Lomatogonium rotatum extract alleviates diabetes mellitus induced by a high-fat, high-sugar diet and streptozotocin in rats

Table 1 Content of active compounds measured in the Lomatogonium rotatum extract

Active compound	Regression equation	R2	Linear range, μg	LR extract, mg/g dry mater
Swertiamarin	Y = 99.413X + 6.3517	1	1.953-9.826	91.10
Sweroside	Y = 198.81X + 0.8476	1	0.431-2.154	6.09
Hesperetin	Y = 437.89X + 0.0685	1	0.119-1.002	7.65
Coumarin	Y = 365.15X + 0.0103	1	0.118-0.590	3.04
1.7-dihydroxy-3.8-dimethoxyxanthone	Y = 214.16X + 2.3704	0.9999	0.098-0.494	29.28
1-hydroxy-2,3,5 trimethoxanthone	Y = 90.498X + 7.1204	0.9999	2.026-10.305	3.70

LR: Lomatogonium rotatum.

Table 2 Differential metabolites were determined by cross-comparison between different groups in rat serum samples

No.	Metabolites	Control group vs model group		Trend	P value	Model group vsLomatogonium rotatum		Trend	P value
		VIP	FC			VIP	FC
1	Marshrin	1.46	1.16	Decreased	0.016	1.59	0.68	Increased	0.034
2	Honyucitrin	1.67	1.23	Decreased	0.002	1.54	0.68	Increased	0.047
3	Isoliquiritigenin	1.60	1.75	Decreased	0.004	1.79	0.42	Increased	0.018
4	Pyridoxal	1.53	1.33	Decreased	0.029	1.73	1.47	Increased	0.055
5	1H-indole-2,3-dione	1.21	4.08	Decreased	0.019	1.17	0.34	Increased	0.039
6	Oxychlordane	1.41	1.10	Decreased	0.019	1.30	0.92	Increased	0.039
7	Phosphorylcholine	1.55	1.25	Decreased	0.009	1.26	0.84	Increased	0.045
8	Mevalonic acid-5P	1.32	2.16	Decreased	0.001	1.03	0.56	Increased	0.010
9	D-proline	1.37	1.36	Decreased	0.003	1.80	1.39	Increased	0.019
10	L-lysine	1.40	1.50	Decreased	0.006	1.71	2.42	Increased	0.002
11	Taurine	1.67	1.48	Decreased	0.017	1.61	1.59	Increased	0.006
12	Se-adenosylselenohomocysteine	1.25	1.50	Decreased	0.029	2.24	2.35	Increased	0.001
13	1-methyladenosine	1.47	1.46	Decreased	0.019	1.81	0.58	Increased	0.001
14	LysoPE[0:0/18:3(6Z,9Z,12Z)]	1.64	1.45	Decreased	0.003	1.80	0.63	Increased	0.002
15	PE[20:4(8Z,11Z,14Z,17Z)/P-16:0]	1.80	1.68	Decreased	0.001	1.70	0.72	Increased	0.001
16	Bakers yeast extract	1.51	1.17	Decreased	0.012	1.74	0.70	Increased	0.016
17	Ecgonine methyl ester	1.48	1.10	Decreased	0.013	1.23	0.92	Increased	0.033
18	Dihydrothy	1.66	0.79	Increased	0.013	1.59	0.79	Decreased	0.048
19	Pantothenic acid	1.78	0.65	Increased	0.028	1.97	0.61	Decreased	0.024
20	Aromadendrin 4’-methyl ether 7-rhamnoside	1.35	1.60	Increased	0.030	1.66	2.22	Decreased	0.007

The significance of potential metabolites in the serum of rats induced by Lomatogonium rotatum is shown in this table. FC: Fold change; VIP: Variable importance in projection.