Basic Study
Copyright ©The Author(s) 2024.
World J Orthop. Apr 18, 2024; 15(4): 363-378
Published online Apr 18, 2024. doi: 10.5312/wjo.v15.i4.363
Table 1 Treadmill exercise program
Stages
Time
Speed
Duration
Acclimatization treadmill exerciseDay 16 m/min30 min
Day 29 m/min40 min
Day 312 m/min50 min
Formal treadmill exerciseDays 4 to 715 m/min60 min
Weeks 2 to 815 m/min60 min
Table 2 Primer sequences
Genes
Primer sequence (5'-3')
lncRNA H19Forward: 5'- GCTCCACTGACCTTCTAAAC -3'
Reverse: 5'- ACGATGTCTCCTTTGCTAAC -3'
miR-149Forward: 5'- TGGCTCCGTATCTTCACTCC -3'
β-actinForward: 5'-CTGTCCCTGTATGCCTCTG-3'
Reverse: 5'-ATGTCACGCACGATTTCC-3'
ALPForward: 5'-CCAACTCTTTTGTGCCAGAGA-3'
Reverse: 5'-GGCTACATTGGTGTTGAGCTTTT-3'
Runx2Forward: 5'-AGAGTCAGATTACAGATCCCAGG-3'
Reverse: 5'-TGGCTCTTCTTACTGAGAGAGG-3'
BMP2Forward: 5'- GTGCAGATCCTAGGTTTCTCTG-3'
Reverse: 5’- CAGGATCTCATTCTCTTGGATC-3’
OsterixForward: 5'-TGGTACAAGGCAGGCATCCA-3'
Reverse: 5'-GGAGCAAAGTCAGATGGGTAAGT-3'