Luo X, Tan C, Tao F, Xu CY, Zheng ZH, Pang Q, He XA, Cao JQ, Duan JY. Differences in metabolic improvement after metabolic surgery are linked to the gut microbiota in non-obese diabetic rats. World J Gastrointest Surg 2023; 15(7): 1304-1316 [PMID: 37555105 DOI: 10.4240/wjgs.v15.i7.1304]
Corresponding Author of This Article
Jin-Yuan Duan, Doctor, Department of General Surgery, The First Affiliated Hospital of Nanchang University, No. 1519 Dongyue Avenue, Nanchang 330006, Jiangxi Province, China. duanjy2022@outlook.com
Research Domain of This Article
Endocrinology & Metabolism
Article-Type of This Article
Basic Study
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This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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Luo X, Tan C, Tao F, Xu CY, Zheng ZH, Pang Q, He XA, Cao JQ, Duan JY. Differences in metabolic improvement after metabolic surgery are linked to the gut microbiota in non-obese diabetic rats. World J Gastrointest Surg 2023; 15(7): 1304-1316 [PMID: 37555105 DOI: 10.4240/wjgs.v15.i7.1304]
Xin Luo, Fang Tao, Chi-Ying Xu, Jin-Yuan Duan, Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China
Cai Tan, Department of Women’s Health, Jiangxi Maternal and Child Health Hospital, Nanchang 330006, Jiangxi Province, China
Zhi-Hua Zheng, Qiang Pang, Xiang-An He, Jia-Qing Cao, Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China
Author contributions: Luo X and Tan C are co-first authors who have made significant contributions to this article; Luo X, Tan C, Cao JQ and Duan JY contributed to the conception of the study; Luo X, Tan C, Zheng ZH and Qiang P performed the experiment; Luo X, Tan C and Duan JY contributed significantly to analysis and manuscript preparation; Luo X, Tao F, Xue CY, Zheng ZH, Qiang P, He XA, Cao JQ and Duan JY helped perform the analysis with constructive discussions; Luo X, Tan C and Duan JY performed the data analyses and wrote the manuscript; All authors read and approved the final manuscript.
Supported bythe National Natural Science Foundation of China, No. 81760156, 81960154 and 82060161; and the Natural Science Foundation of Jiangxi Province, No. 2018ACB21040, 20203BBGL73185, and 20212BAB206020; and Foundation of Health commission of Jiangxi Province, No. SKJP220225830.
Institutional animal care and use committee statement: The animal experiments were conducted according to the Nanchang University Guide to Animal Experiments.
Conflict-of-interest statement: All authors declare that there are no potential conflicts of interest.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at duanjy2022@outlook.com.
ARRIVE guidelines statement: The authors have read the ARRIVE Guidelines, and the manuscript was prepared and revised according to the ARRIVE Guidelines.
Corresponding author: Jin-Yuan Duan, Doctor, Department of General Surgery, The First Affiliated Hospital of Nanchang University, No. 1519 Dongyue Avenue, Nanchang 330006, Jiangxi Province, China. duanjy2022@outlook.com
Received: January 30, 2023 Peer-review started: January 30, 2023 First decision: March 24, 2023 Revised: April 6, 2023 Accepted: April 21, 2023 Article in press: April 21, 2023 Published online: July 27, 2023 Processing time: 172 Days and 0.3 Hours
ARTICLE HIGHLIGHTS
Research background
The effectiveness of weight loss surgery is closely related to the gut microbiome, and many studies are exploring its mechanisms.
Research motivation
This study focused on the different effectiveness and gut microbiome differences of different weight loss surgeries to determine their key mechanisms through the different changes between the two surgeries.
Research objectives
The study aimed to explore the mechanisms behind the effectiveness of weight loss surgery and investigate non-surgical interventions that could improve metabolism.
Research methods
We performed sleeve gastrectomy (SG), distal small intestine bypass (DSIB) or sham surgery in nonobese rats with diabetes induced by 60 mg/kg streptozotocin (STZ-DM).
Research results
The group comparisons revealed that both SG and DSIB induced a reduction in body weight and significant improvements in glucose and lipid metabolism in the STZ-DM rats. Furthermore, DSIB exhibited a stronger glucose-lowering and lipid-reducing effect on STZ-DM rats than SG. 16S ribosomal RNA gene sequencing revealed that the gut abundance of some Lactobacillus spp. increased in both the SG and DSIB groups after surgery. However, the DSIB group exhibited a more pronounced increase in the gut abundance of Lactobacillus spp. compared to the SG group, with more Lactobacillus spp. types increased in the gut.
Research conclusions
The gut abundance of Lactobacillus was significantly correlated with the improvement in glycolipid metabolism and the change in serum FGF21 levels.
Research perspectives
The study found that the duodenal switch procedure has better metabolic improvement and more significant changes in gut microbiota. By examining the different effectiveness and gut microbiome changes of various weight loss surgeries, this study contributed to understanding their key mechanisms.
