Homeobox C6 plays an oncogenic role in bladder cancer

Table 1 Primer sequences

Primer	Sequence (5’-3’)
Hoxc6 (human)-F	CCAGAAAGCCAGTATCCA
Hoxc6 (human)-R	TTCTCCAGTTCCAGGGTC
Gapdh (human)-F	GGAGCGAGATCCCTCCAAAAT
Gapdh (human)-R	GGCTGTTGTCATACTTCTCATGG

Table 2 siRNA sequences

siRNA	Sense sequence (5’ to 3’)	Anti-sense sequence (5’ to 3’)
HOXC6-siRNA-1	CCUCAAUCGCUCAGGAUUUTT	AAAUCCUGAGCGAUUGAGGTT
HOXC6-siRNA-2	CAGAAGAGGAGAAGCAGAATT	UUCUGCUUCUCCUCUUCUGTT
HOXC6-siRNA-3	CUCUCAAACUGCAGACAAATT	UUUGUCUGCAGUUUGAGAGTT

HOXC6: Homeobox C6.

Table 3 The relationship between the protein expression of homeobox C6 and clinicopathological parameters in bladder cancer based on immunohistochemistry

Clinicopathological parameters	n	HOXC6 expression		P value
		Mean	SD
Group
Cancer	154	9.517	2.121	d
Non-cancer	46	3.170	1.784
Gender
Male	133	9.504	2.128	0.848
Female	21	9.600	2.132
Age (years)
< 60	59	9.946	1.992	a
≥ 60	95	9.251	2.165
Pathological T stage
T1 + T2	124	9.565	2.184	0.573
T3 + T4	30	9.320	1.859
Pathological grade
I-II	92	9.544	2.098	0.850
III-IV	62	9.477	2.172

^aP < 0.05.

^dP < 0.001.

BLCA: Bladder cancer; HOXC6: Homeobox C6.