Genetically engineered mouse models in gastric precancerous lesions research

Figure 1 Working principle of clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats-associated protein 9. The figures were created using the Figdraw platform. Non-homologous end joining: It directly ligates the broken DNA ends together, often resulting in small insertions or deletions. Homology-directed repair: Provide template DNA containing the sequence to be inserted to achieve gene knock-in, tagging or mutation correction. HDR: Homology-directed repair; NHEJ: Non-homologous end joining; sgDNA: Single-guide DNA; cas: Clustered regularly interspaced short palindromic repeats-associated protein; DSBs: DNA double-strand breaks.

Figure 2 Working principle of cyclization recombinase/locus of X-over P1 system. The figures were created using the Figdraw platform. Cyclization recombinase recombinase cuts the DNA at the locus of X-over P1 sites and then recombines the remaining DNA, ultimately resulting in the deletion of the target gene between the locus of X-over P1 sites. LoxP: Locus of X-over P1; Cre: Cyclization recombinase.