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Tang W, Chen T, Luo Z, Chen J. Association between urinary volatile organic compound metabolites and sarcopenia in the US general population: a cross-sectional NHANES study from 2011 to 2018. Sci Rep 2025; 15:10735. [PMID: 40155648 PMCID: PMC11953313 DOI: 10.1038/s41598-025-94622-w] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/05/2024] [Accepted: 03/17/2025] [Indexed: 04/01/2025] Open
Abstract
Volatile organic compound (VOC) is a prevalent form of pollutant that has been linked to various human ailments, yet their connection to sarcopenia remains uncertain. This study seeks to examine the potential association between exposure to mixtures of metabolites of volatile organic compounds (mVOCs) and sarcopenia, while also investigating the potential mediating effects of oxidative stress and inflammation. Data from the 2011-2018 National Health and Nutrition Examination Survey (NHANES) were utilized for the analysis of the relationship between mVOCs and sarcopenia through logistic regression. The least absolute shrinkage and selection operator (LASSO) regression model was employed to identify key mVOCs, while the quantile-g computation model (qgcomp) and bayesian kernel machine regression (BKMR) models were utilized to examine the association between mVOC mixtures and sarcopenia. Potential mediating factors were explored through mediating analysis. Of the 2908 participants included in the study, 246 individuals (8.5%) were found to have sarcopenia. Logistic regression analysis revealed that five urinary VOC metabolites were positively correlated with an increased risk of sarcopenia. The key mVOCs identified through the LASSO method were further analyzed using qgcomp, which showed a 47% average increase in the risk of sarcopenia when exposed to a mixture of mVOCs (OR = 1.47, 95% CI 1.14-1.91). Four mVOCs components (DHBMA, 3HPMA, ATCA and 3,4MHA) have the largest weight. The BKMR results further confirm this joint association. Furthermore, Mediation analysis revealed that inflammation and oxidative stress mediate the relationship between exposure to mVOCs and sarcopenia. In conclusion, our study provides evidence suggesting that VOC exposure is linked to a heightened risk of sarcopenia, with inflammation and oxidative stress potentially serving as mediators in this relationship. It is recommended that additional cohort studies be conducted to validate these findings.
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Affiliation(s)
- Wei Tang
- Department of Nephrology, The Second Xiangya Hospital of Central South University, Changsha, 410011, China
- Hunan Key Laboratory of Kidney Disease and Blood Purification, The Second Xiangya Hospital of Central South University, Changsha, 410011, China
| | - Tuotuo Chen
- Department of Emergency Medicine, The Second Xiangya Hospital of Central South University, Changsha, 410011, China
| | - Zixuan Luo
- Department of Nephrology, The Second Xiangya Hospital of Central South University, Changsha, 410011, China
- Hunan Key Laboratory of Kidney Disease and Blood Purification, The Second Xiangya Hospital of Central South University, Changsha, 410011, China
| | - Junxiang Chen
- Department of Nephrology, The Second Xiangya Hospital of Central South University, Changsha, 410011, China.
- Hunan Key Laboratory of Kidney Disease and Blood Purification, The Second Xiangya Hospital of Central South University, Changsha, 410011, China.
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Duan X, Chen Z, Liao J, Wen M, Yue Y, Liu L, Li X, Long L. The association analysis between exposure to volatile organic compounds and fatty liver disease in US Adults. Dig Liver Dis 2025; 57:535-541. [PMID: 39426902 DOI: 10.1016/j.dld.2024.09.027] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/02/2024] [Revised: 07/15/2024] [Accepted: 09/27/2024] [Indexed: 10/21/2024]
Abstract
BACKGROUND Limited epidemiological research has explored the associations between ambient volatile organic compounds (VOCs) and fatty liver disease (FLD). This study aimed to explore the associations between VOCs and FLD and liver function biomarkers. We obtained urinary concentrations of VOCs metabolites from NHANES. METHODS Weighted logistic regression models were employed to investigate the relationships between VOCs and FLD risk, including alcoholic FLD (AFLD) and non-alcoholic FLD (NAFLD). The associations of VOCs and liver function biomarkers were also investigated using weighted linear regression. RESULTS Among the 2050 participants, 774 were classified as having FLD. After adjustment, each log-transformed SD increase in N-Acetyl-S-(2-carboxyethyl)-l-cysteine (CEMA), 2-Aminothiazoline-4-carboxylicacid (ATCA), and trans-trans-muconic-acid (MUCA) had a OR (95%CI) of 1.30 (1.06-1.61; P-trend=0.014), 1.34 (1.12-1.61; P-trend=0.002), and 1.22 (1.01-1.47; P-trend=0.035), respectively. ATCA and MUCA were associated with higher risks of NAFLD (OR=1.47, 95%CI: 1.20-1.79, and OR=1.26, 95%CI: 1.02-1.56, respectively). VOCs were positively associated with gamma glutamyl transaminase (GGT) and C-reactive protein (CRP), while inversely associated with albumin, total protein and alanine aminotransferase (ALT) (P < 0.05). CONCLUSIONS Urinary metabolites of VOCs have been found to be strongly correlated with a higher risk of FLD and NFALD, and impaired liver function. These novel findings merit further prospective studies to comprehend the effect of VOCs on liver diseases.
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Affiliation(s)
- Xiaoxia Duan
- Department of Epidemiology and Health Statistics, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China; Laboratory of Child and Adolescent Psychiatry, Mental Health Center, West China Hospital, Sichuan University, Chengdu, China
| | - Zhenhua Chen
- Department of Microbiology Laboratory, Chengdu Municipal Center for Disease Control and Pevention, Chengdu, China
| | - Juan Liao
- Department of Gastroenterology, West China School of public health and West China Forth Hospital, Sichuan University, Chengdu, China
| | | | - Yong Yue
- Chengdu Hi-Tech Development Zone Center for Disease Control and Prevention, Chengdu, China
| | - Li Liu
- Department of Epidemiology and Biostatistics, School of Public Health, Guangdong Pharmaceutical University, Guangzhou, China
| | - Xiaojing Li
- Chengdu Jinjiang Center for Disease Control and Prevention, Chengdu, China
| | - Lu Long
- Department of Epidemiology and Health Statistics, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.
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Zhang J, Li R, Wang K, Xu T, He Y, Han T, Lin X, Jin L. Relation of volatile organic compounds to renal function in American adolescents: three statistical models. INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH 2025:1-12. [PMID: 39890440 DOI: 10.1080/09603123.2025.2461106] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/18/2024] [Accepted: 01/28/2025] [Indexed: 02/03/2025]
Abstract
This study was conducted to evaluate the relationship between 17 urinary metabolites of volatile organic compounds (mVOCs) in adolescents and renal function parameters (estimated glomerular filtration rate (eGFR), albumin-to-creatinine ratio (ACR), urinary albumin, serum uric acid (SUA), and blood urea nitrogen (BUN)). In adjusted generalised linear models (GLM), mVOCs were positively correlated with eGFR, urinary albumin, and BUN, and mVOCs were negatively correlated with ACR and SUA. Weighted Quartile Sum (WQS) index correlated with eGFR [β(95%CI): 0.040 (0.028, 0.052)], urine albumin [β(95%CI): 0.275 (0.203, 0.622)], SUA [β(95%CI): 0.040 (0.025, 0.055)] and BUN [β(95%CI): 0.102 (0.082, 0.122)]. In Bayesian Kernel Machine Regression (BKMR) model, total compound effect was positively correlated with eGFR, positive associations were observed in high concentration of the mixture with urine albumin and ACR. Findings suggest that single and mixed exposures to mVOCs may affect renal parameters in adolescents.
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Affiliation(s)
- Jiaqi Zhang
- Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, Jilin, China
| | - Runhong Li
- Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, Jilin, China
| | - Kaiyuan Wang
- Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, Jilin, China
| | - Tong Xu
- Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, Jilin, China
| | - Yue He
- Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, Jilin, China
| | - Tianyang Han
- Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, Jilin, China
| | - Xinli Lin
- Department of Child and Adolescent Health, School of Public Health, Jilin University, Changchun, Jilin, China
| | - Lina Jin
- Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, Jilin, China
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Sun M, Li X, Geng M, Zhou X, Zhang Z, Nie H, Xia N, Huang G, Wang X, Zhang H. Associations of coke oven emission exposure with pulmonary function, blood pressure, blood cell parameters, and biochemical indices in coking workers: a cross-sectional pilot study. ENVIRONMENTAL SCIENCE. PROCESSES & IMPACTS 2025; 27:91-103. [PMID: 39582442 DOI: 10.1039/d4em00306c] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/26/2024]
Abstract
Background and objective: Coke oven emissions (COEs) are formed in the process of coking production, mainly composed of polycyclic aromatic hydrocarbons (PAHs) and benzene; however, the health impacts of COE exposure in coking workers are not fully clear so far. We aimed to explore the associations of occupational COE exposure with pulmonary function, blood pressure, blood cell parameters, and blood biochemical indices, and to bolster health surveillance and disease prevention and control in coking workers. Methods: We investigated 566 coking workers at a large state-owned enterprise coking plant in Taiyuan, Shanxi, China, measured the concentrations of plasma 16 PAHs and urinary phenol, assessed the health outcomes including pulmonary function, blood pressure, the levels of peripheral hematologic parameters and biochemical indices, and examined the associations of PAH and phenol concentrations with the health outcomes using multiple linear regressions, least absolute shrinkage and selection operator regression (LASSO), and Bayesian kernel machine regression (BKMR). Results: After adjustment for confounders, plasma ∑15PAH concentration was significantly associated with increases in hemoglobin (HGB) and triglyceride (TG) levels in coking workers, and urinary phenol concentration was significantly associated with increases in the diastolic blood pressure (DBP) level, and decreases in platelet (PLT) count. When phenol concentration and PAH concentration were simultaneously included in the multiple linear regression model, both of them were associated with the level of HGB. LASSO and BKMR indicated that the PAHs with four rings and above contributed to the HGB level. Conclusion: PAH exposure could damage hematological parameters and blood lipids, and benzene exposure could increase blood pressure and decrease PLT count.
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Affiliation(s)
- Min Sun
- Department of Environmental Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, Shanxi, China.
- Center for Ecological Public Health Security of Yellow River Basin, Shanxi Medical University, 56 Xinjian South Road, Taiyuan 030001, China
- Key Laboratory of Coal Environmental Pathogenicity and Prevention Shanxi Medical University, Ministry of Education, Taiyuan, China
| | - Xin Li
- Radiological Health Department of TISCO General Hospital, Taiyuan 030003, Shanxi, China
| | - Mengmeng Geng
- Department of Environmental Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, Shanxi, China.
- Center for Ecological Public Health Security of Yellow River Basin, Shanxi Medical University, 56 Xinjian South Road, Taiyuan 030001, China
- Key Laboratory of Coal Environmental Pathogenicity and Prevention Shanxi Medical University, Ministry of Education, Taiyuan, China
| | - Xiaoling Zhou
- Department of Environmental Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, Shanxi, China.
- Center for Ecological Public Health Security of Yellow River Basin, Shanxi Medical University, 56 Xinjian South Road, Taiyuan 030001, China
- Key Laboratory of Coal Environmental Pathogenicity and Prevention Shanxi Medical University, Ministry of Education, Taiyuan, China
| | - Zhiyan Zhang
- Department of Environmental Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, Shanxi, China.
- Center for Ecological Public Health Security of Yellow River Basin, Shanxi Medical University, 56 Xinjian South Road, Taiyuan 030001, China
- Key Laboratory of Coal Environmental Pathogenicity and Prevention Shanxi Medical University, Ministry of Education, Taiyuan, China
| | - Huixiang Nie
- Radiological Health Department of TISCO General Hospital, Taiyuan 030003, Shanxi, China
| | - Na Xia
- Department of Environmental Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, Shanxi, China.
- Center for Ecological Public Health Security of Yellow River Basin, Shanxi Medical University, 56 Xinjian South Road, Taiyuan 030001, China
- Key Laboratory of Coal Environmental Pathogenicity and Prevention Shanxi Medical University, Ministry of Education, Taiyuan, China
| | - Guoshun Huang
- Health Examination Department of TISCO General Hospital, Taiyuan 030003, Shanxi, China
| | - Xuhong Wang
- Health Examination Department of TISCO General Hospital, Taiyuan 030003, Shanxi, China
| | - Hongmei Zhang
- Department of Environmental Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, Shanxi, China.
- Center for Ecological Public Health Security of Yellow River Basin, Shanxi Medical University, 56 Xinjian South Road, Taiyuan 030001, China
- Key Laboratory of Coal Environmental Pathogenicity and Prevention Shanxi Medical University, Ministry of Education, Taiyuan, China
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Jiang W, Wu W, Zhang K, Liu L, Yan B. Mediating role of immune cells in association between volatile organic compounds and periodontitis: NHANES 2011-2014. J Periodontol 2024. [PMID: 39692522 DOI: 10.1002/jper.24-0150] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/28/2024] [Revised: 10/31/2024] [Accepted: 11/17/2024] [Indexed: 12/19/2024]
Abstract
BACKGROUND The relationship between humans and volatile organic compounds (VOCs) is a persistent concern due to their widespread sources and high evaporation rates. However, there is currently limited direct evidence linking VOC exposure to the development of periodontitis. METHODS This cross-sectional study analyzed 1525 participants and 21 urinary VOCs in the National Health and Nutrition Examination Survey (NHANES) from 2011 to 2014, aiming to investigate the relationship between periodontitis risk, assessed by attachment loss (AL) and probing depth (PD) and individual VOCs using logistic regression, quantile regression, and subgroup analysis. Weighted quantile sum analysis (WQS) and subgroup analysis were utilized to evaluate whether VOC mixtures were associated with periodontitis risk. Multiple linear regression models were used to examine the association between VOC co-exposure and peripheral immune cell counts. A mediation analysis was performed to evaluate whether peripheral immune cells are involved in the effect of VOC co-exposure on periodontitis prevalence. RESULTS Urinary levels of 2-aminothiazoline-4-carboxylic acid, mandelic acid, and N-acetyl-S-(4-hydroxy-2-butenyl)-L-cysteine were positively associated with the risk of periodontitis after adjusting for all covariates. The WQS models demonstrated a positive correlation between the mixture of VOCs and the risk of periodontitis, wherein 2-aminothiazoline-4-carboxylic acid emerged as the most important contributor. The mediation analysis suggested that monocytes may play a role in the observed association between VOC co-exposure and the prevalence of periodontitis. CONCLUSIONS Exposure to VOCs is associated with a greater prevalence of periodontitis. Monocytes' mediating role plays a crucial function in the association between the risk of periodontitis and co-exposure to VOCs. PLAIN LANGUAGE SUMMARY Volatile organic compounds (VOCs) are chemicals that evaporate quickly and are found all around us-from paints to cleaning products. Understanding how these compounds affect our health is crucial, especially regarding conditions like periodontitis, a common oral chronic inflammatory disease. In our study, we looked at urine samples from 1525 people who participated in a national health survey between 2011 and 2014 to find out if there is an association between VOC exposure and the risk of developing periodontitis. We found that certain chemicals in the urine, which show VOC exposure, were indeed associated with a greater risk of the disease. We further investigated the collective impact of these VOCs on the risk of periodontitis, revealing that certain chemicals exert a more significant influence than their counterparts. Additionally, our research hints at a potential role for monocytes in the interplay between VOCs and the risk of periodontitis. Our data suggest that exposure to VOCs could be associated with a greater likelihood of periodontitis, with monocytes potentially playing a role in this relationship. This study helps us better understand the potential health impacts of daily chemical exposure and underscores the importance of investigating further how our environment affects our health.
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Affiliation(s)
- Wenxiu Jiang
- Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
- State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing, China
- Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
| | - Wei Wu
- State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing, China
- Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
- Department of Dental Implantology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
| | - Kejia Zhang
- Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
- State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing, China
- Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
| | - Luwei Liu
- Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
- State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing, China
- Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
| | - Bin Yan
- Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
- State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing, China
- Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
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Ogbodo JO, Egba SI, Ogbodo CG, Onwurah IE, Njoku OU. Effects of exposure to volatile organic compounds (VOCs) content from paint on automobile paint workers in Nsukka, South Eastern Nigeria. Heliyon 2024; 10:e37015. [PMID: 39286128 PMCID: PMC11402925 DOI: 10.1016/j.heliyon.2024.e37015] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/08/2024] [Revised: 08/01/2024] [Accepted: 08/26/2024] [Indexed: 09/19/2024] Open
Abstract
Background Volatile organic compounds (VOCs) fume in the workplace can act as an inducing agent to many health disorders. Objectives This work investigated the effects of exposure to VOCs content from paint on the automobile paint workers in South Eastern Nigeria. Methods A total of fifty (50) respondent participated in the study. Following the completion of informed consent form and well-structured questionnaire, blood samples were drawn and used for biochemical analysis. Results The results of the haematological analysis showed a significant (p < 0.05) increase in white blood cell (WBC) cluster of differentiation 4 (CD4), and platelet (PLT), and a significant (p < 0.05) decrease in packed cell volume (PCV), hemoglobin (HB), lymphocytes (LYM) and eosinophil (EOS) of the exposed automobile paint workers compared to the control (unexposed workers). Results also showed significant (p < 0.05) increase in liver marker indices; alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin (TB) and albumin (ALB) as well as significant (p < 0.05) decrease in Alkaline phosphatase (ALP), total protein (TP), direct bilirubin (DB). There was significant (p < 0.05) increase in urea, creatinine, potassium (K+), uric acid and nitric oxide concentrations and decrease in sodium (Na+) and bicarbonate (HCO3-) Again, results showed significant increase in Glutathione (GSH), and Glutathione peroxidase (GPx) and significant (p < 0.05) decrease in Superoxide dismutase (SOD) and Catalase (CAT). The Malondialdehyde MDA concentration showed varied significant (p < 0.05) difference based on ages. There was significant (p < 0.05) increase in luteinizing hormone (LH) and Follicle stimulating hormone (FSH), and significant (p < 0.05) decrease in the Testosterone (TET) concentrations of the exposed automobile paint workers compared to the unexposed workers. Conclusions Result of this study suggests a toxic outcome due to exposure to VOCs in spray paint workers.
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Affiliation(s)
| | - Simeon Ikechukwu Egba
- Department of Biochemistry, Research and Extension, Kampala International University, Uganda
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Wang Y, Yu Y, Zhang X, Zhang H, Zhang Y, Wang S, Yin L. Combined association of urinary volatile organic compounds with chronic bronchitis and emphysema among adults in NHANES 2011-2014: The mediating role of inflammation. CHEMOSPHERE 2024; 361:141485. [PMID: 38438022 DOI: 10.1016/j.chemosphere.2024.141485] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/10/2023] [Revised: 01/26/2024] [Accepted: 02/15/2024] [Indexed: 03/06/2024]
Abstract
Evidence on the association of volatile organic compounds (VOCs) with chronic bronchitis (CB) and emphysema is spare and defective. To evaluate the relationship between urinary metabolites of VOCs (mVOCs) with CB and emphysema, and to identify the potential mVOC of paramount importance, data from NHANES 2011-2014 waves were utilized. Logistic regression was conducted to estimate the independent association of mVOCs with respiratory outcomes. Least absolute shrinkage and selection operator (LASSO) regression was performed to screen a parsimonious set of CB- and emphysema-relevant mVOCs that were used for further co-exposure analyses of weighted quantile sum (WQS) regression and Bayesian kernel machine regression (BKMR). Mediation analysis was employed to detect the mediating role of inflammatory makers in such associations. In single exposure analytic model, nine mVOCs were individually and positively associated with CB, while four mVOCs were with emphysema. In WQS regression, positive association between LASSO selected mVOCs and CB was identified (OR = 1.82, 95% CI: 1.25 to 2.69), and N-acetyl-S-(4-hydroxy-2-butenyl)-l-cysteine (MHBMA3) weighted the highest. Results from BKMR further validated such combined association and the significance of MHBMA3. As for emphysema, significantly positive overall trend of mVOCs was only observed in BKMR model and N-acetyl-S-(N-methylcarbamoyl)-l-cysteine (AMCC) contributed most to the mixed effect. White blood cell count (WBC) and lymphocyte number (LYM) were mediators in the positive pattern of mVOCs mixture with CB, while association between mVOCs mixture and emphysema was significantly mediated by LYM and segmented neutrophils num (NEO). This study demonstrated that exposure to VOCs was associated with CB and emphysema independently and combinedly, which might be partly speculated that VOCs were linked to activated inflammations. Our findings shed novel light on VOCs related respiratory illness, and provide a new basis for the contribution of certain VOCs to the risk of CB and emphysema, which has potential public health implications.
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Affiliation(s)
- Yucheng Wang
- Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China
| | - Yongquan Yu
- Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China
| | - Xiaoxuan Zhang
- Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China
| | - Hu Zhang
- Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China
| | - Ying Zhang
- Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China
| | - Shizhi Wang
- Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China
| | - Lihong Yin
- Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China.
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Choi YH, Lee JY, Moon KW. Exposure to volatile organic compounds and polycyclic aromatic hydrocarbons is associated with the risk of non-alcoholic fatty liver disease in Korean adolescents: Korea National Environmental Health Survey (KoNEHS) 2015-2017. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2023; 251:114508. [PMID: 36621033 DOI: 10.1016/j.ecoenv.2023.114508] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/19/2022] [Revised: 12/22/2022] [Accepted: 01/02/2023] [Indexed: 06/17/2023]
Abstract
Non-alcoholic fatty liver disease (NAFLD) is one of the most frequent liver diseases among adolescents. Several animal studies have suggested that volatile organic compounds (VOCs) and polycyclic aromatic hydrocarbons (PAHs) increase NAFLD risk. However, few epidemiological studies have confirmed the association between VOCs, PAHs and NAFLD in the general adolescent population. Therefore, we analyzed 798 adolescents from the Korean National Environmental Health Survey (KoNEHS), 2015-2017, to examine the associations of urinary metabolites of VOCs and PAHs with serum alanine aminotransferase (ALT) activity and NAFLD prevalence. We performed linear regression, logistic regression, and Bayesian kernel machine regression (BKMR) to evaluate the association of urinary VOCs and PAHs metabolites with ALT levels and NAFLD prevalence. After adjusting for all covariates, urinary benzylmercapturic acid and 2-hydroxyfluorene levels were found to increase ALT activity and NAFLD prevalence. Additionally, the BKMR analyses showed a significantly positive overall effect on ALT activity and NAFLD prevalence with urinary concentrations of VOCs and PAHs metabolites, with 2-hydroxyfluorene as the biggest contributor. Our study suggests that exposure to low-level VOCs and PAHs may have a detrimental effect on NAFLD risk in adolescents. Given the increasing prevalence of NAFLD in adolescents, future cohort studies are confirmed to comprehend the effect of these chemicals on NAFLD risk.
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Affiliation(s)
- Yun-Hee Choi
- Department of Health and Safety Convergence Science, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, South Korea; BK21 FOUR R&E Center for Learning Health System, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, South Korea
| | - Ju-Yeon Lee
- Department of Health and Safety Convergence Science, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, South Korea; BK21 FOUR R&E Center for Learning Health System, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, South Korea
| | - Kyong Whan Moon
- BK21 FOUR R&E Center for Learning Health System, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, South Korea; Department of Health and Environmental Science, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, South Korea.
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Wahlang B, Gripshover TC, Gao H, Krivokhizhina T, Keith RJ, Sithu ID, Rai SN, Bhatnagar A, McClain CJ, Srivastava S, Cave MC. Associations Between Residential Exposure to Volatile Organic Compounds and Liver Injury Markers. Toxicol Sci 2021; 185:50-63. [PMID: 34668566 PMCID: PMC8714366 DOI: 10.1093/toxsci/kfab119] [Citation(s) in RCA: 16] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/13/2022] Open
Abstract
Occupational exposures to volatile organic compounds (VOCs) have been associated with numerous health complications including steatohepatitis and liver cancer. However, the potential impact of environmental/residential VOC exposures on liver health and function is largely unknown. To address this knowledge gap, the objective of this cross-sectional study is to investigate associations between VOCs and liver injury biomarkers in community residents. Subjects were recruited from six Louisville neighborhoods, and informed consent was obtained. Exposure biomarkers included 16 creatinine-adjusted urinary metabolites corresponding to 12 parent VOCs. Serological disease biomarkers measured included cytokertain-18 (K18 M65 and M30), liver enzymes, and direct bilirubin. Associations between exposure and disease biomarkers were assessed using generalized linear models. Smoking status was confirmed through urinary cotinine levels. The population comprised of approximately 60% females and 40% males; White persons accounted 78% of the population; with more nonsmokers (n = 413) than smokers (n = 250). When compared with nonsmokers, males (45%) and Black persons (26%) were more likely to be smokers. In the overall population, metabolites of acrolein, acrylonitrile, acrylamide, 1,3-butadiene, crotonaldehyde, styrene, and xylene were positively associated with alkaline phosphatase. These associations persisted in smokers, with the exception of crotonaldehyde, and addition of N,N-dimethylformamide and propylene oxide metabolites. Although no positive associations were observed for K18 M30, the benzene metabolite was positively associated with bilirubin, irrespective of smoking status. Taken together, the results demonstrated that selected VOCs were positively associated with liver injury biomarkers. These findings will enable better risk assessment and identification of populations vulnerable to liver disease.
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Affiliation(s)
- Banrida Wahlang
- Superfund Research Center, the University of Louisville, Louisville, Kentucky 40202, USA
- Division of Gastroenterology, Hepatology & Nutrition, Department of Medicine, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
- The Center for Integrative Environmental Health Sciences, University of Louisville, Louisville, Kentucky 40202, USA
| | - Tyler C Gripshover
- Superfund Research Center, the University of Louisville, Louisville, Kentucky 40202, USA
- Department of Pharmacology & Toxicology, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
| | - Hong Gao
- Superfund Research Center, the University of Louisville, Louisville, Kentucky 40202, USA
- Envirome Institute, University of Louisville, Louisville, Kentucky 40202, USA
- Division of Environmental Medicine, Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
| | - Tatiana Krivokhizhina
- Superfund Research Center, the University of Louisville, Louisville, Kentucky 40202, USA
- Envirome Institute, University of Louisville, Louisville, Kentucky 40202, USA
- Division of Environmental Medicine, Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
| | - Rachel J Keith
- Superfund Research Center, the University of Louisville, Louisville, Kentucky 40202, USA
- Envirome Institute, University of Louisville, Louisville, Kentucky 40202, USA
- Division of Environmental Medicine, Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
| | - Israel D Sithu
- Superfund Research Center, the University of Louisville, Louisville, Kentucky 40202, USA
- Envirome Institute, University of Louisville, Louisville, Kentucky 40202, USA
- Division of Environmental Medicine, Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
| | - Shesh N Rai
- Superfund Research Center, the University of Louisville, Louisville, Kentucky 40202, USA
- The Center for Integrative Environmental Health Sciences, University of Louisville, Louisville, Kentucky 40202, USA
- Envirome Institute, University of Louisville, Louisville, Kentucky 40202, USA
- Department of Bioinformatics and Biostatistics, the School of Public Health and Information Sciences, the University of Louisville, Louisville, Kentucky 40202, USA
- Biostatistics and Bioinformatics Facility, James Graham Brown Cancer Center, Louisville, Kentucky 40202, USA
| | - Aruni Bhatnagar
- Superfund Research Center, the University of Louisville, Louisville, Kentucky 40202, USA
- The Center for Integrative Environmental Health Sciences, University of Louisville, Louisville, Kentucky 40202, USA
- Department of Pharmacology & Toxicology, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
- Envirome Institute, University of Louisville, Louisville, Kentucky 40202, USA
- Division of Environmental Medicine, Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
- Department of Biochemistry and Molecular Genetics, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
| | - Craig J McClain
- Superfund Research Center, the University of Louisville, Louisville, Kentucky 40202, USA
- Division of Gastroenterology, Hepatology & Nutrition, Department of Medicine, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
- The Center for Integrative Environmental Health Sciences, University of Louisville, Louisville, Kentucky 40202, USA
- Department of Pharmacology & Toxicology, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
- Envirome Institute, University of Louisville, Louisville, Kentucky 40202, USA
- Department of Biochemistry and Molecular Genetics, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
- The Hepatobiology and Toxicology Center, University of Louisville, Louisville, Kentucky 40202, USA
- Alcohol Research Center, University of Louisville, Louisville, Kentucky 40202, USA
| | - Sanjay Srivastava
- Superfund Research Center, the University of Louisville, Louisville, Kentucky 40202, USA
- Department of Pharmacology & Toxicology, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
- Envirome Institute, University of Louisville, Louisville, Kentucky 40202, USA
- Division of Environmental Medicine, Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
- Department of Biochemistry and Molecular Genetics, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
| | - Mathew C Cave
- Superfund Research Center, the University of Louisville, Louisville, Kentucky 40202, USA
- Division of Gastroenterology, Hepatology & Nutrition, Department of Medicine, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
- The Center for Integrative Environmental Health Sciences, University of Louisville, Louisville, Kentucky 40202, USA
- Department of Pharmacology & Toxicology, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
- Envirome Institute, University of Louisville, Louisville, Kentucky 40202, USA
- Department of Biochemistry and Molecular Genetics, the University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
- The Hepatobiology and Toxicology Center, University of Louisville, Louisville, Kentucky 40202, USA
- Alcohol Research Center, University of Louisville, Louisville, Kentucky 40202, USA
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10
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O'Dell CT, Boule LA, Robert J, Georas SN, Eliseeva S, Lawrence BP. Exposure to a mixture of 23 chemicals associated with unconventional oil and gas operations alters immune response to challenge in adult mice. J Immunotoxicol 2021; 18:105-117. [PMID: 34455897 DOI: 10.1080/1547691x.2021.1965677] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/20/2022] Open
Abstract
The prevalence of unconventional oil and gas (UOG) operations raises concerns regarding the potential for adverse health outcomes following exposure to water tainted by mixtures of UOG associated chemicals. The potential effects that exposure to complex chemical mixtures has on the immune system have yet to be fully evaluated. In this study, effects on the immune system of adult mice exposed to a mixture of 23 chemicals that have been associated with water near active UOG operations were investigated. Female and male mice were exposed to the mixture via their drinking water for at least 8 weeks. At the end of the exposure, cellularity of primary and secondary immune organs, as well as an immune system function, were assessed using three different models of disease, i.e. house dust mite (HDM)-induced allergic airway disease, influenza A virus infection, and experimental autoimmune encephalomyelitis (EAE). The results indicated exposures resulted in different impacts on T-cell populations in each disease model. Furthermore, the consequences of exposure differed between female and male mice. Notably, exposure to the chemical mixture significantly increased EAE disease severity in females, but not in male, mice. These findings indicated that direct exposure to this mixture leads to multiple alterations in T-cell subsets and that these alterations differ between sexes. This suggested to us that direct exposure to UOG-associated chemicals may alter the adult immune system, leading to dysregulation in immune cellularity and function.
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Affiliation(s)
- Colleen T O'Dell
- Department of Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
| | - Lisbeth A Boule
- Department of Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
| | - Jacques Robert
- Department of Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.,Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
| | - Steve N Georas
- Department of Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.,Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
| | - Sophia Eliseeva
- Department of Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
| | - B Paige Lawrence
- Department of Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.,Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
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11
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Di Ciaula A, Portincasa P. Relationships between emissions of toxic airborne molecules and type 1 diabetes incidence in children: An ecologic study. World J Diabetes 2021; 12:673-684. [PMID: 33995854 PMCID: PMC8107975 DOI: 10.4239/wjd.v12.i5.673] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/18/2020] [Revised: 03/17/2021] [Accepted: 04/12/2021] [Indexed: 02/06/2023] Open
Abstract
BACKGROUND Type 1 diabetes originates from gene-environment interactions, with increasing incidence over time.
AIM To identify correlates of childhood type 1 diabetes in European countries using an ecological approach. Several environmental variables potentially influencing the onset of type 1 diabetes have been previously evaluated. However, the relationships between epidemiologic data and exposure to toxic airborne molecules are scarcely studied.
METHODS We employed an ecological model to explore, in a wide time period (1990-2018), associations between type 1 diabetes incidence in 19 European countries (systematic literature review) and the nationwide production of five widely diffused air pollutants: particulate matter < 10 μm (PM10), nitrogen oxides (NO), non-methane volatile organic compounds (VOCs), sulphur oxide (SO2), and ammonia.
RESULTS Data confirm a raising incidence of type 1 diabetes in 18 out of 19 explored countries. The average difference (last vs first report, all countries) was +6.9 × 100000/year, with values ranging from -1.4 (Germany) to +16.6 (Sweden) per 100000/year. Although the overall production of pollutants decreased progressively from 1990 to 2018, type 1 diabetes incidence was positively associated with the nationwide emissions of PM10, VOCs, and NO but not with those of SO2 and ammonia. Type 1 diabetes incidence was significantly higher in countries with high emissions than in those with low emissions of PM10 (27.5 ± 2.4 vs 14.6 ± 2.4 × 100000 residents, respectively), VOCs (24.5 ± 4.4 vs 13.2 ± 1.7 × 100000 residents, respectively), and NO (26.6 ± 3 vs 13.4 ± 2.4 × 100000 residents, respectively), but not of SO2 or ammonia.
CONCLUSION Evidence justify further studies to explore better links between long-term air quality and type 1 diabetes onset at the individual level, which should include exposures during pregnancy. In this respect, type 1 diabetes could be, at least in part, a preventable condition. Thus, primary prevention policies acting through a marked abatement of pollutant emissions might attenuate future type 1 diabetes incidence throughout Europe.
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Affiliation(s)
- Agostino Di Ciaula
- Clinica Medica "A. Murri", Department of Biomedical Sciences and Human Oncology, University of Bari Medical School, Bari 70124, Italy
- International Society of Doctors for Environment (ISDE), Via XXV Aprile n.34 – 52100 Arezzo, Italy
| | - Piero Portincasa
- Clinica Medica "A. Murri", Department of Biomedical Sciences and Human Oncology, University of Bari Medical School, Bari 70124, Italy
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12
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Xylene delays the development of Leydig cells in pubertal rats by inducing reactive oxidative species. Toxicology 2021; 454:152740. [PMID: 33662507 DOI: 10.1016/j.tox.2021.152740] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/29/2020] [Revised: 02/07/2021] [Accepted: 02/25/2021] [Indexed: 11/23/2022]
Abstract
Xylene is a cyclic hydrocarbon, which is commonly used as a solvent in dyes, paints, polishes, and industrial solutions. It is a potential environmental pollutant. Here, we report the effect of xylene exposure on Leydig cell development in male rats during puberty. Xylene (0, 150, 750, and 1500 mg/kg) was gavaged to 35-day-old male Sprague Dawley rats for 21 days. Xylene significantly reduced serum testosterone levels at 750 and 1500 mg/kg without affecting serum luteinizing hormone and follicle-stimulating hormone levels. Xylene reduced the number of HSD11B1-positive Leydig cells at the advanced stage at 1500 mg/kg. At 750 and 1500 mg/kg, xylene also reduced the cell size and cytoplasm size. It down-regulated the expression of Leydig cell-specific genes (Lhcgr, Scarb1, Star, Cyp11a1, Hsd3b1, Cyp17a1, and Hsd11b1) and proteins. In addition, xylene significantly reduced the ratio of phosphorus-GSK-3β (pGSK-3β/GSK-3β), phosphorus-ERK1/2 (pERK)/ERK1/2, and phosphorus-AKT1 (pAKT1)/AKT1, and SIRT1 levels in the testes. In vitro Leydig cell culture showed that xylene induced oxidative stress by increasing the production of reactive oxygen species and lowing antioxidant (Sod2), and inhibited the production of testosterone, and down-regulated the expression of genes related to steroidogenesis, while vitamin E reversed the xylene-mediated effect as an antioxidant. In conclusion, xylene exposure may disrupt the development of pubertal Leydig cells by increasing reactive oxygen species production and reducing the expression of GSK-3β, ERK1/2, AKT1, and SIRT1.
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13
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Cakmak S, Cole C, Hebbern C, Andrade J, Dales R. Associations between blood volatile organic compounds, and changes in hematologic and biochemical profiles, in a population-based study. ENVIRONMENT INTERNATIONAL 2020; 145:106121. [PMID: 32947160 DOI: 10.1016/j.envint.2020.106121] [Citation(s) in RCA: 33] [Impact Index Per Article: 6.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/17/2020] [Revised: 09/03/2020] [Accepted: 09/04/2020] [Indexed: 06/11/2023]
Abstract
OBJECTIVE To investigate the influence of volatile organic compound (VOC) levels in blood, on hematological and serum biochemical parameters in the Canadian population. METHODS We tested the association between seven selected VOCs and hematological profiles and serum tests reflecting liver and kidney function and glucose metabolism using a cross-sectional study design in 3950 participants of the Canadian Health Measures Survey from 2012 to 2015. We used generalized linear mixed models adjusting for age, sex, smoking, alcohol consumption, BMI, education and household income. RESULTS An increase in blood concentration equivalent to the geometric mean for benzene, ethylbenzene, toluene, m-, p-xylenes, styrene, and total xylenes was associated with 0.68% (95% CI 0.36, 1.0) to 0.91% (95% CI 0.52, 1.3) increase in hemoglobin, and a 1.79% (95% CI 0.96, 2.62) to 4.11% (95% CI 3.11, 5.11) increase in total white blood cell count. Ethylbenzene, toluene, m-, p-xylenes and styrene were positively associated with increased platelet counts. A geometric mean increase for all VOCs was associated with decreases in creatinine. m- and p-xylenes were associated with a significant change in every measured blood cell count and liver function parameter, and in creatinine. Ethylbenzene was also positively associated with an increase in every measured hematologic parameter, two of the three liver function tests, and creatinine. Results were similar when stratified by age, but differed by smoking status and sex. CONCLUSIONS This study provides evidence that VOCs in blood, at levels found in the Canadian population, may influence blood cell counts and indicators of liver and kidney function, including an inverse association between serum VOC and creatinine. This novel finding merits further investigation to understand the impact of VOCs on human physiology and population health.
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Affiliation(s)
- Sabit Cakmak
- Population Studies Division, Environmental Health Science & Research Bureau, Health Canada, 101 Tunney's Pasture Driveway, Ottawa, ON K1A 0K9, Canada.
| | - Christie Cole
- Indoor Air Contaminants Assessment Section, Healthy Environments and Consumer Safety Branch, Health Canada, 269 Laurier Avenue, Ottawa, ON K1A 0K9, Canada
| | - Chris Hebbern
- Climate Change and Innovation Bureau, Health Canada, 269 Laurier Avenue, Ottawa, ON K1A 0K9, Canada
| | - Julie Andrade
- Population Studies Division, Environmental Health Science & Research Bureau, Health Canada, 101 Tunney's Pasture Driveway, Ottawa, ON K1A 0K9, Canada
| | - Robert Dales
- Population Studies Division, Environmental Health Science & Research Bureau, Health Canada and Ottawa Hospital Research Institute. 101 Tunney's Pasture Driveway, Ottawa, ON K1A 0K9, Canada; University of Ottawa and Ottawa Hospital Research Institute, Canada
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14
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Li ZY, Lu J, Zhang NZ, Chen J, Zhu XQ. Immune Responses Induced by HSP60 DNA Vaccine against Toxoplasma gondii Infection in Kunming Mice. THE KOREAN JOURNAL OF PARASITOLOGY 2018; 56:237-245. [PMID: 29996627 PMCID: PMC6046561 DOI: 10.3347/kjp.2018.56.3.237] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 02/09/2018] [Accepted: 04/23/2018] [Indexed: 12/31/2022]
Abstract
Toxoplasma gondii can infect all the vertebrates including human, and leads to serious toxoplasmosis and considerable veterinary problems. T. gondii heat shock protein 60 (HSP60) is associated with the activation of antigen presenting cells by inducing initial immune responses and releasing inflammatory cytokines. It might be a potential DNA vaccine candidate for this parasite. A pVAX-HSP60 DNA vaccine was constructed and immune responses was evaluated in Kunming mice in this study. Our data indicated that the innate and adaptive immune responses was elicited by successive immunizations with pVAX-HSP60 DNA, showing apparent increases of CD3e+CD4+ and CD3e+CD8a+ T cells in spleen tissues of the HSP60 DNA-immunized mice (24.70±1.23% and 10.90±0.89%, P<0.05) and higher levels of specific antibodies in sera. Furthermore, the survival period of the immunized mice (10.53±4.78 day) were significantly prolonged during the acute T. gondii infection. Decrease of brain cysts was significant in the experimental group during the chronic infection (P<0.01). Taken together, TgHSP60 DNA can be as a vaccine candidate to prevent the acute and chronic T. gondii infections.
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Affiliation(s)
- Zhong-Yuan Li
- College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province 230036, P. R. China.,State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, P. R. China
| | - Jing Lu
- Guangdong Wens Dahuanong Biotechnology Co., Ltd, Yunfu, Guangdong Province 524700, P. R. China
| | - Nian-Zhang Zhang
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, P. R. China
| | - Jia Chen
- Ningbo University School of Medicine, Ningbo, Zhejiang Province 315211, P. R. China
| | - Xing-Quan Zhu
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, P. R. China.,Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu Province 225009, P. R. China
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15
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Boulé LA, Chapman TJ, Hillman SE, Kassotis CD, O’Dell C, Robert J, Georas SN, Nagel SC, Lawrence BP. Developmental Exposure to a Mixture of 23 Chemicals Associated With Unconventional Oil and Gas Operations Alters the Immune System of Mice. Toxicol Sci 2018; 163:639-654. [PMID: 29718478 PMCID: PMC5974794 DOI: 10.1093/toxsci/kfy066] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/11/2023] Open
Abstract
Chemicals used in unconventional oil and gas (UOG) operations have the potential to cause adverse biological effects, but this has not been thoroughly evaluated. A notable knowledge gap is their impact on development and function of the immune system. Herein, we report an investigation of whether developmental exposure to a mixture of chemicals associated with UOG operations affects the development and function of the immune system. We used a previously characterized mixture of 23 chemicals associated with UOG, and which was demonstrated to affect reproductive and developmental endpoints in mice. C57Bl/6 mice were maintained throughout pregnancy and during lactation on water containing two concentrations of this 23-chemical mixture, and the immune system of male and female adult offspring was assessed. We comprehensively examined the cellularity of primary and secondary immune organs, and used three different disease models to probe potential immune effects: house dust mite-induced allergic airway disease, influenza A virus infection, and experimental autoimmune encephalomyelitis (EAE). In all three disease models, developmental exposure altered frequencies of certain T cell sub-populations in female, but not male, offspring. Additionally, in the EAE model disease onset occurred earlier and was more severe in females. Our findings indicate that developmental exposure to this mixture had persistent immunological effects that differed by sex, and exacerbated responses in an experimental model of autoimmune encephalitis. These observations suggest that developmental exposure to complex mixtures of water contaminants, such as those derived from UOG operations, could contribute to immune dysregulation and disease later in life.
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Affiliation(s)
| | - Timothy J Chapman
- Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14842
| | - Sara E Hillman
- Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14842
| | - Christopher D Kassotis
- Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14842
- Department of Obstetrics, Gynecology and Women’s Health, School of Medicine, University of Missouri, Columbia, MO 65212
| | | | - Jacques Robert
- Department of Environmental Medicine
- Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York
| | - Steve N Georas
- Department of Environmental Medicine
- Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14842
- Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York
| | - Susan C Nagel
- Department of Obstetrics, Gynecology and Women’s Health, School of Medicine, University of Missouri, Columbia, MO 65212
| | - B Paige Lawrence
- Department of Environmental Medicine
- Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York
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16
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Mediating Role of TRPV1 Ion Channels in the Co-exposure to PM2.5 and Formaldehyde of Balb/c Mice Asthma Model. Sci Rep 2017; 7:11926. [PMID: 28931832 PMCID: PMC5607312 DOI: 10.1038/s41598-017-11833-6] [Citation(s) in RCA: 24] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/05/2017] [Accepted: 07/24/2017] [Indexed: 01/31/2023] Open
Abstract
Asthma is a complex pulmonary inflammatory disease that can be promoted by air pollutants such as PM2.5 and formaldehyde (FA). However, existent experimental evidence principally focuses on the negative influence of a single air pollutant, neglecting the possible synergistic effect in biological responses to mixture of these pollutants, a more common situation in our daily life. In this study, allergic Balb/c mice were exposed to a mixture of PM2.5 and FA, and their toxicological effects and mechanisms were explored. It is demonstrated that the combined exposure to PM2.5 and FA can greatly aggravate allergic asthma in mice. When compared with exposure to PM2.5 or FA alone, the co-exposure showed a certain synergistic effect. Increased levels of ROS, inflammatory factors and total serum immunoglobulin E were concomitant with this deterioration. Furthermore, results suggested that co-exposure exacerbated the activation of TRPV1 signal pathways, with an enhancement in substance P and calcitonin gene-related peptide production, which contributed to inflammation in asthma by neurogenic inflammation. The study also proved that capsazepine treatment could reduce the levels of not only pro-inflammatory neuropeptides, but also oxidative stress. It is concluded that co-exposure to PM2.5 and FA exacerbated allergic asthma through oxidative stress and enhanced TRPV1 activation.
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17
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Wen H, Yuan L, Wei C, Zhao Y, Qian Y, Ma P, Ding S, Yang X, Wang X. Effects of combined exposure to formaldehyde and benzene on immune cells in the blood and spleen in Balb/c mice. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 2016; 45:265-273. [PMID: 27343751 DOI: 10.1016/j.etap.2016.05.007] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/17/2015] [Revised: 05/05/2016] [Accepted: 05/07/2016] [Indexed: 06/06/2023]
Abstract
Formaldehyde and benzene are the two major indoor air pollutants due to their prevalence and toxicity. This study aimed to explore the toxic effect on the spleen and relevant immune responses of Balb/c mice caused by exposure to a combination of formaldehyde and benzene. Balb/c mice were divided randomly into five groups (n=9/group): blank control group (Ctrl); solvent ([corn] Oil) control; formaldehyde only (FA, 3mg/m(3)); benzene only (BZ, 150mg/kg BW); and, formaldehyde+benzene group (FA+BZ). Exposures were performed for 8h/day, 5 day/week, for 2 weeks. Tail blood was collected after the final exposure; 24-h later, the mice were euthanized to permit assessment of a variety of immune endpoints. The endpoints' three areas were: (1) in living mice, body weight and delayed-type hypersensitivity (DTH) responses; (2) in blood, immune cell counts and serum antibody levels (serum hemagglutination); and, (3) in spleen samples, reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), caspase-3 (cell apoptosis) levels and lymphocyte proliferation. In this study we fund (1) BZ and FA+BZ exposure can lead to the reduction in the number of some immune cells in peripheral blood; (2) Formaldehyde has certain synergistic effects on benzene-induced cytotoxicity in peripheral blood, (3) FA, BZ and FA+BZ exposure can lead to ROS and GSH depletion in spleen cells, and spleen cell apoptosis (caspase-3 increased) may be one of the downstream events, decreased splenic lymphocyte proliferation; and (4) the FA+BZ combined exposure can lead to the decreased body weight, serum antibody level (by serum hemagglutination assay).
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Affiliation(s)
- Huaxiao Wen
- Laboratory of Environmental Biomedicine, School of Life Science, Central China Normal University, Wuhan, China
| | - Langyue Yuan
- Laboratory of Environmental Biomedicine, School of Life Science, Central China Normal University, Wuhan, China
| | - Chenxi Wei
- Laboratory of Environmental Biomedicine, School of Life Science, Central China Normal University, Wuhan, China
| | - Yun Zhao
- Laboratory of Environmental Biomedicine, School of Life Science, Central China Normal University, Wuhan, China
| | - Yan Qian
- State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, China
| | - Ping Ma
- College of Basic Medical Sciences, Hubei University of Science and Technology, Xianning, China
| | - Shumao Ding
- Laboratory of Environmental Biomedicine, School of Life Science, Central China Normal University, Wuhan, China
| | - Xu Yang
- Laboratory of Environmental Biomedicine, School of Life Science, Central China Normal University, Wuhan, China; College of Basic Medical Sciences, Hubei University of Science and Technology, Xianning, China.
| | - Xianliang Wang
- Institute of Environmental Health and Related Product Safety, Chinese Centre for Disease Control and Prevention, Beijing, China.
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