• biologic agents
• morbidity
• pain
• photobiomodulation
• tissue grafts
• treatment errors

• Humans
• Palate/surgery
• Wound Healing
• Plastic Surgery Procedures
• Morbidity
• Tissue and Organ Harvesting/adverse effects

• Injectable platelet-rich fibrin
• Palatal tissue healing
• Periodontal surgery
• Subepithelial connective tissue graft Tissue thickness.

• Laser
• Low-level laser therapy
• Palate
• Platelet-rich fibrin
• VIP-CT flap

• Humans
• Platelet-Rich Fibrin
• Low-Level Light Therapy
• Wound Healing
• Connective Tissue
• Pain

• pain
• palate
• sutures
• tissue adhesives
• wound healing

Collagen membrane and platelet-rich fibrin (PRF) have emerged as vital biomaterials in the field of periodontal regeneration. Minimally invasive techniques are being preferred by most periodontists, as it is patient compliant with fewer post-surgical complications as compared to conventional surgical techniques. Thus, in this study we have evaluated the effect of injectable PRF (i-PRF) with collagen membrane compared with collagen membrane alone using vestibular incision subperiosteal tunnel access (VISTA) technique for gingival recession coverage.

To compare the efficacy of VISTA using collagen membrane with collagen membrane soaked in injectable PRF for gingival recession coverage.

A split mouth randomized controlled clinical trial was designed;13 subjects having at least 2 teeth indicated for recession coverage were enrolled in this study. The sites were randomly assigned to control group (VISTA using collagen membrane alone) and the test group (VISTA using collagen membrane with i-PRF). The clinical parameters assessed were pocket depth, recession depth (RD), recession width (RW), relative attachment level, keratinised tissue width (KTW), keratinised tissue thickness (KTT), and percentage root coverage.

RD showed a statistically significant difference between the test group at 3 mo (0.5 ± 0.513) and 6 mo (0.9 ± 0.641) and the control group at 3 mo (0.95 ± 0.51) and 6 mo (1.5 ± 0.571), with P values of 0.008 and 0.04, respectively. RW also showed a statistically significant difference between the test group at 3 mo (1 ± 1.026) and 6 mo (1.65 ± 1.04) and the control group at 3 mo (1.85 ± 0.875) and 6 mo (2.25 ± 0.759), with P values of 0.008 and 0.001, respectively. Results for KTW showed statistically significant results between the test group at 1 mo (2.85 ± 0.489), 3 mo (3.5 ± 0.513), and 6 mo (3.4 ± 0.598) and the control group at 1 mo (2.45 ± 0.605), 3 mo (2.9 ± 0.447), and 6 mo (2.75 ± 0.444), with P values of 0.04, 0.004, and 0.003, respectively. Results for KTT also showed statistically significant results between test group at 1 mo (2.69 ± 0.233), 3 mo (2.53 ± 0.212), and 6 mo (2.46 ± 0.252) and the control group at 1 mo (2.12 ± 0.193), 3 mo (2.02 ± 0.18), and 6 mo (1.91 ± 0.166), with P values of 0.001, 0.001, and 0.001, respectively. The test group showed 91.6%, 81.6%, and 67% root coverage at 1 mo, 3 mo, and 6 mo, while the control group showed 82.3%, 66.4%, and 53.95% of root coverage at 1 mo, 3 mo, and 6 mo, respectively.

The use of minimally invasive VISTA technique along with collagen membrane and injectable form of platelet-rich fibrin can be successfully used as a treatment method for multiple or isolated gingival recessions of Miller’s class-I and class-II defects.

• Vestibular incision subperiosteal tunnel access
• Injectable platelet-rich fibrin
• Collagen membrane
• Gingival recessions
• Treatment

The first bioprocess that occurs in response to wounding is the deterrence of local hemorrhage. This is accomplished by platelet aggregation and initiation of the hemostasis cascade. The resulting blood clot immediately enables the cessation of bleeding and then functions as a provisional matrix for wound healing, which begins a few days after injury. Here, fibrinogen and fibrin fibers are the key players, because they literally serve as scaffolds for tissue regeneration and promote the migration of cells, as well as the ingrowth of tissues. Fibrin is also an important modulator of healing and a host defense system against microbes that effectively maintains incoming leukocytes and acts as reservoir for growth factors. This review presents recent advances in the understanding and applications of fibrin and fibrin-fiber-incorporated biomedical materials applied to wound healing and subsequent tissue repair. It also discusses how fibrin-based materials function through several wound healing stages including physical barrier formation, the entrapment of bacteria, drug and cell delivery, and eventual degradation. Pure fibrin is not mechanically strong and stable enough to act as a singular wound repair material. To alleviate this problem, this paper will demonstrate recent advances in the modification of fibrin with next-generation materials exhibiting enhanced stability and medical efficacy, along with a detailed look at the mechanical properties of fibrin and fibrin-laden materials. Specifically, fibrin-based nanocomposites and their role in wound repair, sustained drug release, cell delivery to wound sites, skin reconstruction, and biomedical applications of drug-loaded fibrin-based materials will be demonstrated and discussed.

Our study compared the effects of injectable platelet-rich fibrin (i-PRF) with those of corticosteroids in the treatment of erosive oral lichen planus (EOLP).

This split-mouth study included 24 individuals diagnosed histopathologically with bilateral EOLP. One bilateral lesion was injected with i-PRF, whereas the other was injected with methylprednisolone acetate in four sessions at 15-day intervals. Visual analog scale (VAS) for pain and satisfaction, oral health impact profile scale-14, and the lesion size were used.

The intragroup comparisons showed a significant decrease in VAS-pain and lesion size in both the i-PRF group (from 81.88±17.74 to 13.33±18.34, and from 4.79±0.41 to 1.88±1.08, respectively) and the corticosteroid group (from 80.21±17.35 to 23.33±26.81, and from 4.71±0.46 to 2.21±1.35, respectively) in the 6th month compared to baseline (p<0.001). Moreover, VAS-satisfaction increased significantly in both the i-PRF group (from 26.67±17.8 to 85.63±16.24) and the corticosteroid group (from 28.33±17.05 to 74.38±24.11) in the 6th month compared to baseline (p<0.001). However, no significant difference in any value occurred in the intergroup comparisons.

In patients with EOLP, both methods decreased pain and lesion size similarly, and both increased satisfaction. Therefore, the use of i-PRF may be considered an option in cases refractory to topical corticosteroid therapy. Biochemical and histopathological studies are required to reveal the mechanism of i-PRF action in EOLP treatment.

Platelet-rich fibrin (PRF) is a biomaterial that promotes wound healing. It has a fibrinous matrix wherein platelets, pro-inflammatory cytokines, and various growth factors along with few cells are entrapped while Chitosan is a naturally occurring cationic biopolymeric material that is derived from an animal product, chitin. It has demonstrated biological properties which include acceleration in wound healing, hemostasis, enhancement of immunological response, mucosal adhesion by eliciting biological responses, and anti-microbial action.

The aim of this study was to evaluate the effect of PRF and Axiostat (A chitosan-based product) on hemostasis after tooth extraction among cardiac patients on antiplatelet medication.

This prospective study was carried out on 300 patients undergoing tooth extraction. Participants were divided into two categories (n = 150, respectively) as Group I (PRF dressing) and Group II (Axiostat dressing). Time to achieve hemostasis was observed using a stopwatch. Average pain score calculation was performed using visual analog on the 7-day postoperative period. Descriptive statistics were done, and data analysis was performed using the Mann–Whitney U-test. P < 0.5 and < 0.001 were considered statistically significant and extremely significant, respectively.

Average pain score was 1.86 ± 0.06 in Group I and 1.05 ± 0.87 in Group II. Thus, lower postoperative pain was seen with Axiostat dressing. Hemostasis was achieved in Group II participants in 1.25 ± 0.06 min and in 1.89 ± 0.54 min in Group I. P < 0.01 was obtained, although no statistically significant difference in postoperative pain scores (P = 0.8) was seen.

Chitosan is a superior wound dressing material in achieving hemostasis in cardiac patients on antiplatelet medication after tooth extraction.

• Axiostat
• cardiac
• extraction
• hemostasis
• pain
• platelet-rich fibrin

Platelet-rich blood concentrates have been used to accelerate healing process in wounds and in bones since many decades worldwide. Platelet-rich fibrin (PRF) is a relatively new and established therapy, utilizing platelets and leucocytes trapped in fibrin matrix, for the treatment of non-healing ulcers and wounds. Many large series are available in this subject to prove its efficacy. Our patient, a known case of eczema, had applied slaked lime (calcium hydroxide) over an eczematous lesion on right leg and surrounding area, after which he developed deep wound with extensive erythema and blisters initially, which healed with necrosis due to patient’s neglect, in about 2 weeks. On presentation to us, the lesion had undergone necrosis and hence decision to debride the lesion was taken. After debridement, a large defect was created, which we tried treating conservatively using PRF therapy primarily, followed by pressure dressing. High dose vitamin C was given orally. The patient required antibiotics intermittently. The patient responded well to this protocol and the wound defect was closed within a few weeks.

• angiography
• biomaterials
• embolization
• platelet rich fibrin
• point-of-care

• Animals
• Embolization, Therapeutic/methods
• Embolization, Therapeutic/instrumentation
• Biocompatible Materials/chemistry
• Swine
• Catheters
• Humans
• Iliac Artery
• Renal Artery

• R01HL140951 NIH HHS
• R01 HL140951 NHLBI NIH HHS
• R01EB024403 NIH HHS
• R01 EB024403 NIBIB NIH HHS
• R01HL137193 NIH HHS
• Mayo Clinic
• R01 HL137193 NHLBI NIH HHS

• Autografts
• Systematic review; Palatal gingival harvesting
• Wound healing

• Blood platelets
• Fibrin
• Platelet-rich fibrin
• Wound healing

• LSCC
• PRF
• animal model
• reduction
• regeneration

• alveolar bone grafting
• bone remodeling
• platelet-rich fibrin
• platelet-rich plasma
• tooth extraction
• tooth socket

• Integra
• Pain
• Platelet-rich fibrin
• Take rate
• Texture change

• calcium sulfate
• extraction socket
• platelet-rich fibrin
• platelet-rich protein
• soft tissue healing

• HMGB1
• NF-κB
• palatal mucosa
• wound healing

• Animals
• Antibodies, Monoclonal/chemistry
• Gene Expression Regulation
• Gingiva/injuries
• Gingiva/metabolism
• HMGB1 Protein/genetics
• HMGB1 Protein/metabolism
• Immunohistochemistry
• Keratinocytes/metabolism
• Keratinocytes/pathology
• Maxilla/injuries
• Maxilla/metabolism
• Mice
• Mice, Knockout
• Mouth Mucosa/injuries
• Mouth Mucosa/metabolism
• NF-kappa B p50 Subunit/genetics
• NF-kappa B p50 Subunit/metabolism
• Palate, Hard/injuries
• Palate, Hard/metabolism
• Proliferating Cell Nuclear Antigen/genetics
• Proliferating Cell Nuclear Antigen/metabolism
• RNA, Small Interfering/genetics
• RNA, Small Interfering/metabolism
• Receptor for Advanced Glycation End Products/antagonists & inhibitors
• Receptor for Advanced Glycation End Products/genetics
• Receptor for Advanced Glycation End Products/metabolism
• Signal Transduction
• Tissue Culture Techniques
• Vascular Endothelial Growth Factor A/genetics
• Vascular Endothelial Growth Factor A/metabolism
• Wound Healing/genetics

The aim of this article is to review the outcomes of platelet preparations in dentistry. A structured electronic search discovered 348 articles, which described the use of autologous platelet concentrates with a relevance to clinical dentistry. Among these articles, 220 articles investigated platelet rich plasma, 99 investigated platelet rich fibrin, 22 investigated plasma rich in growth factors and 7 investigated the use of concentrated growth factors. Several studies reported beneficial treament outcomes in terms of enhanced bone and soft tissue regeneration.

• Platelet concentrates
• Platelet rich plasma
• Platelet rich fibrin
• Concentrated growth factors
• Growth factors
• Dentistry
• Plasma rich in growth factors

• Gingival enlargement
• metronidazole
• phenobarbitone
• phenytoin
• platelet rich fibrin