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©The Author(s) 2025.
World J Clin Oncol. Apr 24, 2025; 16(4): 104182
Published online Apr 24, 2025. doi: 10.5306/wjco.v16.i4.104182
Published online Apr 24, 2025. doi: 10.5306/wjco.v16.i4.104182
Table 1 Real-time quantitative PCR primer sequences
| Gene (Rabbit) | Prime | Product length | Login ID |
| GAPDH | F: GCAAAGTGGATGTTGTCGCC | 132 | NM_001082253.1 |
| R: TGATGACCAGCTTCCCGTTC | |||
| FDFT1 | F: AGATTCGGAAAGGGCAAGCA | 223 | NM_001287742.2 |
| R: AACGACAGGTAGATGGGGGA | |||
| TIMP1 | F: TCAACCAGACCACCTTATACC | 296 | NM_003254.3 |
| R: GCATTCCTCACAGCCAACAG | |||
| GADD45B | F: GCCCTGCAAATCCACTTCAC | 165 | NM_015675.4 |
| R: GTTCGTGACCAGGAGACAAT | |||
| LIME1 | F: GGAAGCGCAAGTCGGACAC | 236 | NM_001305654.2 |
| R: CACGTTGGAATAGGTGGCCT | |||
| EHD4 | F: CTTCGAGAACAAGCCCATGA | 161 | NM_139265.4 |
| R: TGCCCTCAGTCTCTCCATACA |
Table 2 Loading list
| Reagent name (μL) | Measuring tube | Standard tube |
| Sample | 20 | |
| Standard liquid | 20 | |
| Reagent I | 128 | 128 |
| Operating fluid | 52 | 52 |
- Citation: Zhang HL, Zhao R, Wang D, Mohd Sapudin SN, Yahaya BH, Harun MSR, Zhang ZW, Song ZJ, Liu YT, Doblin S, Lu P. Candida albicans and colorectal cancer: A paradoxical role revealed through metabolite profiling and prognostic modeling. World J Clin Oncol 2025; 16(4): 104182
- URL: https://www.wjgnet.com/2218-4333/full/v16/i4/104182.htm
- DOI: https://dx.doi.org/10.5306/wjco.v16.i4.104182