Phytoestrogens, with estrogenic activity, are commonly found across the Fabaceae family. Here we develop methods that use phylogeny and ethnomedicinal information in order to identify candidate species for novel phytoestrogens.

We selected Fabaceae species traditionally used as aphrodisiacs or with applications to control fertility (aphrodisiac-fertility species), to create a cross-cultural dataset of ethnomedicinal use. Using a phylogeny of the Fabaceae, "hot nodes" methods were used to identify lineages with a higher number of species with aphrodisiac-fertility uses. The known distribution of estrogenic flavonoids was used to determine whether the phytoestrogen-containing species was associated with aphrodisiac-fertility "hot nodes". Additionally, we examined the overlap of aphrodisiac-fertility uses with neurological applications, hypothesising that such species may have bioactive compounds with estrogenic properties. Lastly, the "aphrodisiac-fertility hot node" lineages without previously known estrogenic flavonoids were identified.

We showed species in aphrodisiac-fertility hot nodes were more likely to contain estrogenic flavonoids (21% of species), a major group of phytoestrogens, compared to Fabaceae in the phylogeny (11% of species). Additionally, when aphrodisiac fertility species are limited to those with neurological applications, 62% of the species within hot nodes contain estrogenic flavonoids.

We recognised 43 high-priority hot nodes, these lineages might represent promising targets for future studies on phytoestrogens. The results demonstrated the combining phylogenetic and ethnomedicinal data to guide the discovery of estrogenic flavonoids with therapeutic potential.

Cancer is a deadly disease and is one of the primary causes of mortality worldwide. Cancer therapy presents significant challenges, such as chemotherapy resistance, high toxicity, recurrence, and metastasis. As a result, the development of novel therapeutic agents for cancer continues to be a top goal to expand the number of efficient treatments available. The advent of nanotechnology is an important turning point in several scientific disciplines. Owing to the increasing difficulty of this problem, researchers have begun to focus their attention on the possibility of employing plants or extracts from plants as a potential tumor treatment. More than 3,000 medicinal plant species have been documented worldwide for their utilization in cancer treatment. Nevertheless, crude plant extracts lack specificity, and their dosages are not clearly specified. To enhance the therapeutic efficacy of these natural substances, researchers have used them in conjunction with silver nanoparticles (AgNPs). Plants possess intricate phytochemical components including sugars, polyphenols, amino acids, flavonoids, terpenoids, alkaloids, and proteins, which can function as reducing and stabilizing agents. In recent years, the application of plant-derived AgNPs has increased significantly, particularly in cancer treatment. These green-synthesized AgNPs are regarded as outstanding tools for the detection of cancer and targeted drug delivery at the tumor site. By leveraging the distinctive characteristics of nanoparticles and the antioxidant and anticancer qualities of plants, these green-synthesized AgNPs selectively eradicate tumor cells while sparing normal healthy cells. This comprehensive review aimed to summarize the key aspects of plant extracts as anticancer agents, biosynthesis of AgNPs, and recent advancements in the antitumor efficacy of green-synthesized AgNPs.

Hypericum hircinum L., commonly known as goat St. John's wort or stinking tutsan, is a medicinal plant native to the Mediterranean basin and widespread across Europe and parts of the Middle East. It has a long history of traditional uses in folk medicine to treat respiratory diseases, wounds, and burns and to relieve migraine, rheumatism, and muscular pains. Despite numerous scientific studies shading light on the phytochemical profile and on the beneficial properties of the plant extracts, a comprehensive overview of the current knowledge is missing. In this paper, we summarized the available information on botany, traditional uses, phytochemistry, and pharmacological properties of Hypericum hircinum from peer-reviewed articles published till March 2025 in PubMed, ScienceDirect, Wiley, Springer, ACS, Scielo, and Web of Science databases. The presence of numerous valuable compounds, including terpenes, phenolic acids, flavonoids, and phloroglucinols, is reported as well as the wide range of pharmacological properties, such as antimicrobial, antifungal, antiviral, antidepressant, anti-collagenase, anti-α-glucosidase, and antioxidant activities, together with non-pharmacological properties. The data reported in this review contribute to a deeper understanding of the biological properties of the species and pave the way for further investigation of its potential applications.

Essential oils derived from aromatic and non-aromatic plant sources, are recognized for their in vivo potential therapeutic benefits, including anti-oxidant, anti-inflammatory, anti-cancer, immunomodulatory and other regulatory roles by the scientific community. However, translation of such in vivo therapeutic application in clinical field is not yet available. This study examines the pre-clinical scientific studies on varied administration methods of essential oils for therapeutic purposes. The focus of this study is to explore the different administration routes, which are crucial for optimizing the delivery of these bioactive compounds for internal administration and improving their therapeutic effectiveness. Traditional and innovative methods, such as inhalation, dermal application, oral ingestion, intraperitoneal injection, and intravenous infusion are investigated. Each route has its advantages and challenges, which may impact the bioavailability, systemic distribution and toxicity of essential oils. However, such comparative pharmacokinetic and toxicity studies are lacking for the essential oils. The available basic information regarding in vivo bioavailability and toxic reactions of essential oils are discussed briefly in this review. The research findings have significant implications for developing targeted and personalized essential oil treatments, advancing integrative medicine and complementary therapy approaches. The analysis of various in vivo therapeutic study findings implies that a promising therapeutic efficiency can be achieved with essential oils at different dose ranges for different routes of administrations. The comparative analysis on therapeutic effects of different routes of administration and methods essential oil extraction are lacking. The bioavailability and toxicity studies are also limited for these therapeutic studies. Furthermore, the detailed molecular mechanism of in vivo therapeutic actions is not yet established in the studies. Ultimately, this investigation adds to the expanding knowledge base on essential oil administration routes, providing valuable guidance for healthcare professionals and researchers in the field of natural medicine. However, extensive in vivo pre-clinical studies are warranted for translation of essential oil therapy to clinical usage. Also all the traditional uses of essential oil for therapy must be validated via scientific studies.

Multi-drug-resistant (MDR) pathogens pose a significant global health challenge, underscoring the urgent need for novel antimicrobial agents with minimal toxicity to humans. This study investigated the in vitro and in silico antimicrobial and antibiofilm potentials of four essential oils (EOs): clove bud oil (CBO; Syzygium aromaticum L.), black seed oil (BSO; Nigella sativa L.), cinnamon bark oil (CNBO; Cinnamomum zeylanicum), and citronella oil (CTLO; Cymbopogon nardus L.), against 19 selected human pathogens, including MDR strains. Among the tested EOs, CBO, BSO, and CNBO exhibited the highest antibacterial activity against Staphylococcus epidermidis, with the mean zone of inhibition diameters (ZIDs) of 20.0 ±  0.2 mm, 46.0 ±  0.3 mm, and 32.0 ±  0.1 mm, respectively, at a concentration of 10 µL/disc, while CTLO displayed no antibacterial activity. CNBO demonstrated superior antifungal activity, with the mean ZIDs of 49.0 ±  0.3 mm and 36.0 ±  0.3 mm for Candida albicans and Aspergillus niger, respectively. Molecular docking analyses revealed robust interactions of key bioactive compounds-eugenol (EU) from CBO, thymoquinone (TQ) from BSO, cinnamaldehyde (CN) from CNBO, citronellal (CIT) and linalool (LIN) from CTLO-with microbial target proteins, substantiating their antimicrobial and antibiofilm potential. Notably, CTLO, despite limited in vitro activity, exhibited unique binding interactions in silico, suggesting potential niche applications. These findings underscore the translational potential of EOs as alternative antimicrobial therapies against MDR infections, particularly biofilm-associated infections, and highlight the need for further in vivo studies to validate their efficacy and safety.

In this study, the potential anti-tyrosinase, antioxidant, and ultraviolet (UV) absorption efficiency of the extracts of different parts of Castanea mollissima were evaluated. The leaf extract exhibited the most potential antioxidant effect with 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging abilities of >90%. C. mollissima extracts showed high UV-A and UV-B absorption efficiency. In addition, screening of the leaf extract demonstrated C. mollissima leaves as a rich source of tyrosinase inhibitors with maximum inhibitory activity and half-maximal inhibitory concentration values of 96% and 336.43 µg/mL, respectively. This is the first-ever report of screening of leaf extract for its potential activities. Eighteen phyto-compounds were identified from the C. mollissima leaf extract using gas chromatography-mass spectrometry and high-performance liquid chromatography techniques. Of these, the content of compounds 10, 11, 12, 13, 14, and 16 in the C. mollissima leaf extract was significantly high (14.35-88.42 µg/g). The molecular docking, absorption, distribution, metabolism, excretion, and toxicity, and Lipinski's rule of five analyses indicated high interaction efficacy of all the major compounds (docking score values of -6.75 to -8.89 kcal/mol) with the target enzyme tyrosinase, had drug properties, and were non-toxic for human use.

Metabolic diseases, such as diabetes, pose significant risks to human health due to their complex pathogenic mechanisms, complicating the use of combination drug therapies. Natural medicines, which contain multiple bioactive components and exhibit fewer side effects, offer promising therapeutic potential. Metabolite imbalances are often closely associated with the pathogenesis of metabolic diseases. Therefore, metabolite detection not only aids in disease diagnosis but also provides insights into how natural medicines regulate metabolism, thereby supporting the development of preventive and therapeutic strategies. Deep learning has shown remarkable efficacy and precision across multiple domains, particularly in drug discovery applications. Building on this, We developed an innovative framework combining graph autoencoders (GAEs) with non-negative matrix factorization (NMF) to investigate metabolic disease pathogenesis via metabolite-disease association analysis. First, we applied NMF to extract discriminative features from established metabolite-disease associations. These features were subsequently integrated with known relationships and processed through a GAE to identify potential disease mechanisms. Comprehensive evaluations demonstrate our method's superior performance, while case studies validate its capability to reveal pathological mechanisms in metabolic disorders including diabetes. This approach may facilitate the development of natural medicine-based interventions. Our data and code are available at: https://github.com/Lqingquan/natural-medicine-discovery.

The objective of the present study was to assess the patterns of trace element and mineral accumulation in 21 medicinal plants collected in the Altai Mountains foothills. The levels of trace elements and minerals in Hippophae rhamnoides, Aronia melanocarpa, Sorbus aucuparia, Viburnum opulus, Rosa canina, Crataegus sanguinea (fruits), Chamaenerion angustifolium, Echinacea purpurea, Fagopyrum esculentum, Trifolium pratense, Origanum vulgare, Achillea millefolium, Mentha piperita (herbs), Plantago major, Salvia officinalis, Urtica dioica (leaves), Panax ginseng, Rhaponticum carthamoides, Eleuterococcus senticosus, and Taraxacum officinale (roots), and Matricaria recutita flowers were assessed using inductively-coupled plasma mass-spectrometry. Comparative analysis revealed that R. carthamoides roots accumulated the highest level of toxic trace elements (Al, Cd, Ni, Pb). C. angustifolium herbs and V. opulus fruits are characterized by the highest As and Sn levels, respectively. The highest content of essential trace elements and minerals was observed in H. rhamnoides fruits (Zn), V. opulus fruits (Cu), E. purpurea herbs (I, Mg), R. carthamoides roots (Fe), O. vulgare herbs (Mn), and M. recutita herbs (Se). In turn, R. canina fruits are characterized by the lowest levels of certain essential and toxic trace elements. Significant differences in B, Cr, Li, Si, and Sr content were also revealed. Discriminant analysis revealed complete discrimination of certain medicinal plants based on their trace element and mineral content. The obtained data indicate the potential usefulness of medicinal plants as sources of certain essential trace elements and minerals, as well as provides data for risk assessment of toxic trace element intake following administration of medicinal plants.

Traditionally, Pteridium aquilinum L. has been utilized as medicine for ages, however, it is not listed in the Nigerian herbal pharmacopeia, and there is no information regarding its standardization and antimicrobial activity. Therefore, the purpose of this study was to examine the pharmacognostic parameters and antimicrobial activity of Pteridium aquilinum leaf.

Macroscopy, chemo-microscopy, fluorescence, and microscopic analyses of the leaf were investigated using standard methods. Qualitative and quantitative phytochemical screening, thin layer chromatography (TLC), GC-MS, and FTIR were also determined using standard procedures. Antioxidants were evaluated using DPPH. The antimicrobial activities of methanol extract and fractions were evaluated using Agar well diffusion method against Candida albicans, Aspergillus niger, Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, and Pseudomonas aeruginosa. The macroscopic features of P. aquilinum leaf include a bi-pinnate leaflet and alternate pinna arrangement. The GC-MS-identified compounds in the most active (DCM fraction) were docked against Candida albicans Sterol 14-alpha demethylase (5TZ1) and Escherichia coli DNA gyrase subunit B (6YD9).

The macroscopic features and microscopic features such as anomocytic stomata, numerous stomata in the abaxial layer, and absence of stomata in the adaxial layer were observed. Chemomicroscopy of the powdered leaves shows that the leaf contains tannins, starch, and lignin. GC-MS detected eighteen compounds. The antimicrobial test revealed that the dichloromethane fraction of P. aquilinum leaf was most active on all the test strains (bacteria and fungi) at 25 mg/mL to 100 mg/mL concentrations. Through in silico research, the binding of 1,2-benzenedicarboxylic acid, (4-hydroxybenzoyl) hydrazine, octadecadienoyl chloride, and 11,14-Eicosadienoic acid, detected in the DCM fraction by GC-MS analysis, to the active sites of 5TZ1 and 6YD9 was stable.

This research gave scientific credence to the traditional medical practice of treating infections with P. aquilinum leaves.

This study investigates the effect of thermal treatment on the phytochemical composition and bioactivities of Rumex vesicarius, focusing on its antioxidant, antidiabetic, and anti-biofilm properties. Quantitative analysis showed that at ambient temperature, the extract had the highest phenolics (57.89 mg GAE/g), flavonoids (19.45 mg QE/g), tannins (12.78 mg CE/g), flavonols (6.48 mg), and anthraquinones (2.078 mg). At 60°C, it retained significant phenolics (52.89 mg GAE/g) and flavonoids (18.45 mg QE/g) with minimal degradation. At 90°C, phenolics decreased slightly (43.59 mg GAE/g), but enzymatic inhibition and antimicrobial properties improved. β-Carotene stability varied, with untreated extract at a minimum inhibitory concentration (IC50) of 118.136 µg/mL. Peak antioxidant activity was at 150°C (IC50 = 102.77 µg/mL), with degradation above 150°C. Antidiabetic potential, via α-amylase and α-glucosidase inhibition, showed the lowest IC50 values (92.106 µg/mL for both) at 90°C. Beyond 120°C, IC50 rose to 268.35 µg/mL (α-amylase) and 268.31 µg/mL (α-glucosidase) at 210°C. Anti-biofilm activity peaked at 90°C (IC50 = 33.55 µg/mL), with untreated and 60°C extracts showing strong inhibition (∼80%-90%). Above 90°C, inhibition dropped, reaching IC50 of 253.53 µg/mL at 210°C. Moderate heating (60-90°C) optimizes bioactive availability, positioning R. vesicarius for pharmaceutical and nutraceutical applications.

Two new stigmastane steroids (1 and 2) were isolated from the methanol extract of the leaves of Vernonia glabra, together with seventeen known compounds (3-19) including one fatty acid, four triterpenoids, four steroids, one trinitropropanoyl glucoside, and seven flavonoids. The structures of compounds 1 and 2 were assigned based on their IR, NMR and MS data, and by comparison with literature values. The MeOH extract, its fractions and isolated compounds were subjected to in vitro antibacterial assay against two Gram-positive (Staphylococcus aureus ATCC25923 and Streptococcus pneumoniae ATCC49619) and two Gram-negative (Escherichia coli ATCC8739 and Klebsiella pneumoniae ATCC10031) bacteria, using broth microdilution method. The extract and fractions exhibited (16 ≤ MIC ≤ 512 μg/mL) antibacterial activities. The isolated and tested compounds were also active (16 ≤ MIC ≤ 128 μg/mL) against the four pathogenic bacteria, with compound 2 being the most active and E. coli, the most sensitive microorganism.

Sarcochlamys pulcherrima (Roxb.) Gaudich is a small, traditionally used medicinal plant belonging to the Urticaceae family. The leaves of the plant are commonly consumed by different tribes inhabiting the northeastern states of India. This work involves dereplicating ethanolic leaf extract of S. pulcherrima, leading to the isolation of two new steroidal compounds-SP-C1 and SP-C2-characterised by single crystal x-ray diffraction analysis. The compound SP-C2 displayed significant antioxidant properties with an IC50 value of 4.75 ± 0.22 µg mL-1 in the 2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging assay along with ferric reducing antioxidant power (FRAP) value of 1792.40 ± 0.11 µM, which is comparable to that of the employed standard antioxidant ascorbic acid (2032.60 ± 0.12 µM). SP-C2 also exhibited significant α-glucosidase inhibitory potential with an IC50 value of 506.53 ± 0.24 µg mL-1 in close resemblance with that of the standard α-glucosidase inhibitor acarbose (420.32 ± 0.62 µg mL-1).

Human colon cancer and prostate cancer are the most common malignancy globally. Despite the availability of many treatments, resistance to traditional medicines, such as chemotherapy and radiotherapy, remains a severe obstacle in cancer treatment. Hence, searching for new therapeutic options is of utmost priority. The present investigation evaluates the in-silico and in-vitro anticancer potential of phytochemicals of Piper chaba. The cytotoxicity results demonstrated that the plant extract exhibited significant anticancer activity, with IC50 values of 12.66 ± 0.25 µg/mL for HCT-116 and 19.49 ± 0.37 µg/mL for DU-145. Molecular docking and molecular dynamics simulations were performed to explore the interaction of potential drug targets with the phytochemicals. Subsequently, pharmacokinetic parameters calculations were performed to evaluate the drug-likeness. Piperine exhibits the highest binding affinity for vascular endothelial growth factor receptor-2 (VEGFR2) protein with a docking score of -9.71 kcal/mol. Sylvatine had a greater binding affinity for human epidermal growth factor receptor 3 (HER3) protein than the other phytochemicals. Isopiperine, chabamide, Piperlonguminine, Santamarine and Versalide only exhibited ligand activity for human IKK beta protein (inhibitor of kappa B kinase). Additionally, a principal component analysis was performed to strengthen the investigation's scope. These proposed phytochemicals are reported to possess potential VEGFR2, HER2 and human IKK beta inhibition. The best phytochemical hits have excellent binding affinity and hold a massive anticancer potential, opening up new avenues for prospective future investigations in cancer treatment.

Ethnobotany investigates the ways in which communities utilize plant species to tackle a range of health concerns in both humans and animals, highlighting the intricate relationships between plant life and local cultural practices. The degradation of habitats resulting from agricultural development and deforestation poses a considerable risk to the accessibility of these vital plants. This research was conducted in the Fofa and Toba sub-districts of the Yem Zone in Central Ethiopia, aiming primarily to explore and record the medicinal plant species that indigenous communities use for treating various health issues in both people and livestock.

This study was carried out between March 2023 and April 2024, involving a total of 96 informants, with 12 selected from each kebele. A range of quantitative methodologies were employed in the research, such as the informant consensus factor (ICF), fidelity level (FL), plant part value, preference ranking, and direct matrix ranking. Furthermore, various statistical analyses including independent t-tests, one-way ANOVA, correlation, and regression were performed using R to evaluate and compare the ethnobotanical knowledge among different groups of informants.

A total of 164 medicinal plant species from 140 genera and 60 families were identified in the study. Of these species, 67.68% were utilized for human ailments, 19.5% for livestock issues, and 12.8% for both. The sources of these medicinal plants included 81 species from wild areas, 35 from home gardens, 23 from agricultural fields, 15 from living fences, and 10 from roadsides. In terms of growth forms, herbs comprised the largest group with 76 species, followed by trees with 42 species, shrubs with 34 species, and climbers with 9 species. The most commonly used plant parts were leaves, followed by roots. Preparations were primarily made by crushing the plants, with other methods including powdering, chewing, smashing, and boiling. The highest informant consensus factor value of 91% indicated a significant healing potential for respiratory diseases, common colds, coughs, and fevers. Notably, A. sativum (for malaria) and H. rueppelii (for abdominal pain) recorded the highest fidelity level values. Additionally, the average number of medicinal plants reported by participants varied significantly across different demographic factors, including gender, age groups, educational levels, and experience (P < 0.05).

The study area is home to a wide variety of potential medicinal plants along with valuable indigenous knowledge. To address the growing anthropogenic threats and safeguard these plants and their associated knowledge, it is essential to adopt effective conservation strategies and promote responsible use. Additionally, the medicinal properties of these plants should be scientifically validated to harmonize local knowledge with modern medicine effectively.

Detarium microcarpum, is a species confined to drier regions of west and central Africa used to treat various diseases including cancer. Phytochemical screening revealed the presence of secondary metabolites (alkaloids) The aim of this work is to study the effect of total aqueous extracts and alkaloid fractions from D. microcarpum leaves, bark and roots on Nicotiana tabacum L. cv. 'Bright Yellow 2' (BY-2) tobacco cell line GFP-TuA3 expressing a N-terminal fusion of GFP. The plant was harvested in two different regions of Mali with a contrasting climate. The effects of the extracts on the microtubules was followed by spinning disc confocal microscopy. We showed that the anti-microtubular effect of the extracts is dose-dependent, depends of the sampling site and the part of the plant used. Total alkaloids extracted of D. microcarpum bark have more effect on microtubules than leaf and root. The bioactivity-guided fractionation should be used to screen out the biologically active compounds of the total alkaloid extracts of the bark of D. microcarpum.

World Health Organization (WHO) has reported 249 million cases infected by malaria worldwide and 608 thousands deaths in 2022. Investigations for new antimalarial drugs from traditional medicine have proven to be more effective and less expensive. The medicinal plants that have been used for treatment of malaria, generally known as types of fevers in traditional medicine, can be suitable candidates for evaluating antimalarial effects. The aim of the present study was to evaluate the in vitro mechanism of antimalarial action of selected medicinal plants by inhibition test of heme detoxification (ITHD).

The methanol extract and fractions were prepared through maceration of the dry powdered plants. The ITHD method was carried in 96-wells plate and the percentage of heme polymerization inhibition was determined. The cytotoxicity of the effective plants was examined on MDBK cell line by MTT assay. Bioassay guided fractionation was performed exposing to size exclusion column chromatography and liquid-liquid fractionation and was assayed by the ITHD method for the sample with the least IC50 value and lowest cytotoxic effect.

The methanol fraction of Viola odorata whole plant showed the most considerable results among the tested plants with IC50 171.8μg.mL-1 beside the lowest cytotoxic effects. This fraction by the bioassay guided fractionation led to fraction SB2 and this fraction demonstrated the most effective result with lowest IC50=14.8±3μg.mL-1 in ITHD assay.

Regarding the results of the present study and the traditional use of V. odorata for overcoming fever in Iranian traditional medicine, the final fraction of the plant could be proper candidate for further phytochemical and antimalarial studies.

The wild indigenous African tree, Ximenia caffra Sond also commonly known as 'sour plum' and found in Southern Africa is traditionally used as a source of food and medicine by rural communities. Its fruit has been found to have vitamins, minerals, macronutrients, other important compounds such as phenolics and flavonoids. Other parts of the plant such as the seeds, roots and the leaves are used to treat vast different ailments such as cough, cancer, sexual transmitted disease and so on. Its polyphenols compounds that contribute to its antioxidant, anti-inflammatory, anticancer and antimicrobial activities. The aim of this review is to explore X. caffra, the indigenous fruit that has health benefit of nutraceuticals and medicinal food, therapeutic capabilities in traditional medicine. The words' Ximenia cafrra' were used. The juice of X. caffra fruits comprises several potentially beneficial phytochemicals, minerals, and initial assessment indicate a remarkable antioxidant capacity of the fruit.

Lemon grass, formally identified as Cymbopogon citratus, is a plant that belongs to the Poaceae family. The present work aimed to examine the chemical composition by GC/MS analysis and assess the biological potential of C. citratus volatile oil and n-hexane extract. The volatile oil and n-hexane extract were evaluated for antioxidant potential and tested for their enzyme inhibition against tyrosinase, butyrylcholinesterase (BChE), acetylcholinesterase (AChE), α-amylase, and α-glucosidase. The chemical analysis of the lemongrass n-hexane extract (HE) and volatile oil (VO) revealed that the main constituents in the HE are aliphatic hydrocarbons (42.98%), triterpenoids (20.14%), and aromatic hydrocarbons (17.25%). Conversely, the main constituents of the (VO) are predominantly monoterpenes, namely α-citral (36.08%), β-citral (34.22%), and β-myrcene (13.84%). The oil showed more potent antioxidant potential in DPPH, ABTS, CUPRAC, FRAP, and phosphomolybdenum (10.18, 35.69 mg Trolox equivalent/g, 98.97 and 69.73 mg Trolox equivalent/g and 43.01 mmol Trolox equivalent/g). The HE displayed higher BChE (1.53 mg Galanthamine equivalent)/g), as well as α-amylase and α-glucosidase inhibitory activities (0.39 and 2.40 mmol Acarbose equivalent/g). The VO demonstrated more potent tyrosinase inhibitory activities (57.19 mg Kojic acid equivalent/g) along with acetyl and butyrylcholinesterase inhibition. Dominant compounds exhibited the ability to bind with high affinity to various target proteins, with a particular affinity for AChE and BChE. The volatile oil and n-hexane extract of C. citratus show significant promise as a viable choice for the advancement of novel therapeutic strategies aimed at addressing oxidative stress, neurodegeneration, and diabetes.

Botanical and herbal supplements contain diverse compounds that support health and prevent disease by influencing biological pathways. In ruminant nutrition, such supplements are rarely used as most integrations focus on production improvements using synthetic additives. This review explores the potential of Mediterranean herbs, namely, garlic, oregano, and rosemary, in dairy ruminant diets, emphasizing their effects on milk production, antioxidant activity, weaning management, in vitro studies, and economic impacts. Using medicinal plants reduces reliance on synthetic additives, lowers costs, enhances animal health, and boosts productivity. These plants align with sustainable farming practices, enable premium market access, and support local resources to improve performance and reduce livestock's environmental footprint.

Ceratonia siliqua L., is a species of significant nutritional and industrial interest with extensive traditional uses. This fabaceae is renowned for its medicinal properties, including the treatment of high blood pressure. Due to its chemical composition, carob exhibits several valuable therapeutic functions such as antioxidant, antidiarrheal, antidiabetic, and antibacterial actions.

This study investigates the chemical composition of Ceratonia siliqua L. leaves aqueous extract (CsAE) and explores the vasorelaxant effect and its underlying mechanisms. Acute toxicity and antioxidant activity of CsAE were also examined.

The phytochemical profile was elucidated using TLC and UHPLC-MS. The vasorelaxant effect and mechanisms were studied on thoracic aortic rings from normotensive rats, using various antagonists. Acute toxicity was assessed by orally administering the extract to mice. Antioxidant activity was evaluated using β-carotene bleaching and DPPH.

TLC analysis of CsAE reveals flavonoids and hydrolysable tannins. Gallic acid, myricitrin, quercitrin as well as galloylglucopyranoside derivatives were identified by UHPLC-MS. CsAE relaxed phenylephrine-precontracted aorta in a concentration-dependent manner. This response was reduced when the aorta was denuded or pretreated with L-NAME, hydroxocobalamin, ODQ, 4-AP, TEA, calmidazolium chloride, and thapsigargin. CsAE showed significant antioxidant activity with no observed toxicity in the experimental animals.

CsAE has a significant vasodilatory effect, mediated through the CaM/eNOS/sGC pathway, activation of Kca and Kv, and intracellular calcium mobilization into SERCA. It also exhibits strong antioxidant activity, with no observed toxicity in the experimental animals. These findings represent the first evidence of the vasorelaxant effect of Ceratonia siliqua L. leaves from Eastern Morocco.

This paper presents a comprehensive overview of ethnobotanical knowledge in Calabria, southern Italy. The diverse plant uses and knowledge in the region stem from the cultural contributions of various populations that have inhabited the area since ancient times. To achieve the stated objective, an in-depth review of 16 bibliographic sources published between 1950 and 2024 was conducted, and data on ethnobotanical uses was extracted. The data were then analyzed using various indices, including CI, CV, FC, PPV, RFC, RI, and UV, to determine the most relevant species, families, and plant parts. A total of 4873 records were collected. The analysis shows that the Asteraceae family is the most used, while Urtica dioica L. and Sambucus nigra L. are the most frequently used species according to the CV index. Medicinal and alimentary purposes are the most common types of use. This study could serve as a foundation for further detailed research, contributing to the valorisation of the ethnobotanical heritage of this region.

A method to detect camphor gas is considered indispensable in the pharmaceutical industry. Unfortunately, the available sensors to detect the presence of camphor in the air are very limited and still on a laboratory scale, such as using chromatography-mass spectroscopy (GC-MS). The research's main focus is to obtain a portable sensing system with excellent sensitivity and selectivity. This study explored polyaniline (PANi) concentrations cast over PVAc nanofiber as a matrix to detect camphor gas using a quartz crystal microbalance (QCM) system to measure camphor exposure. Scanning electron microscopy (SEM) and Fourier-transform infrared (FTIR) were used to analyze the morphology and chemical composition of the fabricated active layer (i.e., nanofiber with PANi thin film). Increasing the PANi concentration provides more PANi on the sensor surface, thus amassing the active groups to interact with camphor gas molecules. It shows that a sensor with a 0.08% PANi thin film (Nano-PANi8) has a sensitivity of 2.594 Hz ppm-1, much greater than the sensor without PANi, which is only 0.305 Hz ppm-1. In addition, the sensor also has good repeatability and rapid response and recovery time of 47 s and 133 s, respectively. Compared to other gaseous compounds, the sensor also has excellent selectivity for camphor and robust long-term stability over three weeks of testing. The produced QCM sensor employing PANi thin film can give a camphor sensor superior performance, including excellent sensitivity, selectivity, and long-term stability. Furthermore, the use of QCM as a base sensor also makes the fabricated sensor portable.

Cancer is one of the most complicated and prevalent diseases in the world, and its incidence is growing worldwide. Natural products containing pharmacological activity are widely used in the pharmaceutical industry, especially in anticancer drugs, due to their diverse structures and distinctive functional groups that inspire new drug results by means of synthetic chemistry. Terrestrial medicinal plants have traditionally been the primary source for developing natural products (NPs). However, over the past thirty years, marine organisms such as invertebrates, plants, algae, and bacteria have revealed many new pharmaceutical compounds known as marine NPs. This field constantly evolves as a discipline in molecular targeted drug discovery, incorporating advanced screening tools that have revolutionised and become integral to modern antitumor research. This review discusses recent studies on new natural anticancer alkaloids obtained from marine organisms. The paper illustrates the structure and origin of marine alkaloids and demonstrates the cytotoxic action of new alkaloids from several structural families and their synthetic analogs. The most recent findings about the potential or development of some of them as novel medications, together with the status of our understanding of their current mechanisms of action, are also compiled.

Medicinal plants have long played a crucial role in healthcare systems, but limited genomic information on these species has impeded the integration of modern biological technologies into medicinal plant research. In this study, we selected nine common medicinal plants, each belonging to a different plant family, including Sarcandra glabra (Chloranthaceae), Nekemias grossedentata (Vitaceae), Uraria crinita (Fabaceae), Gynostemma pentaphyllum (Cucurbitaceae), Reynoutria japonica (Polygonaceae), Pseudostellaria heterophylla (Caryophyllaceae), Morinda officinalis (Rubiaceae), Vitex rotundifolia (Lamiaceae), and Gynura formosana (Asteraceae), to estimate their genome sizes and conduct preliminary genomic surveys. The estimated genome sizes by flow cytometry were 3.66 Gb, 0.65 Gb, 0.58 Gb, 1.02 Gb, 3.96 Gb, 2.99 Gb, 0.43 Gb, 0.78 Gb, and 7.27 Gb, respectively. The genome sizes of M. officinalis, R. japonica, and G. pentaphyllum have been previously reported. Comparative analyses suggest that variations in genome size may arise due to differences in measurement methods and sample sources. Therefore, employing multiple approaches to assess genome size is necessary to provide more reliable information for further genomic research. Based on the genome survey, species with considerable genome size variation or polyploidy, such as G. pentaphyllum, should undergo a ploidy analysis in conjunction with population genomics studies to elucidate the development of the diversified genome size. Additionally, a genome survey of U. crinita, a medicinal plant with a relatively small genome size (509.08 Mb) and of considerable interest in southern China, revealed a low heterozygosity rate (0.382%) and moderate repeat content (51.24%). Given the limited research costs, this species represents a suitable candidate for further genomic studies on Leguminous medicinal plants characteristic of southern China. This foundational genomic information will serve as a critical reference for the sustainable development and utilization of these medicinal plants.

Natural compounds from plants represent suitable options to replace synthetic biocides when employed against microorganisms in various applications. Essential oils (EOs) have attracted increased interest due to their biocompatible and rather innocuous nature, and complex biological activity (fungicide, biocide and anti-inflammatory, antioxidant, immunomodulatory action, etc.). EOs are complex mixtures of derived metabolites with high volatility obtained from various vegetal parts and employed to a great extent in different healthcare (natural cures, nutrition, phyto- and aromatherapy, spices) and cosmetics applications (perfumery, personal and beauty care), as well as in cleaning products, agriculture and pest control, food conservation and active packaging, or even for restauration and preservation of cultural artifacts. EOs can act in synergy with other compounds, organic and synthetic as well, when employed in different complex formulations. This review will illustrate the employment of EOs in different applications based on some of the most recent reports in a systematic and comprehensive, though not exhaustive, manner. Some critical assessments will also be included, as well as some perspectives in this regard.

Natural product therapy has been used to treat illness for thousands of years, and modern-day medicines, such as various anticancer, antihypertensive, and antimigraine drugs, have been developed from natural products. Natural medicines are advantageous as they tend to have fewer side effects and are considered a relatively safe option. Solanum aethiopicum L. (S. aethiopicum) is a vegetable crop of the Solanaceae family and is considered one of the five most important crops in sub-Saharan Africa, alongside tomatoes, onions, peppers, and okra. S. aethiopicum has many health benefits as it contains the three major macronutrients (carbohydrates, proteins, and fats) as well as fiber and many essential vitamins. Additionally, much research has been conducted on the medicinal value of S. aethiopicum over the past few decades. S. aethiopicum has been found to have many pharmacological properties including anti-inflammatory, antibacterial, antidiabetic, anti-obesity, and antioxidant effects. Currently, to our knowledge, there are no comprehensive reviews of the numerous studies on S. aethiopicum. Therefore, in this review, we summarize the nutritional, phytochemical, and pharmacological analyses of S. aethiopicum, identify notable effects, and review the results.

The application of plant extracts and their phytochemicals as potential treatments for bacterial illnesses has increased significantly in the last few decades. In Ethiopia, Echinops kebericho Mesfin is widely used to treat a range of illnesses in humans and animals. This study aimed to evaluate the antibacterial activity and phytochemical screening of Echinops kebericho Mesfin.

We carried out an in vitro experimental study after collecting the plants from their natural habitats. Then macerated in absolute methanol and petroleum ether solvents and concentrated the extracts using a rotary evaporator. In the experiment, we used Standard cultures of E. coli, K. pneumoniae, S. aureus, and P. aeruginosa. The agar-well diffusion method evaluated the antibacterial activity of the plants. The agar dilution method determined the minimum inhibitory concentration of the plant extract.

The percentage yield of the plant extracts ranged from 6.25% to 7.85%. The methanol extract of Echinops kebericho Mesfin had the highest inhibitory effect on S. aureus (ATCC 25923) (16.67 ± 0.58 mm), followed by E. coli (ATCC 25922) (11.0 ± 1.73 mm). Phytochemical screening of leaves from the methanol and petroleum ether extracts of the plant revealed the presence of phytochemicals such as alkaloids, flavonoids, tannins, and cardiac glycosides. The present study revealed that the extracts of these plants have antibacterial activity. However, researchers should conduct further studies on the safety margin and quantitative bioactive isolation of selected medicinal plants.

Boosting the immune system has become a crucial aspect in the global battle against the COVID-19 pandemic and other similar infections to protect oneself against symptoms, especially in the prevention of viral infections of the lower respiratory tract. The importance of conducting more studies to create successful herbal formulations as infection prevention measures is emphasized in this review, which looks at the function of immune-boosting nutrients, medicinal plants, and herbal treatments. We reviewed and analyzed 207 studies published from 1946 to the present using reputable databases like Google Scholar, PubMed, and NCBI. The review examined 115 plant species in total and identified 12 key nutrients, including vitamins A, D, C, omega-3 fatty acids, iron, and zinc, while noting that four plant families, Rosaceae, Asteraceae, Amaryllidaceae, and Acanthaceae, show potential against respiratory infections like influenza, RSV, and SARS-CoV. To lower the risk of infection, it is recommended to consume nutritious meals that have immune-modulating qualities. Information on the bioactive components of medicinal herbs, spices, and plants that have been effective in treating respiratory viral infections and related conditions is compiled in this review, which highlights phytoactive substances with antibacterial and antiviral activity as effective modulators to lower the risk of infections. Furthermore, it is highlighted that ancient knowledge systems, like Ayurveda and Naturopathy, should be integrated to help develop new herbal formulations. To improve immunity and lessen vulnerability to serious respiratory infections, the results highlight the need for including immune-modulating foods and plant-based medicines into everyday routines.

In the pursuit of safer and more effective treatments, there is a growing interest in plant-derived compounds, particularly lectins, because of their diverse pharmacological properties. This study focused on isolating, purifying, and characterizing lectin from Combretum glutinosum seeds (CGSLs) to assess its potential as an analgesic and antiulcer agent. CGSL extraction involved defatting and buffer extraction, followed by purification using ammonium sulfate fractionation and fetuin-agarose affinity column chromatography. The isolectins (iso-CGSLs), each consisting of 60 kDa and 57 kDa heterodimeric subunits, displayed glycoprotein properties with a 40 % neutral sugar content. They exhibited peak activity at 55 °C and remained stable for up to the fifth day at room temperature. The activity exhibited a pH dependence, peaking between 7.5 and 10.5, and all seemingly operated independently of metal ions. CGSL, at optimal doses ranging from 6 to 12 mg/kg, had significant analgesic effects on acetic acid-induced writhing and hot plate tests in mice. Evaluation using 0.7 % acetic acid resulted in notable pain reduction across all doses (P < 0.05). The analgesic effect of lectin was partially reversed by naloxone (a morphine antagonist), indicating partial involvement of the opioid receptor system. Furthermore, CGSL exhibited antiulcer effects in ethanol-induced gastric ulcer models in rats, highlighting its therapeutic potential as a natural alternative for analgesic and antiulcer treatments.

To summarise a detailed up-to-date review of the traditional uses, phytoconstituents, and pharmacological activities of various parts of Kigelia africana.

Google Scholar, PubMed, PubChem, Elsevier, King Draw, indianbiodiversity.org.

The phytochemical analysis of Kigelia africana subsp. africana has revealed the presence of approximately 145 compounds extracted from different parts of the plant. These bioactive extracts of the plant possess anti-inflammatory, antioxidant, antimicrobial, antidiabetic, antineoplastic, and anti-urolithic activities. Due to its anti-inflammatory, antioxidant, and immune-booster properties, Kigelia can prove to be an essential source of drugs for treating various disorders.

Knowledge of the phytoconstituents, non-medicinal and medicinal traditional uses, pharmacological activities, and products obtained from Kigelia is described in this review with the hope that the updated findings will promote research on its biological pathways.

Medicinal plants and microorganisms are closely linked, with microorganisms boosting plant growth, offering pest control, and enhancing secondary compound production. However, there's a lack of systematic research, detailed molecular studies, and standardized methods for effectively using microorganisms in developing products from medicinal plants. To enhance understanding of the present research progress, emerging patterns, and key areas pertaining to microorganisms found in medicinal plants, CiteSpace bibliometric software was employed to visualize and analyze 1269 English publications sourced from the Science Net Core Collection database. Through the utilization of keyword co-occurrence analysis and cluster analysis methods, this study seeks to explore collaborative networks among countries, institutions, and scholars involved in the study of microorganisms in medicinal plants. This review highlights key research areas in microbiology, focusing on evaluating natural compounds for antibacterial properties and the impact of secondary metabolites on microbial communities, aiming to highlight significant research domains and primary focuses for researchers and professionals engaged in the field of microbiology concerning medicinal plants.

Plants have been used for a long time in traditional medicine to treat many diseases. The genus Prangos belongs to the Apiaceae family and has various medicinal and aromatic species. Since ancient times, Prangos species have been employed extensively in traditional medicine for different purposes and are especially popular for their aphrodisiac effects.

The goal of this paper is to represent a systematic review of the species in the genus Prangos, including their botanical characteristics, uses in traditional medicine, phytochemical constituents, the composition of the essential oils produced, and the biological properties.

The articles regarding traditional uses and bioactivities of Prangos species were evaluated using electronic databases such as PubMed, Google Scholar, and ScienceDirect. Use of the World Flora Online (WFO) - The Plant List, The International Plant Names Index, the World Checklist of Vascular Plants (2024), and ChemDraw Professional helped complete this compilation.

Phytochemical investigations have indicated that coumarins are characteristic constituents of Prangos species, especially prenylated simple coumarins and furanocoumarins, and also flavonoids, terpenoids, and phytosterols occur in this genus. In addition, the essential oils of these plants have been examined. The biological properties of the Prangos species seem worthy of further investigation. Also, some information about the toxicity of these species and their use as ingredients in food products is presented.

This review highlights the evaluation of traditional knowledge, phytochemical profiles, biological activities, and potential uses of Prangos species as foods and spices. Many pharmacological activities have been performed related to their traditional uses, but frequently, the exact mechanism of action remains scientifically unproven. This review has compiled data on the phytochemistry, the active secondary metabolites, the biological properties, and recent advances in Prangos species.

Considerable progress has been made in enhancing resolution in reversed-phase liquid chromatography for the analysis of complex samples, particularly within the field of natural products, through the application of global retention models using multi-linear gradients. Global models effectively differentiate solute retention effects from those originating from the column and solvent, offering predictive capabilities comparable to conventional individual retention models, without the requirement for standards for all compounds. While conventional individual models result in higher accuracy, they frequently demand standards that are unavailable for natural product samples. Moreover, the creation of individual models can be time-consuming due to the need for repetitive work for additional compounds. Experimental validation of global models has demonstrated that the accuracy is enough for the prediction of complex chromatograms. Through a carefully designed experimental work, this study reports the correct determination of global parameters for column and solvent, with excellent consistency across various medicinal plant samples. The successful transfer of predictions and optimisation of resolution across diverse plant species (lemon balm, peppermint, and pennyroyal) is confirmed. This highlights the applicability of predictions using global models across botanical varieties.

Mimusops zeyheri Sond. (Milkwood) is an indigenous fruit tree species with considerable ecological, cultural, and nutritional significance that remains underexploited. This review synthesizes current knowledge on its distribution, taxonomy, phytochemistry, ethnomedicinal applications, ecological functions, genetic diversity, and biotechnological potential. A systematic literature search, spanning 1949 to April 2024, yielded 87 relevant publications from an initial 155. Mimusops zeyheri plays a crucial role in supporting the cultural traditions and economic activities of Indigenous Southern African Communities. Its distribution encompasses South, East, and Southern Tropical Africa, with substantial populations across South African provinces. Ethnomedicinally, various plant parts treat conditions including wounds, gastrointestinal issues, and diabetes. The leaves (34%) and roots (32%) are used, with infusion (33%) and decoction (31%) as primary preparation methods. Oral administration (70%) is the most common, primarily addressing skin conditions (18%). Despite its nutritional richness, a standardized nutrient profile is lacking. Limited genetic diversity studies underscore the need for further research. This study highlights Mimusops zeyheri's multifaceted importance and research gaps, particularly in other Southern African countries. Future investigations should focus on comprehensive phytochemical analysis, ethnomedicinal validation, ecological conservation, genetic diversity assessment, and biotechnological applications. Multidisciplinary collaborations are recommended to promote sustainable utilization while preserving traditional practices.

The Yucatan Peninsula has a privileged wealth of vascular plants with which various Mayan herbal formulations have been developed. However, studies on their antipathogenic and antivirulence properties are scarce.

Identify antivirulence properties in Mayan herbal remedies and determine their antipathogenic capacity in burn wounds infected with Pseudomonas aeruginosa.

An ethnobotanical study was conducted in Mayan communities in central and southern Quintana Roo, Mexico. Furthermore, the antipathogenic capacity of three Mayan herbal remedies was analyzed using an animal model of thermal damage and P. aeruginosa infection. Antivirulence properties were determined by inhibiting phenotypes regulated by quorum sensing (pyocyanin, biofilm, and swarming) and by the secretion of the ExoU toxin. The chemical composition of the most active herbal remedy was analyzed using molecular network analysis.

It was found that topical administration of the remedy called "herbal soap" (HS) for eleven days maintained 100% survival of the animals, reduced establishment of the bacteria in the burn and prevented its systemic dispersion. Although no curative effect was recorded on tissue damaged by HS treatment, its herbal composition strongly reduced swarming and ExoU secretion. Through analysis of Molecular Networks, it was possible to carry out a global study of its chemical components, and identify the family of oxindole monoterpenoid alkaloids and carboline and tetrahydropyrididole alkaloids. In addition, flavonols, flavan-3-ols, and quinic acid derivatives were detected.

The antipathogenic and antivirulence capacity of ancient Mayan remedies makes them a potential resource for developing new antibacterial therapies to treat burns infected by P. aeruginosa.

Plant-derived compounds have recently garnered significant interest in the field of medicine due to their rich repertoire of phytochemicals, which holds promise for exploring novel therapies to treat cancer. This study embarks on the first-time investigation of the anti-cancerous effect of onion-derived nanovesicles (ODNVs). ODNVs were isolated employing differential centrifugation followed by ultracentrifugation and subsequent characterization using dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM), and Fourier transform infrared spectroscopy (FTIR). Furthermore, we delineated the anti-cancerous effect of ODNVs on two cancer cell line models HeLa (cervical cancer) and PC-3 (prostate cancer) using MTT assay, DAPI-based DNA damage using immunofluorescence microscopy, colony formation assay, migration assay, cell cycle analysis, and evaluation of apoptosis using flow cytometry and western blotting. The findings revealed dose- and time-dependent anti-proliferative effects of ODNVs on both HeLa and PC3 cell lines, accompanied by selective cytotoxicity against cancer cells. Additional results highlighted that ODNVs prevented colony growth and induced S-phase cell cycle arrest. Apoptosis induction was evaluated through alterations in nuclear morphology and the number of apoptotic cells, which increased significantly after ODNV treatment in both cancer cell lines. Furthermore, annexin V/PI staining evaluation of apoptotic cells by flow cytometry demonstrated that ODNV treatment significantly increased the number of apoptotic cells in both PC-3 and HeLa cells. Finally, Western blot analysis indicated changes in apoptosis-related proteins including bcl-2, bax, and caspase-3, emphasizing that the anti-cancerous effect of ODNVs is attributed to the induction of apoptosis and suggests the  unexplored anti-cancerous potential of ODNVs.

This study investigated the bioactive potential of Rhus alata, a plant known for its rich phytochemicals. A previously unreported compound was isolated from R. alata and characterized using various spectroscopic techniques (IR, UV, NMR, MS) and confirmed for the first time by X-ray crystallography. In isolated compound 1, noncovalent interactions between H···H/H···H, C···C/C···C and O···H/H···O play a major role in its packing arrangement. This observation is consistent with the results of Hirshfeld surface analysis, which quantified these interactions as 14.2%, 84.6%, and 1.2%, respectively. The isolated compound was identified as lantabetulic acid (1) (3β,25-expoxy-3α-hydroxylup-20(29)-en-28-oic acid). To understand its potential biological interactions, the binding affinity of lantabetulic acid to biomolecules such as bovine serum albumin (BSA), and human serum albumin (HSA), was assessed. The results showed significant binding efficacy, indicating potential interactions with these molecules. Furthermore, the DPPH assay demonstrated the potent antioxidant activity of this compound. We used in silico molecular docking to clarify the binding affinity between lantabetulic acid and a particular receptor. Furthermore, molecular dynamic simulation studies also explored the binding interaction. As well, MM/GBSA calculations corroborate the simulation results and the stability of the complex. Docking and dynamics studies revealed promising binding scores, suggesting further investigation into their potential therapeutic applications. Geometric parameters and the absorption spectrum of compound 1 were also determined using the DFT approach and compared with experimental findings.

Various important medicines make use of secondary metabolites that are produced by plants. Medicinal plants, such as Withania somnifera and Tinospora cordifolia, are rich sources of chemically active compounds and are reported to have numerous therapeutic applications. The therapeutic use of medicinal plants is widely mentioned in Ayurveda and has folkloric importance in different parts of the world. The aim of this review is to summarize the phytochemical profiles, folkloric importance, and primary pharmacological activity of W. somnifera and T. cordifolia with emphasis on their action against the novel coronavirus.

Medicinal plants are commonly employed mainly due their accessibility, affordability and potency. However, medicinal plants and the associated knowledge are disappearing at an alarming rate due to natural and anthropogenic causes and thus a need for their proper documentation conservation. This study was performed to document traditional knowledge related to use of medicinal plants in Heban-Arsi district, West-Arsi Zone, Oromia Regional State, Ethiopia.

Interviews were conducted with 185 informants to identify medicinal plants used in traditional therapies in the study area. Informant consensus factor (ICF), rank order priority (ROP) values were computed, and preference ranking exercises were performed to assess the relative importance of medicinal plants. Descriptive and inferential statistics were used to measure and compare medicinal plants knowledge between social groups.

A total of 120 medicinal plants were identified for being used to treat different human and animal illnesses in the study area. Most of the medicinal plants (76.4%) were uncultivated ones obtained from different habitats. Leaf was the most frequently used plant part constituting 62.6% of preparations. Oral was the most commonly used route of remedy administration (46%) in treating diseases. Gastrointestinal ailments category had the highest ICF value (0.83). In the study area, the highest rank order priority (ROP) values were recorded for Dombeya torrida (J.F. Gmel.), Artemisia absinthium L., Balanites aegyptiaca (L.) Del., Combretum pisoniiflorum Klotzsch) Engl., Celtis africana Burm. f, Ocimum gratissimum L. and Lagenaria sp. for their uses against snake poison, tuberculosis, liver disorder, stomachache, tuberculosis, febrile illness and liver disorder, respectively, each scoring a value of 100. Significant differences in medicinal plant use knowledge were recorded between male and female informants of different educational level, age and experience. Anthropogenic factors were the primary threats to medicinal plants in the area.

The study area was found to be rich in medicinal plants that are useful in treating a wide range of human and animal illnesses. In future pharmacological and phytochemical investigations, priority needs to be given to medicinal plants of the highest ROP values and those that were reported against ailment categories scoring the highest ICF values.

Triple-negative breast cancer (TNBC) represents a significant global health crisis due to its resistance to conventional therapies and lack of specific molecular targets. This study explored the potential of Eriocephalus racemosus (E. racemosus) as an alternative treatment for TNBC. The cytotoxic properties and high-resolution respirometry mitochondrial activities of E. racemosus against the MDA-MB 231 TNBC cell line were evaluated.

Hexane solvent and bioactive fraction extractions of E. racemosus were performed, while mass spectrometry-based metabolite profiling was used to identify the phytochemical constituents of the extracts. The extracts were further tested against MDA-MB 231 TNBC cells to determine their cytotoxicity. The mode of cell death was determined using flow cytometry. The activities of caspases 3, 8, and 9 were assessed using a multiplex activity assay kit. Glycolytic activity and High-resolution respirometry measurements of mitochondrial function in the MDA-MB 231 cell line were conducted using the Seahorse XFp and Oroboros O2K.

Metabolite profiling of E. racemosus plant crude extracts identified the presence of coumarins, flavonoids, sesquiterpenoids, triterpenoids, and unknown compounds. The extracts demonstrated promising cytotoxic activities, with a half maximal inhibitory concentration (IC50) of 12.84 µg/mL for the crude hexane extract and 15.49 µg/mL for the bioactive fraction. Further, the crude hexane and bioactive fraction extracts induced apoptosis in the MDA-MB-231 TNBC cells, like the reference drug cisplatin (17.44%, 17.26% and 20.25%, respectively) compared to untreated cells. Caspase 3 activities confirmed the induction of apoptosis in both cisplatin and the plant crude extracts, while caspase 8 and 9 activities confirmed the activation of both the intrinsic and extrinsic apoptosis pathways. Increased levels of glycolytic activity were observed in the hexane crude extract. High-resolution respiratory measurements showed elevated mitochondrial activities in all mitochondrial states except for complex-IV activity.

These findings support further exploration of E. racemosus as a potential therapeutic agent for TNBC, offering a promising avenue for the development of targeted treatments with minimal adverse effects.

3,5,7-Trihydroxy-2-phenylchromen-4-one (THF) possesses a diverse range of pharmacological activities. Evidence suggests that THF exerts anticancer activity by distinct mechanisms of action. This study explores the anticancer potential of THF in human lung (A549) and skin (A431) cancer cells by employing different antiproliferative assays. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, neutral red uptake, sulphorhodamine B, and cell motility assays were used to confirm the anticancer potential of THF. Cell target-based and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays were used to explore the effect of THF on the initiation, promotion and progression phase biomarkers of carcinogenesis. THF suppresses the activity of lipoxygenase-5 up to ~40% in both A549 and A431 cells and up to ~50% hyaluronidase activity in A549 cells. qRT-PCR assay reveals that THF inhibits the activity of phosphatidyl inositol-3 kinase/protein kinase B/mammalian target of rapamycin in both cell lines, which is responsible for the initiation of cancer. It also arrests the G2/M phase of the cell cycle in A431 cells and increases the sub-diploid population in both A549 and A431 cell lines which leads to cell death. Annexin V-FITC assay confirmed that THF induces apoptosis and necrosis in A431 and A549 cell lines. Further investigation revealed that THF not only enhances reactive oxygen species production but also modulates mitochondrial membrane potential in both cell lines. It significantly inhibits S-180 tumour formation at 5 and 10 mg/kg bw, i.p. dose. An acute skin toxicity study on mice showed that erythema and edema scores are within the acceptable range, besides acceptable drug-likeness properties and non-toxic effects on human erythrocytes. Conclusively, THF showed potent anticancer activity on skin and lung carcinoma cell lines, suppressed the level of the biomarkers and inhibited tumour growth in mice.

Linalool-rich Rosewood oil (Aniba rosaeodora Ducke) is a natural compound widely used in perfumery industry. Evidence suggests that linalool exerts antidepressant and anxiolytic effects. Conversely, ethanol binge drinking (i.e., intermittent and episodic consumption) during adolescence elicits neurobehavioral alterations associated with brain damage. Here, we investigated whether linalool-rich Rosewood oil administration can improve the emotional and molecular impairments associated with ethanol binge-like exposure during adolescence in female rats. Rosewood oil was obtained by hydrodistillation and posteriorly analyzed. Adolescent female Wistar rats received four-cycles of ethanol binge-like pattern (3 g/kg/day, 3 days on/4 days off) and daily Rosewood oil (35 mg/kg, intranasally) for 28 days. Twenty-four hours after treatments, it was evaluated the impact of ethanol exposure and Rosewood oil treatment on the putative emotional impairments assessed on the splash and forced swimming tests, as well as the levels of brain-derived neurotrophic factor (BDNF), S100B, oxidative parameters, and inflammatory cytokines in prefrontal cortex and hippocampus. Results indicated that Rosewood oil intranasal administration mitigated emotional impairments induced by ethanol exposure accompanied by a marked increase in BDNF, S100B, glutathione (GSH), and antioxidant activity equivalent to Trolox (TEAC) levels in brain areas. Rosewood oil treatment also prevented the ethanol-induced increase of interleukin-1β, interleukin-6, tumor necrosis factor α (TNF-α), and neurofilament light chain (NFL) levels. These findings provide the first evidence that Rosewood oil intranasal administration exerts protective effects against emotional and molecular impairments associated with adolescent ethanol binge-like exposure, possibly due to linalool actions triggering neurotrophic factors, rebalancing antioxidant status, and attenuating proinflammatory process.