Zhou M, Yoshikawa K, Akashi H, Miura M, Suzuki R, Li TS, Abe H, Bando Y. Localization of ATP-sensitive K+ channel subunits in rat liver. World J Exp Med 2019; 9(2): 14-31 [PMID: 31938690 DOI: 10.5493/wjem.v9.i2.14]
Corresponding Author of This Article
Ming Zhou, MD, PhD, Assistant Professor, Department of Anatomy, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan. mzhou@med.akita-u.ac.jp
Research Domain of This Article
Medicine, Research & Experimental
Article-Type of This Article
Basic Study
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Zhou M, Yoshikawa K, Akashi H, Miura M, Suzuki R, Li TS, Abe H, Bando Y. Localization of ATP-sensitive K+ channel subunits in rat liver. World J Exp Med 2019; 9(2): 14-31 [PMID: 31938690 DOI: 10.5493/wjem.v9.i2.14]
Ming Zhou, Hideo Akashi, Ryoji Suzuki, Yoshio Bando, Department of Anatomy, Akita University Graduate School of Medicine, Akita 010-8543, Japan
Kiwamu Yoshikawa, Mitsutaka Miura, Department of Cell Biology and Morphology, Akita University Graduate School of Medicine, Akita 010-8543, Japan
Tao-Sheng Li, Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan
Hiroshi Abe, TRUST, A Long-Term Care Health Facility, Sendai 980-0011, Japan
Author contributions: Zhou M did the main experiments, collected the data, and wrote the paper; Yoshikawa K and Miura M performed the experiment of primary culture for hepatic stellate cells and Kupffer cells; Akashi H and Suzuki R participated in treatment of animals; Li TS, Abe H, and Bando Y supervised the investigation and approved the manuscript for publication.
Supported bythe Program of the network-type joint Usage/Research Center for Radiation Disaster Medical Science of Hiroshima University, Nagasaki University, and Fukushima Medical University.
Institutional review board statement: This study was approved by the Animal Research Committee of Akita University.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Animal Research Committee of Akita University (a-1-2405).
Conflict-of-interest statement: Authors declare no conflicts of interest for this article.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Corresponding author: Ming Zhou, MD, PhD, Assistant Professor, Department of Anatomy, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan. mzhou@med.akita-u.ac.jp
Telephone: +81-18-8846260 Fax: +81-18-8846440
Received: May 17, 2019 Peer-review started: May 20, 2019 First decision: August 2, 2019 Revised: September 5, 2019 Accepted: November 20, 2019 Article in press: November 20, 2019 Published online: December 19, 2019 Processing time: 215 Days and 5.6 Hours
Core Tip
Core tip: ATP-sensitive K+ (KATP) channels have been found to be ubiquitously distributed in a variety of cell types and tissues, but the present study revealed that five kinds of KATP channel subunits are expressed in rat liver by applying western blot analysis, immunohistochemistry, and immunocytochemistry of rat liver tissue and primary cultured cells. These KATP channel subunits were not only localized in hepatocytes but also in sinusoidal cells, such as hepatic stellate cells, Kupffer cells, and sinusoidal endothelial cells. This was demonstrated using specific marker labelling, such as GFAP for hepatic stellate cells, CD68 for Kupffer cells, and SE-1 for sinusoidal endothelial cells. The wide diversity of KATP channel subunit compositions in rat liver might correspond to their various functions in hepatocytes and sinusoidal cells.
