Basic Study
Copyright ©The Author(s) 2021.
World J Diabetes. Apr 15, 2021; 12(4): 466-479
Published online Apr 15, 2021. doi: 10.4239/wjd.v12.i4.466
Table 1 Primer sequences of target genes and internal reference genes
Gene
Amplicon size (bp)
Forward primer (5'→3')
Reverse primer (5'→3')
β-actin150CCCATCTATGAGGGTTACGCTTTAATGTCACGCACGATTTC
Cyclin D1138TCAAGTGTGACCCGGACTGGACCAGCTTCTTCCTCCACTT
STAT3115GCAATACCATTGACCTGCCGAACGTGAGCGACTCAAACTG
RBP4131GCGAGGAAACGATGACCACTTGGGGTCACGAGAAAACACA
JAK2179ACAAGCAGGACGGGAAGGTCAATTGGGCCGTGACAGTTGC
Bcl2102GAGTACCTGAACCGGCATCTGAAATCAAACAGAGGTCGCA
Table 2 Comparison of indicators among each group
Index
Group NC (n = 20)
Group DM (n = 25)
Group DA (n = 25)
F
P value
Weight (kg)491.84 ± 80.82504.61 ± 63.16526.07 ± 85.661.1550.321
TG (mmol/L)0.65 ± 0.101.36 ± 0.17a1.82 ± 0.28a,b191.339< 0.001
LDL-c (mmol/L)0.35 ± 0.120.49 ± 0.12a0.57 ± 0.14a,b17.562< 0.001
HDL-c (mmol/L)1.07 ± 0.190.98 ± 0.200.71 ± 0.11a,b27.856< 0.001
TC (mmol/L)1.98 ± 0.392.23 ± 0.422.95 ± 0.50 a,b30.234< 0.001
FPG (mmol/L)5.42 ± 0.8213.37 ± 2.16a14.04 ± 2.40a125.463< 0.001
FINS (mU/L)9.84 ± 1.9914.42 ± 2.12a19.24 ± 3.17a,b77.990< 0.001
HbA1C (%)5.10 ± 0.819.95 ± 2.02a10.86 ± 1.65a78.800< 0.001
RBP4 (ng/mL)15.37 ± 2.0721.23 ± 2.70a32.28 ± 4.68a,b144.583< 0.001
AI0.58 ± 0.231.71 ± 0.71a3.31 ± 0.76a,b105.551< 0.001
HOMA-IR2.38 ± 0.628.49 ± 1.36a12.24 ± 2.82a,b149.994< 0.001
Table 3 Immunohistochemical analysis of Cyclin D1 and B-cell lymphoma-2 protein expression in all groups
Index
Group NC (%)
Group DM (%)
Group DA (%)
F
P value
Cyclin D16.84 ± 1.0310.24 ± 1.78a14.71 ± 2.26a,b15.0170.005
Bcl-27.25 ± 0.9610.44 ± 1.21a13.28 ± 2.04a,b12.510 0.007
Table 4 Correlation between serum retinol binding protein 4 and the other indicators in type 2 diabetes groups (n = 50)
Indicator
r
P value
Weight (kg)0.1210.401
TG (mmol/L)0.622< 0.001
LDL-c (mmol/L)0.3950.005
HDL-c (mmol/L)-0.566< 0.001
TC (mmol/L)0.586< 0.001
AI0.716< 0.001
FPG (mmol/L)0.1110.444
FINS (mU/L)0.556< 0.001
HOMA-IR0.541< 0.001
HbA1C (%)0.2840.045
JAK20.2390.094
p-JAK20.4330.002
STAT3-0.0400.781
p-STAT30.539< 0.001
Cyclin D10.476< 0.001
Bcl-20.3250.021
Table 5 Multivariable logistic regression in type 2 diabetes groups (n = 50)
Variable
β value
SE
χ2
OR
95%CI
P value
RBP40.9510.3387.9332.5891.335-5.0190.005
LDL-c5.2112.4214.63118.2531.592-39.5970.031
p-JAK22.0400.8665.5547.6931.410-41.9790.018
p-STAT32.7341.1875.30515.4021.503-57.7940.021
Constant-21.0077.9167.0410.0000.008