Establishment and characterization of a new human gallbladder cancer cell line, OCUG-2

Table 1 Short tandem repeat analysis

Microsatellite (chromosome number)	OCUG-2 cells
D3S1358 (3)	16
TH01 (11)	7, 9
D21S11 (21)	30
D18S51 (18)	13
Penta E (15)	12
D5S818 (5)	12
D13S317 (13)	12
D7S820 (7)	9, 12
D16S539 (16)	9, 10
CSF1PO (5)	12
Penta D (21)	11
Amelogenin (X Y)	X
vWA (12)	16
D8S1179 (8)	13, 15
TPOX (2)	8, 12
FGA (4)	20, 21

Table 2 Correlation between clinicopathologic features and insulin-like growth factor 1 receptor expression in gallbladder cancer

Feature	IGF1R-positive (n = 17)	IGF1R-negative (n = 17)	P value
Sex
Male	6	10
Female	11	7	0.17
Chemotherapy
Yes	11	10
No	6	7	0.72
T stage
T1/T2	8	10
T3/T4	9	7	0.49
N stage
Negative	8	12
Positive	9	5	0.16
M stage
Negative	14	16
Positive	3	1	0.28
Clinical stage
I/II	6	10
III/IV	11	7	0.16

IGF1R: Insulin-like growth factor 1 receptor.

Table 3 Univariate and multivariate regression analyses of overall survival

Feature	Univariate analysis		Multivariate analysis
	Hazard ratio (95%CI)	P value	Hazard ratio (95%CI)	P value
Sex	0.658 (0.259-1.672)	NS
Chemotherapy	3.039 (1.077-8.578)	0.03	1.969 (0.594-6.517)	NS
T	1.771 (1.165-2.692)	0.005
N	2.833 (1.2-6.687)	0.01
M	4.905 (1.516-15.88)	0.003
Stage	1.767 (1.146-2.723)	0.006	1.595 (0.950-2.679)	0.077
IGF1R expression	2.654 (1.102-6.913)	0.04	3.302 (1.208-9.023)	0.02

CI: Confidence interval; IGF1R: Insulin-like growth factor 1 receptor; NS: Not significant.

Table 4 Candidate gene fusions detected in RNA sequencing data of OCUG-2 cells

Gene fusion	Breakpoint1	Breakpoint2	Confidence	Reading frame
LEPROT: LEPR	chr1:65425378	chr1:65565546	High	Out-of-frame
LEPROT: LEPR	chr1:65430048	chr1:65565546	Medium	Out-of-frame
TSPAN14: AL392185.1	chr10:80504778	chr9:95602159	High	Out-of-frame
TSPAN14: AL392185.1	chr10:80489314	chr9:95602159	High	Out-of-frame
TSPAN14: AL392185.1	chr10:80506862	chr9:95602874	Medium
TSPAN14: AL392185.1	chr10:80504778	chr9:95600165	Medium	Out-of-frame
RNF115: ITGA10	chr1:145823772	chr1:145907465	High	Out-of-frame
PCDHA3: PCDHAC2	chr5:140803591	chr5:140978949	High	In-frame
AC092807.3: DDAH1	chr1:85496166	chr1:85358847	High
AC092807.3: DDAH1	chr1:85494729	chr1:85358847	Medium
AC092807.3: DDAH1	chr1:85577984	chr1:85358847	Medium
ERC1: PDE3A	chr12:1141787	chr12:20556660	High	In-frame
TVP23C: CDRT4	chr17:15540433	chr17:15440285	High	Out-of-frame
PRR16: AC114284.1	chr5:120464645	chr5:120789954	High	Out-of-frame
PCDHGB2: PCDHGA8	chr5:141362556	chr5:141494807	Medium	In-frame
MYO9A: PKM	chr15:72117680	chr15:72219110	Medium
RASSF8: SSPN	chr12:26055446	chr12:26224293	Medium	Out-of-frame
RASSF8: SSPN	chr12:26055446	chr12:26179996	Medium	Out-of-frame
AC104123.1: CAST	chr5:96379652	chr5:96675539	Medium
MGST1: AC007529.2	chr12:16383604	chr12:16661766	Medium
LDLRAD3: PRR5L	chr11:36098461	chr11:36296290	Medium	Out-of-frame