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Goegebeur S, Everaert K, Radon P, Wells J, Löwa N, Coene A, Wiekhorst F, Leliaert J. Estimating the hysteresis loss in magnetic nanoparticles by magnetic particle spectroscopy. Phys Med Biol 2025; 70:105009. [PMID: 40273942 DOI: 10.1088/1361-6560/add07e] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/08/2024] [Accepted: 04/24/2025] [Indexed: 04/26/2025]
Abstract
Objective.Magnetic fluid hyperthermia is a promising adjuvant cancer therapy presently approaching clinical application. The therapeutic effect stems from the heat produced by magnetic nanoparticles (MNPs) administered to the tumor site and exposed to an AC magnetic field applied from outside the body. The objective of our study is to improve the integration of magnetic particle imaging (MPI) and hyperthermia as a theranostic application by allowing a real-time monitoring of local heat generation.Approach.The area of the dynamic hysteresis loop of the MNP is a measure of the heat produced by the MNP. However, depending on the specifics of the measurements, an accurate determination of the dynamic hysteresis loop of MNPs by conventional magnetic particle spectroscopy (MPS) can be hindered due to missing information of the first harmonic. The method presented in this work provides a solution to this problem by extracting the area of the hysteresis loop from measured MPS spectra through the reconstruction of the first harmonic.Main results.The method was tested on three distinct commercial MNP systems and found to be in good agreement with hysteresis loops directly obtained through AC magnetometry, confirming the method's reliability.Significance.This advancement enables accurate real-time monitoring of the energy dissipated as heat by the particles during MPS measurements and thus directly contributes to the development of MPI-guided hyperthermia.
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Affiliation(s)
- Samuel Goegebeur
- Department of Solid State Sciences, Ghent University, Belgium, Krijgslaan 281/S1, 9000 Gent, Belgium
| | - Katrijn Everaert
- Department of Solid State Sciences, Ghent University, Belgium, Krijgslaan 281/S1, 9000 Gent, Belgium
- Metrology for Magnetic Nanoparticles, Physikalisch-Technische Bundesanstalt, Germany, Abbestrasse 2-12, 10587 Berlin, Germany
- Department of Physics, University of Maryland, College Park, MD 20742, United States of America
| | - Patricia Radon
- Metrology for Magnetic Nanoparticles, Physikalisch-Technische Bundesanstalt, Germany, Abbestrasse 2-12, 10587 Berlin, Germany
| | - James Wells
- Metrology for Magnetic Nanoparticles, Physikalisch-Technische Bundesanstalt, Germany, Abbestrasse 2-12, 10587 Berlin, Germany
| | - Norbert Löwa
- Metrology for Magnetic Nanoparticles, Physikalisch-Technische Bundesanstalt, Germany, Abbestrasse 2-12, 10587 Berlin, Germany
| | - Annelies Coene
- Department of Electromechanical, Systems and Metal Engineering, Ghent University, Belgium, Technologiepark Zwijnaarde 131, 9052 Zwijnaarde, Belgium
| | - Frank Wiekhorst
- Metrology for Magnetic Nanoparticles, Physikalisch-Technische Bundesanstalt, Germany, Abbestrasse 2-12, 10587 Berlin, Germany
| | - Jonathan Leliaert
- Department of Solid State Sciences, Ghent University, Belgium, Krijgslaan 281/S1, 9000 Gent, Belgium
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Toyomasu Y, Mochiki E, Ito T, Ishiguro T, Suzuki O, Kumagai Y, Ishibashi K, Saeki H, Shirabe K, Ishida H. Gastric Emptying is Accelerated in Patients With Gastric Tube Reconstruction Following Laparoscopic Proximal Gastrectomy. Surg Laparosc Endosc Percutan Tech 2022; 32:683-687. [PMID: 36223321 DOI: 10.1097/sle.0000000000001106] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/02/2022] [Accepted: 08/25/2022] [Indexed: 06/16/2023]
Abstract
BACKGROUND Laparoscopic proximal gastrectomy (LPG) is an attractive option for the treatment of early gastric cancer in the upper third of the stomach. No optimal method of reconstruction after LPG has been established because of problems associated with postoperative reflux. Gastric tube reconstruction, a type of esophagogastrostomy, is a simple procedure, but it is associated with a high frequency of reflux esophagitis (RE). We investigated the relationship between RE and gastric emptying, along with nutritional parameters. SUBJECTS AND METHODS We compared gastric emptying in patients who had undergone curative LPG with gastric tube reconstruction for gastric cancer with that of patients after total gastrectomy (TG), distal gastrectomy (DG) and of healthy volunteers and patients after DG. The LPG group was divided into an RE LPG-RE (+) group and a non-reflux esophagitis (non-RE) an LPG-RE (-) group, and we compared gastric emptying and indices of nutrition, such as body weight and laboratory findings, between those among LPG-RE (+), LPG-RE (-), and TG groups. RESULTS The time lag between ingestion and peak 13 CO 2 expiration (T lag) in the healthy volunteer group was significantly shorter in the LPG group longer than those in the healthy volunteer LPG group and TG group. The T lag was significantly shorter in the RE LPG-RE (+) group than in the non-RE LPG-RE (-) group. The percentage change in body weight percentage in the non-RE LPG-RE (-) group was significantly larger than that in the RE LPG-RE (+) group at 12 months after surgery. Both the serum albumin and hemoglobin levels in the non-RE LPG-RE (-) tended to be preserved compared with those in the RE LPG-RE (+) group and TG group. CONCLUSIONS Gastric emptying was accelerated after LPG, and was associated with RE. Our data suggest that RE could be associated with body weight loss after LPG.
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Affiliation(s)
- Yoshitaka Toyomasu
- Department of Digestive Tract and General Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Saitama
- Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan
| | - Erito Mochiki
- Department of Digestive Tract and General Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Saitama
| | - Tetsuya Ito
- Department of Digestive Tract and General Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Saitama
| | - Toru Ishiguro
- Department of Digestive Tract and General Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Saitama
| | - Okihide Suzuki
- Department of Digestive Tract and General Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Saitama
| | - Youichi Kumagai
- Department of Digestive Tract and General Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Saitama
| | - Keiichiro Ishibashi
- Department of Digestive Tract and General Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Saitama
| | - Hiroshi Saeki
- Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan
| | - Ken Shirabe
- Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan
| | - Hideyuki Ishida
- Department of Digestive Tract and General Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Saitama
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Soares G, Pinto L, Liebl M, Biasotti G, Prospero A, Stoppa E, Bakuzis A, Baffa O, Wiekhorst F, Miranda JRA. Quantitative imaging of magnetic nanoparticles in an unshielded environment using a large AC susceptibility array. J Biol Eng 2022; 16:25. [PMID: 36221096 PMCID: PMC9552418 DOI: 10.1186/s13036-022-00305-9] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/02/2022] [Accepted: 09/26/2022] [Indexed: 11/29/2022] Open
Abstract
Background Non-invasive magnetic imaging techniques are necessary to assist magnetic nanoparticles in biomedical applications, mainly detecting their distribution inside the body. In Alternating Current Biosusceptometry (ACB), the magnetic nanoparticle's magnetization response under an oscillating magnetic field, which is applied through an excitation coil, is detected with a balanced detection coil system. Results We built a Multi-Channel ACB system (MC-ACB) containing nineteen pick-up coils and obtained 2D quantitative images of magnetic nanoparticle distributions by solving an inverse problem. We reconstructed the magnetic nanoparticles spatial distributions in a field of view of 14 × 14 cm2 with a spatial resolution of 2.0 cm and sensitivity in the milligram scale. A correlation coefficient between quantitative reconstructed and nominal magnetic nanoparticle distributions above 0.6 was found for all measurements. Conclusion Besides other interesting features such as sufficient large field of view dimension for mice and rat studies, portability, and the ability to assess the quantitative magnetic nanoparticles distributions in real-time, the MC-ACB system is a promising tool for quantitative imaging of magnetic nanoparticles distributions in real-time, offering an affordable setup for easy access in clinical or laboratory environments.
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Affiliation(s)
- Guilherme Soares
- Physikalisch-Technische Bundesanstalt, Abbestraße 2-12, 10587, Berlin, Germany. .,Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, 18618-689, Brazil.
| | - Leonardo Pinto
- Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, 18618-689, Brazil
| | - Maik Liebl
- Physikalisch-Technische Bundesanstalt, Abbestraße 2-12, 10587, Berlin, Germany
| | - Gabriel Biasotti
- Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, 18618-689, Brazil
| | - Andre Prospero
- Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, 18618-689, Brazil
| | - Erick Stoppa
- Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, 18618-689, Brazil
| | - Andris Bakuzis
- Institute of Physics, Federal University of Goiás, Goiânia, 74690-900, Brazil
| | - Oswaldo Baffa
- Faculty of Philosophy, Sciences and Letters at Ribeirão Preto, University of São Paulo, Ribeirão Preto, Sao Paulo, 14040-900, Brazil
| | - Frank Wiekhorst
- Physikalisch-Technische Bundesanstalt, Abbestraße 2-12, 10587, Berlin, Germany
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Ding F, Guo R, Cui ZY, Hu H, Zhao G. Clinical application and research progress of extracellular slow wave recording in the gastrointestinal tract. World J Gastrointest Surg 2022; 14:544-555. [PMID: 35979419 PMCID: PMC9258241 DOI: 10.4240/wjgs.v14.i6.544] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/06/2022] [Revised: 03/21/2022] [Accepted: 05/17/2022] [Indexed: 02/06/2023] Open
Abstract
The physiological function of the gastrointestinal (GI) tract is based on the slow wave generated and transmitted by the interstitial cells of Cajal. Extracellular myoelectric recording techniques are often used to record the characteristics and propagation of slow wave and analyze the models of slow wave transmission under physiological and pathological conditions to further explore the mechanism of GI dysfunction. This article reviews the application and research progress of electromyography, bioelectromagnetic technology, and high-resolution mapping in animal and clinical experiments, summarizes the clinical application of GI electrical stimulation therapy, and reviews the electrophysiological research in the biliary system.
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Affiliation(s)
- Fan Ding
- Center of Gallbladder Disease, East Hospital of Tongji University, Shanghai 200120, China
- Institute of Gallstone Disease, Tongji University School of Medicine, Shanghai 200331, China
| | - Run Guo
- Department of Ultrasonography, East Hospital of Tongji University, Shanghai 200120, China
| | - Zheng-Yu Cui
- Department of Internal Medicine of Traditional Chinese Medicine, East Hospital of Tongji University, Shanghai 200120, China
| | - Hai Hu
- Center of Gallbladder Disease, East Hospital of Tongji University, Shanghai 200120, China
- Institute of Gallstone Disease, Tongji University School of Medicine, Shanghai 200331, China
| | - Gang Zhao
- Center of Gallbladder Disease, East Hospital of Tongji University, Shanghai 200120, China
- Institute of Gallstone Disease, Tongji University School of Medicine, Shanghai 200331, China
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Bezerra AT, Pinto LA, Rodrigues DS, Bittencourt GN, Mancera PFDA, Miranda JRDA. Classification of gastric emptying and orocaecal transit through artificial neural networks. MATHEMATICAL BIOSCIENCES AND ENGINEERING : MBE 2021; 18:9511-9524. [PMID: 34814356 DOI: 10.3934/mbe.2021467] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/13/2023]
Abstract
Classical quantification of gastric emptying (GE) and orocaecal transit (OCT) based on half-life time T$ _{50} $, mean gastric emptying time (MGET), orocaecal transit time (OCTT) or mean caecum arrival time (MCAT) can lead to misconceptions when analyzing irregularly or noisy data. We show that this is the case for gastrointestinal transit of control and of diabetic rats. Addressing this limitation, we present an artificial neural network (ANN) as an alternative tool capable of discriminating between control and diabetic rats through GE and OCT analysis. Our data were obtained via biological experiments using the alternate current biosusceptometry (ACB) method. The GE results are quantified by T$ _{50} $ and MGET, while the OCT is quantified by OCTT and MCAT. Other than these classical metrics, we employ a supervised training to classify between control and diabetes groups, accessing sensitivity, specificity, $ f_1 $ score, and AUROC from the ANN. For GE, the ANN sensitivity is 88%, its specificity is 83%, and its $ f_1 $ score is 88%. For OCT, the ANN sensitivity is 100%, its specificity is 75%, and its $ f_1 $ score is 85%. The area under the receiver operator curve (AUROC) from both GE and OCT data is about 0.9 in both training and validation, while the AUCs for classical metrics are 0.8 or less. These results show that the supervised training and the binary classification of the ANN was successful. Classical metrics based on statistical moments and ROC curve analyses led to contradictions, but our ANN performs as a reliable tool to evaluate the complete profile of the curves, leading to a classification of similar curves that are barely distinguished using statistical moments or ROC curves. The reported ANN provides an alert that the use of classical metrics can lead to physiological misunderstandings in gastrointestinal transit processes. This ANN capability of discriminating diseases in GE and OCT processes can be further explored and tested in other applications.
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Affiliation(s)
- Anibal Thiago Bezerra
- Institute of Exact Sciences, Federal University of Alfenas-MG (UNIFAL-MG), Alfenas-MG 37133-840, Brazil
| | - Leonardo Antonio Pinto
- Institute of Biosciences, São Paulo State University (UNESP), Botucatu-SP 18618-689, Brazil
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Prospero AG, Pinto LA, Matos RVR, Soares GA, Oliveira RB, Mascarenhas S, Miranda JRDA. New device for active gastric mechanical stimulation. Neurogastroenterol Motil 2021; 33:e14169. [PMID: 33969918 DOI: 10.1111/nmo.14169] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Revised: 04/16/2021] [Indexed: 02/08/2023]
Abstract
BACKGROUND Gastroparesis is a chronic stomach disorder and effective treatment is the aim of different strategies. Alternative therapies consist of an electrical stimulation of the stomach to evoke a response in the gastric activity. We present the development and in vivo application of an electromagnet system to induce a mechanical stimulus in the stomach aiming for gastric contractile responses. METHODS The electromagnet system consisted of an implantable magnet and an external drive coil. We implanted the magnet at the greater curvature of the gastric body in rats. We applied an alternating current to the drive coils, inducing mechanical stimulation of the gastric wall. We measured the gastric contraction activity and gastric electrical activity in response to the stimulus using AC biosusceptometry and electrogastrography. Moreover, we used the phenol red to evaluate the stimulus effects on gastrointestinal transit. KEY RESULTS The stimulus increased the spectral intensity and signal-to-noise ratio significantly of gastric contraction activity and gastric electrical activity. Furthermore, we found a lower phenol red retention in the stomach in rats without stimulus. No significant differences were found in frequency and root mean square amplitude. CONCLUSIONS & INFERENCES We developed a new simple electromagnet system that evoked a contraction and gastric electrical response using a mechanical stimulus and decreased gastric emptying time. The system is an accessible tool and may contribute to gastroparesis studies in animals.
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Affiliation(s)
- Andre Gonçalves Prospero
- Departamento de Biofísica e Farmacologia, Laboratório de Biomagnetismo, UNESP/São Paulo State University, Botucatu, Brazil
| | - Leonardo Antonio Pinto
- Departamento de Biofísica e Farmacologia, Laboratório de Biomagnetismo, UNESP/São Paulo State University, Botucatu, Brazil
| | - Ronaldo Vitor Reis Matos
- Departamento de Biofísica e Farmacologia, Laboratório de Biomagnetismo, UNESP/São Paulo State University, Botucatu, Brazil
| | - Guilherme Augusto Soares
- Departamento de Biofísica e Farmacologia, Laboratório de Biomagnetismo, UNESP/São Paulo State University, Botucatu, Brazil
| | - Ricardo Brandt Oliveira
- Faculdade de Medicina de Ribeirão Preto, USP/University of São Paulo, Ribeirão Preto, Brazil
| | | | - José Ricardo de Arruda Miranda
- Departamento de Biofísica e Farmacologia, Laboratório de Biomagnetismo, UNESP/São Paulo State University, Botucatu, Brazil
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Pinto L, Soares G, Próspero A, Stoppa E, Biasotti G, Paixão F, Santos A, Oliveira R, Miranda J. An easy and low-cost biomagnetic methodology to study regional gastrointestinal transit in rats. ACTA ACUST UNITED AC 2021; 66:405-412. [PMID: 33544465 DOI: 10.1515/bmt-2020-0202] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/03/2020] [Accepted: 01/19/2021] [Indexed: 12/12/2022]
Abstract
The identification of gastrointestinal (GI) motility disorders requires the evaluation of regional GI transit, and the development of alternative methodologies in animals has a significant impact on translational approaches. Therefore, the purpose of this study was to validate an easy and low-cost methodology (alternate current biosusceptometry - ACB) for the assessment of regional GI transit in rats through images. Rats were fed a test meal containing magnetic tracer and phenol red, and GI segments (stomach, proximal, medial and distal small intestine, and cecum) were collected to assess tracer's retention at distinct times after ingestion (0, 60, 120, 240, and 360 min). Images were obtained by scanning the segments, and phenol red concentration was determined by the sample's absorbance. The temporal retention profile, geometric center, gastric emptying, and cecum arrival were evaluated. The correlation coefficient between methods was 0.802, and the temporal retention of each segment was successfully assessed. GI parameters yielded comparable results between methods, and ACB images presented advantages as the possibility to visualize intrasegmental tracer distribution and the automated scan of the segments. The imaging approach provided a reliable assessment of several parameters simultaneously and may serve as an accurate and sensitive approach for regional GI research in rats.
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Affiliation(s)
- Leonardo Pinto
- Department of Biophysics and Pharmacology, São Paulo State University, Biosciences Institute of Botucatu, Botucatu, Brazil
| | - Guilherme Soares
- Department of Biophysics and Pharmacology, São Paulo State University, Biosciences Institute of Botucatu, Botucatu, Brazil
| | - André Próspero
- Department of Biophysics and Pharmacology, São Paulo State University, Biosciences Institute of Botucatu, Botucatu, Brazil
| | - Erick Stoppa
- Department of Biophysics and Pharmacology, São Paulo State University, Biosciences Institute of Botucatu, Botucatu, Brazil
| | - Gabriel Biasotti
- Department of Biophysics and Pharmacology, São Paulo State University, Biosciences Institute of Botucatu, Botucatu, Brazil
| | - Fabiano Paixão
- Science and Technology Institute, Federal University of São Paulo, São José dos Campos, Brazil
| | - Armênio Santos
- Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil
| | - Ricardo Oliveira
- Ribeirão Preto Medical School, São Paulo University, Ribeirão Preto, Brazil
| | - José Miranda
- Department of Biophysics and Pharmacology, São Paulo State University, Biosciences Institute of Botucatu, Botucatu, Brazil
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Karpavičius A, Coene A, Bender P, Leliaert J. Advanced analysis of magnetic nanoflower measurements to leverage their use in biomedicine. NANOSCALE ADVANCES 2021; 3:1633-1645. [PMID: 36132562 PMCID: PMC9417518 DOI: 10.1039/d0na00966k] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/18/2020] [Accepted: 02/04/2021] [Indexed: 05/08/2023]
Abstract
Magnetic nanoparticles are an important asset in many biomedical applications ranging from the local heating of tumours to targeted drug delivery towards diseased sites. Recently, magnetic nanoflowers showed a remarkable heating performance in hyperthermia experiments thanks to their complex structure leading to a broad range of magnetic dynamics. To grasp their full potential and to better understand the origin of this unexpected heating performance, we propose the use of Kaczmarz' algorithm in interpreting magnetic characterisation measurements. It has the advantage that no a priori assumptions need to be made on the particle size distribution, contrasting current magnetic interpretation methods that often assume a lognormal size distribution. Both approaches are compared on DC magnetometry, magnetorelaxometry and AC susceptibility characterisation measurements of the nanoflowers. We report that the lognormal distribution parameters vary significantly between data sets, whereas Kaczmarz' approach achieves a consistent and accurate characterisation for all measurement sets. Additionally, we introduce a methodology to use Kaczmarz' approach on distinct measurement data sets simultaneously. It has the advantage that the strengths of the individual characterisation techniques are combined and their weaknesses reduced, further improving characterisation accuracy. Our findings are important for biomedical applications as Kaczmarz' algorithm allows to pinpoint multiple, smaller peaks in the nanostructure's size distribution compared to the monomodal lognormal distribution. The smaller peaks permit to fine-tune biomedical applications with respect to these peaks to e.g. boost heating or to reduce blurring effects in images. Furthermore, the Kaczmarz algorithm allows for a standardised data analysis for a broad range of magnetic nanoparticle samples. Thus, our approach can improve the safety and efficiency of biomedical applications of magnetic nanoparticles, paving the way towards their clinical use.
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Affiliation(s)
| | - Annelies Coene
- Department of Electromechanical, Systems and Metal Engineering, Ghent University Zwijnaarde Belgium
- Cancer Research Institute Ghent Ghent Belgium
| | - Philipp Bender
- Department of Physics and Materials Science, University of Luxembourg Luxembourg Grand Duchy of Luxembourg
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Calabresi MFF, Tanimoto A, Próspero AG, Mello FPF, Soares G, Di Stasi LC, Miranda JRA. Changes in colonic contractility in response to inflammatory bowel disease: Long-term assessment in a model of TNBS-induced inflammation in rats. Life Sci 2019; 236:116833. [PMID: 31491456 DOI: 10.1016/j.lfs.2019.116833] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/14/2019] [Revised: 08/22/2019] [Accepted: 09/02/2019] [Indexed: 02/06/2023]
Abstract
AIMS Inflammatory bowel disease is a chronic relapsing inflammation that affects the gastrointestinal tract, causing changes in colonic motility. The evolution of these changes is not completely understood and possibly related to symptoms that appear in different degrees of the intestinal inflammation. Therefore, our aim is evaluate during 14 days of assessment aspects of colonic contractility using 2,4,6-trinitrobenzenesulfonic acid (TNBS) model of inflammation in rats and associate the inflammatory process with colonic motility. METHODS Contractility and inflammatory parameters were assessed in the same animal in six different moments: before intestinal inflammation induction, 2, 5, 8, 11, and 14 days after induction. The mechanical activity was determined by alternating current biosusceptometry (ACB) and subdivided into rhythmic propagating ripples (RPR) and rhythmic propulsive motor complexes (RPMC). We assessed inflammation by determining myeloperoxidase activity in feces. RESULTS Transient and permanent changes were observed in colonic motility as a function of the inflammatory process evaluated through myeloperoxidase activity. We identified two contraction profiles: RPR and RPMC. The microscopic analysis demonstrated a depth of damage caused by an injury that was associated with changes in motility. CONCLUSIONS We implemented a robust and adequate (specific) signal processing to quantify two measured colonic frequency patterns. Thus, we performed a detailed temporal analysis of the consequences of TNBS-induced inflammation on colonic motility in rats. Our approach enables further long-term assessments in the same animal with different mechanisms and duration of injury, remission, treatments and their motor consequences.
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Affiliation(s)
- Marcos F F Calabresi
- Laboratory of Biomagnetism, Department of Physics and Biophysics, Bioscience Institute, São Paulo State University, Botucatu, São Paulo, Brazil.
| | - Alexandre Tanimoto
- Laboratory of Phytomedicines, Pharmacology and Biotechnology, Department of Pharmacology, Bioscience Institute, São Paulo State University, Botucatu, São Paulo, Brazil.
| | - André G Próspero
- Laboratory of Biomagnetism, Department of Physics and Biophysics, Bioscience Institute, São Paulo State University, Botucatu, São Paulo, Brazil.
| | - Fabio P F Mello
- Laboratory of Biomagnetism, Department of Physics and Biophysics, Bioscience Institute, São Paulo State University, Botucatu, São Paulo, Brazil
| | - Guilherme Soares
- Laboratory of Biomagnetism, Department of Physics and Biophysics, Bioscience Institute, São Paulo State University, Botucatu, São Paulo, Brazil
| | - Luiz C Di Stasi
- Laboratory of Phytomedicines, Pharmacology and Biotechnology, Department of Pharmacology, Bioscience Institute, São Paulo State University, Botucatu, São Paulo, Brazil.
| | - José R A Miranda
- Laboratory of Biomagnetism, Department of Physics and Biophysics, Bioscience Institute, São Paulo State University, Botucatu, São Paulo, Brazil.
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Gastrointestinal effects of ivermectin treatment in rats infected with Strongyloides venezuelensis. Acta Trop 2019; 194:69-77. [PMID: 30914242 DOI: 10.1016/j.actatropica.2019.03.024] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/11/2018] [Revised: 03/14/2019] [Accepted: 03/22/2019] [Indexed: 12/27/2022]
Abstract
We aimed to evaluate the effects of ivermectin treatment on gastrointestinal morphology and function after Strongyloides venezuelensis infection. Male rats composed Control (C), Parasitized (Sv), Ivermectin (IVM) and Parasitized and treated with Ivermectin (Sv/IVM) groups. IVM and Sv/IVM groups were subdivided according to IVM: single dose of 200 μg/kg (IVM1 and Sv/IVM1) or three repeated doses of 200 μg/kg at 24 h intervals (IVM3 and Sv/IVM3). First dose of IVM was administered after peak of infection. Eggs per gram (EPG), mean gastric emptying time (MGET), mean cecum arrival time (MCAT) and mean small intestinal transit time (MSITT) were evaluated. Measurements were performed before drug and at peak of infection, first day post peak of infection and 30 days post infection. Same time intervals were simulated for uninfected animals. Number of recovered worms and intestinal morphometry were also rated. Data were analyzed by ANOVA and correlated by Dunnett and Pearson (p < 0.05). Sv/IVM1 and Sv/IVM3 showed reduction of EPG and worms, although only group SV/IVM3 eradicate them. Hastened gastric emptying and slowed intestinal transit provoked by S. venezuelensis infection can be reverted by a single administration of IVM after peak of infection, even without total parasite elimination. Although three consecutive doses of IVM were more efficient to eradicate the parasite, drug administration impaired gastrointestinal function and morphology. IVM alone affected gastrointestinal parameters in uninfected animals for prolonged periods, especially in high doses. In control, there were strong negative correlations between MSITT and muscle layers. Strongyloides venezuelensis infection abolishes such correlations. Longitudinal muscle was thinner in IVM3 and Sv/IVM3 groups and circular thicker in Sv group. Revisiting the action of traditional drugs broadens knowledge in the parasite-host interface and may result in the development of more accurate therapeutic strategies.
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Soares GA, Prospero AG, Calabresi MF, Rodrigues DS, Simoes LG, Quini CC, Matos RR, Pinto LA, Sousa-Junior AA, Bakuzis AF, Mancera PA, Miranda JRA. Multichannel AC Biosusceptometry System to Map Biodistribution and Assess the Pharmacokinetic Profile of Magnetic Nanoparticles by Imaging. IEEE Trans Nanobioscience 2019; 18:456-462. [PMID: 30998477 DOI: 10.1109/tnb.2019.2912073] [Citation(s) in RCA: 15] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/07/2022]
Abstract
In this paper, the application of a technique to evaluate in vivo biodistribution of magnetic nanoparticles (MNP) is addressed: the Multichannel AC Biosusceptometry System (MC-ACB). It allows real-time assessment of magnetic nanoparticles in both bloodstream clearance and liver accumulation, where a complex network of inter-related cells is responsible for MNP uptake. Based on the acquired MC-ACB images, we propose a mathematical model which helps to understand the distribution and accumulation pharmacokinetics of MNP. The MC-ACB showed a high time resolution to detect and monitor MNP, providing sequential images over the particle biodistribution. Utilizing the MC-ACB instrument, we assessed regions corresponding to the heart and liver, and we determined the MNP transfer rates between the bloodstream and the liver. The pharmacokinetic model resulted in having a strong correlation with the experimental data, suggesting that the MC-ACB is a valuable and accessible imaging device to assess in vivo and real-time pharmacokinetic features of MNP.
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Hauschildt AT, Corá LA, Volpato GT, Sinzato YK, Damasceno DC, Américo MF. Mild diabetes: long-term effects on gastric motility evaluated in rats. Int J Exp Pathol 2018; 99:29-37. [PMID: 29479759 PMCID: PMC5917388 DOI: 10.1111/iep.12262] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/19/2017] [Accepted: 01/04/2018] [Indexed: 12/22/2022] Open
Abstract
Moderate hyperglycaemic levels seem to be related to abnormal gastric motility in diabetes mellitus. However, experimental models designed to evaluate the relationship between motility and diabetes over time are not yet well established. Our objective was to investigate the long-term effects of mild diabetes on gastric motility in rats. Newborn male rats received streptozotocin (mild diabetes groups - MD) or vehicle (control groups - C), and both groups were evaluated after 3 (C3 and MD3) and 6 months (C6 and MD6) postinduction. Mild diabetic animals (MD3 and MD6) showed moderately elevated blood glucose and decreased insulin levels compared with control (C3 and C6). Insulin secretion was enhanced in MD6 compared with MD3, most likely due to partial β-cell regeneration indicated by HOMA-β. In HOMA-IR, it was noticed that MD6 animals had impaired insulin response compared with MD3. Gastric emptying was faster, amplitude of contraction was stronger in MD6 compared with MD3, and in both groups, the differences were significant when compared with control animals. A significant abnormal rhythmic index was calculated for the mild diabetic groups, despite unchanged mean frequency of contraction. In conclusion, despite increased insulin levels over time, constant levels of moderate hyperglycaemia are also related to abnormal gastric motility and impairment of gastric function.
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Affiliation(s)
| | - Luciana A. Corá
- Alagoas State University of Health Sciences – UNCISALMaceió/ALBrazil
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Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE 2017; 13:1519-1529. [DOI: 10.1016/j.nano.2017.02.005] [Citation(s) in RCA: 30] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/22/2016] [Revised: 01/30/2017] [Accepted: 02/05/2017] [Indexed: 01/05/2023]
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Matos JF, Americo MF, Sinzato YK, Volpato GT, Corá LA, Calabresi MFF, Oliveira RB, Damasceno DC, Miranda JRA. Role of sex hormones in gastrointestinal motility in pregnant and non-pregnant rats. World J Gastroenterol 2016; 22:5761-5768. [PMID: 27433089 PMCID: PMC4932211 DOI: 10.3748/wjg.v22.i25.5761] [Citation(s) in RCA: 26] [Impact Index Per Article: 2.9] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/07/2016] [Revised: 05/07/2016] [Accepted: 06/02/2016] [Indexed: 02/06/2023] Open
Abstract
AIM: To correlate gastric contractility, gastrointestinal transit, and hormone levels in non-pregnant (estrous cycle) and pregnant rats using noninvasive techniques.
METHODS: Female rats (n = 23) were randomly divided into (1) non-pregnant, (contractility, n = 6; transit, n = 6); and (2) pregnant (contractility, n = 5; transit, n = 6). In each estrous cycle phase or at 0, 7, 14, and 20 d after the confirmation of pregnancy, gastrointestinal transit was recorded by AC biosusceptometry (ACB), and gastric contractility was recorded by ACB and electromyography. After each recording, blood samples were obtained for progesterone and estradiol determination.
RESULTS: In the estrous cycle, despite fluctuations of sex hormone levels, no significant changes in gastrointestinal motility were observed. Days 7 and 14 of pregnancy were characterized by significant changes in the frequency of contractions (3.90 ± 0.42 cpm and 3.60 ± 0.36 cpm vs 4.33 ± 0.25 cpm) and gastric emptying (168 ± 17 min and 165 ± 15 min vs 113 ± 15 min) compared with day 0. On these same days, progesterone levels significantly increased compared with control (54.23 ± 15.14 ng/mL and 129.96 ± 30.52 ng/mL vs 13.25 ± 6.31 ng/mL). On day 14, we observed the highest level of progesterone and the lowest level of estradiol compared with day 0 (44.3 ± 15.18 pg/mL vs 24.96 ± 5.96 pg/mL).
CONCLUSION: Gastrointestinal motility was unaffected by the estrous cycle. In our data, high progesterone and low estradiol levels can be associated with decreased contraction frequency and slow gastric emptying.
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