de Oliveira Lira A, de Brito Alves JL, Pinheiro Fernandes M, Vasconcelos D, Santana DF, da Costa-Silva JH, Morio B, Góis Leandro C, Pirola L. Maternal low protein diet induces persistent expression changes in metabolic genes in male rats. World J Diabetes 2020; 11(5): 182-192 [PMID: 32477454 DOI: 10.4239/wjd.v11.i5.182]
Corresponding Author of This Article
Luciano Pirola, PhD, Senior Researcher, Carmen (Cardiology, Metabolism and Nutrition) Laboratory, INSERM U1060, Lyon-1 University, South Lyon Medical Faculty Sector 2, CENS ELI-2D building, 165 Chemin du grand Revoyet, Pierre Benite 69310, France. luciano.pirola@univ-lyon1.fr
Research Domain of This Article
Endocrinology & Metabolism
Article-Type of This Article
Basic Study
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de Oliveira Lira A, de Brito Alves JL, Pinheiro Fernandes M, Vasconcelos D, Santana DF, da Costa-Silva JH, Morio B, Góis Leandro C, Pirola L. Maternal low protein diet induces persistent expression changes in metabolic genes in male rats. World J Diabetes 2020; 11(5): 182-192 [PMID: 32477454 DOI: 10.4239/wjd.v11.i5.182]
World J Diabetes. May 15, 2020; 11(5): 182-192 Published online May 15, 2020. doi: 10.4239/wjd.v11.i5.182
Maternal low protein diet induces persistent expression changes in metabolic genes in male rats
Allan de Oliveira Lira, José Luiz de Brito Alves, Mariana Pinheiro Fernandes, Diogo Vasconcelos, David Filipe Santana, João Henrique da Costa-Silva, Béatrice Morio, Carol Góis Leandro, Luciano Pirola
Allan de Oliveira Lira, Diogo Vasconcelos, David Filipe Santana, Carol Góis Leandro, Department of Nutrition, Federal University of Pernambuco, Vitoria de Santo Antão, Pernambuco 55608680, Brazil
José Luiz de Brito Alves, Department of Nutrition, Federal University of Paraíba, Joao Pessoa 74556060, Brazil
Mariana Pinheiro Fernandes, Henrique da Costa-Silva, Luciano Pirola, Department of Physical Education and Sport Sciences, Federal University of Pernambuco, Vitoria de Santo Antão, Pernambuco 55608680, Brazil
Béatrice Morio, Luciano Pirola, Carmen (Cardiology, Metabolism and Nutrition) Laboratory, INSERM U1060, Lyon-1 University, South Lyon Medical Faculty, Pierre Benite 69310, France
Author contributions: de Oliveira Lira A performed experiments and data analysis and contributed to writing; de Brito Alves JL performed experiments and data analysis; Pinheiro Fernandes M performed experiments; Vasconcelos D performed experiments; Santana DF performed experiments; da Costa-Silva JH supervised the project and contributed to writing; Morio B contributed to writing; Leandro CG supervised the project, performed data analysis and contributed to writing; Pirola L supervised the project, wrote the final manuscript version and managed the submission process.
Supported bythe CAPES/COFECUB, No. 797-14; the National Council for Research – Brazil, No. 477915/2012-4.
Institutional animal care and use committee statement: The experimental protocol was approved by the Ethical Committee of the Biological Sciences Centre (protocol 23076 062778/2014-38), Federal University of Pernambuco, Brazil.
Conflict-of-interest statement: The authors declare no competing financial interests.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Corresponding author: Luciano Pirola, PhD, Senior Researcher, Carmen (Cardiology, Metabolism and Nutrition) Laboratory, INSERM U1060, Lyon-1 University, South Lyon Medical Faculty Sector 2, CENS ELI-2D building, 165 Chemin du grand Revoyet, Pierre Benite 69310, France. luciano.pirola@univ-lyon1.fr
Received: December 12, 2019 Peer-review started: December 12, 2019 First decision: January 6, 2020 Revised: March 16, 2020 Accepted: March 30, 2020 Article in press: March 30, 2020 Published online: May 15, 2020 Processing time: 144 Days and 20.7 Hours
Core Tip
Core tip: Perinatal exposure to a poor nutritional environment predisposes to metabolic disease. Here, the expression of metabolism-related genes in adipose tissue and liver was investigated. We evaluated the alterations in gene expression of glycolytic/Krebs cycle genes, adipogenic and lipolytic genes in adipose tissue of offspring rats at 30 d/90 d of age, exposed to maternal low protein diet throughout gestation/lactation. We also evaluated expression of liver transcription factors and gluconeogenic genes. Persistent gene alterations were observed that may account for the metabolic adaptations in response to maternal low protein diet, highlighting the occurrence of persistent transcriptional defects as a consequence of perinatal malnutrition.
