Identification and characterization of noncoding RNAs-associated competing endogenous RNA networks in major depressive disorder

Figure 1 Flowchart of the study. A: Step 1, dataset screening; B: Step 2, competing endogenous RNA network construction. DEGs: Differentially Expressed Genes; mRNA: Messenger RNA; miRNA: MicroRNA; lncRNA: Long non-coding RNA; ceRNA: Competing endogenous RNAs; miRcode: http://www.mircode.org/; miRDB: http://mirdb.org/; TargetScan: http://www.targetscan.org/vert_72/; DE_mRNA: Differentially expressed mRNA; DE_lncRNA: Differentially expressed lncRNA; DE_miRNA: Differentially expressed miRNA.

Figure 2 Difference and cluster analyses. A: Volcanic maps of differentially expressed genes in GSE52790; B: Cluster analysis heatmap of the expression patterns of the differentially expressed genes in GSE52790; C: Volcanic maps of differentially expressed genes in GSE76826; D: Cluster analysis heatmap of the expression patterns of the differentially expressed genes in GSE76826; E: Volcanic maps of differentially expressed genes in GSE81152; F: Cluster analysis heatmap of the expression patterns of the differentially expressed genes in GSE81152. Red indicates upregulated genes; green indicates downregulated genes.

Figure 3 Major depressive disorder-associated enriched competing endogenous RNA network. Red and green indicate upregulated downregulated genes, respectively. The circles represent differentially expressed RNA (DE_RNA), triangles represent DE long noncoding RNA (DE_lncRNA), and rounded squares represent DE_microRNA.

Figure 4  Gene Ontology functional enrichment analysis.

Figure 5 Protein interaction network construction. FA2H: Fatty acid 2-hydroxylase.