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Nogueira DMB, Rosso MPDO, Santos PSDS, Sousa-Neto MD, Silva-Sousa AC, Soares CT, Reis CHB, Rossi JDO, Bueno CRDS, Buchaim DV, Buchaim RL, Zangrando MSR. Biological Behavior of Bioactive Glasses SinGlass (45S5) and SinGlass High (F18) in the Repair of Critical Bone Defects. Biomolecules 2025; 15:112. [PMID: 39858506 PMCID: PMC11763790 DOI: 10.3390/biom15010112] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/12/2024] [Revised: 01/06/2025] [Accepted: 01/08/2025] [Indexed: 01/27/2025] Open
Abstract
This study evaluated the osteogenic potential of the bioactive glasses SinGlass (45S5) and SinGlass High (F18) in regenerating critical bone defects in rat calvaria. Both biomaterials promoted new bone formation around the particles, with the SinGlass High (F18) group exhibiting a higher rate of bone maturation. Histomorphological and birefringence analyses revealed better organization of the newly formed bone in the biomaterial-treated groups, and immunohistochemistry indicated the expression of osteogenic markers such as osteocalcin, immunostaining for bone morphogenetic protein 2 (BMP 2), and immunostaining for bone morphogenetic protein 4 (BMP 4). Microtomography computadorized (Micro-CT) revealed centripetal bone formation in both groups, with greater integration of the particles into the surrounding bone tissue. The superior performance of SinGlass High (F18) was attributed to its higher potassium and magnesium content, which enhance osteoconductivity. After 42 days, the SinGlass High (F18) group showed the highest percentage of new bone formation, in line with previous studies. Although our results are promising, the limited follow-up period and use of a single animal model highlight the need for further research to validate clinical applicability. SinGlass High (F18) appears to be a viable alternative to autografts in bone repair, with potential to improve tissue integration and accelerate recovery.
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Affiliation(s)
- Dayane Maria Braz Nogueira
- Department of Anatomy, Faculty of Higher Education of the Interior of São Paulo (FAIP), Marília 17512-130, Brazil;
| | | | - Paulo Sérgio da Silva Santos
- Department of Surgery, Stomatology, Pathology, and Radiology, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, Brazil;
| | - Manoel Damião Sousa-Neto
- Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, Brazil; (M.D.S.-N.); (A.C.S.-S.)
| | - Alice Corrêa Silva-Sousa
- Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, Brazil; (M.D.S.-N.); (A.C.S.-S.)
| | - Cleverson Teixeira Soares
- Lauro de Souza Lima Institute (ILSL), Anatomical Pathology Laboratory of Bauru (ANATOMED), Bauru 17034-971, Brazil;
| | | | | | | | - Daniela Vieira Buchaim
- Anatomy Department, Medical School, University Center of Adamantina (FAI), Adamantina 17800-000, Brazil;
- Graduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), Sao Paulo 05508-270, Brazil
| | - Rogério Leone Buchaim
- Graduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), Sao Paulo 05508-270, Brazil
- Department of Biological Sciences, Bauru School of Dentistry (FOB/USP), University of São Paulo, Bauru 17012-901, Brazil
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Özkabadayı Y, Türk M, Kumandaş A, Karahan S. Amino acid surface modified bioglass: A candidate biomaterial for bone tissue engineering 1. Microsc Res Tech 2025; 88:26-41. [PMID: 39154380 PMCID: PMC11652810 DOI: 10.1002/jemt.24659] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/31/2023] [Revised: 07/08/2024] [Accepted: 07/09/2024] [Indexed: 08/20/2024]
Abstract
Bioglasses are solid materials consisted of sodium oxide, calcium oxide, silicon dioxide and phosphorus in various proportions and have used in bone tissue engineering. There have been ongoing efforts to improve the surface properties of bioglasses to increase biocompatibility and performance. The aim of the present study is to modify the bioglass surface with an amino acid mixture consisting of arginine, aspartic acid, phenylalanine, cysteine, histidine and lysine, to characterize the surface, and to evaluate the performance and biocompatibility in vitro and in vivo. The untreated bioglass, bioglass kept in simulated body fluid (SBF), and modified bioglass were used in further evaluation. After confirmation of the surface modification with FT-IR analyses and SEM analyses, MC3T3-E1 preosteoblasts adhesion on the surface was also revealed by SEM. The modified bioglass had significantly higher ALP activity in colorimetric measurement, rate of calcium accumulations in Alizarin red s staining, lower rate of cell death in Annexin-V/PI staining to determine apoptosis and necrosis. Having higher cell viability rate in MTT test and absence of genotoxicity in micronucleus test (OECD 487), the modified bioglass was further confirmed for biocompatibility in vitro. The results of the rat tibial defect model revealed that the all bioglass treatments had a significantly better bone healing score compared to the untreated negative control. However, the modified bioglass exhibited significantly better bone healing efforts especially during the first and the second months compared to the other bioglass treatment treatments. As a result, the amino acid surface modification of bioglasses improves the surface biocompatibility and osteogenic performance that makes the amino acid modified bioglass a better candidate for bone tissue engineering. RESEARCH HIGHLIGHTS: Bioglass surface modification with amino acids contributes to bioglass-tissue interaction with an improved cell attachment. Modified bioglass increases in vitro Alp activity and calcium accumulation, and also positively affects cell behavior by supporting cell adaptation. Bioglass exerts osteogenic potential in vivo especially during early bone healing.
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Affiliation(s)
- Yasin Özkabadayı
- Faculty of Veterinary Medicine, Department of Histology and EmbryologyKirikkale UniversityKirikkaleTurkey
| | - Mustafa Türk
- Faculty of Engineering and Natural Sciences, Department of BioengineeringKirikkale UniversityKirikkaleTurkey
| | - Ali Kumandaş
- Faculty of Veterinary Medicine, Department of SurgeryKirikkale UniversityKirikkaleTurkey
- Faculty of Veterinary MedicineKyrgyz‐Turkish Manas UniversityBishkekKyrgyzstan
| | - Siyami Karahan
- Faculty of Veterinary Medicine, Department of Histology and EmbryologyKirikkale UniversityKirikkaleTurkey
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Rajendran AK, Anthraper MSJ, Hwang NS, Rangasamy J. Osteogenesis and angiogenesis promoting bioactive ceramics. MATERIALS SCIENCE AND ENGINEERING: R: REPORTS 2024; 159:100801. [DOI: 10.1016/j.mser.2024.100801] [Citation(s) in RCA: 1] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/06/2025]
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Nogueira DMB, Rosso MPDO, Buchaim DV, Zangrando MSR, Buchaim RL. Update on the use of 45S5 bioactive glass in the treatment of bone defects in regenerative medicine. World J Orthop 2024; 15:204-214. [PMID: 38596193 PMCID: PMC10999964 DOI: 10.5312/wjo.v15.i3.204] [Citation(s) in RCA: 4] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/16/2023] [Revised: 01/15/2024] [Accepted: 02/01/2024] [Indexed: 03/15/2024] Open
Abstract
Bone regeneration is a critical area in regenerative medicine, particularly in orthopedics, demanding effective biomedical materials for treating bone defects. 45S5 bioactive glass (45S5 BG) is a promising material because of its osteoconductive and bioactive properties. As research in this field continues to advance, keeping up-to-date on the latest and most successful applications of this material is imperative. To achieve this, we conducted a comprehensive search on PubMed/MEDLINE, focusing on English articles published in the last decade. Our search used the keywords "bioglass 45S5 AND bone defect" in combination. We found 27 articles, and after applying the inclusion criteria, we selected 15 studies for detailed examination. Most of these studies compared 45S5 BG with other cement or scaffold materials. These comparisons demonstrate that the addition of various composites enhances cellular biocompatibility, as evidenced by the cells and their osteogenic potential. Moreover, the use of 45S5 BG is enhanced by its antimicrobial properties, opening avenues for additional investigations and applications of this biomaterial.
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Affiliation(s)
- Dayane Maria Braz Nogueira
- Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, Brazil
| | | | - Daniela Vieira Buchaim
- Medical School, University Center of Adamantina, Adamantina 17800-000, Brazil
- Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marília, Marília 17525-902, Brazil
| | | | - Rogério Leone Buchaim
- Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, Brazil
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Ferreira FV, Souza AG, Ajdary R, de Souza LP, Lopes JH, Correa DS, Siqueira G, Barud HS, Rosa DDS, Mattoso LH, Rojas OJ. Nanocellulose-based porous materials: Regulation and pathway to commercialization in regenerative medicine. Bioact Mater 2023; 29:151-176. [PMID: 37502678 PMCID: PMC10368849 DOI: 10.1016/j.bioactmat.2023.06.020] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/04/2023] [Revised: 06/16/2023] [Accepted: 06/24/2023] [Indexed: 07/29/2023] Open
Abstract
We review the recent progress that have led to the development of porous materials based on cellulose nanostructures found in plants and other resources. In light of the properties that emerge from the chemistry, shape and structural control, we discuss some of the most promising uses of a plant-based material, nanocellulose, in regenerative medicine. Following a brief discussion about the fundamental aspects of self-assembly of nanocellulose precursors, we review the key strategies needed for material synthesis and to adjust the architecture of the materials (using three-dimensional printing, freeze-casted porous materials, and electrospinning) according to their uses in tissue engineering, artificial organs, controlled drug delivery and wound healing systems, among others. For this purpose, we map the structure-property-function relationships of nanocellulose-based porous materials and examine the course of actions that are required to translate innovation from the laboratory to industry. Such efforts require attention to regulatory aspects and market pull. Finally, the key challenges and opportunities in this nascent field are critically reviewed.
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Affiliation(s)
- Filipe V. Ferreira
- Nanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentation – Rua XV de Novembro, 1452, São Carlos, SP, 13560-979, Brazil
| | - Alana G. Souza
- Center for Engineering, Modeling, and Applied Social Sciences (CECS), Federal University of ABC (UFABC), Santo André, Brazil
| | - Rubina Ajdary
- Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, P. O. Box 16300, Aalto, Espoo, FIN-00076, Finland
| | - Lucas P. de Souza
- College of Engineering and Physical Sciences, Aston Institute of Materials Research, Aston University, Birmingham, UK
| | - João H. Lopes
- Department of Chemistry, Division of Fundamental Sciences (IEF), Technological Institute of Aeronautics (ITA), São Jose dos Campos, SP, Brazil
| | - Daniel S. Correa
- Nanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentation – Rua XV de Novembro, 1452, São Carlos, SP, 13560-979, Brazil
| | - Gilberto Siqueira
- Laboratory for Cellulose & Wood Materials, Empa - Swiss Federal Laboratories for Materials Science and Technology, Überlandstrasse 129, 8600, Dübendorf, Switzerland
| | - Hernane S. Barud
- Biopolymers and Biomaterials Laboratory (BIOPOLMAT), University of Araraquara (UNIARA), Araraquara, 14801-340, São Paulo, Brazil
| | - Derval dos S. Rosa
- Center for Engineering, Modeling, and Applied Social Sciences (CECS), Federal University of ABC (UFABC), Santo André, Brazil
| | - Luiz H.C. Mattoso
- Nanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentation – Rua XV de Novembro, 1452, São Carlos, SP, 13560-979, Brazil
| | - Orlando J. Rojas
- Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, P. O. Box 16300, Aalto, Espoo, FIN-00076, Finland
- Bioproducts Institute, Department of Chemical & Biological Engineering, Department of Chemistry and, Department of Wood Science, The University of British Columbia, 2360 East Mall, Vancouver, BC, V6T 1Z3, Canada
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Sivakumar PM, Yetisgin AA, Demir E, Sahin SB, Cetinel S. Polysaccharide-bioceramic composites for bone tissue engineering: A review. Int J Biol Macromol 2023; 250:126237. [PMID: 37567538 DOI: 10.1016/j.ijbiomac.2023.126237] [Citation(s) in RCA: 13] [Impact Index Per Article: 6.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/05/2023] [Revised: 07/05/2023] [Accepted: 08/07/2023] [Indexed: 08/13/2023]
Abstract
Limitations associated with conventional bone substitutes such as autografts, increasing demand for bone grafts, and growing elderly population worldwide necessitate development of unique materials as bone graft substitutes. Bone tissue engineering (BTE) would ensure therapy advancement, efficiency, and cost-effective treatment modalities of bone defects. One way of engineering bone tissue scaffolds by mimicking natural bone tissue composed of organic and inorganic phases is to utilize polysaccharide-bioceramic hybrid composites. Polysaccharides are abundant in nature, and present in human body. Biominerals, like hydroxyapatite are present in natural bone and some of them possess osteoconductive and osteoinductive properties. Ion doped bioceramics could substitute protein-based biosignal molecules to achieve osteogenesis, vasculogenesis, angiogenesis, and stress shielding. This review is a systemic summary on properties, advantages, and limitations of polysaccharide-bioceramic/ion doped bioceramic composites along with their recent advancements in BTE.
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Affiliation(s)
- Ponnurengam Malliappan Sivakumar
- Nanotechnology Research and Application Center (SUNUM), Sabanci University, Istanbul 34956, Turkey; Institute of Research and Development, Duy Tan University, Da Nang 550000, Viet Nam; School of Medicine and Pharmacy, Duy Tan University, Da Nang 550000, Viet Nam.
| | - Abuzer Alp Yetisgin
- Nanotechnology Research and Application Center (SUNUM), Sabanci University, Istanbul 34956, Turkey; Sabanci University, Faculty of Engineering and Natural Sciences, Materials Science and Nano-Engineering Program, Istanbul 34956, Turkey
| | - Ebru Demir
- Nanotechnology Research and Application Center (SUNUM), Sabanci University, Istanbul 34956, Turkey; Sabanci University, Faculty of Engineering and Natural Sciences, Molecular Biology, Genetics and Bioengineering Program, Istanbul 34956, Turkey
| | - Sevilay Burcu Sahin
- Nanotechnology Research and Application Center (SUNUM), Sabanci University, Istanbul 34956, Turkey; Sabanci University, Faculty of Engineering and Natural Sciences, Molecular Biology, Genetics and Bioengineering Program, Istanbul 34956, Turkey
| | - Sibel Cetinel
- Nanotechnology Research and Application Center (SUNUM), Sabanci University, Istanbul 34956, Turkey; Sabanci University, Faculty of Engineering and Natural Sciences, Molecular Biology, Genetics and Bioengineering Program, Istanbul 34956, Turkey.
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7
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Cell–scaffold interactions in tissue engineering for oral and craniofacial reconstruction. Bioact Mater 2023; 23:16-44. [DOI: 10.1016/j.bioactmat.2022.10.029] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/31/2022] [Revised: 10/22/2022] [Accepted: 10/30/2022] [Indexed: 11/09/2022] Open
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Li S, Cui Y, Liu H, Tian Y, Wang G, Fan Y, Wang J, Wu D, Wang Y. Application of bioactive metal ions in the treatment of bone defects. J Mater Chem B 2022; 10:9369-9388. [PMID: 36378123 DOI: 10.1039/d2tb01684b] [Citation(s) in RCA: 32] [Impact Index Per Article: 10.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/16/2022]
Abstract
The treatment of bone defects is an important problem in clinical practice. The rapid development of bone tissue engineering (BTE) may provide a new method for bone defect treatment. Metal ions have been widely studied in BTE and demonstrated a significant effect in promoting bone tissue growth. Different metal ions can be used to treat bone defects according to specific conditions, including promoting osteogenic activity, inhibiting osteoclast activity, promoting vascular growth, and exerting certain antibacterial effects. Multiple studies have confirmed that metal ions-modified composite scaffolds can effectively promote bone defect healing. By studying current extensive research on metal ions in the treatment of bone defects, this paper reviews the mechanism of metal ions in promoting bone tissue growth, analyzes the loading mode of metal ions, and lists some specific applications of metal ions in different types of bone defects. Finally, this paper summarizes the advantages and disadvantages of metal ions and analyzes the future research trend of metal ions in BTE. This article can provide some new strategies and methods for future research and applications of metal ions in the treatment of bone defects.
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Affiliation(s)
- Shaorong Li
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, P. R. China.
| | - Yutao Cui
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, P. R. China.
| | - He Liu
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, P. R. China.
| | - Yuhang Tian
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, P. R. China.
| | - Gan Wang
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, P. R. China.
| | - Yi Fan
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, P. R. China.
| | - Jingwei Wang
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, P. R. China.
| | - Dankai Wu
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, P. R. China.
| | - Yanbing Wang
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, P. R. China.
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9
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Souza L, Ferreira FV, Lopes JH, Camilli JA, Martin RA. Cancer Inhibition and In Vivo Osteointegration and Compatibility of Gallium-Doped Bioactive Glasses for Osteosarcoma Applications. ACS APPLIED MATERIALS & INTERFACES 2022; 14:45156-45166. [PMID: 36170227 PMCID: PMC9562271 DOI: 10.1021/acsami.2c12102] [Citation(s) in RCA: 16] [Impact Index Per Article: 5.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/08/2023]
Abstract
Traditional osteosarcoma therapies tend to focus solely on eradicating residual cancer cells and often fail to promote local bone regeneration and even inhibit it due to lack of precise control over target cells, i.e., the treatment affects both normal and cancer cells. Typically, multistep procedures are required for optimal efficacy. Here, we found that a silica-based bioactive material containing 3 mol % gallium oxide selectively kills human osteosarcoma cells and presents excellent in vivo osteointegration, while showing no local or systemic toxicity. Cell culture media conditioned with the proposed material was able to kill 41% of osteosarcoma cells, and no significant deleterious effect on normal human osteoblasts was observed. In addition, rats treated with the gallium-doped material showed excellent material-bone integration with no sign of local toxicity or implant rejection. Systemic biocompatibility investigation did not indicate any sign of toxicity, with no presence of fibrosis or cellular infiltrate in the histological microstructure of the liver and kidneys after 56 days of observation. Taken together, these results show that synergistic bone regeneration and targeted cancer therapy can be combined, paving the way toward new bone cancer treatment approaches.
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Affiliation(s)
- Lucas Souza
- Engineering
for Heath Research Centre, College of Engineering & Physical Sciences, Aston University, Birmingham B4 7ET, United Kingdom
| | - Filipe V. Ferreira
- Embrapa
Instrumentation, Nanotechnology National Laboratory for Agriculture, XV de Novembro, 1452, Sao Carlos 13560-970, Brazil
| | - Joao H. Lopes
- Department
of Chemistry, Aeronautics Institute of Technology, Praça Marechal Eduardo Gomes
50, Vila das Acacias, São José dos Campos, São Paulo 12228-900, Brazil
| | - Jose Angelo Camilli
- Department
of Functional and Structural Biology, State
University of Campinas, Campinas13083-970, Sao Paulo, Brazil
| | - Richard A. Martin
- Engineering
for Heath Research Centre, College of Engineering & Physical Sciences, Aston University, Birmingham B4 7ET, United Kingdom
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Zhao F, Yang Z, Xiong H, Yan Y, Chen X, Shao L. A bioactive glass functional hydrogel enhances bone augmentation via synergistic angiogenesis, self-swelling and osteogenesis. Bioact Mater 2022; 22:201-210. [PMID: 36246665 PMCID: PMC9535384 DOI: 10.1016/j.bioactmat.2022.09.007] [Citation(s) in RCA: 18] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/25/2022] [Revised: 08/28/2022] [Accepted: 09/12/2022] [Indexed: 12/03/2022] Open
Abstract
Bone augmentation materials usually cannot provide enough new bone for dental implants due to the material degradation and mucosal pressure. The use of hydrogels with self-swelling properties may provide a higher bone augmentation, although swelling is generally considered to be a disadvantage in tissue engineering. Herein, a double-crosslinked gelatin-hyaluronic acid hydrogels (GH) with self-swelling properties were utilized. Meanwhile, niobium doped bioactive glasses (NbBG) was dispersed in the hydrogel network to prepare the GH-NbBG hydrogel. The composite hydrogel exhibited excellent biocompatibility and the addition of NbBG significantly improved the mechanical properties of the hydrogel. In vivo results found that GH-NbBG synergistically promoted angiogenesis and increased bone augmentation by self-swelling at the early stage of implantation. In addition, at the late stage after implantation, GH-NbBG significantly promoted new bone formation by activating RUNX2/Bglap signaling pathway. Therefore, this study reverses the self-swelling disadvantage of hydrogels into advantage and provides novel ideas for the application of hydrogels in bone augmentation.
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Affiliation(s)
- Fujian Zhao
- Stomatological Hospital, Southern Medical University, Guangzhou, 510280, China
| | - Zhen Yang
- Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China,National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, China
| | - Huacui Xiong
- Stomatological Hospital, Southern Medical University, Guangzhou, 510280, China
| | - Yang Yan
- Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China,National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, China
| | - Xiaofeng Chen
- Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China,National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, China,Corresponding author. Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
| | - Longquan Shao
- Stomatological Hospital, Southern Medical University, Guangzhou, 510280, China,Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Guangzhou, 510515, China,Corresponding author. Stomatological Hospital, Southern Medical University, Guangzhou, 510280, China.
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11
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Khare D, Majumdar S, Krishnamurthy S, Dubey AK. An in vivo toxicity assessment of piezoelectric sodium potassium niobate [Na xK 1-xNbO 3 (x = 0.2-0.8)] nanoparticulates towards bone tissue engineering approach. BIOMATERIALS ADVANCES 2022; 140:213080. [PMID: 35985067 DOI: 10.1016/j.bioadv.2022.213080] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/22/2022] [Revised: 07/26/2022] [Accepted: 08/08/2022] [Indexed: 06/15/2023]
Abstract
One of the recent challenges in the design/development of prosthetic orthopedic implants is to address the concern of local/systemic toxicity of debris particles, released due to wear or degradation. Such debris particles often lead to inflammation at the implanted site or aseptic loosening of the prosthesis which results in failure of the implant during long run. Several in vitro studies demonstrated the potentiality of piezoelectric sodium potassium niobate [NaxK1-xNbO3 (x = 0.2, 0.5, 0.8), NKN] as an emerging next-generation polarizable orthopedic implant. In this perspective, we performed an in vivo study to examine the local and systemic toxicity of NKN nanoparticulates, as a first report. In the present study, male Wistar rats were intra-articularly injected to the knee joint with 100 μl of NKN nanoparticulates (25 mg/ml in normal saline). After 7 days of exposure, the histopathological analyses demonstrate the absence of any inflammation or dissemination of nanoparticulates in vital organs such as heart, liver, kidney and spleen. The anti-inflammatory cytokines (IL-4 and IL-10) profile analyses suggest the increased anti-inflammatory response in the treated rats as compared to non-injected (control) rats, preferably for the sodium and potassium rich NKN i.e., Na0.8K0.2NbO3 and Na0.2K0.8NbO3. The biochemical analyses revealed no pathological changes in the liver and kidney of particulate treated rats. The present study is the first proof to confirm the non-toxic nature of NKN nanoparticulates which provides a step forward towards the development of prosthetic orthopedic implants using biocompatible piezoelectric NKN ceramics.
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Affiliation(s)
- Deepak Khare
- Department of Ceramic Engineering, Indian Institute of Technology (BHU) Varanasi, 221005, India
| | - Shreyasi Majumdar
- Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU) Varanasi, 221005, India
| | - Sairam Krishnamurthy
- Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU) Varanasi, 221005, India
| | - Ashutosh Kumar Dubey
- Department of Ceramic Engineering, Indian Institute of Technology (BHU) Varanasi, 221005, India.
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Khare D, Singh P, Dubey AK. Interplay of surface polarization charge, dynamic electrical stimulation and compositional modification towards accelerated osteogenic response of Na xK 1-xNbO 3 piezo-bioceramics. BIOMATERIALS ADVANCES 2022; 140:213042. [PMID: 35914328 DOI: 10.1016/j.bioadv.2022.213042] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/24/2022] [Revised: 07/10/2022] [Accepted: 07/19/2022] [Indexed: 06/15/2023]
Abstract
Bone remodeling processes involve endogenous bioelectrical signals such as piezoelectric charges. Moreover, external electrical stimulation helps in improving the healing capability of injured tissues by modulating the metabolic signaling pathways of cells. Towards this end, the present study reveals the influence of the combined action of electrostatic surface polarization charge and dynamic pulsed electrical stimulation alongwith compositional modification towards improving the osteogenic response of emerging piezo-bioceramics, sodium potassium niobate [NaxK1-xNbO3 (x = 0.2-0.8), NKN]. The dependence of crystal structure on compositions (x) was retrieved by Rietveld refinement and X-ray peak profile analyses. The surface charge, stored in the polarized (@ 25 kV at 500 °C) NaxK1-xNbO3 (x = 0.2, 0.5, 0.8) samples were measured to be 0.52, 0.50 and 0.47 μC/cm2, respectively, using thermally stimulated depolarized current (TSDC). X-ray photoelectron spectroscopy (XPS) survey scan spectra revealed that the polarization process does not alter the surface chemistry of NKN. Negatively charged surfaces are observed to accelerate early-stage adhesion of osteoblast-like cells which further results in enhanced spreading of adhered cells. Subsequently, the dynamic pulsed electrical stimulation of 1 V/cm with the pulse duration of 400 μs was applied, while the cells were being adhered on electrostatically charged surfaces. The quantitative and qualitative analyses revealed that the synergistic action of electrostatic surface polarization charge and dynamic pulsed electrical stimulation further accelerates cell proliferation and differentiation on negatively charged surfaces of Na and K-rich compositions of NKN. The mechanism of augmented cellular activity was analyzed using intracellular Ca2+ measurement.
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Affiliation(s)
- Deepak Khare
- Department of Ceramic Engineering, Indian Institute of Technology (BHU), Varanasi 221005, INDIA
| | - Priya Singh
- Department of Ceramic Engineering, Indian Institute of Technology (BHU), Varanasi 221005, INDIA
| | - Ashutosh Kumar Dubey
- Department of Ceramic Engineering, Indian Institute of Technology (BHU), Varanasi 221005, INDIA.
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Pantulap U, Arango-Ospina M, Boccaccini AR. Bioactive glasses incorporating less-common ions to improve biological and physical properties. JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE 2021; 33:3. [PMID: 34940923 PMCID: PMC8702415 DOI: 10.1007/s10856-021-06626-3] [Citation(s) in RCA: 53] [Impact Index Per Article: 13.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/19/2021] [Accepted: 11/07/2021] [Indexed: 05/29/2023]
Abstract
Bioactive glasses (BGs) have been a focus of research for over five decades for several biomedical applications. Although their use in bone substitution and bone tissue regeneration has gained important attention, recent developments have also seen the expansion of BG applications to the field of soft tissue engineering. Hard and soft tissue repair therapies can benefit from the biological activity of metallic ions released from BGs. These metallic ions are incorporated in the BG network not only for their biological therapeutic effects but also in many cases for influencing the structure and processability of the glass and to impart extra functional properties. The "classical" elements in silicate BG compositions are silicon (Si), phosphorous (P), calcium (Ca), sodium (Na), and potassium (K). In addition, other well-recognized biologically active ions have been incorporated in BGs to provide osteogenic, angiogenic, anti-inflammatory, and antibacterial effects such as zinc (Zn), magnesium (Mg), silver (Ag), strontium (Sr), gallium (Ga), fluorine (F), iron (Fe), cobalt (Co), boron (B), lithium (Li), titanium (Ti), and copper (Cu). More recently, rare earth and other elements considered less common or, some of them, even "exotic" for biomedical applications, have found room as doping elements in BGs to enhance their biological and physical properties. For example, barium (Ba), bismuth (Bi), chlorine (Cl), chromium (Cr), dysprosium (Dy), europium (Eu), gadolinium (Gd), ytterbium (Yb), thulium (Tm), germanium (Ge), gold (Au), holmium (Ho), iodine (I), lanthanum (La), manganese (Mn), molybdenum (Mo), nickel (Ni), niobium (Nb), nitrogen (N), palladium (Pd), rubidium (Rb), samarium (Sm), selenium (Se), tantalum (Ta), tellurium (Te), terbium (Tb), erbium (Er), tin (Sn), tungsten (W), vanadium (V), yttrium (Y) as well as zirconium (Zr) have been included in BGs. These ions have been found to be particularly interesting for enhancing the biological performance of doped BGs in novel compositions for tissue repair (both hard and soft tissue) and for providing, in some cases, extra functionalities to the BG, for example fluorescence, luminescence, radiation shielding, anti-inflammatory, and antibacterial properties. This review summarizes the influence of incorporating such less-common elements in BGs with focus on tissue engineering applications, usually exploiting the bioactivity of the BG in combination with other functional properties imparted by the presence of the added elements.
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Affiliation(s)
- Usanee Pantulap
- Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, 91058, Erlangen, Germany
| | - Marcela Arango-Ospina
- Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, 91058, Erlangen, Germany
| | - Aldo R Boccaccini
- Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, 91058, Erlangen, Germany.
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14
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The Effects of Graphene on the Biocompatibility of a 3D-Printed Porous Titanium Alloy. COATINGS 2021. [DOI: 10.3390/coatings11121509] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/14/2022]
Abstract
3D-printed titanium (Ti) materials have attracted much attention in the field of bone tissue repair. However, the combination strength of traditional alloy materials with bone tissue is lower, and the elastic modulus is higher than that of natural bone tissue, which makes the titanium alloy susceptible to stress shielding phenomena after implantation. Therefore, it is urgent to find better surface modification technology. In this study, the physical and chemical properties, toxicity, and proliferation of adipose stem cells of composite graphene-coated titanium alloy (Gr–Ti) were investigated using 3D-printed titanium alloy as a material model. Physical and chemical property tests confirmed that 3D printing could produce porous titanium alloy materials; the compressive strength and elastic modulus of the titanium alloy scaffolds were 91 ± 3 MPa and 3.1 ± 0.4 GPa, matching the elastic modulus of normal bone tissue. The surface characterization shows that graphene can be coated on titanium alloy by a micro-arc oxidation process, which significantly improves the surface roughness of titanium alloy. The roughness factor (Ra) of the Ti stent was 4.95 ± 1.12 μm, while the Ra of the Gr–Ti stent was 6.37 ± 0.72 μm. After the adipose stem cells were co-cultured with the scaffold for 4 h and 24 h, it was found that the Gr–Ti scaffold could better promote the early cell adhesion. CCK-8 tests showed that the number of ADSCs on the G–Ti scaffold was significantly higher than that on the Ti scaffold (p < 0.01). The relative growth rate (RGR) of ADSCs in Gr–Ti was grade 0–1 (non-toxic). In the in vivo experiment of repairing a critical bone defect of a rabbit mandible, the bone volume fraction in the Gr–Ti group increased to 49.42 ± 3.28%, which was much higher than that in the Ti group (39.76 ± 3.62%) (p < 0.05). In conclusion, the porous graphene–titanium alloy promotes the proliferation and adhesion of adipose stem cells with multidirectional differentiation potential, which has great potential for the application of bone tissue engineering in repairing bone defects in the future.
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Li P, Li Y, Kwok T, Yang T, Liu C, Li W, Zhang X. A bi-layered membrane with micro-nano bioactive glass for guided bone regeneration. Colloids Surf B Biointerfaces 2021; 205:111886. [PMID: 34091371 DOI: 10.1016/j.colsurfb.2021.111886] [Citation(s) in RCA: 20] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/02/2021] [Revised: 05/27/2021] [Accepted: 05/27/2021] [Indexed: 12/21/2022]
Abstract
Guided bone regeneration (GBR) is widely used to treat oral bone defects. However, the osteogenic effects are limited by the deficiency of the available barrier membranes. In this study, a novel bi-layer membrane was prepared by solvent casting and electrospinning. The barrier layer made of poly (lactic-co-glycolic acid) (PLGA) was smooth and compact, whereas the osteogenic layer consisting of micro-nano bioactive glass (MNBG) and PLGA was rough and porous. The mineralization evaluation confirmed that apatite formed on the membranes in simulated body fluid. Immersion in phosphate-buffered saline led to the degradation of the membranes with proper pH changes. Mechanical tests showed that the bi-layered membranes have stable mechanical properties under dry and wet conditions. The bi-layered membranes have good histocompatibility, and the MNBG/PLGA layer can enhance bone regeneration activity. This was confirmed by cell culture results, expression of osteogenic genes, and immunofluorescence staining of RUNX-related transcription factor 2 and osteopontin. Therefore, the bi-layered membranes could be a promising clinical strategy for GBR surgery.
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Affiliation(s)
- Peiyi Li
- Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510000, PR China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510000, PR China
| | - Yanfei Li
- Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510000, PR China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510000, PR China
| | - Tszyung Kwok
- Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510000, PR China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510000, PR China
| | - Tao Yang
- Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510000, PR China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510000, PR China
| | - Cong Liu
- Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, PR China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, 510006, PR China
| | - Weichang Li
- Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510000, PR China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510000, PR China.
| | - Xinchun Zhang
- Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510000, PR China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510000, PR China.
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16
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Feng KC, Wu YJ, Wang CY, Tu CS, Lin YL, Chen CS, Lai PL, Huang YT, Chen PY. Enhanced mechanical and biological performances of CaO-MgO-SiO 2 glass-ceramics via the modulation of glass and ceramic phases. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2021; 124:112060. [PMID: 33947554 DOI: 10.1016/j.msec.2021.112060] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/25/2020] [Revised: 02/26/2021] [Accepted: 03/20/2021] [Indexed: 12/23/2022]
Abstract
This work reports a new CaO-MgO-SiO2 (CMS) bioactive glass-ceramic, using ZrO2 as a nucleus to modulate the ratios of glass and ceramic phases as a function of sintering temperature. Mg-rich bioactive CMS glass-ceramics exhibit advantages regarding mechanical strength (flexural strength ~190 MPa and compressive strength ~555 MPa), in-vitro and in-vivo biocompatibilities, and bone ingrowth. The high mechanical strengths could be attributed to the CaMgSi2O6 glass-ceramic and lower porosity. X-ray absorption spectra indicate an increased SiO covalent bond via the development of CaMgSi2O6 glass-ceramics. From the in-vitro cytotoxicity and BMSC differentiation assays, the CMS samples sintered above 800 °C exhibited better cell attachment and differentiation, possibly due to structural stability, appropriate pore, and ion release to boost osteogenesis. Compared to hydroxyapatite (HA) ceramics, the CMS glass-ceramics display higher mechanical strengths, biocompatibility, and osteoconductivity. An in-vivo experiment demonstrated a fine bone-ingrowth profile around the CMS implant. This study may further the application of CMS glass-ceramics in bone implants.
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Affiliation(s)
- Kuei-Chih Feng
- Research Center for Intelligent Medical Devices, Ming Chi University of Technology, New Taipei City 24301, Taiwan; Department of Mechanical Engineering, Ming Chi University of Technology, New Taipei City 24301, Taiwan
| | - Yu-Jie Wu
- International Ph.D. Program in Innovative Technology of Biomedical Engineering and Medical Devices, Ming Chi University of Technology, New Taipei City 24301, Taiwan
| | - Chi-Yun Wang
- International Ph.D. Program in Innovative Technology of Biomedical Engineering and Medical Devices, Ming Chi University of Technology, New Taipei City 24301, Taiwan; Bone and Joint Research Center, Chang Gung Memorial Hospital, Taoyuan City 33305, Taiwan
| | - Chi-Shun Tu
- Research Center for Intelligent Medical Devices, Ming Chi University of Technology, New Taipei City 24301, Taiwan; International Ph.D. Program in Innovative Technology of Biomedical Engineering and Medical Devices, Ming Chi University of Technology, New Taipei City 24301, Taiwan; Department of Physics, Fu Jen Catholic University, New Taipei City 24205, Taiwan
| | - Yu-Ling Lin
- School of Medicine, Fu Jen Catholic University, New Taipei City 24205, Taiwan
| | - Cheng-Sao Chen
- Department of Mechanical Engineering, Hwa Hsia University of Technology, New Taipei City 23567, Taiwan
| | - Po-Liang Lai
- Bone and Joint Research Center, Chang Gung Memorial Hospital, Taoyuan City 33305, Taiwan
| | - Yu-Tzu Huang
- School of Medicine, Fu Jen Catholic University, New Taipei City 24205, Taiwan.
| | - Pin-Yi Chen
- Research Center for Intelligent Medical Devices, Ming Chi University of Technology, New Taipei City 24301, Taiwan; International Ph.D. Program in Innovative Technology of Biomedical Engineering and Medical Devices, Ming Chi University of Technology, New Taipei City 24301, Taiwan; Department of Mechanical Engineering, Ming Chi University of Technology, New Taipei City 24301, Taiwan.
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Bueno OMVM, Herrera CL, Bertran CA, San-Miguel MA, Lopes JH. An experimental and theoretical approach on stability towards hydrolysis of triethyl phosphate and its effects on the microstructure of sol-gel-derived bioactive silicate glass. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2021; 120:111759. [PMID: 33545900 DOI: 10.1016/j.msec.2020.111759] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/04/2020] [Revised: 11/16/2020] [Accepted: 11/21/2020] [Indexed: 11/19/2022]
Abstract
The sol-gel method is versatile and one of the well-established synthetic approaches for preparing bioactive glass with improved microstructure. In a successful approach, alkoxide precursors undergo rapid hydrolysis, followed by immediate condensation leading to the formation of three-dimensional gels. On the other hand, a slow kinetics rate for hydrolysis of one or more alkoxide precursors generates a mismatch in the progression of the consecutive reactions of the sol-gel process, which makes it difficult to form homogeneous multicomponent glass products. The amorphous phase separation (APS) into the gel is thermodynamically unstable and tends to transform into a crystalline form during the calcination step of xerogel. In the present study, we report a combined experimental and theoretical method to investigate the stability towards hydrolysis of triethyl phosphate (TEP) and its effects on the mechanism leading to phase separation in 58S bioactive glass obtained via sol-gel route. A multitechnical approach for the experimental characterization combined with calculations of functional density theory (DFT) suggest that TEP should not undergo hydrolysis by water under acidic conditions during the formation of the sol or even in the gel phase. The activation energy barrier (ΔG‡) showed a height of about 20 kcal·mol-1 for the three stages of hydrolysis and the reaction rates calculated for each stage of TEP hydrolysis were kFHR = 7.0 × 10-3s-1, kSHR = 6.8 × 10-3s-1 and kTHR = 3.5 × 10-3s-1. These results show that TEP remains in the non-hydrolyzed form segregated within the xerogel matrix until its thermal decomposition in the calcination step, when P species preferentially associate with calcium ions (labile species) and other phosphate groups present nearby, forming crystalline domains of calcium pyrophosphates permeated by the silica-rich glass matrix. Together, our data expand the knowledge about the synthesis by the sol-gel method of bioactive glass and establishes a mechanism that explains the role played by the precursor source of phosphorus (TEP) in the phase separation, an event commonly observed for these biomaterials.
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Affiliation(s)
- Otto Mao Vargas Machuca Bueno
- Department of Physical Chemistry, Institute of Chemistry, University of Campinas - UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil.
| | - Christian Leonardo Herrera
- Department of Physical Chemistry, Institute of Chemistry, University of Campinas - UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil
| | - Celso Aparecido Bertran
- Department of Physical Chemistry, Institute of Chemistry, University of Campinas - UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil
| | - Miguel Angel San-Miguel
- Department of Physical Chemistry, Institute of Chemistry, University of Campinas - UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil
| | - João Henrique Lopes
- Department of Chemistry, Division of Fundamental Sciences (IEF), Aeronautics Institute of Technology - ITA, 12228-900 Sao Jose dos Campos, SP, Brazil.
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18
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Tetzel L, Guyard M. Saving the lower limb with GlassBONE™ - Successful surgical revision of pseudarthrosis after infected open proximal tibia fracture type IIIC with bioactive glass grafting - A case report. Trauma Case Rep 2020; 31:100382. [PMID: 33426259 PMCID: PMC7779823 DOI: 10.1016/j.tcr.2020.100382] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 12/06/2020] [Indexed: 12/04/2022] Open
Abstract
Background The management of bone defect due to trauma or surgical debridement is a current problem in orthopedic trauma surgery, often complicated by infection and bone nonunion. The graft is one of the most challenging variables in surgical treatment. Bioactive Glass (BAG) as a biocompatible and osteogenic product is a promising bone substitute showing good results in maxillo-facial-, spine surgery and treatment of osteomyelitis. Surprisingly, there is very little data on BAG use in trauma surgery. Case presentation A 51-year-old male patient, involved in a motorcycle accident, suffered an open proximal tibia fracture, type IIIC, of the left leg. Patient was admitted in January of 2013 to a general orthopedic department for surgical treatment. After several surgical revisions due to infection, vascular damage, and bone nonunion, the patient was successfully treated with Masquelet therapy followed by GlassBONE™ grafting (GlassBONE™ 45S5; Norarker). The patient demonstrated excellent results over the course of a two-year follow-up. Conclusions In our experience, GlassBONE™ 45S5 has proven to be an effective bone substitute even in difficult grafting conditions, including multiple surgical revisions for bone nonunion and infection. In our case, at the end of 2 years and 3 months of follow-up, the patient reported no pain, and had no signs of infection. Bone union and full weight bearing was achieved. This case report is oriented by the CARE guidelines for clinical case reports; the patient gave consent for publication.
GlassBONE™ 45S5 has proven to be an effective bone substitute even in difficult grafting conditions. Bone nonunion was successfully treated with Masquelet therapy followed by GlassBONE™ grafting. Bone loss due to fracture or surgical debridement is a current problem in orthopedic trauma surgery. Bone fractures are the most widespread trauma in humans.
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Affiliation(s)
- L Tetzel
- Orthopedic Department, University Children's Hospital Basel, Switzerland
| | - M Guyard
- Orthopedic Department, Centre Hospitalier Saint Joseph Saint Luc, Lyon, France
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19
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Marczewski M, Jurczyk MU, Kowalski K, Miklaszewski A, Wirstlein PK, Jurczyk M. Composite and Surface Functionalization of Ultrafine-Grained Ti23Zr25Nb Alloy for Medical Applications. MATERIALS 2020; 13:ma13225252. [PMID: 33233693 PMCID: PMC7699683 DOI: 10.3390/ma13225252] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 10/24/2020] [Revised: 11/06/2020] [Accepted: 11/18/2020] [Indexed: 12/26/2022]
Abstract
In this study, the ultrafine-grained Ti23Zr25Nb-based composites with 45S5 Bioglass and Ag, Cu, or Zn additions were produced by application of the mechanical alloying technique. Additionally, the base Ti23Zr25Nb alloy was electrochemically modified in the two stages of processing: electrochemical etching in the solution of H3PO4 and HF followed by electrochemical deposition in Ca(NO3)2, (NH4)2HPO4, and HCl. The in vitro cytocompatibility studies were also done with comparison to the commercially pure titanium. The established cell lines of Normal Human Osteoblasts (NHost, CC-2538) and Human Periodontal Ligament Fibroblasts (HPdLF, CC-7049) were used. The culture was conducted among the tested materials. Ultrafine-grained titanium-based composites modified with 45S5 Bioglass and Ag, Cu, or Zn metals have higher biocompatibility than the reference material in the form of a microcrystalline Ti. Proliferation activity was at a stable level with contact with studied materials. In vitro evaluation research showed that the ultrafine-grained Ti23Zr25Nb-based composites with 45S5 Bioglass and Ag, Cu, or Zn additions, with a Young modulus below 50 GPa, can be further used in the biomedical field.
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Affiliation(s)
- Mateusz Marczewski
- Institute of Materials Science and Engineering, Poznan University of Technology, Jana Pawła II 24, 61-138 Poznan, Poland; (K.K.); (A.M.); (M.J.)
- Correspondence: ; Tel.: +48-61-665-3508
| | - Mieczysława U. Jurczyk
- Division of Mother’s and Child’s Health, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, Poland;
| | - Kamil Kowalski
- Institute of Materials Science and Engineering, Poznan University of Technology, Jana Pawła II 24, 61-138 Poznan, Poland; (K.K.); (A.M.); (M.J.)
| | - Andrzej Miklaszewski
- Institute of Materials Science and Engineering, Poznan University of Technology, Jana Pawła II 24, 61-138 Poznan, Poland; (K.K.); (A.M.); (M.J.)
| | - Przemysław K. Wirstlein
- Department of Gynaecology and Obstetrics, Division of Reproduction, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, Poland;
| | - Mieczysław Jurczyk
- Institute of Materials Science and Engineering, Poznan University of Technology, Jana Pawła II 24, 61-138 Poznan, Poland; (K.K.); (A.M.); (M.J.)
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20
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Benetti F, Bueno CRE, Reis-Prado AHD, Souza MT, Goto J, Camargo JMPD, Duarte MAH, Dezan-Júnior E, Zanotto ED, Cintra LTA. Biocompatibility, Biomineralization, and Maturation of Collagen by RTR®, Bioglass and DM Bone® Materials. Braz Dent J 2020; 31:477-484. [PMID: 33146330 DOI: 10.1590/0103-6440202003660] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/12/2020] [Accepted: 08/25/2020] [Indexed: 11/21/2022] Open
Abstract
This study evaluated the biocompatibility, biomineralization, and collagen fiber maturation induced by Resorbable Tissue Replacement (RTR®; β-tricalcium phosphate [TCP]), Bioglass (BIOG; bioactive glass), and DM Bone® (DMB; hydroxyapatite and β-TCP) in vivo. Sixty-four polyethylene tubes with or without (control group; CG) materials (n=8/group/period) were randomly implanted in the subcutaneous tissue of 16 male Wistar rats (four per rat), weighting 250 to 280 g. The rats were killed after 7 and 30 days (n=8), and the specimens were removed for analysis of inflammation using hematoxylin-eosin; biomineralization assay using von Kossa (VK) staining and polarized light (PL); and collagen fiber maturation using picrosirius red (PSR). Nonparametric data were statistically analyzed by Kruskal-Wallis and Dunn tests, and parametric data by one-way ANOVA test (p<0.05). At 7 days, all groups induced moderate inflammation (p>0.05). At 30 days, there was mild inflammation in the BIOG and CG, and moderate inflammation in the RTR and DMB groups, with a significant difference between the CG and RTR (p<0.05). The fibrous capsule was thick at 7 days and predominantly thin at 30 days in all groups. All materials exhibited structures that stained positively for VK and PL. Immature collagen fibers were predominant at 7 and 30 days in all groups (p>0.05), although DMB exhibited more mature fibers than BIOG at 30 days (p<0.05). RTR, BIOG, and DMB were biocompatible, inducing inflammation that reduced over time and biomineralization in the subcutaneous tissue of rats. DMB exhibited more mature collagen fibers than BIOG over a longer period.
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Affiliation(s)
- Francine Benetti
- Department of Restorative Dentistry, School of Dentistry, UFMG - Universidade Federal de Minas Gerais, , Belo Horizonte, MG, Brazil.,Department of Endodontics, School of Dentistry, UNESP - Universidade Estadual Paulista, Araçatuba, SP, Brazil
| | | | | | - Marina Trevelin Souza
- Department of Materials Engineering, Vitreous Materials Laboratory (LaMaV) UFSCar - Universidade Federal de São Carlos, SP, Brazil
| | - Juliana Goto
- Department of Endodontics, School of Dentistry, UNESP - Universidade Estadual Paulista, Araçatuba, SP, Brazil
| | | | - Marco Antônio Húngaro Duarte
- Department of Dentistry, Endodontics and Dental Materials, School of Dentistry, USP - Universidade de São Paulo, Bauru, SP, Brazil
| | - Elói Dezan-Júnior
- Department of Endodontics, School of Dentistry, UNESP - Universidade Estadual Paulista, Araçatuba, SP, Brazil
| | - Edgar Dutra Zanotto
- Department of Materials Engineering, Vitreous Materials Laboratory (LaMaV) UFSCar - Universidade Federal de São Carlos, SP, Brazil
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