Cytoplasmic and nuclear programmed death ligand 1 expression in peritumoral stromal cells in breast cancer: Prognostic and predictive value

Figure 1 Assessment of the programmed cell death protein 1 ligand 1-positive LF score. A: Relative density of immune cells (ICs) – 2; percentage of programmed cell death protein 1 ligand 1-positive (PDCD1 LG1+) ICs - 1. PDCD1 LG1+ LF score = 2 (1 × 2); B: Relative density of ICs - 2; percentage of PDCD1 LG1+ ICs - 1. PDCD1 LG1+ LF score = 2 (2 × 1); C: Relative density of ICs - 3; percentage of PDCD1 LG1+ ICs - 2. The PDCD1 LG1+ LF score = 6 (3 × 2); D: Relative density of ICs - 3; percentage of PDCD1 LG1+ ICs - 4. PDCD1 LG1+ LF score = 12 (3 × 4). Immunohistochemistry staining with antibodies against PDCD1 LG1, 800 ×.

Figure 2 Nuclear expression of programmed cell death protein 1 ligand 1 in lymphocytes of the peritumoral stroma. A: Absence of nuclear programmed cell death protein 1 ligand 1 (PDCD1 LG1) expression in lymphocytes; B: Presence of nuclear PDCD1 LG1 expression in lymphocytes. Immunohistochemistry staining with antibodies against PDCD1 LG1, 800 ×.

Figure 3 The severity of programmed cell death protein 1 ligand 1 expression in polymorphic cell infiltration. A: No polymorphic cell infiltration; B: Weak programmed cell death protein 1 ligand 1-positive (PDCD1 LG1+) polymorphic cell infiltration; C: Moderate PDCD1 LG1+ polymorphic cell infiltration; D: Pronounced expressed PDCD1 LG1+ polymorphic cell infiltration. Immunohistochemistry staining with antibodies against PDCD1 LG1, 200 ×.

Figure 4 Presence of programmed cell death protein 1 ligand 1-positive fibroblastic stroma. A and B: Absence of programmed cell death protein 1 ligand 1-positive (PDCD1 LG1+) fibroblastic stroma; C and D: Presence of PDCD1 LG1+ fibroblastic stroma. Immunohistochemistry staining with antibodies against PDCD1 LG1. Magnification: A and C: 200 ×; B and D: 800 ×.

Figure 5 Presence of programmed cell death protein 1 ligand 1 expression in tumor microvessels. A: Vessel without programmed cell death protein 1 ligand 1 (PDCD1 LG1) expression; B: Vessels with PDCD1 LG1 expression, immunohistochemistry staining with antibodies against PDCD1 LG1, 800 ×.

Figure 6 Assessment of the SP142+ immune cell score. А: SP142+ IC0 (< 1%); В: SP142+ IC1 (≥ 1%), immunohistochemistry staining with antibodies against SP142, 200 ×.