Nomogram based on multimodal magnetic resonance combined with B7-H3mRNA for preoperative lymph node prediction in esophagus cancer

Table 1 Characteristics of patients in the cohort, n (%)

Characteristic	LN metastasis (+)	LN metastasis (-)	P value
Age, mean ± SD	70.18 ± 6.75	71.92 ± 7.49	0.39
Tumor size, mean ± SD	3.89 ± 1.55	3.18 ± 1.64	0.24
Sex			0.54
Male	10 (66.67)	13 (76.47)
Female	5 (33.33)	4 (23.53)
CEA			0.99
0-5 ng/mL	15 (99.99)	16 (94.12)
> 5 ng/mL	0 (0.01)	1 (5.88)
Squamous cell carcinoma antigen			0.61
0-2.7 ng/mL	12 (80.0)	14 (82.35)
> 2.7 ng/mL	3 (20.00)	3 (17.65)
Location			0.99
Upper mediastinal	2 (13.33)	1 (5.88)
Middle mediastinal	6 (40.00)	8 (47.06)
Lower mediastinal	6 (40.00)	8 (47.06)
Abdominal	1 (6.67)	0 (0.01)
Histologic grade			0.40
Well differentiated	0 (0.01)	2 (11.77)
Moderately differentiated	11 (73.33)	11 (64.71)
Poorly differentiated	4 (26.67)	4 (23.53)
T stage			0.01
1	3 (20.00)	10 (58.82)
2	12 (80.00)	5 (29.41)
3	0 (0.01)	2 (11.77)
CT-reported LN status			0.16
0-10.0 mm	6 (40.00)	11 (64.71)
> 10.0 mm	9 (60.00)	6 (35.29)

LN: Lymph node; T: Tumor; CEA: Carcinoembryonic antigen; CT: Computed tomography.

Table 2 The relationship between B7-H3 expression and clinical characteristics of esophageal cancer patients

Clinical characteristic	Number of sample	B7-H3mRNA	Statistic	P value
Sex
Male	23	2.47 ± 0.51	0.06	0.80
Female	9	2.52 ± 0.40
T			F statistic
1	13	2.23 ± 0.32	3.309	0.05
2	17	2.66 ± 0.53
3	2	2.59 ± 0.12
N
0	17	2.29 ± 0.35	2.50	0.02
1-3	15	2.70 ± 0.53

T: Tumor; N: Node.

Table 3 Values of various coefficients in the nomogram

Variables	Coef	95%CI
T1.WM	-0.5717	(-2.85, -0.25)
T1-wavelet-LHL_firstorder_Median
T1.W.HSAHGE	0.22695	(-0.21, -2.91)
HLH_glszm_SmallAreaHighGrayLevelEmphasis
T1.W.CT	-2.1616	(-3.72, -0.87)
T1-wavelet-HHL_gicm_ClusterTendency
T1.W.R	0.49155	(-1.02, -3.32)
T1-wavelet
HHH_firstorder_InterquartileRange	-4.1807	(-4.97, -0.68)
T2.O.DE
T2-original_gldm_DependenceEntropy	47.4461	(-0.67, -3.38)
T2.W.gl
T2-wavelet-HLH_gicm_Idn	3.69379	(0.18, -4.34)
B7.H3
CT	1.46262	(-1.98, -4.90)

The coefficient values of individual independent factors in the developed column chart are shown in the figure. The selected radiomic features and their abbreviations in the nomogram are presented in the table. All predictive factors have a P value < 0.05, indicating statistical significance. CT: Computed tomography.

Table 4 Receiver operating characteristic curve of the dataset

Model	AUC (95%CI)	Sensitivity	Specificity	Youden index
Radiomics	0.627 (0.427-0.828)	0.667	0.647	0.314
Nomogram	0.765 (0.598-0.931)	0.800	0.706	0.506
CT	0.624 (0.426-0.821)	0.600	0.647	0.247
B7-H3mRNA	0.718 (0.528-0.907)	0.733	0.706	0.439

CT: Computed tomography; AUC: Area under the curve.