Chemical profiling of bioactive compounds in the methanolic extract of wild leaf and callus of Vitex negundo using gas chromatography-mass spectrometry

Table 1 Effect of combinations of Plant Genetic Resources in mass spectrometry medium on callus induction in Vitex negundo

MS + hormone (mg/L)	Medium name	Source of explants	Number of explants inoculated	Callus response (%)	Callus genesis	Callus initiation (time/d)	Color	Texture
MS + no hormone	M1	Leaf	13	-	-	-	-	-
MS + BAP (2.0) + 2,4D (0.2)	M2	Leaf	13	15 d	+++	100%	Green	Friable
MS + BAP (2.0) + NAA (0.2)	M3	Leaf	13	15 d	++	70%	Greenish white	Friable
MS + DPU (2.0) + 2,4D (0.2)	M4	Leaf	13	15 d	+++	90%	White-yellowish	Loose
MS + DPU (2.0) + NAA (0.2)	M5	Leaf	13	15 d	+	40%	White-yellowish	Loose

MS: Mass spectrometry; BAP: 6-benzylaminopurine; NAA: α-naphthylacetic acid; DPU: Di-phenylurea.

Table 2 Bioactive components detected in the wild leaves methanol extract of Vitex negundo (L.)

Peak	Retention time	Area (%)	Name of components
1	6.330	1.12	Dimethylphosphine-D1
2	6.989	0.72	2-[2-hydroxyethyl]-9-[beta-d-ribofuranosyl] hypoxanthine
3	7.304	1.31	7-tetradecenyl-1-(N-acetyl)amine
4	9.655	0.56	1-propanol, 3-mercapto-sulfanyl-propanol
5	13.301	3.62	3-methylpyridazine pyridazine
6	14.458	0.55	2-octyldodecan-1-ol
7	14.992	3.96	3-hydroxyphenylacetylene 3-ethynylphenol
8	16.003	0.59	Z-citral 2,6-Octadienal, 3,7-dimethyl-, (Z)- (CAS)
9	16.559	03.80	1H-indenol
10	17.028	0.82	2-fluorobenzyl alcohol, benzene-methanol
11	17.994	4.28	Benzene, 1-methoxy-4-enylanisole
12	18.543	0.91	N-hexadecylpyridinium bromide
13	20.132	0.90	9-chloro-8-oxatetracyclotridecan
14	20.315	0.58	5-chlorovaleric acid, octyl ester
15	21.934	1.09	Dimethyl-2-phenyl-2-propenyl phosphite
16	23.156	0.92	4-chromanol-benzopyran-4
17	23.925	6.79	D-viridiflorol
18	24.379	0.75	8,9-epoxy-6,6-dimethyl-3,4-undecadien
19	24.416	1.51	Drimenol
20	28.421	7.91	1-oxo-5,5-dimethyl-6-methylene-hexahydrocyclopentano pyran
21	29.761	6.79	Pyrrolo-carbazole (CAS)
22	30.698	6.74	Dimethyl (phenyl) silane
23	34.044	21.93	9,12-octadecadienoic acid
24	34.476	21.84	2-hexadecen-1-OL, TETRAM

Table 3 Bioactive components detected in the green callus methanol extract of Vitex negundo (L.)

Peak	Retention time	Area (%)	Name of components
1	6.147	2.60	N-butyric-D7 acid
2	9.523	1.50	Benzyloxy-6-tetrabutyldimethyldimethylsilyl-oxy-methyl
3	18.002	1.76	Benzene, 1-methoxy-4 (CAS) anethole-p propenyl isole
4	24.833	1.23	Tridecanedial
5	29.373	1.50	Nor sesterterpene diene ester
6	29.497	1.38	But-2-enyliden-6-tert-butyl-7-methyl-tetrahydrocy
7	30.339	3.77	Hexadecanoic acid, methyl ester
8	31.950	1.63	Tricyclo-undecan-10-imine
9	32.382	36.82	Dursban
10	34.220	47.79	10,13-octadecadienoic acid, methyl ester

Table 4 Bioactive components detected in the white loose callus of methanol extract of Vitex negundo (L.)

Peak	Retention time	Area (%)	Name of components
1	6.016	0.58	Acetoxy-beta-caryophyllene
2	6.177	1.43	Anhalonine
3	9.552	0.84	1,3,5-cycloheptatriene-cyclohep-triene-cycloheptatrien
4	17.943	0.46	2,3-dichloropyridine 2, 3-dichloropyridine
5	18.024	0.83	1-methoxy-4-propenyl benzene
6	23.361	0.80	6,7-dimethyl-triazolo-triazine
7	24.914	0.51	Benzene, 2-methylthio-ethenyl
8	28.670	0.63	Spiro-decan-7-one, 1,8-dimethy epoxy-4-isopropyl
9	29.351	3.08	Methyl-cyclohex-1-enyl
10	30.347	6.31	14-pentadecynoic acid, methyl ester (CAS)
11	31.613	0.55	2,5-furandione, di hydro-dodecenylsuccinic anhydrid
12	32.338	42.98	Chlorpyrifos
13	34.154	40.38	Octadecadienoic acid, methyl ester
14	39.982	0.61	8-alpha,12-epoxy-hexanorlabdane