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1
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Li Z, Wang M, Yang Z. Analysis of the association between microbiota and flavor formation during Zizhong Dongjian fermentation process. Food Sci Nutr 2024; 12:9493-9510. [PMID: 39619991 PMCID: PMC11606816 DOI: 10.1002/fsn3.4460] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/02/2024] [Revised: 08/22/2024] [Accepted: 08/29/2024] [Indexed: 03/17/2025] Open
Abstract
Zizhong Dongjian (ZZDJ) is one of the most famous and popular fermented vegetables in China. The aim of this study was to explore the microbial communities and volatile flavor compounds of ZZDJ during different fermentation periods, as well as to reveal the potential correlation between microbiota and flavor. A total of 84 volatile flavor compounds were detected in 0-year to 3-year ZZDJ samples. Hydrocarbons were the most abundant flavor compounds in 0-year and 1-year samples, while esters became the predominant flavor components in 2-year and 3-year samples. Furthermore, Loigolactobacillus, Pseudomonas, and Virgibacillus were most predominant bacteria during the fermentation process of ZZDJ. Interestingly, all the fungi identified were yeasts. Among them, Zygosaccharomyces and Symmetrospora dominated alternatively throughout the fermentation process of ZZDJ. Through analysis of relativity between flavor compounds and microorganism of ZZDJ, we found that Uncultured Pseudomonas sp., Virgibacillus sediminis, Zygosaccharomyces rouxii, and Symmetrospora marina might play important roles in flavor information of ZZDJ.
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Affiliation(s)
- Zhang Li
- Laboratory of Molecular Oncology, Frontiers Science Center for Disease‐related Molecular Network, State Key Laboratory of Biotherapy and Cancer Center, West China HospitalSichuan UniversityChengduChina
| | - Miao Wang
- Laboratory Animal Center, West China School of Basic Medical Science & Forensic MedicineSichuan UniversityChengduChina
| | - Zhirong Yang
- Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life SciencesSichuan UniversityChengduChina
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2
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Bavaro AR, Tarantini A, Bruno A, Logrieco AF, Gallo A, Mita G, Valerio F, Bleve G, Cardinali A. Functional foods in Mediterranean diet: exploring the functional features of vegetable case-studies obtained also by biotechnological approaches. Aging Clin Exp Res 2024; 36:208. [PMID: 39412623 PMCID: PMC11485090 DOI: 10.1007/s40520-024-02860-1] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/30/2024] [Accepted: 09/28/2024] [Indexed: 10/19/2024]
Abstract
The Mediterranean Diet (MedDiet) is a widely recognized dietary pattern, with its effects largely attributed to "functional foods" which are able to positively influence one or more target functions, improving health and maintaining a state of well-being.In this review, three "case-study" typical of the MedDiet, such as artichokes, capers and table olives are considered as traditional functional vegetables rich in bioactive compounds, mainly polyphenols. The review extensively discusses the antioxidant effects of these molecules, as well as their role in aging prevention and reduction, maintaining human health, and influencing the abundance and composition of intestinal microbiota. Additionally, this review focuses on the fate of the dietary polyphenols along the digestive tract.Among biotechnological strategies, the review explores the role of fermentation process in modifying the biochemical profile, recovery, bioaccessibility and bioavailability of bioactive compounds present in some vegetable foods of MedDiet. Finally, the main challenges in the selection, addition, and maintenance of probiotic strains in traditional food products are also summarized, with a view to develop new probiotic carriers for "functional diets".
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Affiliation(s)
- Anna Rita Bavaro
- National Research Council, Institute of Sciences of Food Production (CNR-ISPA), Bari, 70126, Italy
| | - Annamaria Tarantini
- National Research Council, Institute of Sciences of Food Production (CNR-ISPA), Lecce, 73100, Italy
| | - Angelica Bruno
- National Research Council, Institute of Sciences of Food Production (CNR-ISPA), Bari, 70126, Italy
| | - Antonio F Logrieco
- National Research Council, Institute of Sciences of Food Production (CNR-ISPA), Bari, 70126, Italy
- Xianghu Lab, Biomanufactoring Institute, Hangzhou, Zhejiang, China
| | - Antonia Gallo
- National Research Council, Institute of Sciences of Food Production (CNR-ISPA), Lecce, 73100, Italy
| | - Giovanni Mita
- National Research Council, Institute of Sciences of Food Production (CNR-ISPA), Lecce, 73100, Italy
| | - Francesca Valerio
- National Research Council, Institute of Sciences of Food Production (CNR-ISPA), Bari, 70126, Italy.
| | - Gianluca Bleve
- National Research Council, Institute of Sciences of Food Production (CNR-ISPA), Lecce, 73100, Italy.
| | - Angela Cardinali
- National Research Council, Institute of Sciences of Food Production (CNR-ISPA), Bari, 70126, Italy
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3
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Alghamdi AH, Ahmed AA, Abdalgadir H, Bashir M, Khalid A, Abdalla AN, Elzubier ME, Adnan Almaimani R, Refaat B, Alzahrani K, Alghamdi SM, Gul S. In-vitro Cytotoxicity Investigations for Phytoconstituents of Saudi Medicinal Plants With Putative Ocular Effects. Integr Cancer Ther 2024; 23:15347354241256649. [PMID: 38819027 PMCID: PMC11143859 DOI: 10.1177/15347354241256649] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/15/2023] [Revised: 04/20/2024] [Accepted: 05/06/2024] [Indexed: 06/01/2024] Open
Abstract
BACKGROUND Metastatic secondary ocular tumors spread from systemic malignancies, including breast cancer. This study aimed to evaluate the cytotoxicity of extracts from 5 medicinal plants native to Saudi Arabia. METHODS For preliminary activity screening, cytotoxicity using the MTT assay and selectivity index determinations were made for medicinal plant extracts against various cancer cell-lines. The most promising extract was subjected to GC-MS analysis to determine the phytochemical composition. Clonogenic assays were performed using the most promising extract to confirm the initial results. Finally, western blot analysis was used to determine the modulation in expression of survivin and P27 suppressor genes in the human breast adenocarcinoma (MCF7) cell-line to understand the potential mechanistic properties of the active plant extract. RESULTS The 5 plant extracts showed various cytotoxic activity levels using IC50. The most active extract was found to be the leaves of Capparis spinosa L. (BEP-07 extract) against the MCF7 breast cancer cell-line (IC50 = 3.61 ± 0.99 μg/ml) and selectivity index of 1.17 compared to the normal human fetal lung fibroblast (MRC5) cells. BEP-07 extract showed a dose dependent clonogenic effect against the MCF7 colonies which was comparable with the effect of doxorubicin. BEP-07 extract caused a significant decrease of survivin and increase in P27 expression compared to control GAPDH at its highest dose (14 µg/ml). The GC-MS chromatogram of Capparis spinosa L. (BEP-07 extract) revealed the existence of 145 compounds, belonging to the diverse classes of phytoconstituents. Fatty acids and their derivatives represent 15.4%, whilst octadecanoic acid, 2,3-dihydroxypropyl ester was the principal component (7.9%) detected. CONCLUSION Leaves of Capparis spinosa L. (BEP-07 extract) exhibited a significant cytotoxic effect particularly against breast cancer cells. It exhibited this effect through survivin inhibition and via P27 upregulation. The detected phytoconstituents in the plant extract might be involved in tested cytotoxic activity, while further investigations are required to complete the drug candidate profile.
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Affiliation(s)
| | - Aimun A.E. Ahmed
- Al Baha University, Al Baha, Saudi Arabia
- Omdurman Islamic University, Khartoum, Sudan
| | | | | | | | - Ashraf N. Abdalla
- Umm Al-Qura University, Makkah, Saudi Arabia
- National Center for Research, Khartoum, Sudan
| | | | | | | | | | | | - Sheraz Gul
- Fraunhofer Institute for Translational Medicine and Pharmacology, Hamburg, Germany
- Fraunhofer Cluster of Excellence for Immune-Mediated Diseases CIMD, Hamburg, Germany
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4
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Hanuš L, Naor T, Gloriozova T, Dembitsky VM. Natural isothiocyanates of the genus Capparis as potential agonists of apoptosis and antitumor drugs. World J Pharmacol 2023; 12:35-52. [DOI: 10.5497/wjp.v12.i4.35] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/30/2023] [Revised: 10/12/2023] [Accepted: 11/24/2023] [Indexed: 12/19/2023] Open
Abstract
BACKGROUND Using gas chromatography-mass spectrometry (GC/MS) analysis, we examined the composition of volatile components present in the yellow and green fruits, seeds, and jam of the scrambling shrub Capparis cartilaginea (C. cartilaginea). These plant samples were collected from Kibbutz Yotvata in Israel. In all the tested samples, isothiocyanates were identified. Utilizing the PASS program, we ascertained the biological activity of these isothiocyanates present in the Capparis genus. The study results highlighted that all isothiocyanates could potentially act as apoptosis agonists, making them strong candidates for antitumor drugs. This information holds significant value for the fields of medicinal chemistry, pharmacology, and practical medicine.
AIM To investigate the volatile components present in the yellow and green fruits, seeds, and jam of the C. cartilaginea shrub using GC/MS analysis, to detect isothiocyanates in all the analyzed plant samples, and to assess the biological activity of these isothiocyanates utilizing the PASS program.
METHODS We utilized two primary methods to analyze the volatile compounds present in the yellow and green fruits, seeds, and jams of the C. cartilaginea, native to Israel. We identified biologically active isothiocyanates in these samples. Their anticipated biological activities were determined using the PASS program, with the most dominant activities being apoptosis agonist, anticarcinogenic, and antineoplastic specifically for genitourinary cancer.
RESULTS Fruits, seeds, and jams containing isothiocyanates, which exhibit antineoplastic and anticarcinogenic activities, could be suggested for cancer prevention and management. Specific isothiocyanates, with therapeutic potential in this realm, could be recommended as potent anticancer agents in practical medicine following clinical trials.
CONCLUSION The discovery that isothiocyanates exhibit potent antineoplastic and anticarcinogenic activities was unexpected. Additionally, certain isothiocyanates demonstrated antifungal, antiviral (specifically against arbovirus), and antiparasitic properties.
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Affiliation(s)
- Lumír Hanuš
- Institute for Drug Research, School of Pharmacy, Faculty of Medicine, Hebrew University, Ein Kerem Campus, Jerusalem 91120, Israel
| | - Tuvia Naor
- Food Chemistry, Kibbutz, Yotvata 8882000, Israel
| | - Tatyana Gloriozova
- Department of Bioinformatics, Institute of Biomedical Chemistry, Moscow 119121, Russia
| | - Valery M Dembitsky
- Centre for Applied Research and Innovation, Lethbridge College, Lethbridge AB T1K 1L6, Canada
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5
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Capparis spinosa L. as a potential source of nutrition and its health benefits in foods: A comprehensive review of its phytochemistry, bioactivities, safety, and application. Food Chem 2023; 409:135258. [PMID: 36587515 DOI: 10.1016/j.foodchem.2022.135258] [Citation(s) in RCA: 5] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/06/2022] [Revised: 11/29/2022] [Accepted: 12/18/2022] [Indexed: 12/24/2022]
Abstract
Capparis spinosa L. (C. spinosa) is an edible plant with health-promoting benefits. C. spinosa possesses various biological activities, including antioxidant, antidiabetic, anticancer, hepatoprotective, neuroprotective, anti-inflammatory, anti-arthritic, antibacterial, and insecticidal effects. The active compounds associated with these effects mainly include flavonoids, phenolic acids, alkaloids, volatile oils, fatty acids, and polysaccharides. Moreover, C. spinosa has considerable nutritional value. Apart from being a food condiment, it belongs to a class of functional ingredients that act as preservatives and antioxidants in food products. C. spinosa has also shown good potential applications in novel food packaging materials. In this article, in addition to systematically reviewing the botanical characteristics, traditional edible uses, phytochemical composition, bioactivities and safety of C. spinosa, we highlight for the first time its potential applications in the foods. The findings will provide critical information for the future development of C. spinosa into a multifunctional food product with essential roles in health benefits.
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6
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Meurillon M, Anderson C, Angénieux M, Mercier F, Kondjoyan N, Engel E. Sensory acceptability of antioxidant-based formulations dedicated to mitigate heterocyclic aromatic amines in cooked meat. Meat Sci 2023; 198:109088. [PMID: 36623338 DOI: 10.1016/j.meatsci.2022.109088] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/08/2022] [Revised: 12/23/2022] [Accepted: 12/26/2022] [Indexed: 12/29/2022]
Abstract
In a previous work, we studied the inhibition of heterocyclic aromatic amine formation by natural ingredients rich in antioxidants: caper, oregano, wine and green tea. This present work aimed to assess the sensory impact of the addition of these ingredients in ground beef patties. The best liked formulations were determined by a hedonic scoring. Caper and oregano were not significantly different from the standard, the most appreciated, due to their congruency with cooked meat. Direct dissimilarity assessment was performed to evaluate the overall, gustative and odor differences between formulations. Olfactive differences were evidenced as key drivers of these differences. Standard and caper were close while oregano was found significantly different from them. A fast sensory profiling permitted to evidence that aromatic plant note was discriminant for oregano formulation. Gas chromatography-mass spectrometry/multibooth olfactometry identified the distinctive odor-active compounds of the formulations as pyrazines and sulfide for oregano formulation and ester for caper formulation.
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Affiliation(s)
- Maïa Meurillon
- INRAE, UR370 QuaPA, Microcontaminants, Aroma and Separation Science Lab, 63122 Saint-Genès-Champanelle, France.
| | - Chloé Anderson
- INRAE, UR370 QuaPA, Microcontaminants, Aroma and Separation Science Lab, 63122 Saint-Genès-Champanelle, France
| | - Magaly Angénieux
- INRAE, UR370 QuaPA, Microcontaminants, Aroma and Separation Science Lab, 63122 Saint-Genès-Champanelle, France
| | - Frédéric Mercier
- INRAE, UR370 QuaPA, Microcontaminants, Aroma and Separation Science Lab, 63122 Saint-Genès-Champanelle, France
| | - Nathalie Kondjoyan
- INRAE, UR370 QuaPA, Microcontaminants, Aroma and Separation Science Lab, 63122 Saint-Genès-Champanelle, France
| | - Erwan Engel
- INRAE, UR370 QuaPA, Microcontaminants, Aroma and Separation Science Lab, 63122 Saint-Genès-Champanelle, France
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7
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Zhang Z, Liang M, Liu B, Wu Y, Wang R, Liu Y. Characterization of the key odorants of ripe Capparis spinosa L. fruit and changes in concentrations after storage by means of molecular sensory science. Food Chem 2023; 403:134383. [DOI: 10.1016/j.foodchem.2022.134383] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/15/2022] [Revised: 09/18/2022] [Accepted: 09/20/2022] [Indexed: 11/16/2022]
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8
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The Current State of Knowledge about the Biological Activity of Different Parts of Capers. Nutrients 2023; 15:nu15030623. [PMID: 36771330 PMCID: PMC9919103 DOI: 10.3390/nu15030623] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/27/2022] [Revised: 01/17/2023] [Accepted: 01/22/2023] [Indexed: 01/27/2023] Open
Abstract
The caper, from the Latin capra, meaning goat, is the common name for the salt-fermented floral buds of the perennial shrubs of the Capparis genus (Capparacea family). This genus is represented by about 250 species, including the very popular C. spinosa L. While the whole plant is edible, the aromatic floral buds are most widely consumed, being collected by hand prior to blooming, dried in the sun and pickled. Capers are usually served marinated in vinegar, brine or oil. They have a significant potential as dietary supplements due to their low calorie content and richness in bioactive phytochemicals. Numerous in vitro and in vivo studies have demonstrated that C. spinosa have various nutritional and biological properties, including antioxidant activity resulting from the presence of phenolic compounds. The present paper reviews the current literature concerning the biological properties of the fruits, buds, seeds, roots and leaves of C. spinosa, including their toxicity.
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9
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Motti R, Paura B, Cozzolino A, de Falco B. Edible Flowers Used in Some Countries of the Mediterranean Basin: An Ethnobotanical Overview. PLANTS (BASEL, SWITZERLAND) 2022; 11:plants11233272. [PMID: 36501312 PMCID: PMC9736219 DOI: 10.3390/plants11233272] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/25/2022] [Revised: 11/15/2022] [Accepted: 11/21/2022] [Indexed: 05/14/2023]
Abstract
Edible flowers are becoming an essential component of people's nutrition in the Mediterranean basin. In the last decades, many researchers also have focused their attention on the nutritional composition of the edible flowers, as well as their antioxidant and antimicrobial properties, including studies on their safety issues. Despite the growing interest in the use of flowers in human nutrition, the ethnobotanical literature is lacking coverage of some important issues, particularly those which concern the use of flowers in the folk tradition. Only recently, a review regarding the contribution of 32 edible flowers to the Mediterranean diet was published. The aim of the present review is to document the plant lore regarding the wild and cultivated edible flowers consumed in the Mediterranean basin. Based on the 112 studies reviewed, we documented 251 taxa as being used in the Mediterranean basin as edible flowers. The plant species belong to 45 families and 141 genera. Asteraceae (54 taxa) is the most frequently cited family. Sambucus nigra L. is the most cited species. This study can be the basis for future research on the supposed bioactivity and toxicity of wild and cultivated flowers.
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Affiliation(s)
- Riccardo Motti
- Department of Agricultural Sciences, University of Naples Federico II, Via Università, 100 Portici, 80055 Naples, Italy
- Correspondence:
| | - Bruno Paura
- Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso, Italy
| | - Alessia Cozzolino
- Department of Agricultural Sciences, University of Naples Federico II, Via Università, 100 Portici, 80055 Naples, Italy
| | - Bruna de Falco
- Spanish Bank of Algae, Marine Biotechnology Center, University of Las Palmas de Gran Canaria, Muelle de Taliarte s/n, 35214 Telde, Spain
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10
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Cincotta F, Merlino M, Verzera A, Gugliandolo E, Condurso C. Innovative Process for Dried Caper ( Capparis spinosa L.) Powder Production. Foods 2022; 11:foods11233765. [PMID: 36496573 PMCID: PMC9740950 DOI: 10.3390/foods11233765] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/10/2022] [Revised: 10/20/2022] [Accepted: 11/21/2022] [Indexed: 11/24/2022] Open
Abstract
This research aimed to develop a new time, energy, and cost-saving production process for obtaining dried powder from Capparis spinosa floral buds. Four different trials, including dry salting with 40% NaCl (for 10 days and 40 days) and brine salting with 18% NaCl (at room temperature for 3 days and at 60 °C for 6 h), were carried out, and two different air-drying temperatures (40 and 50 °C) were used. The effects on chemical and sensory characteristics were investigated and compared with traditional undried caper samples. Spectroscopy and chromatographic techniques such as UV-VIS, GC-MS, and FTIR were used for chlorophylls, carotenoids, polyphenols, flavonoids, and volatile aroma compounds' analyses. Moreover, a sensory descriptive analysis and acceptability were applied to individuate the product most appreciated by the consumers. Among the different trials, brine salting at 60 °C and drying at 50 °C constituted the fastest process that yielded an appreciated powder by consumers; the chemical analyses demonstrated that this process did not lead to the formation of extraneous aroma compounds that could influence the typical sensory properties of capers and maintained high levels of chlorophylls, carotenoids, and polyphenols. Altogether, the results could be of great significance to industrial production and potentiate positive impacts on the economy of production areas.
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11
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Annaz H, Sane Y, Bitchagno GTM, Ben Bakrim W, Drissi B, Mahdi I, El Bouhssini M, Sobeh M. Caper (Capparis spinosa L.): An Updated Review on Its Phytochemistry, Nutritional Value, Traditional Uses, and Therapeutic Potential. Front Pharmacol 2022; 13:878749. [PMID: 35935860 PMCID: PMC9353632 DOI: 10.3389/fphar.2022.878749] [Citation(s) in RCA: 29] [Impact Index Per Article: 9.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/18/2022] [Accepted: 05/25/2022] [Indexed: 11/13/2022] Open
Abstract
Caper (Capparis spinosa L.) is a perennial shrub of the family Capparaceae, endemic to circum-Mediterranean countries. Caper carries a renowned nutritional value, especially in terms of vitamins and antioxidants related to the occurrence of flavonoids, alkaloids, and glucosinolates as main secondary metabolites. Caper extracts have also shown to display antibacterial, antifungal, analgesic, antitumor, hepatoprotective, antioxidant, anti-inflammatory, and neuroprotective effects which correlate the uses of the plant in folk medicine against both metabolic and infectious diseases. The present review aims to provide exhaustive phytochemistry and pharmacological properties survey on Caper constituents. Attention has also been given to the nutritional values and traditional uses of main organs to pinpoint research gaps for future investigations on the plant.
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Affiliation(s)
- Hassan Annaz
- AgrobioSciences Research, Mohammed VI Polytechnic University, Ben-Guerir, Morocco
- *Correspondence: Hassan Annaz, ; Mansour Sobeh,
| | - Yaya Sane
- AgrobioSciences Research, Mohammed VI Polytechnic University, Ben-Guerir, Morocco
| | | | - Widad Ben Bakrim
- AgrobioSciences Research, Mohammed VI Polytechnic University, Ben-Guerir, Morocco
- African Sustainable Agriculture Research Institute (ASARI), Mohammed VI Polytechnic University (UM6P), Laayoune, Morocco
| | - Badreddine Drissi
- AgrobioSciences Research, Mohammed VI Polytechnic University, Ben-Guerir, Morocco
| | - Ismail Mahdi
- AgrobioSciences Research, Mohammed VI Polytechnic University, Ben-Guerir, Morocco
| | | | - Mansour Sobeh
- AgrobioSciences Research, Mohammed VI Polytechnic University, Ben-Guerir, Morocco
- *Correspondence: Hassan Annaz, ; Mansour Sobeh,
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12
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Liu H, Chang G, Wang W, Ji Z, Cui J, Peng Y. Pharmacokinetics, Prostate Distribution and Metabolic Characteristics of Four Representative Flavones after Oral Administration of the Aerial Part of Glycyrrhiza uralensis in Rats. Molecules 2022; 27:molecules27103245. [PMID: 35630722 PMCID: PMC9144537 DOI: 10.3390/molecules27103245] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/11/2022] [Revised: 05/11/2022] [Accepted: 05/12/2022] [Indexed: 11/16/2022] Open
Abstract
(1) Background: The aerial part of G. uralensis had pharmacological effects against chronic non-bacterial prostatitis (CNP), and flavonoids are the main efficacy components. The purpose of this study was to obtain the pharmacokinetics, prostate distribution and metabolic characteristics of some flavonoids in rats. (2) Methods: The prototype flavones and the metabolites of four representative flavonoids, namely puerarin, luteolin, kaempferol and pinocembrin in plasma, prostate, urine and feces of rats were analyzed by UPLC-Q-Exactive Orbitrap-MS. In addition, the pharmacokinetic parameters in plasma and distribution of prostate of four components were analyzed by HPLC-MS/MS. (3) Results: In total, 22, 17, 22 and 11 prototype flavones were detected in the prostate, plasma, urine and feces, respectively. The metabolites of puerarin in the prostate are hydrolysis and glucose-conjugated products, the metabolites of kaempferol and luteolin in the prostate are methylation and glucuronidation, and the metabolites of pinocembrin in the prostate are naringenin, oxidation, sulfation, methylation and glucuronidation products. The t1/2 of puerarin, luteolin, kaempferol and pinocembrin was 6.43 ± 0.20, 31.08 ± 1.17, 18.98 ± 1.46 and 13.18 ± 0.72 h, respectively. The concentrations of the four flavonoids in prostate were ranked as kaempferol > pinocembrin > luteolin > puerarin. (4) Conclusions: Methylation and glucuronidation metabolites were the main metabolites detected in the prostate. A sensitive and validated HPLC−MS/MS method for simultaneous determination of puerarin, luteolin, kaempferol and pinocembrin in rat plasma and prostate was described, and it was successfully applied to the pharmacokinetic and prostate distribution studies.
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Affiliation(s)
- Haifan Liu
- Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; (H.L.); (J.C.)
| | - Guanhua Chang
- Beijing Wehand-Bio Pharmaceutical Co., Ltd., Beijing 102629, China;
| | - Wenquan Wang
- Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; (H.L.); (J.C.)
- Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs, Ministry of Education, Beijing 100102, China
- Correspondence: (W.W.); (Z.J.); Tel.: +86-15811588577 (W.W.); +86-13999841009 (Z.J.)
| | - Zuen Ji
- Xinjiang Key Laboratory for Reserch of Licorice and Products, Korla 841011, China;
- Xinjiang Quanan Pharmaceutical Co., Ltd., Korla 841011, China
- Correspondence: (W.W.); (Z.J.); Tel.: +86-15811588577 (W.W.); +86-13999841009 (Z.J.)
| | - Jie Cui
- Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; (H.L.); (J.C.)
| | - Yifeng Peng
- Xinjiang Key Laboratory for Reserch of Licorice and Products, Korla 841011, China;
- Xinjiang Quanan Pharmaceutical Co., Ltd., Korla 841011, China
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13
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Liu Z, Fang Y, Wu C, Hai X, Xu B, Li Z, Song P, Wang H, Chao Z. The Difference of Volatile Compounds in Female and Male Buds of Herpetospermum pedunculosum Based on HS-SPME-GC-MS and Multivariate Statistical Analysis. Molecules 2022; 27:molecules27041288. [PMID: 35209076 PMCID: PMC8879731 DOI: 10.3390/molecules27041288] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/23/2022] [Revised: 02/09/2022] [Accepted: 02/11/2022] [Indexed: 12/10/2022] Open
Abstract
Herpetospermum pedunculosum (Ser.) C. B. Clarke (Family Cucurbitaceae) is a dioecious plant and has been used as a traditional Tibetan medicine for the treatment of hepatobiliary diseases. The component, content, and difference in volatile compounds in the female and male buds of H. pedunculosum were explored by using headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) technology and multivariate statistical analysis. The results showed that isoamyl alcohol was the main compound in both female and male buds and its content in males was higher than that in females; 18 compounds were identified in female buds including 6 unique compounds such as (E)-4-hexenol and isoamyl acetate, and 32 compounds were identified in male buds, including 20 unique compounds such as (Z)-3-methylbutyraldehyde oxime and benzyl alcohol. (Z)-3-methylbutyraldehyde oxime and (E)-3-methylbutyraldehyde oxime were found in male buds, which only occurred in night-flowering plants. In total, 9 differential volatile compounds between female and male buds were screened out, including isoamyl alcohol, (Z)-3-methylbutanal oxime, and 1-nitropentane based on multivariate statistical analysis such as principal component analysis (PCA) and orthogonal partial least squares discrimination analysis (OPLS-DA). This is the first time to report the volatile components of H. pedunculosum, which not only find characteristic difference between female and male buds, but also point out the correlation between volatile compounds, floral odor, and plant physiology. This study enriches the basic theory of dioecious plants and has guiding significance for the production and development of H. pedunculosum germplasm resources.
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Affiliation(s)
- Zhenying Liu
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; (Z.L.); (C.W.); (B.X.); (Z.L.); (P.S.); (H.W.)
| | - Ye Fang
- Shangri-La Alpine Botanical Garden, Diqing 674400, China; (Y.F.); (X.H.)
| | - Cui Wu
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; (Z.L.); (C.W.); (B.X.); (Z.L.); (P.S.); (H.W.)
| | - Xian Hai
- Shangri-La Alpine Botanical Garden, Diqing 674400, China; (Y.F.); (X.H.)
| | - Bo Xu
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; (Z.L.); (C.W.); (B.X.); (Z.L.); (P.S.); (H.W.)
| | - Zhuojun Li
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; (Z.L.); (C.W.); (B.X.); (Z.L.); (P.S.); (H.W.)
| | - Pingping Song
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; (Z.L.); (C.W.); (B.X.); (Z.L.); (P.S.); (H.W.)
| | - Huijun Wang
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; (Z.L.); (C.W.); (B.X.); (Z.L.); (P.S.); (H.W.)
| | - Zhimao Chao
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; (Z.L.); (C.W.); (B.X.); (Z.L.); (P.S.); (H.W.)
- Correspondence: or ; Tel.: +86-135-2270-5161
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Capparis spinosa Alleviates DSS-Induced Ulcerative Colitis via Regulation of the Gut Microbiota and Oxidative Stress. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2021; 2021:1227876. [PMID: 34956375 PMCID: PMC8695000 DOI: 10.1155/2021/1227876] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 08/07/2021] [Revised: 10/15/2021] [Accepted: 10/19/2021] [Indexed: 01/11/2023]
Abstract
Ulcerative colitis (UC) is a chronic inflammatory disease. Here, the potential effects of Capparis spinosa water extract (CSWE) on colonic histopathology, inflammation, and gut microbiota composition in dextran sulfate sodium (DSS) induced UC mice were evaluated. Our results showed that CSWE treatment improved the colonic histopathology of UC mice, increased the levels of tight junction protein gene ZO-1 and Occludin in intestinal epithelial cells, and inhibited the expression of proinflammatory cytokines (IL-1β, IL-6, and TNF-α). Furthermore, CSWE administration alleviated oxidative stress in the colon of UC mice. The effects of CSWE on the compositions and metabolomic profiles of the gut microbiota in UC mice were investigated. It was found that CSWE could enhance the diversity of gut microbes and the abundance of probiotics and metabonomics had the strongest association with Firmicutes. Our results indicated that CSWE might be an ideal candidate as a potential therapeutic natural product for the treatment of UC.
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15
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Gou M, Bi J, Chen Q, Wu X, Fauconnier ML, Qiao Y. Advances and Perspectives in Fruits and Vegetables Flavor Based on Molecular Sensory Science. FOOD REVIEWS INTERNATIONAL 2021. [DOI: 10.1080/87559129.2021.2005088] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/01/2023]
Affiliation(s)
- Min Gou
- Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (Caas)/ Key Laboratory of Agro-Products Processing, Beijing, China
- Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, University of Liege, Gembloux Belgium
| | - Jinfeng Bi
- Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (Caas)/ Key Laboratory of Agro-Products Processing, Beijing, China
| | - Qinqin Chen
- Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (Caas)/ Key Laboratory of Agro-Products Processing, Beijing, China
| | - Xinye Wu
- Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (Caas)/ Key Laboratory of Agro-Products Processing, Beijing, China
| | - Marie-Laure Fauconnier
- Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, University of Liege, Gembloux Belgium
| | - Yening Qiao
- Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (Caas)/ Key Laboratory of Agro-Products Processing, Beijing, China
- Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, University of Liege, Gembloux Belgium
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16
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Zhu X, Yang Y, Gao W, Jiang B, Shi L. Capparis spinosa Alleviates DSS-Induced Ulcerative Colitis via Regulation of the Gut Microbiota and Oxidative Stress. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE : ECAM 2021. [PMID: 34956375 DOI: 10.1002/10.1155/2021/1227876] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Subscribe] [Scholar Register] [Indexed: 04/29/2023]
Abstract
Ulcerative colitis (UC) is a chronic inflammatory disease. Here, the potential effects of Capparis spinosa water extract (CSWE) on colonic histopathology, inflammation, and gut microbiota composition in dextran sulfate sodium (DSS) induced UC mice were evaluated. Our results showed that CSWE treatment improved the colonic histopathology of UC mice, increased the levels of tight junction protein gene ZO-1 and Occludin in intestinal epithelial cells, and inhibited the expression of proinflammatory cytokines (IL-1β, IL-6, and TNF-α). Furthermore, CSWE administration alleviated oxidative stress in the colon of UC mice. The effects of CSWE on the compositions and metabolomic profiles of the gut microbiota in UC mice were investigated. It was found that CSWE could enhance the diversity of gut microbes and the abundance of probiotics and metabonomics had the strongest association with Firmicutes. Our results indicated that CSWE might be an ideal candidate as a potential therapeutic natural product for the treatment of UC.
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Affiliation(s)
- Xiaoting Zhu
- College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
| | - Yi Yang
- College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
| | - Weizhen Gao
- College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
| | - Bingjie Jiang
- College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
| | - Lei Shi
- College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
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17
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Aksay O, Selli S, Kelebek H. LC-DAD-ESI-MS/MS-based assessment of the bioactive compounds in fresh and fermented caper (Capparis spinosa) buds and berries. Food Chem 2020; 337:127959. [PMID: 32916535 DOI: 10.1016/j.foodchem.2020.127959] [Citation(s) in RCA: 21] [Impact Index Per Article: 4.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/31/2020] [Revised: 08/22/2020] [Accepted: 08/28/2020] [Indexed: 01/22/2023]
Abstract
The main objective of this study was to compare bioactive compounds and other important quality parameters of fresh and fermented caper buds and berries. Fresh samples were fermented using dry-salted and brined techniques. The higher phenolic content was determined in the fresh (1843.71 mg/100 g DW) and fermented buds (1198.54-1539.49 mg/100 g DW) rather than the berries (29.72-40.75 mg/100 g DW). Quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, and quercetin-O-galloly-O-hexoside were the principal phenolic components in fresh and fermented buds while quercetin-3-O-rutinoside in fresh and fermented berries. The amounts of isorhamnetin, quercetin, and kaempferol increased in fermented buds and berries compared to fresh samples. Similarly, antioxidant capacity of buds was found to be markedly higher than berries. As for sugar compounds, it was found that fructose in buds (1.56-3.23 g/100 g DW) and glucose in berries (1.96-6.38 g/100 g DW) had the highest amount. When total phenolics and antioxidant properties were evaluated, it was observed that they were better preserved in the dry-salted samples than the brined samples.
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Affiliation(s)
- Ozge Aksay
- Department of Food Engineering, Faculty of Engineering, Adana Alparslan Turkes Science and Technology University, Adana, Turkey
| | - Serkan Selli
- Department of Food Engineering, Faculty of Agriculture, Cukurova University, 01330 Adana, Turkey
| | - Hasim Kelebek
- Department of Food Engineering, Faculty of Engineering, Adana Alparslan Turkes Science and Technology University, Adana, Turkey.
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18
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Zannou O, Kelebek H, Selli S. Elucidation of key odorants in Beninese Roselle (Hibiscus sabdariffa L.) infusions prepared by hot and cold brewing. Food Res Int 2020; 133:109133. [DOI: 10.1016/j.foodres.2020.109133] [Citation(s) in RCA: 20] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/04/2019] [Revised: 02/25/2020] [Accepted: 02/26/2020] [Indexed: 11/25/2022]
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