Copyright
©The Author(s) 2020.
World J Clin Infect Dis. May 15, 2020; 10(1): 14-23
Published online May 15, 2020. doi: 10.5495/wjcid.v10.i1.14
Published online May 15, 2020. doi: 10.5495/wjcid.v10.i1.14
Strain/plasmid | Description | Source |
Strain | ||
DH5a MCR | Transformation efficient strain | Gibco/BRL |
MC4100 | E. coli K-12 strain | Linda Kenney |
EK227 | E. coli K-12 strain | [53] |
EF1007 | gadE::Km in EK227 | [54] |
EF1083 | gadE::Km/pPCRScript Amp gadE | [33] |
NU149 | Clinical isolate | [36] |
NU149 gadE | ΔgadE mutation in NU149 | This study |
NU149 LacZ1 | ΔlacZ mutation in NU149 | This study |
Plasmid | ||
pUJ9 | Promoterless lacZ gene, ApR | [37] |
pPP2-6 | Single copy plasmid, CmR | [38] |
pKD4 | Flp recombinase sites, KmR | [40] |
pKD46 | Red recombinase, ApR | [40] |
pCP20 | Flp recombinase, ApR | [40] |
pNK2-29 | fliC::lacZ on pPP2-6, ApR | This study |
pPCRScript Amp gadE | gadE on pPCRScript Amp | [33] |
pH | Gal activity1 |
5.5 | 288 ± 81.5 |
6 | 528 ± 82.5 |
6.5 | 629 ± 114 |
7 | 1111 ± 110 |
7.5 | 932 ± 190 |
8 | 738 ± 125 |
E. coli strain | NaCl (mmol/L) | Gal activity1 | |
pH 5.5 | pH 7.0 | ||
MC4100/pNK2-29 | 0 | 308 ± 1042 | 1132 ± 130 |
MC4100/pNK2-29 | 100 | 338 ± 128 | 806 ± 41 |
MC4100/pNK2-29 | 200 | 251 ± 68.5 | 689 ± 173 |
MC4100/pNK2-29 | 400 | 62 ± 22.0 | 454 ± 71 |
NU149 LacZ1/pNK2-29 | 0 | 442 ± 72 | 1353 ± 98 |
NU149 LacZ1/pNK2-29 | 100 | 418 ± 61 | 976 ± 52 |
NU149 LacZ1/pNK2-29 | 200 | 293 ± 43 | 811 ± 75 |
NU149 LacZ1/pNK2-29 | 400 | 147 ± 39 | 489 ± 61 |
- Citation: Schwan WR, Flohr NL, Multerer AR, Starkey JC. GadE regulates fliC gene transcription and motility in Escherichia coli. World J Clin Infect Dis 2020; 10(1): 14-23
- URL: https://www.wjgnet.com/2220-3176/full/v10/i1/14.htm
- DOI: https://dx.doi.org/10.5495/wjcid.v10.i1.14