Melatonin restores zinc levels, activates the Keap1/Nrf2 pathway, and modulates endoplasmic reticular stress and HSP in rats with chronic hepatotoxicity

Figure 1 Western blot analysis. A: Cytoplasmic and nuclear fractions were analyzed by Western blot with antibodies against Kelchlike-ECH-associated protein 1, nuclear factor (erythroid-derived 2)-like 2, and β-actin; B: Arbitrary values expressed as the mean and standard deviation. ^aP < 0.05 CCl₄vs other groups. MLT: Melatonin.

Figure 2 Western blot analysis. A: Nuclear and cytoplasmic fractions were analyzed by Western blot with antibodies against activating transcription factor 6, glucose-regulated protein 78, and glyceraldehyde 3-phosphate dehydrogenase; B: Arbitrary values expressed as the mean and standard deviation. ^aP < 0.05 CCl₄vs other groups. MLT: Melatonin.

Figure 3 Western blot analysis. A: Nuclear and cytoplasmic fractions were analyzed by Western blot with antibodies against heat shock factor 1, heat-shock protein (HSP70), and β-actin; B: Arbitrary values expressed as the mean and standard deviation. ^aP < 0.05 CCl₄vs other groups. MLT: Melatonin.