Tanshinone IIA improves Alzheimer’s disease via RNA nuclear-enriched abundant transcript 1/microRNA-291a-3p/member RAS oncogene family Rab22a axis

Table 1 Sequences of the three small interfering RNAs targeting Rab22a and a microRNA-291a-3p mimic or inhibitor

	Sequences of si-Rab22a 5’-3’
si-Rab22a-1	GGAAATGATCACAAGTAGAGG
si-Rab22a-2	GGAAATGGTAATAAAGACATA
si-Rab22a-3	CGATCTTACTGATGTCAGAGA
si-NC	TTCTCCGAACGTGTCACGTTT
	Sequences of the miR-291a-3p mimic and inhibitor 5’-3’
Mimic NC	ATTGATTTGTTCCGAAGGCCCT
miR-291a-3p mimic	AAAGTGCTTCCACTTTGTGTGC
Inhibitor NC	TCGCTCTATCCTGATCGAATGAA
miR-291a-3p inhibitor	GCACACAAAGTGGAAGCACTTT

Table 2 Primer sequences used for reverse transcription quantitative polymerase chain reaction analysis

Primers	Forward primer 5’-3’	Reverse primer 5’-3’
NEAT1	TGGAGATTGAAGGCGCAAGT	AAGCACGGAACCTAGGCAAA
miR-291a-3p	ACACTCCAGCTGGGAAAGTGCTTCCACTTT	CTCAACTGGTGTCGTGGA
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT
Rab22a	ATCAATCCAACCATAGGGGCAT	TTGGTGCCAATGCACGAAATC
GAPDH	GTGGCAAAGTGGAGATTGTTGCC	GATGATGACCCGTTTGGCTCC

U6 was used to normalize miR-291a-3p expression, and GAPDH was used to normalize nuclear-enriched abundant transcript 1 mRNA expression. NEAT1: Nuclear-enriched abundant transcript 1.

Table 3 Differentially expressed long non-coding RNAs in the GSE150696 dataset

Gene symbol	Fold change	P value
NEAT1	2.19	0.0170
LINC01377	1.98	0.0248
EYA transcriptional coactivator and phosphatase 3- intronic transcript 1	1.95	0.0027
LINC01354	1.68	0.0119
LINC00537	1.64	0.0498
LINC01441	1.63	0.0082
LINC01094	1.63	0.0328
Cancer susceptibility 16	1.6	0.0376
LINC01101	1.55	0.0342
LINC00327	1.54	0.0028
LINC00323	1.54	0.0459
LINC00644	1.53	0.0119
LINC00358	1.53	0.0411
LINC00347	1.51	0.0409
LINC00641	-1.53	0.0332
LINC00294	-1.55	0.0471
Maternally expressed 8	-1.56	0.0073
Prader-Willi region non-protein coding RNA 1	-1.59	0.0005
LINC00672	-1.66	0.0010
LINC00969	-1.72	0.0302
Prostate androgen-regulated transcript 1	-1.8	0.0049
LINC00622	-1.8	0.0175
LINC00507	-1.81	0.0000625
LINC00403	-1.84	0.0043
LINC01128	-1.87	0.0179
LINC00889	-1.92	0.0113
LINC00473	-2.43	0.0323
LINC00622	-2.5	0.0037

LINC: Long intergenic long non-coding RNA; NEAT1: Nuclear-enriched abundant transcript 1.