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Thaweepanyaporn K, Thompson JB, Vasudevan N, Hawkins JA. Phylogeny, ethnomedicinal use and the distribution of phytoestrogens in the Fabaceae. Front Pharmacol 2025; 16:1583365. [PMID: 40492133 PMCID: PMC12146288 DOI: 10.3389/fphar.2025.1583365] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/25/2025] [Accepted: 05/16/2025] [Indexed: 06/11/2025] Open
Abstract
Introduction Phytoestrogens, with estrogenic activity, are commonly found across the Fabaceae family. Here we develop methods that use phylogeny and ethnomedicinal information in order to identify candidate species for novel phytoestrogens. Method We selected Fabaceae species traditionally used as aphrodisiacs or with applications to control fertility (aphrodisiac-fertility species), to create a cross-cultural dataset of ethnomedicinal use. Using a phylogeny of the Fabaceae, "hot nodes" methods were used to identify lineages with a higher number of species with aphrodisiac-fertility uses. The known distribution of estrogenic flavonoids was used to determine whether the phytoestrogen-containing species was associated with aphrodisiac-fertility "hot nodes". Additionally, we examined the overlap of aphrodisiac-fertility uses with neurological applications, hypothesising that such species may have bioactive compounds with estrogenic properties. Lastly, the "aphrodisiac-fertility hot node" lineages without previously known estrogenic flavonoids were identified. Results We showed species in aphrodisiac-fertility hot nodes were more likely to contain estrogenic flavonoids (21% of species), a major group of phytoestrogens, compared to Fabaceae in the phylogeny (11% of species). Additionally, when aphrodisiac fertility species are limited to those with neurological applications, 62% of the species within hot nodes contain estrogenic flavonoids. Discussion We recognised 43 high-priority hot nodes, these lineages might represent promising targets for future studies on phytoestrogens. The results demonstrated the combining phylogenetic and ethnomedicinal data to guide the discovery of estrogenic flavonoids with therapeutic potential.
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Pandey P, Lakhanpal S, Bishoyi AK, Jyothi SR, Mishra S, Verma M, Singh A, Alam MW, Rab SO, Saeed M, Khan F. Biosynthesis of silver nanoparticles from plant extracts: a comprehensive review focused on anticancer therapy. Front Pharmacol 2025; 16:1600347. [PMID: 40438589 PMCID: PMC12116548 DOI: 10.3389/fphar.2025.1600347] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/26/2025] [Accepted: 04/24/2025] [Indexed: 06/01/2025] Open
Abstract
Cancer is a deadly disease and is one of the primary causes of mortality worldwide. Cancer therapy presents significant challenges, such as chemotherapy resistance, high toxicity, recurrence, and metastasis. As a result, the development of novel therapeutic agents for cancer continues to be a top goal to expand the number of efficient treatments available. The advent of nanotechnology is an important turning point in several scientific disciplines. Owing to the increasing difficulty of this problem, researchers have begun to focus their attention on the possibility of employing plants or extracts from plants as a potential tumor treatment. More than 3,000 medicinal plant species have been documented worldwide for their utilization in cancer treatment. Nevertheless, crude plant extracts lack specificity, and their dosages are not clearly specified. To enhance the therapeutic efficacy of these natural substances, researchers have used them in conjunction with silver nanoparticles (AgNPs). Plants possess intricate phytochemical components including sugars, polyphenols, amino acids, flavonoids, terpenoids, alkaloids, and proteins, which can function as reducing and stabilizing agents. In recent years, the application of plant-derived AgNPs has increased significantly, particularly in cancer treatment. These green-synthesized AgNPs are regarded as outstanding tools for the detection of cancer and targeted drug delivery at the tumor site. By leveraging the distinctive characteristics of nanoparticles and the antioxidant and anticancer qualities of plants, these green-synthesized AgNPs selectively eradicate tumor cells while sparing normal healthy cells. This comprehensive review aimed to summarize the key aspects of plant extracts as anticancer agents, biosynthesis of AgNPs, and recent advancements in the antitumor efficacy of green-synthesized AgNPs.
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Affiliation(s)
- Pratibha Pandey
- Centre for Research Impact and Outcome, Chitkara University Institute of Engineering and Technology, Chitkara University, Rajpura, Punjab, India
| | - Sorabh Lakhanpal
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India
| | - Ashok Kumar Bishoyi
- Marwadi University Research Center, Department of Microbiology, Faculty of Science, Marwadi University, Rajkot, Gujarat, India
| | - S. Renuka Jyothi
- Department of Biotechnology and Genetics, School of Sciences, JAIN (Deemed to be University), Bangalore, Karnataka, India
| | - Swati Mishra
- Department of Pharmacology, IMS and SUM Hospital, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha, India
| | - Meenakshi Verma
- University Centre for Research and Development, Chandigarh University, Mohali, Punjab, India
| | - Ajay Singh
- School of Applied and Life Sciences, Uttaranchal University, Dehradun, India
| | - Mir Waqas Alam
- Department of Physics, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia
| | - Safia Obaidur Rab
- Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia
| | - Mohd Saeed
- Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia
| | - Fahad Khan
- Center for Global Health Research, Saveetha Medical College, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India
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Tocci N, Pramsohler M, Conterno L, Weil T. Hypericum hircinum L.: Botany, Traditional Uses, Phytochemistry, and Pharmacological Properties. PLANTS (BASEL, SWITZERLAND) 2025; 14:1451. [PMID: 40431016 PMCID: PMC12115090 DOI: 10.3390/plants14101451] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/31/2025] [Revised: 05/06/2025] [Accepted: 05/08/2025] [Indexed: 05/29/2025]
Abstract
Hypericum hircinum L., commonly known as goat St. John's wort or stinking tutsan, is a medicinal plant native to the Mediterranean basin and widespread across Europe and parts of the Middle East. It has a long history of traditional uses in folk medicine to treat respiratory diseases, wounds, and burns and to relieve migraine, rheumatism, and muscular pains. Despite numerous scientific studies shading light on the phytochemical profile and on the beneficial properties of the plant extracts, a comprehensive overview of the current knowledge is missing. In this paper, we summarized the available information on botany, traditional uses, phytochemistry, and pharmacological properties of Hypericum hircinum from peer-reviewed articles published till March 2025 in PubMed, ScienceDirect, Wiley, Springer, ACS, Scielo, and Web of Science databases. The presence of numerous valuable compounds, including terpenes, phenolic acids, flavonoids, and phloroglucinols, is reported as well as the wide range of pharmacological properties, such as antimicrobial, antifungal, antiviral, antidepressant, anti-collagenase, anti-α-glucosidase, and antioxidant activities, together with non-pharmacological properties. The data reported in this review contribute to a deeper understanding of the biological properties of the species and pave the way for further investigation of its potential applications.
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Affiliation(s)
- Noemi Tocci
- Laimburg Research Centre, Pfatten/Vadena, 39040 Auer/Ora, Italy; (M.P.); (L.C.)
| | - Manuel Pramsohler
- Laimburg Research Centre, Pfatten/Vadena, 39040 Auer/Ora, Italy; (M.P.); (L.C.)
| | - Lorenza Conterno
- Laimburg Research Centre, Pfatten/Vadena, 39040 Auer/Ora, Italy; (M.P.); (L.C.)
| | - Tobias Weil
- Research and Innovation Centre, Fondazione Edmund Mach, 38098 San Michele all’Adige, Italy;
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G MR, D DW, V J, N B K, M S, Vijay N, V M BG. Different in vivo administration routes of essential oil for various therapies: A review. Fitoterapia 2025; 184:106577. [PMID: 40311706 DOI: 10.1016/j.fitote.2025.106577] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/10/2025] [Revised: 04/12/2025] [Accepted: 04/27/2025] [Indexed: 05/03/2025]
Abstract
Essential oils derived from aromatic and non-aromatic plant sources, are recognized for their in vivo potential therapeutic benefits, including anti-oxidant, anti-inflammatory, anti-cancer, immunomodulatory and other regulatory roles by the scientific community. However, translation of such in vivo therapeutic application in clinical field is not yet available. This study examines the pre-clinical scientific studies on varied administration methods of essential oils for therapeutic purposes. The focus of this study is to explore the different administration routes, which are crucial for optimizing the delivery of these bioactive compounds for internal administration and improving their therapeutic effectiveness. Traditional and innovative methods, such as inhalation, dermal application, oral ingestion, intraperitoneal injection, and intravenous infusion are investigated. Each route has its advantages and challenges, which may impact the bioavailability, systemic distribution and toxicity of essential oils. However, such comparative pharmacokinetic and toxicity studies are lacking for the essential oils. The available basic information regarding in vivo bioavailability and toxic reactions of essential oils are discussed briefly in this review. The research findings have significant implications for developing targeted and personalized essential oil treatments, advancing integrative medicine and complementary therapy approaches. The analysis of various in vivo therapeutic study findings implies that a promising therapeutic efficiency can be achieved with essential oils at different dose ranges for different routes of administrations. The comparative analysis on therapeutic effects of different routes of administration and methods essential oil extraction are lacking. The bioavailability and toxicity studies are also limited for these therapeutic studies. Furthermore, the detailed molecular mechanism of in vivo therapeutic actions is not yet established in the studies. Ultimately, this investigation adds to the expanding knowledge base on essential oil administration routes, providing valuable guidance for healthcare professionals and researchers in the field of natural medicine. However, extensive in vivo pre-clinical studies are warranted for translation of essential oil therapy to clinical usage. Also all the traditional uses of essential oil for therapy must be validated via scientific studies.
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Affiliation(s)
- Monith Raj G
- Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore 641114, Tamil Nadu, India
| | - David Wilson D
- School of Sciences, Arts and Media, Karunya Institute of Technology and Sciences, Coimbatore 641114, Tamil Nadu, India
| | - Jenitha V
- Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore 641114, Tamil Nadu, India
| | - Kokelavani N B
- Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore 641114, Tamil Nadu, India
| | - Srividhya M
- Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore 641114, Tamil Nadu, India
| | - Nandana Vijay
- Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore 641114, Tamil Nadu, India
| | - Berlin Grace V M
- Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore 641114, Tamil Nadu, India.
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Khan G, Hussain MS, Sultana A, Khan Y, Fatima R, Maqbool M, Bisht AS. Unveiling the Therapeutic Potential of Rumex abyssinicus: Phytochemical Insights and Pharmacological Applications. CURRENT PHARMACOLOGY REPORTS 2025; 11:32. [DOI: 10.1007/s40495-025-00411-4] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Accepted: 04/10/2025] [Indexed: 05/04/2025]
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Qureshi KA, Parvez A, Ismatullah H, Almahasheer H, Al Rugaie O. Exploring the antimicrobial and antibiofilm potency of four essential oils against selected human pathogens using in vitro and in silico approaches. PLoS One 2025; 20:e0315663. [PMID: 40273059 DOI: 10.1371/journal.pone.0315663] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/30/2024] [Accepted: 11/28/2024] [Indexed: 04/26/2025] Open
Abstract
Multi-drug-resistant (MDR) pathogens pose a significant global health challenge, underscoring the urgent need for novel antimicrobial agents with minimal toxicity to humans. This study investigated the in vitro and in silico antimicrobial and antibiofilm potentials of four essential oils (EOs): clove bud oil (CBO; Syzygium aromaticum L.), black seed oil (BSO; Nigella sativa L.), cinnamon bark oil (CNBO; Cinnamomum zeylanicum), and citronella oil (CTLO; Cymbopogon nardus L.), against 19 selected human pathogens, including MDR strains. Among the tested EOs, CBO, BSO, and CNBO exhibited the highest antibacterial activity against Staphylococcus epidermidis, with the mean zone of inhibition diameters (ZIDs) of 20.0 ± 0.2 mm, 46.0 ± 0.3 mm, and 32.0 ± 0.1 mm, respectively, at a concentration of 10 µL/disc, while CTLO displayed no antibacterial activity. CNBO demonstrated superior antifungal activity, with the mean ZIDs of 49.0 ± 0.3 mm and 36.0 ± 0.3 mm for Candida albicans and Aspergillus niger, respectively. Molecular docking analyses revealed robust interactions of key bioactive compounds-eugenol (EU) from CBO, thymoquinone (TQ) from BSO, cinnamaldehyde (CN) from CNBO, citronellal (CIT) and linalool (LIN) from CTLO-with microbial target proteins, substantiating their antimicrobial and antibiofilm potential. Notably, CTLO, despite limited in vitro activity, exhibited unique binding interactions in silico, suggesting potential niche applications. These findings underscore the translational potential of EOs as alternative antimicrobial therapies against MDR infections, particularly biofilm-associated infections, and highlight the need for further in vivo studies to validate their efficacy and safety.
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Affiliation(s)
- Kamal A Qureshi
- Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraydah, Saudi Arabia
| | - Adil Parvez
- NextGen Life Sciences Pvt. Ltd., New Delhi, India
| | - Humaira Ismatullah
- School of Interdisciplinary Engineering & Sciences (SINES), National University of Sciences & Technology (NUST), Islamabad, Pakistan
| | - Hanan Almahasheer
- Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University (IAU), Dammam, Saudi Arabia
| | - Osamah Al Rugaie
- Department of Biology and Immunology, College of Medicine, Qassim University, Buraydah, Saudi Arabia
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Vu TTL, Wang SL, Luc QT, Do TL, Dam TBH, Phan TKP, Nguyen TT, Tran PH, Nguyen AD, Nguyen VB. New Record of Potential Tyrosinase Inhibitory, Antioxidant, and Photoprotective Effects of Vietnamese Castanea mollissima Extracts. Chem Biodivers 2025:e202500994. [PMID: 40267250 DOI: 10.1002/cbdv.202500994] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/20/2025] [Accepted: 04/23/2025] [Indexed: 04/25/2025]
Abstract
In this study, the potential anti-tyrosinase, antioxidant, and ultraviolet (UV) absorption efficiency of the extracts of different parts of Castanea mollissima were evaluated. The leaf extract exhibited the most potential antioxidant effect with 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging abilities of >90%. C. mollissima extracts showed high UV-A and UV-B absorption efficiency. In addition, screening of the leaf extract demonstrated C. mollissima leaves as a rich source of tyrosinase inhibitors with maximum inhibitory activity and half-maximal inhibitory concentration values of 96% and 336.43 µg/mL, respectively. This is the first-ever report of screening of leaf extract for its potential activities. Eighteen phyto-compounds were identified from the C. mollissima leaf extract using gas chromatography-mass spectrometry and high-performance liquid chromatography techniques. Of these, the content of compounds 10, 11, 12, 13, 14, and 16 in the C. mollissima leaf extract was significantly high (14.35-88.42 µg/g). The molecular docking, absorption, distribution, metabolism, excretion, and toxicity, and Lipinski's rule of five analyses indicated high interaction efficacy of all the major compounds (docking score values of -6.75 to -8.89 kcal/mol) with the target enzyme tyrosinase, had drug properties, and were non-toxic for human use.
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Affiliation(s)
- Thi Thu Le Vu
- Thai Nguyen University of Agriculture and Forestry (TNU), Thai Nguyen, Vietnam
| | - San-Lang Wang
- Department of Chemistry, Tamkang University, New Taipei City, Taiwan
| | - Quang Tan Luc
- Thai Nguyen University - Ha Giang Campus, Ha Giang, Vietnam
| | - Tien Lam Do
- Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam
- Faculty of Chemistry, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
| | - Thi Bich Hanh Dam
- Graduate University of Science and Technology, Hanoi, Vietnam
- Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot, Vietnam
| | - Thi Kim Phung Phan
- Faculty of Medicine and Pharmacy, Tay Nguyen University, Buon Ma Thuot, Vietnam
| | - Thanh Thao Nguyen
- Faculty of Medicine and Pharmacy, Tay Nguyen University, Buon Ma Thuot, Vietnam
| | - Phuc Hoang Tran
- Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam
| | - Anh Dzung Nguyen
- Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, Vietnam
| | - Van Bon Nguyen
- Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, Vietnam
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Liao Q, Zhao W, Wang Z, Xu L, Yang K, Liu X, Zhang L. Deciphering metabolic disease mechanisms for natural medicine discovery via graph autoencoders. Front Pharmacol 2025; 16:1594186. [PMID: 40337507 PMCID: PMC12055761 DOI: 10.3389/fphar.2025.1594186] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/15/2025] [Accepted: 04/08/2025] [Indexed: 05/09/2025] Open
Abstract
Metabolic diseases, such as diabetes, pose significant risks to human health due to their complex pathogenic mechanisms, complicating the use of combination drug therapies. Natural medicines, which contain multiple bioactive components and exhibit fewer side effects, offer promising therapeutic potential. Metabolite imbalances are often closely associated with the pathogenesis of metabolic diseases. Therefore, metabolite detection not only aids in disease diagnosis but also provides insights into how natural medicines regulate metabolism, thereby supporting the development of preventive and therapeutic strategies. Deep learning has shown remarkable efficacy and precision across multiple domains, particularly in drug discovery applications. Building on this, We developed an innovative framework combining graph autoencoders (GAEs) with non-negative matrix factorization (NMF) to investigate metabolic disease pathogenesis via metabolite-disease association analysis. First, we applied NMF to extract discriminative features from established metabolite-disease associations. These features were subsequently integrated with known relationships and processed through a GAE to identify potential disease mechanisms. Comprehensive evaluations demonstrate our method's superior performance, while case studies validate its capability to reveal pathological mechanisms in metabolic disorders including diabetes. This approach may facilitate the development of natural medicine-based interventions. Our data and code are available at: https://github.com/Lqingquan/natural-medicine-discovery.
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Affiliation(s)
- Qingquan Liao
- Department of Information Technology, Hunan Police Academy, Changsha, China
| | - Wei Zhao
- Department of Information Technology, Hunan Police Academy, Changsha, China
| | - Zhan Wang
- School of Electronic and Communication Engineering, Shenzhen Polytechnic University, Shenzhen, China
| | - Lei Xu
- School of Electronic and Communication Engineering, Shenzhen Polytechnic University, Shenzhen, China
| | - Kun Yang
- School of Data Science and Artificial Intelligence, Wenzhou University of Technology, Wenzhou, China
| | - Xinxin Liu
- School of Data Science and Artificial Intelligence, Wenzhou University of Technology, Wenzhou, China
| | - Lichao Zhang
- School of Intelligent Manufacturing and Equipment, Shenzhen Institute of Information Technology, Shenzhen, China
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Tinkov AA, Korobeinikova TV, Baranova OV, Chang JS, Chao JCJ, Rylina EV, Prakash NT, Nemereshina ON, Skalny AV. Trace Element and Mineral Content in Medicinal Plants Collected in the Altai Mountains Foothills. Biol Trace Elem Res 2025:10.1007/s12011-025-04620-2. [PMID: 40257550 DOI: 10.1007/s12011-025-04620-2] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 02/18/2025] [Accepted: 04/07/2025] [Indexed: 04/22/2025]
Abstract
The objective of the present study was to assess the patterns of trace element and mineral accumulation in 21 medicinal plants collected in the Altai Mountains foothills. The levels of trace elements and minerals in Hippophae rhamnoides, Aronia melanocarpa, Sorbus aucuparia, Viburnum opulus, Rosa canina, Crataegus sanguinea (fruits), Chamaenerion angustifolium, Echinacea purpurea, Fagopyrum esculentum, Trifolium pratense, Origanum vulgare, Achillea millefolium, Mentha piperita (herbs), Plantago major, Salvia officinalis, Urtica dioica (leaves), Panax ginseng, Rhaponticum carthamoides, Eleuterococcus senticosus, and Taraxacum officinale (roots), and Matricaria recutita flowers were assessed using inductively-coupled plasma mass-spectrometry. Comparative analysis revealed that R. carthamoides roots accumulated the highest level of toxic trace elements (Al, Cd, Ni, Pb). C. angustifolium herbs and V. opulus fruits are characterized by the highest As and Sn levels, respectively. The highest content of essential trace elements and minerals was observed in H. rhamnoides fruits (Zn), V. opulus fruits (Cu), E. purpurea herbs (I, Mg), R. carthamoides roots (Fe), O. vulgare herbs (Mn), and M. recutita herbs (Se). In turn, R. canina fruits are characterized by the lowest levels of certain essential and toxic trace elements. Significant differences in B, Cr, Li, Si, and Sr content were also revealed. Discriminant analysis revealed complete discrimination of certain medicinal plants based on their trace element and mineral content. The obtained data indicate the potential usefulness of medicinal plants as sources of certain essential trace elements and minerals, as well as provides data for risk assessment of toxic trace element intake following administration of medicinal plants.
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Affiliation(s)
- Alexey A Tinkov
- Laboratory of Ecobiomonitoring and Quality Control, Yaroslavl State University, Yaroslavl, 150003, Russia
- Center of Bioelementology and Human Ecology, IM Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119146, Russia
- Department of Medical Elementology, Peoples' Friendship, University of Russia, RUDN University), Moscow, 117198, Russia
| | - Tatiana V Korobeinikova
- Center of Bioelementology and Human Ecology, IM Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119146, Russia
- Department of Medical Elementology, Peoples' Friendship, University of Russia, RUDN University), Moscow, 117198, Russia
| | - Oksana V Baranova
- Institute of Bioelementology, Orenburg State University, Orenburg, 460018, Russia
| | - Jung-Su Chang
- School of Nutrition and Health Sciences, and, Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition , Taipei Medical University, Taipei, 11031, Taiwan
- Nutrition Research Center, Taipei Medical University Hospital, Taipei, 11031, Taiwan
| | - Jane C-J Chao
- School of Nutrition and Health Sciences, and, Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition , Taipei Medical University, Taipei, 11031, Taiwan
- Nutrition Research Center, Taipei Medical University Hospital, Taipei, 11031, Taiwan
| | - Elena V Rylina
- Department of Medical Elementology, Peoples' Friendship, University of Russia, RUDN University), Moscow, 117198, Russia
| | - N Tejo Prakash
- School of Energy and Environment, Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, India
| | - Olga N Nemereshina
- Department of Biochemistry, Orenburg State Medical University, Orenburg, 460018, Russia
| | - Anatoly V Skalny
- Center of Bioelementology and Human Ecology, IM Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119146, Russia.
- Department of Medical Elementology, Peoples' Friendship, University of Russia, RUDN University), Moscow, 117198, Russia.
- Institute of Bioelementology, Orenburg State University, Orenburg, 460018, Russia.
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Adebayo OT, Oluremi BB, Ogunlakin AD, Gyebi GA, Sonibare MA. Pharmacognostic evaluation and antimicrobial activity of Pteridium aquilinum (L.) Kuhn leaves (Onocleaceae) via in vitro and in silico perspectives. PLoS One 2025; 20:e0318943. [PMID: 40203065 PMCID: PMC11981126 DOI: 10.1371/journal.pone.0318943] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/10/2024] [Accepted: 01/21/2025] [Indexed: 04/11/2025] Open
Abstract
BACKGROUND AND OBJECTIVE Traditionally, Pteridium aquilinum L. has been utilized as medicine for ages, however, it is not listed in the Nigerian herbal pharmacopeia, and there is no information regarding its standardization and antimicrobial activity. Therefore, the purpose of this study was to examine the pharmacognostic parameters and antimicrobial activity of Pteridium aquilinum leaf. METHODS Macroscopy, chemo-microscopy, fluorescence, and microscopic analyses of the leaf were investigated using standard methods. Qualitative and quantitative phytochemical screening, thin layer chromatography (TLC), GC-MS, and FTIR were also determined using standard procedures. Antioxidants were evaluated using DPPH. The antimicrobial activities of methanol extract and fractions were evaluated using Agar well diffusion method against Candida albicans, Aspergillus niger, Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, and Pseudomonas aeruginosa. The macroscopic features of P. aquilinum leaf include a bi-pinnate leaflet and alternate pinna arrangement. The GC-MS-identified compounds in the most active (DCM fraction) were docked against Candida albicans Sterol 14-alpha demethylase (5TZ1) and Escherichia coli DNA gyrase subunit B (6YD9). RESULTS The macroscopic features and microscopic features such as anomocytic stomata, numerous stomata in the abaxial layer, and absence of stomata in the adaxial layer were observed. Chemomicroscopy of the powdered leaves shows that the leaf contains tannins, starch, and lignin. GC-MS detected eighteen compounds. The antimicrobial test revealed that the dichloromethane fraction of P. aquilinum leaf was most active on all the test strains (bacteria and fungi) at 25 mg/mL to 100 mg/mL concentrations. Through in silico research, the binding of 1,2-benzenedicarboxylic acid, (4-hydroxybenzoyl) hydrazine, octadecadienoyl chloride, and 11,14-Eicosadienoic acid, detected in the DCM fraction by GC-MS analysis, to the active sites of 5TZ1 and 6YD9 was stable. CONCLUSION This research gave scientific credence to the traditional medical practice of treating infections with P. aquilinum leaves.
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Affiliation(s)
| | - Bolaji Bosede Oluremi
- Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria
| | - Akingbolabo Daniel Ogunlakin
- Department of Biochemistry, Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Bowen University, Iwo, Nigeria
| | - Gideon Ampoma Gyebi
- Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, Durban, South Africa
| | - Mubo Adeola Sonibare
- Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria
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Bouafia A, Laib I, Laouini SE, Azzi M, Alharthi F, Souwaileh AA, Abdullah JAA. Comprehensive Bioactivities and Phytochemical Profiling of Rumex vesicarius: Antioxidant Potential, Anti-Diabetic Properties, and Anti-Biofilm Effects Under Thermal Treatment. Chem Biodivers 2025:e202500329. [PMID: 40193246 DOI: 10.1002/cbdv.202500329] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/25/2025] [Revised: 03/18/2025] [Accepted: 04/07/2025] [Indexed: 04/09/2025]
Abstract
This study investigates the effect of thermal treatment on the phytochemical composition and bioactivities of Rumex vesicarius, focusing on its antioxidant, antidiabetic, and anti-biofilm properties. Quantitative analysis showed that at ambient temperature, the extract had the highest phenolics (57.89 mg GAE/g), flavonoids (19.45 mg QE/g), tannins (12.78 mg CE/g), flavonols (6.48 mg), and anthraquinones (2.078 mg). At 60°C, it retained significant phenolics (52.89 mg GAE/g) and flavonoids (18.45 mg QE/g) with minimal degradation. At 90°C, phenolics decreased slightly (43.59 mg GAE/g), but enzymatic inhibition and antimicrobial properties improved. β-Carotene stability varied, with untreated extract at a minimum inhibitory concentration (IC50) of 118.136 µg/mL. Peak antioxidant activity was at 150°C (IC50 = 102.77 µg/mL), with degradation above 150°C. Antidiabetic potential, via α-amylase and α-glucosidase inhibition, showed the lowest IC50 values (92.106 µg/mL for both) at 90°C. Beyond 120°C, IC50 rose to 268.35 µg/mL (α-amylase) and 268.31 µg/mL (α-glucosidase) at 210°C. Anti-biofilm activity peaked at 90°C (IC50 = 33.55 µg/mL), with untreated and 60°C extracts showing strong inhibition (∼80%-90%). Above 90°C, inhibition dropped, reaching IC50 of 253.53 µg/mL at 210°C. Moderate heating (60-90°C) optimizes bioactive availability, positioning R. vesicarius for pharmaceutical and nutraceutical applications.
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Affiliation(s)
- Abderrhmane Bouafia
- Department of Process Engineering and Petrochemical, Faculty of Technology, University of El Oued, El Oued, Algeria
- BBCM Laboratory, Faculty of Technology, University of El Oued, El Oued, Algeria
| | - Ibtissam Laib
- Department of Cellular and Molecular Biology, Faculty of Natural and Life Sciences, University of El Oued, El Oued, Algeria
- Laboratory of Biodiversity and Biotechnology Applications in Agriculture, University of El Oued, El Oued, Algeria
| | - Salah Eddine Laouini
- Department of Process Engineering and Petrochemical, Faculty of Technology, University of El Oued, El Oued, Algeria
- BBCM Laboratory, Faculty of Technology, University of El Oued, El Oued, Algeria
| | - Manel Azzi
- Faculty of Natural and Life Sciences, Laboratory of Biology, Environment and Health, University of El Oued, El Oued, Algeria
| | - Fahad Alharthi
- Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia
| | - Abdullah Al Souwaileh
- Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia
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12
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Sinze Metiave AA, Tedonkeu AT, Tamokou JDD, Nanfack ARD, Matsuete-Takongmo G, Kamtcha Wetadieu D, Tsopmo A, Tene M. Antibacterial stigmastane-type steroids and other constituents from the leaves of Vernonia glabra (Steetz) Vatke (Asteraceae). Nat Prod Res 2025; 39:1809-1823. [PMID: 37955140 DOI: 10.1080/14786419.2023.2278756] [Citation(s) in RCA: 1] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/06/2023] [Revised: 10/16/2023] [Accepted: 10/25/2023] [Indexed: 11/14/2023]
Abstract
Two new stigmastane steroids (1 and 2) were isolated from the methanol extract of the leaves of Vernonia glabra, together with seventeen known compounds (3-19) including one fatty acid, four triterpenoids, four steroids, one trinitropropanoyl glucoside, and seven flavonoids. The structures of compounds 1 and 2 were assigned based on their IR, NMR and MS data, and by comparison with literature values. The MeOH extract, its fractions and isolated compounds were subjected to in vitro antibacterial assay against two Gram-positive (Staphylococcus aureus ATCC25923 and Streptococcus pneumoniae ATCC49619) and two Gram-negative (Escherichia coli ATCC8739 and Klebsiella pneumoniae ATCC10031) bacteria, using broth microdilution method. The extract and fractions exhibited (16 ≤ MIC ≤ 512 μg/mL) antibacterial activities. The isolated and tested compounds were also active (16 ≤ MIC ≤ 128 μg/mL) against the four pathogenic bacteria, with compound 2 being the most active and E. coli, the most sensitive microorganism.
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Affiliation(s)
- Audrey A Sinze Metiave
- Natural Products Chemistry Research Unit, Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
| | - Alex Tchinda Tedonkeu
- Natural Products Chemistry Research Unit, Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
| | - Jean-De-Dieu Tamokou
- Research Unit of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
| | - Arno R Donfack Nanfack
- Natural Products Chemistry Research Unit, Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
| | - Germaine Matsuete-Takongmo
- Research Unit of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
| | - Duplex Kamtcha Wetadieu
- Natural Products Chemistry Research Unit, Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
| | - Apollinaire Tsopmo
- Food Science Program, Department of Chemistry, and Institute of Biochemistry, Carleton University, Ottawa, Canada
| | - Mathieu Tene
- Natural Products Chemistry Research Unit, Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
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13
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Goswami MJ, Chakrabarty R, Das B, Kakati D. Isolation and Characterisation of Bioactive Steroidal Compounds From the Leaf Extract of Sarcochlamys pulcherrima. Chem Biodivers 2025:e202500100. [PMID: 40122691 DOI: 10.1002/cbdv.202500100] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/09/2025] [Revised: 03/20/2025] [Accepted: 03/21/2025] [Indexed: 03/25/2025]
Abstract
Sarcochlamys pulcherrima (Roxb.) Gaudich is a small, traditionally used medicinal plant belonging to the Urticaceae family. The leaves of the plant are commonly consumed by different tribes inhabiting the northeastern states of India. This work involves dereplicating ethanolic leaf extract of S. pulcherrima, leading to the isolation of two new steroidal compounds-SP-C1 and SP-C2-characterised by single crystal x-ray diffraction analysis. The compound SP-C2 displayed significant antioxidant properties with an IC50 value of 4.75 ± 0.22 µg mL-1 in the 2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging assay along with ferric reducing antioxidant power (FRAP) value of 1792.40 ± 0.11 µM, which is comparable to that of the employed standard antioxidant ascorbic acid (2032.60 ± 0.12 µM). SP-C2 also exhibited significant α-glucosidase inhibitory potential with an IC50 value of 506.53 ± 0.24 µg mL-1 in close resemblance with that of the standard α-glucosidase inhibitor acarbose (420.32 ± 0.62 µg mL-1).
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Affiliation(s)
| | | | - Babulal Das
- Central Instrument Facility, Indian Institute of Technology Guwahati, Guwahati, India
| | - Dwipen Kakati
- Department of Chemistry, Rajiv Gandhi University, Itanagar, India
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Joshi V, Bachhar V, Mishra SS, Shukla RK, Kumar N, Duseja M. Identification of Potential Anticancer Phytochemicals of Piper chaba by Comprehensive Molecular Docking, Molecular Dynamics Simulation and In-Vitro Studies. Chem Biodivers 2025:e202403269. [PMID: 40101115 DOI: 10.1002/cbdv.202403269] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/10/2024] [Revised: 03/06/2025] [Accepted: 03/07/2025] [Indexed: 03/20/2025]
Abstract
Human colon cancer and prostate cancer are the most common malignancy globally. Despite the availability of many treatments, resistance to traditional medicines, such as chemotherapy and radiotherapy, remains a severe obstacle in cancer treatment. Hence, searching for new therapeutic options is of utmost priority. The present investigation evaluates the in-silico and in-vitro anticancer potential of phytochemicals of Piper chaba. The cytotoxicity results demonstrated that the plant extract exhibited significant anticancer activity, with IC50 values of 12.66 ± 0.25 µg/mL for HCT-116 and 19.49 ± 0.37 µg/mL for DU-145. Molecular docking and molecular dynamics simulations were performed to explore the interaction of potential drug targets with the phytochemicals. Subsequently, pharmacokinetic parameters calculations were performed to evaluate the drug-likeness. Piperine exhibits the highest binding affinity for vascular endothelial growth factor receptor-2 (VEGFR2) protein with a docking score of -9.71 kcal/mol. Sylvatine had a greater binding affinity for human epidermal growth factor receptor 3 (HER3) protein than the other phytochemicals. Isopiperine, chabamide, Piperlonguminine, Santamarine and Versalide only exhibited ligand activity for human IKK beta protein (inhibitor of kappa B kinase). Additionally, a principal component analysis was performed to strengthen the investigation's scope. These proposed phytochemicals are reported to possess potential VEGFR2, HER2 and human IKK beta inhibition. The best phytochemical hits have excellent binding affinity and hold a massive anticancer potential, opening up new avenues for prospective future investigations in cancer treatment.
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Affiliation(s)
- Vibha Joshi
- Department of Chemistry, Analytical Chemistry Lab, School of Physical Sciences, DIT University, Dehradun, India
| | - Vishwajeet Bachhar
- Department of Chemistry, Analytical Chemistry Lab, School of Physical Sciences, DIT University, Dehradun, India
| | - Shashank Shekher Mishra
- School of Pharmaceutical and Population Health Informatics, Faculty of Pharmacy, DIT University, Dehradun, India
| | - Ravi K Shukla
- Department of Physics, Advanced Functional Smart Materials Laboratory, School of Physical Sciences, DIT University, Dehradun, India
| | - Neeraj Kumar
- Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Bhupal Nobles' University, Udaipur, India
| | - Manisha Duseja
- Department of Chemistry, Analytical Chemistry Lab, School of Physical Sciences, DIT University, Dehradun, India
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Lulesa F, Alemu S, Kassa Z, Awoke A. Ethnobotanical investigation of medicinal plants utilized by indigenous communities in the Fofa and Toaba sub-districts of the Yem Zone, Central Ethiopian Region. JOURNAL OF ETHNOBIOLOGY AND ETHNOMEDICINE 2025; 21:14. [PMID: 40045312 PMCID: PMC11884170 DOI: 10.1186/s13002-025-00768-2] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 01/12/2025] [Accepted: 02/13/2025] [Indexed: 03/09/2025]
Abstract
BACKGROUND Ethnobotany investigates the ways in which communities utilize plant species to tackle a range of health concerns in both humans and animals, highlighting the intricate relationships between plant life and local cultural practices. The degradation of habitats resulting from agricultural development and deforestation poses a considerable risk to the accessibility of these vital plants. This research was conducted in the Fofa and Toba sub-districts of the Yem Zone in Central Ethiopia, aiming primarily to explore and record the medicinal plant species that indigenous communities use for treating various health issues in both people and livestock. METHODS This study was carried out between March 2023 and April 2024, involving a total of 96 informants, with 12 selected from each kebele. A range of quantitative methodologies were employed in the research, such as the informant consensus factor (ICF), fidelity level (FL), plant part value, preference ranking, and direct matrix ranking. Furthermore, various statistical analyses including independent t-tests, one-way ANOVA, correlation, and regression were performed using R to evaluate and compare the ethnobotanical knowledge among different groups of informants. RESULTS A total of 164 medicinal plant species from 140 genera and 60 families were identified in the study. Of these species, 67.68% were utilized for human ailments, 19.5% for livestock issues, and 12.8% for both. The sources of these medicinal plants included 81 species from wild areas, 35 from home gardens, 23 from agricultural fields, 15 from living fences, and 10 from roadsides. In terms of growth forms, herbs comprised the largest group with 76 species, followed by trees with 42 species, shrubs with 34 species, and climbers with 9 species. The most commonly used plant parts were leaves, followed by roots. Preparations were primarily made by crushing the plants, with other methods including powdering, chewing, smashing, and boiling. The highest informant consensus factor value of 91% indicated a significant healing potential for respiratory diseases, common colds, coughs, and fevers. Notably, A. sativum (for malaria) and H. rueppelii (for abdominal pain) recorded the highest fidelity level values. Additionally, the average number of medicinal plants reported by participants varied significantly across different demographic factors, including gender, age groups, educational levels, and experience (P < 0.05). CONCLUSION The study area is home to a wide variety of potential medicinal plants along with valuable indigenous knowledge. To address the growing anthropogenic threats and safeguard these plants and their associated knowledge, it is essential to adopt effective conservation strategies and promote responsible use. Additionally, the medicinal properties of these plants should be scientifically validated to harmonize local knowledge with modern medicine effectively.
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Affiliation(s)
- Firehun Lulesa
- Department of Biology, Wolkite University, P.O.Box:07, Wolkite, Ethiopia
| | - Shiferaw Alemu
- Department of Biology, Wolkite University, P.O.Box:07, Wolkite, Ethiopia
| | - Zewdie Kassa
- Department of Biology, Salale University, P.O.Box: 245, Fiche, Ethiopia.
| | - Ashebir Awoke
- Department of Biology, Mizan-Tepi University, P.O.Box: 260, Mizan Teferi, Ethiopia
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Dembele N, Somboro AA, Traore N, Badiaga M, Cisse S, Cisse M, Nick P. Anti-microtubular activity of total alkaloids and aqueous extract of Detarium microcarpum a medicinal plant harvested in Mali. PROTOPLASMA 2025; 262:415-430. [PMID: 39505773 PMCID: PMC11839714 DOI: 10.1007/s00709-024-02003-3] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/19/2024] [Accepted: 10/20/2024] [Indexed: 11/08/2024]
Abstract
Detarium microcarpum, is a species confined to drier regions of west and central Africa used to treat various diseases including cancer. Phytochemical screening revealed the presence of secondary metabolites (alkaloids) The aim of this work is to study the effect of total aqueous extracts and alkaloid fractions from D. microcarpum leaves, bark and roots on Nicotiana tabacum L. cv. 'Bright Yellow 2' (BY-2) tobacco cell line GFP-TuA3 expressing a N-terminal fusion of GFP. The plant was harvested in two different regions of Mali with a contrasting climate. The effects of the extracts on the microtubules was followed by spinning disc confocal microscopy. We showed that the anti-microtubular effect of the extracts is dose-dependent, depends of the sampling site and the part of the plant used. Total alkaloids extracted of D. microcarpum bark have more effect on microtubules than leaf and root. The bioactivity-guided fractionation should be used to screen out the biologically active compounds of the total alkaloid extracts of the bark of D. microcarpum.
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Affiliation(s)
| | | | - Nah Traore
- Faculty of Sciences and Techniques, USTTB, Bamako, Mali
| | | | | | - Mody Cisse
- Faculty of Pharmacy, USTTB, Bamako, Mali
| | - Peter Nick
- Karlsruhe Institute for Technologies, KIT, Karlsruhe, Germany
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17
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Salimi A, Hamzeloo-Moghadam M, Irani M, Esmaeili S. Antimalarial evaluation of selected medicinal plants traditionally used for treatment of fever, by inhibition test of heme polymerization. ANNALES PHARMACEUTIQUES FRANÇAISES 2025; 83:311-321. [PMID: 39413969 DOI: 10.1016/j.pharma.2024.10.003] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/24/2024] [Revised: 09/30/2024] [Accepted: 10/09/2024] [Indexed: 10/18/2024]
Abstract
OBJECTIVES World Health Organization (WHO) has reported 249 million cases infected by malaria worldwide and 608 thousands deaths in 2022. Investigations for new antimalarial drugs from traditional medicine have proven to be more effective and less expensive. The medicinal plants that have been used for treatment of malaria, generally known as types of fevers in traditional medicine, can be suitable candidates for evaluating antimalarial effects. The aim of the present study was to evaluate the in vitro mechanism of antimalarial action of selected medicinal plants by inhibition test of heme detoxification (ITHD). MATERIAL AND METHODS The methanol extract and fractions were prepared through maceration of the dry powdered plants. The ITHD method was carried in 96-wells plate and the percentage of heme polymerization inhibition was determined. The cytotoxicity of the effective plants was examined on MDBK cell line by MTT assay. Bioassay guided fractionation was performed exposing to size exclusion column chromatography and liquid-liquid fractionation and was assayed by the ITHD method for the sample with the least IC50 value and lowest cytotoxic effect. RESULTS The methanol fraction of Viola odorata whole plant showed the most considerable results among the tested plants with IC50 171.8μg.mL-1 beside the lowest cytotoxic effects. This fraction by the bioassay guided fractionation led to fraction SB2 and this fraction demonstrated the most effective result with lowest IC50=14.8±3μg.mL-1 in ITHD assay. CONCLUSION Regarding the results of the present study and the traditional use of V. odorata for overcoming fever in Iranian traditional medicine, the final fraction of the plant could be proper candidate for further phytochemical and antimalarial studies.
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Affiliation(s)
- Arian Salimi
- Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
| | - Maryam Hamzeloo-Moghadam
- Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Mahboobeh Irani
- Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Somayeh Esmaeili
- Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
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18
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Nyila MA. Ximenia caffra Sond. the magic wild indigenous plant that offers immense contribution as food and medicine. Nat Prod Res 2025:1-13. [PMID: 40012125 DOI: 10.1080/14786419.2025.2471827] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/18/2024] [Revised: 01/07/2025] [Accepted: 02/14/2025] [Indexed: 02/28/2025]
Abstract
The wild indigenous African tree, Ximenia caffra Sond also commonly known as 'sour plum' and found in Southern Africa is traditionally used as a source of food and medicine by rural communities. Its fruit has been found to have vitamins, minerals, macronutrients, other important compounds such as phenolics and flavonoids. Other parts of the plant such as the seeds, roots and the leaves are used to treat vast different ailments such as cough, cancer, sexual transmitted disease and so on. Its polyphenols compounds that contribute to its antioxidant, anti-inflammatory, anticancer and antimicrobial activities. The aim of this review is to explore X. caffra, the indigenous fruit that has health benefit of nutraceuticals and medicinal food, therapeutic capabilities in traditional medicine. The words' Ximenia cafrra' were used. The juice of X. caffra fruits comprises several potentially beneficial phytochemicals, minerals, and initial assessment indicate a remarkable antioxidant capacity of the fruit.
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Affiliation(s)
- Monde A Nyila
- Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, University of South Africa Science Campus, Roodepoort, Johannesburg, South Africa
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Aly SH, Uba AI, Nilofar N, Majrashi TA, El Hassab MA, Eldehna WM, Zengin G, Eldahshan OA. Chemical composition and biological activity of lemongrass volatile oil and n-Hexane extract: GC/MS analysis, in vitro and molecular modelling studies. PLoS One 2025; 20:e0319147. [PMID: 39999113 PMCID: PMC11856542 DOI: 10.1371/journal.pone.0319147] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/19/2024] [Accepted: 01/28/2025] [Indexed: 02/27/2025] Open
Abstract
Lemon grass, formally identified as Cymbopogon citratus, is a plant that belongs to the Poaceae family. The present work aimed to examine the chemical composition by GC/MS analysis and assess the biological potential of C. citratus volatile oil and n-hexane extract. The volatile oil and n-hexane extract were evaluated for antioxidant potential and tested for their enzyme inhibition against tyrosinase, butyrylcholinesterase (BChE), acetylcholinesterase (AChE), α-amylase, and α-glucosidase. The chemical analysis of the lemongrass n-hexane extract (HE) and volatile oil (VO) revealed that the main constituents in the HE are aliphatic hydrocarbons (42.98%), triterpenoids (20.14%), and aromatic hydrocarbons (17.25%). Conversely, the main constituents of the (VO) are predominantly monoterpenes, namely α-citral (36.08%), β-citral (34.22%), and β-myrcene (13.84%). The oil showed more potent antioxidant potential in DPPH, ABTS, CUPRAC, FRAP, and phosphomolybdenum (10.18, 35.69 mg Trolox equivalent/g, 98.97 and 69.73 mg Trolox equivalent/g and 43.01 mmol Trolox equivalent/g). The HE displayed higher BChE (1.53 mg Galanthamine equivalent)/g), as well as α-amylase and α-glucosidase inhibitory activities (0.39 and 2.40 mmol Acarbose equivalent/g). The VO demonstrated more potent tyrosinase inhibitory activities (57.19 mg Kojic acid equivalent/g) along with acetyl and butyrylcholinesterase inhibition. Dominant compounds exhibited the ability to bind with high affinity to various target proteins, with a particular affinity for AChE and BChE. The volatile oil and n-hexane extract of C. citratus show significant promise as a viable choice for the advancement of novel therapeutic strategies aimed at addressing oxidative stress, neurodegeneration, and diabetes.
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Affiliation(s)
- Shaza H. Aly
- Department of Pharmacognosy, Faculty of Pharmacy, Badr University in Cairo (BUC), Cairo, Egypt,
| | - Abdullahi Ibrahim Uba
- Department of Molecular Biology and Genetics, Istanbul AREL University, Istanbul, Turkey,
| | - Nilofar Nilofar
- Department of Biology, Science Faculty, Selcuk University, Konya, Turkey,
- Department of Pharmacy, Botanic Garden “Giardino dei Semplici”, Università degli Studi “Gabriele d’Annunzio”, via dei Vestini 31, Chieti, Italy,
| | - Taghreed A. Majrashi
- Department of Pharmacognosy, College of Pharmacy, King Khalid University, Asir, Saudi Arabia,
| | - Mahmoud A. El Hassab
- Department of Medicinal Chemistry, Faculty of Pharmacy, King Salman International University (KSIU), South Sinai, Egypt,
| | - Wagdy M. Eldehna
- Department of Pharmaceutical of Chemistry, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh, Egypt,
- Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Pharos University in Alexandria, Canal El Mahmoudia St., Alexandria, Egypt,
| | - Gokhan Zengin
- Department of Biology, Science Faculty, Selcuk University, Konya, Turkey,
| | - Omayma A. Eldahshan
- Pharmacognosy Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
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Iommelli P, Spina AA, Vastolo A, Infascelli L, Lotito D, Musco N, Tudisco R. Functional and Economic Role of Some Mediterranean Medicinal Plants in Dairy Ruminants' Feeding: A Review of the Effects of Garlic, Oregano, and Rosemary. Animals (Basel) 2025; 15:657. [PMID: 40075939 PMCID: PMC11898197 DOI: 10.3390/ani15050657] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/16/2025] [Revised: 02/21/2025] [Accepted: 02/22/2025] [Indexed: 03/14/2025] Open
Abstract
Botanical and herbal supplements contain diverse compounds that support health and prevent disease by influencing biological pathways. In ruminant nutrition, such supplements are rarely used as most integrations focus on production improvements using synthetic additives. This review explores the potential of Mediterranean herbs, namely, garlic, oregano, and rosemary, in dairy ruminant diets, emphasizing their effects on milk production, antioxidant activity, weaning management, in vitro studies, and economic impacts. Using medicinal plants reduces reliance on synthetic additives, lowers costs, enhances animal health, and boosts productivity. These plants align with sustainable farming practices, enable premium market access, and support local resources to improve performance and reduce livestock's environmental footprint.
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Affiliation(s)
- Piera Iommelli
- Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Napoli, Italy; (P.I.); (D.L.); (N.M.); (R.T.)
| | - Anna Antonella Spina
- Department of Health Science, University “Magna Graecia” of Catanzaro, 88100 Catanzaro, Italy;
| | - Alessandro Vastolo
- Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Napoli, Italy; (P.I.); (D.L.); (N.M.); (R.T.)
| | - Lorenzo Infascelli
- Department of Economics and Law, University of Cassino, 03043 Cassino, Italy;
| | - Daria Lotito
- Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Napoli, Italy; (P.I.); (D.L.); (N.M.); (R.T.)
| | - Nadia Musco
- Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Napoli, Italy; (P.I.); (D.L.); (N.M.); (R.T.)
| | - Raffaella Tudisco
- Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Napoli, Italy; (P.I.); (D.L.); (N.M.); (R.T.)
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Dahmani W, Akissi ZLE, Elaouni N, Bouanani NE, Mekhfi H, Bnouham M, Legssyer A, Sahpaz S, Ziyyat A. Carob leaves: Phytochemistry, antioxidant properties, vasorelaxant effect and mechanism of action. JOURNAL OF ETHNOPHARMACOLOGY 2025; 340:119226. [PMID: 39653104 DOI: 10.1016/j.jep.2024.119226] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/07/2024] [Revised: 12/04/2024] [Accepted: 12/06/2024] [Indexed: 12/13/2024]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Ceratonia siliqua L., is a species of significant nutritional and industrial interest with extensive traditional uses. This fabaceae is renowned for its medicinal properties, including the treatment of high blood pressure. Due to its chemical composition, carob exhibits several valuable therapeutic functions such as antioxidant, antidiarrheal, antidiabetic, and antibacterial actions. AIM OF THE REVIEW This study investigates the chemical composition of Ceratonia siliqua L. leaves aqueous extract (CsAE) and explores the vasorelaxant effect and its underlying mechanisms. Acute toxicity and antioxidant activity of CsAE were also examined. METHODS The phytochemical profile was elucidated using TLC and UHPLC-MS. The vasorelaxant effect and mechanisms were studied on thoracic aortic rings from normotensive rats, using various antagonists. Acute toxicity was assessed by orally administering the extract to mice. Antioxidant activity was evaluated using β-carotene bleaching and DPPH. RESULTS TLC analysis of CsAE reveals flavonoids and hydrolysable tannins. Gallic acid, myricitrin, quercitrin as well as galloylglucopyranoside derivatives were identified by UHPLC-MS. CsAE relaxed phenylephrine-precontracted aorta in a concentration-dependent manner. This response was reduced when the aorta was denuded or pretreated with L-NAME, hydroxocobalamin, ODQ, 4-AP, TEA, calmidazolium chloride, and thapsigargin. CsAE showed significant antioxidant activity with no observed toxicity in the experimental animals. CONCLUSION CsAE has a significant vasodilatory effect, mediated through the CaM/eNOS/sGC pathway, activation of Kca and Kv, and intracellular calcium mobilization into SERCA. It also exhibits strong antioxidant activity, with no observed toxicity in the experimental animals. These findings represent the first evidence of the vasorelaxant effect of Ceratonia siliqua L. leaves from Eastern Morocco.
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Affiliation(s)
- Widad Dahmani
- Laboratory of Bioresources, Biotechnologies, Ethnopharmacology and Health, Department of Biology, Faculty of Sciences, University Mohammed First, Oujda, 60000, Morocco.
| | - Zachée Louis Evariste Akissi
- BioEcoAgro Joint Cross-Border Research Unit 1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d'Opale, 59650, Villeneuve d'Ascq, France.
| | - Nabia Elaouni
- Laboratory of Bioresources, Biotechnologies, Ethnopharmacology and Health, Department of Biology, Faculty of Sciences, University Mohammed First, Oujda, 60000, Morocco.
| | - Nour Elhouda Bouanani
- Laboratory of Bioresources, Biotechnologies, Ethnopharmacology and Health, Department of Biology, Faculty of Sciences, University Mohammed First, Oujda, 60000, Morocco.
| | - Hassane Mekhfi
- Laboratory of Bioresources, Biotechnologies, Ethnopharmacology and Health, Department of Biology, Faculty of Sciences, University Mohammed First, Oujda, 60000, Morocco.
| | - Mohamed Bnouham
- Laboratory of Bioresources, Biotechnologies, Ethnopharmacology and Health, Department of Biology, Faculty of Sciences, University Mohammed First, Oujda, 60000, Morocco.
| | - Abdelkhaleq Legssyer
- Laboratory of Bioresources, Biotechnologies, Ethnopharmacology and Health, Department of Biology, Faculty of Sciences, University Mohammed First, Oujda, 60000, Morocco.
| | - Sevser Sahpaz
- BioEcoAgro Joint Cross-Border Research Unit 1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d'Opale, 59650, Villeneuve d'Ascq, France.
| | - Abderrahim Ziyyat
- Laboratory of Bioresources, Biotechnologies, Ethnopharmacology and Health, Department of Biology, Faculty of Sciences, University Mohammed First, Oujda, 60000, Morocco.
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Patti M, Musarella CM, Spampinato G. Ethnobotanical knowledge in Calabria (southern Italy): A summary review. Heliyon 2025; 11:e42050. [PMID: 39911427 PMCID: PMC11795066 DOI: 10.1016/j.heliyon.2025.e42050] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/15/2024] [Revised: 01/15/2025] [Accepted: 01/15/2025] [Indexed: 02/07/2025] Open
Abstract
This paper presents a comprehensive overview of ethnobotanical knowledge in Calabria, southern Italy. The diverse plant uses and knowledge in the region stem from the cultural contributions of various populations that have inhabited the area since ancient times. To achieve the stated objective, an in-depth review of 16 bibliographic sources published between 1950 and 2024 was conducted, and data on ethnobotanical uses was extracted. The data were then analyzed using various indices, including CI, CV, FC, PPV, RFC, RI, and UV, to determine the most relevant species, families, and plant parts. A total of 4873 records were collected. The analysis shows that the Asteraceae family is the most used, while Urtica dioica L. and Sambucus nigra L. are the most frequently used species according to the CV index. Medicinal and alimentary purposes are the most common types of use. This study could serve as a foundation for further detailed research, contributing to the valorisation of the ethnobotanical heritage of this region.
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Affiliation(s)
- Miriam Patti
- AGRARIA Department, Mediterranea University of Reggio Calabria, 89122, Reggio Calabria, Italy
| | - Carmelo Maria Musarella
- AGRARIA Department, Mediterranea University of Reggio Calabria, 89122, Reggio Calabria, Italy
| | - Giovanni Spampinato
- AGRARIA Department, Mediterranea University of Reggio Calabria, 89122, Reggio Calabria, Italy
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Aflaha R, Dzaki MR, Katriani L, As'ari AH, Maharani CN, Kuncaka A, Natsir TA, Rianjanu A, Gupta R, Triyana K, Roto R. A polyaniline-enhanced quartz crystal microbalance sensor for room-temperature camphor detection. ANALYTICAL METHODS : ADVANCING METHODS AND APPLICATIONS 2025; 17:724-734. [PMID: 39693141 DOI: 10.1039/d4ay01859a] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/19/2024]
Abstract
A method to detect camphor gas is considered indispensable in the pharmaceutical industry. Unfortunately, the available sensors to detect the presence of camphor in the air are very limited and still on a laboratory scale, such as using chromatography-mass spectroscopy (GC-MS). The research's main focus is to obtain a portable sensing system with excellent sensitivity and selectivity. This study explored polyaniline (PANi) concentrations cast over PVAc nanofiber as a matrix to detect camphor gas using a quartz crystal microbalance (QCM) system to measure camphor exposure. Scanning electron microscopy (SEM) and Fourier-transform infrared (FTIR) were used to analyze the morphology and chemical composition of the fabricated active layer (i.e., nanofiber with PANi thin film). Increasing the PANi concentration provides more PANi on the sensor surface, thus amassing the active groups to interact with camphor gas molecules. It shows that a sensor with a 0.08% PANi thin film (Nano-PANi8) has a sensitivity of 2.594 Hz ppm-1, much greater than the sensor without PANi, which is only 0.305 Hz ppm-1. In addition, the sensor also has good repeatability and rapid response and recovery time of 47 s and 133 s, respectively. Compared to other gaseous compounds, the sensor also has excellent selectivity for camphor and robust long-term stability over three weeks of testing. The produced QCM sensor employing PANi thin film can give a camphor sensor superior performance, including excellent sensitivity, selectivity, and long-term stability. Furthermore, the use of QCM as a base sensor also makes the fabricated sensor portable.
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Affiliation(s)
- Rizky Aflaha
- Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, BLS 21, Yogyakarta 55281, Indonesia
| | - Muammar Romiz Dzaki
- Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, BLS 21, Yogyakarta 55281, Indonesia.
| | - Laila Katriani
- Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, BLS 21, Yogyakarta 55281, Indonesia
- Department of Physics Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Yogyakarta 55281, Indonesia
| | - Ahmad Hasan As'ari
- Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, BLS 21, Yogyakarta 55281, Indonesia
- Research Center for Nanotechnology Systems, National Research and Innovation Agency, Building 440-442, KST B. J. Habibie, Tangerang Selatan 15314, Indonesia
| | - Chlara Naren Maharani
- Department of Physics Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Yogyakarta 55281, Indonesia
| | - Agus Kuncaka
- Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, BLS 21, Yogyakarta 55281, Indonesia.
| | - Taufik Abdillah Natsir
- Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, BLS 21, Yogyakarta 55281, Indonesia.
| | - Aditya Rianjanu
- Department of Materials Engineering, Institut Teknologi Sumatera, Terusan Ryacudu, Way Hui, Jati Agung, Lampung 35365, Indonesia
- Center for Green and Sustainable Materials, Institut Teknologi Sumatera, Terusan Ryacudu, Way Hui, Jati Agung, Lampung 35365, Indonesia
| | - Ruchi Gupta
- School of Chemistry, University of Birmingham, Birmingham, B15 2TT, UK
| | - Kuwat Triyana
- Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, BLS 21, Yogyakarta 55281, Indonesia
| | - Roto Roto
- Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, BLS 21, Yogyakarta 55281, Indonesia.
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Kahlon MS, Moidul Islam M, Vashishat A, Raikwar S. Revolutionizing Rheumatoid Arthritis Care: AI-infused Herbal Treatments and the Road Ahead. Curr Pharm Biotechnol 2025; 26:316-318. [PMID: 38757330 DOI: 10.2174/0113892010305528240506114408] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/19/2024] [Revised: 04/08/2024] [Accepted: 04/22/2024] [Indexed: 05/18/2024]
Affiliation(s)
- Milan Singh Kahlon
- Department of Pharmaceutics, ISF College of Pharmacy, GT Road, Moga, 142001, Punjab, India
| | - Md Moidul Islam
- Department of Pharmaceutics, ISF College of Pharmacy, GT Road, Moga, 142001, Punjab, India
| | - Abhinav Vashishat
- Department of Pharmaceutics, ISF College of Pharmacy, GT Road, Moga, 142001, Punjab, India
| | - Sarjana Raikwar
- Department of Pharmaceutics, ISF College of Pharmacy, GT Road, Moga, 142001, Punjab, India
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25
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Singh S, Kumar S, Singh AK, Varshney M, Roy S. Exploring Marine Alkaloids: A Natural Approach to Cancer Treatment. Curr Pharm Biotechnol 2025; 26:63-79. [PMID: 38918975 DOI: 10.2174/0113892010316791240611093022] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/04/2024] [Revised: 05/15/2024] [Accepted: 05/23/2024] [Indexed: 06/27/2024]
Abstract
Cancer is one of the most complicated and prevalent diseases in the world, and its incidence is growing worldwide. Natural products containing pharmacological activity are widely used in the pharmaceutical industry, especially in anticancer drugs, due to their diverse structures and distinctive functional groups that inspire new drug results by means of synthetic chemistry. Terrestrial medicinal plants have traditionally been the primary source for developing natural products (NPs). However, over the past thirty years, marine organisms such as invertebrates, plants, algae, and bacteria have revealed many new pharmaceutical compounds known as marine NPs. This field constantly evolves as a discipline in molecular targeted drug discovery, incorporating advanced screening tools that have revolutionised and become integral to modern antitumor research. This review discusses recent studies on new natural anticancer alkaloids obtained from marine organisms. The paper illustrates the structure and origin of marine alkaloids and demonstrates the cytotoxic action of new alkaloids from several structural families and their synthetic analogs. The most recent findings about the potential or development of some of them as novel medications, together with the status of our understanding of their current mechanisms of action, are also compiled.
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Affiliation(s)
- Sonia Singh
- Institute of Pharmaceutical Research, GLA University, 17km Stone, NH-2, Mathura-Delhi Road Mathura, Chaumuhan, Uttar Pradesh, 281406, India
| | - Surendra Kumar
- Institute of Pharmaceutical Research, GLA University, 17km Stone, NH-2, Mathura-Delhi Road Mathura, Chaumuhan, Uttar Pradesh, 281406, India
| | - Amit Kumar Singh
- Institute of Pharmaceutical Research, GLA University, 17km Stone, NH-2, Mathura-Delhi Road Mathura, Chaumuhan, Uttar Pradesh, 281406, India
| | - Mayuri Varshney
- Maya Institute of Pharmacy, Hathras, Uttar Pradesh, 204101, India
| | - Suchismita Roy
- Disto Pharmaceuticals Pvt Ltd Unit 2, 209/A, Phase-3 IDA, Pashamaylaram Patancheru, Sangareddy, district, Hyderabad, Telangana, 502307, India
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An C, Li D, Lu L, Liu C, Xu X, Xie S, Wang J, Liu R, Yang C, Qin Y, Zheng P. Insights into the Genomic Background of Nine Common Chinese Medicinal Plants by Flow Cytometry and Genome Survey. PLANTS (BASEL, SWITZERLAND) 2024; 13:3536. [PMID: 39771235 PMCID: PMC11679336 DOI: 10.3390/plants13243536] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 10/27/2024] [Revised: 12/05/2024] [Accepted: 12/16/2024] [Indexed: 01/11/2025]
Abstract
Medicinal plants have long played a crucial role in healthcare systems, but limited genomic information on these species has impeded the integration of modern biological technologies into medicinal plant research. In this study, we selected nine common medicinal plants, each belonging to a different plant family, including Sarcandra glabra (Chloranthaceae), Nekemias grossedentata (Vitaceae), Uraria crinita (Fabaceae), Gynostemma pentaphyllum (Cucurbitaceae), Reynoutria japonica (Polygonaceae), Pseudostellaria heterophylla (Caryophyllaceae), Morinda officinalis (Rubiaceae), Vitex rotundifolia (Lamiaceae), and Gynura formosana (Asteraceae), to estimate their genome sizes and conduct preliminary genomic surveys. The estimated genome sizes by flow cytometry were 3.66 Gb, 0.65 Gb, 0.58 Gb, 1.02 Gb, 3.96 Gb, 2.99 Gb, 0.43 Gb, 0.78 Gb, and 7.27 Gb, respectively. The genome sizes of M. officinalis, R. japonica, and G. pentaphyllum have been previously reported. Comparative analyses suggest that variations in genome size may arise due to differences in measurement methods and sample sources. Therefore, employing multiple approaches to assess genome size is necessary to provide more reliable information for further genomic research. Based on the genome survey, species with considerable genome size variation or polyploidy, such as G. pentaphyllum, should undergo a ploidy analysis in conjunction with population genomics studies to elucidate the development of the diversified genome size. Additionally, a genome survey of U. crinita, a medicinal plant with a relatively small genome size (509.08 Mb) and of considerable interest in southern China, revealed a low heterozygosity rate (0.382%) and moderate repeat content (51.24%). Given the limited research costs, this species represents a suitable candidate for further genomic studies on Leguminous medicinal plants characteristic of southern China. This foundational genomic information will serve as a critical reference for the sustainable development and utilization of these medicinal plants.
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Affiliation(s)
- Chang An
- Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; (C.A.); (D.L.); (L.L.); (C.L.); (X.X.); (S.X.); (J.W.); (R.L.)
| | - Denglin Li
- Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; (C.A.); (D.L.); (L.L.); (C.L.); (X.X.); (S.X.); (J.W.); (R.L.)
| | - Lin Lu
- Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; (C.A.); (D.L.); (L.L.); (C.L.); (X.X.); (S.X.); (J.W.); (R.L.)
| | - Chaojia Liu
- Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; (C.A.); (D.L.); (L.L.); (C.L.); (X.X.); (S.X.); (J.W.); (R.L.)
| | - Xiaowen Xu
- Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; (C.A.); (D.L.); (L.L.); (C.L.); (X.X.); (S.X.); (J.W.); (R.L.)
| | - Shiyu Xie
- Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; (C.A.); (D.L.); (L.L.); (C.L.); (X.X.); (S.X.); (J.W.); (R.L.)
| | - Jing Wang
- Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; (C.A.); (D.L.); (L.L.); (C.L.); (X.X.); (S.X.); (J.W.); (R.L.)
| | - Ruoyu Liu
- Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; (C.A.); (D.L.); (L.L.); (C.L.); (X.X.); (S.X.); (J.W.); (R.L.)
- Pingtan Science and Technology Research Institute, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
| | - Chengzi Yang
- College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;
| | - Yuan Qin
- Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; (C.A.); (D.L.); (L.L.); (C.L.); (X.X.); (S.X.); (J.W.); (R.L.)
- Pingtan Science and Technology Research Institute, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
| | - Ping Zheng
- Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; (C.A.); (D.L.); (L.L.); (C.L.); (X.X.); (S.X.); (J.W.); (R.L.)
- Pingtan Science and Technology Research Institute, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
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Tanasă F, Nechifor M, Teacă CA. Essential Oils as Alternative Green Broad-Spectrum Biocides. PLANTS (BASEL, SWITZERLAND) 2024; 13:3442. [PMID: 39683235 DOI: 10.3390/plants13233442] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/31/2024] [Revised: 11/25/2024] [Accepted: 12/04/2024] [Indexed: 12/18/2024]
Abstract
Natural compounds from plants represent suitable options to replace synthetic biocides when employed against microorganisms in various applications. Essential oils (EOs) have attracted increased interest due to their biocompatible and rather innocuous nature, and complex biological activity (fungicide, biocide and anti-inflammatory, antioxidant, immunomodulatory action, etc.). EOs are complex mixtures of derived metabolites with high volatility obtained from various vegetal parts and employed to a great extent in different healthcare (natural cures, nutrition, phyto- and aromatherapy, spices) and cosmetics applications (perfumery, personal and beauty care), as well as in cleaning products, agriculture and pest control, food conservation and active packaging, or even for restauration and preservation of cultural artifacts. EOs can act in synergy with other compounds, organic and synthetic as well, when employed in different complex formulations. This review will illustrate the employment of EOs in different applications based on some of the most recent reports in a systematic and comprehensive, though not exhaustive, manner. Some critical assessments will also be included, as well as some perspectives in this regard.
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Affiliation(s)
- Fulga Tanasă
- Polyaddition and Photochemistry Department, "Petru Poni" Institute of Macromolecular Chemistry, 41A Gr. Ghica-Voda Alley, 700487 Iasi, Romania
| | - Marioara Nechifor
- Polyaddition and Photochemistry Department, "Petru Poni" Institute of Macromolecular Chemistry, 41A Gr. Ghica-Voda Alley, 700487 Iasi, Romania
| | - Carmen-Alice Teacă
- Center of Advanced Research in Bionanoconjugates and Biopolymers, "Petru Poni" Institute of Macromolecular Chemistry, 41A Gr. Ghica-Voda Alley, 700487 Iasi, Romania
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28
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Choi SM, Choi CI. Nutritional Composition, Phytochemical Profiles, and Pharmacological Effects of Ethiopian Eggplant ( Solanum aethiopicum L.). Nutrients 2024; 16:4228. [PMID: 39683621 DOI: 10.3390/nu16234228] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/01/2024] [Revised: 12/01/2024] [Accepted: 12/04/2024] [Indexed: 12/18/2024] Open
Abstract
Natural product therapy has been used to treat illness for thousands of years, and modern-day medicines, such as various anticancer, antihypertensive, and antimigraine drugs, have been developed from natural products. Natural medicines are advantageous as they tend to have fewer side effects and are considered a relatively safe option. Solanum aethiopicum L. (S. aethiopicum) is a vegetable crop of the Solanaceae family and is considered one of the five most important crops in sub-Saharan Africa, alongside tomatoes, onions, peppers, and okra. S. aethiopicum has many health benefits as it contains the three major macronutrients (carbohydrates, proteins, and fats) as well as fiber and many essential vitamins. Additionally, much research has been conducted on the medicinal value of S. aethiopicum over the past few decades. S. aethiopicum has been found to have many pharmacological properties including anti-inflammatory, antibacterial, antidiabetic, anti-obesity, and antioxidant effects. Currently, to our knowledge, there are no comprehensive reviews of the numerous studies on S. aethiopicum. Therefore, in this review, we summarize the nutritional, phytochemical, and pharmacological analyses of S. aethiopicum, identify notable effects, and review the results.
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Affiliation(s)
- Seung Min Choi
- Integrated Research Institute for Drug Development, College of Pharmacy, Dongguk University-Seoul, Goyang 10326, Republic of Korea
| | - Chang-Ik Choi
- Integrated Research Institute for Drug Development, College of Pharmacy, Dongguk University-Seoul, Goyang 10326, Republic of Korea
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Negasa JG, Teshome I, Sarba EJ, Daro BS. Phytochemical screening and in vitro antibacterial activity of Echinops kebericho Mesfin tuber extracts: experimental studies. PeerJ 2024; 12:e18554. [PMID: 39650562 PMCID: PMC11623108 DOI: 10.7717/peerj.18554] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/24/2024] [Accepted: 10/29/2024] [Indexed: 12/11/2024] Open
Abstract
Background The application of plant extracts and their phytochemicals as potential treatments for bacterial illnesses has increased significantly in the last few decades. In Ethiopia, Echinops kebericho Mesfin is widely used to treat a range of illnesses in humans and animals. This study aimed to evaluate the antibacterial activity and phytochemical screening of Echinops kebericho Mesfin. Methods We carried out an in vitro experimental study after collecting the plants from their natural habitats. Then macerated in absolute methanol and petroleum ether solvents and concentrated the extracts using a rotary evaporator. In the experiment, we used Standard cultures of E. coli, K. pneumoniae, S. aureus, and P. aeruginosa. The agar-well diffusion method evaluated the antibacterial activity of the plants. The agar dilution method determined the minimum inhibitory concentration of the plant extract. Results The percentage yield of the plant extracts ranged from 6.25% to 7.85%. The methanol extract of Echinops kebericho Mesfin had the highest inhibitory effect on S. aureus (ATCC 25923) (16.67 ± 0.58 mm), followed by E. coli (ATCC 25922) (11.0 ± 1.73 mm). Phytochemical screening of leaves from the methanol and petroleum ether extracts of the plant revealed the presence of phytochemicals such as alkaloids, flavonoids, tannins, and cardiac glycosides. The present study revealed that the extracts of these plants have antibacterial activity. However, researchers should conduct further studies on the safety margin and quantitative bioactive isolation of selected medicinal plants.
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Affiliation(s)
- Jiregna Gari Negasa
- Department of Veterinary Laboratory Technology, School of Veterinary Medicine, Ambo University, Ambo, Oromia, Ethiopia
| | - Ibsa Teshome
- School of Veterinary Medicine, Ambo University, Ambo, Oromia, Ethiopia
| | - Edilu Jorga Sarba
- Department of Veterinary Science, Ambo University, Ambo, Oromia, Ethiopia
| | - Bekiyad Shasho Daro
- Department of Veterinary Epidemiology, College of Veterinary Medicine, Haramaya University, Dire dawa, Oromia, Ethiopia
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30
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Kota S, Nelapati AK, Govada VR. Plant resources for immunonutrients and immunomodulators to combat infectious respiratory viral diseases: a review. 3 Biotech 2024; 14:302. [PMID: 39554986 PMCID: PMC11568085 DOI: 10.1007/s13205-024-04143-y] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/05/2024] [Accepted: 10/26/2024] [Indexed: 11/19/2024] Open
Abstract
Boosting the immune system has become a crucial aspect in the global battle against the COVID-19 pandemic and other similar infections to protect oneself against symptoms, especially in the prevention of viral infections of the lower respiratory tract. The importance of conducting more studies to create successful herbal formulations as infection prevention measures is emphasized in this review, which looks at the function of immune-boosting nutrients, medicinal plants, and herbal treatments. We reviewed and analyzed 207 studies published from 1946 to the present using reputable databases like Google Scholar, PubMed, and NCBI. The review examined 115 plant species in total and identified 12 key nutrients, including vitamins A, D, C, omega-3 fatty acids, iron, and zinc, while noting that four plant families, Rosaceae, Asteraceae, Amaryllidaceae, and Acanthaceae, show potential against respiratory infections like influenza, RSV, and SARS-CoV. To lower the risk of infection, it is recommended to consume nutritious meals that have immune-modulating qualities. Information on the bioactive components of medicinal herbs, spices, and plants that have been effective in treating respiratory viral infections and related conditions is compiled in this review, which highlights phytoactive substances with antibacterial and antiviral activity as effective modulators to lower the risk of infections. Furthermore, it is highlighted that ancient knowledge systems, like Ayurveda and Naturopathy, should be integrated to help develop new herbal formulations. To improve immunity and lessen vulnerability to serious respiratory infections, the results highlight the need for including immune-modulating foods and plant-based medicines into everyday routines.
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Affiliation(s)
- Sobha Kota
- Department of Chemical Engineering, RVR & JC College of Engineering, Guntur, Andhra Pradesh 522 019 India
| | - Anand Kumar Nelapati
- Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research, Vadlamudi, Guntur, Andhra Pradesh 522 213 India
| | - Vayunandana Rao Govada
- Department of Chemical Engineering, RVR & JC College of Engineering, Guntur, Andhra Pradesh 522 019 India
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Naser EH, Idries AH, Elmubarak SAA, Dafalla MB, Abdelrahim YE, Abdalrhman EA, Ahmed BM, Osman MEM, Awadallah AKE, Ebrahim RMA, Abdellatif AO, Saad HA, Konozy EHE. Isolation, purification, and characterization of lectins from medicinal plant Combretum glutinosum seeds endowed with analgesic and antiulcer properties. Biochimie 2024; 227:273-285. [PMID: 39102999 DOI: 10.1016/j.biochi.2024.08.003] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/19/2024] [Revised: 06/19/2024] [Accepted: 08/02/2024] [Indexed: 08/07/2024]
Abstract
In the pursuit of safer and more effective treatments, there is a growing interest in plant-derived compounds, particularly lectins, because of their diverse pharmacological properties. This study focused on isolating, purifying, and characterizing lectin from Combretum glutinosum seeds (CGSLs) to assess its potential as an analgesic and antiulcer agent. CGSL extraction involved defatting and buffer extraction, followed by purification using ammonium sulfate fractionation and fetuin-agarose affinity column chromatography. The isolectins (iso-CGSLs), each consisting of 60 kDa and 57 kDa heterodimeric subunits, displayed glycoprotein properties with a 40 % neutral sugar content. They exhibited peak activity at 55 °C and remained stable for up to the fifth day at room temperature. The activity exhibited a pH dependence, peaking between 7.5 and 10.5, and all seemingly operated independently of metal ions. CGSL, at optimal doses ranging from 6 to 12 mg/kg, had significant analgesic effects on acetic acid-induced writhing and hot plate tests in mice. Evaluation using 0.7 % acetic acid resulted in notable pain reduction across all doses (P < 0.05). The analgesic effect of lectin was partially reversed by naloxone (a morphine antagonist), indicating partial involvement of the opioid receptor system. Furthermore, CGSL exhibited antiulcer effects in ethanol-induced gastric ulcer models in rats, highlighting its therapeutic potential as a natural alternative for analgesic and antiulcer treatments.
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Affiliation(s)
- Eva H Naser
- Biotechnology Department, Africa City of Technology, Khartoum, Sudan
| | - Ahmed H Idries
- Biotechnology Department, Africa City of Technology, Khartoum, Sudan
| | | | - Maha B Dafalla
- Biotechnology Department, Africa City of Technology, Khartoum, Sudan
| | | | | | - Bashir M Ahmed
- Medicinal, Aromatic Plants and Traditional Medicine Research Institute (MAPTRI), National Center for Research, Khartoum, Sudan
| | | | | | - Reem M A Ebrahim
- Biotechnology Department, Africa City of Technology, Khartoum, Sudan
| | - Ashraf O Abdellatif
- Pharmaceutical Research and Development Centre, Faculty of Pharmacy, Karary University, Omdurman, Sudan
| | - Haseeba A Saad
- Department of Zoology, Faculty of Science, University of Khartoum, Khartoum, Sudan
| | - Emadeldin H E Konozy
- Biotechnology Department, Africa City of Technology, Khartoum, Sudan; Pharmaceutical Research and Development Centre, Faculty of Pharmacy, Karary University, Omdurman, Sudan; Biomedical and Clinical Research Centre (BCRC), College of Health and Allied Sciences (CoHAS), University of Cape Coast, Cape Coast, Ghana.
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Abbas Z, Mustafa S, Khan MF, Khan MA, Massey S, Dev K, Khan A, Parveen S, Husain SA. Therapeutic importance of Kigelia africana subsp. africana: an alternative medicine. Nat Prod Res 2024; 38:4208-4222. [PMID: 37921076 DOI: 10.1080/14786419.2023.2273914] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/24/2023] [Revised: 09/22/2023] [Accepted: 10/15/2023] [Indexed: 11/04/2023]
Abstract
AIM To summarise a detailed up-to-date review of the traditional uses, phytoconstituents, and pharmacological activities of various parts of Kigelia africana. MATERIALS AND METHODS Google Scholar, PubMed, PubChem, Elsevier, King Draw, indianbiodiversity.org. RESULT The phytochemical analysis of Kigelia africana subsp. africana has revealed the presence of approximately 145 compounds extracted from different parts of the plant. These bioactive extracts of the plant possess anti-inflammatory, antioxidant, antimicrobial, antidiabetic, antineoplastic, and anti-urolithic activities. Due to its anti-inflammatory, antioxidant, and immune-booster properties, Kigelia can prove to be an essential source of drugs for treating various disorders. CONCLUSION Knowledge of the phytoconstituents, non-medicinal and medicinal traditional uses, pharmacological activities, and products obtained from Kigelia is described in this review with the hope that the updated findings will promote research on its biological pathways.
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Affiliation(s)
- Zahra Abbas
- Department of Biosciences, Human Genetics Laboratory, Jamia Millia Islamia, New Delhi, India
| | - Saad Mustafa
- Department of Biosciences, Human Genetics Laboratory, Jamia Millia Islamia, New Delhi, India
- Deen Dayal Upadhyay Kaushal Kendra, Jamia Millia Islamia, New Delhi, India
| | - Mohd Faisal Khan
- Department of Biotechnology, Medical Biotechnology Laboratory, Jamia Millia Islamia, New Delhi, India
| | - Mohammad Aasif Khan
- Department of Biosciences, Human Genetics Laboratory, Jamia Millia Islamia, New Delhi, India
| | - Sheersh Massey
- Department of Biosciences, Human Genetics Laboratory, Jamia Millia Islamia, New Delhi, India
| | - Kapil Dev
- Department of Biotechnology, Medical Biotechnology Laboratory, Jamia Millia Islamia, New Delhi, India
| | - Asifa Khan
- Department of Biosciences, Human Genetics Laboratory, Jamia Millia Islamia, New Delhi, India
| | - Shabana Parveen
- Department of Biosciences, Human Genetics Laboratory, Jamia Millia Islamia, New Delhi, India
| | - Syed Akhtar Husain
- Department of Biosciences, Human Genetics Laboratory, Jamia Millia Islamia, New Delhi, India
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Hu D, Hu L, Xiao O, Chen J, Dai X, Sun Y, Kong Z. Bibliometric analysis of research trends and advancements in medicinal plant microbiome. FRONTIERS IN PLANT SCIENCE 2024; 15:1495198. [PMID: 39634064 PMCID: PMC11614653 DOI: 10.3389/fpls.2024.1495198] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 09/12/2024] [Accepted: 11/04/2024] [Indexed: 12/07/2024]
Abstract
Medicinal plants and microorganisms are closely linked, with microorganisms boosting plant growth, offering pest control, and enhancing secondary compound production. However, there's a lack of systematic research, detailed molecular studies, and standardized methods for effectively using microorganisms in developing products from medicinal plants. To enhance understanding of the present research progress, emerging patterns, and key areas pertaining to microorganisms found in medicinal plants, CiteSpace bibliometric software was employed to visualize and analyze 1269 English publications sourced from the Science Net Core Collection database. Through the utilization of keyword co-occurrence analysis and cluster analysis methods, this study seeks to explore collaborative networks among countries, institutions, and scholars involved in the study of microorganisms in medicinal plants. This review highlights key research areas in microbiology, focusing on evaluating natural compounds for antibacterial properties and the impact of secondary metabolites on microbial communities, aiming to highlight significant research domains and primary focuses for researchers and professionals engaged in the field of microbiology concerning medicinal plants.
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Affiliation(s)
- Danling Hu
- State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
- Key Laboratory of Forage and Endemic Crop Biotechnology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, China
| | - Lizhe Hu
- State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
- Key Laboratory of Forage and Endemic Crop Biotechnology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, China
| | - Ouli Xiao
- State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
| | - Jieyin Chen
- State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
- Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji, China
| | - Xiaofeng Dai
- State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
| | - Yongwei Sun
- Key Laboratory of Forage and Endemic Crop Biotechnology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, China
| | - Zhiqiang Kong
- State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
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Onder A. Recent progress on Prangos (Apiaceae) species used in traditional herbal medicine. JOURNAL OF ETHNOPHARMACOLOGY 2024; 334:118480. [PMID: 38909827 DOI: 10.1016/j.jep.2024.118480] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/19/2024] [Revised: 05/21/2024] [Accepted: 06/20/2024] [Indexed: 06/25/2024]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Plants have been used for a long time in traditional medicine to treat many diseases. The genus Prangos belongs to the Apiaceae family and has various medicinal and aromatic species. Since ancient times, Prangos species have been employed extensively in traditional medicine for different purposes and are especially popular for their aphrodisiac effects. AIM OF THE REVIEW The goal of this paper is to represent a systematic review of the species in the genus Prangos, including their botanical characteristics, uses in traditional medicine, phytochemical constituents, the composition of the essential oils produced, and the biological properties. MATERIALS AND METHODS The articles regarding traditional uses and bioactivities of Prangos species were evaluated using electronic databases such as PubMed, Google Scholar, and ScienceDirect. Use of the World Flora Online (WFO) - The Plant List, The International Plant Names Index, the World Checklist of Vascular Plants (2024), and ChemDraw Professional helped complete this compilation. RESULTS Phytochemical investigations have indicated that coumarins are characteristic constituents of Prangos species, especially prenylated simple coumarins and furanocoumarins, and also flavonoids, terpenoids, and phytosterols occur in this genus. In addition, the essential oils of these plants have been examined. The biological properties of the Prangos species seem worthy of further investigation. Also, some information about the toxicity of these species and their use as ingredients in food products is presented. CONCLUSIONS This review highlights the evaluation of traditional knowledge, phytochemical profiles, biological activities, and potential uses of Prangos species as foods and spices. Many pharmacological activities have been performed related to their traditional uses, but frequently, the exact mechanism of action remains scientifically unproven. This review has compiled data on the phytochemistry, the active secondary metabolites, the biological properties, and recent advances in Prangos species.
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Affiliation(s)
- Alev Onder
- Ankara University, Faculty of Pharmacy, Department of Pharmacognosy, 06100, Tandogan-Ankara, Türkiye.
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Peiró-Vila P, Luján-Roca I, Baeza-Baeza JJ, Torres-Lapasió JR, García-Alvarez-Coque MC. Transferability of global retention models in reversed-phase liquid chromatography for natural products. J Chromatogr A 2024; 1736:465410. [PMID: 39378623 DOI: 10.1016/j.chroma.2024.465410] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/24/2024] [Revised: 09/17/2024] [Accepted: 09/29/2024] [Indexed: 10/10/2024]
Abstract
Considerable progress has been made in enhancing resolution in reversed-phase liquid chromatography for the analysis of complex samples, particularly within the field of natural products, through the application of global retention models using multi-linear gradients. Global models effectively differentiate solute retention effects from those originating from the column and solvent, offering predictive capabilities comparable to conventional individual retention models, without the requirement for standards for all compounds. While conventional individual models result in higher accuracy, they frequently demand standards that are unavailable for natural product samples. Moreover, the creation of individual models can be time-consuming due to the need for repetitive work for additional compounds. Experimental validation of global models has demonstrated that the accuracy is enough for the prediction of complex chromatograms. Through a carefully designed experimental work, this study reports the correct determination of global parameters for column and solvent, with excellent consistency across various medicinal plant samples. The successful transfer of predictions and optimisation of resolution across diverse plant species (lemon balm, peppermint, and pennyroyal) is confirmed. This highlights the applicability of predictions using global models across botanical varieties.
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Affiliation(s)
- P Peiró-Vila
- Department of Analytical Chemistry, Faculty of Chemistry, Universitat de València, C/ Dr. Moliner 50, 46100 Burjassot, Spain
| | - I Luján-Roca
- Department of Analytical Chemistry, Faculty of Chemistry, Universitat de València, C/ Dr. Moliner 50, 46100 Burjassot, Spain
| | - J J Baeza-Baeza
- Department of Analytical Chemistry, Faculty of Chemistry, Universitat de València, C/ Dr. Moliner 50, 46100 Burjassot, Spain
| | - J R Torres-Lapasió
- Department of Analytical Chemistry, Faculty of Chemistry, Universitat de València, C/ Dr. Moliner 50, 46100 Burjassot, Spain.
| | - M C García-Alvarez-Coque
- Department of Analytical Chemistry, Faculty of Chemistry, Universitat de València, C/ Dr. Moliner 50, 46100 Burjassot, Spain
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Mkhonto C, Mokgehle SN, Mbeng WO, Ramarumo LJ, Ndlhovu PT. Review of Mimusops zeyheri Sond. (Milkwood): Distribution, Utilisation, Ecology and Population Genetics. PLANTS (BASEL, SWITZERLAND) 2024; 13:2943. [PMID: 39458890 PMCID: PMC11511078 DOI: 10.3390/plants13202943] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/22/2024] [Revised: 10/03/2024] [Accepted: 10/09/2024] [Indexed: 10/28/2024]
Abstract
Mimusops zeyheri Sond. (Milkwood) is an indigenous fruit tree species with considerable ecological, cultural, and nutritional significance that remains underexploited. This review synthesizes current knowledge on its distribution, taxonomy, phytochemistry, ethnomedicinal applications, ecological functions, genetic diversity, and biotechnological potential. A systematic literature search, spanning 1949 to April 2024, yielded 87 relevant publications from an initial 155. Mimusops zeyheri plays a crucial role in supporting the cultural traditions and economic activities of Indigenous Southern African Communities. Its distribution encompasses South, East, and Southern Tropical Africa, with substantial populations across South African provinces. Ethnomedicinally, various plant parts treat conditions including wounds, gastrointestinal issues, and diabetes. The leaves (34%) and roots (32%) are used, with infusion (33%) and decoction (31%) as primary preparation methods. Oral administration (70%) is the most common, primarily addressing skin conditions (18%). Despite its nutritional richness, a standardized nutrient profile is lacking. Limited genetic diversity studies underscore the need for further research. This study highlights Mimusops zeyheri's multifaceted importance and research gaps, particularly in other Southern African countries. Future investigations should focus on comprehensive phytochemical analysis, ethnomedicinal validation, ecological conservation, genetic diversity assessment, and biotechnological applications. Multidisciplinary collaborations are recommended to promote sustainable utilization while preserving traditional practices.
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Affiliation(s)
- Christeldah Mkhonto
- School of Biology and Environmental Sciences, Faculty of Agriculture and Natural Sciences, University of Mpumalanga, Private Bag X11283, Mbombela 1200, South Africa
| | - Salmina Ngoakoana Mokgehle
- School of Agricultural Sciences, Faculty of Agriculture and Natural Sciences, University of Mpumalanga, Private Bag X11283, Mbombela 1200, South Africa
| | - Wilfred Otang Mbeng
- School of Biology and Environmental Sciences, Faculty of Agriculture and Natural Sciences, University of Mpumalanga, Private Bag X11283, Mbombela 1200, South Africa
| | - Luambo Jeffrey Ramarumo
- School of Biology and Environmental Sciences, Faculty of Agriculture and Natural Sciences, University of Mpumalanga, Private Bag X11283, Mbombela 1200, South Africa
| | - Peter Tshepiso Ndlhovu
- School of Biology and Environmental Sciences, Faculty of Agriculture and Natural Sciences, University of Mpumalanga, Private Bag X11283, Mbombela 1200, South Africa
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Espíndola-Rodríguez NH, Muñoz-Cázares N, Serralta-Peraza LEDS, Díaz-Nuñez JL, Montoya-Reyes F, García-Contreras R, Díaz-Guerrero M, Rivera-Chávez JA, Gutiérrez J, Sotelo-Barrera M, Castillo-Juárez I. Antivirulence and antipathogenic activity of Mayan herbal remedies against Pseudomonas aeruginosa. JOURNAL OF ETHNOPHARMACOLOGY 2024; 332:118373. [PMID: 38782309 DOI: 10.1016/j.jep.2024.118373] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/22/2024] [Revised: 05/11/2024] [Accepted: 05/19/2024] [Indexed: 05/25/2024]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE The Yucatan Peninsula has a privileged wealth of vascular plants with which various Mayan herbal formulations have been developed. However, studies on their antipathogenic and antivirulence properties are scarce. AIM OF THE STUDY Identify antivirulence properties in Mayan herbal remedies and determine their antipathogenic capacity in burn wounds infected with Pseudomonas aeruginosa. MATERIALS AND METHODS An ethnobotanical study was conducted in Mayan communities in central and southern Quintana Roo, Mexico. Furthermore, the antipathogenic capacity of three Mayan herbal remedies was analyzed using an animal model of thermal damage and P. aeruginosa infection. Antivirulence properties were determined by inhibiting phenotypes regulated by quorum sensing (pyocyanin, biofilm, and swarming) and by the secretion of the ExoU toxin. The chemical composition of the most active herbal remedy was analyzed using molecular network analysis. RESULTS It was found that topical administration of the remedy called "herbal soap" (HS) for eleven days maintained 100% survival of the animals, reduced establishment of the bacteria in the burn and prevented its systemic dispersion. Although no curative effect was recorded on tissue damaged by HS treatment, its herbal composition strongly reduced swarming and ExoU secretion. Through analysis of Molecular Networks, it was possible to carry out a global study of its chemical components, and identify the family of oxindole monoterpenoid alkaloids and carboline and tetrahydropyrididole alkaloids. In addition, flavonols, flavan-3-ols, and quinic acid derivatives were detected. CONCLUSIONS The antipathogenic and antivirulence capacity of ancient Mayan remedies makes them a potential resource for developing new antibacterial therapies to treat burns infected by P. aeruginosa.
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Affiliation(s)
- Nadine Heidi Espíndola-Rodríguez
- Laboratorio de Investigación y Aplicación de Fitoquímicos Bioactivos, Posgrado en Botánica, Colegio de Postgraduados, Texcoco, 56230, Mexico.
| | - Naybi Muñoz-Cázares
- Campo Experimental Chetumal, Instituto de Investigaciones Forestales, Agrícolas y Pecuarias, Quintana Roo, 77963, Mexico; Investigadora Posdoctoral CONAHCYT Comisionada al Colegio de Postgraduados-Campus Tabasco, Cárdenas, Tabasco, C. P. 86500, Mexico.
| | | | - José Luis Díaz-Nuñez
- Laboratorio de Investigación y Aplicación de Fitoquímicos Bioactivos, Posgrado en Botánica, Colegio de Postgraduados, Texcoco, 56230, Mexico.
| | - Francisco Montoya-Reyes
- Campo Experimental Chetumal, Instituto de Investigaciones Forestales, Agrícolas y Pecuarias, Quintana Roo, 77963, Mexico.
| | - Rodolfo García-Contreras
- Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, CDMX, 04510, Mexico.
| | - Miguel Díaz-Guerrero
- Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, CDMX, 04510, Mexico.
| | - José Alberto Rivera-Chávez
- Departamento de Productos Naturales, Instituto de Química, Universidad Nacional Autónoma de México, CDMX, 04510, Mexico.
| | - Jorge Gutiérrez
- Área de Biología, Departamento de Preparatoria Agrícola, Universidad Autónoma Chapingo, Texcoco, 56230, Mexico.
| | - Mireya Sotelo-Barrera
- Laboratorio de Investigación y Aplicación de Fitoquímicos Bioactivos, Posgrado en Botánica, Colegio de Postgraduados, Texcoco, 56230, Mexico.
| | - Israel Castillo-Juárez
- Laboratorio de Investigación y Aplicación de Fitoquímicos Bioactivos, Posgrado en Botánica, Colegio de Postgraduados, Texcoco, 56230, Mexico; Conahcyt-Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Mineral de la Reforma, Hidalgo, 42184, Mexico.
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Sharma V, Sinha ES, Singh J. Investigation of In Vitro Anti-cancer and Apoptotic Potential of Onion-Derived Nanovesicles Against Prostate and Cervical Cancer Cell Lines. Appl Biochem Biotechnol 2024; 196:6957-6973. [PMID: 38441782 DOI: 10.1007/s12010-024-04872-z] [Citation(s) in RCA: 1] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 02/12/2024] [Indexed: 11/21/2024]
Abstract
Plant-derived compounds have recently garnered significant interest in the field of medicine due to their rich repertoire of phytochemicals, which holds promise for exploring novel therapies to treat cancer. This study embarks on the first-time investigation of the anti-cancerous effect of onion-derived nanovesicles (ODNVs). ODNVs were isolated employing differential centrifugation followed by ultracentrifugation and subsequent characterization using dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM), and Fourier transform infrared spectroscopy (FTIR). Furthermore, we delineated the anti-cancerous effect of ODNVs on two cancer cell line models HeLa (cervical cancer) and PC-3 (prostate cancer) using MTT assay, DAPI-based DNA damage using immunofluorescence microscopy, colony formation assay, migration assay, cell cycle analysis, and evaluation of apoptosis using flow cytometry and western blotting. The findings revealed dose- and time-dependent anti-proliferative effects of ODNVs on both HeLa and PC3 cell lines, accompanied by selective cytotoxicity against cancer cells. Additional results highlighted that ODNVs prevented colony growth and induced S-phase cell cycle arrest. Apoptosis induction was evaluated through alterations in nuclear morphology and the number of apoptotic cells, which increased significantly after ODNV treatment in both cancer cell lines. Furthermore, annexin V/PI staining evaluation of apoptotic cells by flow cytometry demonstrated that ODNV treatment significantly increased the number of apoptotic cells in both PC-3 and HeLa cells. Finally, Western blot analysis indicated changes in apoptosis-related proteins including bcl-2, bax, and caspase-3, emphasizing that the anti-cancerous effect of ODNVs is attributed to the induction of apoptosis and suggests the unexplored anti-cancerous potential of ODNVs.
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Affiliation(s)
- Vinayak Sharma
- Department of Biotechnology, Panjab University, Chandigarh, 160014, India
| | - Eshu Singhal Sinha
- Department of Biotechnology, Panjab University, Chandigarh, 160014, India
| | - Jagtar Singh
- Department of Biotechnology, Panjab University, Chandigarh, 160014, India.
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Parveen M, Uzma, Khan AA, Nami SAA, Kataria R, Malik A, Amali NAM, Abd Kadir NH, Alam M. Isolation, Characterization, and Single-Crystal X-ray Analysis of Lantabetulic Acid from Rhus alata: Insights into HSA and BSA Binding Interactions, with In-Silico Study. ACS OMEGA 2024; 9:39484-39502. [PMID: 39346876 PMCID: PMC11425619 DOI: 10.1021/acsomega.4c03406] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 04/09/2024] [Revised: 09/01/2024] [Accepted: 09/04/2024] [Indexed: 10/01/2024]
Abstract
This study investigated the bioactive potential of Rhus alata, a plant known for its rich phytochemicals. A previously unreported compound was isolated from R. alata and characterized using various spectroscopic techniques (IR, UV, NMR, MS) and confirmed for the first time by X-ray crystallography. In isolated compound 1, noncovalent interactions between H···H/H···H, C···C/C···C and O···H/H···O play a major role in its packing arrangement. This observation is consistent with the results of Hirshfeld surface analysis, which quantified these interactions as 14.2%, 84.6%, and 1.2%, respectively. The isolated compound was identified as lantabetulic acid (1) (3β,25-expoxy-3α-hydroxylup-20(29)-en-28-oic acid). To understand its potential biological interactions, the binding affinity of lantabetulic acid to biomolecules such as bovine serum albumin (BSA), and human serum albumin (HSA), was assessed. The results showed significant binding efficacy, indicating potential interactions with these molecules. Furthermore, the DPPH assay demonstrated the potent antioxidant activity of this compound. We used in silico molecular docking to clarify the binding affinity between lantabetulic acid and a particular receptor. Furthermore, molecular dynamic simulation studies also explored the binding interaction. As well, MM/GBSA calculations corroborate the simulation results and the stability of the complex. Docking and dynamics studies revealed promising binding scores, suggesting further investigation into their potential therapeutic applications. Geometric parameters and the absorption spectrum of compound 1 were also determined using the DFT approach and compared with experimental findings.
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Affiliation(s)
- Mehtab Parveen
- Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India
| | - Uzma
- Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India
| | - Azmat Ali Khan
- Pharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
| | - Shahab A A Nami
- Department of Industrial Chemistry, Aligarh Muslim University, Aligarh 202002, India
| | - Ramesh Kataria
- Department of Chemistry, Panjab University, Chandigarh 160014, India
| | - Abdul Malik
- Department of Pharmaceutics. College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
| | - Nurul Afiqah Muhammad Amali
- Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia
| | - Nurul Huda Abd Kadir
- Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia
| | - Mahboob Alam
- Department of Safety Engineering, Dongguk University, 123 Dongdae-ro, Gyeongju, Gyeongbuk 780714, Republic of Korea
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40
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Rai P, Garain T, Gupta D. Phytochemical analysis of Tinospora cordifolia and Withania somnifera and their therapeutic activities with special reference to COVID-19. World J Exp Med 2024; 14:95512. [PMID: 39312696 PMCID: PMC11372741 DOI: 10.5493/wjem.v14.i3.95512] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 04/11/2024] [Revised: 07/19/2024] [Accepted: 07/29/2024] [Indexed: 08/29/2024] Open
Abstract
Various important medicines make use of secondary metabolites that are produced by plants. Medicinal plants, such as Withania somnifera and Tinospora cordifolia, are rich sources of chemically active compounds and are reported to have numerous therapeutic applications. The therapeutic use of medicinal plants is widely mentioned in Ayurveda and has folkloric importance in different parts of the world. The aim of this review is to summarize the phytochemical profiles, folkloric importance, and primary pharmacological activity of W. somnifera and T. cordifolia with emphasis on their action against the novel coronavirus.
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Affiliation(s)
- Prateek Rai
- Chemistry, Amity Institute of Applied Sciences, Amity University, Noida 201301, Uttar Pradesh, India
| | - Tanya Garain
- Chemistry, Amity Institute of Applied Sciences, Amity University, Noida 201301, Uttar Pradesh, India
| | - Deepshikha Gupta
- Chemistry, Amity Institute of Applied Sciences, Amity University, Noida 201301, Uttar Pradesh, India
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Nuro G, Tolossa K, Arage M, Giday M. Medicinal plants diversity among the oromo community in heban-arsi district of Ethiopia used to manage human and livestock ailments. Front Pharmacol 2024; 15:1455126. [PMID: 39372211 PMCID: PMC11450779 DOI: 10.3389/fphar.2024.1455126] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/26/2024] [Accepted: 08/28/2024] [Indexed: 10/08/2024] Open
Abstract
Introduction Medicinal plants are commonly employed mainly due their accessibility, affordability and potency. However, medicinal plants and the associated knowledge are disappearing at an alarming rate due to natural and anthropogenic causes and thus a need for their proper documentation conservation. This study was performed to document traditional knowledge related to use of medicinal plants in Heban-Arsi district, West-Arsi Zone, Oromia Regional State, Ethiopia. Methods Interviews were conducted with 185 informants to identify medicinal plants used in traditional therapies in the study area. Informant consensus factor (ICF), rank order priority (ROP) values were computed, and preference ranking exercises were performed to assess the relative importance of medicinal plants. Descriptive and inferential statistics were used to measure and compare medicinal plants knowledge between social groups. Results A total of 120 medicinal plants were identified for being used to treat different human and animal illnesses in the study area. Most of the medicinal plants (76.4%) were uncultivated ones obtained from different habitats. Leaf was the most frequently used plant part constituting 62.6% of preparations. Oral was the most commonly used route of remedy administration (46%) in treating diseases. Gastrointestinal ailments category had the highest ICF value (0.83). In the study area, the highest rank order priority (ROP) values were recorded for Dombeya torrida (J.F. Gmel.), Artemisia absinthium L., Balanites aegyptiaca (L.) Del., Combretum pisoniiflorum Klotzsch) Engl., Celtis africana Burm. f, Ocimum gratissimum L. and Lagenaria sp. for their uses against snake poison, tuberculosis, liver disorder, stomachache, tuberculosis, febrile illness and liver disorder, respectively, each scoring a value of 100. Significant differences in medicinal plant use knowledge were recorded between male and female informants of different educational level, age and experience. Anthropogenic factors were the primary threats to medicinal plants in the area. Conclusion The study area was found to be rich in medicinal plants that are useful in treating a wide range of human and animal illnesses. In future pharmacological and phytochemical investigations, priority needs to be given to medicinal plants of the highest ROP values and those that were reported against ailment categories scoring the highest ICF values.
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Affiliation(s)
| | | | | | - Mirutse Giday
- Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia
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Adu-Amankwaah F, Februarie C, Nyambo K, Maarman G, Tshililo N, Mabasa L, Mavumengwana V, Baatjies L. Cytotoxic properties, glycolytic effects and high-resolution respirometry mitochondrial activities of Eriocephalus racemosus against MDA-MB 231 triple-negative breast cancer. BMC Complement Med Ther 2024; 24:332. [PMID: 39256791 PMCID: PMC11389270 DOI: 10.1186/s12906-024-04615-x] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/17/2024] [Accepted: 08/14/2024] [Indexed: 09/12/2024] Open
Abstract
INTRODUCTION Triple-negative breast cancer (TNBC) represents a significant global health crisis due to its resistance to conventional therapies and lack of specific molecular targets. This study explored the potential of Eriocephalus racemosus (E. racemosus) as an alternative treatment for TNBC. The cytotoxic properties and high-resolution respirometry mitochondrial activities of E. racemosus against the MDA-MB 231 TNBC cell line were evaluated. METHODS Hexane solvent and bioactive fraction extractions of E. racemosus were performed, while mass spectrometry-based metabolite profiling was used to identify the phytochemical constituents of the extracts. The extracts were further tested against MDA-MB 231 TNBC cells to determine their cytotoxicity. The mode of cell death was determined using flow cytometry. The activities of caspases 3, 8, and 9 were assessed using a multiplex activity assay kit. Glycolytic activity and High-resolution respirometry measurements of mitochondrial function in the MDA-MB 231 cell line were conducted using the Seahorse XFp and Oroboros O2K. RESULTS Metabolite profiling of E. racemosus plant crude extracts identified the presence of coumarins, flavonoids, sesquiterpenoids, triterpenoids, and unknown compounds. The extracts demonstrated promising cytotoxic activities, with a half maximal inhibitory concentration (IC50) of 12.84 µg/mL for the crude hexane extract and 15.49 µg/mL for the bioactive fraction. Further, the crude hexane and bioactive fraction extracts induced apoptosis in the MDA-MB-231 TNBC cells, like the reference drug cisplatin (17.44%, 17.26% and 20.25%, respectively) compared to untreated cells. Caspase 3 activities confirmed the induction of apoptosis in both cisplatin and the plant crude extracts, while caspase 8 and 9 activities confirmed the activation of both the intrinsic and extrinsic apoptosis pathways. Increased levels of glycolytic activity were observed in the hexane crude extract. High-resolution respiratory measurements showed elevated mitochondrial activities in all mitochondrial states except for complex-IV activity. CONCLUSION These findings support further exploration of E. racemosus as a potential therapeutic agent for TNBC, offering a promising avenue for the development of targeted treatments with minimal adverse effects.
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Affiliation(s)
- Francis Adu-Amankwaah
- South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa
| | - Candice Februarie
- Division of Medical Physiology, Department of Biomedical Sciences, Faculty of Medicine & Health Science, CARMA: Centre for Cardio-Metabolic Research in Africa, Stellenbosch University, Cape Town, 8000, South Africa
| | - Kudakwashe Nyambo
- South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa
| | - Gerald Maarman
- Division of Medical Physiology, Department of Biomedical Sciences, Faculty of Medicine & Health Science, CARMA: Centre for Cardio-Metabolic Research in Africa, Stellenbosch University, Cape Town, 8000, South Africa
| | - Ndivhuwo Tshililo
- South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa
| | - Lawrence Mabasa
- Biomedical Research and Innovation Platform (BRIP), Medical Research Council, Tygerberg, Cape Town, South Africa
| | - Vuyo Mavumengwana
- South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa
- Biomedical Research and Innovation Platform (BRIP), Medical Research Council, Tygerberg, Cape Town, South Africa
| | - Lucinda Baatjies
- South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.
- Biomedical Research and Innovation Platform (BRIP), Medical Research Council, Tygerberg, Cape Town, South Africa.
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Raza W, Meena A, Luqman S. THF induces apoptosis by downregulating initiation, promotion, and progression phase biomarkers in skin and lung carcinoma. J Biochem Mol Toxicol 2024; 38:e23838. [PMID: 39243196 DOI: 10.1002/jbt.23838] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/06/2024] [Revised: 07/18/2024] [Accepted: 08/23/2024] [Indexed: 09/09/2024]
Abstract
3,5,7-Trihydroxy-2-phenylchromen-4-one (THF) possesses a diverse range of pharmacological activities. Evidence suggests that THF exerts anticancer activity by distinct mechanisms of action. This study explores the anticancer potential of THF in human lung (A549) and skin (A431) cancer cells by employing different antiproliferative assays. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, neutral red uptake, sulphorhodamine B, and cell motility assays were used to confirm the anticancer potential of THF. Cell target-based and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays were used to explore the effect of THF on the initiation, promotion and progression phase biomarkers of carcinogenesis. THF suppresses the activity of lipoxygenase-5 up to ~40% in both A549 and A431 cells and up to ~50% hyaluronidase activity in A549 cells. qRT-PCR assay reveals that THF inhibits the activity of phosphatidyl inositol-3 kinase/protein kinase B/mammalian target of rapamycin in both cell lines, which is responsible for the initiation of cancer. It also arrests the G2/M phase of the cell cycle in A431 cells and increases the sub-diploid population in both A549 and A431 cell lines which leads to cell death. Annexin V-FITC assay confirmed that THF induces apoptosis and necrosis in A431 and A549 cell lines. Further investigation revealed that THF not only enhances reactive oxygen species production but also modulates mitochondrial membrane potential in both cell lines. It significantly inhibits S-180 tumour formation at 5 and 10 mg/kg bw, i.p. dose. An acute skin toxicity study on mice showed that erythema and edema scores are within the acceptable range, besides acceptable drug-likeness properties and non-toxic effects on human erythrocytes. Conclusively, THF showed potent anticancer activity on skin and lung carcinoma cell lines, suppressed the level of the biomarkers and inhibited tumour growth in mice.
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Affiliation(s)
- Waseem Raza
- Bioprospection and Product Development Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India
- Jawaharlal Nehru University, New Delhi, India
| | - Abha Meena
- Bioprospection and Product Development Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India
- Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India
| | - Suaib Luqman
- Bioprospection and Product Development Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India
- Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India
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Dos Santos ÉRQ, Pantoja LVPDS, Farias SV, Pinheiro BG, Andrade EHA, Mendes PFS, Cruz JN, Monteiro MC, Davis K, Lima RR, Freitas JJDS, Burbano RMR, Prediger RD, Fontes-Junior EA, Maia JGS, Maia CDSF. Linalool-rich rosewood essential oil (Aniba rosaeodora Ducke) mitigates emotional and neurochemical impairments induced by ethanol binge-like exposure during adolescence in female rats. Biomed Pharmacother 2024; 178:117120. [PMID: 39024836 DOI: 10.1016/j.biopha.2024.117120] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/16/2024] [Revised: 07/04/2024] [Accepted: 07/08/2024] [Indexed: 07/20/2024] Open
Abstract
Linalool-rich Rosewood oil (Aniba rosaeodora Ducke) is a natural compound widely used in perfumery industry. Evidence suggests that linalool exerts antidepressant and anxiolytic effects. Conversely, ethanol binge drinking (i.e., intermittent and episodic consumption) during adolescence elicits neurobehavioral alterations associated with brain damage. Here, we investigated whether linalool-rich Rosewood oil administration can improve the emotional and molecular impairments associated with ethanol binge-like exposure during adolescence in female rats. Rosewood oil was obtained by hydrodistillation and posteriorly analyzed. Adolescent female Wistar rats received four-cycles of ethanol binge-like pattern (3 g/kg/day, 3 days on/4 days off) and daily Rosewood oil (35 mg/kg, intranasally) for 28 days. Twenty-four hours after treatments, it was evaluated the impact of ethanol exposure and Rosewood oil treatment on the putative emotional impairments assessed on the splash and forced swimming tests, as well as the levels of brain-derived neurotrophic factor (BDNF), S100B, oxidative parameters, and inflammatory cytokines in prefrontal cortex and hippocampus. Results indicated that Rosewood oil intranasal administration mitigated emotional impairments induced by ethanol exposure accompanied by a marked increase in BDNF, S100B, glutathione (GSH), and antioxidant activity equivalent to Trolox (TEAC) levels in brain areas. Rosewood oil treatment also prevented the ethanol-induced increase of interleukin-1β, interleukin-6, tumor necrosis factor α (TNF-α), and neurofilament light chain (NFL) levels. These findings provide the first evidence that Rosewood oil intranasal administration exerts protective effects against emotional and molecular impairments associated with adolescent ethanol binge-like exposure, possibly due to linalool actions triggering neurotrophic factors, rebalancing antioxidant status, and attenuating proinflammatory process.
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Affiliation(s)
- Éverton Renan Quaresma Dos Santos
- Laboratório de Farmacologia da Inflamação e do Comportamento, Faculdade de Farmácia, Universidade Federal do Pará, Belém, PA, Brazil
| | | | - Sarah Viana Farias
- Laboratório de Farmacologia da Inflamação e do Comportamento, Faculdade de Farmácia, Universidade Federal do Pará, Belém, PA, Brazil
| | - Bruno Gonçalves Pinheiro
- Laboratório de Farmacologia da Inflamação e do Comportamento, Faculdade de Farmácia, Universidade Federal do Pará, Belém, PA, Brazil
| | - Eloisa Helena A Andrade
- Programa de Pós-Graduação em Química, Universidade Federal do Pará, Belém, PA 66075-900, Brazil
| | | | - Jorddy Neves Cruz
- Laboratório de Biologia Funcional e Estrutural, Universidade Federal do Pará, Belém, PA 66075-900, Brazil
| | - Marta Chagas Monteiro
- Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pará, Belém, PA 66075-900, Brazil
| | - Kelly Davis
- Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pará, Belém, PA 66075-900, Brazil
| | - Rafael Rodrigues Lima
- Laboratório de Biologia Funcional e Estrutural, Universidade Federal do Pará, Belém, PA 66075-900, Brazil
| | | | | | - Rui Daniel Prediger
- Departamento de Farmacologia, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina 88049-900, Brazil
| | - Enéas Andrade Fontes-Junior
- Laboratório de Farmacologia da Inflamação e do Comportamento, Faculdade de Farmácia, Universidade Federal do Pará, Belém, PA, Brazil
| | - José Guilherme S Maia
- Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pará, Belém, PA 66075-900, Brazil
| | - Cristiane do Socorro Ferraz Maia
- Laboratório de Farmacologia da Inflamação e do Comportamento, Faculdade de Farmácia, Universidade Federal do Pará, Belém, PA, Brazil.
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Meñiza JF, Pasco MM, Alimbon JA. A review of ethnobotanical studies reveals over 500 medicinal plants in Mindanao, Philippines. PLANT DIVERSITY 2024; 46:551-564. [PMID: 39290882 PMCID: PMC11403148 DOI: 10.1016/j.pld.2024.05.001] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 12/02/2023] [Revised: 05/06/2024] [Accepted: 05/08/2024] [Indexed: 09/19/2024]
Abstract
Medicinal plants have long been used to treat various diseases in both indigenous and non-indigenous populations of Mindanao, Philippines. Here, we extracted data from ethnobotanical studies to compile a comprehensive list of these medicinal plants and identify how and for what purpose they are most commonly used. We identified 530 verified medicinal plant species across 372 genera in 118 families. The two most frequently cited species were Euphorbia hirta and Psidium guajava. The most represented family was Fabaceae and the most represented genus was Ficus. A total of 28 medicinal plant species are designated as threatened at the national or global level; of these, 11 are endemic to the Philippines. Medicinal plant preparations most commonly use leaves for oral administration to treat various diseases such as digestive issues, including diarrhea. This study underscores the need for further ethnobotanical investigations, particularly in areas lacking records. It also emphasizes the need for conservation of threatened and endemic medicinal plants to ensure sustainable utilization of this valuable resource.
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Affiliation(s)
- Joeime F Meñiza
- Department of Teacher Education, UM Tagum College, Tagum City, 8100, Davao del Norte Philippines
| | - Monica M Pasco
- Department of Teacher Education, UM Tagum College, Tagum City, 8100, Davao del Norte Philippines
| | - Jemer A Alimbon
- Department of Teacher Education, UM Tagum College, Tagum City, 8100, Davao del Norte Philippines
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Khadim S, Malik K, Kazmi A, Sultana T, Ali A, Mehmood K, Hassan RU, Bashir MN, Ali MM. Folklore use of medicinal plants for the treatment of gynecological diseases in Pakistan-a review. Heliyon 2024; 10:e34869. [PMID: 39144941 PMCID: PMC11320484 DOI: 10.1016/j.heliyon.2024.e34869] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/16/2024] [Revised: 06/25/2024] [Accepted: 07/17/2024] [Indexed: 08/16/2024] Open
Abstract
Background Gynecological issues and sexually transmitted infections (STIs) pose significant challenges to women's health, particularly in developing nations. These challenges are exacerbated by limited access to modern reproductive healthcare facilities, economic constraints, and entrenched cultural norms. Consequently, most of the Pakistani population relies on traditional ethno-medicinal healthcare systems. This preference stems from the ease of access, affordability, widespread availability, and inherent trust placed in these alternative healthcare methods. Aim/objective The inquiry aimed to report details on the application of conventional uses of plants in the health field in rural areas that could contribute to advancing the natural discovery of drugs. The objective of this analysis is to provide researchers with information on conventional and empirical knowledge of plant species concerning women's diseases. Methodology Information on the common use of medicinal plants in treating women's diseases was gathered from electronic databases. As a keyword for the quest, ethnobotany, and ethnopharmacology were used together with gynecological complications. Result The work of the current analysis has revealed that 217 plant species belonging to 89 families have been used in Pakistan's rural communities. The majority of plant species belong to the Apiaceae family, followed by the Asteraceae, Fabaceae, Solanaceae, Rosaceae, Lamiaceae, and other families. The biological interpretation of plants used in rural communities of Pakistan revealed that herbs and trees are the dominant forms with 58 % and 23 % respectively while shrubs and sub-shrubs with a low percentage of 17 % and 2 %. In natural preparation, leaves 29 %, flowers 22 %, seeds 14 %, fruits 14 %, roots 13 %, bark 7 %, and stems 5 % were the most used parts respectively and aerial parts, dried pericarp, bulb, bud, berry, latex, wood, rhizome, husk, fruit coat, oil, resins, twigs, and shoot were also used in minimum percentage. A multitude of plant species have found extensive application in the management of diverse women's health issues. These encompass concerns such as fatigue, mood fluctuations attributed to menstrual problems, gonorrhea, complications related to pregnancy, cravings for specific foods, throbbing breast pain, abdominal and pelvic cramps, excessive vaginal discharge, mastitis, irritability, abortion-related matters, headaches, uterine hemorrhage, Menorrhagia, Amenorrhea, Menopause, Vomiting Abortion, infertility and lactation challenges, as well as the regulation of lochia flow. Conclusion This review provides remarkable information about the use of medicinal plants against women's diseases in the rural communities of Pakistan. It opens the gateway for the discovery of natural drug development.
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Affiliation(s)
- Salihah Khadim
- Department of Botany, PMAS, Arid Agriculture University, Rawalpindi, Pakistan
| | - Khafsa Malik
- Department of Botany, PMAS, Arid Agriculture University, Rawalpindi, Pakistan
| | - Abeer Kazmi
- The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China
- University of Chinese Academy of Sciences, Beijing, 100049, China
| | - Tahira Sultana
- Department of Botany, PMAS, Arid Agriculture University, Rawalpindi, Pakistan
| | - Amir Ali
- Department of Botany, PMAS, Arid Agriculture University, Rawalpindi, Pakistan
| | - Khalid Mehmood
- Department of Biology, PMAS, Arid Agriculture University, Rawalpindi, Pakistan
| | - Rizwan Ul Hassan
- Department of Chemical & Biological Engineering, Gachon University (13120) 1342 Seongnamdaero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea
| | - Muhammad Nasir Bashir
- Department of Mechanical Engineering, Yonsei University, Seoul, 120-749, Republic of Korea
| | - M. Mahmood Ali
- Department of Mechatronic Engineering, Atlantic Technological University Sligo, Ash Lane, F91 YW50 Sligo, Ireland
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Nerlekar N, Patil P, Khot S, Kulkarni A, Dandge P, Berde A, Kamane S, Ghatage P, Dandge P. Cold maceration extraction of wild fruit Terminalia bellirica (Gaertn.) Roxb.: exploring its bioactives for biomedical applications. Prep Biochem Biotechnol 2024; 54:982-1000. [PMID: 38349742 DOI: 10.1080/10826068.2024.2313632] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 04/05/2024]
Abstract
Terminalia bellirica (T. bellirica) (Gaertn.) Roxb. is a well-known traditional medicinal plants that show promising treatment because of fewer side effects in humans. In the present study, the total phenol, flavonoid, condensed and hydrolyzable tannins extracted and analyzed from cold macerated (CM) T. bellirica (Gaertn.) Roxb. fruit (TBF) and leaves (TBL) extract with the identification of bioactive compounds using GC-MS/MS technique. The highest amount of bioactive content was found in ethanolic extract than toluene. Current experimental data of TBF extract shows the maximum and significant biological activity like free radical scavenging activity against DPPH and FRAP assays with IC50 values of 51.07 ± 0.52 μg/ml and 63.14 ± 0.59 μg/ml respectively. However, IC50 cytotoxicity values of TBF extract on MCF-7 cells for 24 hrs was found to be 6.34 ± 0.72 μg/ml. Minimum inhibitory concentration (MIC) for infectious pathogens Escherichia coli and Bacillus cereus was >12.5 μg/ml and >100 μg/ml respectively, however, anti-inflammatory activity was demonstrated as an IC50 value of 509.1 ± 1.72 μg/ml. Cold macerated fruit extract revealed threatening inhibitory potential against the α-amylase and α-glucosidase enzymes, with IC50 of 50.98 ± 0.23 μg/ml and 46.70 ± 1.38 μg/ml respectively. Finally, the outcome of this study showed that T. bellirica (Gaertn.) Roxb. fruit extract could be an effective source of bioactives with efficient biomedical properties.
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Affiliation(s)
- Nisha Nerlekar
- Department of Biochemistry, Shivaji University, Kolhapur, India
| | - Pradnya Patil
- Department of Chemistry, Shivaji University, Kolhapur, India
| | - Suraj Khot
- Department of Chemistry, Shivaji University, Kolhapur, India
| | - Arati Kulkarni
- Department of Biochemistry, Shivaji University, Kolhapur, India
| | - Prafull Dandge
- Department of Chemistry, Shivaji University, Kolhapur, India
| | - Ajinkya Berde
- Department of Botany, Shivaji University, Kolhapur, India
| | - Shubham Kamane
- School of Earth Sciences, SRTM University, Nanded, India
| | | | - Padma Dandge
- Department of Biochemistry, Shivaji University, Kolhapur, India
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Kozyra M, Biernasiuk A, Gryta E, Kozyra P, Malm A. Phytochemical Profiling and Biological Activity of the Methanolic Extracts of Cirsium Monspessulanum (L.) Hill. Chem Biodivers 2024; 21:e202400944. [PMID: 38828873 DOI: 10.1002/cbdv.202400944] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/16/2024] [Revised: 05/20/2024] [Accepted: 06/03/2024] [Indexed: 06/05/2024]
Abstract
The study of new plant species and the identification of their chemical composition may contribute to the discovery of a new breakthrough substances for pharmacotherapeutical applications. For the first time, we examined antioxidant and antimicrobial activity of 70 % v/v methanolic extracts from inflorescences and roots of Cirsium monspessulanum (L.) Hill. obtained by the ASE method. In the (2,2-diphenyl-1-picrylhydrazyl) DPPH analysis, tested extract of inflorescences showed antioxidant activity with an EC50=0.223±0.0479 mg/mL, and (Cupric Ion Reducting Antioxidant Capacity) CUPRAC test assessed the antiradical activity on 14.95±0.13 mgTE/g and for roots the values were EC50=0.307±0.0554 mg/mL and 11.18±0.49 mgTE/g, respectively. Furthermore, extract from the inflorescences possessed the highest antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermidis and Micrococcus luteus with MIC=1.25 mg/mL for each. HPLC/ESI-QTOF-MS/MS method identified 7 phenolic acids and 14 flavonoids in inflorescences extract and only 7 phenolic acids in roots extract. To the best of our knowledge, this is the first qualitative analysis of Cirsium monspessulanum (L.) Hill. and all substances were described for the first time.
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Affiliation(s)
- Małgorzata Kozyra
- Department of Pharmacognosy with the Medicinal Plant Garden, Medical University of Lublin, PL-20093, Lublin, Poland
| | - Anna Biernasiuk
- Department of Pharmaceutical Microbiology, Medical University of Lublin, PL-20093, Lublin, Poland
| | - Elżbieta Gryta
- Department of Pharmacognosy with the Medicinal Plant Garden, Medical University of Lublin, PL-20093, Lublin, Poland
| | - Paweł Kozyra
- Independent Radiopharmacy Unit, Faculty of Pharmacy, Medical University of Lublin, PL-20093, Lublin, Poland
| | - Anna Malm
- Department of Pharmaceutical Microbiology, Medical University of Lublin, PL-20093, Lublin, Poland
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Lee EB, Lee K. Coptis rhizome extract influence on Streptococcus pneumoniae through autolysin activation. AMB Express 2024; 14:79. [PMID: 38965154 PMCID: PMC11224187 DOI: 10.1186/s13568-024-01736-x] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/05/2024] [Accepted: 06/25/2024] [Indexed: 07/06/2024] Open
Abstract
This study investigated the antibacterial properties of Coptis rhizome, a plant traditionally used for respiratory infections, against Streptoccus pneumonia (S. pneumoniae), for which there has been minimal empirical evidence of effectiveness. The study particularly examined autolysis, indirectly associated with antibacterial resistance, when using Coptis rhizome for bacterial infections. In our methodology, Coptis rhizome was processed with ethanol and distilled water to produce four different extracts: CRET30, CRET50, CRET70, and CRDW. The antibacterial activity of these extracts were tested through Minimum Inhibitory Concentration (MIC) assays, disk diffusion tests, and time-kill assays, targeting both standard (ATCC 49619) and resistant (ATCC 70067) strains. The study also evaluated the extracts' biofilm inhibition properties and monitored the expression of the lyt gene, integral to autolysis. The results prominently showed that the CRET70 extract demonstrated remarkable antibacterial strength. It achieved an MIC of 0.125 µg/mL against both tested S. pneumoniae strains. The disk diffusion assay recorded inhibition zones of 22.17 mm for ATCC 49619 and 17.20 mm for ATCC 70067. Impressively, CRET70 resulted in a 2-log decrease in bacterial numbers for both strains, showcasing its potent bactericidal capacity. The extract was also effective in inhibiting 77.40% of biofilm formation. Additionally, the significant overexpression of the lytA gene in the presence of CRET70 pointed to a potential mechanism of action for its antibacterial effects. The outcomes provided new perspectives on the use of Coptis rhizome in combating S. pneumoniae, especially significant in an era of escalating antibiotic resistance.
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Affiliation(s)
- Eon-Bee Lee
- Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea
- Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon, 39660, Republic of Korea
| | - Kyubae Lee
- Department of Biomedical Materials, Konyang University, Daejeon, 35365, Republic of Korea.
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50
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Ojo OA, Ajeigbe D, Ogunlakin AD, Odesanmi OE, Ayomipo M, Berana G, Ayeni P, Ajayi-Odoko OA, Ayokunle DI, Ojo AB, Ajiboye BO, Ojo OO, Dahunsi SO. Preclinical antidiabetic and antioxidant effects of Erythrophleum africanum (benth.) harms in streptozotocin-induced diabetic nephropathy. JOURNAL OF COMPLEMENTARY & INTEGRATIVE MEDICINE 2024; 0:jcim-2024-0090. [PMID: 38954410 DOI: 10.1515/jcim-2024-0090] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/26/2024] [Accepted: 06/16/2024] [Indexed: 07/04/2024]
Abstract
OBJECTIVES This study investigated the antidiabetic effects of the methanolic extract of E. africanum (MEEA) stem bark on streptozotocin (STZ)-induced diabetic nephropathy (DN) in Wistar rats. METHODS The in vitro enzyme (α-amylase) inhibitory activity of MEEA was measured using a standard procedure. Diabetic rats with fasting blood glucose above 250 mg/dL were considered diabetic and were divided into the following groups: control (distilled water-treated), diabetic-control, diabetic metformin (100 mg/kg), diabetes + MEEA (150 mg/kg), and diabetes + MEEA (300 mg/kg) via oral gavage once daily for 14 days. At the end of the experimental period, kidney tissues were collected for biochemical and histological analyses. Kidney apoptosis and marker gene expression were measured by real-time quantitative PCR. RESULTS MEEA exhibited α-amylase inhibitory effects. MEEA significantly (p<0.05) reduced the STZ-induced increases in blood glucose, serum urea, serum creatinine, uric acid, alanine aminotransferase, alkaline phosphatase, and malondialdehyde and increased the STZ-induced decreases in superoxide dismutase, catalase, and reduced glutathione. In addition, MEEA protects against DN by significantly downregulating the mRNA expression of cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), cAMP-response binding protein (CREB), and cFOS and upregulating B-cell lymphoma 2 (Bcl-2), suggesting that the nephroprotective ability of MEEA is due to the modulation of the cAMP/PKA/CREB/cFOS signaling pathway. Furthermore, MEEA treatment protected against histopathological alterations observed in diabetic rats. CONCLUSIONS The data from this study suggest that MEEA modulates glucose homeostasis and inhibits redox imbalance in DN rats.
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Affiliation(s)
- Oluwafemi A Ojo
- 70671 Good Health and Wellbeing Research Clusters (SDG 03), Bowen University , Iwo, Nigeria
- Biochemistry Programme, 70671 Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Bowen University , Iwo, Nigeria
| | - David Ajeigbe
- 70671 Good Health and Wellbeing Research Clusters (SDG 03), Bowen University , Iwo, Nigeria
- Biochemistry Programme, 70671 Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Bowen University , Iwo, Nigeria
| | - Akingbolabo D Ogunlakin
- 70671 Good Health and Wellbeing Research Clusters (SDG 03), Bowen University , Iwo, Nigeria
- Biochemistry Programme, 70671 Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Bowen University , Iwo, Nigeria
| | | | - Mojisola Ayomipo
- 70671 Good Health and Wellbeing Research Clusters (SDG 03), Bowen University , Iwo, Nigeria
- Biochemistry Programme, 70671 Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Bowen University , Iwo, Nigeria
| | - Godwin Berana
- 70671 Good Health and Wellbeing Research Clusters (SDG 03), Bowen University , Iwo, Nigeria
- Biochemistry Programme, 70671 Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Bowen University , Iwo, Nigeria
| | - Peluola Ayeni
- 70671 Good Health and Wellbeing Research Clusters (SDG 03), Bowen University , Iwo, Nigeria
- Biochemistry Programme, 70671 Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Bowen University , Iwo, Nigeria
| | | | | | - Adebola B Ojo
- Department of Biochemistry, Ekiti State University, Ado-Ekiti, Nigeria
| | - Basiru O Ajiboye
- Department of Biochemistry, Federal University Oye Ekiti, Oye Ekiti, Nigeria
| | - Omolara O Ojo
- Department of Biochemistry, Ekiti State University, Ado-Ekiti, Nigeria
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