Role of octamer transcription factor 4 in proliferation, migration, drug sensitivity, and stemness maintenance of pancreatic cancer cells

Table 1 Small interfering RNA sequences

Name	Sense (5′-3′)	Antisense (5′-3′)
OCT4 (human) siRNA-1#	GAGGCAACCUGGAGAAUUUTT	AAAUUCUCCAGGUUGCCUCTT
OCT4 (human) siRNA-2#	GCGACUAUGCACAACGAGATT	UCUCGUUGUGCAUAGUCGCTT
OCT4 (human) siRNA-3#	CAGAAGGGCAAGCGAUCAATT	UUGAUCGCUUGCCCUUCUGTT
OCT4 (human) siRNA-4#	GCAGAAAGAACUCGAGCAATT	UUGCUCGAGUUCUUUCUGCTT
Negative control	UUCUCCGAACGUGUCACGUTT	ACGUGACACGUUCGGAGAATT

siRNA: Small interfering RNA; OCT4: Octamer transcription factor 4.

Table 2 Antibodies used

	Item No.	Supplier	Country
AKT Monoclonal antibody	60203-2-Ig	Proteintech	China
Phospho-AKT (Ser473) Polyclonal antibody	28731-1-AP	Proteintech	China
PI3 Kinase p85 Alpha Monoclonal antibody	60225-1-Ig	Proteintech	China
Phospho-PI3K p85 (Tyr458) [Tyr467]/p55 (Tyr199) Antibody	AF3242	Affinity	China
mTOR Monoclonal antibody	66888-1-Ig	Proteintech	China
Phospho-mTOR (Ser2448) Monoclonal antibody	67778-1-Ig	Proteintech	China
HRP conjugated Goat Anti-Mouse IgG (H+L)	GB23301	Servicebio	China
HRP conjugated Goat Anti-Rabbit IgG (H+L)	GB23303	Servicebio	China

Table 3 Expression of Octamer transcription factor 4 and CD133 in pancreatic and paracancer tissues

Clinicopathological characteristic	n	CD133 high	CD133 low	P value	OCT4 high	OCT4 low	P value
Gender
Female	37	26	11	0.0336	35	28	0.0244
Male	63	46	17		23	14
Age
< 65	60	45	15	0.0206	36	24	0.0098
≥ 65	40	29	11		22	18
Pathologic grading
I-II	69	48	21	0.1138	38	31	0.4874
II-IV	31	26	5		20	11
TNM
I-II	79	61	18	0.0059	46	33	0.0115
III	21	13	8		12	9
Pathologic staging
1A-1B	44	36	8	0.0435	25	19	0.3277
2A-2B	56	39	17	33	23

OCT4: Octamer transcription factor 4.