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Wicaksono S, Ngokwe ZB, McCullough M, Yap T. The Role of Oral Yeasts in the Development and Progression of Oral Squamous Cell Carcinoma: A Scoping Review. J Fungi (Basel) 2025; 11:260. [PMID: 40278081 PMCID: PMC12028735 DOI: 10.3390/jof11040260] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/15/2025] [Revised: 03/25/2025] [Accepted: 03/26/2025] [Indexed: 04/26/2025] Open
Abstract
The role of oral yeasts in oral squamous cell carcinoma (OSCC) has gained attention due to evidence linking fungal dysbiosis to carcinogenesis. While Candida albicans has been the primary focus, emerging studies highlight the importance of non-Candida species yeast genera. This scoping review synthesises the evidence on the role of oral yeasts, including Candida spp. and non-Candida species, in the development and progression of OSCC. A PRISMA-ScR-guided search was conducted in Medline, Embase, EBM Reviews, and CINAHL. Observational and experimental studies involving humans with OSCC, oral potentially malignant disorders (OPMDs), or oral epithelial dysplasia (OED) were included. This review analysed 75 studies. Research on oral yeast in OSCC has progressed since the 1970s, with advancements in identification techniques-from conventional culture methods to metagenomic sequencing and multi-omics approaches-alongside improved animal and cellular models of OSCC. These methodological advancements have identified notable distinctions in the oral mycobiome between carcinomatous and healthy states. Clinical findings reinforce the hypothesis that oral yeasts, particularly Candida spp., actively contribute to the dysplasia-carcinoma sequence. Emerging evidence suggests that oral yeasts may significantly modulate events contributing to OSCC progression. However, further mechanistic studies and robust clinical evidence are essential to establish causality and clarify their role in OSCC.
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Affiliation(s)
| | | | | | - Tami Yap
- Melbourne Dental School, University of Melbourne, Carlton, VIC 3053, Australia; (S.W.); (Z.B.N.); (M.M.)
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Tsai WC, Liu FL, Huang MH, Huang CH. Enhancing Immunity and Modulating Vaginal Microflora Against Candidal Vaginitis Through Nanoemulsion Supplemented with Porphyra Oligosaccharide as an Intravaginal Vaccine Adjuvant. Int J Nanomedicine 2023; 18:6333-6346. [PMID: 37954454 PMCID: PMC10637204 DOI: 10.2147/ijn.s431009] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/17/2023] [Accepted: 10/27/2023] [Indexed: 11/14/2023] Open
Abstract
Background Intravaginal vaccination is an encouraging approach to prevent infectious vaginitis, with nanoemulsions showing effectiveness as mucosal adjuvants. Purpose This study aimed to formulate a nanoemulsion incorporating Porphyra oligosaccharide (PO@NE) and assess its effectiveness as a mucosal adjuvant in intravaginal vaccines against candidal vaginitis. Materials and Methods PO@NE was prepared, and the stability, immunomodulatory activity and cytotoxicity were screened in vitro. Further, the preventive effect of PO@NE as adjuvants for heat-killed Candida albicans (HK-CA) vaccines was explored in a murine model of candidal vaginitis, in comparison with those supplemented with polysaccharide (PP@NE). The mice were intravaginally vaccinated with 106 HK-CA cells, suspended in 1% NE without or with either PO or PP at a final concentration of 6.5 μg/mL, in a total volume of 20 μL. This vaccination was intravaginally administered once a week for 3 weeks. One week following the final vaccination, the mice underwent an intravaginal challenge with 107 C. albicans cells. One week after the challenge, the mice were euthanized to isolate serum, spleen, vaginal washes, and vaginal tissues for analysis. Results PP@NE and PO@NE, with diameters approximately around 100 nm, exhibited exceptional stability at 4°C and low cytotoxicity when used at a concentration of 1% (v/v). Intravaginal vaccination with HK-CA adjuvanted with PO@NE effectively protected against candidal vaginitis evidenced by less Candida hyphae colonization, milder mucosal damage and cell infiltration. Moreover, enhanced mucosal antibody production, induction of T helper (Th)1 and Th17-related immune responses, enlarged the population of CD8+ cells, and elevated vaginal microflora diversity were observed in vaccinated mice. Interestingly, the potency was rather attenuated when PO@NE was replaced with PP@NE. Conclusion These findings indicate PO@NE as a HK-CA vaccine adjuvant for candidal vaginitis prevention via enhancement of both cellular and humoral immunity and modulation of vaginal microflora, emphasizing further intravaginal vaccination development.
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Affiliation(s)
- Wei-Chung Tsai
- Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan
| | - Fang-Ling Liu
- Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan
| | - Ming-Hsi Huang
- National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan
| | - Chung-Hsiung Huang
- Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan
- Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan
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3
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Radaic A, Shamir ER, Jones K, Villa A, Garud NR, Tward AD, Kamarajan P, Kapila YL. Specific Oral Microbial Differences in Proteobacteria and Bacteroidetes Are Associated with Distinct Sites When Moving from Healthy Mucosa to Oral Dysplasia-A Microbiome and Gene Profiling Study and Focused Review. Microorganisms 2023; 11:2250. [PMID: 37764094 PMCID: PMC10534919 DOI: 10.3390/microorganisms11092250] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/18/2023] [Revised: 08/24/2023] [Accepted: 09/05/2023] [Indexed: 09/29/2023] Open
Abstract
Oral potentially malignant disorders (OPMDs) are a group of conditions that carry a risk of oral squamous cell carcinoma (OSCC) development. Recent studies indicate that periodontal disease-associated pathogenic bacteria may play a role in the transition from healthy mucosa to dysplasia and to OSCC. Yet, the microbial signatures associated with the transition from healthy mucosa to dysplasia have not been established. To characterize oral microbial signatures at these different sites, we performed a 16S sequencing analysis of both oral swab and formalin-fixed, paraffin-embedded tissue (FFPE) samples. We collected oral swabs from healthy mucosa (from healthy patients), histologically normal mucosa adjacent to dysplasia, and low-grade oral dysplasia. Additionally, FFPE samples from histologically normal mucosa adjacent to OSCC, plus low grade and high-grade oral dysplasia samples were also collected. The collected data demonstrate significant differences in the alpha and beta microbial diversities of different sites in oral mucosa, dysplasia, and OSCC, as well as increased dissimilarities within these sites. We found that the Proteobacteria phyla abundance increased, concurrent with a progressive decrease in the Firmicutes phyla abundance, as well as altered levels of Enterococcus cecorum, Fusobacterium periodonticum, Prevotella melaninogenica, and Fusobacterium canifelinum when moving from healthy to diseased sites. Moreover, the swab sample analysis indicates that the oral microbiome may be altered in areas that are histologically normal, including in mucosa adjacent to dysplasia. Furthermore, trends in specific microbiome changes in oral swab samples preceded those in the tissues, signifying early detection opportunities for clinical diagnosis. In addition, we evaluated the gene expression profile of OSCC cells (HSC-3) infected with either P. gingivalis, T. denticola, F. nucelatum, or S. sanguinis and found that the three periodontopathogens enrich genetic processes related to cancer progression, including skin keratinization/cornification, while the commensal enriched processes related to RNA processing and adhesion. Finally, we reviewed the dysplasia microbiome literature and found a significant decrease in commensal bacteria, such as the Streptococci genus, and a simultaneous increase in pathogenic bacteria, mainly Bacteroidetes phyla and Fusobacterium genus. These findings suggest that features of the oral microbiome can serve as novel biomarkers for dysplasia and OSCC disease progression.
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Affiliation(s)
- Allan Radaic
- School of Dentistry, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA; (A.R.); (P.K.)
- School of Dentistry, University of California, San Francisco (UCSF), San Francisco, CA 94143, USA; (K.J.); (A.V.)
| | - Eliah R. Shamir
- School of Medicine, University of California, San Francisco (UCSF), San Francisco, CA 94143, USA; (E.R.S.); (A.D.T.)
- Genentech, Inc., South San Francisco, CA 94080, USA
| | - Kyle Jones
- School of Dentistry, University of California, San Francisco (UCSF), San Francisco, CA 94143, USA; (K.J.); (A.V.)
- Genentech, Inc., South San Francisco, CA 94080, USA
| | - Alessandro Villa
- School of Dentistry, University of California, San Francisco (UCSF), San Francisco, CA 94143, USA; (K.J.); (A.V.)
- Miami Cancer Institute, Baptist Health South Florida, Miami, FL 33176, USA
| | - Nandita R. Garud
- College of Life Sciences, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA;
- Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA
| | - Aaron D. Tward
- School of Medicine, University of California, San Francisco (UCSF), San Francisco, CA 94143, USA; (E.R.S.); (A.D.T.)
| | - Pachiyappan Kamarajan
- School of Dentistry, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA; (A.R.); (P.K.)
- School of Dentistry, University of California, San Francisco (UCSF), San Francisco, CA 94143, USA; (K.J.); (A.V.)
| | - Yvonne L. Kapila
- School of Dentistry, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA; (A.R.); (P.K.)
- School of Dentistry, University of California, San Francisco (UCSF), San Francisco, CA 94143, USA; (K.J.); (A.V.)
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4
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Saraneva O, Furuholm J, Hagström J, Sorsa T, Rita V, Tervahartiala T, Välimaa H, Ruokonen H. Oral Potentially Malignant Disorders and Candida in Oral Tongue Squamous Cell Carcinoma Patients. Dent J (Basel) 2023; 11:170. [PMID: 37504236 PMCID: PMC10377968 DOI: 10.3390/dj11070170] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/07/2023] [Revised: 07/10/2023] [Accepted: 07/12/2023] [Indexed: 07/29/2023] Open
Abstract
This retrospective study addressed the role of oral potentially malignant disorders and the presence of intraepithelial Candida hyphae in the carcinogenesis of the oral tongue squamous cell carcinoma and its association with smoking, alcohol consumption, and oral inflammatory burden. The medical records of 183 subjects diagnosed with oral tongue squamous cell carcinoma at the Helsinki University Hospital were investigated. Preceding oral lichen planus, lichenoid reaction, and leukoplakia diagnosis were recorded. Further, the data on Candida hyphae in histological samples as an indicator of oral candidiasis, oral inflammatory burden, smoking, and alcohol consumption were recorded and analyzed. The histopathological diagnosis of oral lichen planus/lichenoid reaction (p < 0.001) and the presence of Candida hyphae (p = 0.005) were associated significantly with female gender. Oral lichen planus/lichenoid reaction patients were less often smokers than patients without these lesions. Candida hyphae were more often recorded in patients without alcohol use (p = 0.012). Oral lichen planus/lichenoid reaction and Candida hyphae in histological samples were associated with female gender and lower levels of typical risk factors, such as alcohol use and smoking, in oral tongue squamous cell carcinoma patients. Therefore, these patients should be well monitored despite a potential lack of the classical risk factors of oral carcinoma.
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Affiliation(s)
- Orvokki Saraneva
- Department of Oral and Maxillofacial Diseases, University of Helsinki, FI-00290 Helsinki, Finland
- Department of Oral and Maxillofacial Diseases, Helsinki University Hospital and University of Helsinki, FI-00290 Helsinki, Finland
| | - Jussi Furuholm
- Department of Oral and Maxillofacial Diseases, University of Helsinki, FI-00290 Helsinki, Finland
| | - Jaana Hagström
- Department of Pathology, University of Helsinki and Helsinki University Hospital, FI-00014 Helsinki, Finland
- Department of Oral Pathology and Radiology, University of Turku, FI-20520 Turku, Finland
| | - Timo Sorsa
- Department of Oral and Maxillofacial Diseases, University of Helsinki, FI-00290 Helsinki, Finland
- Department of Oral and Maxillofacial Diseases, Helsinki University Hospital and University of Helsinki, FI-00290 Helsinki, Finland
- Division of Periodontology, Department of Dental Medicine, Karolinska Institute, SE-17177 Stockholm, Sweden
| | - Ville Rita
- Department of Oral and Maxillofacial Diseases, University of Helsinki, FI-00290 Helsinki, Finland
| | - Taina Tervahartiala
- Department of Oral and Maxillofacial Diseases, University of Helsinki, FI-00290 Helsinki, Finland
| | - Hannamari Välimaa
- Department of Oral and Maxillofacial Diseases, Helsinki University Hospital and University of Helsinki, FI-00290 Helsinki, Finland
- Department of Virology, University of Helsinki, FI-00290 Helsinki, Finland
- Meilahti Infectious Diseases and Vaccine Research Center, MeVac, Helsinki University Hospital and University of Helsinki, FI-00290 Helsinki, Finland
| | - Hellevi Ruokonen
- Department of Oral and Maxillofacial Diseases, University of Helsinki, FI-00290 Helsinki, Finland
- Department of Oral and Maxillofacial Diseases, Helsinki University Hospital and University of Helsinki, FI-00290 Helsinki, Finland
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5
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Abstract
The microbiome may impact cancer development, progression and treatment responsiveness, but its fungal components remain insufficiently studied in this context. In this review, we highlight accumulating evidence suggesting a possible involvement of commensal and pathogenic fungi in modulation of cancer-related processes. We discuss the mechanisms by which fungi can influence tumour biology, locally by activity exerted within the tumour microenvironment, or remotely through secretion of bioactive metabolites, modulation of host immunity and communications with neighbouring bacterial commensals. We examine prospects of utilising fungi-related molecular signatures in cancer diagnosis, patient stratification and assessment of treatment responsiveness, while highlighting challenges and limitations faced in performing such research. In all, we demonstrate that fungi likely constitute important members of mucosal and tumour-residing microbiomes. Exploration of fungal inter-kingdom interactions with the bacterial microbiome and the host and decoding of their causal impacts on tumour biology may enable their harnessing into cancer diagnosis and treatment.
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Affiliation(s)
- Aurelia Saftien
- Microbiome and Cancer Division, German Cancer Research Center (DKFZ), Heidelberg, Germany
- Faculty of Biosciences, Heidelberg University, Heidelberg, Germany
| | - Jens Puschhof
- Microbiome and Cancer Division, German Cancer Research Center (DKFZ), Heidelberg, Germany
| | - Eran Elinav
- Microbiome and Cancer Division, German Cancer Research Center (DKFZ), Heidelberg, Germany
- Department of Systems Immunology, Weizmann Institute of Science, Rehovot, Israel
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6
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Edel J, Grinstein-Koren O, Winocur-Arias O, Kaplan I, Schnaiderman-Shapiro A, Buchner A, Vered M, Zlotogorski-Hurvitz A. Unexpected Candidal Hyphae in Oral Mucosa Lesions-A Clinico-Pathological Study. Antibiotics (Basel) 2022; 11:antibiotics11101386. [PMID: 36290044 PMCID: PMC9598629 DOI: 10.3390/antibiotics11101386] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/06/2022] [Revised: 10/05/2022] [Accepted: 10/07/2022] [Indexed: 11/16/2022] Open
Abstract
Background: Oral mucosal biopsies might harbor candidal hyphae (CH) in the absence of any clinical signs or symptoms. Aim: To assess oral mucosa biopsies for the frequency of unexpected CH and characterize their clinico-pathological features. Materials and Methods: All biopsy reports (2004−2019) were searched using CH/candida/candidiasis as key words. Cases with clinical diagnosis of oral candidiasis (OC) were excluded. Demographic data, health status, smoking habits, clinical features and diagnoses were collected. Statistical analysis included the chi-square test; significance was set at p < 0.05. Results: Of all the biopsies, 100 (1.05%) reported microscopical evidence of CH without typical clinical signs/symptoms of OC. Fifteen cases were from healthy, non-smoking patients. CH was common on buccal mucosa (38%) and lateral tongue (23%). The tip of tongue (OR = 54.5, 95% CI 9.02−329.4, p < 0.001) and lateral tongue (OR = 3.83, 95% CI 2.4−6.09, p < 0.001) were more likely to harbor CH-positive lesions. CH-positive lesions were diagnosed as epithelial hyperplasia (55%) and exophytic reactive lesions (30%). No correlation was found between CH and the grade of epithelial dysplasia. Conclusions: Microscopic evidence of CH embedded into oral epithelium without typical signs/symptoms of OC is rare, especially in healthy, non-smokers. Since CH was occasionally found in oral sites prone to local trauma and in association with reactive lesions, in absence of host co-morbidities, the contribution of local mechanical forces to CH embedment cannot be ruled out.
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Affiliation(s)
- Jeremy Edel
- Department of Oral Pathology, Oral Medicine and Maxillofacial Imaging, Goldschleger School of Dental Medicine, Tel-Aviv University, Tel-Aviv 6997801, Israel
| | - Osnat Grinstein-Koren
- Department of Oral Pathology, Oral Medicine and Maxillofacial Imaging, Goldschleger School of Dental Medicine, Tel-Aviv University, Tel-Aviv 6997801, Israel
| | - Orit Winocur-Arias
- Department of Oral Pathology, Oral Medicine and Maxillofacial Imaging, Goldschleger School of Dental Medicine, Tel-Aviv University, Tel-Aviv 6997801, Israel
| | - Ilana Kaplan
- Department of Oral Pathology, Oral Medicine and Maxillofacial Imaging, Goldschleger School of Dental Medicine, Tel-Aviv University, Tel-Aviv 6997801, Israel
- Institute of Pathology, Rabin Medical Center, Petach Tikva 4941492, Israel
| | - Anna Schnaiderman-Shapiro
- Department of Oral Pathology, Oral Medicine and Maxillofacial Imaging, Goldschleger School of Dental Medicine, Tel-Aviv University, Tel-Aviv 6997801, Israel
- Institute of Pathology, Sheba Medical Center, Tel Hashomer, Ramat Gan 5262000, Israel
| | - Amos Buchner
- Department of Oral Pathology, Oral Medicine and Maxillofacial Imaging, Goldschleger School of Dental Medicine, Tel-Aviv University, Tel-Aviv 6997801, Israel
| | - Marilena Vered
- Department of Oral Pathology, Oral Medicine and Maxillofacial Imaging, Goldschleger School of Dental Medicine, Tel-Aviv University, Tel-Aviv 6997801, Israel
- Institute of Pathology, Sheba Medical Center, Tel Hashomer, Ramat Gan 5262000, Israel
| | - Ayelet Zlotogorski-Hurvitz
- Department of Oral Pathology, Oral Medicine and Maxillofacial Imaging, Goldschleger School of Dental Medicine, Tel-Aviv University, Tel-Aviv 6997801, Israel
- Department of Oral and Maxillofacial Surgery, Rabin Medical Center, Petach Tikva 4941492, Israel
- Correspondence:
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7
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Theofilou VI, Alfaifi A, Montelongo-Jauregui D, Pettas E, Georgaki M, Nikitakis NG, Jabra-Rizk MA, Sultan AS. The oral mycobiome: Oral epithelial dysplasia and oral squamous cell carcinoma. J Oral Pathol Med 2022; 51:413-420. [PMID: 35347760 DOI: 10.1111/jop.13295] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/18/2021] [Revised: 02/16/2022] [Accepted: 03/20/2022] [Indexed: 12/16/2022]
Abstract
Fungi, a diverse group of eukaryotic organisms, play distinct roles in health and disease. Recent advances in the field of mycobiology have enabled the characterization of the "human mycobiome." The human mycobiome has extensively been studied in various disease models. However, to date, the role of the oral mycobiome in oral carcinogenesis has yet to be elucidated. Candida albicans, the most common oral colonizer, has been speculated to display tumorigenic effects; however, the literature lacks consistent documentation from mechanistic studies on whether oral mycobiota act as drivers, facilitators, or passive colonizers of oral premalignancy and cancer. This review article provides an overview of existing hypothesis-driven mechanistic models that outline the complex interplay between the oral mycobiome and oral epithelial dysplasia as well as their potential clinical implications.
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Affiliation(s)
- Vasileios Ionas Theofilou
- Department of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, Maryland, USA.,Department of Oral Medicine & Pathology and Hospital Dentistry, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece
| | - Areej Alfaifi
- Department of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, Maryland, USA.,Department of Restorative and Prosthetic Dental Sciences, College of Dentistry King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia
| | - Daniel Montelongo-Jauregui
- Department of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, Maryland, USA
| | - Efstathios Pettas
- Department of Oral Medicine & Pathology and Hospital Dentistry, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece
| | - Maria Georgaki
- Department of Oral Medicine & Pathology and Hospital Dentistry, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece
| | - Nikolaos G Nikitakis
- Department of Oral Medicine & Pathology and Hospital Dentistry, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece
| | - Mary-Ann Jabra-Rizk
- Department of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, Maryland, USA.,Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, Maryland, USA
| | - Ahmed S Sultan
- Department of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, Maryland, USA.,Program in Oncology, University of Maryland Greenebaum Cancer Center, Baltimore, Maryland, USA
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8
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Sultan AS, Theofilou VI, Alfaifi A, Montelongo-Jauregui D, Jabra-Rizk MA. Is Candida albicans an opportunistic oncogenic pathogen? PLoS Pathog 2022; 18:e1010413. [PMID: 35421207 PMCID: PMC9009622 DOI: 10.1371/journal.ppat.1010413] [Citation(s) in RCA: 11] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/18/2022] Open
Affiliation(s)
- Ahmed S. Sultan
- Department of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, Maryland, United States of America
- University of Maryland Greenebaum Cancer Center, Baltimore, Maryland, United States of America
- * E-mail:
| | - Vasileios Ionas Theofilou
- Department of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, Maryland, United States of America
| | - Areej Alfaifi
- Department of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, Maryland, United States of America
- Department of Restorative and Prosthetic Dental Sciences, College of Dentistry King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia
| | - Daniel Montelongo-Jauregui
- Department of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, Maryland, United States of America
| | - Mary-Ann Jabra-Rizk
- Department of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, Maryland, United States of America
- Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, Maryland, United States of America
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9
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Mohamed N, Litlekalsøy J, Ahmed IA, Martinsen EMH, Furriol J, Javier-Lopez R, Elsheikh M, Gaafar NM, Morgado L, Mundra S, Johannessen AC, Osman TAH, Nginamau ES, Suleiman A, Costea DE. Analysis of Salivary Mycobiome in a Cohort of Oral Squamous Cell Carcinoma Patients From Sudan Identifies Higher Salivary Carriage of Malassezia as an Independent and Favorable Predictor of Overall Survival. Front Cell Infect Microbiol 2021; 11:673465. [PMID: 34712619 PMCID: PMC8547610 DOI: 10.3389/fcimb.2021.673465] [Citation(s) in RCA: 41] [Impact Index Per Article: 10.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/27/2021] [Accepted: 08/27/2021] [Indexed: 12/20/2022] Open
Abstract
Background Microbial dysbiosis and microbiome-induced inflammation have emerged as important factors in oral squamous cell carcinoma (OSCC) tumorigenesis during the last two decades. However, the “rare biosphere” of the oral microbiome, including fungi, has been sparsely investigated. This study aimed to characterize the salivary mycobiome in a prospective Sudanese cohort of OSCC patients and to explore patterns of diversities associated with overall survival (OS). Materials and Methods Unstimulated saliva samples (n = 72) were collected from patients diagnosed with OSCC (n = 59) and from non-OSCC control volunteers (n = 13). DNA was extracted using a combined enzymatic–mechanical extraction protocol. The salivary mycobiome was assessed using a next-generation sequencing (NGS)-based methodology by amplifying the ITS2 region. The impact of the abundance of different fungal genera on the survival of OSCC patients was analyzed using Kaplan–Meier and Cox regression survival analyses (SPPS). Results Sixteen genera were identified exclusively in the saliva of OSCC patients. Candida, Malassezia, Saccharomyces, Aspergillus, and Cyberlindnera were the most relatively abundant fungal genera in both groups and showed higher abundance in OSCC patients. Kaplan–Meier survival analysis showed higher salivary carriage of the Candida genus significantly associated with poor OS of OSCC patients (Breslow test: p = 0.043). In contrast, the higher salivary carriage of Malassezia showed a significant association with favorable OS in OSCC patients (Breslow test: p = 0.039). The Cox proportional hazards multiple regression model was applied to adjust the salivary carriage of both Candida and Malassezia according to age (p = 0.029) and identified the genus Malassezia as an independent predictor of OS (hazard ratio = 0.383, 95% CI = 0.16–0.93, p = 0.03). Conclusion The fungal compositional patterns in saliva from OSCC patients were different from those of individuals without OSCC. The fungal genus Malassezia was identified as a putative prognostic biomarker and therapeutic target for OSCC.
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Affiliation(s)
- Nazar Mohamed
- Gade Laboratory for Pathology, Department of Clinical Medicine, and Center for Cancer Biomarkers CCBIO, University of Bergen, Bergen, Norway.,Department of Oral and Maxillofacial Surgery/Department of Basic Sciences, University of Khartoum, Khartoum, Sudan
| | - Jorunn Litlekalsøy
- Gade Laboratory for Pathology, Department of Clinical Medicine, and Center for Cancer Biomarkers CCBIO, University of Bergen, Bergen, Norway
| | - Israa Abdulrahman Ahmed
- Gade Laboratory for Pathology, Department of Clinical Medicine, and Center for Cancer Biomarkers CCBIO, University of Bergen, Bergen, Norway.,Department of Operative Dentistry, University of Science & Technology, Omdurman, Sudan
| | | | - Jessica Furriol
- Department of Nephrology, Haukeland University Hospital, Bergen, Norway
| | - Ruben Javier-Lopez
- Department of Biological Sciences, The Faculty of Mathematics and Natural Sciences, University of Bergen, Bergen, Norway
| | - Mariam Elsheikh
- Department of Oral and Maxillofacial Surgery/Department of Basic Sciences, University of Khartoum, Khartoum, Sudan.,Department of Oral & Maxillofacial Surgery, Khartoum Dental Teaching Hospital, Khartoum, Sudan
| | - Nuha Mohamed Gaafar
- Gade Laboratory for Pathology, Department of Clinical Medicine, and Center for Cancer Biomarkers CCBIO, University of Bergen, Bergen, Norway.,Department of Oral and Maxillofacial Surgery/Department of Basic Sciences, University of Khartoum, Khartoum, Sudan
| | - Luis Morgado
- Section for Genetics and Evolutionary Biology (EvoGene), Department of Biosciences, The Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway
| | - Sunil Mundra
- Section for Genetics and Evolutionary Biology (EvoGene), Department of Biosciences, The Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway.,Department of Biology, College of Science, United Arab Emirates University, Al Ain, Abu Dhabi, United Arab Emirates
| | - Anne Christine Johannessen
- Gade Laboratory for Pathology, Department of Clinical Medicine, and Center for Cancer Biomarkers CCBIO, University of Bergen, Bergen, Norway.,Department of Pathology, Laboratory Clinic, Haukeland University Hospital, Bergen, Norway
| | - Tarig Al-Hadi Osman
- Gade Laboratory for Pathology, Department of Clinical Medicine, and Center for Cancer Biomarkers CCBIO, University of Bergen, Bergen, Norway
| | - Elisabeth Sivy Nginamau
- Gade Laboratory for Pathology, Department of Clinical Medicine, and Center for Cancer Biomarkers CCBIO, University of Bergen, Bergen, Norway.,Department of Pathology, Laboratory Clinic, Haukeland University Hospital, Bergen, Norway
| | - Ahmed Suleiman
- Department of Oral and Maxillofacial Surgery/Department of Basic Sciences, University of Khartoum, Khartoum, Sudan.,Department of Oral & Maxillofacial Surgery, Khartoum Dental Teaching Hospital, Khartoum, Sudan
| | - Daniela Elena Costea
- Gade Laboratory for Pathology, Department of Clinical Medicine, and Center for Cancer Biomarkers CCBIO, University of Bergen, Bergen, Norway.,Department of Pathology, Laboratory Clinic, Haukeland University Hospital, Bergen, Norway
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Handschuh Briones RA, Silva Arcos EN, Urrutia M, Godoy-Martínez P. Actividad antifúngica de los enjuagues bucales frente a Candida albicans y Rhodotorula mucilaginosa: un estudio in vitro. Rev Iberoam Micol 2020; 37:47-52. [DOI: 10.1016/j.riam.2019.10.006] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/20/2018] [Revised: 10/03/2019] [Accepted: 10/30/2019] [Indexed: 10/23/2022] Open
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Shukla K, Vun I, Lov I, Laparidis G, McCamley C, Ariyawardana A. Role of Candida infection in the malignant transformation of oral leukoplakia: A systematic review of observational studies. TRANSLATIONAL RESEARCH IN ORAL ONCOLOGY 2019. [DOI: 10.1177/2057178x19828229] [Citation(s) in RCA: 15] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022] Open
Abstract
The aim of this systematic review was to investigate the malignant transformation of oral leukoplakia (OL) infected with Candida. Literature search was conducted using Medical Subject Heading terms ‘leukoplakia’, ‘neoplasms’, ‘ Candida’, ‘malignant transformation’ and ‘oral’ and included all results until February 2017. The initial search identified 372 articles, which was consolidated to 16 articles after applying inclusion and exclusion criteria. The occurrence of candidal infection in OL ranged from 6.8% to 100.0%. Only 3 of 16 studies reported malignant transformation rates of leukoplakia with Candida infection. The overall malignant transformation rates reported in these studies were 2.5%, 6.5% and 28.7%. This review supports the contention that candidal infection promotes cellular and dysplastic changes in OL. However, further follow-up studies are recommended to confirm the role of Candida infection in malignant transformation of OL lesions.
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Affiliation(s)
- Khilan Shukla
- Division of Tropical Health and Medicine, College of Medicine and Dentistry, James Cook University, Smithfield, QLD, Australia
| | - Ida Vun
- Division of Tropical Health and Medicine, College of Medicine and Dentistry, James Cook University, Smithfield, QLD, Australia
| | - Ivan Lov
- Division of Tropical Health and Medicine, College of Medicine and Dentistry, James Cook University, Smithfield, QLD, Australia
| | - George Laparidis
- Division of Tropical Health and Medicine, College of Medicine and Dentistry, James Cook University, Smithfield, QLD, Australia
| | - Caitlin McCamley
- Division of Tropical Health and Medicine, College of Medicine and Dentistry, James Cook University, Smithfield, QLD, Australia
| | - Anura Ariyawardana
- Division of Tropical Health and Medicine, College of Medicine and Dentistry, James Cook University, Smithfield, QLD, Australia
- Metro South Oral Health, Queensland Health, Brisbane, QLD, Australia
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12
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Farah CS, Pollaers K, Frydrych A. Management of Premalignant Disease of the Oral Mucosa. HEAD AND NECK CANCER CLINICS 2019. [DOI: 10.1007/978-981-13-2931-9_11] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/26/2022]
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14
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Decsi G, Soki J, Pap B, Dobra G, Harmati M, Kormondi S, Pankotai T, Braunitzer G, Minarovits J, Sonkodi I, Urban E, Nemeth IB, Nagy K, Buzas K. Chicken or the Egg: Microbial Alterations in Biopsy Samples of Patients with Oral Potentially Malignant Disorders. Pathol Oncol Res 2018; 25:1023-1033. [PMID: 30054809 DOI: 10.1007/s12253-018-0457-x] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 06/08/2018] [Accepted: 07/19/2018] [Indexed: 12/23/2022]
Abstract
Oral carcinogenesis often leads to the alteration of the microbiota at the site of the tumor, but data are scarce regarding the microbial communities of oral potentially malignant disorders (OPMDs). Punch biopsies were taken from healthy and non-healthy mucosa of OPMD patients to analyze the microbiome using metagenome sequencing. In healthy oral mucosa biopsies the bacterial phyla Firmicutes, Fusobacteria, Proteobacteria, Actinobacteria and Bacteroidetes were detected by Ion Torrent sequencing. The same phyla as well as the phyla Fibrobacteres and Spirochaetes were present in the OPMD biopsies. On the species level, there were 10 bacterial species unique to the healthy tissue and 35 species unique to the OPMD lesions whereas eight species were detected in both samples. We observed that the relative abundance of Streptococcus mitis decreased in the OPMD lesions compared to the uninvolved tissue. In contrast, the relative abundance of Fusobacterium nucleatum, implicated in carcinogenesis, was elevated in OPMD. We detected markedly increased bacterial diversity in the OPMD lesions compared to the healthy oral mucosa. The ratio of S. mitis and F. nucleatum are characteristically altered in the OPMD lesions compared to the healthy mucosa.
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Affiliation(s)
- Gabor Decsi
- Faculty of Dentistry, Department of Oral Surgery, University of Szeged, Tisza Lajos krt. 64, Szeged, H-6720, Hungary
| | - Jozsef Soki
- Albert Szent-Gyorgyi Clinical Centre, Institute of Clinical Microbiology, University of Szeged, Semmelweis u. 6, Szeged, H-6725, Hungary
| | - Bernadett Pap
- Biological Research Centre, Hungarian Academy of Sciences, Temesvari krt. 62, Szeged, H-6726, Hungary
| | - Gabriella Dobra
- Biological Research Centre, Hungarian Academy of Sciences, Temesvari krt. 62, Szeged, H-6726, Hungary
| | - Maria Harmati
- Biological Research Centre, Hungarian Academy of Sciences, Temesvari krt. 62, Szeged, H-6726, Hungary
| | - Sandor Kormondi
- Albert Szent-Gyorgyi Clinical Centre, Department of Traumatology, University of Szeged, Semmelweis u. 6, Szeged, H-6725, Hungary
| | - Tibor Pankotai
- Department of Biochemistry and Molecular Biology, University of Szeged, Kozep fasor 52, Szeged, H-6726, Hungary
| | | | - Janos Minarovits
- Faculty of Dentistry, Department of Oral Biology and Experimental Dental Research, University of Szeged, Tisza Lajos krt. 64, Szeged, H-6720, Hungary
| | - Istvan Sonkodi
- Faculty of Dentistry, Department of Oral Surgery, University of Szeged, Tisza Lajos krt. 64, Szeged, H-6720, Hungary
| | - Edit Urban
- Albert Szent-Gyorgyi Clinical Centre, Institute of Clinical Microbiology, University of Szeged, Semmelweis u. 6, Szeged, H-6725, Hungary
| | - Istvan Balazs Nemeth
- Department of Dermatology and Allergology, University of Szeged, Szeged, H-6720, Hungary
| | - Katalin Nagy
- Faculty of Dentistry, Department of Oral Surgery, University of Szeged, Tisza Lajos krt. 64, Szeged, H-6720, Hungary
| | - Krisztina Buzas
- Biological Research Centre, Hungarian Academy of Sciences, Temesvari krt. 62, Szeged, H-6726, Hungary. .,Faculty of Dentistry, Department of Oral Biology and Experimental Dental Research, University of Szeged, Tisza Lajos krt. 64, Szeged, H-6720, Hungary.
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Hulimane S, Maluvadi-Krishnappa R, Mulki S, Rai H, Dayakar A, Kabbinahalli M. Speciation of Candida using CHROMagar in cases with oral epithelial dysplasia and squamous cell carcinoma. J Clin Exp Dent 2018; 10:e657-e660. [PMID: 30057706 PMCID: PMC6057081 DOI: 10.4317/jced.54737] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/17/2018] [Accepted: 04/26/2018] [Indexed: 12/29/2022] Open
Abstract
Background Candida albicans is most frequently isolated from oral cavity but identification of other Candida species such as C. tropicalis, C. krusei, C. glabrata & C. dubliniensis is increasing proportionately. A constant rise in immuno-suppressed patients, widening range of recognized pathogens, and resistance to antifungal drugs are contributing factors which stress the need for species identification of Candida, an opportunistic pathogen. Objectives: 1. To detect the prevalence of Candida albicans and Non albicans Candida albicans (NAC) species in the oral cavity of patients with epithelial dysplasia, Oral squamous cell carcinoma (OSCC) and healthy controls. 2. To identify and differentiate Candidal species using CHROMagar, a differential media. Material and Methods The study included smears from 50 patients with histopathological confirmation of epithelial dysplasia & OSCC and 50 normal controls. Candida albicans was identified using Sabouraud dextrose agar media (SDA) as primary culture followed by species identification using CHROMagar on the basis of colony color and morphology. Results Non albicans candida predominated (66%) over Candida albicans (34%) in speciation on CHROMagar media in the study group. Non albicans Candida species isolated were C. tropicalis (38%), C. glabrata (24%) and 2 cases showing polyfungal population of C. albicans & C. glabrata. Conclusions Species level isolation of Candida helps in early identification of resistant non Candida strains and prompt treatment of the cases there by preventing the dissemination of infection in case of immuno-compromised individuals. The data presented also supports the use of CHROMagar Candida as a pertinent media for the rapid identification of Candida species directly from clinical specimens in resource challenged settings, which could be helpful in developing appropriate therapeutic strategy and management of patients. Key words:Candida, CHROMagar, epithelial dysplasia, oral cancer.
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Affiliation(s)
- Supriya Hulimane
- Senior lecturer, Department of Oral Pathology and Microbiology, KVG Dental College and Hospital, Sullia - 574 237, D.K, Karnataka, India
| | - Ramya Maluvadi-Krishnappa
- Senior lecturer, Department of Oral Pathology and Microbiology, KVG Dental College and Hospital, Sullia - 574 237, D.K, Karnataka, India
| | - Shaila Mulki
- Senior lecturer, Department of Oral Pathology and Microbiology, KVG Dental College and Hospital, Sullia - 574 237, D.K, Karnataka, India
| | - Harishchandra Rai
- Senior lecturer, Department of Oral Pathology and Microbiology, KVG Dental College and Hospital, Sullia - 574 237, D.K, Karnataka, India
| | - Anitha Dayakar
- Senior lecturer, Department of Oral Pathology and Microbiology, KVG Dental College and Hospital, Sullia - 574 237, D.K, Karnataka, India
| | - Meghashree Kabbinahalli
- Senior lecturer, Department of Oral Pathology and Microbiology, KVG Dental College and Hospital, Sullia - 574 237, D.K, Karnataka, India
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Inhibitory Activities of Herbal Based Toothpaste on Germ Tube and Adhesion of Candida albicans. JOURNAL OF PURE AND APPLIED MICROBIOLOGY 2016. [DOI: 10.22207/jpam.10.4.09] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022] Open
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17
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Gönen ZB, Yılmaz Asan C, Etöz O, Alkan A. Oral leukoplakia associated with amalgam restorations. J Oral Sci 2016; 58:445-8. [PMID: 27665986 DOI: 10.2334/josnusd.16-0071] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/01/2022]
Abstract
The World Health Organization has defined oral leukoplakia (OL) as "a white patch or plaque of the oral mucosa that cannot be characterized clinically or pathologically as any other disease". A 21-year-old male with OL presented with a bilateral burning sensation in the buccal mucosa. The patient had amalgam restorations, and an epicutaneous patch test indicated a positive response to amalgam. The amalgam restorations were therefore removed and the cavities were refilled with a composite resin restorative material. During 5 years of follow-up, there was no recurrence of the oral lesions. This case illustrates that amalgam fillings may cause OL lesions. (J Oral Sci 58, 445-448, 2016).
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Affiliation(s)
- Zeynep B Gönen
- Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Erciyes University
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18
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Berkovits C, Tóth A, Szenzenstein J, Deák T, Urbán E, Gácser A, Nagy K. Analysis of oral yeast microflora in patients with oral squamous cell carcinoma. SPRINGERPLUS 2016; 5:1257. [PMID: 27536540 PMCID: PMC4974209 DOI: 10.1186/s40064-016-2926-6] [Citation(s) in RCA: 18] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 12/20/2015] [Accepted: 07/27/2016] [Indexed: 12/02/2022]
Abstract
Background Oral squamous cell carcinoma (OSCC) is the most common form of oral cancer, in this study, the association between OSCC and oral yeast carriage was investigated. Findings 20 patients having OSCC as well as 40 healthy controls were tested for the presence of yeasts in the oral cavity. Fungal burdens were examined by colony forming unit determinations, while the different yeast genera in patient samples were identified by matrix-associated laser desorption/ionization-time of flight-mass spectrometry. We found that the level of oral yeast carriage was significantly higher in patients with OSCC that was accompanied by a higher diversity of yeasts in the oral cavity of these patients. We also examined the extracellular enzyme production of isolated Candida spp.; however, we found that there was no association between the lipase/protease producing capacity of Candida strains and the higher colonisation rate of neoplastic epithelium. Conclusions In conclusion, our results corroborate the findings of previous studies regarding the association between oral yeast carriage and epithelial carcinoma.
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Affiliation(s)
- Csaba Berkovits
- Department of Oral Surgery, University of Szeged, Tisza Lajos krt. 64, Szeged, 6720 Hungary
| | - Adél Tóth
- Department of Microbiology, University of Szeged, Közép fasor 52, Szeged, 6726 Hungary
| | - Judit Szenzenstein
- Department of Microbiology, University of Szeged, Közép fasor 52, Szeged, 6726 Hungary
| | - Tünde Deák
- Institute of Clinical Microbiology, University of Szeged, Semmelweis u. 6, Szeged, 6725 Hungary
| | - Edit Urbán
- Institute of Clinical Microbiology, University of Szeged, Semmelweis u. 6, Szeged, 6725 Hungary
| | - Attila Gácser
- Department of Microbiology, University of Szeged, Közép fasor 52, Szeged, 6726 Hungary
| | - Katalin Nagy
- Department of Oral Surgery, University of Szeged, Tisza Lajos krt. 64, Szeged, 6720 Hungary
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Long-term outcome of non-surgical treatment in patients with oral leukoplakia. Oral Oncol 2015; 51:1020-1025. [PMID: 26410021 DOI: 10.1016/j.oraloncology.2015.09.004] [Citation(s) in RCA: 54] [Impact Index Per Article: 5.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/07/2015] [Revised: 09/03/2015] [Accepted: 09/04/2015] [Indexed: 11/20/2022]
Abstract
UNLABELLED The standard treatments for oral leukoplakia range from careful observation to complete resection. No surgical intervention is chosen for several supposable reasons. Surgical treatment and no surgical treatment for oral leukoplakia have no defined basis for comparisons, and few studies have reported on the long-term outcomes of oral leukoplakia without surgery. OBJECTIVES This study aimed to identify the important factors using a long-term wait-and-see policy in patients with oral leukoplakia. MATERIALS AND METHODS In total, 237 lesions from 218 patients selected for non-surgical therapy between 2001 and 2010 were analyzed. On the basis of long-term follow-up data, lesions were classified as unchanged, reduced, disappeared, expanded, and malignantly transformed. RESULTS In total, 135 (57.0%) lesions remained unchanged, 30 (12.7%) lesions were characterized by a reduction in size or clinical severity, and 44 (18.6%) lesions had disappeared. Another 17 (7.2%) lesions resulted in spread or clinical deterioration, and 11 (4.6%) lesions developed oral squamous cell carcinoma. CONCLUSIONS We demonstrated a cumulative malignant transformation rate of 11.6% in 10years without resection. The lesions that were nonhomogeneous, and higher degree of epithelial dysplasia, located on the tongue were likely to progress into cancer. In addition, 32.5% of lesions without surgical treatment were reduced or disappeared. There is a possibility that removal of considerable irritation for a long time contributes to the treatment of this disease. The development of appropriate treatments for oral leukoplakia is required, which will enable successful differentiation between surgical and observation cases.
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Kolude BM, Bouquot JE. Clinical pathologic conference case 3: A thick, granular white gingival plaque in an adult male. Oral Surg Oral Med Oral Pathol Oral Radiol 2015; 119:e279-84. [DOI: 10.1016/j.oooo.2014.11.006] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/12/2023]
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Silva-Rocha WP, de Brito Lemos VL, Ferreira MRA, Soares LAL, Svidzisnki TIE, Milan EP, Chaves GM. Effect of the crude extract of Eugenia uniflora in morphogenesis and secretion of hydrolytic enzymes in Candida albicans from the oral cavity of kidney transplant recipients. Altern Ther Health Med 2015; 15:6. [PMID: 25651849 PMCID: PMC4324049 DOI: 10.1186/s12906-015-0522-x] [Citation(s) in RCA: 26] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/20/2014] [Accepted: 01/14/2015] [Indexed: 12/31/2022]
Abstract
Background Candida albicans is a diploid yeast that in some circumstances may cause oral or oropharyngeal infections. Yeasts virulence factors contribute for both the maintenance of colonizing strains in addition to damage and cause tissue invasion, thus the establishment of infection occurs. The limited arsenal of antifungal drugs for the treatment of candidiasis turn the investigation of natural products mandatory for the discovery of new targets for antifungal drug development. Therefore, tropical countries emerge as important providers of natural products with potential antimicrobial activity. This study aimed to investigate morphogenesis and secretion of hydrolytic enzymes (phospholipase and proteinase) in the presence of the CE of Eugenia uniflora. Methods The isolates were tested for their ability to form hyphae in both solid and liquid media under three different conditions: YPD + 20% FBS, Spider medium and GlcNac and the ability to secrete phospholipase and proteinase in the presence of 2000 μg/mL of E. uniflora. Results The CE of E. uniflora inhibited hypha formation in both liquid and solid media tested. It also impaired hydrolytic enzymes production. Conclusions This was the first study to describe the interaction of a natural product with the full expression of three different factors in C. albicans. E. uniflora may be an alternative therapeutic for oral candidiasis in the future.
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Grossmann SDMC, Oliveira CDNAD, Souto GR, Góes C, Mesquita RA. Oral lichenoid lesion: A review of the literature. World J Stomatol 2015; 4:103. [DOI: 10.5321/wjs.v4.i2.103] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/03/2014] [Revised: 01/26/2015] [Accepted: 02/12/2015] [Indexed: 02/06/2023] Open
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