Xu JX, Wu Y, Zhang L, Wu YH, Li CL, Lin F. Correlation of APOE, SLCO1B1 and LPA KIV-2 gene polymorphisms with coronary heart disease in the Teochew population. World J Cardiol 2025; 17(9): 110278 [DOI: 10.4330/wjc.v17.i9.110278]
Corresponding Author of This Article
Fen Lin, Chief, Professor, Precision Medical Lab Center, Chaozhou Central Hospital, No. 99 Dongshan Road, Chaozhou 521000, Guangdong Province, China. 26395868@qq.com
Research Domain of This Article
Medical Laboratory Technology
Article-Type of This Article
Retrospective Cohort Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Cardiol. Sep 26, 2025; 17(9): 110278 Published online Sep 26, 2025. doi: 10.4330/wjc.v17.i9.110278
Correlation of APOE, SLCO1B1 and LPA KIV-2 gene polymorphisms with coronary heart disease in the Teochew population
Jia-Xin Xu, Ye Wu, Lin Zhang, Yong-Hao Wu, Chun-Lai Li, Fen Lin
Jia-Xin Xu, Lin Zhang, Yong-Hao Wu, Fen Lin, Precision Medical Lab Center, Chaozhou Central Hospital, Chaozhou 521000, Guangdong Province, China
Jia-Xin Xu, Department of Laboratory Medicine, Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou 510000, Guangdong Province, China
Ye Wu, Clinical Laboratory, Chaozhou Central Hospital, Chaozhou 521000, Guangdong Province, China
Chun-Lai Li, Department of Emergency, Chaozhou Central Hospital, Chaozhou 521000, Guangdong Province, China
Author contributions: Lin F conceived the study and revised the manuscript; Xu JX analysed the data, drafted the initial manuscript, and revised the manuscript; Wu Y participated in the sample and data collection; Zhang L and Wu YH collected the data, performed the molecular analysis, and carried out the initial analysis; Li CL performed the routine diagnosis and treatment for the patients in our study. All the authors commented on previous versions of the manuscript. All the authors read and approved the final manuscript.
Supported by Special Research Plan 2023 of Chaozhou, No. 202303GY05.
Institutional review board statement: The study was reviewed and approved by the Chaozhou Central Hospital Institutional Review Board (Approval No. 2023014).
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
STROBE statement: The authors have read the STROBE Statement-checklist of items, and the manuscript was prepared and revised according to the STROBE Statement-checklist of items.
Data sharing statement: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Fen Lin, Chief, Professor, Precision Medical Lab Center, Chaozhou Central Hospital, No. 99 Dongshan Road, Chaozhou 521000, Guangdong Province, China. 26395868@qq.com
Received: June 11, 2025 Revised: June 25, 2025 Accepted: August 11, 2025 Published online: September 26, 2025 Processing time: 99 Days and 1 Hours
Abstract
BACKGROUND
Coronary heart disease (CHD) is a prominent cause of mortality and disability worldwide. Like most complex diseases, the risk of CHD in individuals is regulated by the interaction between genetic factors and lifestyle. APOE and SLCO1B1 genetic polymorphisms and LPA KIV-2 copy number variation may influence the development and progression of CHD. Clarifying gene polymorphisms can guide clinical precision and prevention, thereby improving treatment outcomes.
AIM
To investigate the influence of APOE and SLCO1B1 gene polymorphisms, as well as LPA KIV-2 copy number variation on CHD in the Teochew population.
METHODS
A total of 324 patients with CHD and 143 control participants were involved in this study. Single nucleotide polymorphisms rs429358 and rs7412 in the APOE gene, and rs2306283 and rs4149056 in the SLCO1B1 gene were analyzed via high-resolution melting curve analysis. Additionally, PCR was performed to detect KIV-2 copy number variations. Clinical risk factors and potential effects on CHD patients were subsequently assessed.
RESULTS
In the CHD group, the frequencies of APOE allele ε2, ε3, ε4 were 8.02%, 82.97%, and 9.10%, respectively. Compared to the control groups (13.29%, 79.37%, and 7.34%, respectively), the ε2 allele frequency showed a significant difference (8.02% vs 13.29%, P = 0.012). SLCO1B1 allele frequencies in the CHD group were not significantly different from those in the control group (*1a: 26.69% vs 25.52%, *1b: 61.17% vs 65.38%, *5: 0.15% vs 0.35%, *15: 11.83% vs 8.74%). The number of copies of the KIV-2 gene was significantly lower in the CHD group when compared to controls (23.35 ± 8.78 vs 27.21 ± 9.48; P < 0.01). Logistic regression analysis revealed that sex, age, hypertension, diabetes, smoking, the ε2 allele and KIV-2 copy number were factors influencing the presence of CHD.
CONCLUSION
In the Teochew population, the APOE ε2 allele and a higher KIV-2 copy number were associated with a reduced risk of CHD. In contrast, the APOE ε4 allele and SLCO1B1 gene were not associated with CHD.
Core Tip: In this study of 324 patients with coronary heart disease (CHD) and 143 control participants, we analysed polymorphisms in the APOE and SLCO1B1 genes, as well as LPA KIV-2 copy number variation in the Teochew population of southern China. We confirmed that both APOE ε2 allele and higher KIV-2 copy number are associated with a reduced risk of CHD in the Teochew population, whereas neither the APOE ε4 allele nor SLCO1B1 gene showed any association.
