Sá-Oliveira JA, Vieira Geraldo M, Marques M, Luiz RM, Krasinski Cestari F, Nascimento Lima I, De Souza TC, Zarpelon-Schutz AC, Teixeira KN. Bioactivity of dressings based on platelet-rich plasma and Platelet-rich fibrin for tissue regeneration in animal model. World J Biol Chem 2025; 16(1): 98515 [PMID: 40070854 DOI: 10.4331/wjbc.v16.i1.98515]
Corresponding Author of This Article
Kádima Nayara Teixeira, PhD, Professor, Researcher, Campus Toledo, Universidade Federal do Paraná, Max Planck 3796, Toledo 85919-899, Paraná, Brazil. kadimateixeira@ufpr.br
Research Domain of This Article
Immunology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Biol Chem. Mar 5, 2025; 16(1): 98515 Published online Mar 5, 2025. doi: 10.4331/wjbc.v16.i1.98515
Bioactivity of dressings based on platelet-rich plasma and Platelet-rich fibrin for tissue regeneration in animal model
João Abel Sá-Oliveira, Monique Vieira Geraldo, Milena Marques, Rafael Messias Luiz, Filipe Krasinski Cestari, Ingrid Nascimento Lima, Thayná Cristine De Souza, Ana Carla Zarpelon-Schutz, Kádima Nayara Teixeira
João Abel Sá-Oliveira, Kádima Nayara Teixeira, Programa Multicêntrico de Pós-graduação em Bioquímica e Biologia Molecular - Setor Palotina, Universidade Federal do Paraná, Palotina 85950-000, Paraná, Brazil
Monique Vieira Geraldo, Milena Marques, Rafael Messias Luiz, Ingrid Nascimento Lima, Ana Carla Zarpelon-Schutz, Kádima Nayara Teixeira, Campus Toledo, Universidade Federal do Paraná, Toledo 85919-899, Paraná, Brazil
Filipe Krasinski Cestari, Patologia, Laboratório Prevenção Patologia, Cascavel 85810-040, Paraná, Brazil
Thayná Cristine De Souza, Medicina-Campus Toledo, UFPR - Universidade Federal do Paraná, Toledo 85919-899, Paraná, Brazil
Ana Carla Zarpelon-Schutz, Programa de Pós-graduação em Biotecnologia-Setor Palotina, Universidade Federal do Paraná, Palotina 85950-000, Paraná, Brazil
Author contributions: Sá-Oliveira JA, Geraldo MV, Marques M and Luiz RM performed the in vivo experiments; Lima IN and De Souza TC carried out the pilot tests; Sá-Oliveira JA performed histology; Sá-Oliveira JA and Cestari FK analyze histology; Sá-Oliveira JA, Zarpelon-Schutz AC and Teixeira KN wrote manuscript; Zarpelon-Schutz AC and Teixeira KN interpreted the data, performed the critical analysis of the results and coordinated the study. All authors approved the final version of the manuscript.
Institutional review board statement: The Institutional Review Board declares knowledge about the study.
Institutional animal care and use committee statement: The Institutional Animal Care and Use Committee declares knowledge and approval about the study.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The authors declare that all data are public and can be shared upon reasonable request.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Kádima Nayara Teixeira, PhD, Professor, Researcher, Campus Toledo, Universidade Federal do Paraná, Max Planck 3796, Toledo 85919-899, Paraná, Brazil. kadimateixeira@ufpr.br
Received: June 28, 2024 Revised: October 14, 2024 Accepted: December 5, 2024 Published online: March 5, 2025 Processing time: 249 Days and 19.4 Hours
Abstract
BACKGROUND
Skin wounds are common injuries that affect quality of life and incur high costs. A considerable portion of healthcare resources in Western countries is allocated to wound treatment, mainly using mechanical, biological, or artificial dressings. Biological and artificial dressings, such as hydrogels, are preferred for their biocompatibility. Platelet concentrates, such as platelet-rich plasma (PRP) and platelet-rich fibrin (PRF), stand out for accelerating tissue repair and minimizing risks of allergies and rejection. This study developed PRF and PRP-based dressings to treat skin wounds in an animal model, evaluating their functionality and efficiency in accelerating the tissue repair process.
AIM
To develop wound dressings based on platelet concentrates and evaluating their efficiency in treating skin wounds in Wistar rats
METHODS
Wistar rats, both male and female, were subjected to the creation of a skin wound, distributed into groups (n = 64/group), and treated with Carbopol (negative control); PRP + Carbopol; PRF + Carbopol; or PRF + CaCl2 + Carbopol, on days zero (D0), D3, D7, D14, and D21. PRP and PRF were obtained only from male rats. On D3, D7, D14, and D21, the wounds were analyzed for area, contraction rate, and histopathology of the tissue repair process.
RESULTS
The PRF-based dressing was more effective in accelerating wound closure early in the tissue repair process (up to D7), while PRF + CaCl2 seemed to delay the process, as wound closure was not complete by D21. Regarding macroscopic parameters, animals treated with PRF + CaCl2 showed significantly more crusting (necrosis) early in the repair process (D3). In terms of histopathological parameters, the PRF group exhibited significant collagenization at the later stages of the repair process (D14 and D21). By D21, fibroblast proliferation and inflammatory infiltration were higher in the PRP group. Animals treated with PRF + CaCl2 experienced a more pronounced inflammatory response up to D7, which diminished from D14 onwards.
CONCLUSION
The PRF-based dressing was effective in accelerating the closure of cutaneous wounds in Wistar rats early in the process and in aiding tissue repair at the later stages.
Core Tip: This study compares the effects of dressings made from platelet concentrates platelet-rich fibrin (PRF), platelet-rich plasma, and PRF activated with CaCl2 - on accelerating the tissue repair process and closing cutaneous wounds in Wistar rats. The results indicated that the PRF dressing accelerated wound closure and tissue repair.
