Department of Poultry Diseases, Animal Health Research Institute, Mansoura Branch, Agricultural Research Center (ARC), Egypt
| | - Salah Abu Elwafa
- Parasitology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
| | - Ibrahim Abbas
- Parasitology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
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Gomes-Gonçalves S, Rodrigues D, Santos N, Gantois N, Chabé M, Viscogliosi E, Mesquita JR. Molecular Screening and Characterization of Enteric Protozoan Parasites and Microsporidia in Wild Ducks from Portugal. Animals (Basel) 2024; 14:2956. [PMID: 39457886 PMCID: PMC11503927 DOI: 10.3390/ani14202956] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/27/2024] [Revised: 10/11/2024] [Accepted: 10/12/2024] [Indexed: 10/28/2024] Open
Abstract
Enteric parasites pose significant threats to both human and veterinary health, ranking among the top causes of mortality worldwide. Wild migratory waterfowl, such as ducks, may serve as hosts and vectors for these parasites, facilitating their transmission across ecosystems. This study conducted a molecular screening of enteric parasites in three species of wild ducks of the genus Anas (A. acuta, A. platyrhynchos and A. crecca) from Portugal, targeting Blastocystis sp., Balantioides coli, Cryptosporidium spp., Encephalitozoon spp., and Enterocytozoon bieneusi. Fecal samples from 71 ducks were analyzed using PCR and sequencing techniques. The results revealed a 2.82% occurrence of Blastocystis sp. subtype 7 and Cryptosporidium baileyi, marking the first molecular detection of these pathogens in wild ducks in Portugal. While previous studies have documented these parasites in Anas spp. in other regions, this study contributes novel data specific to the Portuguese context. No evidence of Balantioides coli, Encephalitozoon spp. or Enterocytozoon bieneusi was found. These findings highlight the potential role of migratory ducks as vectors for zoonotic protozoa, emphasizing the need for enhanced surveillance of avian populations to mitigate cross-species transmission risks. Further research is warranted to understand the global public health implications associated with migratory waterfowl.
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Affiliation(s)
- Sara Gomes-Gonçalves
- School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal
| | - David Rodrigues
- Coimbra College of Agriculture, Polytechnic University of Coimbra, 3045-601 Coimbra, Portugal
- Forest Research Centre, School of Agriculture, University of Lisbon, 1649-004 Lisboa, Portugal
| | - Nuno Santos
- Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Universidade do Porto, 4485-661 Vairão, Portugal
| | - Nausicaa Gantois
- CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019–UMR 9017–CIIL–Centre d’Infection et d’Immunité de Lille, Université Lille, F-59000Lille, France (M.C.); (E.V.)
| | - Magali Chabé
- CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019–UMR 9017–CIIL–Centre d’Infection et d’Immunité de Lille, Université Lille, F-59000Lille, France (M.C.); (E.V.)
| | - Eric Viscogliosi
- CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019–UMR 9017–CIIL–Centre d’Infection et d’Immunité de Lille, Université Lille, F-59000Lille, France (M.C.); (E.V.)
| | - João R. Mesquita
- School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal
- Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto, 4050-600 Porto, Portugal
- Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR), 4050-600 Porto, Portugal
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Egan S, Barbosa AD, Feng Y, Xiao L, Ryan U. The risk of wild birds contaminating source water with zoonotic Cryptosporidium and Giardia is probably overestimated. THE SCIENCE OF THE TOTAL ENVIRONMENT 2024; 912:169032. [PMID: 38123098 DOI: 10.1016/j.scitotenv.2023.169032] [Citation(s) in RCA: 4] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/27/2023] [Revised: 11/29/2023] [Accepted: 11/29/2023] [Indexed: 12/23/2023]
Abstract
Cryptosporidium and Giardia are important waterborne protozoan parasites that are resistant to disinfectants commonly used for drinking water. Wild birds, especially wild migratory birds, are often implicated in the contamination of source and wastewater with zoonotic diseases, due to their abundance near water and in urban areas and their ability to spread enteric pathogens over long distances. This review summarises the diversity of Cryptosporidium and Giardia in birds, with a focus on zoonotic species, particularly in wild and migratory birds, which is critical for understanding zoonotic risks. The analysis revealed that both avian-adapted and zoonotic Cryptosporidium species have been identified in birds but that avian-adapted Cryptosporidium species dominate in wild migratory birds. Few studies have examined Giardia species and assemblages in birds, but the non-zoonotic Giardia psittaci and Giardia ardeae are the most commonly reported species. The identification of zoonotic Cryptosporidium and Giardia in birds, particularly C. parvum and G. duodenalis assemblages A and B in wild migratory birds, is likely due to mechanical carriage or spillback from birds co-grazing pastures contaminated with C. parvum from livestock. Therefore, the role of wild migratory birds in the transmission of zoonotic Cryptosporidium and Giardia to source water is likely overestimated. To address knowledge gaps, it is important to conduct more extensive studies on the prevalence of Cryptosporidium and Giardia in a broader range of migratory wild birds. There is also a need to investigate the extent to which zoonotic infections with C. hominis/C. parvum and G. duodenalis assemblages A and B are mechanical and/or transient, and to assess the load and viability of zoonotic oo/cysts shed in avian faeces. Understanding the contribution of birds to zoonoses is essential for effective disease surveillance, prevention, and control.
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Affiliation(s)
- Siobhon Egan
- Harry Butler Institute, Vector- and Water-Borne Pathogen Research Group, Murdoch University, Murdoch, Western Australia 6150, Australia.
| | - Amanda D Barbosa
- Harry Butler Institute, Vector- and Water-Borne Pathogen Research Group, Murdoch University, Murdoch, Western Australia 6150, Australia; CAPES Foundation, Ministry of Education of Brazil, Brasilia, DF 70040-020, Brazil
| | - Yaoyu Feng
- Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
| | - Lihua Xiao
- Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
| | - Una Ryan
- Harry Butler Institute, Vector- and Water-Borne Pathogen Research Group, Murdoch University, Murdoch, Western Australia 6150, Australia
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Pane S, Putignani L. Cryptosporidium: Still Open Scenarios. Pathogens 2022; 11:pathogens11050515. [PMID: 35631036 PMCID: PMC9143492 DOI: 10.3390/pathogens11050515] [Citation(s) in RCA: 15] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/08/2022] [Revised: 04/20/2022] [Accepted: 04/23/2022] [Indexed: 01/27/2023] Open
Abstract
Cryptosporidiosis is increasingly identified as a leading cause of childhood diarrhea and malnutrition in both low-income and high-income countries. The strong impact on public health in epidemic scenarios makes it increasingly essential to identify the sources of infection and understand the transmission routes in order to apply the right prevention or treatment protocols. The objective of this literature review was to present an overview of the current state of human cryptosporidiosis, reviewing risk factors, discussing advances in the drug treatment and epidemiology, and emphasizing the need to identify a government system for reporting diagnosed cases, hitherto undervalued.
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Affiliation(s)
- Stefania Pane
- Department of Diagnostic and Laboratory Medicine, Bambino Gesù Children’s Hospital, IRCCS, Unit of Microbiology and Diagnostic Immunology, Unit of Microbiomics, 00146 Rome, Italy;
| | - Lorenza Putignani
- Department of Diagnostic and Laboratory Medicine, Bambino Gesù Children’s Hospital, IRCCS, Unit of Microbiology and Diagnostic Immunology, Unit of Microbiomics and Multimodal Laboratory Medicine Research Area, Unit of Human Microbiome, 00146 Rome, Italy
- Correspondence:
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Feng X, Xin L, Yu F, Song X, Zhang J, Deng J, Qi M, Zhao W. Genetic characterization of Cryptosporidium spp. in Hotan Black Chickens in China reveals two novel subtypes of Cryptosporidium meleagridis. Parasite 2022; 29:50. [PMCID: PMC9645226 DOI: 10.1051/parasite/2022051] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/15/2022] [Accepted: 10/06/2022] [Indexed: 11/11/2022] Open
Abstract
A total of 617 fecal specimens were collected on 18 Hotan Black chicken farms in Southern Xinjiang, China, and tested for the presence of Cryptosporidium spp. by PCR of the small subunit ribosomal RNA (SSU rRNA) gene. The overall infection rate by Cryptosporidium spp. was 11.5% (71/617), and ten of the 18 farms were positive. The infection rate by Cryptosporidium spp. was 14.5% (48/331) in the 30–60 d group, higher than chickens in the <30 d (12.0%, 15/125), 60–90 d (6.9%, 5/72), and >90 d (3.4%, 3/89) groups. Cryptosporidium meleagridis (n = 38) and C. baileyi (n = 33) were confirmed by sequencing analysis. A total of 25 of the 38 C. meleagridis-positive specimens were subtyped successfully at the gp60 gene, including one known subtype (IIIbA23G1R1, n = 1) and two novel subtypes, named IIIbA25G1R1 (n = 20) and IIIbA31G1R1 (n = 4). The results showed that infection by Cryptosporidium spp. in Hotan Black Chickens was common in this area and the distribution of C. meleagridis subtypes had regional characteristics.
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Affiliation(s)
- Xinwei Feng
- College of Animal Science and Technology, Tarim University Alar Xinjiang 843300 China
| | - Luyao Xin
- College of Animal Science and Technology, Tarim University Alar Xinjiang 843300 China
| | - Fuchang Yu
- College of Animal Science and Technology, Tarim University Alar Xinjiang 843300 China
| | - Xianming Song
- Xinjiang Agricultural Vocational Technical College Changji Xinjiang 831199 China
| | - Jianing Zhang
- College of Animal Science and Technology, Tarim University Alar Xinjiang 843300 China
| | - Jinhua Deng
- College of Animal Science and Technology, Tarim University Alar Xinjiang 843300 China
| | - Meng Qi
- College of Animal Science and Technology, Tarim University Alar Xinjiang 843300 China
- Corresponding authors: ;
| | - Wei Zhao
- Department of Parasitology, Wenzhou Medical University Wenzhou Zhejiang 325035 China
- Corresponding authors: ;
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Prasad N, Bansal S, Akhtar S. Cryptosporidium infection in solid organ transplant recipients in South Asia - Expert group opinion for diagnosis and management. INDIAN JOURNAL OF TRANSPLANTATION 2022. [DOI: 10.4103/ijot.ijot_80_21] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/06/2022] Open
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Wu XM, Yang X, Fan XC, Chen X, Wang YX, Zhang LX, Song JK, Zhao GH. Serum metabolomics in chickens infected with Cryptosporidium baileyi. Parasit Vectors 2021; 14:336. [PMID: 34174965 PMCID: PMC8235856 DOI: 10.1186/s13071-021-04834-y] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/09/2021] [Accepted: 06/09/2021] [Indexed: 11/13/2022] Open
Abstract
Background Cryptosporidium baileyi is an economically important zoonotic pathogen that causes serious respiratory symptoms in chickens for which no effective control measures are currently available. An accumulating body of evidence indicates the potential and usefulness of metabolomics to further our understanding of the interaction between pathogens and hosts, and to search for new diagnostic or pharmacological biomarkers of complex microorganisms. The aim of this study was to identify the impact of C. baileyi infection on the serum metabolism of chickens and to assess several metabolites as potential diagnostic biomarkers for C. baileyi infection. Methods Ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) and subsequent multivariate statistical analysis were applied to investigate metabolomics profiles in the serum samples of chickens infected with C. baileyi, and to identify potential metabolites that can be used to distinguish chickens infected with C. baileyi from non-infected birds. Results Multivariate statistical analysis identified 138 differential serum metabolites between mock- and C. baileyi-infected chickens at 5 days post-infection (dpi), including 115 upregulated and 23 downregulated compounds. These metabolites were significantly enriched into six pathways, of which two pathways associated with energy and lipid metabolism, namely glycerophospholipid metabolism and sphingolipid metabolism, respectively, were the most enriched. Interestingly, some important immune-related pathways were also significantly enriched, including the intestinal immune network for IgA production, autophagy and cellular senescence. Nine potential C. baileyi-responsive metabolites were identified, including choline, sirolimus, all-trans retinoic acid, PC(14:0/22:1(13Z)), PC(15:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)), PE(16:1(9Z)/24:1(15Z)), phosphocholine, SM(d18:0/16:1(9Z)(OH)) and sphinganine. Conclusions This is the first report on serum metabolic profiling of chickens with early-stage C. baileyi infection. The results provide novel insights into the pathophysiological mechanisms of C. baileyi in chickens. Graphic abstract ![]()
Supplementary Information The online version contains supplementary material available at 10.1186/s13071-021-04834-y.
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Affiliation(s)
- Xue-Mei Wu
- Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, People's Republic of China
| | - Xin Yang
- Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, People's Republic of China
| | - Xian-Cheng Fan
- Center of Animal Disease Prevention and Control of Huyi District, Xi'an, 710300, People's Republic of China
| | - Xi Chen
- Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, People's Republic of China
| | - Yu-Xin Wang
- Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, People's Republic of China
| | - Long-Xian Zhang
- College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, People's Republic of China
| | - Jun-Ke Song
- Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, People's Republic of China
| | - Guang-Hui Zhao
- Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, People's Republic of China.
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Kopacz Ż, Kváč M, Piesiak P, Szydłowicz M, Hendrich AB, Sak B, McEvoy J, Kicia M. Cryptosporidium baileyi Pulmonary Infection in Immunocompetent Woman with Benign Neoplasm. Emerg Infect Dis 2021; 26:1958-1961. [PMID: 32687044 PMCID: PMC7392468 DOI: 10.3201/eid2608.201117] [Citation(s) in RCA: 17] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/19/2022] Open
Abstract
Cryptosporidium baileyi, a bird-specific parasite, infects gastrointestinal, pulmonary, and urinary tracts of its host. We report on a C. baileyi infection associated with pulmonary hamartoma in an immunocompetent patient in Poland. Further work is needed to investigate the association between Cryptosporidium infections and tumors.
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Shahbazi P, Aligolzadeh A, Khordadmehr M, Hashemzadeh Farhang H, Katiraee F. Molecular study and genotyping of Cryptosporidium baileyi and Cryptosporidium parvum from free-range and commercial broiler chickens in Guilan province, Iran. Comp Immunol Microbiol Infect Dis 2020; 69:101411. [PMID: 31951878 DOI: 10.1016/j.cimid.2019.101411] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/03/2019] [Revised: 12/23/2019] [Accepted: 12/24/2019] [Indexed: 11/18/2022]
Abstract
Cryptosporidiosis acutely impacts the digestive and/or respiratory tract of the birds in many species of various orders. More importantly, it is also well known as a significant zoonotic disease, which can lead to diarrhea in humans and livestock. Regarding increasing demand for free-range products and increasing the number of free-range poultry farms, the present paper evaluated histopathological and molecular detection of Cryptosporidium baileyi and Cryptosporidium parvum in free-range and commercial broiler chickens in the north part of Iran. For this purpose, 100 fecal and tissue samples of the chickens in Guilan province were collected. After microscopic examination using Ziehl-Neelsen staining, molecular analyses of the fecal samples were processed by Nested-PCR targeting the 18S rRNA gene followed by sequencing of the amplicons and phylogenetic analyses. Eventually, the tissue samples were studied for histological lesions. Findings demonstrated the presence of Cryptosporidium baileyi and Cryptosporidium parvum in 6 % and 2 % of fecal samples, respectively. This is the first identification of C.parvum in avian hosts in Iran, and for the first time, C.baileyi and C.parvum are shown in native free-range chickens in Iran. All of the PCR positive birds with clinical symptoms showed gross lesions of respiratory infections. There was no significant difference between infection rate in free-range and commercial broiler chickens; however, the infection rate was significantly higher in chickens <25 days old. To conclude, we present here a notable Cryptosporidium infection rate in the free-range chicks in Iran, which notify the role of this host as a reservoir and should be more noted due to the economic and zoonotic importance.
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Affiliation(s)
- Parisa Shahbazi
- Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
| | - Armin Aligolzadeh
- Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
| | - Monireh Khordadmehr
- Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
| | - Hosein Hashemzadeh Farhang
- Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
| | - Farzad Katiraee
- Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
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Zhao W, Zhou HH, Ma TM, Cao J, Lu G, Shen YJ. PCR-Based Detection of Cryptosporidium spp. and Enterocytozoon bieneusi in Farm-Raised and Free-Ranging Geese ( Anser anser f. domestica) From Hainan Province of China: Natural Infection Rate and the Species or Genotype Distribution. Front Cell Infect Microbiol 2019; 9:416. [PMID: 31867290 PMCID: PMC6904268 DOI: 10.3389/fcimb.2019.00416] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/27/2019] [Accepted: 11/21/2019] [Indexed: 02/05/2023] Open
Abstract
Cryptosporidium spp. and Enterocytozoon bieneusi are two important zoonotic pathogens that can infect humans and a broad range of animal hosts. However, few studies have been conducted to study infection of the two pathogens in domestic geese until now. The aims of the present study were to determine the prevalence of natural infection, and the species or genotype distribution of Cryptosporidium and E. bieneusi in farm-raised and free-ranging geese from Hainan Province of China. In total, 266 fecal samples of geese were collected (142 farm-raised and 124 free-ranging geese). Cryptosporidium spp. and E. bieneusi were identified by nested PCR and sequencing analysis of the SSU rRNA and the ITS region of the rRNA genes. A total of 4.1% (12/226) of the geese were positive for Cryptosporidium spp., with 0.7% identified in the farm-raised geese and 7.0% in the free-ranging geese. Two bird-adapted species/genotypes were identified: C. baileyi (n = 1) and Cryptosporidium goose genotype I (n = 11). Meanwhile, E. bieneusi was found in 13.9% (37/266) of geese, with 8.9% identified in the farm-raised and 21.8% in the free-ranging geese. Eleven genotypes of E. bieneusi were identified constituted with six known genotypes: D (n = 13), I (n = 5), CHG2 (n = 1), CHG3 (n = 5), and CHG5 (n = 1), and five novel genotypes named HNE-I to V (one each). All of the genotypes identified in the geese here belonged to zoonotic Groups 1 or 2. This study is the first to demonstrate the presence of Cryptosporidium spp. and E. bieneusi in domestic geese from Hainan, China, and provides baseline data that will be useful for controlling and preventing these pathogens in goose farms. The geese infected with E. bieneusi, but not with Cryptosporidium, should be considered potential public health threats.
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Affiliation(s)
- Wei Zhao
- National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
- Chinese Center for Tropical Diseases Research, Shanghai, China
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China
- National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
- Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Department of Parasitology, Wenzhou Medical University, Wenzhou, China
| | - Huan-huan Zhou
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
| | - Tian-ming Ma
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
| | - Jianping Cao
- National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
- Chinese Center for Tropical Diseases Research, Shanghai, China
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China
- National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
- Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
| | - Gang Lu
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
| | - Yu-juan Shen
- National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
- Chinese Center for Tropical Diseases Research, Shanghai, China
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China
- National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
- Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
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Plutzer J, Lassen B, Jokelainen P, Djurković-Djaković O, Kucsera I, Dorbek-Kolin E, Šoba B, Sréter T, Imre K, Omeragić J, Nikolić A, Bobić B, Živičnjak T, Lučinger S, Stefanović LL, Kučinar J, Sroka J, Deksne G, Keidāne D, Kváč M, Hůzová Z, Karanis P. Review of Cryptosporidium and Giardia in the eastern part of Europe, 2016. ACTA ACUST UNITED AC 2019; 23. [PMID: 29382412 PMCID: PMC5801338 DOI: 10.2807/1560-7917.es.2018.23.4.16-00825] [Citation(s) in RCA: 35] [Impact Index Per Article: 5.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/03/2022]
Abstract
This paper reviews the current knowledge and understanding of Cryptosporidium spp. and Giardia spp. in humans, animals and the environment in 10 countries in the eastern part of Europe: Bosnia and Herzegovina, Croatia, Czech Republic, Estonia, Hungary, Latvia, Poland, Romania, Serbia and Slovenia. Methods: Published scientific papers and conference proceedings from the international and local literature, official national health service reports, national databases and doctoral theses in local languages were reviewed to provide an extensive overview on the epidemiology, diagnostics and research on these pathogens, as well as analyse knowledge gaps and areas for further research. Results: Cryptosporidium spp. and Giardia spp. were found to be common in eastern Europe, but the results from different countries are difficult to compare because of variations in reporting practices and detection methodologies used. Conclusion: Upgrading and making the diagnosis/detection procedures more uniform is recommended throughout the region. Public health authorities should actively work towards increasing reporting and standardising reporting practices as these prerequisites for the reported data to be valid and therefore necessary for appropriate control plans.
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Affiliation(s)
- Judit Plutzer
- Department of Water Hygiene, National Public Health Institute, Budapest, Hungary
| | - Brian Lassen
- Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark.,Department of Basic Veterinary Sciences and Population Medicine, Institute of Veterinary Medicine and Animal Science, Estonian University of Life Sciences, Tartu, Estonia
| | - Pikka Jokelainen
- Department of Bacteria, Parasites & Fungi, Infectious Disease Preparedness, Statens Serum Institut, Copenhagen, Denmark.,Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.,Department of Basic Veterinary Sciences and Population Medicine, Institute of Veterinary Medicine and Animal Science, Estonian University of Life Sciences, Tartu, Estonia
| | - Olgica Djurković-Djaković
- Centre of Excellence for Food- and Vector-borne Zoonoses, Institute for Medical Research, University of Belgrade, Belgrade, Serbia
| | - István Kucsera
- Department of Parasitology, National Public Health Institute, Budapest, Hungary
| | - Elisabeth Dorbek-Kolin
- Department of Basic Veterinary Sciences and Population Medicine, Institute of Veterinary Medicine and Animal Science, Estonian University of Life Sciences, Tartu, Estonia
| | - Barbara Šoba
- Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
| | - Tamás Sréter
- National Food Chain Safety Office, Veterinary Diagnostic Directorate, Budapest, Hungary
| | - Kálmán Imre
- Banat's University of Agricultural Sciences and Veterinary Medicine 'King Michael I of Romania' from Timişoara, Faculty of Veterinary Medicine, Department of Animal Production and Veterinary Public Health, Timişoara, Romania
| | - Jasmin Omeragić
- University of Sarajevo, Veterinary Faculty, Department of Parasitology and Invasive Diseases of Animals, Sarajevo, Bosnia and Herzegovina
| | - Aleksandra Nikolić
- Centre of Excellence for Food- and Vector-borne Zoonoses, Institute for Medical Research, University of Belgrade, Belgrade, Serbia
| | - Branko Bobić
- Centre of Excellence for Food- and Vector-borne Zoonoses, Institute for Medical Research, University of Belgrade, Belgrade, Serbia
| | - Tatjana Živičnjak
- Department for Parasitology and Parasitic Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
| | - Snježana Lučinger
- Department for Parasitology and Parasitic Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
| | | | - Jasmina Kučinar
- Department of Microbiology, Public Health Institute of Istrian Region, Pula, Croatia
| | - Jacek Sroka
- Department of Parasitology, National Veterinary Research Institute, Puławy, Poland
| | - Gunita Deksne
- Institute of Food Safety, Animal Health and Environment - 'BIOR', Riga, Latvia
| | - Dace Keidāne
- Faculty of Veterinary Medicine, Latvia University of Agriculture, Jelgava, Latvia
| | - Martin Kváč
- Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.,Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic
| | - Zuzana Hůzová
- Health Institute in Ústí nad Labem, Prague, Czech Republic
| | - Panagiotis Karanis
- Medical School, University of Cologne, Cologne, Germany.,State Key Laboratory for Plateau Ecology and Agriculture, Centre for Biomedicine and Infectious Diseases Qinghai University, Xining, China
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Liao C, Wang T, Koehler AV, Fan Y, Hu M, Gasser RB. Molecular investigation of Cryptosporidium in farmed chickens in Hubei Province, China, identifies 'zoonotic' subtypes of C. meleagridis. Parasit Vectors 2018; 11:484. [PMID: 30157928 PMCID: PMC6114272 DOI: 10.1186/s13071-018-3056-5] [Citation(s) in RCA: 32] [Impact Index Per Article: 4.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/20/2018] [Accepted: 08/10/2018] [Indexed: 01/09/2023] Open
Abstract
BACKGROUND Cryptosporidium is a key genus of parasitic protists that infect humans and other vertebrates (mammals and birds). Birds are typically infected with C. avium, C. baileyi, C. galli and/or C. meleagridis, the latter of which is recognised as being zoonotic. Stimulated by the previous finding of C. meleagridis subtypes IIIbA21G1R1, IIIbA22G1R1 and IIIbA26G1R1 in diarrhoeic children in Wuhan city and environs in Hubei Province, China, we performed a molecular epidemiological survey to explore whether these or similar subtypes might occur in farmed chickens in this province. METHODS PCR-coupled sequencing analyses of regions in the small subunit (SSU) of the nuclear ribosomal RNA and 60 kDa glycoprotein (gp60) genes were utilised to characterise Cryptosporidium in faecal samples from chickens (n = 471) from 14 farms from six distinct regions in Hubei Province. RESULTS Cryptosporidium baileyi (33/471; 7.0%) and C. meleagridis (15/471; 3.2%) were identified in chickens on eight farms in five of the six distinct geographical regions. No significant age-associated difference in the prevalence of C. baileyi was evident, whereas the prevalence of C. meleagridis was significantly higher in younger (≤ 4 months) than in older chickens (> 4 months). For C. meleagridis, two subtype families, IIIb and IIIe, were defined; some of the subtypes (i.e. IIIbA26G1R1b and IIIbA22G1R1c) characterised here matched those identified previously in diarrhoeic children in Wuhan. CONCLUSIONS This is the first molecular study reporting the genetic identity and prevalence of C. baileyi and C. meleagridis in chickens in Hubei. The findings suggest that C. meleagridis subtypes IIIbA26G1R1b and IIIbA22G1R1c are cross-transmissible between chickens and humans, raising awareness about the significance of birds as potential reservoirs of zoonotic variants of Cryptosporidium. Future studies might focus on investigating the prevalence of 'zoonotic' subtypes of Cryptosporidium meleagridis in various species of wild and domesticated birds, and on comparing them with those found in humans in China and other countries.
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Affiliation(s)
- Cong Liao
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China
| | - Tao Wang
- Department of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Parkville, Victoria, Australia.
| | - Anson V Koehler
- Department of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Parkville, Victoria, Australia
| | - Yingying Fan
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China
| | - Min Hu
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China
| | - Robin B Gasser
- State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. .,Department of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Parkville, Victoria, Australia.
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Ren GJ, Fan XC, Liu TL, Wang SS, Zhao GH. Genome-wide analysis of differentially expressed profiles of mRNAs, lncRNAs and circRNAs during Cryptosporidium baileyi infection. BMC Genomics 2018; 19:356. [PMID: 29747577 PMCID: PMC5946474 DOI: 10.1186/s12864-018-4754-2] [Citation(s) in RCA: 24] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/18/2017] [Accepted: 05/02/2018] [Indexed: 01/17/2023] Open
Abstract
Background Cryptosporidium baileyi is the most common Cryptosporidium species in birds. However, effective prevention measures and treatment for C. baileyi infection were still not available. Long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) play important roles in regulating occurrence and progression of many diseases and are identified as effective biomarkers for diagnosis and prognosis of several diseases. In the present study, the expression profiles of host mRNAs, lncRNAs and circRNAs associated with C. baileyi infection were investigated for the first time. Results The tracheal tissues of experimental (C. baileyi infection) and control chickens were collected for deep RNA sequencing, and 545,479,934 clean reads were obtained. Of them, 1376 novel lncRNAs were identified, including 1161 long intergenic non-coding RNAs (lincRNAs) and 215 anti-sense lncRNAs. A total of 124 lncRNAs were found to be significantly differentially expressed between the experimental and control groups. Additionally, 14,698 mRNAs and 9085 circRNAs were identified, and significantly different expressions were observed for 1317 mRNAs and 104 circRNAs between two groups. Bioinformatic analyses of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway for their targets and source genes suggested that these dysregulated genes may be involved in the interaction between the host and C. baileyi. Conclusions The present study revealed the expression profiles of mRNAs, lncRNAs and circRNAs during C. baileyi infection for the first time, and sheds lights on the roles of lncRNAs and circRNAs underlying the pathogenesis of Cryptosporidium infection. Electronic supplementary material The online version of this article (10.1186/s12864-018-4754-2) contains supplementary material, which is available to authorized users.
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Affiliation(s)
- Guan-Jing Ren
- Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China
| | - Xian-Cheng Fan
- Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China
| | - Ting-Li Liu
- Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China
| | - Sha-Sha Wang
- Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China
| | - Guang-Hui Zhao
- Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China.
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Bautista M, Bonatti TR, Fiuza VRDS, Terashima A, Canales-Ramos M, José J, Franco RMB. Occurrence and molecular characterization of Giardia duodenalis cysts and Cryptosporidium oocysts in raw water samples from the Rímac River, Peru. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2018; 25:11454-11467. [PMID: 29423699 DOI: 10.1007/s11356-018-1423-6] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/07/2017] [Accepted: 01/29/2018] [Indexed: 06/08/2023]
Abstract
Giardia and Cryptosporidium are potentially pathogenic protozoa which are ubiquitous in ambient surface water. The present study included 60 samples of surface water from three sampling sites from the Rímac River, Lima and Callao, Peru, to detect the occurrence of Giardia spp. and Cryptosporidium spp. and to perform molecular characterization of specimens found. Water samples were concentrated using the membrane filtration technique, and following elution, cysts and oocysts were visualized by direct immunofluorescence assay (IFA). For molecular characterization, tpi and bg gene fragments and 18S rRNA were amplified by nested PCR for Giardia and Cryptosporidium, respectively, followed by sequencing and phylogenetic analysis. Giardia cysts were found in 93.3% of the analyzed samples, whereas Cryptosporidium oocysts were detected in 15%. The positivity of the Giardia cysts was 86.6% (n = 26) in 2014, while Cryptosporidium oocysts were not detected. In 2015, both protozoa were found in raw water samples, with all 30 samples collected positive for Giardia cysts (100.0%) and 9 positive for Cryptosporidium oocysts (30.0%). Oocysts were detected in 20.0% of water samples from sites 1 (mean 5.25 oocysts/L) and 2 (mean 52.3 oocysts/L), while at site 3, oocysts were detected in 50.0% of raw water samples (mean 193.6 oocysts/L). The presence of Giardia duodenalis assemblage A was confirmed in several samples by the phylogenetic positioning of the bg and tpi genes, and the sub-assemblage AII was predominant (8/9). Sequencing for Cryptosporidium resulted in profiles compatible with Cryptosporidium hominis, Cryptosporidium meleagridis, and Cryptosporidium baileyi. This is the first time that the presence of G. duodenalis assemblage A/sub-assemblage AII and Cryptosporidium species has been reported in surface water samples in Peru. These Cryptosporidium species and the Giardia duodenalis assemblage are associated with human disease which highlights the potential risk to public health and the need to increase environmental monitoring measures to protect this water body.
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Affiliation(s)
- Meylin Bautista
- Post-Graduate Program in Animal Biology, Biology Institute, UNICAMP, Campinas, SP, Brazil
| | - Taís Rondello Bonatti
- Post-Graduate Program in Animal Biology, Biology Institute, UNICAMP, Campinas, SP, Brazil
| | - Vagner Ricardo da S Fiuza
- Oxidative Processes Laboratory, School of Civil Engineering, Architecture and Urban Design, UNICAMP, Campinas, SP, Brazil
| | - Angelica Terashima
- Parasitology Laboratory, Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru
| | - Marco Canales-Ramos
- Parasitology Laboratory, Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru
| | - Juliana José
- Laboratory of Genomics and Expression (LGE), Genetics, Evolution, Microbiology and Immunology Department, Biology Institute, UNICAMP, Campinas, SP, Brazil
| | - Regina Maura Bueno Franco
- Protozoology Laboratory, Animal Biology Department, Biology Institute, Universidade Estadual de Campinas, UNICAMP, Rua Monteiro Lobato, n° 255, Campinas, SP, Brazil.
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Briceño C, Surot D, González-Acuña D, Martínez FJ, Fredes F. Parasitic survey on introduced monk parakeets (Myiopsitta monachus) in Santiago, Chile. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA 2017; 26:129-135. [DOI: 10.1590/s1984-29612017023] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/14/2016] [Accepted: 03/31/2017] [Indexed: 11/22/2022]
Abstract
Abstract Central Chile has been identified as a unique ecosystem with high conservation priority because of its high levels of endemism and intensive anthropic pressure. Over a period of almost four decades, the monk parakeet has been successful in establishing and dispersing in urban Santiago, although little is known about its potential impact. Furthermore, nothing is known about its epidemiological risks towards animals or even humans. For this reason, we conducted the first parasitic survey of monk parakeets in Chile through capture, necropsy and thorough external and internal inspection of 92 adult individuals. Among these, 45.7% presented lice that were identified as Paragoniocotes fulvofasciatum, 1.1% had mesostigmatid acari and 8.9% had free-ranging acari. Among 89 parakeets, 19.1% had structures identified as Cryptosporidium sp. This study provides the first description of Cryptosporidium sp. in monk parakeets. Along with the presence of a mesostigmatid acarus in one parakeet, this serves as a public health warning, given that both of these parasites have zoonotic potential.
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Nakagun S, Horiuchi N, Sugimoto M, Tomikawa S, Watanabe K, Kobayashi Y. Proventriculitis Associated with Cryptosporidium baileyi in a Snowy Owl (Bubo scandiacus) and Its Epidemiological Investigation. J Parasitol 2017; 103:451-457. [PMID: 28528556 DOI: 10.1645/17-54] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/10/2022] Open
Abstract
We describe an unusual case of proventriculitis associated with Cryptosporidium baileyi in a 7-wk-old snowy owl (Bubo scandiacus) chick kept at a zoo. Necropsy of this animal revealed diffuse mucosal thickening of the proventriculus. Subsequent histopathological examinations of the proventriculus showed marked ductal epithelial hyperplasia with intestinal metaplasia and severe inflammatory cell infiltration in the lamina propria and submucosa. These lesions were associated with numerous periodic-acid-Schiff-positive cryptosporidia-like protozoan parasites. Moreover, oocysts found within the lamina propria had a noticeably thicker wall and displayed Ziehl-Neelsen-positive test results. PCR sequencing analyses of the 18S rDNA, actin, and 70 kDa heat shock protein gene loci identified the protozoan to be C. baileyi, of which two novel sets of primers were designed for use with formalin-fixed paraffin-embedded tissue. An epidemiological survey was carried out at the zoo to investigate the source of infection, but all owl species surveyed proved negative for cryptosporidiosis. It is most likely that small animal vectors such as wild birds or rodents were responsible for this particular lethal case. This is the first report of C. baileyi associated with proventriculitis and also the first report of cryptosporidiosis in a raptor species in Asia.
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Affiliation(s)
- Shotaro Nakagun
- Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
| | - Noriyuki Horiuchi
- Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
| | - Miki Sugimoto
- Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
| | - Sohei Tomikawa
- Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
| | - Kenichi Watanabe
- Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
| | - Yoshiyasu Kobayashi
- Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
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Alves M, Matos O, Spano F, Antunes F. PCR-RFLP analysis ofCryptosporidium parvumisolates from HIV-infected patients in Lisbon, Portugal. ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY 2016. [DOI: 10.1080/00034983.2000.11813541] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/28/2022]
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Florescu DF, Sandkovsky U. Cryptosporidium infection in solid organ transplantation. World J Transplant 2016; 6:460-471. [PMID: 27683627 PMCID: PMC5036118 DOI: 10.5500/wjt.v6.i3.460] [Citation(s) in RCA: 32] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 02/10/2016] [Revised: 04/22/2016] [Accepted: 06/16/2016] [Indexed: 02/05/2023] Open
Abstract
Diarrhea is a common complication in solid organ transplant (SOT) recipients and may be attributed to immunosuppressive drugs or infectious organisms such as bacteria, viruses or parasites. Cryptosporidium usually causes self-limited diarrhea in immunocompetent hosts. Although it is estimated that cryptosporidium is involved in about 12% of cases of infectious diarrhea in developing countries and causes approximately 748000 cases each year in the United States, it is still an under recognized and important cause of infectious diarrhea in SOT recipients. It may run a protracted course with severe diarrhea, fluid and electrolyte depletion and potential for organ failure. Although diagnostic methodologies have improved significantly, allowing for fast and accurate identification of the parasite, treatment of the disease is difficult because antiparasitic drugs have modest activity at best. Current management includes fluid and electrolyte replacement, reduction of immunosuppression and single therapy with Nitazoxanide or combination therapy with Nitazoxanide and other drugs. Future drug and vaccine development may add to the currently poor armamentarium to manage the disease. The current review highlights key epidemiological, diagnostic and management issues in the SOT population.
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The Prevalence of Cryptosporidium Oocysts in Birds in Zaria, Nigeria. BORNEO JOURNAL OF RESOURCE SCIENCE AND TECHNOLOGY 2016. [DOI: 10.33736/bjrst.278.2013] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/18/2022] Open
Abstract
A study was conducted to elucidate the prevalence of Cryptosporidium oocysts in birds in Zaria, Nigeria. A total of 890 faecal samples comprising 132, 305 and 453 from wild, local and exotic birds respectively from different parts of Zaria were examined using the formol-ether concentration technique with safranin–methylene blue stain and auramine phenol stain using light microscopy and fluorescent microscopy respectively. The total prevalence rate was 7.4%. However, Samaru had the highest prevalence rate of 20.6% and Tudun Wada the lowest rate of 2.8%. The difference in the prevalence rates between the different localities of Zaria was found to be statistically significant (P<0.001). Among the different birds sampled, local birds had the highest prevalence rate of 9.5% followed by exotic birds 6.6% and the wild ones with 5.3%. The difference was not statistically significant (P>0.05). In Tudun Wada, where the different sexes were noted, there was no significant statistical difference (P>0.05) in the prevalence rate between male and female birds and none between the different species of wild birds sampled (P>0.05). This study confirms the presence of avian Cryptosporidium in Zaria, Nigeria and indicates that whereas location may influence infection, breed, sex and species of birds may not be significant factors in the epidemiology of the infection.
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Molecular investigation of Cryptosporidium in small caged pets in northeast China: host specificity and zoonotic implications. Parasitol Res 2016; 115:2905-11. [PMID: 27107987 DOI: 10.1007/s00436-016-5076-4] [Citation(s) in RCA: 25] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/12/2016] [Accepted: 04/18/2016] [Indexed: 10/21/2022]
Abstract
This study screened 151 pet-derived fecal specimens randomly collected from four commercial markets in northeast China for the presence of Cryptosporidium by genus-specific nested PCRs of the small subunit rRNA gene. Of these, 14 specimens (9.3 %) from nine species of birds, two types of rodents, and a hedgehog were positive for Cryptosporidium. Sequence analysis on the PCR-positive isolates facilitated identification of three Cryptosporidium species (C. baileyi, C. galli, and C. ubiquitum) and two Cryptosporidium genotypes (ferret genotype and avian genotype V). The study birds were affected predominantly with bird-specific C. baileyi (Atlantic canary, budgerigar, crested myna, rock dove, and silky fowl), C. galli (Chinese hwamei), and Cryptosporidium avian genotype V (Fischer's lovebird and rosy-faced lovebird). Cryptosporidium ferret genotype previously considered rodent-adapted was identified in three specimens from budgerigar, chipmunk, and red squirrel. Two specimens collected from common hill myna and hedgehog were positive for C. ubiquitum. The species of birds that can be colonized by Cryptosporidium were extended. Moreover, the data expanded the host range of Cryptosporidium ferret genotype and C. ubiquitum, especially the birds. The carriage of zoonotic C. ubiquitum in small caged pets is of public health importance.
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Public health significance of zoonotic Cryptosporidium species in wildlife: Critical insights into better drinking water management. INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE 2015; 5:88-109. [PMID: 28560163 PMCID: PMC5439462 DOI: 10.1016/j.ijppaw.2015.12.001] [Citation(s) in RCA: 123] [Impact Index Per Article: 12.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/29/2015] [Revised: 12/01/2015] [Accepted: 12/02/2015] [Indexed: 11/22/2022]
Abstract
Cryptosporidium is an enteric parasite that is transmitted via the faecal-oral route, water and food. Humans, wildlife and domestic livestock all potentially contribute Cryptosporidium to surface waters. Human encroachment into natural ecosystems has led to an increase in interactions between humans, domestic animals and wildlife populations. Increasing numbers of zoonotic diseases and spill over/back of zoonotic pathogens is a consequence of this anthropogenic disturbance. Drinking water catchments and water reservoir areas have been at the front line of this conflict as they can be easily contaminated by zoonotic waterborne pathogens. Therefore, the epidemiology of zoonotic species of Cryptosporidium in free-ranging and captive wildlife is of increasing importance. This review focuses on zoonotic Cryptosporidium species reported in global wildlife populations to date, and highlights their significance for public health and the water industry.
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The evolution of respiratory Cryptosporidiosis: evidence for transmission by inhalation. Clin Microbiol Rev 2015; 27:575-86. [PMID: 24982322 DOI: 10.1128/cmr.00115-13] [Citation(s) in RCA: 81] [Impact Index Per Article: 8.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/12/2022] Open
Abstract
The protozoan parasite Cryptosporidium infects all major vertebrate groups and causes significant diarrhea in humans, with a spectrum of diseases ranging from asymptomatic to life-threatening. Children and immunodeficient individuals are disproportionately affected, especially in developing countries, where cryptosporidiosis contributes substantially to morbidity and mortality in preschool-age children. Despite the enormous disease burden from cryptosporidiosis, no antiprotozoal agent or vaccine exists for effective treatment or prevention. Cryptosporidiosis involving the respiratory tract has been described for avian species and mammals, including immunocompromised humans. Recent evidence indicates that respiratory cryptosporidiosis may occur commonly in immunocompetent children with cryptosporidial diarrhea and unexplained cough. Findings from animal models, human case reports, and a few epidemiological studies suggest that Cryptosporidium may be transmitted via respiratory secretions, in addition to the more recognized fecal-oral route. It is postulated that transmission of Cryptosporidium oocysts may occur by inhalation of aerosolized droplets or by contact with fomites contaminated by coughing. Delineating the role of the respiratory tract in disease transmission may provide necessary evidence to establish further guidelines for prevention of cryptosporidiosis.
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Hamidinejat H, Jalali MHR, Jafari RA, Nourmohammadi K. Molecular determination and genotyping of Cryptosporidium spp. in fecal and respiratory samples of industrial poultry in Iran. ASIAN PAC J TROP MED 2014; 7:517-20. [DOI: 10.1016/s1995-7645(14)60086-9] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/10/2014] [Revised: 05/15/2014] [Accepted: 06/15/2014] [Indexed: 10/25/2022] Open
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Zhao GH, Cheng WY, Wang W, Jia YQ, Fang YQ, Du SZ, Yu SK. The expression dynamics of IL-17 and Th17 response relative cytokines in the trachea and spleen of chickens after infection with Cryptosporidium baileyi. Parasit Vectors 2014; 7:212. [PMID: 24886047 PMCID: PMC4036416 DOI: 10.1186/1756-3305-7-212] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/19/2013] [Accepted: 04/28/2014] [Indexed: 01/30/2023] Open
Abstract
BACKGROUND Cryptosporidium baileyi is the dominant Cryptosporidium species in birds causing emerging health problems in the poultry industry, and is also a model to study the biology of Cryptosporidium spp.. IL-17 (also called IL-17A) is a hallmark pro-inflammatory cytokine of Th17 cells that plays an important role in several human autoimmune diseases and microbial infection disease of many animals, and it may play a role in Cryptosporidium infection. METHODS The present study examined the mRNA level of IL-17 and Th17 response relative cytokines in the trachea and spleen of C. baileyi-infected chickens at different time points using real-time quantitative PCR (qPCR). RESULTS All examined cytokines in the trachea were up-regulated in the infected chickens compared with the uninfected control during C. baileyi infection. Significant increased IL-17 mRNA level in the trachea was observed as early as 12 h post infection (pi), peaking at 24 h pi and 10 d pi, and declining thereafter. The transcription levels of IL-17 and Th17 response relative cytokines in spleen were also significantly increased at different time points during the infection. CONCLUSIONS IL-17 was indicated to participate in the induction of inflammation during infection of some intracellular protozoan parasites. The results in the present study suggest that IL-17 may play a role in immunity against Cryptosporidium infection, and provide basic information for determining the role of Th17 cell in Cryptosporidium infection.
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Affiliation(s)
- Guang-Hui Zhao
- College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, People's Republic of China.
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Protozoan Parasites. Food Microbiol 2014. [DOI: 10.1128/9781555818463.ch28] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022]
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Cacciò SM, Widmer G, Axén C, Fayer R. Cryptosporidiosis in Farmed Animals. CRYPTOSPORIDIUM: PARASITE AND DISEASE 2013. [PMCID: PMC7122230 DOI: 10.1007/978-3-7091-1562-6_4] [Citation(s) in RCA: 34] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Subscribe] [Scholar Register] [Indexed: 01/25/2023]
Abstract
Cryptosporidiosis was first identified as a disease of veterinary, rather than human medical, importance, and infection of farmed animals with different species of Cryptosporidium continues to be of veterinary clinical concern. This chapter provides insights into Cryptosporidium infection in a range of farmed animals – cattle, sheep, goats, pigs, cervids, camelids, rabbits, water buffalo and poultry – presenting not only an updated overview of the infection in these animals, but also information on clinical disease, infection dynamics and zoonotic potential. Although extensive data have been accrued on, for example, Cryptosporidium parvum infection in calves, and calf cryptosporidiosis continues to be a major veterinary concern especially in temperate regions, there remains a paucity of data for other farmed animals, despite Cryptosporidium infection causing significant clinical disease and also, for some species, with the potential for transmission of infection to people, either directly or indirectly.
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Affiliation(s)
- Simone M. Cacciò
- Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Rome, Italy
| | - Giovanni Widmer
- Tufts Cummings School of Veterinary Medicine Division of Infectious Diseases, North Grafton, Massachusetts USA
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The first reported cases of human cryptosporidiosis caused by Cryptosporidium hominis in Slovak Republic. Folia Microbiol (Praha) 2012; 58:69-73. [DOI: 10.1007/s12223-012-0182-x] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/21/2012] [Accepted: 07/10/2012] [Indexed: 11/30/2022]
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Bonatti H, Barroso LF, Sawyer RG, Kotton CN, Sifri CD. Cryptosporidium enteritis in solid organ transplant recipients: multicenter retrospective evaluation of 10 cases reveals an association with elevated tacrolimus concentrations. Transpl Infect Dis 2012; 14:635-48. [PMID: 22340660 DOI: 10.1111/j.1399-3062.2012.00719.x] [Citation(s) in RCA: 48] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/23/2011] [Revised: 11/04/2011] [Accepted: 01/09/2012] [Indexed: 02/06/2023]
Abstract
BACKGROUND Cryptosporidial enteritis, a diarrheal infection of the small intestine caused by the apicomplexan protozoa Cryptosporidium, is infrequently recognized in transplant recipients from developed countries. METHODS A retrospective review of all cases of cryptosporidiosis in solid organ transplant (SOT) recipients at 2 centers from January 2001 to October 2010 was performed and compared with transplant recipients with community-onset Clostridium difficile infection (CDI). A literature search was performed with regard to reported cases of cryptosporidiosis in SOT recipients. RESULTS Eight renal, 1 liver, and 1 lung transplant recipient were diagnosed with cryptosporidiosis at median 46.0 months (interquartile range [IQR] 25.2-62.8) following SOT. Symptoms existed for a median 14 days (IQR 10.5-14.8) before diagnosis. For the 9 patients receiving tacrolimus (TAC), mean TAC levels increased from 6.3 ± 1.1 to 21.3 ± 9.2 ng/mL (P = 0.0007) and median serum creatinine increased temporarily from 1.3 (IQR 1.1-1.7) to 2.4 (IQR 2.0-4.6) mg/dL (P = 0.008). By comparison, 8 SOT recipients (6 kidney, 2 liver) hospitalized with community-onset CDI had a mean TAC level of 10.8 ± 2.8 ng/dL during disease compared with 9.2 ± 2.3 ng/mL at baseline (P = 0.07) and had no change in median creatinine. All patients recovered from Cryptosporidium enteritis after receiving various chemotherapeutic regimens. CONCLUSIONS Cryptosporidiosis should be recognized as an important cause of diarrhea after SOT and is associated with elevated TAC levels and acute kidney injury. Increased TAC levels may reflect altered drug metabolism in the small intestine.
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Affiliation(s)
- H Bonatti
- Department of Surgery, University of Virginia Health System, Charlottesville, Virginia, USA
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Putignani L, Menichella D. Global distribution, public health and clinical impact of the protozoan pathogen cryptosporidium. Interdiscip Perspect Infect Dis 2010; 2010:753512. [PMID: 20706669 PMCID: PMC2913630 DOI: 10.1155/2010/753512] [Citation(s) in RCA: 117] [Impact Index Per Article: 7.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/04/2009] [Revised: 01/07/2010] [Accepted: 05/11/2010] [Indexed: 12/19/2022] Open
Abstract
Cryptosporidium spp. are coccidians, oocysts-forming apicomplexan protozoa, which complete their life cycle both in humans and animals, through zoonotic and anthroponotic transmission, causing cryptosporidiosis. The global burden of this disease is still underascertained, due to a conundrum transmission modality, only partially unveiled, and on a plethora of detection systems still inadequate or only partially applied for worldwide surveillance. In children, cryptosporidiosis encumber is even less recorded and often misidentified due to physiological reasons such as early-age unpaired immunological response. Furthermore, malnutrition in underdeveloped countries or clinical underestimation of protozoan etiology in developed countries contribute to the underestimation of the worldwide burden. Principal key indicators of the parasite distribution were associated to environmental (e.g., geographic and temporal clusters, etc.) and host determinants of the infection (e.g., age, immunological status, travels, community behaviours). The distribution was geographically mapped to provide an updated picture of the global parasite ecosystems. The present paper aims to provide, by a critical analysis of existing literature, a link between observational epidemiological records and new insights on public health, and diagnostic and clinical impact of cryptosporidiosis.
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Affiliation(s)
- Lorenza Putignani
- Microbiology Unit, Bambino Gesù Pediatric Hospital, Scientific Institute, Piazza Sant'Onofrio 4, 00165 Rome, Italy
| | - Donato Menichella
- Microbiology Unit, Bambino Gesù Pediatric Hospital, Scientific Institute, Piazza Sant'Onofrio 4, 00165 Rome, Italy
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Willette M, Ponder J, Cruz-Martinez L, Arent L, Bueno Padilla I, de Francisco ON, Redig P. Management of select bacterial and parasitic conditions of raptors. Vet Clin North Am Exot Anim Pract 2009; 12:491-517, Table of Contents. [PMID: 19732706 DOI: 10.1016/j.cvex.2009.06.006] [Citation(s) in RCA: 12] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 05/28/2023]
Abstract
Raptors are susceptible to a broad array of established and emerging bacterial and parasitic diseases, including babesiosis, chlamydiosis, clostridiosis, coccidiosis, cryptosporidiosis, malaria, mycobacteriosis, pasteurellosis, salmonellosis, trichomoniasis, and pododermatitis. Many of these conditions are opportunistic and can be easily managed or averted with proper preventive measures related to captive management, husbandry and diet, and veterinary care. Once infected, treatment must be prompt, appropriate, and judicious. This article examines the significance, diagnosis, management, and prevention of select bacterial and parasitic pathogens of raptors.
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Affiliation(s)
- Michelle Willette
- The Raptor Center, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA.
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Beck HP, Blake D, Dardé ML, Felger I, Pedraza-Díaz S, Regidor-Cerrillo J, Gómez-Bautista M, Ortega-Mora LM, Putignani L, Shiels B, Tait A, Weir W. Molecular approaches to diversity of populations of apicomplexan parasites. Int J Parasitol 2009; 39:175-89. [PMID: 18983997 DOI: 10.1016/j.ijpara.2008.10.001] [Citation(s) in RCA: 75] [Impact Index Per Article: 4.7] [Reference Citation Analysis] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/18/2008] [Revised: 10/14/2008] [Accepted: 10/14/2008] [Indexed: 11/30/2022]
Affiliation(s)
- Hans-Peter Beck
- Swiss Tropical Institute, Socinstrasse 57, CH 4002 Basel, Switzerland.
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Webster KA. Molecular methods for the detection and classification of Cryptosporidium. ACTA ACUST UNITED AC 2005; 9:263-6. [PMID: 15463775 DOI: 10.1016/0169-4758(93)90073-o] [Citation(s) in RCA: 12] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
Abstract
Cryptosporidiosis is a zoonotic disease caused by a parasitic protozoan belonging to the coccidial genus Cryptosporidium. Current laboratory methods are adequate for the detection of the infection when oocysts are present in great numbers, but more-sensitive means of identification are urgently required. In a recent issue of Parasitology Today, Carolyn Petersen has presented a review of the cell biology of this parasite'. Here, Kath Webster draws attention to the various methods involved in its detection and classification.
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Affiliation(s)
- K A Webster
- Central Veterinary Laboratory, Woodham Lane, New Haw, Weybridge, UK KT 15 3NB
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Abstract
Of 155 species of mammals reported to be infected with Cryptosporidium parvum or C. parvum-like organisms most animals are found in the Orders Artiodactyla, Primates, and Rodentia. Because Cryptosporidium from most of these animals have been identified by oocyst morphology alone with little or no host specificity and/or molecular data to support identification it is not known how many of the reported isolates are actually C. parvum or other species. Cryptosporidiosis is a cause of morbidity and mortality in animals and humans, resulting primarily in diarrhea, and resulting in the most severe infections in immune-compromised individuals. Of 15 named species of Cryptosporidium infectious for nonhuman vertebrate hosts C. baileyi, C. canis, C. felis, C. hominis, C. meleagridis, C. muris, and C. parvum have been reported to also infect humans. Humans are the primary hosts for C. hominis, and except for C. parvum, which is widespread amongst nonhuman hosts and is the most frequently reported zoonotic species, the remaining species have been reported primarily in immunocompromised humans. The oocyst stage can remain infective under cool, moist conditions for many months, especially where water temperatures in rivers, lakes, and ponds remain low but above freezing. Surveys of surface water, groundwater, estuaries, and seawater have dispelled the assumption that Cryptosporidium oocysts are present infrequently and in geographically isolated locations. Numerous reports of outbreaks of cryptosporidiosis related to drinking water in North America, the UK, and Japan, where detection methods are in place, indicate that water is a major vehicle for transmission of cryptosporidiosis.
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Affiliation(s)
- Ronald Fayer
- United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.
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Abstract
The genus Cryptosporidium consists of different species and genotypes which infect a wide range of hosts, including humans. The parasite is ubiquitous and lack of differentiation between the species and strains has made it difficult to track down sources of human and animal infections. Genetic analysis of strains and isolates has led to the redescription of Cryptosporidium with special consideration of the host specificity and possible ways of transmission to humans. Infection with the small oocysts usually occurs directly by faecal-oral transmission, water- or food-borne. In Europe water from different sources is frequently contaminated with oocysts. Generally, humans are most frequently infected with C. hominis in an anthroponotic cycle (especially in cases of infections imported from highly endemic (sub-) tropical regions) and the animal genotype (type II) of C. parvum in a zoonotic cycle which seems to play a major role in autochthonous infections in Switzerland, the UK and probably other European countries. Other species (such as C. felis or the avian species C. meleagridis and C. baileyi) and genotypes are rare in humans and mostly restricted to immunocompromised individuals who are highly susceptible to serious opportunistic cryptosporidial infections.
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Affiliation(s)
- A Joachim
- Department für Pathobiologie, Institut für Parasitologie und Zoologie, Veterinärmedizinische Universität Wien, Veterinärplatz 1, A-1210 Vienna, Austria.
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Abe N, Iseki M. Identification of Cryptosporidium isolates from cockatiels by direct sequencing of the PCR-amplified small subunit ribosomal RNA gene. Parasitol Res 2004; 92:523-6. [PMID: 14999470 DOI: 10.1007/s00436-004-1082-z] [Citation(s) in RCA: 25] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/08/2003] [Accepted: 01/13/2004] [Indexed: 10/26/2022]
Abstract
Cryptosporidium is a significant pathogen in humans and animals. Cases of infection by C. meleagridis or C. baileyi with zoonotic potential have also been reported in domestic birds; and recent studies indicate the presence of new host-adapted species or genotype in birds. Therefore, accurately identifying isolates is important for understanding the epizootiology of Cryptosporidium infection in birds and for the control of human cryptosporidiosis. Cryptosporidium has been detected in cockatiels, but the species or genotype of isolates remains unclear because identification was performed using conventional microscopy. We report herein the species or genotype of isolates from two cockatiels distinguished by a PCR-based diagnostic method. The isolates were found to be C. meleagridis and C. baileyi, respectively. This study documents the first discovery of C. meleagridis and C. baileyi in cockatiels and suggests that pet birds may play an important role in the epidemiology of cryptosporidiosis.
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Affiliation(s)
- Niichiro Abe
- Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Tennoji-ku, 543-0026 Osaka, Japan.
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Clancy JL, Marshall MM, Hargy TM, Korich DG. Susceptibility of five strains of Cryptosporidium parvum oocysts to UV light. ACTA ACUST UNITED AC 2004; 96:84-93. [PMID: 32313290 PMCID: PMC7159772 DOI: 10.1002/j.1551-8833.2004.tb10576.x] [Citation(s) in RCA: 27] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/03/2022]
Abstract
Previous evaluations of the effect of ultraviolet (UV) light on Cryptosporidium parvum oocysts have been limited to a single strain—the Iowa strain. This study investigated the response of five strains of C. parvum to UV. A collimated beam apparatus was used to apply controlled doses of monochromatic (254 nm) UV to oocysts of the Iowa, Moredun, Texas A&M, Maine, and Glasgow strains. Irradiation was measured using a calibrated radiometer and sensor. Inactivation was quantified through animal infectivity by inoculation of cohorts of CD‐1 neonatal mice with UV‐treated and untreated control oocysts of each strain followed by examination of intestinal sections for infection using hemotoxylin and eosin staining. A UV light dose of 10 mJ/cm2 achieved at least 4‐log10 inactivation of all strains evaluated. All five strains of C. parvum were shown to be highly susceptible to low levels of UV light.
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Abstract
OBJECTIVES To review and assess the role of electron microscopy in the investigation of new infectious diseases. DESIGN To design a screening strategy to maximize the likelihood of detecting new or emerging pathogens in clinical samples. RESULTS Electron microscopy remains a useful method of investigating some viral infections (infantile gastroenteritis, virus-induced outbreaks of gastroenteritis and skin lesions) using the negative staining technique. In addition, it remains an essential technique for the investigation of new and emerging parasitic protozoan infections in the immunocompromised patients from resin-embedded tissue biopsies. Electron microscopy can also have a useful role in the investigation of certain bacterial infections. CONCLUSIONS Electron microscopy still has much to contribute to the investigation of new and emerging pathogens, and should be perceived as capable of producing different, but equally relevant, information compared to other investigative techniques. It is the application of a combined investigative approach using several different techniques that will further our understanding of new infectious diseases.
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Affiliation(s)
- Alan Curry
- Electron Microscopy Unit, Manchester Royal Infirmary, Central Manchester Healthcare Trust, Manchester, UK.
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Abe N, Kimata I, Iseki M. Identification of genotypes of Cryptosporidium parvum isolates from a patient and a dog in Japan. J Vet Med Sci 2002; 64:165-8. [PMID: 11913556 DOI: 10.1292/jvms.64.165] [Citation(s) in RCA: 39] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022] Open
Abstract
Cryptosporidium parvum (C. parvum) is recognized as a significant pathogen in humans and animals, primarily as a cause of diarrheal illness. Recent genetic and biological studies indicate that C. parvum is not a single species but composed of genetically distinct multiple genotypes. Thus, it is valuable to distinguish between genotypes in the epidemiology of Cryptosporidium infection in humans and animals. Although C. parvum has been detected in humans and animals in Japan, the genotype of isolates remains unclear because identification has been performed only by conventional microscopy. We report herein the genotypes of C. parvum isolates distinguished by the polymerase chain reaction (PCR)-based diagnostic method. C. parvum isolates, originally obtained from a patient and a pet dog, were found to have cattle and dog genotypes, respectively.
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Affiliation(s)
- Niichiro Abe
- Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Japan
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Hunter PR, Nichols G. Epidemiology and clinical features of Cryptosporidium infection in immunocompromised patients. Clin Microbiol Rev 2002; 15:145-54. [PMID: 11781272 PMCID: PMC118064 DOI: 10.1128/cmr.15.1.145-154.2002] [Citation(s) in RCA: 309] [Impact Index Per Article: 13.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/01/2023] Open
Abstract
Cryptosporidium spp. are a major cause of diarrheal disease in both immunocompetent and immunodeficient individuals. They also cause waterborne disease in both the United States and United Kingdom. Studies on the mechanisms of immunity to cryptosporidiosis indicate the importance of the T-cell response. The spectrum and severity of disease in immunocompromised individuals with cryptosporidiosis reflect this importance since the most severe disease is seen in individuals with defects in the T-cell response. The most commonly studied group is that of patients with AIDS. These patients suffer from more severe and prolonged gastrointestinal disease that can be fatal; in addition, body systems other than the gastrointestinal tract may be affected. The widespread use of antiretroviral therapy does appear to be having a beneficial effect on recovery from cryptosporidiosis and on the frequency of infection in human immunodeficiency virus-positive patients. Other diseases that are associated with increased risk of severe cryptosporidiosis, such as primary immunodeficiencies, most notably severe combined immunodeficiency syndrome, are also predominantly associated with T-cell defects. Of the remaining groups, children with acute leukemia seem to be most at risk from cryptosporidiosis. There is less evidence of severe complications in patients with other malignant diseases or in those receiving immunosuppressive chemotherapy.
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Affiliation(s)
- Paul R Hunter
- School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, United Kingdom.
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Kuhn RC, Rock CM, Oshima KH. Occurrence of Cryptosporidium and Giardia in wild ducks along the Rio Grande River valley in southern New Mexico. Appl Environ Microbiol 2002; 68:161-5. [PMID: 11772622 PMCID: PMC126547 DOI: 10.1128/aem.68.1.161-165.2002] [Citation(s) in RCA: 34] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022] Open
Abstract
Fecal samples were taken from wild ducks on the lower Rio Grande River around Las Cruces, N. Mex., from September 2000 to January 2001. Giardia cysts and Cryptosporidium oocysts were purified from 69 samples by sucrose enrichment followed by cesium chloride (CsCl) gradient centrifugation and were viewed via fluorescent-antibody (FA) staining. For some samples, recovered cysts and oocysts were further screened via PCR to determine the presence of Giardia lamblia and Crytosporidium parvum. The results of this study indicate that 49% of the ducks were carriers of Cryptosporidium, and the Cryptosporidium oocyst concentrations ranged from 0 to 2,182 oocysts per g of feces (mean +/- standard deviation, 47.53 +/- 270.3 oocysts per g); also, 28% of the ducks were positive for Giardia, and the Giardia cyst concentrations ranged from 0 to 29,293 cysts per g of feces (mean +/- standard deviation, 436 +/- 3,525.4 cysts per g). Of the 69 samples, only 14 had (oo)cyst concentrations that were above the PCR detection limit. Samples did test positive for Cryptosporidium sp. However, C. parvum and G. lamblia were not detected in any of the 14 samples tested by PCR. Ducks on their southern migration through southern New Mexico were positive for Cryptosporidium and Giardia as determined by FA staining, but C. parvum and G. lamblia were not detected.
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Affiliation(s)
- Ryan C Kuhn
- Department of Biology, New Mexico State University, Las Cruces, New Mexico 88003, USA
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Lindergard G, Wade SE, Schaaf S, Barwick RS, Mohammed HO. Detection of Cryptosporidium oocysts in soil samples by enzyme-linked immunoassay. Vet Parasitol 2001; 94:163-76. [PMID: 11113547 DOI: 10.1016/s0304-4017(00)00379-4] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/23/2022]
Abstract
An ELISA protocol was adapted for detection of Cryptosporidium parvum oocysts in soil samples and the limit of detection of the test was determined. A modified indirect antigen capture ELISA protocol was developed using monoclonal antibodies against the oocyst outer wall. The accuracy of the ELISA was compared to spiked soil samples and measured in terms of sensitivity and specificity of the test. The performance of the ELISA was evaluated in field soil samples and measured using the kappa-statistics. Similarly, the performance of the ELISA was compared to the concentration flotation method, to a modified concentration flotation method and to a commercial ELISA (ProSpecT) in field fecal and soil samples. The limit of detection of the test was selected to be 10,000 oocysts/g. At this limit of detection, the ELISA had a sensitivity of 95% and specificity of 100%. The agreement between the ELISA and the modified flotation-concentration method in detecting Cryptosporidium oocysts in soil samples was 32% (kappa=0.32). The ELISA had the same relative sensitivity (82%) in comparison to both the flotation and ProSpecT in determining Cryptosporidium-infection status of an animal. The kappa-statistics was 0.26 for both tests. The developed ELISA proved to be a valuable diagnostic test for detecting oocysts in soil samples and has a potential application in determining the infection status of animals.
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Affiliation(s)
- G Lindergard
- Department of Population Medicine and Diagnostic Science, Section of Epidemiology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
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Yu JR, Choi SD. The effect of microfilament inhibitor on the Cryptosporidium infection in vitro. THE KOREAN JOURNAL OF PARASITOLOGY 2000; 38:257-61. [PMID: 11138319 PMCID: PMC2721208 DOI: 10.3347/kjp.2000.38.4.257] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/23/2022]
Abstract
This study was focused on the effects of microfilament inhibitor, Cytochalasin D (CD) on the invasiveness of sporozoites of Cryptosporidium spp. into the host cells. MDCK and AGS cell lines were used as host cells for C. parvum and C. muris, respectively. When MDCK cells were pretreated with CD for 1 hr before inoculation of the sporozoites, C. parvum infection was significantly inhibited when compared to the control cells. These inhibitory effects of CD on the rate of infection were dose-dependent. In addition, C. muris infection was hampered when AGS cell lines were pretreated with CD. However, the capability of invasiveness of the sporozoites into the host cells was not greatly influenced by the pretreatment of sporozoites with CD before infection. These results suggest that microfilaments of host cells, rather than parasites, play an important role for the invasion of Cryptosporidium spp.
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Affiliation(s)
- J R Yu
- Department of Parasitology, College of Medicine, Konkuk University, Chungju 380-701, Korea.
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Abstract
Electron microscopy (EM) is still an important tool for the investigation of infectious diseases, despite the introduction of powerful new methods, mainly involving the polymerase chain reaction. Particularly in the field of parasitic protozoology associated with AIDS, where many new species of human pathogens have been recognized in tissue biopsies, EM remains an essential 'catch-all' diagnostic method. The resolved ultrastructural details of these newly recognized parasites allows a unique insight into the biology of these organisms. The information produced by EM is different, but complementary, to that provided by alternative methods.
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Affiliation(s)
- A Curry
- Public Health Laboratory, Withington Hospital, Manchester, UK
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Abstract
The morphology, life cycle, maintenance, host specificity, incidence of Cryptosporidium species infecting birds, as well as the epidemiology, clinical signs, pathology, immunology, diagnosis, therapy, and control of avian cryptosporidiosis are reviewed. Based on the accepted criteria used for differentiation of Cryptosporidium isolates into valid species, this review places the validity of C. meleagridis in doubt and suggests that C. meleagridis isolated from birds is very closely related to, or identical with C. parvum infecting more than 100 species of mammals.
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Affiliation(s)
- T Sréter
- Department of Parasitology, University of Veterinary Science, Budapest, Hungary.
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Xiao L, Morgan UM, Limor J, Escalante A, Arrowood M, Shulaw W, Thompson RC, Fayer R, Lal AA. Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Appl Environ Microbiol 1999; 65:3386-91. [PMID: 10427023 PMCID: PMC91508 DOI: 10.1128/aem.65.8.3386-3391.1999] [Citation(s) in RCA: 435] [Impact Index Per Article: 16.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022] Open
Abstract
To assess the genetic diversity in Cryptosporidium parvum, we have sequenced the small subunit (SSU) rRNA gene of seven Cryptosporidium spp., various isolates of C. parvum from eight hosts, and a Cryptosporidium isolate from a desert monitor. Phylogenetic analysis of the SSU rRNA sequences confirmed the multispecies nature of the genus Cryptosporidium, with at least four distinct species (C. parvum, C. baileyi, C. muris, and C. serpentis). Other species previously defined by biologic characteristics, including C. wrairi, C. meleagridis, and C. felis, and the desert monitor isolate, clustered together or within C. parvum. Extensive genetic diversities were present among C. parvum isolates from humans, calves, pigs, dogs, mice, ferrets, marsupials, and a monkey. In general, specific genotypes were associated with specific host species. A PCR-restriction fragment length polymorphism technique previously developed by us could differentiate most Cryptosporidium spp. and C. parvum genotypes, but sequence analysis of the PCR product was needed to differentiate C. wrairi and C. meleagridis from some of the C. parvum genotypes. These results indicate a need for revision in the taxonomy and assessment of the zoonotic potential of some animal C. parvum isolates.
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Affiliation(s)
- L Xiao
- Division of Parasitic Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30341, USA.
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Bornay-Llinares FJ, da Silva AJ, Moura IN, Myjak P, Pietkiewicz H, Kruminis-Lozowska W, Graczyk TK, Pieniazek NJ. Identification of Cryptosporidium felis in a cow by morphologic and molecular methods. Appl Environ Microbiol 1999; 65:1455-8. [PMID: 10103236 PMCID: PMC91206 DOI: 10.1128/aem.65.4.1455-1458.1999] [Citation(s) in RCA: 72] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022] Open
Abstract
Apicomplexan Cryptosporidium parasites infect a wide range of vertebrate hosts. While some species are limited to a single host group, such as Cryptosporidium baileyi, which infects chickens, other species of this genus, such as C. parvum, infect a wide range of mammalian species from mice to humans. During an investigation of Cryptosporidium infection in cattle on a farm in northern Poland, we identified an infection caused by C. felis, in addition to known infections with C. muris and C. parvum. This new infection was identified based on the size of the oocysts (mean size, 4.3 +/- 0.4 micrometer; range, 3.5 to 5.0 micrometer), as well as by analysis of the molecular sequence of the variable region of the small-subunit rRNA. This finding demonstrates the complex host specificity and circulation in the environment of Cryptosporidium species.
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Affiliation(s)
- F J Bornay-Llinares
- Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia
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Taylor MA, Webster KA. Recent advances in the diagnosis in livestock of Cryptosporidium, Toxoplasma, Giardia and other protozoa of veterinary importance. Res Vet Sci 1998; 65:183-93. [PMID: 9915141 PMCID: PMC7131700 DOI: 10.1016/s0034-5288(98)90141-2] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 05/21/1998] [Indexed: 01/23/2023]
Affiliation(s)
- M A Taylor
- Parasitology Discipline, Central Veterinary Laboratory, (Veterinary Laboratories Agency), New Haw, Addlestone, Surrey
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