Nutritional intake is one of the most common environmental risk factors of polycystic ovary syndrome (PCOS) because it is associated with obesity and insulin resistance. This study aims to determine the relationship between micronutrient intake and androgen levels associated with PCOS.

This cross-sectional study was performed on 79 PCOS patients, consisted of 50 normoandrogenic (NA) and 29 hyperandrogenic (HA) patients and 66 healthy controls. Dietary intake assessment was performed using a modified 38-item semi-quantitative food frequency questionnaire. Bivariate, correlation, and multivariate analyses were performed to determine the association between study variables, and p-value less than 0.05 was considered as statistically significant difference.

The baseline characteristics in all groups were similar, except for body mass index and hormonal profile, compared to those in the other groups, found to be higher in the hyperandrogenic PCOS group. There was found a significantly negative correlation between the free androgen index and intake of vitamin B1, vitamin B2, niacin, vitamin B6, calcium, and iron in the normoandrogenic PCOS group, while we did not observe it in the hyperandrogenic PCOS group. Multivariate linear regression analysis reveals that the intake of vitamin B6, vitamin C, niacin, and iron had a significant effect on the free androgen index.

There is an effect of micronutrient intake on androgen levels in women with PCOS. The association was more significant in the normoandrogenic PCOS than in the hyperandrogenic PCOS. These findings reveal an association between micronutrients and androgens and PCOS at a systemic level.

Polycystic ovary syndrome (PCOS) is an increasingly common problem for women in the reproductive age throughout the entire world. A reduction diet with a low glycaemic index (GI) has proved to support the treatment of PCOS. The aim of the study was to analyse the influence of the diet on the level of vitamins soluble in water. The study included 55 women, 40 of which suffered from PCOS (identified by means of the Rotterdam Criteria) and 15 healthy women of the Caucasian race. The level of vitamins before and after the dietary intervention was measured. The diet was a reduction diet with a reduced glycaemic index (GI). Biochemical analyses were made on the basis of liquid chromatography-Infinity 1260 Binary liquid chromatography (LC) Agilent Technology. The level of vitamins in the serum was analysed together with the consumption before and after the dietary intervention. A higher level of vitamin C in the plasma was observed before and after the dietary intervention in the PCOS group in comparison to the control group despite the lower intake of this vitamin in the PCOS group. The remaining vitamins were at a comparable or lower level (B1, B3, B5, B6 and B12). After the dietary intervention, only B1 and B9 were at a clearly lower level (a trend of p = 0.093 and p = 0.085). A properly balanced reduction diet with reduced GI improves the supply of vitamins in women with PCOS. An additional recommendation should be the additional supplementation of B1, niacinamide and the combination of folates with inositol. The level of vitamin C in the plasma may not be a good marker of its supply in the PCOS group.

The effect of folic acid in mitigating depression has remained pivotal in research.

To determine the effects of folate supplementation on neurobehaviour oxidative stress and cerebral cortex histomorphology in the dexamethasone mouse model of depression.

Male mice were assigned to six groups (A-F) of 10 mice each. Animals in groups A and D were fed a standard diet, while those in B and E were fed folic acid supplemented diet (25 mg/kg of feed), while C and F were fed folate supplemented diet at 50 mg/kg of feed for 8 weeks. At the beginning of the sixth 6th week, mice in groups A-C were administered distilled water, while animals in groups D-F were administered dexamethasone (DEX) at 4 mg/kg body weight by gavage. Open-field, forced swim, and tail-suspension tests were conducted at the end of the experimental period, following which animals were euthanised and blood was taken for the estimation of Malondialdehyde (MDA), reduced Glutathione, Glutathione Peroxidase, Catalase activity, and Superoxide Dismutase. Sections of the cerebral cortex were prepared for histological examination.

Folic acid supplementation increased body weight, locomotor, rearing and self-grooming behaviours, and decreased immobility time in the tail suspension and forced swim tests. There was also a reduction of lipid peroxidation and an increase in the antioxidant status. Folic acid supplementation was also found to be protective against the development of dexamethasone-induced changes in body weight, open-field behaviours, behavioural despair, oxidative stress and cerebrocortical morphology.

Folic-acid supplementation improves the behavioral, some antioxidant, and cerebral morphological parameters.