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Unal İ. Green Synthesis of Multi-Walled Carbon Nanotube-Reinforced Hydroxyapatite Doped with Silver and Silver-Core Selenium-Shell Nanoparticles: Synthesis, Characterization, and Biological Activity. NANOMATERIALS (BASEL, SWITZERLAND) 2025; 15:179. [PMID: 39940155 PMCID: PMC11820691 DOI: 10.3390/nano15030179] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/26/2024] [Accepted: 01/15/2025] [Indexed: 02/14/2025]
Abstract
Hydroxyapatite (HAp) is widely used in biomedical applications due to its biocompatibility, osteoconductivity, and bioactivity. However, its low mechanical strength, tendency toward rapid corrosion, and lack of bactericidal properties present significant limitations in applications. This study aimed to improve the properties of HAp by reinforcing it with multi-walled carbon nanotubes (MWCNTs) and doping it with silver nanoparticles (AgNPs) and silver-core selenium-shell nanoparticles (Ag@SeNPs). Ocimum basilicum extract was used as both a reducing and stabilizing agent in the synthesis of nanoparticles using an environmentally friendly and non-toxic method as an alternative to traditional methods. The synthesized HAp, HAp/MWCNT, Ag-HAp/MWCNT, and Ag@Se-HAp/MWCNT nanocomposites were characterized by TEM, SEM, XRD, Raman spectroscopy, and BET analysis. BET analysis showed a reduction in surface area from 109.4 m2/g for pure HAp to 71.4 m2/g, 47.5 m2/g, and 35.3 m2/g for HAp/MWCNTs, Ag- HAp/MWCNTs, and Ag@Se-HAp/MWCNTs, respectively. Antimicrobial activities against P. aeruginosa, E. coli, S. aureus, E. faecalis, and C. albicans were evaluated. HAp and HAp/MWCNT did not show any antimicrobial activity, while Ag-HAp/MWCNTs showed inhibition zones of 14 mm for Escherichia coli and 18 mm for Pseudomonas aeruginosa at 5 mg/mL. Ag@Se-MWCNTs/HAp exhibited superior efficacy with inhibition zones of 18 mm, 12 mm, and 20 mm for S. aureus, E. faecalis, and Candida albicans, respectively. The incorporation of Ag@SeNPs enhanced HAp's antibacterial and antifungal properties through a synergistic mechanism.
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Affiliation(s)
- İlkay Unal
- Department of Gastronomy and Culinary Arts, Faculty of Fine Arts, Design and Architecture Education, Munzur University, 62000 Tunceli, Turkey
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2
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Singh I, Dixit K, Gupta P, George SM, Sinha N, Balani K. 3D-Printed Multifunctional Ag/CeO 2/ZnO Reinforced Hydroxyapatite-Based Scaffolds with Effective Antibacterial and Mechanical Properties. ACS APPLIED BIO MATERIALS 2023; 6:5210-5223. [PMID: 37955988 DOI: 10.1021/acsabm.3c00457] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/15/2023]
Abstract
Conventional three-dimensional (3D)-printed hydroxyapatite (HA)-based constructs have limited utility in bone tissue engineering due to their poor mechanical properties, elevated risk of microbial infection, and limited pore interconnectivity. 3D printing of complex multiple components to fabricate fully interconnected scaffolds is a challenging task; here, in this work, we have developed a procedure for fabrication of printable ink for complex systems containing multinanomaterials, i.e., HAACZ (containing 1 wt % Ag, 4 wt % CeO2, and 6 wt % ZnO) with better shear thinning and shape retention properties. Moreover, 3D-printed HAACZ scaffolds showed a modulus of 143.8 GPa, a hardness of 10.8 GPa, a porosity of 59.6%, effective antibacterial properties, and a fully interconnected pore network to be an ideal construct for bone healing. Macropores with an average size of ∼469 and ∼433 μm within the scaffolds of HA and HAACZ and micropores with an average size of ∼0.6 and ∼0.5 μm within the strut of HA and HAACZ were developed. The distribution of fully interconnected micropores was confirmed using computerized tomography, whereas the distribution of micropores within the strut was visualized using Voronoi tessellation. The water contact angle studies revealed the most suitable hydrophilic range of water contact angles of ∼71.7 and ∼76.6° for HA and HAACZ, respectively. HAACZ scaffolds showed comparable apatite formation and cytocompatibility as that of HA. Antibacterial studies revealed effective antibacterial properties for the HAACZ scaffold as compared to HA. There was a decrease in bacterial cell density for HAACZ from 1 × 105 to 1.2 × 103 cells/mm2 against Gram-negative (Escherichia coli) and from 1.9 × 105 to 5.6 × 103 bacterial cells/mm2 against Gram-positive (Staphylococcus aureus). Overall, the 3D-printed HAACZ scaffold resulted in mechanical properties, comparable to those of the cancellous bone, interconnected macro- and microporosities, and excellent antibacterial properties, which could be utilized for bone healing.
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Affiliation(s)
- Indrajeet Singh
- Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India
| | - Kartikeya Dixit
- Department of Mechanical Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India
| | - Pankaj Gupta
- Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India
| | - Suchi Mercy George
- Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India
| | - Niraj Sinha
- Department of Mechanical Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India
| | - Kantesh Balani
- Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India
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3
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Zeng M, Xu Z, Song ZQ, Li JX, Tang ZW, Xiao S, Wen J. Diagnosis and treatment of chronic osteomyelitis based on nanomaterials. World J Orthop 2023; 14:42-54. [PMID: 36844379 PMCID: PMC9945247 DOI: 10.5312/wjo.v14.i2.42] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 10/09/2022] [Revised: 12/01/2022] [Accepted: 01/17/2023] [Indexed: 02/17/2023] Open
Abstract
Chronic osteomyelitis is a painful and serious disease caused by infected surgical prostheses or infected fractures. Traditional treatment includes surgical debridement followed by prolonged systemic antibiotics. However, excessive antibiotic use has been inducing rapid emergence of antibiotic-resistant bacteria worldwide. Additionally, it is difficult for antibiotics to penetrate internal sites of infection such as bone, thus limiting their efficacy. New approaches to treat chronic osteomyelitis remain a major challenge for orthopedic surgeons. Luckily, the development of nanotechnology has brought new antimicrobial options with high specificity to infection sites, offering a possible way to address these challenges. Substantial progress has been made in constructing antibacterial nanomaterials for treatment of chronic osteomyelitis. Here, we review some current strategies for treatment of chronic osteomyelitis and their underlying mechanisms.
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Affiliation(s)
- Ming Zeng
- Department of Pediatric Orthopedics, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 410013, Hunan Province, China
| | - Zheng Xu
- Department of Pediatric Orthopedics, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 410013, Hunan Province, China
| | - Zhen-Qi Song
- Department of Pediatric Orthopedics, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 410013, Hunan Province, China
| | - Jie-Xiao Li
- Department of Pediatric Orthopedics, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 410013, Hunan Province, China
| | - Zhong-Wen Tang
- Department of Pediatric Orthopedics, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 410013, Hunan Province, China
| | - Sheng Xiao
- Department of Pediatric Orthopedics, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 410013, Hunan Province, China
| | - Jie Wen
- Department of Pediatric Orthopedics, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 410013, Hunan Province, China
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4
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Sahoo J, Sarkhel S, Mukherjee N, Jaiswal A. Nanomaterial-Based Antimicrobial Coating for Biomedical Implants: New Age Solution for Biofilm-Associated Infections. ACS OMEGA 2022; 7:45962-45980. [PMID: 36570317 PMCID: PMC9773971 DOI: 10.1021/acsomega.2c06211] [Citation(s) in RCA: 32] [Impact Index Per Article: 10.7] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/26/2022] [Accepted: 11/09/2022] [Indexed: 05/12/2023]
Abstract
Recently, the upsurge in hospital-acquired diseases has put global health at risk. Biomedical implants being the primary source of contamination, the development of biomedical implants with antimicrobial coatings has attracted the attention of a large group of researchers from around the globe. Bacteria develops biofilms on the surface of implants, making it challenging to eradicate them with the standard approach of administering antibiotics. A further issue of current concern is the fast resurgence of resistance to conventional antibiotics. As nanotechnology continues to advance, various types of nanomaterials have been created, including 2D nanoparticles and metal and metal oxide nanoparticles with antimicrobial properties. Researchers from all over the world are using these materials as a coating agent for biomedical implants to create an antimicrobial environment. This comprehensive and contemporary review summarizes various metals, metal oxide nanoparticles, 2D nanomaterials, and their composites that have been used or may be used in the future as an antimicrobial coating agent for biomedical implants, as well as their succinct mode of action to combat biofilm-associated infection and diseases.
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Affiliation(s)
| | | | - Nivedita Mukherjee
- School of Biosciences and Bioengineering, Indian Institute of Technology Mandi, Kamand, Mandi, Himachal Pradesh 175075, India
| | - Amit Jaiswal
- School of Biosciences and Bioengineering, Indian Institute of Technology Mandi, Kamand, Mandi, Himachal Pradesh 175075, India
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Dai X, Thompson EW, Ostrikov K(K. Receptor-Mediated Redox Imbalance: An Emerging Clinical Avenue against Aggressive Cancers. Biomolecules 2022; 12:biom12121880. [PMID: 36551308 PMCID: PMC9775490 DOI: 10.3390/biom12121880] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/14/2022] [Revised: 12/09/2022] [Accepted: 12/13/2022] [Indexed: 12/23/2022] Open
Abstract
Cancer cells are more vulnerable to abnormal redox fluctuations due to their imbalanced antioxidant system, where cell surface receptors sense stress and trigger intracellular signal relay. As canonical targets of many targeted therapies, cell receptors sensitize the cells to specific drugs. On the other hand, cell target mutations are commonly associated with drug resistance. Thus, exploring effective therapeutics targeting diverse cell receptors may open new clinical avenues against aggressive cancers. This paper uses focused case studies to reveal the intrinsic relationship between the cell receptors of different categories and the primary cancer hallmarks that are associated with the responses to external or internal redox perturbations. Cold atmospheric plasma (CAP) is examined as a promising redox modulation medium and highly selective anti-cancer therapeutic modality featuring dynamically varying receptor targets and minimized drug resistance against aggressive cancers.
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Affiliation(s)
- Xiaofeng Dai
- Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China
- Correspondence:
| | - Erik W. Thompson
- School of Biomedical Sciences, Queensland University of Technology (QUT), Brisbane, QLD 4059, Australia
- Translational Research Institute, Brisbane, QLD 4102, Australia
| | - Kostya (Ken) Ostrikov
- School of Chemistry, Physics and Center for Biomedical Technologies, Queensland University of Technology (QUT), Brisbane, QLD 4000, Australia
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Is Silver the New Gold? A Systematic Review of the Preclinical Evidence of Its Use in Bone Substitutes as Antiseptic. Antibiotics (Basel) 2022; 11:antibiotics11080995. [PMID: 35892385 PMCID: PMC9329868 DOI: 10.3390/antibiotics11080995] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/03/2022] [Revised: 07/16/2022] [Accepted: 07/22/2022] [Indexed: 02/01/2023] Open
Abstract
Antibiotic-laden bone substitutes represent a viable option in the treatment of bone and joint infections with bone defects. In particular, the addition of silver ions or silver nanoparticles to bone substitutes to achieve local antiseptic activity could represent a further contribution, also helping to prevent bacterial resistance to antibiotics. An in-depth search of the main scientific databases was performed regarding the use of silver compounds for bone substitution. The available evidence is still limited to the preclinical level: 22 laboratory studies, 2 animal models, and 3 studies, with both in vitro and in vivo analysis, were found on the topic. Numerous biomaterials have been evaluated. In vitro studies confirmed that silver in bone substitutes retains the antibacterial activity already demonstrated in coatings materials. Cytotoxicity was generally found to be low and only related to silver concentrations higher than those sufficient to achieve antibacterial activity. Instead, there are only a few in vivo studies, which appear to confirm antibacterial efficacy, although there is insufficient evidence on the pharmacokinetics and safety profile of the compounds investigated. In conclusion, research on bone substitutes doped with silver is in its early stages, but the preliminary findings seem promising.
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7
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Jagadeeshanayaka N, Awasthi S, Jambagi SC, Srivastava C. Bioactive Surface Modifications through Thermally Sprayed Hydroxyapatite Composite Coatings: A Review over Selective Reinforcements. Biomater Sci 2022; 10:2484-2523. [DOI: 10.1039/d2bm00039c] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
Abstract
Hydroxyapatite (HA) has been an excellent replacement for the natural bone in orthopedic applications, owing to its close resemblance; however, it is brittle and has low strength. Surface modification techniques...
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8
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Yousefpour F, Jamaati R, Aval HJ. Synergistic effects of hybrid (HA+Ag) particles and friction stir processing in the design of a high-strength magnesium matrix bio-nano composite with an appropriate texture for biomedical applications. J Mech Behav Biomed Mater 2021; 125:104983. [PMID: 34823088 DOI: 10.1016/j.jmbbm.2021.104983] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/24/2021] [Revised: 11/16/2021] [Accepted: 11/17/2021] [Indexed: 01/07/2023]
Abstract
In this work, the effects of hybrid (HA + Ag) particles and triple-pass friction stir processing on the microstructure, texture, hardness, and tensile behavior of magnesium matrix bio-nano composite were investigated. The results showed that the mean grain size of samples was in the range of 1-5 μm owing to the occurrence of dynamic recrystallization and suppression of grain growth by second phase particles. All samples exhibited uniform dispersion of particles in the magnesium matrix caused by triple-pass FSP. However, some agglomerations were visible in the microstructure of AZ91/nHA nanocomposite. The average grain size of the AZ91/nHA/smAg sample (1.4 μm) was smaller than that of the AZ91/nHA/mAg sample (2.1 μm), which was attributed to the formation of higher content of MgxAgy precipitates in the AZ91/nHA/smAg composite. By performing the FSP, the content of Mg17Al12 was significantly decreased due to the dissolution of beta into the alpha caused by the breakup effect of mechanical stirring and temperature increase of samples. The AZ91/nHA/smAg sample had the highest texture parameter for the {101‾1} orientation as the high corrosion resistance texture. This was due to the promoting the non-basal slip caused by the dissolution of smAg particles in the magnesium matrix. After the FSP, the microhardness distribution of AZ91, AZ91/nHA, AZ91/nHA/mAg, and AZ91/nHA/smAg samples tended to be uniform and the average hardness was improved owing to the fragmentation of beta particles, grain refinement, and homogeneous dispersion of second phase particles. Compared with the AZ91/nHA/mAg sample, an increase in ultimate tensile strength (291.7 MPa), and a decrease in total elongation (5.6%) and energy absorption (12.3 J/cm3) were observed in the AZ91/nHA/smAg sample due to the formation of a higher content of the silver-rich precipitates in the AZ91/nHA/smAg sample during cooling caused by the higher solubility of silver submicron particles. The fracture surfaces of all processed samples consisted of a large number of fine equiaxed dimples (ductile fracture) owing to the grain refinement and the presence of fine second phase particles.
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Affiliation(s)
- Foroozan Yousefpour
- Department of Materials Engineering, Babol Noshirvani University of Technology, Shariati Ave., Babol, 47148-71167, Iran
| | - Roohollah Jamaati
- Department of Materials Engineering, Babol Noshirvani University of Technology, Shariati Ave., Babol, 47148-71167, Iran.
| | - Hamed Jamshidi Aval
- Department of Materials Engineering, Babol Noshirvani University of Technology, Shariati Ave., Babol, 47148-71167, Iran
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9
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Biocomposites Containing Silver Nanoparticles for Biomedical Applications. J CLUST SCI 2021. [DOI: 10.1007/s10876-021-02180-8] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/20/2022]
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10
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Balu SK, Sampath V, Andra S, Alagar S, Manisha Vidyavathy S. Fabrication of carbon and silver nanomaterials incorporated hydroxyapatite nanocomposites: Enhanced biological and mechanical performances for biomedical applications. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2021; 128:112296. [PMID: 34474847 DOI: 10.1016/j.msec.2021.112296] [Citation(s) in RCA: 11] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/22/2021] [Revised: 06/14/2021] [Accepted: 06/30/2021] [Indexed: 10/20/2022]
Abstract
Hydroxyapatite is widely utilized for different biomedical applications because of its outstanding biocompatibility and bioactivity. Cuttlefish bones, which are available aplenty, are both inexpensive and eco-friendly sources for calcium carbonate. In the present study, cuttlefish bones-derived HAp nanorods have been utilized to fabricate HAp nanocomposites incorporating 1, 3 and 5 wt% each of GO, MWCNTs, GONRs and Ag NPs. Characterization using such techniques as XRD, FTIR, HRSEM and EDS was performed to analyze the physicochemical properties of nanocomposites, and MTT assay, hemolysis, bioactivity and drug release to evaluate the biological properties. The XRD and HRSEM results reveal that crystallite and particle size increase with increasing wt% of carbon nanomaterials and Ag NPs. However, the addition of nanomaterials did not modify the shape of HAp. The MTT assay and hemolysis results suggest GONRs possess better biocompatibility than GO and CNTs due to their smooth edge structure. While adding carbon materials up to 3 wt% caused an increase in the hardness, adding up to 5 wt% of them caused a decrease in the hardness due to the agglomeration of the particles. Biocompatibility and Vicker's hardness studies show that adding carbon nanomaterials up to 3 wt% caused significant improvement in biocompatibility and mechanical properties. Antibacterial activity test was performed to analyze the ability to preclude the formation of biofilms. The results showed better activity for silver-incorporated nanocomposites in the presence of E. coli and S. aureus bacteria. Drug release studies were performed using lidocaine drug and the results showed nearly similar drug release profile for all the samples except HAg3. Finally, nanocomposite HRA3 could be a suitable candidate for biomedical applications since it shows better biological and mechanical properties than GO and MWCNTs nanocomposites.
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Affiliation(s)
- Satheesh Kumar Balu
- Department of Ceramic Technology, Anna University, Chennai, Tamil Nadu 600025, India
| | - V Sampath
- Department of Metallurgical and Materials Engineering, Indian Institute of Technology Madras, Chennai, Tamil Nadu 600036, India
| | - Swetha Andra
- Center for Nanoscience and Technology, Chennai Institute of Technology, Chennai, Tamil Nadu 600069, India
| | - Srinivasan Alagar
- Institute of Nanoscience and Technology, Sector-81, Knowledge City, Sahibzada Ajit Singh Nagar, Punjab 140306, India
| | - S Manisha Vidyavathy
- Department of Ceramic Technology, Anna University, Chennai, Tamil Nadu 600025, India.
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11
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van Hengel IAJ, Tierolf MWAM, Fratila-Apachitei LE, Apachitei I, Zadpoor AA. Antibacterial Titanium Implants Biofunctionalized by Plasma Electrolytic Oxidation with Silver, Zinc, and Copper: A Systematic Review. Int J Mol Sci 2021; 22:3800. [PMID: 33917615 PMCID: PMC8038786 DOI: 10.3390/ijms22073800] [Citation(s) in RCA: 25] [Impact Index Per Article: 6.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/16/2021] [Revised: 04/02/2021] [Accepted: 04/05/2021] [Indexed: 02/06/2023] Open
Abstract
Patients receiving orthopedic implants are at risk of implant-associated infections (IAI). A growing number of antibiotic-resistant bacteria threaten to hamper the treatment of IAI. The focus has, therefore, shifted towards the development of implants with intrinsic antibacterial activity to prevent the occurrence of infection. The use of Ag, Cu, and Zn has gained momentum as these elements display strong antibacterial behavior and target a wide spectrum of bacteria. In order to incorporate these elements into the surface of titanium-based bone implants, plasma electrolytic oxidation (PEO) has been widely investigated as a single-step process that can biofunctionalize these (highly porous) implant surfaces. Here, we present a systematic review of the studies published between 2009 until 2020 on the biomaterial properties, antibacterial behavior, and biocompatibility of titanium implants biofunctionalized by PEO using Ag, Cu, and Zn. We observed that 100% of surfaces bearing Ag (Ag-surfaces), 93% of surfaces bearing Cu (Cu-surfaces), 73% of surfaces bearing Zn (Zn-surfaces), and 100% of surfaces combining Ag, Cu, and Zn resulted in a significant (i.e., >50%) reduction of bacterial load, while 13% of Ag-surfaces, 10% of Cu-surfaces, and none of Zn or combined Ag, Cu, and Zn surfaces reported cytotoxicity against osteoblasts, stem cells, and immune cells. A majority of the studies investigated the antibacterial activity against S. aureus. Important areas for future research include the biofunctionalization of additively manufactured porous implants and surfaces combining Ag, Cu, and Zn. Furthermore, the antibacterial activity of such implants should be determined in assays focused on prevention, rather than the treatment of IAIs. These implants should be tested using appropriate in vivo bone infection models capable of assessing whether titanium implants biofunctionalized by PEO with Ag, Cu, and Zn can contribute to protect patients against IAI.
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Affiliation(s)
- Ingmar A. J. van Hengel
- Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, 2628 CD Delft, The Netherlands; (M.W.A.M.T.); (L.E.F.-A.); (I.A.); (A.A.Z.)
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12
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Verma AS, Sharma A, Kumar A, Mukhopadhyay A, Kumar D, Dubey AK. Multifunctional Response of Piezoelectric Sodium Potassium Niobate (NKN)-Toughened Hydroxyapatite-Based Biocomposites. ACS APPLIED BIO MATERIALS 2020; 3:5287-5299. [DOI: 10.1021/acsabm.0c00642] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/29/2022]
Affiliation(s)
- Alok Singh Verma
- Department of Ceramic Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India
| | - Ankur Sharma
- High Temperature and Energy Materials Laboratory, Department of Metallurgical Engineering and Materials Science, Indian Institute of Technology (IIT) Bombay, Mumbai 400076, India
| | - Ajay Kumar
- High Temperature and Energy Materials Laboratory, Department of Metallurgical Engineering and Materials Science, Indian Institute of Technology (IIT) Bombay, Mumbai 400076, India
| | - Amartya Mukhopadhyay
- High Temperature and Energy Materials Laboratory, Department of Metallurgical Engineering and Materials Science, Indian Institute of Technology (IIT) Bombay, Mumbai 400076, India
| | - Devendra Kumar
- Department of Ceramic Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India
| | - Ashutosh Kumar Dubey
- Department of Ceramic Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India
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13
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Valente KP, Brolo A, Suleman A. From Dermal Patch to Implants-Applications of Biocomposites in Living Tissues. Molecules 2020; 25:E507. [PMID: 31991641 PMCID: PMC7037691 DOI: 10.3390/molecules25030507] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/23/2019] [Revised: 01/19/2020] [Accepted: 01/20/2020] [Indexed: 01/21/2023] Open
Abstract
Composites are composed of two or more materials, displaying enhanced performance and superior mechanical properties when compared to their individual components. The use of biocompatible materials has created a new category of biocomposites. Biocomposites can be applied to living tissues due to low toxicity, biodegradability and high biocompatibility. This review summarizes recent applications of biocomposite materials in the field of biomedical engineering, focusing on four areas-bone regeneration, orthopedic/dental implants, wound healing and tissue engineering.
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Affiliation(s)
| | - Alexandre Brolo
- Department of Chemistry, University of Victoria, Victoria, BC V8P 5C2, Canada;
| | - Afzal Suleman
- Department of Mechanical Engineering, University of Victoria, Victoria, BC V8P 5C2, Canada;
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14
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Pereira KA, Cestari SP, Cucinelli Neto RP, Macedo KR, Mendes LC. Oxidized-sulfonated multi-walled carbon nanotube/hydroxyapatite hybrid particles: Synthesis and characterization. J SOLID STATE CHEM 2019. [DOI: 10.1016/j.jssc.2019.120924] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2022]
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15
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Pandey A, Patel AK, S A, Kumar V, Sharma RK, Kanhed S, Nigam VK, Keshri A, Agarwal A, Balani K. Enhanced Tribological and Bacterial Resistance of Carbon Nanotube with Ceria- and Silver-Incorporated Hydroxyapatite Biocoating. NANOMATERIALS 2018; 8:nano8060363. [PMID: 29794997 PMCID: PMC6027173 DOI: 10.3390/nano8060363] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/30/2018] [Revised: 05/16/2018] [Accepted: 05/21/2018] [Indexed: 11/16/2022]
Abstract
Pertaining to real-life applications (by scaling up) of hydroxyapatite (HA)-based materials, herein is a study illustrating the role of carbon nanotube (CNT) reinforcement with ceria (CeO2) and silver (Ag) in HA on titanium alloy (TiAl6V4) substrate, utilizing the plasma-spraying processing technique, is presented. When compared with pure HA coating enhanced hardness (from 2.5 to 5.8 GPa), elastic modulus (from 110 to 171 GPa), and fracture toughness (from 0.7 to 2.2 MPa·m1/2) elicited a reduced wear rate from 55.3 × 10−5 mm3·N−1·m−1 to 2.1 × 10−5 mm3·N−1·m−1 in HA-CNT-CeO2-Ag. Besides, an order of magnitude lower Archard’s wear constant and a 41% decreased shear stress by for HA-CNT-CeO2-Ag coating depicted the effect of higher hardness and modulus of a material to control its wear phenomenon. Antibacterial property of 46% (bactericidal) is ascribed to Ag in addition to CNT-CeO2 in HA. Nonetheless, the composite coating also portrayed exaggerated L929 fibroblast cell growth (4.8 times more than HA), which was visualized as flat and elongated cells with multiple filopodial protrusions. Hence, synthesis of a material with enhanced mechanical integrity resulting in tribological resistance and cytocompatible efficacy was achieved, thereupon making HA-CNT-CeO2-Ag a scalable potent material for real-life load-bearing implantable bio-coating.
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Affiliation(s)
- Aditi Pandey
- Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur-208016, Uttar Pradesh, India.
| | - Anup Kumar Patel
- Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur-208016, Uttar Pradesh, India.
| | - Ariharan S
- Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur-208016, Uttar Pradesh, India.
| | - Vikram Kumar
- Department of Mechanical Engineering, Indian Institute of Technology Kanpur, Kanpur-208016, Uttar Pradesh, India.
| | - Rajeev Kumar Sharma
- Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur-208016, Uttar Pradesh, India.
| | - Satish Kanhed
- Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur-208016, Uttar Pradesh, India.
| | - Vinod Kumar Nigam
- Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi-835 215, Jharkhand, India.
| | - Anup Keshri
- Department of Materials Science and Engineering, Indian Institute of Technology Patna, Patna-801103, Bihar, India.
| | - Arvind Agarwal
- Department of Mechanical Engineering, Florida International University, Miami, FL 33172, USA.
| | - Kantesh Balani
- Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur-208016, Uttar Pradesh, India.
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Pandey A, Midha S, Sharma RK, Maurya R, Nigam VK, Ghosh S, Balani K. Antioxidant and antibacterial hydroxyapatite-based biocomposite for orthopedic applications. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2018; 88:13-24. [PMID: 29636127 DOI: 10.1016/j.msec.2018.02.014] [Citation(s) in RCA: 50] [Impact Index Per Article: 7.1] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/08/2017] [Revised: 01/14/2018] [Accepted: 02/20/2018] [Indexed: 12/27/2022]
Abstract
Post-implantation, vicinity acquired oxidative stress and bacterial infections lead to apoptosis with eventual bone-resorption and implant failure, respectively. Thus, in order to combat aforementioned complications, present research aims in utilizing antioxidant ceria (CeO2) and antibacterial silver (Ag) reinforced hydroxyapatite (HA) composite with enhanced mechanical and cytocompatible properties. Highly dense (>90%) spark plasma sintered HA-based composites elicits enhanced elastic modulus (121-133 GPa) in comparison to that of HA. The antioxidant activity is quantified using ceria alone, wherein HA-ceria and HA-ceria-Ag pellets exhibits ~36 and 30% antioxidant activity, respectively, accrediting ceria as a scavenger of reactive oxygen species, which was corroborated with the % Ce3+ change quantified by X-ray photoelectron spectroscopy. The HA-Ag pellet shows antibacterial efficacy of ~61% for E. coli and ~53% for S. aureus, while a reduction of ~59% for E. coli and ~50% for S. aureus is observed for HA-ceria-2.5Ag pellet, affirming Ag reinforcement as an established bactericidal agent. The enhanced hydrophobicity on all the HA-based composites affords a high protein adsorption (24 h incubation). Further, elevated hFOB cell count (~6.7 times for HA-ceria-Ag on day 7) with filopodial extensions (60-150 μm) and matrix-like deposition reflect cell-substrate intimacy. Thus, synergistic antioxidant ceria and antibacterial Ag reinforcement with enhanced mechanical integrity can potentially serve as cytocompatible porous bone scaffolds or bioactive coatings on femoral stems.
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Affiliation(s)
- Aditi Pandey
- Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India
| | - Swati Midha
- Department of Textile Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India
| | - Rajeev Kumar Sharma
- Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India
| | - Rita Maurya
- Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India
| | - Vinod Kumar Nigam
- Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835 215, Jharkhand, India
| | - Sourabh Ghosh
- Department of Textile Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
| | - Kantesh Balani
- Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India.
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17
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Tripathi A, Melo JS. Development of Nano-Antimicrobial Biomaterials for Biomedical Applications. ADVANCES IN BIOMATERIALS FOR BIOMEDICAL APPLICATIONS 2017; 66. [PMCID: PMC7122509 DOI: 10.1007/978-981-10-3328-5_12] [Citation(s) in RCA: 21] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Indexed: 12/12/2022]
Abstract
Around the globe, there is a great concern about controlling growth of pathogenic microorganisms for the prevention of infectious diseases. Moreover, the greater incidences of cross contamination and overuse of drugs has contributed towards the development of drug resistant microbial strains making conditions even worse. Hospital acquired infections pose one of the leading complications associated with implantation of any biomaterial after surgery and critical care. In this regard, developing non-conventional antimicrobial agents which would prevent the aforementioned causes is under the quest. The rapid development in nanoscience and nanotechnology has shown promising potential for developing novel biocidal agents that would integrate with a biomaterial to prevent bacterial colonization and biofilm formation. Metals with inherent antimicrobial properties such as silver, copper, zinc at nano scale constitute a special class of antimicrobials which have broad spectrum antimicrobial nature and pose minimum toxicity to humans. Hence, novel biomaterials that inhibit microbial growth would be of great significance to eliminate medical device/instruments associated infections. This chapter comprises the state-of-art advancements in the development of nano-antimicrobial biomaterials for biomedical applications. Several strategies have been targeted to satisfy few important concern such as enhanced long term antimicrobial activity and stability, minimize leaching of antimicrobial material and promote reuse. The proposed strategies to develop new hybrid antimicrobial biomaterials would offer a potent antibacterial solution in healthcare sector such as wound healing applications, tissue scaffolds, medical implants, surgical devices and instruments.
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Affiliation(s)
- Anuj Tripathi
- Nuclear Agriculture & Biotechnology Div, Bhabha Atomic Research Centre, Mumbai, Maharashtra India
| | - Jose Savio Melo
- Nuclear Agriculture & Biotechnology Div, Bhabha Atomic Research Centre, Mumbai, Maharashtra India
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18
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Liu S, Li H, Su Y, Guo Q, Zhang L. Preparation and properties of in-situ growth of carbon nanotubes reinforced hydroxyapatite coating for carbon/carbon composites. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2017; 70:805-811. [DOI: 10.1016/j.msec.2016.09.060] [Citation(s) in RCA: 25] [Impact Index Per Article: 3.1] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/22/2016] [Revised: 08/11/2016] [Accepted: 09/26/2016] [Indexed: 11/24/2022]
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Boda SK, Basu B. Engineered biomaterial and biophysical stimulation as combinatorial strategies to address prosthetic infection by pathogenic bacteria. J Biomed Mater Res B Appl Biomater 2016; 105:2174-2190. [PMID: 27404048 DOI: 10.1002/jbm.b.33740] [Citation(s) in RCA: 8] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/29/2016] [Revised: 06/08/2016] [Accepted: 06/20/2016] [Indexed: 12/25/2022]
Abstract
A plethora of antimicrobial strategies are being developed to address prosthetic infection. The currently available methods for implant infection treatment include the use of antibiotics and revision surgery. Among the bacterial strains, Staphylococcus species pose significant challenges particularly, with regard to hospital acquired infections. In order to combat such life threatening infectious diseases, researchers have developed implantable biomaterials incorporating nanoparticles, antimicrobial reinforcements, surface coatings, slippery/non-adhesive and contact killing surfaces. This review discusses a few of the biomaterial and biophysical antimicrobial strategies, which are in the developmental stage and actively being pursued by several research groups. The clinical efficacy of biophysical stimulation methods such as ultrasound, electric and magnetic field treatments against prosthetic infection depends critically on the stimulation protocol and parameters of the treatment modality. A common thread among the three biophysical stimulation methods is the mechanism of bactericidal action, which is centered on biophysical rupture of bacterial membranes, the generation of reactive oxygen species (ROS) and bacterial membrane depolarization evoked by the interference of essential ion-transport. Although the extent of antimicrobial effect, normally achieved through biophysical stimulation protocol is insufficient to warrant therapeutic application, a combination of antibiotic/ROS inducing agents and biophysical stimulation methods can elicit a clinically relevant reduction in viable bacterial numbers. In this review, we present a detailed account of both the biomaterial and biophysical approaches for achieving maximum bacterial inactivation. Summarizing, the biophysical stimulation methods in a combinatorial manner with material based strategies can be a more potent solution to control bacterial infections. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2174-2190, 2017.
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Affiliation(s)
- Sunil Kumar Boda
- Laboratory for Biomaterials, Materials Research Centre, Indian Institute of Science, Bangalore, 560012, India
| | - Bikramjit Basu
- Laboratory for Biomaterials, Materials Research Centre, Indian Institute of Science, Bangalore, 560012, India.,Centre for Biosystems Science and Engineering, Indian Institute of Science, Bangalore, 560012, India
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20
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Sathishkumar S, Louis K, Shinyjoy E, Gopi D. Tailoring the Sm/Gd-Substituted Hydroxyapatite Coating on Biomedical AISI 316L SS: Exploration of Corrosion Resistance, Protein Profiling, Osteocompatibility, and Osteogenic Differentiation for Orthopedic Implant Applications. Ind Eng Chem Res 2016. [DOI: 10.1021/acs.iecr.5b04329] [Citation(s) in RCA: 39] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/09/2023]
Affiliation(s)
- Saravanan Sathishkumar
- Department
of Chemistry, Periyar University, Salem 636011, India
- Centre
for Nanoscience and Nanotechnology, Periyar University, Salem 636011, India
| | - Kavitha Louis
- Department
of Physics, School of Basic and Applied Sciences, Central University of Tamilnadu, Thiruvarur 610101, India
| | | | - Dhanaraj Gopi
- Department
of Chemistry, Periyar University, Salem 636011, India
- Centre
for Nanoscience and Nanotechnology, Periyar University, Salem 636011, India
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21
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The use of nanomaterials to treat bone infections. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2016; 67:822-833. [PMID: 27287180 DOI: 10.1016/j.msec.2016.04.062] [Citation(s) in RCA: 32] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/21/2015] [Revised: 03/29/2016] [Accepted: 04/18/2016] [Indexed: 12/15/2022]
Abstract
A new era of osteomyelitis treatment has been taking strides towards efficient, local administration of antibiotics at the site of infection. By having them localized to the site of infection, this toxicity is no longer an issue and actually has shown to be a more productive treatment for osteomyelitis. Researchers have focused the production of non-biodegradable, antibiotic, infused bone cements specifically designed for proficient osteocyte binding, useful antibiotic release over a desirable period of time, and promotion of bone regeneration. These cements are then surgically placed on the infected site following debridement and irrigation. The problem, however, is that the use of ineffective cements and the overuse of antibiotics has led to the development of resistant bacteria. Due to this, further research is being done in the field of antibiotic discovery and delivery. Specifically, the development of biodegradable materials capable of efficiently delivering antibiotics and also eliminating the need for follow-up surgery to remove the delivery material is being done, thus reducing exposure risk. Nanoparticles have been developed in the forms of scaffolds and injections to deliver a higher degree and longer lasting duration of antibiotic release, while promoting bone regeneration.
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22
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Groza A, Ciobanu CS, Popa CL, Iconaru SL, Chapon P, Luculescu C, Ganciu M, Predoi D. Structural Properties and Antifungal Activity against Candida albicans Biofilm of Different Composite Layers Based on Ag/Zn Doped Hydroxyapatite-Polydimethylsiloxanes. Polymers (Basel) 2016; 8:E131. [PMID: 30979226 PMCID: PMC6432280 DOI: 10.3390/polym8040131] [Citation(s) in RCA: 28] [Impact Index Per Article: 3.1] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/29/2016] [Revised: 03/25/2016] [Accepted: 03/30/2016] [Indexed: 01/04/2023] Open
Abstract
Modern medicine is still struggling to find new and more effective methods for fighting off viruses, bacteria and fungi. Among the most dangerous and at times life-threatening fungi is Candida albicans. Our work is focused on surface and structural characterization of hydroxyapatite, silver doped hydroxyapatite and zinc doped hydroxyapatite deposited on a titanium substrate previously coated with polydimethylsiloxane (HAp-PDMS, Ag:HAp-PDMS, Zn:HAp-PDMS) by different techniques: Scanning Electron Microscopy (SEM), Glow Discharge Optical Emission Spectroscopy (GDOES) and Fourier Transform Infrared Spectroscopy (FTIR). The morphological studies revealed that the use of the PDMS polymer as an interlayer improves the quality of the coatings. The structural characterizations of the thin films revealed the basic constituents of both apatitic and PDMS structure. In addition, the GD depth profiles indicated the formation of a composite material as well as the successful embedding of the HAp, Zn:HAp and Ag:HAp into the polymer. On the other hand, in vitro evaluation of the antifungal properties of Ag:HAp-PDMS and Zn:HAp-PDMS demonstrated the fungicidal effects of Ag:HAp-PDMS and the potential antifungal effect of Zn:HAp-PDMS composite layers against C. albicans biofilm. The results acquired in this research complete previous research on the potential use of new complex materials produced by nanotechnology in biomedicine.
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Affiliation(s)
- Andreea Groza
- National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor St. P.O. BOX MG 36, Magurele 077125, Romania.
| | - Carmen Steluta Ciobanu
- National Institute for Materials Physics, 405 A Atomistilor Street, P.O. Box MG 07, Magurele 077125, Romania.
| | - Cristina Liana Popa
- National Institute for Materials Physics, 405 A Atomistilor Street, P.O. Box MG 07, Magurele 077125, Romania.
- Faculty of Physics, University of Bucharest, 405 Atomistilor Street, P.O. Box MG1, Magurele 077125, Romania.
| | - Simona Liliana Iconaru
- National Institute for Materials Physics, 405 A Atomistilor Street, P.O. Box MG 07, Magurele 077125, Romania.
| | - Patrick Chapon
- Horiba Jobin Yvon S.A, 16-18, rue du Canal, Longjumeau Cedex 91165, France.
| | - Catalin Luculescu
- National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor St. P.O. BOX MG 36, Magurele 077125, Romania.
| | - Mihai Ganciu
- National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor St. P.O. BOX MG 36, Magurele 077125, Romania.
| | - Daniela Predoi
- National Institute for Materials Physics, 405 A Atomistilor Street, P.O. Box MG 07, Magurele 077125, Romania.
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23
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Galvanic deposition and characterization of brushite/hydroxyapatite coatings on 316L stainless steel. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2016; 64:93-101. [PMID: 27127032 DOI: 10.1016/j.msec.2016.03.088] [Citation(s) in RCA: 21] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/19/2015] [Revised: 03/04/2016] [Accepted: 03/23/2016] [Indexed: 11/21/2022]
Abstract
In this work, brushite and brushite/hydroxyapatite (BS, CaHPO4·H2O; HA, Ca10(PO4)6(OH)2) coatings were deposited on 316L stainless steel (316LSS) from a solution containing Ca(NO3)2·4H2O and NH4H2PO4 by a displacement reaction based on a galvanic contact, where zinc acts as sacrificial anode. Driving force for the cementation reaction arises from the difference in the electrochemical standard potentials of two different metallic materials (316LSS and Zn) immersed in an electrolyte, so forming a galvanic contact leading to the deposition of BS/HA on nobler metal. We found that temperature and deposition time affect coating features (morphology, structure, and composition). Deposits were characterized by means of several techniques. The morphology was investigated by scanning electron microscopy, the elemental composition was obtained by X-ray energy dispersive spectroscopy, whilst the structure was identified by Raman spectroscopy and X-ray diffraction. BS was deposited at all investigated temperatures covering the 316LSS surface. At low and moderate temperature, BS coatings were compact, uniform and with good crystalline degree. On BS layers, HA crystals were obtained at 50°C for all deposition times, while at 25°C, its presence was revealed only after long deposition time. Electrochemical studies show remarkable improvement in corrosion resistance.
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24
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Patel AK, Balani K. Dispersion fraction enhances cellular growth of carbon nanotube and aluminum oxide reinforced ultrahigh molecular weight polyethylene biocomposites. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2015; 46:504-13. [DOI: 10.1016/j.msec.2014.10.075] [Citation(s) in RCA: 21] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/23/2014] [Revised: 10/04/2014] [Accepted: 10/28/2014] [Indexed: 12/01/2022]
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25
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Erakovic S, Jankovic A, Tsui GCP, Tang CY, Miskovic-Stankovic V, Stevanovic T. Novel bioactive antimicrobial lignin containing coatings on titanium obtained by electrophoretic deposition. Int J Mol Sci 2014; 15:12294-322. [PMID: 25019343 PMCID: PMC4139845 DOI: 10.3390/ijms150712294] [Citation(s) in RCA: 50] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/05/2014] [Revised: 05/31/2014] [Accepted: 07/01/2014] [Indexed: 02/07/2023] Open
Abstract
Hydroxyapatite (HAP) is the most suitable biocompatible material for bone implant coatings; its brittleness, however, is a major obstacle, and the reason why research focuses on creating composites with biopolymers. Organosolv lignin (Lig) is used for the production of composite coatings, and these composites were examined in this study. Titanium substrate is a key biomedical material due to its well-known properties, but infections of the implantation site still impose a serious threat. One approach to prevent infection is to improve antimicrobial properties of the coating material. Silver doped hydroxyapatite (Ag/HAP) and HAP coatings on titanium were obtained by an electrophoretic deposition method in order to control deposited coating mass and morphology by varying applied voltage and deposition time. The effect of lignin on microstructure, morphology and thermal behavior of biocomposite coatings was investigated. The results showed that higher lignin concentrations protect the HAP lattice during sintering, improving coating stability. The corrosion stability was evaluated in simulated body fluid (SBF) at 37 °C. Newly formed plate-shaped carbonate-HAP was detected, indicating enhanced bioactive performance. The antimicrobial efficiency of Ag/HAP/Lig was confirmed by its higher reduction of bacteria Staphylococcus aureus TL (S. aureus TL) than of HAP/Lig coating. Cytotoxicity assay revealed that both coatings can be classified as non-toxic against healthy immunocompetent peripheral blood mononuclear cells (PBMC).
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Affiliation(s)
- Sanja Erakovic
- Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade 11000, Serbia.
| | - Ana Jankovic
- Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade 11000, Serbia.
| | - Gary C P Tsui
- Department of Industrial and Systems Engineering, Faculty of Engineering, the Hong Kong Polytechnic University, AG711 Chung Sze Yuen Building, Hung Hom, Kowloon, Hong Kong, China.
| | - Chak-Yin Tang
- Department of Industrial and Systems Engineering, Faculty of Engineering, the Hong Kong Polytechnic University, AG711 Chung Sze Yuen Building, Hung Hom, Kowloon, Hong Kong, China.
| | - Vesna Miskovic-Stankovic
- Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade 11000, Serbia.
| | - Tatjana Stevanovic
- Département des Sciences du Bois et de la Forêt, Faculté de Foresterie, de Géographie et de Géomatique, Université Laval, 2425 rue de la Terrasse, Pavillon Gene-H.-Kruger, Québec G1V 0A6, QC, Canada.
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26
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Domination of volumetric toughening by silver nanoparticles over interfacial strengthening of carbon nanotubes in bactericidal hydroxyapatite biocomposite. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2014; 34:455-67. [DOI: 10.1016/j.msec.2013.09.034] [Citation(s) in RCA: 25] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/13/2013] [Revised: 09/11/2013] [Accepted: 09/28/2013] [Indexed: 02/05/2023]
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Thrivikraman G, Madras G, Basu B. In vitro/In vivo assessment and mechanisms of toxicity of bioceramic materials and its wear particulates. RSC Adv 2014; 4:12763. [DOI: 10.1039/c3ra44483j] [Citation(s) in RCA: 49] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/03/2025] Open
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28
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Bajpai I, Balani K, Basu B. Synergistic effect of static magnetic field and HA-Fe3O4 magnetic composites on viability of S. aureus and E. coli bacteria. J Biomed Mater Res B Appl Biomater 2013; 102:524-32. [PMID: 24142888 DOI: 10.1002/jbm.b.33031] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/05/2013] [Revised: 08/10/2013] [Accepted: 08/18/2013] [Indexed: 11/08/2022]
Abstract
In addressing the issue of prosthetic infection, this work demonstrated the synergistic effect of the application of static magnetic field (SMF) and ferrimagnetic substrate properties on the bactericidal property in vitro. This aspect was studied using hydroxyapatite (HA)-xFe3 O4 (x=10, 20, and 40 wt.%) substrates, which have different saturation magnetization properties. During bacteria culture experiments, 100 mT SMF was applied to growth medium (with HA-xFe3 O4 substrate) in vitro for 30, 120, and 240 min. A combination of MTT assay, membrane rupture assays, live/dead assay, and fluorescence microscopic analysis showed that the bactericidal effect of SMF increases with the exposure duration as well as increasing Fe3 O4 content in biomaterial substrates. Importantly, the synergistic bactericidal effect was found to be independent of bacterial cell type, as similar qualitative trend is measured with both gram negative Escherichia coli (E. coli) and gram positive Staphylococcus aureus (S. aureus) strains. The reduction in E. coli viability was 83% higher on HA-40 Wt % Fe3 O4 composite after 4 h exposure to SMF as compared to nonexposed control. Interestingly, any statistically significant difference in ROS was not observed in bacterial growth medium after magnetic field exposure, indicating the absence of ROS enhancement due to magnetic field. Overall, this study illustrates significant role being played by magnetic substrate compositions towards bactericidal property than by magnetic field exposure alone.
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Affiliation(s)
- Indu Bajpai
- Department of Materials Science and Engineering, Laboratory for Biomaterials, Indian Institute of Technology, Kanpur, 208016, Uttar Pradesh, India
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Mututuvari TM, Harkins AL, Tran CD. Facile synthesis, characterization, and antimicrobial activity of cellulose-chitosan-hydroxyapatite composite material: a potential material for bone tissue engineering. J Biomed Mater Res A 2013; 101:3266-77. [PMID: 23595871 DOI: 10.1002/jbm.a.34636] [Citation(s) in RCA: 16] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/04/2012] [Revised: 01/18/2013] [Accepted: 02/04/2013] [Indexed: 11/09/2022]
Abstract
Hydroxyapatite (HAp) is often used as a bone-implant material because it is biocompatible and osteoconductive. However, HAp possesses poor rheological properties and it is inactive against disease-causing microbes. To improve these properties, we developed a green method to synthesize multifunctional composites containing: (1) cellulose (CEL) to impart mechanical strength; (2) chitosan (CS) to induce antibacterial activity thereby maintaining a microbe-free wound site; and (3) HAp. In this method, CS and CEL were co-dissolved in an ionic liquid (IL) and then regenerated from water. HAp was subsequently formed in situ by alternately soaking [CEL+CS] composites in aqueous solutions of CaCl2 and Na2 HPO4 . At least 88% of IL used was recovered for reuse by distilling the aqueous washings of [CEL+CS]. The composites were characterized using FTIR, XRD, and SEM. These composites retained the desirable properties of their constituents. For example, the tensile strength of the composites was enhanced 1.9 times by increasing CEL loading from 20% to 80%. Incorporating CS in the composites resulted in composites which inhibited the growth of both Gram positive (MRSA, S. aureus and VRE) and Gram negative (E. coli and P. aeruginosa) bacteria. These findings highlight the potential use of [CEL+CS+HAp] composites as scaffolds in bone tissue engineering.
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Affiliation(s)
- Tamutsiwa M Mututuvari
- Department of Chemistry, Marquette University, P. O. Box 1881, Milwaukee, Wisconsin, 53201
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