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Wójciak M, Paduch R, Drozdowski P, Żuk M, Wójciak W, Tyszczuk-Rotko K, Feldo M, Sowa I. Ultra-Performance Liquid Chromatography and Mass Spectrometry Characterization, and Antioxidant, Protective, and Anti-Inflammatory Activity, of the Polyphenolic Fraction from Ocimum basilicum. Molecules 2024; 29:5043. [PMID: 39519685 PMCID: PMC11547609 DOI: 10.3390/molecules29215043] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/07/2024] [Revised: 10/23/2024] [Accepted: 10/23/2024] [Indexed: 11/16/2024] Open
Abstract
Ocimum basilicum is a valuable plant widely consumed worldwide and considered a rich source of polyphenols. This study examined the impact of the polyphenolic fraction isolated from basil (ObF) on human normal colon epithelial cells and human colorectal adenocarcinoma cells, evaluating its anti-inflammatory and protective activity against oxidative stress. The phytochemical characterization of the fraction was performed using ultra-performance liquid chromatography (UPLC) with a photodiode detector (DAD) and mass spectrometry (MS). UPLC-DAD-MS revealed that ObF predominantly contains caffeic acid derivatives, with rosmarinic acid and chicoric acid being the most abundant. The fraction demonstrated high antioxidant potential, as shown by DPPH assays, along with significant reducing power (FRAP). Furthermore, it prevented the depletion of antioxidant enzymes, including superoxide dismutase and catalase, and decreased malonylodialdehyde (MDA) in induced oxidative stress condition. Additionally, it exhibited a significant protective effect against H2O2-induced cytotoxicity in human normal colon epithelial cells. Although it had no impact on the viability of adenocarcinoma cells, it significantly reduced IL-1β levels in the neoplastic microenvironment. Our study demonstrated that basil polyphenols provide significant health benefits due to their antioxidant and protective activities.
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Affiliation(s)
- Magdalena Wójciak
- Department of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, Poland; (M.Ż.); (W.W.); (I.S.)
| | - Roman Paduch
- Department of Virology and Immunology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, 20-033 Lublin, Poland;
- Department of General and Pediatric Ophthalmology, Medical University of Lublin, 20-079 Lublin, Poland
| | - Piotr Drozdowski
- Department of Plastic Surgery, Specialist Medical Centre, 57-320 Polanica-Zdrój, Poland;
| | - Magdalena Żuk
- Department of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, Poland; (M.Ż.); (W.W.); (I.S.)
| | - Weronika Wójciak
- Department of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, Poland; (M.Ż.); (W.W.); (I.S.)
| | - Katarzyna Tyszczuk-Rotko
- Faculty of Chemistry, Institute of Chemical Sciences, Maria Curie-Skłodowska University in Lublin, 20-031 Lublin, Poland;
| | - Marcin Feldo
- Department of Vascular Surgery, Medical University of Lublin, 20-081 Lublin, Poland;
| | - Ireneusz Sowa
- Department of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, Poland; (M.Ż.); (W.W.); (I.S.)
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Vidaković V, Vujić B, Jadranin M, Novaković I, Trifunović S, Tešević V, Mandić B. Qualitative Profiling, Antioxidant and Antimicrobial Activities of Polar and Nonpolar Basil Extracts. Foods 2024; 13:2993. [PMID: 39335921 PMCID: PMC11431458 DOI: 10.3390/foods13182993] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/05/2024] [Revised: 09/11/2024] [Accepted: 09/18/2024] [Indexed: 09/30/2024] Open
Abstract
Basil (Ocimum basilicum L.) is a widely used culinary herb. In this study, ethanol, dichloromethane, and sunflower oil were used separately as solvents with distinct polarities for the extraction of basil aerial parts to simulate the different polarity conditions in domestic food processing. The oil extract (OE) was re-extracted with acetonitrile, and the chemical composition, antioxidant potential, and antimicrobial activities of the ethanol (EE), dichloromethane (DCME), and acetonitrile (ACNE) extracts were determined. A total of 109 compounds were tentatively identified in EE, DCME, and ACNE by HPLC-DAD/ESI-ToF-MS. Fatty acids were present in all extracts. Phenolic acids and flavonoids dominated in EE. DCME was characterised by triterpenoid acids, while diterpenoids were mainly found in ACNE. The extracts were analysed for their antioxidant capacity using the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay. EE and DCME showed significant radical scavenging potential. Antimicrobial activity was explored in eight bacterial, two yeast, and one fungal species. All extracts exhibited high antifungal activity, comparable to or better than that of the commercial drug nistatin. Antibacterial activities were notable for EE and ACNE, while DCME showed no activity against bacteria in the applied concentration ranges. The different polarities of the solvents led to distinctive phytochemical compositions and bioactivities in the extracts.
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Affiliation(s)
- Vera Vidaković
- Department of Ecology, University of Belgrade—Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, Bulevar despota Stefana 142, 11108 Belgrade, Serbia;
| | - Bojan Vujić
- University of Belgrade—Faculty of Chemistry, Studentski trg 12–16, 11000 Belgrade, Serbia; (B.V.); (S.T.); (V.T.)
| | - Milka Jadranin
- University of Belgrade—Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Njegoševa 12, 11000 Belgrade, Serbia; (M.J.); (I.N.)
| | - Irena Novaković
- University of Belgrade—Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Njegoševa 12, 11000 Belgrade, Serbia; (M.J.); (I.N.)
| | - Snežana Trifunović
- University of Belgrade—Faculty of Chemistry, Studentski trg 12–16, 11000 Belgrade, Serbia; (B.V.); (S.T.); (V.T.)
| | - Vele Tešević
- University of Belgrade—Faculty of Chemistry, Studentski trg 12–16, 11000 Belgrade, Serbia; (B.V.); (S.T.); (V.T.)
| | - Boris Mandić
- University of Belgrade—Faculty of Chemistry, Studentski trg 12–16, 11000 Belgrade, Serbia; (B.V.); (S.T.); (V.T.)
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Polaki S, Stamatelopoulou V, Kotsou K, Chatzimitakos T, Athanasiadis V, Bozinou E, Lalas SI. Exploring Conventional and Green Extraction Methods for Enhancing the Polyphenol Yield and Antioxidant Activity of Hyssopus officinalis Extracts. PLANTS (BASEL, SWITZERLAND) 2024; 13:2105. [PMID: 39124223 PMCID: PMC11313759 DOI: 10.3390/plants13152105] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/31/2024] [Revised: 07/24/2024] [Accepted: 07/27/2024] [Indexed: 08/12/2024]
Abstract
Hyssopus officinalis L. (HO) is, as one of the most prevalently utilized plants, used in traditional medicine to cure various diseases as well as the in food and cosmetic industries. Moreover, HO is a rich source of polyphenols with potent antioxidant properties. However, the studies on the extraction of such compounds from HO are scanty and sparse. This study aims to optimize the extraction of polyphenols and maximize the antioxidant activity in HO extracts. A comprehensive experimental design was employed, encompassing varied extraction parameters to determine the most effective ones. Alongside conventional stirring (ST), two green approaches, the ultrasonic treatment (US) and the pulsed electric field (PEF), were explored, either alone or in combination. The extracted polyphenolic compounds were identified with a high-performance liquid chromatography-diode array detector (HPLC-DAD). According to the results, the employment of ST along with an ethanolic solvent at 80 °C for 150 min seems beneficial in maximizing the extraction of polyphenols from HO, resulting in extracts with enhanced antioxidant activity. The total polyphenol was noted at 70.65 ± 2.76 mg gallic acid equivalents (GAE)/g dry weight (dw) using the aforementioned techniques, and the antioxidant activity was noted as 582.23 ± 16.88 μmol ascorbic acid equivalents (AAE)/g dw (with FRAP method) and 343.75 ± 15.61 μmol AAE/g dw (with the DPPH method). The as-prepared extracts can be utilized in the food and cosmetics industries to bestow or enhance the antioxidant properties of commercial products.
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Affiliation(s)
| | | | | | - Theodoros Chatzimitakos
- Department of Food Science & Nutrition, University of Thessaly, Terma N. Temponera Str., 43100 Karditsa, Greece; (S.P.); (V.S.); (K.K.); (V.A.); (E.B.); (S.I.L.)
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Benedec D, Oniga I, Hanganu D, Vlase AM, Ielciu I, Crișan G, Fiţ N, Niculae M, Bab T, Pall E, Vlase L. Revealing the Phenolic Composition and the Antioxidant, Antimicrobial and Antiproliferative Activities of Two Euphrasia sp. Extracts. PLANTS (BASEL, SWITZERLAND) 2024; 13:1790. [PMID: 38999630 PMCID: PMC11244224 DOI: 10.3390/plants13131790] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/26/2024] [Revised: 06/26/2024] [Accepted: 06/26/2024] [Indexed: 07/14/2024]
Abstract
The species of the genus Euphrasia present important medicinal potential according to their traditional uses. However, few studies aim to sustain this fact by scientific evidence. The present study aimed to explore the phytochemical profile and investigate the antioxidant, antimicrobial and antiproliferative potential of E. officinalis subsp. pratensis Fr. (EO) and E. stricta J.P.Wolff ex J.F.Lehm (ES). The tested samples consisted of ethanolic extracts. The identification and quantification of phenolic compounds were performed using spectrophotometric and LC-MS/MS methods. The antioxidant capacity was evaluated using the DPPH, FRAP and xanthine oxidase methods. Antimicrobial properties were screened using disk diffusion, broth microdilution and anti-biofilm assays, while antiproliferative potential was assessed on a colorectal adenocarcinoma human cancer cell line (DLD-1). The LC-MS/MS analysis showed chlorogenic acid and rutin as the dominant constituents in the tested extracts. The antioxidant activity assays showed important capacity for both samples; in vitro antimicrobial and anti-biofilm properties were exhibited, especially against Gram-positive bacteria, and an important inhibitory potential was observed on the proliferation of the DLD-1 cell line. The findings in the present study contribute to the recommendation of EO and ES for the prevention and treatment of oxidative stress-related pathologies, cancer and microbial infections.
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Affiliation(s)
- Daniela Benedec
- Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania; (D.B.); (I.O.); (T.B.)
| | - Ilioara Oniga
- Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania; (D.B.); (I.O.); (T.B.)
| | - Daniela Hanganu
- Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania; (D.B.); (I.O.); (T.B.)
| | - Ana-Maria Vlase
- Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania; (A.-M.V.); (I.I.); (G.C.)
| | - Irina Ielciu
- Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania; (A.-M.V.); (I.I.); (G.C.)
| | - Gianina Crișan
- Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania; (A.-M.V.); (I.I.); (G.C.)
| | - Nicodim Fiţ
- Department of Paraclinical Sciences, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania;
| | - Mihaela Niculae
- Department of Clinical Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania; (M.N.); (E.P.)
| | - Timea Bab
- Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania; (D.B.); (I.O.); (T.B.)
- PlantExtrakt Ltd., 407059 Cluj-Napoca, Romania
| | - Emoke Pall
- Department of Clinical Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania; (M.N.); (E.P.)
| | - Laurian Vlase
- Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania;
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Atazhanova G, Ishmuratova M, Levaya Y, Smagulov M, Lakomkina Y. The Genus Hyssopus: Traditional Use, Phytochemicals and Pharmacological Properties. PLANTS (BASEL, SWITZERLAND) 2024; 13:1683. [PMID: 38931115 PMCID: PMC11207324 DOI: 10.3390/plants13121683] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/15/2024] [Revised: 05/24/2024] [Accepted: 06/05/2024] [Indexed: 06/28/2024]
Abstract
According to modern concepts, the genus Hyssopus L. includes seven plant species (Hyssopus ambiguus (Trautv.) Iljin ex Prochorov. & Lebel; Hyssopus cuspidatus Boriss; Hyssopus latilabiatus C.Y.Wu & H.W. Li; Hyssopus macranthus Boriss.; Hyssopus officinalis L.; Hyssopus seravschanicus (Dubj.) Pazij; Hyssopus subulifolius (Rech.f.) Rech.f.). The plants are rich in various groups of biologically active substances with a wide spectrum of pharmacological action. This review presents a modern comprehensive overview of the botanical research, extraction methods, chemical composition and pharmacological activity of plants of the genus Hyssopus L. As a result of the review, it was established that the chemical composition of plant extracts of the genus Hyssopus L. depends on various factors (place of growth, weather conditions, chemotypes, extraction methods, etc.). For the further use of the plants, the extraction methods and low-molecular metabolites isolated from them (mono- and sesquiterpenoids, flavonoids, alkaloids, etc.) are discussed. The data from the review provide an assessment of the relevance.
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Affiliation(s)
- Gayane Atazhanova
- Research Park of Biotechnology and Eco-Monitoring, Karaganda Buketov University, Universitetskaya Street, 28, Karaganda 100028, Kazakhstan; (G.A.); (M.S.)
- School of Pharmacy, Karaganda Medical University, Gogol Street, 40, Karaganda 100017, Kazakhstan;
| | - Margarita Ishmuratova
- Research Park of Biotechnology and Eco-Monitoring, Karaganda Buketov University, Universitetskaya Street, 28, Karaganda 100028, Kazakhstan; (G.A.); (M.S.)
| | - Yana Levaya
- Research Park of Biotechnology and Eco-Monitoring, Karaganda Buketov University, Universitetskaya Street, 28, Karaganda 100028, Kazakhstan; (G.A.); (M.S.)
- School of Pharmacy, Karaganda Medical University, Gogol Street, 40, Karaganda 100017, Kazakhstan;
| | - Marlen Smagulov
- Research Park of Biotechnology and Eco-Monitoring, Karaganda Buketov University, Universitetskaya Street, 28, Karaganda 100028, Kazakhstan; (G.A.); (M.S.)
| | - Yekaterina Lakomkina
- School of Pharmacy, Karaganda Medical University, Gogol Street, 40, Karaganda 100017, Kazakhstan;
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Oalđe Pavlović M, Kolarević S, Đorđević Aleksić J, Vuković-Gačić B. Exploring the Antibacterial Potential of Lamiaceae Plant Extracts: Inhibition of Bacterial Growth, Adhesion, Invasion, and Biofilm Formation and Degradation in Pseudomonas aeruginosa PAO1. PLANTS (BASEL, SWITZERLAND) 2024; 13:1616. [PMID: 38931048 PMCID: PMC11207635 DOI: 10.3390/plants13121616] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/30/2024] [Revised: 06/07/2024] [Accepted: 06/08/2024] [Indexed: 06/28/2024]
Abstract
In response to the global rise in antibiotic resistance and the prevalence of bacterial biofilm-related infections, the antibacterial efficacy of methanolic, ethanolic, and aqueous extracts of 18 Lamiaceae plants from Serbia was evaluated. The total coumarins and triterpenes were detected spectrophotometrically, while a microdilution assay measured their effects on bacterial growth. Additionally, the impact of these extracts was assessed on Pseudomonas aeruginosa PAO1 adhesion and invasion in human fibroblasts and biofilm formation and degradation. The alcoholic extracts had the highest phytochemical content, with Teucrium montanum and Lavandula angustifolia being the richest in coumarins and triterpenes, respectively. Gram-positive bacteria, particularly Bacillus subtilis, were more susceptible to the extracts. Hyssopus officinalis ethanolic and Sideritis scardica methanolic extracts inhibited bacterial growth the most efficiently. Although the extracts did not inhibit bacterial adhesion, most ethanolic extracts significantly reduced bacterial invasion. Origanum vulgare and H. officinalis ethanolic extracts significantly inhibited biofilm formation, while Teucrium chamaedrys extract was the most active in biofilm degradation. This study significantly contributes to the literature by examining the antibacterial activity of Lamiaceae extracts, addressing major literature gaps, and underscoring their antibacterial potential, particularly Satureja montana and O. vulgare ethanolic extracts, linking their efficacy to coumarins and triterpenes.
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Affiliation(s)
- Mariana Oalđe Pavlović
- University of Belgrade—Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski Trg 16, 11000 Belgrade, Serbia;
| | - Stoimir Kolarević
- University of Belgrade—Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia;
| | - Jelena Đorđević Aleksić
- University of Belgrade—Institute for Multidisciplinary Research, Kneza Višeslava 1, 11000 Belgrade, Serbia;
| | - Branka Vuković-Gačić
- University of Belgrade—Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski Trg 16, 11000 Belgrade, Serbia;
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Pruccoli L, Nicolini B, Lianza M, Teti G, Falconi M, Tarozzi A, Antognoni F. Antioxidant and Anti-Melanogenesis Effects of Teucrium chamaedrys L. Cell Suspension Extract and Its Main Phenylethanoid Glycoside in B16-F10 Cells. PLANTS (BASEL, SWITZERLAND) 2024; 13:808. [PMID: 38592786 PMCID: PMC10974463 DOI: 10.3390/plants13060808] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/13/2024] [Revised: 03/06/2024] [Accepted: 03/07/2024] [Indexed: 04/11/2024]
Abstract
Teucrium chamaedrys L. is a typical European-Mediterranean species of the genus Teucrium. Among the phenolic compounds belonging to phenylethanoid glycosides (PGs), teucrioside (TS) is only found in this species, and it was previously demonstrated to be produced by in vitro-elicited cell cultures at levels higher than those found in leaves. However, T. chamaedrys cell suspension extracts (Cell-Ex) and pure TS have not been investigated yet for any biological effects. In this study, we evaluated the antioxidant and anti-melanogenesis activity of both Cell-Ex and TS in B16-F10 mouse melanoma cells. The results showed that Cell-Ex inhibited the reactive oxygen species formation evoked in B16-F10 cells by tert-butyl hydroperoxide and 5 J/cm2 of UVA, as well as the melanin increase stimulated by α-MSH or 20 J/cm2 of UVA. In parallel, a TS concentration equivalent to that present in Cell-Ex recorded the same biological effect profile, suggesting the main contribution of TS to the antioxidant and anti-melanogenic properties of Cell-Ex. Both Cell-Ex and TS also modulated the melanogenesis pathway through their ability to inhibit the tyrosinase activity both in a cell-free system and in B16-F10 cells stimulated by α-MSH. These results support the potential cosmeceutical use of Cell-Ex for protection against photooxidative damage and hyperpigmentation.
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Affiliation(s)
- Letizia Pruccoli
- Department for Life Quality Studies, University of Bologna, 47921 Rimini, Italy; (L.P.); (B.N.); (M.L.); (F.A.)
| | - Benedetta Nicolini
- Department for Life Quality Studies, University of Bologna, 47921 Rimini, Italy; (L.P.); (B.N.); (M.L.); (F.A.)
| | - Mariacaterina Lianza
- Department for Life Quality Studies, University of Bologna, 47921 Rimini, Italy; (L.P.); (B.N.); (M.L.); (F.A.)
| | - Gabriella Teti
- Department of Biomedical and Neuromotor Sciences, University di Bologna, 40126 Bologna, Italy;
| | - Mirella Falconi
- Department of Medical and Surgical Sciences, University di Bologna, 40126 Bologna, Italy;
| | - Andrea Tarozzi
- Department for Life Quality Studies, University of Bologna, 47921 Rimini, Italy; (L.P.); (B.N.); (M.L.); (F.A.)
- Biostructures and Biosystems National Institute (INBB), 00136 Rome, Italy
| | - Fabiana Antognoni
- Department for Life Quality Studies, University of Bologna, 47921 Rimini, Italy; (L.P.); (B.N.); (M.L.); (F.A.)
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Bilušić T, Šola I, Čikeš Čulić V. Identification of Flavonoids, Antioxidant and Antiproliferative Activity of Aqueous Infusions of Calendula officinalis L., Chelidonium majus L., Teucrium chamaedrys L. and Alchemilla vulgaris L. Food Technol Biotechnol 2024; 62:49-58. [PMID: 38601959 PMCID: PMC11002451 DOI: 10.17113/ftb.62.01.24.8175] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/03/2023] [Accepted: 12/20/2023] [Indexed: 04/12/2024] Open
Abstract
Research background The current changes in the global economy, characterised by the climate crisis and the economic and health impact of the COVID-19 pandemic, have led to a significant demand for medicinal herbs. This trend is expected to increase significantly by 2050. In this study, we investigated the biopotential of aqueous infusions of four medicinal plants: Calendula officinalis, Chelidonium majus, Teucrium chamaedrys and Alchemilla vulgaris. Experimental approach The flavonoid analysis of the aqueous infusions of the selected plants was carried out using the RP-HPLC technique. The antiproliferative activity of the prepared aqueous plant infusions was analysed against three human cancer cell lines (MDA-MD-231, T24 and A549), while the antioxidant potential was measured using three antioxidant methods (DPPH, FRAP and Rancimat assay). Results and conclusions T. chamaedrys had the highest total phenolics (expressed as GAE (2061±42) mg/L), free radical scavenging activity (IC50=1.9 mg/mL) and Fe(III) reducing antioxidant power (expressed as FeCl2 (9798±27) mg/L). At a concentration of 1 mg/mL, the antiproliferation of T24 by C. majus was 96 % and of MDA-MD-231 cells by A. vulgaris was 75 % after 72 h. After principal component analysis, T. chamaedrys and C. majus were grouped together. Quercetin glucoside and antioxidant capacity (DPPH) contributed the most to differentiate these infusions from the other two. Novelty and scientific contribution This study represents a comparative analysis of the biopotential of four medicinal plants. A new RP-HPLC method was developed to separate the flavonoids in the herbal infusions. This is the first report on the presence of kaempferol-3-O-rutinoside in C. officinalis and isorhamnetin-3-O-rutinoside in A. vulgaris aqueous infusion. For the first time, C. majus has been shown to contribute to the oxidative stability of edible oil. Furthermore, this is the first comparative study on the antiproliferative activity of selected medicinal plants against the cell lines MDA-MD-231, T24 and A549.
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Affiliation(s)
- Tea Bilušić
- Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia
| | - Ivana Šola
- Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102 a, 10000 Zagreb, Croatia
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Ndayambaje M, Wahnou H, Sow M, Chgari O, Habyarimana T, Karkouri M, Limami Y, Naya A, Oudghiri M. Exploring the multifaceted effects of Ammi visnaga: subchronic toxicity, antioxidant capacity, immunomodulatory, and anti-inflammatory activities. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH. PART A 2024; 87:150-165. [PMID: 38037686 DOI: 10.1080/15287394.2023.2289430] [Citation(s) in RCA: 11] [Impact Index Per Article: 11.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/02/2023]
Abstract
Ammi visnaga (A. visnaga) is an annual herb that has been used in traditional medicine to treat various ailments attributed to the presence of its bioactive compounds. The purpose of this study was to identify and examine the phytochemical properties of the hydroalcoholic extract of A. visnaga using in vitro and in vivo models. Our findings demonstrated that the extract contained a variety of beneficial components, including phenols, flavonoids, tannins, coumarins, saponins, khellin, and visnagin. The total polyphenolic content and total flavonoid content were 23.26 mg/GAE/g dry weight and 13.26 mg/GAE/g dry weight, respectively. In vitro tests demonstrated that the extract possessed antioxidant properties as evidenced by the ability to scavenge free radicals, including DPPH, ABTS, nitric oxide (NO), phosphomolybdate, and ferric-reducing antioxidant power (FRAP). Further, the extract was found to inhibit hydrogen peroxide (H2O2)-induced hemolysis. In a 90-d in vivo study, female Wistar rats were administered 1 g/kg of A. visnaga extract orally resulting in a significant increase in total white blood cell count. Although morphological changes were observed in the liver, no marked alterations were noted in kidneys and spleen. In a female Swiss albino mice model of acetic acid-induced vascular permeability, A. visnaga significantly inhibited extravasations of Evans blue at doses of 0.5 or 1 g/kg with inhibition percentages of 51 and 65%, respectively, blocking tissue necrosis. The extract also demonstrated potential immunomodulatory properties in mice by enhancing antibody production in response to antigens. In silico molecular docking studies demonstrated a strong affinity between khellin or visnagin and immunomodulatory proteins, NF-κB, p52, and TNF-α. These findings suggest that A. visnaga may be considered a beneficial antioxidant with immunomodulatory properties and might serve as a therapeutic agent to combat certain diseases.
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Affiliation(s)
- Martin Ndayambaje
- Laboratory of Immunology and Biodiversity, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Casablanca, Morocco
| | - Hicham Wahnou
- Laboratory of Immunology and Biodiversity, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Casablanca, Morocco
| | - Marieme Sow
- Laboratory of Immunology and Biodiversity, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Casablanca, Morocco
| | - Oumaima Chgari
- Laboratory of Immunology and Biodiversity, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Casablanca, Morocco
| | | | - Mehdi Karkouri
- Cellular and Molecular Pathology Laboratory, Faculty of Medicine and Pharmacy, Hassan II University, Casablanca, Morocco
| | - Youness Limami
- Laboratory of Immunology and Biodiversity, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Casablanca, Morocco
- Laboratory of Health Sciences and Technologies, Higher Institute of Health Sciences, Hassan First University of Settat, Settat, Morocco
| | - Abdallah Naya
- Laboratory of Immunology and Biodiversity, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Casablanca, Morocco
| | - Mounia Oudghiri
- Laboratory of Immunology and Biodiversity, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Casablanca, Morocco
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Michalak M, Błońska-Sikora E, Stryjecka M, Zagórska-Dziok M, Klimek-Szczykutowicz M, Szopa A. Phytochemical Profile and Antioxidant and Protective Activities of Various Types of Extracts from Hyssopus officinalis L. and Grindelia robusta Nutt. Herb Grown in Poland. Curr Top Med Chem 2024; 24:2238-2253. [PMID: 39253917 DOI: 10.2174/0115680266319052240819104310] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/20/2024] [Revised: 06/03/2024] [Accepted: 07/24/2024] [Indexed: 09/11/2024]
Abstract
INTRODUCTION The available literature indicates that Hyssopus officinalis and Grindelia robusta are raw materials with great potential for use in prevention and therapy. Therefore, the aims of this study were to assess the phytochemical profile and antioxidant and cytoprotective properties of extracts prepared using various solvents, additionally taking into account different methods of drying the plant material. METHODS Hydrodistilled oil was analysed by GC-MS. The chemical composition of the extracts was estimated by spectrophotometry and the HPLC-DAD method. Antioxidant activity was evaluated using DPPH and FRAP and measuring the intracellular level of ROS. Alamar Blue and Neutral Red tests were used to assess the cytotoxicity of the extracts on skin cells - keratinocytes and fibroblasts. RESULTS The major components of hyssop essential oil were cis- (44.9%) and trans- (18.2%) pinocamphone, while borneol (16.1%), and α-pinene (12.0%) were predominant in grindelia essential oil. Flavonoids were dominant in the extracts (water:ethanol, water:methanol, and water: glycerol) from hot-air dried hyssop herb, while phenolic acids were the predominant compounds in the grindelia herb extracts. The water:ethanol hyssop extract had the highest total content of flavonoids (42.26 mg CE/mL), among which isoquercitrin and rutin were present in the highest quantities (32.61 mg/mL and 21.47 mg/mL, respectively). In the case of grindelia, the highest total phenolic acid content (26.24 mg CAE/mL) was recorded in the water:ethanol extract, and the dominant compounds among them were 1,5-dicaffeoylquinic and chlorogenic acid (10.85 and 6.39 mg/mL, respectively). The water:ethanol extract from both plants also exhibited the highest antioxidant activity in the DPPH and FRAP tests (79.19% and 1.39 mmol/L, respectively, for grindelia and 67.61% and 1.04 mmol/L for hyssop) and was most effective at reducing the level of ROS in cells. In addition, water:ethanol extracts may have a positive impact on the viability of skin cells in vitro. CONCLUSION Water:ethanol extracts from H. officinalis and G. robusta herb are promising sources of active compounds and may find application as natural materials with valuable biological properties, which require further in vitro and in vivo testing.
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Affiliation(s)
- Monika Michalak
- Department of Pharmaceutical Sciences, Medical College, Jan Kochanowski University in Kielce, Poland
| | - Ewelina Błońska-Sikora
- Department of Pharmaceutical Sciences, Medical College, Jan Kochanowski University in Kielce, Poland
| | - Małgorzata Stryjecka
- Institute of Human Nutrition and Agriculture, The University College of Applied Sciences in Chełm, Poland
| | - Martyna Zagórska-Dziok
- Department of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Poland
| | | | - Agnieszka Szopa
- Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, Kraków, Poland
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Azizah NS, Irawan B, Kusmoro J, Safriansyah W, Farabi K, Oktavia D, Doni F, Miranti M. Sweet Basil ( Ocimum basilicum L.)-A Review of Its Botany, Phytochemistry, Pharmacological Activities, and Biotechnological Development. PLANTS (BASEL, SWITZERLAND) 2023; 12:4148. [PMID: 38140476 PMCID: PMC10748370 DOI: 10.3390/plants12244148] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/03/2023] [Revised: 12/09/2023] [Accepted: 12/11/2023] [Indexed: 12/24/2023]
Abstract
An urgent demand for natural compound alternatives to conventional medications has arisen due to global health challenges, such as drug resistance and the adverse effects associated with synthetic drugs. Plant extracts are considered an alternative due to their favorable safety profiles and potential for reducing side effects. Sweet basil (Ocimum basilicum L.) is a valuable plant resource and a potential candidate for the development of pharmaceutical medications. A single pure compound or a combination of compounds exhibits exceptional medicinal properties, including antiviral activity against both DNA and RNA viruses, antibacterial effects against both Gram-positive and Gram-negative bacteria, antifungal properties, antioxidant activity, antidiabetic potential, neuroprotective qualities, and anticancer properties. The plant contains various phytochemical constituents, which mostly consist of linalool, eucalyptol, estragole, and eugenol. For centuries, community and traditional healers across the globe have employed O. basilicum L. to treat a wide range of ailments, including flu, fever, colds, as well as issues pertaining to digestion, reproduction, and respiration. In addition, the current research presented underscores the significant potential of O. basilicum-related nanotechnology applications in addressing diverse challenges and advancing numerous fields. This promising avenue of exploration holds great potential for future scientific and technological advancements, promising improved utilization of medicinal products derived from O. basilicum L.
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Affiliation(s)
- Nabilah Sekar Azizah
- Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (N.S.A.); (B.I.); (J.K.); (F.D.)
| | - Budi Irawan
- Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (N.S.A.); (B.I.); (J.K.); (F.D.)
| | - Joko Kusmoro
- Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (N.S.A.); (B.I.); (J.K.); (F.D.)
| | - Wahyu Safriansyah
- Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (W.S.); (K.F.)
| | - Kindi Farabi
- Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (W.S.); (K.F.)
| | - Dina Oktavia
- Department of Transdisciplinary, Graduate School, Universitas Padjadjaran, Bandung 40132, Indonesia;
| | - Febri Doni
- Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (N.S.A.); (B.I.); (J.K.); (F.D.)
| | - Mia Miranti
- Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (N.S.A.); (B.I.); (J.K.); (F.D.)
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12
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Ghasemi S, Evazalipour M, Peyghanbari N, Zamani E, Bellstedt P, Molaee M, Koohi DE, Yousefbeyk F. Isolation and structure elucidation of the compounds from Teucrium hyrcanicum L. and the investigation of cytotoxicity, antioxidant activity, and protective effect on hydrogen peroxide-induced oxidative stress. BMC Complement Med Ther 2023; 23:447. [PMID: 38087220 PMCID: PMC10714485 DOI: 10.1186/s12906-023-04262-8] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/24/2023] [Accepted: 11/16/2023] [Indexed: 12/18/2023] Open
Abstract
BACKGROUND Teucrium hyrcanicum L. (family Lamiaceae) is widely distributed in the North and Northwest of Iran. It has been used in the form of tea, tonic, and tincture for the treatment of various diseases such as cough, rheumatism, and fever. METHODS In this study, the total phenolic and flavonoid contents, antioxidant and cytotoxic activities of methanol extract and different fractions of T. hyrcanicum were measured. Furthermore, the potential ability of T. hyrcanicum to protect against H2O2-induced oxidative stress was tested on the NIH3T3 cell line. Then, the isolation and structure elucidation of the compounds were performed on the most potent fractions. Finally, the quantification of isolated compounds in methanol extract (ME) was done by the HPLC method. Isolated phytochemicals were assessed for the cytotoxic and antioxidant activities. RESULTS The results indicated that the methanol fraction (MF) had the highest amount of phenolic and flavonoid contents (69.36 mg GAE/g extract and 68.95 mg QE/g extract). The highest radical scavenging activities were observed from MF and ME (IC50 44.32 and 61.12 μg.ml-1, respectively). The best cytotoxicity was obtained by ethyl acetate fraction (EF) against A431 and MCF7 cell lines (IC50 values of 235.4and 326.6 μg.ml-1, respectively). The pretreatment with MF exerts the highest reduction in malondialdehyde (MDA) formation (IC50 2.51 μM, p < 0.001) compared to the H2O2 group (5.77 μM). Also, MF significantly inhibited H2O2-induced Glutathione (GSH) oxidation (p < 0.001). Furthermore, two phenolic compounds, acteoside and quercetin, were isolated and identified in MF and EF, respectively. The IC50 values of acteoside and quercetin in the DPPH assay were 7.19 and 5.56 µg.ml-1, respectively. Both quercetin and acteoside significantly reduced the MDA formation and inhibited GSH oxidation, which was comparable with BHA (as a standard antioxidant) (p < 0.05). Acteoside demonstrated significant cytotoxicity against all tested cell lines (IC50 = 32 to 145 μg.ml-1). The HPLC quantification of isolated compounds revealed that the quantity of acteoside and quercetin in ME were 93.31 and 16.87 μg.mg-1, respectively. CONCLUSION The isolated compounds (quercetin and acteoside) had significant antioxidant activities and revealed a protective effect on H2O2-induced oxidative stress which was comparable with BHA.
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Affiliation(s)
- Saeed Ghasemi
- Department of Medicinal Chemistry, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
| | - Mehdi Evazalipour
- Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
| | - Nastaran Peyghanbari
- Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
| | - Ehsan Zamani
- Department of Pharmacology and Toxicology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
| | - Peter Bellstedt
- Institute of Clinical Chemistry, University of Zurich & University Hospital Zurich, Zurich, Switzerland
| | - Mahan Molaee
- Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
| | - Diba Eghbali Koohi
- Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
| | - Fatemeh Yousefbeyk
- Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.
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Benedec D, Oniga I, Hanganu D, Tiperciuc B, Nistor A, Vlase AM, Vlase L, Pușcaș C, Duma M, Login CC, Niculae M, Silaghi-Dumitrescu R. Stachys Species: Comparative Evaluation of Phenolic Profile and Antimicrobial and Antioxidant Potential. Antibiotics (Basel) 2023; 12:1644. [PMID: 37998846 PMCID: PMC10669438 DOI: 10.3390/antibiotics12111644] [Citation(s) in RCA: 4] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/24/2023] [Revised: 11/14/2023] [Accepted: 11/18/2023] [Indexed: 11/25/2023] Open
Abstract
This study aimed to investigate the polyphenolic composition and antioxidant and antimicrobial potential of six Romanian Stachys species: S. officinalis, S. germanica, S. byzantina, S. sylvatica, S. palustris, and S. recta. The LC-MS/MS method was used to analyze the polyphenolic profile, while the phenolic contents were spectrophotometrically determined. The antioxidant activity was evaluated using the following methods: DPPH, FRAP, nitrite-induced autooxidation of hemoglobin, inhibition of cytochrome c-catalyzed lipid peroxidation, and electron paramagnetic resonance spectroscopy. The in vitro antimicrobial properties were assessed using agar-well diffusion, broth microdilution, and antibiofilm assays. Fifteen polyphenols were identified using LC-MS and chlorogenic acid was the major component in all the samples (1131.8-6761.4 μg/g). S. germanica, S. palustris, and S. byzantina extracts each displayed an intense antiradical action in relation to high contents of TPC (6.40 mg GAE/mL), flavonoids (3.90 mg RE/mL), and caffeic acid derivatives (0.89 mg CAE/mL). In vitro antimicrobial and antibiofilm properties were exhibited towards Candida albicans, Gram-positive and Gram-negative strains, with the most intense efficacy recorded for S. germanica and S. byzantina when tested against S. aureus. These results highlighted Stachys extracts as rich sources of bioactive compounds with promising antioxidant and antimicrobial efficacies and important perspectives for developing phytopharmaceuticals.
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Affiliation(s)
- Daniela Benedec
- Department of Pharmacognosy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 I. Creanga Street, 400010 Cluj-Napoca, Romania; (D.B.); (I.O.); (A.N.)
| | - Ilioara Oniga
- Department of Pharmacognosy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 I. Creanga Street, 400010 Cluj-Napoca, Romania; (D.B.); (I.O.); (A.N.)
| | - Daniela Hanganu
- Department of Pharmacognosy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 I. Creanga Street, 400010 Cluj-Napoca, Romania; (D.B.); (I.O.); (A.N.)
| | - Brîndușa Tiperciuc
- Department of Pharmaceutical Chemistry, “Iuliu Hațieganu” University of Medicine and Pharmacy, 41 V. Babeş Street, 400012 Cluj-Napoca, Romania;
| | - Adriana Nistor
- Department of Pharmacognosy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 I. Creanga Street, 400010 Cluj-Napoca, Romania; (D.B.); (I.O.); (A.N.)
| | - Ana-Maria Vlase
- Department of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 I. Creanga Street, 400010 Cluj-Napoca, Romania;
| | - Laurian Vlase
- Department of Pharmaceutical Technology and Biopharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 41 V. Babeş Street, 400012 Cluj-Napoca, Romania;
| | - Cristina Pușcaș
- Department of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 A. Janos Street, 400028 Cluj-Napoca, Romania; (C.P.); (R.S.-D.)
| | - Mihaela Duma
- State Animal Health and Safety Veterinary Laboratory, 1 Piata Marasti Street, 400609 Cluj-Napoca, Romania
| | - Cristian Cezar Login
- Department of Physiology, “Iuliu Hațieganu” University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania;
| | - Mihaela Niculae
- Department of Clinical Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania;
| | - Radu Silaghi-Dumitrescu
- Department of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 A. Janos Street, 400028 Cluj-Napoca, Romania; (C.P.); (R.S.-D.)
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Ameen F. Taxonomic studies based on leaf epidermal microanatomy using high-resolution microscopy in Lamiaceous species and their antimicrobial effects. Microsc Res Tech 2023; 86:1484-1495. [PMID: 37477095 DOI: 10.1002/jemt.24371] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/16/2023] [Revised: 05/22/2023] [Accepted: 06/05/2023] [Indexed: 07/22/2023]
Abstract
The knowledge of essential oil antimicrobial activity of Lamiaceous species is assessed to describe its effects. The comprehensive foliar trichomes and stomatal morphology of the leaves of essential oil-bearing plants from the family Lamiaceae revealed diverse antimicrobial properties. The aim of this study was to investigate the foliar anatomical traits of 19 Lamiaceous taxa belonging to different tribes using light and scanning electron microscopy to correctly diagnose the species. The microanatomy of the foliar epidermis, trichomes diversity, and the stomatal apertural complex was visualized. Quantitative measurements were noted to describe the variations and the qualitative aspects for example, polygonal shape epidermal cells were examined. The stomatal aperture of four types and trichomes appendages both non-glandular and glandular was identified. Significant variation was found in both quantitative and qualitative traits, including unique ornamentation on the trichomes. The taxonomic key was constructed for accurate identification using qualitative morpho-structural traits. The outcomes of this research explored taxonomically to accurately identify the Lamiaceous species using anatomical characters. This study will provide provides the ecological adaptation linked to evolutionary traits of leaf surfaces that evolve with time to adapt the harsh environmental conditions. RESEARCH HIGHLIGHTS: Investigated foliar anatomical traits of 19 Lamiaceous species The anatomy and antimicrobial activity of essential oil yielding Lamiaceae species. SEM revealed diverse aspects including peculiar sculptured trichomes Microscopic identification of different stomatal complex.
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Affiliation(s)
- Fuad Ameen
- Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia
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15
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Mandura Jarić A, Čikoš A, Pocrnić M, Aladić K, Jokić S, Šeremet D, Vojvodić Cebin A, Komes D. Teucrium montanum L.-Unrecognized Source of Phenylethanoid Glycosides: Green Extraction Approach and Elucidation of Phenolic Compounds via NMR and UHPLC-HR MS/MS. Antioxidants (Basel) 2023; 12:1903. [PMID: 38001756 PMCID: PMC10669637 DOI: 10.3390/antiox12111903] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/26/2023] [Revised: 10/17/2023] [Accepted: 10/20/2023] [Indexed: 11/26/2023] Open
Abstract
Health-oriented preferences, a demand for innovative food concepts, and technological advances have greatly influenced changes in the food industry and led to remarkable development of the functional food market. Incorporating herbal extracts as a rich source of bioactive compounds (BC) could be an effective solution to meet the high demand of consumers in terms of expanding the high-quality range of functional foods. The aim of this study is the valorization of the bioactive potential of T. montanum L., an understudied Mediterranean plant species, and the in-depth elucidation of a polyphenolic profile with a UHPLC-HR MS/MS and NMR analysis. The total phenolic content (TPC) and antioxidant capacity (AC) were determined on heat-assisted (HAE), microwave-assisted (MAE) and subcritical water (SWE) extracts. In terms of antioxidant capacity, SWE extracts showed the most notable potential (ABTS: 0.402-0.547 mmol eq Trolox g-1 dw, DPPH: 0.336-0.427 mmol eq Trolox g-1 dw). 12 phenolic compounds were identified in the samples of T. montanum from six microlocations in Croatia, including nine phenylethanoid glycosides (PGs) with total yields of 30.36-68.06 mg g-1 dw and 25.88-58.88 mg g-1 dw in HAE and MAE extracts, respectively. Echinacoside, teupolioside, stachysoside A, and poliumoside were the most abundant compounds HAE and MAE extracts, making T. montanum an emerging source of PGs.
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Affiliation(s)
- Ana Mandura Jarić
- Department of Food Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotii St. 6, 10000 Zagreb, Croatia; (A.M.J.); (D.Š.); (A.V.C.)
| | - Ana Čikoš
- NMR Centre, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, Croatia
| | - Marijana Pocrnić
- Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia;
| | - Krunoslav Aladić
- Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, 31000 Osijek, Croatia; (K.A.); (S.J.)
| | - Stela Jokić
- Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, 31000 Osijek, Croatia; (K.A.); (S.J.)
| | - Danijela Šeremet
- Department of Food Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotii St. 6, 10000 Zagreb, Croatia; (A.M.J.); (D.Š.); (A.V.C.)
| | - Aleksandra Vojvodić Cebin
- Department of Food Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotii St. 6, 10000 Zagreb, Croatia; (A.M.J.); (D.Š.); (A.V.C.)
| | - Draženka Komes
- Department of Food Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotii St. 6, 10000 Zagreb, Croatia; (A.M.J.); (D.Š.); (A.V.C.)
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Bini KKN, Kobenan KC, Kouakou M, Kouadio IS, Zengin G, Jekő J, Cziáky Z, Danho M, Ochou OG. Phytochemical profiling, antioxidant activities, enzymatic activities and insecticidal potential of aqueous extracts of four plants on the larvae of Helicoverpa armigera (Lepidoptera: Noctuidae), the main pest of cotton plant in Ivory Coast. ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 2023:e22017. [PMID: 37185885 DOI: 10.1002/arch.22017] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/02/2023] [Revised: 04/02/2023] [Accepted: 04/10/2023] [Indexed: 05/17/2023]
Abstract
In the context of climate change, the Ivorian cotton industry is facing with the loss of sensitivity of pests (Helicoverpa armigera) and the appearance of new so-called emerging insects. Faced with this situation, cotton producers tend to use insecticide products in high doses, in excess of the norm. However, the misuse of chemical products poses many health risks. Therefore, to limit the use of chemicals, aqueous extracts of local plants with insecticidal properties were examined in the laboratory and in the field. Four local plant species were selected [Anacardium occidentale (Anarcardier); Azadirachta indica (Neem); Hyptis suaveolens (Hyptis) and Tephrosia vogelii (Tephrosia)]. After determining the chemical profiles of the four extracts by high performance liquid chromatography (HPLC)-mass spectrometry, their inhibitory activities were assessed in cholinesterase and tyrosinase. The sensitivity of Helicoverpa armigera larvae was evaluated by ingesting the aqueous extracts at several concentrations ranging from 2% to 64% in an artificial nutrient substrate. Then, the mortality rates of the larvae during 72 h were evaluated and the lethal concentrations were determined. The results of chemical analyses (HPLC) showed that the richest aqueous extract in phytochemicals with 54 elements detected was that of cashew (A. occidentale). T. vogelii, A. indica and H. suaveolens presented 44, 45, and 39 chemical compounds, respectively. In addition, the total phenolic content was higher in A. occidentale (110.67 mg gallic acid equivalents/g) followed by A. indica (42.43 mg gallic acid equivalents/g). The highest antioxidant ability was observed with the aqueous extract of cashew (A. occidentale). Anti-enzymatic activities such as acetylcholinesterase, butyrylcholinesterase and tyrosinase inhibition were most pronounced in A. occidentale (2.35 ± 0.02 mg galanthamine equivalent/g, 3.77 ± 0.01 mg galanthamine equivalent/g and 71.28 ± 0.07 mg kojic acid equivalent/g, respectively). The most toxic aqueous extract for H. armigera larvae was that of cashew with a lethal concentration LC50 = 11.68%. Moreover, the principal component analysis performed showed that the insecticidal activity is strongly correlated with the antioxidant and enzymatic activities of the aqueous extracts. Then, the hierarchical ascending classification showed cashew as the best plant. For the sustainability of cotton production, it would be necessary to limit the use of chemical-synthetic insecticides through the use of plant extracts, especially from cashew leaves.
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Affiliation(s)
- Kouadio Kra Norbert Bini
- Laboratory of Entomology, Cotton Research Station, National Center of Agronomic Research (CNRA), Adiopodoumé, Cote d'Ivoire
| | - Koffi Christophe Kobenan
- Laboratory of Entomology, Cotton Research Station, National Center of Agronomic Research (CNRA), Adiopodoumé, Cote d'Ivoire
| | - Malanno Kouakou
- Laboratory of Entomology, Cotton Research Station, National Center of Agronomic Research (CNRA), Adiopodoumé, Cote d'Ivoire
| | - Ibrahime Sinan Kouadio
- Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Campus, Konya, Turkey
| | - Gokhan Zengin
- Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Campus, Konya, Turkey
| | - József Jekő
- Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyíregyháza, Hungary
| | - Zoltán Cziáky
- Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyíregyháza, Hungary
| | - Mathias Danho
- Department of Training and Research Agriculture and Animal Resources, National Polytechnic Institute Félix Houphouët-Boigny (INPHB), Yamoussoukro, Ivory Coast
| | - Ochou Germain Ochou
- Laboratory of Entomology, Cotton Research Station, National Center of Agronomic Research (CNRA), Adiopodoumé, Cote d'Ivoire
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Sarfaraz D, Rahimmalek M, Sabzalian MR, Gharibi S, Matkowski A, Szumny A. Essential Oil Composition and Antioxidant Activity of Oregano and Marjoram as Affected by Different Light-Emitting Diodes. Molecules 2023; 28:molecules28093714. [PMID: 37175125 PMCID: PMC10180255 DOI: 10.3390/molecules28093714] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/07/2023] [Revised: 04/13/2023] [Accepted: 04/22/2023] [Indexed: 05/15/2023] Open
Abstract
Oregano and marjoram are important aromatic spices in the food industry, as well as medicinal plants with remarkable antioxidant properties. Despite their popularity, little is known about treatments that would influence the antioxidant capacity of essential oils. In this study, different spectra of LED light, namely blue, red, white, blue-red, and natural ambient light as a control, were applied to assess the essential oil content, composition, flavonoid, phenolic, and antioxidant capacity of oregano and marjoram. GC-MS analysis revealed thymol, terpinen-4-ol, sabinene, linalool, p-cymene, and γ-terpinene as the main compounds. In oregano, the thymol content ranged from 11.91% to 48.26%, while in marjoram it varied from 17.47% to 35.06% in different samples. In oregano and marjoram, the highest phenolic contents were in blue (61.26 mg of tannic acid E/g of DW) and in white (65.18 mg of TAE/g of DW) light, respectively, while blue-red illumination caused the highest increase in total flavonoids. The antioxidant activity of oregano and marjoram extract was evaluated using two food model systems, including DPPH and β-carotene bleaching. The highest antioxidant capacity was obtained in control light in oregano and blue-red light in marjoram. The results provide information on how to improve the desired essential oil profile and antioxidant capacity of extracts for industrial producers.
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Affiliation(s)
- Danial Sarfaraz
- Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran
| | - Mehdi Rahimmalek
- Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran
- Department of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, Poland
| | - Mohammad R Sabzalian
- Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran
| | - Shima Gharibi
- Core Research Facilities (CRF), Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran
| | - Adam Matkowski
- Department of Pharmaceutical Biology and Biotechnology, Botanical Garden of Medicinal Plants, Wroclaw Medical University, 50-367 Wrocław, Poland
| | - Antoni Szumny
- Department of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, Poland
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Ahmadian S, Kenari RE, Amiri ZR, Sohbatzadeh F, Khodaparast MHH. Effect of ultrasound-assisted cold plasma pretreatment on cell wall polysaccharides distribution and extraction of phenolic compounds from hyssop (Hyssopus officinalis L.). Int J Biol Macromol 2023; 233:123557. [PMID: 36740126 DOI: 10.1016/j.ijbiomac.2023.123557] [Citation(s) in RCA: 12] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/08/2022] [Revised: 01/22/2023] [Accepted: 02/01/2023] [Indexed: 02/05/2023]
Abstract
Hyssopus officinalis L (Hyssop) is a good source of phenolic compounds. However, conventional methods for extraction of these compounds typically take a long time and have relatively low recovery rates. This study focused on cold atmospheric plasma (CAP) pretreatment and investigated its effects on the ultrasound-assisted extraction (UAE) of phenolic compounds from hyssop. Hyssop was treated at dielectric barrier discharge plasma with air and nitrogen gases for 5, 10, and 15 min. Optical emission spectroscopy was used to evaluate present active species in the plasma. The water contact angle changes, cell wall polysaccharides distribution, and structural variations of the treated samples were determined after treatment. Antioxidant activity and total phenolic contents (TPC) of the extracts were also evaluated. The results showed that CAP treatment reduced the contact angle making surface more hydrophilic. Compared with hyssop, overall no significant changes in the basic structure of all treated samples or the formation of new functional groups were recognized. In addition, CAP pretreatment before UAE increased the antioxidant activity of extracts according to the FRAP assay than the un-pretreated sample and conventional solvent extraction method. Also, TPC increased in samples treated with nitrogen plasma.
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Affiliation(s)
- Soheila Ahmadian
- Department of Food Science and Technology, Faculty of Agricultural Engineering, Sari Agricultural Sciences and Natural Resources University, Km 9 Farah Abad Road, Sari, Iran
| | - Reza Esmaeilzadeh Kenari
- Department of Food Science and Technology, Faculty of Agricultural Engineering, Sari Agricultural Sciences and Natural Resources University, Km 9 Farah Abad Road, Sari, Iran.
| | - Zeynab Raftani Amiri
- Department of Food Science and Technology, Faculty of Agricultural Engineering, Sari Agricultural Sciences and Natural Resources University, Km 9 Farah Abad Road, Sari, Iran
| | - Farshad Sohbatzadeh
- Department of Atomic and Molecular Physics, Faculty of Science, University of Mazandaran, Babolsar, Iran
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Fernandez-Soto P, Celi D, Tejera E, Alvarez-Suarez JM, Machado A. Cinnamomum sp. and Pelargonium odoratissimum as the Main Contributors to the Antibacterial Activity of the Medicinal Drink Horchata: A Study Based on the Antibacterial and Chemical Analysis of 21 Plants. Molecules 2023; 28:693. [PMID: 36677749 PMCID: PMC9862262 DOI: 10.3390/molecules28020693] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/10/2022] [Revised: 12/28/2022] [Accepted: 01/04/2023] [Indexed: 01/13/2023] Open
Abstract
Horchata, a herbal infusion drink from Ecuador containing a mixture of medicinal plants, has been reported to exhibit anti-inflammatory, analgesic, diuretic, and antioxidant activity. The antibacterial activity of each of the plants contained in the horchata mixture has not been fully evaluated. Thus, in this study, we analysed the antibacterial activity of 21 plants used in horchata, collected from the Ecuadorian Andes region, against bacterial strains of clinical importance. The methanolic extract of Cinnamomum sp. showed minimal inhibitory concentration (MIC) values of 250 µg/mL against Staphylococcus aureus ATCC25923 and Methicillin-resistant S. aureus (MRSA), while Pelargonium odoratissimum exhibited a MIC value of 500 µg/mL towards S. aureus ATCC25923. The high-performance liquid chromatography-diode array detector-tandem mass spectrometry (HPLC-DAD-MS/MS) analyses identified in Cinnamomum sp. epicatechin tannins, cinnamaldehyde, and prehelminthosporol molecules, whereas in P. odoratissimum, gallocatechin and epigallocatechin tannins, some flavonoids, and gallic acid and derivatives were identified. Finally, Cinnamomum sp. and P. odoratissimum showed partial inhibition of biofilm formation of S. aureus ATCC25923 and MRSA. Overall, our findings revealed which of the plants used in horchata are responsible for the antibacterial activity attributed to this herbal drink and exhibit the potential for Cinnamomum sp. and P. odoratissimum secondary metabolites to be explored as scaffolds in drug development.
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Affiliation(s)
- Paulina Fernandez-Soto
- Facultad de Ciencias de la Salud, Carrera de Enfermería, Grupo de Bio-Quimioinformática, Universidad de Las Américas (UDLA), Quito 170125, Ecuador
| | - Diana Celi
- Facultad de Ingeniería y Ciencias Aplicadas, Carrera de Ingeniería Agroindustrial, Universidad de Las Américas (UDLA), Quito 170125, Ecuador
| | - Eduardo Tejera
- Facultad de Ingeniería y Ciencias Agropecuarias Aplicadas, Grupo de Bio-Quimioinformática, Universidad de Las Américas (UDLA), Quito 170125, Ecuador
| | - José Miguel Alvarez-Suarez
- Colegio de Ciencias e Ingenierías, Departamento de Ingeniería en Alimentos, Universidad San Francisco de Quito (USFQ), Quito 170901, Ecuador
| | - António Machado
- Colegio de Ciencias Biológicas y Ambientales (COCIBA), Instituto de Microbiología, Laboratorio de Bacteriología, Universidad San Francisco de Quito (USFQ), Quito 170901, Ecuador
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Ghasempour M, Hosseini M, Soltani-Zangbar MS, Motavalli R, Aghebati-Maleki L, Dolati S, Mehdizadeh A, Yousefi M, Ahmadian Heris J. The impact of Hyssop (Hyssopus officinalis) extract on activation of endosomal toll like receptors and their downstream signaling pathways. BMC Res Notes 2022; 15:366. [PMID: 36503515 PMCID: PMC9742021 DOI: 10.1186/s13104-022-06253-3] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/31/2022] [Accepted: 11/23/2022] [Indexed: 12/14/2022] Open
Abstract
OBJECTIVES From the ancient, medicinal benefits of Hyssop (Hyssopus officinalis L.) have been implicated for respiratory and digestive diseases despite the effects of Hyssop on viral infections have not been mechanistically investigated. In this study, we examined whether the Hyssop extract activated anti-viral innate immunity, as a sentinel for immune system, through activation of endosomal TLRs recognizing nucleic acids and their downstream signaling. The Hyssop herb extracts was prepared and co-cultured with healthy individual's peripheral blood mononuclear cells (PBMCs). After viability assay, gene expression levels of TLR3,7,8,9, as well as MyD88 and NF-κB, were evaluated in treated PBMCs using Real-time PCR. Next, the secretion level of immune related cytokines was quantified via ELISA. RESULTS Post 24 h, 40 µg/ml of the extract significantly inhibited the viability of less than 50% of cells compared to the control and had a maximum effect on cellular function. The Hyssop-treated PBMCs demonstrated an elevated expression of endosomal TLRs genes, as well as MyD88 and NF-κB. Moreover, the release of INF-α and β notably enhanced in cell culture supernatant, while the content of inflammatory cytokines remarkably diminished (P < 0.05). The Hyssop extract was capable of inducing antiviral innate immune responses so can be promising in antiviral drug strategies.
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Affiliation(s)
- Masoumeh Ghasempour
- Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
- Department of Allergy and Clinical Immunology, Pediatric Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Maryam Hosseini
- Trauma Research Center, Shahid Rajaee (Emtiaz) Trauma Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
| | - Mohammad Sadegh Soltani-Zangbar
- Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
- Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Roza Motavalli
- Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
- Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
| | | | - Sanam Dolati
- Physical Medicine and Rehabilitation Research Center, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Amir Mehdizadeh
- Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Mehdi Yousefi
- Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
- Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Javad Ahmadian Heris
- Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
- Department of Allergy and Clinical Immunology, Pediatric Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.
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Antioxidant Properties and Kidney Cell Protection by the Extracts of Curcuma longa, Artemisia princeps, Salicornia herbacea, and Schisandra chinesis. FERMENTATION-BASEL 2022. [DOI: 10.3390/fermentation8120702] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/12/2022]
Abstract
This study evaluated the antioxidant properties and kidney cell protection of medicinal plants as natural medicaments. A total of four medicinal plants including turmeric (TM), gangwha mugwort (GM), glasswort (GW), and omija (OM) were selected and fermented. Hot water extracts (HWE) and ethanol extracts (EE) of the plants were prepared before and after fermentation and tested in experiments in vitro and in vivo. Total polyphenol contents (TPC) and total flavonoid content (TFC) of GM were the highest among them. The TPC based HWE decreased after fermentation except OM; in contrast, TFC from HWE increased. The DPPH radical scavenging activity and ABTS value from HWE increased after fermentation, especially OM, which showed significant differences, while DPPH and ABTS from EE were decreased. The cell viability was not changed after addition of these plants extracts below 50 μg/mL; however, TM from HWE significantly decreased. The protective effect on kidney cells against cisplatin showed a 60% range of cell viability in each plant extract. In the in vivo experiment, the protective effect on kidney cells by the supplemented plant extracts was demonstrated by the serum creatinine and BUN level. During experimental periods, the serum creatinine and BUN level of GW and GM-treated mice decreased with significant differences compared to the adenine control group. As a result, these plant extracts had no cytotoxicity and maintained a protective effect as well as antioxidant activity. These results suggest that plants such as gangwha mugwort (GM) and glasswort (GW) may be good extracts for kidney cell protection and antioxidant agents.
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Balciunaitiene A, Puzeryte V, Radenkovs V, Krasnova I, Memvanga PB, Viskelis P, Streimikyte P, Viskelis J. Sustainable-Green Synthesis of Silver Nanoparticles Using Aqueous Hyssopus officinalis and Calendula officinalis Extracts and Their Antioxidant and Antibacterial Activities. MOLECULES (BASEL, SWITZERLAND) 2022; 27:molecules27227700. [PMID: 36431804 PMCID: PMC9696917 DOI: 10.3390/molecules27227700] [Citation(s) in RCA: 12] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 09/30/2022] [Revised: 11/04/2022] [Accepted: 11/07/2022] [Indexed: 11/11/2022]
Abstract
Silver nanoparticles (AgNPs) biosynthesized using aqueous medical plant extracts as reducing and capping agents show multiple applicability for bacterial problems. The aim of this study was to expand the boundaries on AgNPs using a novel, low-toxicity, and cost-effective alternative and green approach to the biosynthesis of metallic NPs using Calendula officinalis (Calendula) and Hyssopus officinalis (Hyssopus) aqueous extracts. The formation of AgNPs was confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS) techniques. The effectiveness of biosynthesized AgNPs in quenching free radicals and inhibiting the growth of Gram-positive and Gram-negative microorganisms was supported by in vitro antioxidant activity assay methods and using the Kirby-Bauer disk diffusion susceptibility test, respectively. The elucidated antimicrobial and antioxidative activities of medical plant extracts were compared with data from the engineered biosynthetic AgNPs. The antimicrobial effect of engineered AgNPs against selected test cultures was found to be substantially stronger than for plant extracts used for their synthesis. The analysis of AgNPs by TEM revealed the presence of spherical-shaped nano-objects. The size distribution of AgNPs was found to be plant-type-dependent. The smaller AgNPs were obtained with Hyssopus extract (with a size range of 16.8 ± 5.8 nm compared to 35.7 ± 4.8 nm from Calendula AgNPs). The AgNPs' presumably inherited biological functions of Hyssopus and Calendula medical plants can provide a platform to combat pathogenic bacteria in the era of multi-drug resistance.
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Affiliation(s)
- Aiste Balciunaitiene
- Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, 54333 Kaunas, Lithuania
- Correspondence: (A.B.); (P.V.); Tel.: +370-682-13568 (P.V.)
| | - Viktorija Puzeryte
- Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, 54333 Kaunas, Lithuania
| | - Vitalijs Radenkovs
- Processing and Biochemistry Department, Institute of Horticulture, LV-3701 Dobele, Latvia
- Research Laboratory of Biotechnology, Division of Smart Technologies, Latvia University of Life Sciences and Technologies, LV-3004 Jelgava, Latvia
| | - Inta Krasnova
- Processing and Biochemistry Department, Institute of Horticulture, LV-3701 Dobele, Latvia
| | - Patrick B. Memvanga
- Laboratory of Pharmaceutics and Phytopharmaceutical Drug Development, Faculty of Pharmaceutical Sciences, University of Kinshasa, B.P. 212, Kinshasa 012, Congo
- Department of Pharmacy, Faculty of Medecine and Pharmacy, University of Kisangani, B.P. 212, Kisangani 012, Congo
- Department of Pharmacy, Faculty of Pharmaceutical Sciences and Public Health, Official University of Bukavu, Bukavu B.P. 570, Congo
- Centre de Recherche et d’Innovation Technologique en Environnement et en Sciences de la Santé (CRITESS), University of Kinshasa, B.P. 212, Kinshasa 012, Congo
| | - Pranas Viskelis
- Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, 54333 Kaunas, Lithuania
- Correspondence: (A.B.); (P.V.); Tel.: +370-682-13568 (P.V.)
| | - Paulina Streimikyte
- Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, 54333 Kaunas, Lithuania
| | - Jonas Viskelis
- Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, 54333 Kaunas, Lithuania
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Arbab S, Ullah H, Bano I, Li K, Ul Hassan I, Wang W, Qadeer A, Zhang J. Evaluation of in vitro antibacterial effect of essential oil and some herbal plant extract used against mastitis pathogens. Vet Med Sci 2022; 8:2655-2661. [DOI: 10.1002/vms3.959] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/07/2022] Open
Affiliation(s)
- Safia Arbab
- Key Laboratory of Veterinary Pharmaceutical Development Ministry of Agriculture Lanzhou China
- Key Laboratory of New Animal Drug Project of Gansu Province Lanzhou China
- Lanzhou Institute of Husbandry and Pharmaceutical Sciences Chinese Academy of Agricultural Sciences Beijing China
| | - Hanif Ullah
- West China School of Nursing/ West China Hospital Sichuan University Chengdu China
| | - Iqra Bano
- Faculty of Bioscience Department of Physiology and Biochemistry, Shaheed Benazir Bhutto University of Veterinary & Animal Sciences Sakrand Pakistan
| | - Ka Li
- West China School of Nursing/ West China Hospital Sichuan University Chengdu China
| | - Inam Ul Hassan
- Department of Microbiology Hazara University Manshera Khyber Pakhtunkhwa Pakistan
| | - Weiwei Wang
- Key Laboratory of Veterinary Pharmaceutical Development Ministry of Agriculture Lanzhou China
- Key Laboratory of New Animal Drug Project of Gansu Province Lanzhou China
- Lanzhou Institute of Husbandry and Pharmaceutical Sciences Chinese Academy of Agricultural Sciences Beijing China
| | - Abdul Qadeer
- Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences Shanghai China
| | - Jiyu Zhang
- Key Laboratory of Veterinary Pharmaceutical Development Ministry of Agriculture Lanzhou China
- Key Laboratory of New Animal Drug Project of Gansu Province Lanzhou China
- Lanzhou Institute of Husbandry and Pharmaceutical Sciences Chinese Academy of Agricultural Sciences Beijing China
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Khaksar S, Kiarostami K, Alinaghi S. The Effects of Methanol Extracts of Hyssopus officinalis on Model of Induced Glioblastoma Multiforme (GBM) in Rats. J Mol Neurosci 2022; 72:2045-2066. [PMID: 35963984 DOI: 10.1007/s12031-022-02058-y] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/24/2022] [Accepted: 08/04/2022] [Indexed: 11/27/2022]
Abstract
Given the complexity of pathophysiological processes of brain tumors, ineffective therapies, and high mortality rate, new therapeutic options with less toxicity are necessary. Hyssopus officinalis (hyssop) is an aromatic plant with important biological activities. The aim of this study is to assess the anti-cancer effect of hyssop extract on damages of glioblastoma multiforme. In this study, total flavonoids, phenolic content, and quantification of phenolic compound of hyssop extracts were analyzed. In vitro antioxidant properties of hyssop extract were also examined. In addition, cell viability, apoptosis, and cell cycle were evaluated in C6 glioma cell culture. In vivo, the rats were divided randomly into four main groups: intact, control, vehicle, and treatment groups. 1 × 106 C6 rat glioma cells were implanted into the right caudate nucleus of the rat's brain. The treatment group received the methanol extract of hyssop (100 mg/kg) for 7 days. Evolution of locomotor activity, tumor volume, survival rate, activities of antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)), vascular endothelial growth factor (VEGF) expression, TUNEL-positive cells, p53 and p21 mRNA expression, and histological alterations were performed. The results showed that the methanol extract of hyssop increased the apoptosis and reduced the cell division of C6 glioma cells in cell culture. Moreover, methanol extract decreased the tumor volume and prolonged survival. Also, the activity of SOD and CAT enzymes was reduced in tumor tissue and enhanced in surrounding tissue. TUNEL-positive cells were increased in methanol extract of hyssop group. The expression of p53 and p21 mRNA was upregulated in the treatment group. Moreover, the histological analysis indicated a considerable decrease in invasion of tumor cells and inflammation in the hyssop-treated rats. According to the achieved results, it can be stated that hyssop has sufficient potential to inhibit damage of brain tumors, at least in part, by affecting the oxidative stress and cell proliferation pathways.
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Affiliation(s)
- Sepideh Khaksar
- Department of Plant Sciences, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
| | - Khadijeh Kiarostami
- Department of Plant Sciences, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
| | - Shahrzad Alinaghi
- Department of Plant Sciences, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
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Mićović T, Katanić Stanković JS, Bauer R, Nöst X, Marković Z, Milenković D, Jakovljević V, Tomović M, Bradić J, Stešević D, Stojanović D, Maksimović Z. In vitro, in vivo and in silico evaluation of the anti-inflammatory potential of Hyssopus officinalis L. subsp. aristatus (Godr.) Nyman (Lamiaceae). JOURNAL OF ETHNOPHARMACOLOGY 2022; 293:115201. [PMID: 35358622 DOI: 10.1016/j.jep.2022.115201] [Citation(s) in RCA: 9] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/24/2021] [Revised: 03/07/2022] [Accepted: 03/11/2022] [Indexed: 06/14/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Medicinal properties of hyssop have been used in traditional medicine since ancient times, inter alia, in diseases/conditions with an inherent inflammatory process. AIM OF THE STUDY Accordingly, the aim of this study was to investigate the anti-inflammatory properties of hyssop herb preparations (essential oil and methanol extracts) in vivo, in vitro and in silico. MATERIALS AND METHODS For in vitro testing of essential oils and extracts of hyssop herb, the cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) enzyme assays were used. In vivo anti-inflammatory potential of the extracts (at doses of 50, 100 and 200 mg/kg) was assessed using the carrageenan-induced rat paw edema test. Molecular docking and dynamics were used for in silico testing of the inhibitory activity of chlorogenic (CA) and rosmarinic (RA) acids, as the dominant compounds in the tested methanol extracts against COX-1 and COX-2 enzymes. RESULTS Significant inhibitory activity was shown in the COX-2 test regarding extracts (essential oils did not exhibit any significant activity). Namely, all analyzed extracts, at a concentration of 20 μg/mL, showed a percentage of inhibition of COX-2 enzyme (54.04-63.04%), which did not indicate a statistically significant difference from the positive control of celecoxib (61.60%) at a concentration of 8.8 μM. In vivo testing showed that all methanol extracts of hyssop herb, at the highest test dose of 200 mg/kg in the third and fourth hours, after carrageenan administration, exhibited a statistically significant (p < 0.05) inhibitory effect on the increase in rat paw edema in relation to control. This activity is comparable or higher in relation to the reference substance, indomethacin, at a concentration of 8 mg/kg. The preliminary in silico results suggest that investigated compounds (RA and CA) showed better inhibitory activity against COX-1 and COX-2 than standard non-steroidal anti-inflammatory drug (NSAID), ibuprofen, as evident from the free binding energy (ΔGbind in kJ mol-1). The binding energies of the docked compounds to COX-1 and -2 were found to be in the range between -47.4 and -49.2 kJ mol-1. Ibuprofen, as the one NSAID, for the same receptors targets, showed remarkably higher binding energy (ΔGbind = -31.3 kJ mol-1 to COX-1, and ΔGbind = -30.9 kJ mol-1 to COX-2). CONCLUSION The results obtained not only support the traditional use of hyssop herb in inflammatory conditions in folk medicine, but also open the door to and the need for further in vivo testing of extracts in order to examine the molecular mechanism of anti-inflammatory activity in living systems and possibly develop a new anti-inflammatory drug or supplement.
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Affiliation(s)
- Tijana Mićović
- Institute for Medicines and Medical Devices of Montenegro, Bulevar Ivana Crnojevića 64a, 81000, Podgorica, Montenegro
| | - Jelena S Katanić Stanković
- Institute for Information Technologies Kragujevac, Department of Science, University of Kragujevac, Jovana Cvijića bb, 34000, Kragujevac, Serbia.
| | - Rudolf Bauer
- Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Beethovenstrasse 8, 8010, Graz, Austria
| | - Xuehong Nöst
- Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Beethovenstrasse 8, 8010, Graz, Austria
| | - Zoran Marković
- Institute for Information Technologies Kragujevac, Department of Science, University of Kragujevac, Jovana Cvijića bb, 34000, Kragujevac, Serbia
| | - Dejan Milenković
- Institute for Information Technologies Kragujevac, Department of Science, University of Kragujevac, Jovana Cvijića bb, 34000, Kragujevac, Serbia
| | - Vladimir Jakovljević
- Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000, Kragujevac, Serbia; Department of Human Pathology, First Moscow State Medical University I. M. Sechenov, Trubetskaya street 8, str. 2, 119991, Moscow, Russia
| | - Marina Tomović
- Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000, Kragujevac, Serbia
| | - Jovana Bradić
- Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000, Kragujevac, Serbia
| | - Danijela Stešević
- Faculty of Natural Sciences and Mathematics, University of Montenegro, Džordža Vašingtona bb, 81000, Podgorica, Montenegro
| | - Danilo Stojanović
- Department of Botany, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000, Belgrade, Serbia
| | - Zoran Maksimović
- Department of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000, Belgrade, Serbia.
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Foliar Spraying with Potassium Bicarbonate Reduces the Negative Impact of Drought Stress on Sweet Basil (Ocimum basilicum L.). PLANTS 2022; 11:plants11131716. [PMID: 35807668 PMCID: PMC9268819 DOI: 10.3390/plants11131716] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 05/05/2022] [Revised: 06/13/2022] [Accepted: 06/24/2022] [Indexed: 11/17/2022]
Abstract
In recent years, special attention has been paid to climate change assessment and research into our changing environment. The greatest economic losses worldwide are due to the negative effects of drought stress and extreme temperature on the plants’ morphological, physiological, and biochemical properties which limit crop growth and productivity. Sweet basil (Ocimum basilicum L.) is one of the most popular plants widely grown around the world as a spice, as well a medicinal, aromatic plant. The seedlings with 5–6 true leaves were divided into two groups, and one group of seedlings was sprayed with 10 mM potassium bicarbonate (KHCO3). Three days after potassium bicarbonate treatment, half of the plants from each group were subjected to a water deficit (drought stress), and the other half were grown under stress-free conditions (well-watered). The present study aimed to evaluate the effect of potassium bicarbonate (KHCO3) on morphophysiological parameters, phenolics content and the antioxidant activity of basil under drought conditions. The application of potassium bicarbonate to drought stressed plants significantly increased the chlorophyll content, fresh and dry weight, phenolics content in the two of tested cultivars, and antioxidant activity, determined by DPPH and ABTS methods. Principal component analysis showed that the first factor was highly and positively related to all the investigated parameters. Hierarchical clustering analysis showed that the first cluster was formed by being well-watered, well-watered and sprayed with potassium bicarbonate, and grown under drought conditions and sprayed with potassium bicarbonate basil cultivars, while the second cluster was formed by all the tested cultivars grown under drought conditions.
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Abdullah FO, Hussain FHS, Sardar AS, Gilardoni G, Thu ZM, Vidari G. Bio-Active Compounds from Teucrium Plants Used in the Traditional Medicine of Kurdistan Region, Iraq. MOLECULES (BASEL, SWITZERLAND) 2022; 27:molecules27103116. [PMID: 35630593 PMCID: PMC9145536 DOI: 10.3390/molecules27103116] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 04/07/2022] [Revised: 04/28/2022] [Accepted: 05/03/2022] [Indexed: 01/11/2023]
Abstract
Herbal medicine is still widely practiced in the Kurdistan Region, Iraq, especially by people living in villages in mountainous regions. Seven taxa belonging to the genus Teucrium (family Lamiaceae) are commonly employed in the Kurdish traditional medicine, especially to treat jaundice, stomachache and abdominal problems. We report, in this paper, a comprehensive account about the chemical structures and bioactivities of most representative specialized metabolites isolated from these plants. These findings indicate that Teucrium plants used in the folk medicine of Iraqi Kurdistan are natural sources of specialized metabolites that are potentially beneficial to human health.
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Affiliation(s)
- Fuad O. Abdullah
- Department of Chemistry, College of Science, Salahaddin University-Erbil, Erbil 44001, Kurdistan Region, Iraq
- Department of Pharmacognosy, Faculty of Pharmacy, Tishk International University, Erbil 44001, Kurdistan Region, Iraq
- Correspondence: (F.O.A.); (G.V.)
| | - Faiq H. S. Hussain
- Department of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil 44001, Kurdistan Region, Iraq;
| | - Abdullah Sh. Sardar
- Department of Biology, College of Education, Salahaddin University-Erbil, Erbil 44001, Kurdistan Region, Iraq;
| | - Gianluca Gilardoni
- Departamento de Química, Universidad Técnica Particular de Loja, Loja 110107, Ecuador;
| | - Zaw Min Thu
- Department of Chemistry, Kalay University, Kalay 03044, Myanmar;
| | - Giovanni Vidari
- Department of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil 44001, Kurdistan Region, Iraq;
- Dipartimento di Chimica, Università di Pavia, 27100 Pavia, Italy
- Correspondence: (F.O.A.); (G.V.)
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Toxicity, Antioxidant Activity, and Phytochemicals of Basil ( Ocimum basilicum L.) Leaves Cultivated in Southern Punjab, Pakistan. Foods 2022; 11:foods11091239. [PMID: 35563962 PMCID: PMC9102432 DOI: 10.3390/foods11091239] [Citation(s) in RCA: 13] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/13/2022] [Revised: 04/14/2022] [Accepted: 04/20/2022] [Indexed: 12/29/2022] Open
Abstract
Basil (Ocimum basilicum L.) is one of the most common aromatic herbs, a rich source of bioactive compounds, and is used extensively to add aroma and flavor to food. The leaves, both in fresh and dried form, are used as a culinary ingredient in different cultures. O. basilicum is also famous for its therapeutic potential and preservation effects. The present study investigated the cytotoxicity of basil at three different growth stages (GS), i.e., GS-1 (58 days of growth), GS-2 (69 days of growth), and GS-3 (93 days of growth) using the brine shrimp lethality assay. The results revealed that cytotoxicity was influenced by GS and the concentration of extracts. Aqueous extracts of basil at a concentration of 10 to 1000 µg/mL did not show notable toxicity. The lowest mortality rate, i.e., 8.9%, was recorded for GS-2 at the highest tested dose of basil extracts. The mortality rate at GS-1, GS-2, and GS-3 was found to be 26.7 ± 3.34%, 8.91 ± 0.10%, and 16.7 ± 0.34%, respectively, at 1000 µg/mL. GS-2 basil powder with the lowest toxicological risk was extracted with different solvents, viz., n-hexane, dichloromethane, ethanol, and water. The highest concentration of plant secondary metabolites including total phenolic acid, flavonoids, and tannin content was observed in ethanol extracts. Ethanol extracts also exhibited the highest antioxidant activity in DPPH, FRAP and H2O2 assays. LC-ESI-MS/MS analysis presented ethanol extracts of basil as a promising source of known health-promoting and therapeutic compounds such as rosmarinic acid, ellagic acid, catechin, liquiritigenin, and umbelliferone. The results suggest basil, a culinary ingredient, as a potential source of bioactive compounds which may offer an array of health promoting and therapeutic properties.
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Beltrán-Noboa A, Proaño-Ojeda J, Guevara M, Gallo B, Berrueta LA, Giampieri F, Perez-Castillo Y, Battino M, Álvarez-Suarez JM, Tejera E. Metabolomic profile and computational analysis for the identification of the potential anti-inflammatory mechanisms of action of the traditional medicinal plants Ocimum basilicum and Ocimum tenuiflorum. Food Chem Toxicol 2022; 164:113039. [PMID: 35461962 DOI: 10.1016/j.fct.2022.113039] [Citation(s) in RCA: 9] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/15/2022] [Revised: 04/09/2022] [Accepted: 04/14/2022] [Indexed: 12/28/2022]
Abstract
Ocimum basilicum and Ocimum tenuiflorum are two basil species widely used medicinally as an anti-inflammatory, antimicrobial and cardioprotective agent. This study focuses on the chemical characterization of the majoritarian compounds of both species and their anti-inflammatory potential. Up to 22 compounds such as various types of salvianolic acids, derivatives of rosmaniric acid and flavones were identified in both plants. The identified compounds were very similar between both plants and are consistent with previous finding in other studies in Portugal and Italy. Based on the identified molecules a consensus target prediction was carried out. Among the main predicted target proteins, we found a high representation of the carbonic anhydrase family (CA2, CA7 and CA12) and several key proteins from the arachidonic pathway (LOX5, PLA2, COX1 and COX2). Both pathways are well related to inflammation. The interaction between the compounds and these targets were explored through molecular docking and molecular dynamics simulation. Our results suggest that some molecules present in both plants can induce an anti-inflammatory response through a non-steroidal mechanism of action connected to the carbon dioxide metabolism.
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Affiliation(s)
- Andrea Beltrán-Noboa
- Grupo de Bioquimioinformática. Universidad de Las Américas, Quito, Ecuador; Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), Bilbao, Spain
| | - John Proaño-Ojeda
- Grupo de Bioquimioinformática. Universidad de Las Américas, Quito, Ecuador; Facultad de Ingeniería y Ciencias Aplicadas. Carrera de Biotecnología, Universidad de Las Américas, Quito, Ecuador
| | - Mabel Guevara
- Grupo de Bioquimioinformática. Universidad de Las Américas, Quito, Ecuador; Grupo de Investigación en Polifenoles. Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca, Spain
| | - Blanca Gallo
- Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), Bilbao, Spain
| | - Luis A Berrueta
- Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), Bilbao, Spain
| | - Francesca Giampieri
- Department of Clinical Sciences, Università Politecnica delle Marche, Ancona, Italy; Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
| | - Yunierkis Perez-Castillo
- Grupo de Bioquimioinformática. Universidad de Las Américas, Quito, Ecuador; Escuela de Ciencias Físicas y Matemáticas. Universidad de Las Américas, Quito, Ecuador
| | - Maurizio Battino
- Department of Clinical Sciences, Università Politecnica delle Marche, Ancona, Italy; International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang, China
| | - José M Álvarez-Suarez
- Ingeniería en Alimentos, Colegio de Ciencias e Ingenierías, Universidad San Francisco de Quito, Quito, Ecuador; King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; Instituto de Investigaciones en Biomedicina iBioMed, Universidad San Francisco de Quito, Quito, Ecuador.
| | - Eduardo Tejera
- Grupo de Bioquimioinformática. Universidad de Las Américas, Quito, Ecuador; Facultad de Ingeniería y Ciencias Aplicadas. Carrera de Biotecnología, Universidad de Las Américas, Quito, Ecuador.
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Othman MS, Khaled AM, Al-Bagawi AH, Fareid MA, Ghany RA, Habotta OA, Abdel Moneim AE. Hepatorenal protective efficacy of flavonoids from Ocimum basilicum extract in diabetic albino rats: A focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities. Biomed Pharmacother 2021; 144:112287. [PMID: 34649220 DOI: 10.1016/j.biopha.2021.112287] [Citation(s) in RCA: 24] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/11/2021] [Revised: 09/18/2021] [Accepted: 10/05/2021] [Indexed: 02/09/2023] Open
Abstract
Plant derived phytochemical therapy is a bright candidate for treatment of diabetes and its associated complications. Ocimum baslicum is used as an anti-diabetic traditional medicine. Hence, the present study investigated the effect of Hail Ocimum extract (HOE) and its total flavonoids (HOETF) against hepatorenal damage in experimental diabetes induced by high-fat diet (HFD) and injection of streptozotocin (STZ) in rats. Diabetic animals were co-treated daily with HOE, HOETF or metformin (MET) as a standard anti-diabetic drug for four weeks. Compared to controls, HFD/STZ-treatment lead to significant increases in fasting blood glucose, insulin and HOMA-IR levels. Furthermore, diabetic rats had elevated hepatic (ALT and ALP) and kidney functions (urea and creatinine) biomarkers together with disturbed lipid profile and decreased PPAR-γ gene expression. Higher levels of hepatic and renal LPO and NO paralleled with lower levels of GSH and activities of antioxidant enzymes (SOD, CAT, GPx and GR) after HFD/STZ treatment. Additionally, noteworthy inflammatory and apoptotic responses were evident in both organs of diabetic rats as witnessed by augmented levels of TNF-α, IL-1b and Bax levels with declined levels of Bcl-2. Moreover, histological examination of hepatic, renal and pancreatic tissues validated the biochemical findings. On contrary, co-treatment of diabetic animals with HOE or HOETF could decrease glucose and insulin levels together with improvement of lipid markers and alleviation of hepatorenal dysfunction, oxidative injury, inflammatory and apoptotic events. Conclusively, HOE or HOETF could be a promising complementary therapeutic option for the management of diabetic hepatorenal complication owing to their antioxidant, anti-inflammatory; anti-apoptotic properties.
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Affiliation(s)
- Mohamed S Othman
- Basic Sciences Department, Deanship of Preparatory Year, University of Ha'il, Hail, Saudi Arabia; Faculty of Biotechnology, October University for Modern Science and Arts (MSA), Giza, Egypt.
| | - Azza M Khaled
- Basic Sciences Department, Deanship of Preparatory Year, University of Ha'il, Hail, Saudi Arabia; National Institute of Oceanography and Fisheries, Cairo, Egypt
| | - Amal H Al-Bagawi
- Chemistry Department, Faculty of Science, University of Ha'il, Hail, Saudi Arabia
| | - Mohamed A Fareid
- Basic Sciences Department, Deanship of Preparatory Year, University of Ha'il, Hail, Saudi Arabia; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
| | - Reda A Ghany
- Basic Sciences Department, Deanship of Preparatory Year, University of Ha'il, Hail, Saudi Arabia; Chemistry Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
| | - Ola A Habotta
- Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
| | - Ahmed E Abdel Moneim
- Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt.
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Discovery of Phenolic Glycoside from Hyssopus cuspidatus Attenuates LPS-Induced Inflammatory Responses by Inhibition of iNOS and COX-2 Expression through Suppression of NF-κB Activation. Int J Mol Sci 2021; 22:ijms222212128. [PMID: 34830006 PMCID: PMC8623068 DOI: 10.3390/ijms222212128] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/24/2021] [Revised: 11/05/2021] [Accepted: 11/06/2021] [Indexed: 11/17/2022] Open
Abstract
It seems quite necessary to obtain effective substances from natural products against inflammatory response (IR) as there are presently clinical problems regarding accompanying side effects and lowered quality of life. This work aimed to investigate the abilities of hyssopuside (HY), a novel phenolic glycoside isolated from Hyssopus cuspidatus (H. cuspidatus), against IR in lipopolysaccharide (LPS)-induced RAW 264.7 cells and mouse peritoneal macrophages. The results indicated that HY could reduce nitric oxide (NO) production and inhibit the production and secretion of pro-inflammatory mediators including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in LPS-stimulated macrophages. Moreover, data from the immunofluorescence study showed that HY suppressed nuclear translocation of nuclear factor-kappa B (NF-κB) upon LPS induction. The Western blot results suggested that HY reversed the LPS-induced degradation of IκB (inhibitor of NF-κB), which is normally required for the activation of NF-κB. Meanwhile, the overexpression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) diminished significantly with the presence of HY in response to LPS stimulation. On the other hand, HY had a negligible impact on the activation of mitogen-activated protein kinase (MAPK) pathways. Moreover, an in silico study of HY against four essential proteins/enzymes revealed that COX-2 was the most efficient enzyme for the interaction, and binding of residues Phe179, Asn351, and Ser424 with HY played crucial roles in the observed activity. The structure analysis indicated the typical characterizations with phenylethanoid glycoside contributed to the anti-inflammatory effects of HY. These results indicated that HY manipulated its anti-inflammatory effects mainly through blocking the NF-κB signal transduction pathways. Collectively, we believe that HY could be a potential alternative phenolic agent for alleviating excessive inflammation in many inflammation-associated diseases.
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Extraction Processes Affect the Composition and Bioavailability of Flavones from Lamiaceae Plants: A Comprehensive Review. Processes (Basel) 2021. [DOI: 10.3390/pr9091675] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/13/2022] Open
Abstract
Lamiaceae plants are a widespread family of herbaceous plants with around 245 plant genera and nearly 22,576 species distributed in the world. Some of the most representative and widely studied Lamiaceae plants belong to the Ocimum, Origanum, Salvia, and Thymus genera. These plants are a rich source of bioactive molecules such as terpenes, flavonoids, and phenolic acids. In this sense, there is a subgroup of flavonoids classified as flavones. Flavones have antioxidant, anti-inflammatory, anti-cancer, and anti-diabetic potential; thus, efficient extraction techniques from their original plant matrixes have been developed. Currently, conventional extraction methods involving organic solvents are no longer recommended due to their environmental consequences, and new environmentally friendly techniques have been developed. Moreover, once extracted, the bioactivity of flavones is highly linked to their bioavailability, which is often neglected. This review aims to comprehensively gather recent information (2011–2021) regarding extraction techniques and their important relationship with the bioavailability of flavones from Lamiaceae plants including Salvia, Ocimum, Thymus, and Origanum.
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Nallusamy S, Mannu J, Ravikumar C, Angamuthu K, Nathan B, Nachimuthu K, Ramasamy G, Muthurajan R, Subbarayalu M, Neelakandan K. Exploring Phytochemicals of Traditional Medicinal Plants Exhibiting Inhibitory Activity Against Main Protease, Spike Glycoprotein, RNA-dependent RNA Polymerase and Non-Structural Proteins of SARS-CoV-2 Through Virtual Screening. Front Pharmacol 2021; 12:667704. [PMID: 34305589 PMCID: PMC8295902 DOI: 10.3389/fphar.2021.667704] [Citation(s) in RCA: 34] [Impact Index Per Article: 8.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/14/2021] [Accepted: 06/24/2021] [Indexed: 12/17/2022] Open
Abstract
Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) being a causative agent for global pandemic disease nCOVID’19, has acquired much scientific attention for the development of effective vaccines and drugs. Several attempts have been made to explore repurposing existing drugs known for their anti-viral activities, and test the traditional herbal medicines known for their health benefiting and immune-boosting activity against SARS-CoV-2. In this study, efforts were made to examine the potential of 605 phytochemicals from 37 plant species (of which 14 plants were endemic to India) and 139 antiviral molecules (Pubchem and Drug bank) in inhibiting SARS-CoV-2 multiple protein targets through a virtual screening approach. Results of our experiments revealed that SARS-CoV-2 MPro shared significant disimilarities against SARS-CoV MPro and MERS-CoV MPro indicating the need for discovering novel drugs. This study has screened the phytochemical cyanin (Zingiber officinale) which may exhibit broad-spectrum inhibitory activity against main proteases of SARS-CoV-2, SARS-CoV and MERS-CoV with binding energies of (−) 8.3 kcal/mol (−) 8.2 kcal/mol and (−) 7.7 kcal/mol respectively. Amentoflavone, agathisflavone, catechin-7-o-gallate and chlorogenin were shown to exhibit multi-target inhibitory activity. Further, Mangifera indica, Anacardium occidentale, Vitex negundo, Solanum nigrum, Pedalium murex, Terminalia chebula, Azadirachta indica, Cissus quadrangularis, Clerodendrum serratum and Ocimum basilicumaree reported as potential sources of phytochemicals for combating nCOVID’19. More interestingly, this study has highlighted the anti-viral properties of the traditional herbal formulation “Kabasura kudineer” recommended by AYUSH, a unit of Government of India. Short listed phytochemicals could be used as leads for future drug design and development. Genomic analysis of identified herbal plants will help in unraveling molecular complexity of therapeutic and anti-viral properties which proffer lot of chance in the pharmaceutical field for researchers to scout new drugs in drug discovery.
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Affiliation(s)
- Saranya Nallusamy
- Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore, India
| | - Jayakanthan Mannu
- Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore, India
| | - Caroline Ravikumar
- Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore, India
| | - Kandavelmani Angamuthu
- Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore, India
| | - Bharathi Nathan
- Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore, India
| | - Kumaravadivel Nachimuthu
- Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore, India
| | - Gnanam Ramasamy
- Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore, India
| | - Raveendran Muthurajan
- Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India
| | - Mohankumar Subbarayalu
- Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India
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Maccioni A, Falconieri D, Sanna C, Porcedda S, Piras A, Maxia A. Characterization of Essential Oils from Different Taxa Belonging to the Genus Teucrium in Sardinia Island, Italy. PLANTS 2021; 10:plants10071359. [PMID: 34371562 PMCID: PMC8309330 DOI: 10.3390/plants10071359] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 06/03/2021] [Revised: 06/29/2021] [Accepted: 07/01/2021] [Indexed: 12/13/2022]
Abstract
The genus Teucrium L. (Lamiaceae) is a genus growing in mild climate zones, particularly in the Mediterranean Basin and Central Asia. It is represented by 11 taxa in Sardinia (Italy), living commonly in sunny habitats. In this study, the following eight Sardinian Teucrium taxa were selected, and the essential oils (EOs), obtained by stem distillation, were analyzed by GC–FID and GC–MS: T. capitatum subsp. capitatum, T. chamaedrys subsp. chamaedrys, T. flavum subsp. glaucum, T. marum, T. massiliense, T. scordium subsp. scordioides, T. scorodonia, and T. subspinosum. The comprehensive analyses led to the identification of 87 constituents representing the majority of the volatile compounds. Significant differences, both qualitative and quantitative, were observed between the taxa. Overall, monoterpenes and sesquiterpenes characterized all Teucrium EOs: T. capitatum subsp. capitatum and T. flavum subsp. glaucum revealed the highest content of monoterpene hydrocarbons, while in the other Teucrium taxa sesquiterpene hydrocarbons prevailed. Worthy of note, diterpenes were found only in T. marum and T. subspinosum, whereas T. massiliense was rich in non-terpenic oxygenated compounds. To the best of our knowledge, this is the first comprehensive report on the chemical composition of EOs obtained from Sardinian Teucrium species.
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Affiliation(s)
- Alfredo Maccioni
- Co.S.Me.Se, Consorzio per lo Studio dei Metaboliti Secondari, Via Sant’Ignazio da Laconi 13, 09123 Cagliari, Italy; (A.M.); (A.M.)
| | - Danilo Falconieri
- State Industrial Technical Institute “Michele Giua”, Via Montecassino 41, 09134 Cagliari, Italy;
| | - Cinzia Sanna
- Co.S.Me.Se, Consorzio per lo Studio dei Metaboliti Secondari, Via Sant’Ignazio da Laconi 13, 09123 Cagliari, Italy; (A.M.); (A.M.)
- Department of Life and Environmental Sciences, University of Cagliari, Via S. Ignazio da Laconi 13, 09123 Cagliari, Italy
- Correspondence:
| | - Silvia Porcedda
- Department of Chemical and Geological Sciences, University of Cagliari, Cittadella Universitaria, S.S. 554, 09042 Monserrato, Italy; (S.P.); (A.P.)
| | - Alessandra Piras
- Department of Chemical and Geological Sciences, University of Cagliari, Cittadella Universitaria, S.S. 554, 09042 Monserrato, Italy; (S.P.); (A.P.)
| | - Andrea Maxia
- Co.S.Me.Se, Consorzio per lo Studio dei Metaboliti Secondari, Via Sant’Ignazio da Laconi 13, 09123 Cagliari, Italy; (A.M.); (A.M.)
- Department of Life and Environmental Sciences, University of Cagliari, Via S. Ignazio da Laconi 13, 09123 Cagliari, Italy
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Evangelina IA, Herdiyati Y, Laviana A, Rikmasari R, Zubaedah C, Anisah, Kurnia D. Bio-Mechanism Inhibitory Prediction of β-Sitosterol from Kemangi ( Ocimum basilicum L.) as an Inhibitor of MurA Enzyme of Oral Bacteria: In vitro and in silico Study. Adv Appl Bioinform Chem 2021; 14:103-115. [PMID: 34188494 PMCID: PMC8236250 DOI: 10.2147/aabc.s301488] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/01/2021] [Accepted: 04/20/2021] [Indexed: 11/23/2022] Open
Abstract
Background Dental caries is a widespread disease that causes dental tissue destruction and leads to local and general complications. Gram-positive bacteria including Streptococcus mutans, Streptococcus sanguinis, and Enterococcus faecalis take part in dental caries formation. Gram-positive bacteria have cell walls that consistof a thick layer of peptidoglycan which maintains the strength and rigidity of the bacteria, as well as bacteria guard from internal osmotic pressure. The biosynthesis of peptidoglycan involves many enzymes, including the Mur family, penicillin binding protein (PBP), and sortases. Purpose This research has the intention to screen and examine the antibacterial compound of edible plant Kemangi (Ocimum basilicum L.) in terms of how it fights against some oral pathogenic bacteria of E. faecalis ATCC 29212, S. mutans ATCC 25175, and S. sanguinis ATCC 10566. Materials and Methods The O. basilicum L. was macerated by several organic solvents to obtain the extracts, before then being purified using several combinations of chromatography methods and the compound was discovered via spectroscopic methods. For the assay against bacteria, the extracts and compounds were tested using agar well diffusion and microdilution assay. Results The isolated compound was identified as β-sitosterol. The compound activity against bacteria was evaluated by in vitro assay against S. sanguinis ATCC 10566 and E. faecalis ATCC 29212 with the MIC and MBC value of 25,000 and 50,000 ppm, respectively. The compound was also tested by in silico study using the molecular docking method. The molecular interaction between β-sitosterol and the protein target showed a lower binding affinity value than the native ligand and other positive controls for each protein. Based on the amino acid residue bound to the ligands, β-sitosterol on MurA and SrtA is not competitive to the positive control, showing potential as a natural antibacterial agent. Meanwhile, on the MurB and PBP, β-sitosterol and positive control do compete with each other. Conclusion The compound, isolated from O. basilicum L. leaf, was determined as β-sitosterol, which has the molecular formula C29H50O. The antibacterial activity of β-sitosterol by in vitro assay showed weak antibacterial activity, yet exhibited the potential to inhibit the biosynthesis of peptidoglycan and prevent bacteria cell wall formation by inhibiting MurA and SrtA activity via docking simulation.
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Affiliation(s)
- Ida Ayu Evangelina
- Department of Orthodontics, Faculty of Dentistry - Universitas Padjadjaran, Bandung, Indonesia
| | - Yetty Herdiyati
- Department of Pediatric Dentistry, Faculty of Dentistry - Universitas Padjadjaran, Bandung, Indonesia
| | - Avi Laviana
- Department of Orthodontics, Faculty of Dentistry - Universitas Padjadjaran, Bandung, Indonesia
| | - Rasmi Rikmasari
- Department of Prosthodontics, Faculty of Dentistry - Universitas Padjadjaran, Bandung, Indonesia
| | - Cucu Zubaedah
- Department of Dental Public Health, Faculty of Dentistry - Universitas Padjadjaran, Bandung, Indonesia
| | - Anisah
- Department of Chemistry, Faculty of Mathematics and Natural Science - Universitas Padjadjaran, Sumedang, Indonesia
| | - Dikdik Kurnia
- Department of Chemistry, Faculty of Mathematics and Natural Science - Universitas Padjadjaran, Sumedang, Indonesia
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Antibacterial Potency of Medicinal Plants including Artemisia annua and Oxalis corniculata against Multi-Drug Resistance E. coil. BIOMED RESEARCH INTERNATIONAL 2021; 2021:9981915. [PMID: 34124267 PMCID: PMC8189797 DOI: 10.1155/2021/9981915] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/20/2021] [Revised: 05/11/2021] [Accepted: 05/13/2021] [Indexed: 12/16/2022]
Abstract
Antibacterial activity of ethanolic and aqueous extracts of two medicinal plants including Oxalis corniculata (EtOc, AqOc) and Artemisia annua (EtAa, AqAa) as well as A. annua essential oil (EoAa) was investigated on multi-drug resistance (MDR) E. coli. Microdilution and agar well diffusion methods were used to determine the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) as well as the inhibition zone. The phytconstituents of these products were analyzed using Reverse-phase High- performance liquid chromatography (RP-HPLC) and gas chromatography-mass spectrometry (GC-mass). The order of bacteriostatic and bacteriocide rate of the products can be shown as follows: EoAa>AqOc>EtAa = AqAa>EtOc, but the bactericidal effect of A. annua extracts is higher than of O. corniculata based on the MIC/MBC ratio and the order is as follows: EoAa>EtAa = AqAa>EtOc>AqOc. The most potent product, i.e. EoAa with a 56.7% inhibition of all isolates, has the potential to substitute 13 used antibiotics including oxacillin, amoxicillin, ampicillin, amoxicillin-clavulanic acid, tetracycline, streptomycin, ciprofloxacin, ceftriaxone, cefazolin, cefuroxime, cefotaxime, ceftazidime and cefixime (P <0.05). Different terpenoids were detected and measured in EoAa and catechin flavonoids in extracts of both plants, quercetin in extracts of O. corniculata but it was only possible to detect chlorogenic acid polyphenol in AqAa. Due to the antibacterial activities of the studied products, more effective than some antibiotics and their edible consumption, these products can be suggested as an alternative to some antibiotics and food preservatives to fight against MDR E. coli.
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Comparative analysis on bioactive compounds and antioxidant activity of Algerian fenugreek (Trigonella foenum-graecum L.) and Syrian cumin (Cuminum cyminum L.) seeds. HERBA POLONICA 2021. [DOI: 10.2478/hepo-2021-0005] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/20/2022]
Abstract
Summary
Introduction: Natural products represent a gold mine for scientists looking for compounds for the treatment of health problems and diseases with their different biological and pharmacological activities. However, recent research is focused on finding natural sources of antioxidants.
Objective: The objective of current research was to determine the phytochemical profile of Algerian fenu-greek (Trigonella foenum-graecum L.), and Syrian cumin (Cuminum cyminum L.) seeds in order to characterize their phenolic compounds and to determine their antioxidant activities.
Methods: Total phenolic, flavonoids, condensed and hydrolysable tannins contents were quantified using Folin-Ciocalteu, aluminium chloride, vanillin and ferric chloride methods, respectively. Phenolic compounds were identified by HPLC method and antioxidant activity was measured using DPPH assay.
Results: The higher amounts of total phenolic compounds, flavonoids, condensed and hydrolysable tannins were given by fenugreek. Results of HPLC analysis of our plants showed that eight phytochemical compounds were found in cumin extract, and seven molecules in fenugreek extract. Moreover, fenugreek possessed higher antioxidant activity.
Conclusion: This study confirmed that our plants are a good source of phenolic contents and possess a high antioxidant activity.
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Babich O, Sukhikh S, Pungin A, Astahova L, Chupakhin E, Belova D, Prosekov A, Ivanova S. Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds. PLANTS (BASEL, SWITZERLAND) 2021; 10:plants10050915. [PMID: 34063290 PMCID: PMC8147393 DOI: 10.3390/plants10050915] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/20/2021] [Revised: 04/28/2021] [Accepted: 04/29/2021] [Indexed: 05/08/2023]
Abstract
The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of Hyssopus officinalis were chosen for study. Nutrient media of various compositions were used for Hyssopus officinalis callus culture. For each culture, data on the quantitative contents of saponins, flavonoids and polyphenolic compounds, as well as antioxidant activity, were obtained. It was found that Murashige and Skoog medium supplemented with 1-naphthylacetic acid and kinetin led to the highest yield of secondary metabolites.
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Affiliation(s)
- Olga Babich
- Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia; (O.B.); (S.S.); (A.P.); (L.A.); (E.C.); (D.B.)
- Laboratory of Biocatalysis, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia;
| | - Stanislav Sukhikh
- Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia; (O.B.); (S.S.); (A.P.); (L.A.); (E.C.); (D.B.)
- Laboratory of Biocatalysis, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia;
| | - Artem Pungin
- Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia; (O.B.); (S.S.); (A.P.); (L.A.); (E.C.); (D.B.)
| | - Lidiya Astahova
- Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia; (O.B.); (S.S.); (A.P.); (L.A.); (E.C.); (D.B.)
| | - Evgeny Chupakhin
- Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia; (O.B.); (S.S.); (A.P.); (L.A.); (E.C.); (D.B.)
| | - Daria Belova
- Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia; (O.B.); (S.S.); (A.P.); (L.A.); (E.C.); (D.B.)
| | - Alexander Prosekov
- Laboratory of Biocatalysis, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia;
| | - Svetlana Ivanova
- Natural Nutraceutical Biotesting Laboratory, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia
- Department of General Mathematics and Informatics, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia
- Correspondence: ; Tel.: +7-384-239-6832
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Shanaida M, Hudz N, Jasicka-Misiak I, Wieczorek PP. Polyphenols and Pharmacological Screening of a Monarda fistulosa L. dry Extract Based on a Hydrodistilled Residue By-Product. Front Pharmacol 2021; 12:563436. [PMID: 33995001 PMCID: PMC8118672 DOI: 10.3389/fphar.2021.563436] [Citation(s) in RCA: 17] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/18/2020] [Accepted: 02/15/2021] [Indexed: 02/02/2023] Open
Abstract
This study aimed to determine the composition and content of polyphenols in the dry extract obtained from the hydrodistilled residue by-product of the wild bergamot (Monarda fistulosa L., Lamiaceae Martinov family) herb (MFDE) and to evaluate its safety and pharmacological properties. The total phenolic content (TPC) in the MFDE was 120.64 mg GAE/g. The high-performance liquid chromatography (HPLC) analysis showed the presence of a plethora of phenolic compounds, including hydroxycinnamic acids and flavone derivatives in the MFDE, with rosmarinic acid and luteolin-7-O-glucoside being the main components. With an IC50 value of 0.285 mg/mL, it was found to be a strong DPPH radical scavenger. The acute toxicity study results indicate that the oral administration of MFDE to rats at the doses of 500–5,000 mg/kg did not produce any side effects or death in animals which indicates its safety. The results of the in vivo assay showed that the MFDE dose-dependently inhibited paw oedema and significantly reduced the number of writings in mice induced by the acetic acid injection suggesting its potent anti-inflammatory and analgesic activities, respectively. The conducted studies revealed that M. fistulosa hydrodistilled residue by-product could be regarded as a new natural source of polyphenols with valuable pharmacological properties.
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Affiliation(s)
- Mariia Shanaida
- Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
| | - Nataliia Hudz
- Department of Drug Technology and Biopharmaceutics, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
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de Munter J, Pavlov D, Gorlova A, Sicker M, Proshin A, Kalueff AV, Svistunov A, Kiselev D, Nedorubov A, Morozov S, Umriukhin A, Lesch KP, Strekalova T, Schroeter CA. Increased Oxidative Stress in the Prefrontal Cortex as a Shared Feature of Depressive- and PTSD-Like Syndromes: Effects of a Standardized Herbal Antioxidant. Front Nutr 2021; 8:661455. [PMID: 33937310 PMCID: PMC8086427 DOI: 10.3389/fnut.2021.661455] [Citation(s) in RCA: 23] [Impact Index Per Article: 5.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/30/2021] [Accepted: 03/02/2021] [Indexed: 12/30/2022] Open
Abstract
Major depression (MD) and posttraumatic stress disorder (PTSD) share common brain mechanisms and treatment strategies. Nowadays, the dramatically developing COVID-19 situation unavoidably results in stress, psychological trauma, and high incidence of MD and PTSD. Hence, the importance of the development of new treatments for these disorders cannot be overstated. Herbal medicine appears to be an effective and safe treatment with fewer side effects than classic pharmaca and that is affordable in low-income countries. Currently, oxidative stress and neuroinflammation attract increasing attention as important mechanisms of MD and PTSD. We investigated the effects of a standardized herbal cocktail (SHC), an extract of clove, bell pepper, basil, pomegranate, nettle, and other plants, that was designed as an antioxidant treatment in mouse models of MD and PTSD. In the MD model of “emotional” ultrasound stress (US), mice were subjected to ultrasound frequencies of 16–20 kHz, mimicking rodent sounds of anxiety/despair and “neutral” frequencies of 25–45 kHz, for three weeks and concomitantly treated with SHC. US-exposed mice showed elevated concentrations of oxidative stress markers malondialdehyde and protein carbonyl, increased gene and protein expression of pro-inflammatory cytokines interleukin (IL)-1β and IL-6 and other molecular changes in the prefrontal cortex as well as weight loss, helplessness, anxiety-like behavior, and neophobia that were ameliorated by the SHC treatment. In the PTSD model of the modified forced swim test (modFST), in which a 2-day swim is followed by an additional swim on day 5, mice were pretreated with SHC for 16 days. Increases in the floating behavior and oxidative stress markers malondialdehyde and protein carbonyl in the prefrontal cortex of modFST-mice were prevented by the administration of SHC. Chromatography mass spectrometry revealed bioactive constituents of SHC, including D-ribofuranose, beta-D-lactose, malic, glyceric, and citric acids that can modulate oxidative stress, immunity, and gut and microbiome functions and, thus, are likely to be active antistress elements underlying the beneficial effects of SHC. Significant correlations of malondialdehyde concentration in the prefrontal cortex with altered measures of behavioral despair and anxiety-like behavior suggest that the accumulation of oxidative stress markers are a common biological feature of MD and PTSD that can be equally effectively targeted therapeutically with antioxidant therapy, such as the SHC investigated here.
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Affiliation(s)
- Johannes de Munter
- Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University, Maastricht, Netherlands
| | - Dmitrii Pavlov
- Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University, Maastricht, Netherlands.,Laboratory of Psychiatric Neurobiology, Institute of Molecular Medicine and Department of Normal Physiology, Sechenov First Moscow State Medical University, Moscow, Russia
| | - Anna Gorlova
- Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University, Maastricht, Netherlands.,Laboratory of Psychiatric Neurobiology, Institute of Molecular Medicine and Department of Normal Physiology, Sechenov First Moscow State Medical University, Moscow, Russia
| | - Michael Sicker
- Rehabilitation Research Unit of Clinic of Bad Kreuzbach, Bad Kreuzbach, Germany
| | - Andrey Proshin
- PK Anokhin Research Institute of Normal Physiology, Moscow, Russia
| | - Allan V Kalueff
- Ural Federal University, Yekaterinburg, Russia.,Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia.,Neuroscience Program, Sirius University, Sochi, Russia.,School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Moscow, Russia
| | - Andrey Svistunov
- Institute for Translational Medicine and Biotechnology, Preclinical Research Center of Sechenov First Moscow State Medical University, Moscow, Russia
| | - Daniel Kiselev
- Laboratory of Psychiatric Neurobiology, Institute of Molecular Medicine and Department of Normal Physiology, Sechenov First Moscow State Medical University, Moscow, Russia.,Institute for Translational Medicine and Biotechnology, Preclinical Research Center of Sechenov First Moscow State Medical University, Moscow, Russia.,Federal Budgetary Institute of General Pathology and Pathophysiology, Moscow, Russia
| | - Andrey Nedorubov
- Institute for Translational Medicine and Biotechnology, Preclinical Research Center of Sechenov First Moscow State Medical University, Moscow, Russia
| | - Sergey Morozov
- Federal Budgetary Institute of General Pathology and Pathophysiology, Moscow, Russia
| | - Aleksei Umriukhin
- Laboratory of Psychiatric Neurobiology, Institute of Molecular Medicine and Department of Normal Physiology, Sechenov First Moscow State Medical University, Moscow, Russia
| | - Klaus-Peter Lesch
- Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University, Maastricht, Netherlands.,Laboratory of Psychiatric Neurobiology, Institute of Molecular Medicine and Department of Normal Physiology, Sechenov First Moscow State Medical University, Moscow, Russia.,Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany
| | - Tatyana Strekalova
- Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University, Maastricht, Netherlands.,Laboratory of Psychiatric Neurobiology, Institute of Molecular Medicine and Department of Normal Physiology, Sechenov First Moscow State Medical University, Moscow, Russia.,Federal Budgetary Institute of General Pathology and Pathophysiology, Moscow, Russia.,Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany
| | - Careen A Schroeter
- Department of Preventive Medicine, Maastricht Medical Center Annadal, Maastricht, Netherlands
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Mićović T, Topalović D, Živković L, Spremo-Potparević B, Jakovljević V, Matić S, Popović S, Baskić D, Stešević D, Samardžić S, Stojanović D, Maksimović Z. Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of Hyssopus officinalis L. Subsp. aristatus (Godr.) Nyman (Lamiaceae). PLANTS 2021; 10:plants10040711. [PMID: 33916934 PMCID: PMC8067569 DOI: 10.3390/plants10040711] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 02/25/2021] [Revised: 03/29/2021] [Accepted: 03/30/2021] [Indexed: 11/16/2022]
Abstract
Hyssopus officinalis L. is a well-known aromatic plant used in traditional medicine and the food and cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic and cytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical composition was analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diode array detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxic and antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480, MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oils were rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the most abundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively). Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC50 = 56.04–199.89 µg/mL, FRAP = 0.667–0.959 mmol Fe2+/g). Hyssop preparations significantly reduced DNA damage in human whole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selective and potent dose- and time-dependent activity against the HeLa cell line. Results of the current study demonstrated notable H. officinalis medicinal potential, which calls for further investigation.
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Affiliation(s)
- Tijana Mićović
- Institute for Medicines and Medical Devices of Montenegro, Bulevar Ivana Crnojevića 64a, 81000 Podgorica, Montenegro;
| | - Dijana Topalović
- Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia; (D.T.); (L.Ž.); (B.S.-P.)
| | - Lada Živković
- Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia; (D.T.); (L.Ž.); (B.S.-P.)
| | - Biljana Spremo-Potparević
- Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia; (D.T.); (L.Ž.); (B.S.-P.)
| | - Vladimir Jakovljević
- Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, Serbia;
- Department of Human Pathology, First Moscow State Medical University I. M. Sechenov, Trubetskaya Street 8, Str. 2, 119991 Moscow, Russia
| | - Sanja Matić
- Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, Serbia;
| | - Suzana Popović
- Department of Microbiology and Immunology, Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, Serbia; (S.P.); (D.B.)
| | - Dejan Baskić
- Department of Microbiology and Immunology, Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, Serbia; (S.P.); (D.B.)
- Public Health Institute, Nikole Pašića 1, 34000 Kragujevac, Serbia
| | - Danijela Stešević
- Faculty of Natural Sciences and Mathematics, University of Montenegro, Džordža Vašingtona bb, 81000 Podgorica, Montenegro;
| | - Stevan Samardžić
- Department of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia;
| | - Danilo Stojanović
- Department of Botany, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia;
| | - Zoran Maksimović
- Department of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia;
- Correspondence:
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Oalđe M, Kolarević S, Živković J, Alimpić Aradski A, Jovanović Marić J, Kračun Kolarević M, Đorđević J, Marin PD, Šavikin K, Vuković-Gačić B, Duletić-Laušević S. A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. Food Funct 2021; 12:3233-3245. [PMID: 33877247 DOI: 10.1039/d1fo00447f] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
Abstract
This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g-1), naringin (up to 5.07 mg g-1) and luteolin-7-O-glucoside (up to 3.54 mg g-1). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.
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Affiliation(s)
- Mariana Oalđe
- Institute of Botany and Botanical Garden "Jevremovac", Chair of Plant Morphology and Systematics, Faculty of Biology, University of Belgrade, Belgrade, Serbia.
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Reneva M, Gubanov V, Gubanova V. Yield and content of essential oils in the common origanum nursery. BIO WEB OF CONFERENCES 2021. [DOI: 10.1051/bioconf/20213601002] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/14/2022] Open
Abstract
Common origanum is one of the most famous spices, it is popular all over the world. The purpose of this work is to conduct a comparative assessment of the yield of raw plant materials and the content of essential oils in common origanum plants. Field research was carried out in 2019-2020 at the experimental field of the Northern Trans Urals Research Institute - a branch of the Tyumen Research Center SB RAS (Moskovsky settlement of the Tyumen district). The soil of the experimental site belongs to the subtype of dark gray forest, heavy loamy according to the granulometric composition. From the studied 350 common origanum samples, 16 samples were isolated. All samples were evaluated according to the following criteria: the yield of raw plant materials and the amount of essential oil. The Khutoryanka variety is accepted as the standard. For two years of research, three samples were distinguished from the studied samples of common origanum in terms of the yield of raw plant materials compared to the standard: 12-2-2; 13-2-1; 15-2-1, the yield of these numbers was from 3.6 to 3.9 t/ha, this is (13.3, 10.0, 6.6 %) higher than the standard. From the studied samples of common origanum, two samples were distinguished by the content of essential oils in dry plant materials - 14-2-3 and 21-1-1. The harvest per hectare of these samples was, respectively, 750 and 928 ml in 2019, and in 2020, 1026 and 1120 ml/ha.
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KASKA A, DENIZ N, ÇIÇEK M, MAMMADOV R. The screening of Digitalis ferruginea L. subsp. ferruginea for toxic capacities, phenolic constituents, antioxidant properties, mineral elements and proximate analysis. FOOD SCIENCE AND TECHNOLOGY 2021. [DOI: 10.1590/fst.08620] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 02/08/2023]
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Özel Ş, Süntar İ, Ercan Gökay N, Taşkın Türkmenoğlu T, Demırel MA. The effectiveness of Teucrium chamaedrys L. extracts on endometriotic implant regression in rat endometriosis model. VETERINARY RESEARCH FORUM : AN INTERNATIONAL QUARTERLY JOURNAL 2020; 11:305-309. [PMID: 33643581 PMCID: PMC7904131 DOI: 10.30466/vrf.2019.105229.2500] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/15/2019] [Accepted: 07/06/2019] [Indexed: 01/23/2023]
Abstract
The aim of the present study was to investigate the therapeutic effects of Teucrium chamaedrys L. (Lamiaceae) in the experimentally induced endometriosis in rats. Endometrial tissue was implanted into the abdominal wall of thirty Sprague Dawley rats; the rats with endometriosis were randomized into five groups and treatment procedure was performed for three weeks. The treatment groups were orally treated with three different extracts of Teucrium chamaedrys. Buserelin acetate (20.00 mg) was given as a reference drug. Vehicle was administered alone to the control group. All rats were sacrified at the end of the experiment. The endometriotic implants were measured, intra-abdominal adhesions were scored and the tissue samples were histopathologically investigated. After the treatment procedure, the volumes of endometrial implant and adhesions were detected to be significantly decreased in the T. chamaedrys extracts treated groups compared to the control group. Therapeutic effect of the T. chamaedrys extracts could be attributed to the both nonpolar and polar secondary metabolites. The study conceived that the different polarity extracts of T. chamaedrys could be beneficial in the treatment of endometriosis.
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Affiliation(s)
- Şule Özel
- Clinic of Gynecology, Zekai Tahir Burak Woman's Health Training and Research Hospital, University of Health Sciences, Ankara, Turkey
| | - İpek Süntar
- Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, Turkey
| | - Nilüfer Ercan Gökay
- Clinic of Gynecology, Zekai Tahir Burak Woman's Health Training and Research Hospital, University of Health Sciences, Ankara, Turkey
| | - Tuğba Taşkın Türkmenoğlu
- Department of Pathology, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey
| | - Mürşide Ayşe Demırel
- Laboratory Animals Care and Research Unit, Department of Pharmacology, Faculty of Pharmacy, Gazi University, Ankara, Turkey
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Faur A, Watz C, Moacă EA, Avram Ş, Borcan F, Pinzaru I, Iftode A, Nicolov M, Popovici RA, Raica M, Szuhanek CA, Dehelean C. Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient. Molecules 2020; 25:molecules25225442. [PMID: 33233640 PMCID: PMC7699777 DOI: 10.3390/molecules25225442] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/29/2020] [Revised: 11/17/2020] [Accepted: 11/17/2020] [Indexed: 11/26/2022] Open
Abstract
The current study was aimed to evaluate the phenolic composition parameters of two hydro-alcoholic extracts of Ocimum basilicum L. (OB) obtained from the aerial part (without leaves) and leaves, in order to determine their contribution to the antioxidant activity (AOA). Both hydro-alcoholic extracts have proven to be rich in polyphenolic compounds, flavonoids, flavonols and tannins. Therefore, the leaves’ extracts reveal an inhibition percentage of 89%, almost comparable with the standard reference (95%). To complete the toxicological profile, the study also assessed the potential cytotoxicity of basil hydro-alcoholic extracts on immortalized human keratinocytes (HaCaT), skin human fibroblasts (1BR3), mice epidermis (JB6Cl41-5a) and primary human melanocytes (HEMa) cells, correlated to A375 antitumor in vitro activity. The extracts did not induce significant cytotoxic effect on any of the selected normal cell lines but showed relevant activity on A375 cells. Considering the low values obtained regarding the irritative effects in the chorionallantoic membrane of the egg on blood vessels, we can emphasize that both extracts can be considered as biocompatible ingredients. Regarding the potential activity of hydro-alcoholic extracts on human skin, the decrease of erythema values after the application of extracts was a relevant observation which indicates the anti-inflammatory potential of Ocimum basilicum L.
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Affiliation(s)
- Alin Faur
- Department of Microscopic Morphology/Histology, Angiogenesis Research Center, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania; (A.F.); (M.R.)
| | - Claudia Watz
- Department of Pharmaceutical Physics, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania; (C.W.); (M.N.)
| | - Elena-Alina Moacă
- Department of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania; (I.P.); (A.I.); (C.D.)
- Correspondence: ; Tel.: +40-745-762-600
| | - Ştefana Avram
- Department of Pharmacognosy, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania;
| | - Florin Borcan
- Department of Analytical Chemistry, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania;
| | - Iulia Pinzaru
- Department of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania; (I.P.); (A.I.); (C.D.)
| | - Andrada Iftode
- Department of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania; (I.P.); (A.I.); (C.D.)
| | - Mirela Nicolov
- Department of Pharmaceutical Physics, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania; (C.W.); (M.N.)
| | - Ramona Amina Popovici
- Department of Management, Legislation and Communication in Dentistry, Faculty of Dentistry, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania;
| | - Marius Raica
- Department of Microscopic Morphology/Histology, Angiogenesis Research Center, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania; (A.F.); (M.R.)
| | - Camelia A. Szuhanek
- Department of Orthodontics, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania;
| | - Cristina Dehelean
- Department of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania; (I.P.); (A.I.); (C.D.)
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Babich O, Sukhikh S, Prosekov A, Asyakina L, Ivanova S. Medicinal Plants to Strengthen Immunity during a Pandemic. Pharmaceuticals (Basel) 2020; 13:E313. [PMID: 33076514 PMCID: PMC7602650 DOI: 10.3390/ph13100313] [Citation(s) in RCA: 28] [Impact Index Per Article: 5.6] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/18/2020] [Revised: 10/11/2020] [Accepted: 10/13/2020] [Indexed: 02/07/2023] Open
Abstract
The development of new effective anti-coronavirus drugs and therapies is important, but it requires significant human, financial and, most importantly, time expenditures. The current pandemic is neither the first nor the last. Humanity has already accumulated considerable survival experience. We cannot do without prevention and epidemiological protection measures. This study reviews medicinal plants that grow in Northeast Asia and whose antioxidant, antiviral, anti-inflammatory and immunomodulatory characteristics are already known, also in the framework of the prevention and treatment of pneumonia of various etiologies. The need for a comprehensive approach to maintaining immunodefences, including functional foods and positive emotions, is emphasized. In the period of pandemics, it is important to research various areas that allow to us accumulate a critical mass of information and cope with the next global disease.
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Affiliation(s)
- Olga Babich
- Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia; (O.B.); (S.S.)
- Laboratory of Biocatalysis, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia;
| | - Stanislav Sukhikh
- Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia; (O.B.); (S.S.)
- Department of Bionanotechnology, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia;
| | - Alexander Prosekov
- Laboratory of Biocatalysis, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia;
| | - Lyudmila Asyakina
- Department of Bionanotechnology, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia;
| | - Svetlana Ivanova
- Natural Nutraceutical Biotesting Laboratory, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia
- Department of General Mathematics and Informatics, Kemerovo State University, Krasnaya Street, 6, 650043 Kemerovo, Russia
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Dumas NGE, Anderson NTY, Godswill NN, Thiruvengadam M, Ana-Maria G, Ramona P, Crisan GC, Laurian V, Shariati MA, Tokhtarov Z, Emmanuel Y. Secondary metabolite contents and antimicrobial activity of leaf extracts reveal genetic variability of Vernonia amygdalina and Vernonia calvoana morphotypes. Biotechnol Appl Biochem 2020; 68:938-947. [PMID: 32881085 DOI: 10.1002/bab.2017] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/04/2020] [Accepted: 08/12/2020] [Indexed: 01/14/2023]
Abstract
Vernonia species (Asteraceae) are indigenous medicinal and food leaf vegetables commonly consumed in some African countries like Cameroon where they constitute a staple dish called "ndolé." Previous studies have demonstrated the nutritional importance of Vernonia, but there is little knowledge of their agronomic value and genetic potentials. Wide genetic variability in the genus has been established earlier through the study of its pollen. However, to the best of our understanding, no such study has been undertaken on the genetic variability of Vernonia with respect to its secondary metabolites. This study was therefore aimed at evaluating the genetic variability of Vernonia amygdalina (VAA and VALF) and V. calvoana (VCAB, VCAV, VCSB, and VCSV) morphotypes in 2016 based on secondary metabolite content and antimicrobial properties. This involved phytochemical analysis by HPLC/MS for the detection of alkaloids, flavonoids, phenols, and tannins in leaves from each of the six genotypes. Results showed that all tested genotypes are rich in phenols (18 analyzed), flavonoids, and tannins with, VAA richer in phenols (206.1 ± 3.12 µg/g of dry extract), followed by VCAV (197.9 ± 18.03 µg/g). The lowest level of flavonoid was found in VCSV (81.6 ± 7.21 µg/g), while the highest was from VCAB (132.8 ± 31.5 µg/g). VCSV (56.3 ± 4.08 µg/g) had the lowest level of tannins, while VCAB (97.8 ± 23.8 µg/g) had the highest levels. Assessment of antimicrobial activity of leaf extracts from the six genotypes was done by culture on Mueller Hinton (MH) agar and MH broth agar for bacteria and in Sabouraud dextrose agar and Sabouraud dextrose broth media for fungi, respectively. All morphotypes exhibited inhibitory activity on bacteria except VAA, with isoquercetin characteristic of VCSV. Chemotaxonomic analyses of Vernonia morphotypes highlighted the genetic diversity within species and variability of antimicrobial properties of ethanolic leaf extracts among morphotypes. These results provide baseline data in the valuation of genetic resources and the establishment of improvement programs.
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Affiliation(s)
| | - Ngandjui Tchangoue Yvan Anderson
- Department of Organic Chemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon.,Laboratory of Pharmaceutical Botany, Faculty of Pharmacy, University of Medicine and Pharmacy "Luliu Hatieganu" Str. Gheorghe Marinescu, Cluj-Napoca, Cluj, 400337, Romania
| | - Ntsomboh-Ntsefong Godswill
- Faculty of Science, Department of Plant Biology, University of Yaounde I, Yaounde, Cameroon.,Directorate of Research Valorisation and Innovation, Institute of Agricultural Research for Development (IRAD), Yaounde, Cameroon
| | - Muthu Thiruvengadam
- Department of Crop Science, College of Sanghuh Life Science, Konkuk University, Seoul, 05029, Republic of Korea
| | - Gheldiu Ana-Maria
- Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy "Luliu Hatieganu" 8, Victor Babes, Cluj-Napoca, Cluj, 400012, Romania
| | - Paltinean Ramona
- Laboratory of Pharmaceutical Botany, Faculty of Pharmacy, University of Medicine and Pharmacy "Luliu Hatieganu" Str. Gheorghe Marinescu, Cluj-Napoca, Cluj, 400337, Romania
| | - Gianina Cristina Crisan
- Laboratory of Pharmaceutical Botany, Faculty of Pharmacy, University of Medicine and Pharmacy "Luliu Hatieganu" Str. Gheorghe Marinescu, Cluj-Napoca, Cluj, 400337, Romania
| | - Vlase Laurian
- Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy "Luliu Hatieganu" 8, Victor Babes, Cluj-Napoca, Cluj, 400012, Romania
| | - Mohammad Ali Shariati
- Department of Technology of Food Production, K. G. Razumovsky Moscow State University of Technologies and Management (The First Cossack University), Moscow, 109004, Russian Federation
| | | | - Youmbi Emmanuel
- Faculty of Science, Department of Plant Biology, University of Yaounde I, Yaounde, Cameroon.,Tissue Culture Laboratory, African Centre for Research on Banana and Plantain (CARBAP), Njombe, Cameroon
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Effects of sorbitol on the production of phenolic compounds and terpenoids in the cell suspension cultures of Ocimum basilicum L. Biologia (Bratisl) 2020. [DOI: 10.2478/s11756-020-00581-0] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/01/2023]
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Stoleru V, Inculet SC, Mihalache G, Cojocaru A, Teliban GC, Caruso G. Yield and Nutritional Response of Greenhouse Grown Tomato Cultivars to Sustainable Fertilization and Irrigation Management. PLANTS 2020; 9:plants9081053. [PMID: 32824850 PMCID: PMC7464101 DOI: 10.3390/plants9081053] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 07/03/2020] [Revised: 08/13/2020] [Accepted: 08/17/2020] [Indexed: 01/28/2023]
Abstract
Tomato is considered one of the most important crops worldwide from nutritional and economic standpoints, and, in this respect, sustainable production should be a prime objective, particularly in terms of fertilization and irrigation management. The aim of this study was to compare the effects of two fertilization types (chemical or organic) and two irrigation regimes (67% or 100% of evapotranspiration replenishment) on biometrical, biochemical, and yield parameters of three indeterminate cultivars of tomato grown in a greenhouse. The results showed that the effect of organic fertilization was better compared to chemical fertilization for lycopene accumulation and antioxidant activity, as well as for the lower concentrations of any of the macroelements in the tomato fruits; therefore, organic fertilization can be used as an alternative to chemical fertilization in sustainable horticulture. In each cultivar under the same fertilization type, the effect of irrigation was significant on yield and the number of fruits, but the 100% evapotranspiration restoration did not enhance the fruit concentration of all the macroelements and microelements compared to 67% irrigation regime. Higher concentrations of macro- and microelements in the chemically fertilized fruits compared to the organic ones, regardless of the cultivar and the irrigation regime, suggest that the inorganic substances are more easily absorbed by plants under a protected environment. Organic fertilization positively affected the lycopene and antioxidant activities of tomato fruits, thus proving to be a valuable alternative to chemical fertilization in sustainable agriculture, although the product premium quality also depends on the cultivar used.
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Affiliation(s)
- Vasile Stoleru
- Department of Horticultural Technologies, “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, 3 M. Sadoveanu, 700440 Iasi, Romania; (G.M.); (A.C.); (G.-C.T.)
- Correspondence: (V.S.); (S.-C.I.)
| | - Simona-Carmen Inculet
- Department of Horticultural Technologies, “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, 3 M. Sadoveanu, 700440 Iasi, Romania; (G.M.); (A.C.); (G.-C.T.)
- Correspondence: (V.S.); (S.-C.I.)
| | - Gabriela Mihalache
- Department of Horticultural Technologies, “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, 3 M. Sadoveanu, 700440 Iasi, Romania; (G.M.); (A.C.); (G.-C.T.)
- Integrated Center of Environmental Science Studies in the North East Region (CERNESIM), The “Alexandru Ioan Cuza” University of Iasi, 700449 Iasi, Romania
| | - Alexandru Cojocaru
- Department of Horticultural Technologies, “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, 3 M. Sadoveanu, 700440 Iasi, Romania; (G.M.); (A.C.); (G.-C.T.)
| | - Gabriel-Ciprian Teliban
- Department of Horticultural Technologies, “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, 3 M. Sadoveanu, 700440 Iasi, Romania; (G.M.); (A.C.); (G.-C.T.)
| | - Gianluca Caruso
- Department of Agricultural Sciences, University of Naples Federico II, Naples, 80055 Portici, Italy;
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