Fan MY, Jiang QL, Cui MY, Zhao MQ, Wang JJ, Lu YY. Alteration of ascending colon mucosal microbiota in patients after cholecystectomy. World J Gastrointest Surg 2024; 16(8): 2436-2450 [PMID: 39220062 DOI: 10.4240/wjgs.v16.i8.2436]
Corresponding Author of This Article
Ying-Ying Lu, PhD, Associate Chief Physician, Department of Gastroenterology, Jiading Branch of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 800 Huangjiahuayuan Road, Shanghai 201803, China. yingying.lu1@shgh.cn
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Case Control Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Miao-Yan Fan, Qiao-Li Jiang, Ying-Ying Lu, Department of Gastroenterology, Jiading Branch of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201803, China
Miao-Yan Fan, Meng-Yan Cui, Meng-Qi Zhao, Jing-Jing Wang, Shanghai Key Laboratory of Pancreatic Diseases, Institute of Translational Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China
Meng-Yan Cui, Meng-Qi Zhao, Ying-Ying Lu, Department of Gastroenterology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China
Co-first authors: Miao-Yan Fan and Qiao-Li Jiang.
Author contributions: Fan MY and Jiang QL drafted the manuscript and collected patient samples and data; Cui MY and Zhao MQ performed the 16S rRNA sequencing data analysis; Wang JJ provided guidance for sample processing methodology; Lu YY was involved in the study design and critical revision of the manuscript; and all authors have read and approved the final manuscript.
Supported bythe Shanghai Natural Science Foundation Project, No. 22ZR1453500; and Jiading District Health Commission Scientific Project, No. 2023-KY-01.
Institutional review board statement: This study was reviewed and approved by Shanghai General Hospital’s Ethical Committee [approval No. (2023)252].
Informed consent statement: The written informed consent was obtained from all the patients before sample collection.
Conflict-of-interest statement: The authors declare that they have no competing interests.
Data sharing statement: No additional data are available.
STROBE statement: The authors have read the STROBE Statement—checklist of items, and the manuscript was prepared and revised according to the STROBE Statement—checklist of items.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Ying-Ying Lu, PhD, Associate Chief Physician, Department of Gastroenterology, Jiading Branch of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 800 Huangjiahuayuan Road, Shanghai 201803, China. yingying.lu1@shgh.cn
Received: April 5, 2024 Revised: May 23, 2024 Accepted: July 3, 2024 Published online: August 27, 2024 Processing time: 133 Days and 9.1 Hours
Abstract
BACKGROUND
Cholecystectomy is a successful treatment option for gallstones, although the incidence of colorectal cancer (CRC) has notably increased in post-cholecystectomy (PC) patients. However, it remains uncertain whether the altered mucosal microbiota in the ascending colon is related.
AIM
To investigate the potential correlation between gut microbiota and the surgical procedure of cholecystectomy.
METHODS
In total, 30 PC patients and 28 healthy controls underwent colonoscopies to collect mucosal biopsy samples. PC patients were divided based on their clinical features. Then, 16S-rRNA gene sequencing was used to analyze the amplicon, alpha diversity, beta diversity, and composition of the bacterial communities. Additionally, the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) database, sourced from the Kyoto Encyclopedia of Genes and Genomes, was used to predict the functional capabilities of the bacteria.
RESULTS
PC patients were comparable with healthy controls. However, PC patients older than 60 years had a distinct composition compared to those under 60 years old. Bacteroidetes richness was considerably higher at the phylum level in PC patients. Bacteroides, Parabacteroides, and Bilophila were more abundant in the PC group than in the control group. Furthermore, PC patients exhibited greater enrichment in metabolic pathways, specifically those related to lipopolysaccharide biosynthesis and vancomycin group antibiotic production, than controls.
CONCLUSION
This study indicated that the mucosal microbiota in PC patients was altered, perhaps offering new perspectives on the treatment possibilities for CRC and diarrhea following cholecystectomy.
Core Tip: Cholecystectomy is an effective treatment for gallstones, which may change the gut microbiota and relate to colorectal cancer (CRC). To investigate the potential correlation between gut microbiota and cholecystectomy, we performed 16S rRNA sequencing in ascending colon mucosa in post-cholecystectomy patients and healthy controls. We found some altered species that may affect the bile acid metabolism and have a relationship with CRC. Therefore, intestinal flora altered after cholecystectomy, which may be a new target to attenuate related diseases after cholecystectomy.
