Copyright
©The Author(s) 2024.
World J Orthop. Apr 18, 2024; 15(4): 363-378
Published online Apr 18, 2024. doi: 10.5312/wjo.v15.i4.363
Published online Apr 18, 2024. doi: 10.5312/wjo.v15.i4.363
Stages | Time | Speed | Duration |
Acclimatization treadmill exercise | Day 1 | 6 m/min | 30 min |
Day 2 | 9 m/min | 40 min | |
Day 3 | 12 m/min | 50 min | |
Formal treadmill exercise | Days 4 to 7 | 15 m/min | 60 min |
Weeks 2 to 8 | 15 m/min | 60 min |
Genes | Primer sequence (5'-3') |
lncRNA H19 | Forward: 5'- GCTCCACTGACCTTCTAAAC -3' Reverse: 5'- ACGATGTCTCCTTTGCTAAC -3' |
miR-149 | Forward: 5'- TGGCTCCGTATCTTCACTCC -3' |
β-actin | Forward: 5'-CTGTCCCTGTATGCCTCTG-3' Reverse: 5'-ATGTCACGCACGATTTCC-3' |
ALP | Forward: 5'-CCAACTCTTTTGTGCCAGAGA-3' Reverse: 5'-GGCTACATTGGTGTTGAGCTTTT-3' |
Runx2 | Forward: 5'-AGAGTCAGATTACAGATCCCAGG-3' Reverse: 5'-TGGCTCTTCTTACTGAGAGAGG-3' |
BMP2 | Forward: 5'- GTGCAGATCCTAGGTTTCTCTG-3' Reverse: 5’- CAGGATCTCATTCTCTTGGATC-3’ |
Osterix | Forward: 5'-TGGTACAAGGCAGGCATCCA-3' Reverse: 5'-GGAGCAAAGTCAGATGGGTAAGT-3' |
- Citation: Zhou XC, Wang DX, Zhang CY, Yang YJ, Zhao RB, Liu SY, Ni GX. Exercise promotes osteogenic differentiation by activating the long non-coding RNA H19/microRNA-149 axis. World J Orthop 2024; 15(4): 363-378
- URL: https://www.wjgnet.com/2218-5836/full/v15/i4/363.htm
- DOI: https://dx.doi.org/10.5312/wjo.v15.i4.363