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Emami Aref P, Khoshdel A, Nicknia S, Mahmoodi M, Hajizadeh MR, Mirzaiey MR, Fahmidehkar MA. Effect of Hydroalcoholic Extract of Chamomile, Aloe Vera, and Green Tea on the Diabetic Wound in Rats. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, INDIA SECTION B: BIOLOGICAL SCIENCES 2024; 94:803-810. [DOI: 10.1007/s40011-024-01594-4] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/18/2022] [Revised: 11/11/2022] [Accepted: 03/28/2024] [Indexed: 01/04/2025]
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Saifi A, Sharma A, Chaudhary A, Siddiqui N, Ashwlayan VD, Singh B. Unveiling the Latest Breakthroughs: A Comprehensive Review of the Therapeutic Activity and Safety Profile of Aloe vera. Curr Drug Saf 2024; 19:407-416. [PMID: 38204271 DOI: 10.2174/0115748863274759231221093309] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/25/2023] [Revised: 10/25/2023] [Accepted: 10/27/2023] [Indexed: 01/12/2024]
Abstract
The use of herbal drugs as alternative and complementary medicine has increased in popularity, raising concerns about their safety profile. Aloe vera, a plant with diverse therapeutic properties, has been extensively used for centuries. This review aims to assess the therapeutic activity and safety profile of Aloe vera. A comprehensive literature search was conducted to gather relevant information from various biomedical databases. The chemical composition, mechanism of action, and therapeutic activities of Aloe vera were analyzed. Aloe vera contains numerous active components such as vitamins, enzymes, minerals, sugars, lignin, saponins, and anthraquinones. Its mechanisms of action involve collagen synthesis, anti-inflammatory effects, immune modulation, laxative properties, and antiviral activity. Aloe vera has demonstrated potential therapeutic benefits in wound healing, diabetes management, liver and kidney protection, and glycemic control. However, it is essential to consider potential side effects, such as skin irritation and allergic reactions. This review provides evidence-based information to improve patient safety and promote informed decisions regarding the use of Aloe vera as a therapeutic agent.
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Affiliation(s)
- Alimuddin Saifi
- Department of Pharmaseutical, Meerut Institute of Engineering and Technology, Meerut-250005, Uttar Pradesh, India
| | - Alok Sharma
- Department of Pharmaseutical, Meerut Institute of Engineering and Technology, Meerut-250005, Uttar Pradesh, India
| | - Anurag Chaudhary
- Department of Pharmaseutical, Meerut Institute of Engineering and Technology, Meerut-250005, Uttar Pradesh, India
| | - Nazia Siddiqui
- Department of Pharmaseutical, Meerut Institute of Engineering and Technology, Meerut-250005, Uttar Pradesh, India
| | - Vrish Dhwaj Ashwlayan
- Department of Pharmaseutical, Meerut Institute of Engineering and Technology, Meerut-250005, Uttar Pradesh, India
| | - Bhuwanendra Singh
- Department of Pharmacognosy, SD College of Pharmacy & Vocational Studies, Muzaffarnagar UP, India
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Sultana A, Borgohain R, Rayaji A, Saha D, Kumar Das B. Promising Phytoconstituents in Diabetes-related Wounds: Mechanistic Insights and Implications. Curr Diabetes Rev 2024; 21:e270224227477. [PMID: 38424430 DOI: 10.2174/0115733998279112240129074457] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 09/11/2023] [Revised: 01/05/2024] [Accepted: 01/16/2024] [Indexed: 03/02/2024]
Abstract
BACKGROUND The onset of diabetes mellitus (DM), a metabolic disorder characterized by high blood glucose levels and disrupted glucose metabolism, results in 20% of people with diabetes suffering from diabetes-related wounds worldwide. A minor wound, such as a cut or abrasion, can lead to infections and complications in diabetic patients. We must understand the mechanism/s contributing to this delayed wound healing to develop effective prevention strategies. The potential benefits of bioactive phytochemicals for diabetic wound healing have been reported in numerous studies. METHOD A bioactive compound may have multiple actions, including antioxidants, antiinflammatory, antimicrobial, and angiogenesis. Compounds derived from these plants have shown promising results in wound healing, inflammation reduction, collagen synthesis, and neovascularization improvement. RESULTS Consequently, this review provides an update to our understanding of how phytoconstituents promote wound healing in diabetics. A thorough literature review was conducted on diabetes, wound healing, and phytoconstituents for this study. Only English publications until June 2023 were included in the search, which used multiple search engines and the main keywords. Summing up, phytochemical-based interventions might improve the quality of life for diabetics by improving wound healing. CONCLUSION However, to fully understand the efficacy and safety of these phytochemicals in managing diabetic wounds, more research and clinical trials are needed.
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Affiliation(s)
- Arjina Sultana
- Department of Pharmacology, School of Pharmaceutical Sciences, Girijananda Chowdhury University, Azara, Guwahati 781017, Assam, India
| | - Ranadeep Borgohain
- Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Girijananda Chowdhury University, Azara, Guwahati 781017, Assam, India
| | - Ashwini Rayaji
- Department of Pharmaceutical Chemistry, KRE's Karnataka College of Pharmacy, Bidar 585403, Karnataka, India
| | - Dipankar Saha
- Department of Pharmacology, School of Pharmaceutical Sciences, Girijananda Chowdhury University, Azara, Guwahati 781017, Assam, India
| | - Bhrigu Kumar Das
- Department of Pharmacology, School of Pharmaceutical Sciences, Girijananda Chowdhury University, Azara, Guwahati 781017, Assam, India
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Atajanova T, Rahman MM, Konieczkowski DJ, Morris ZS. Radiation-associated secondary malignancies: a novel opportunity for applying immunotherapies. Cancer Immunol Immunother 2023; 72:3445-3452. [PMID: 37658906 PMCID: PMC10992240 DOI: 10.1007/s00262-023-03532-1] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/17/2023] [Accepted: 08/16/2023] [Indexed: 09/05/2023]
Abstract
Radiation is commonly used as a treatment intended to cure or palliate cancer patients. Despite remarkable advances in the precision of radiotherapy delivery, even the most advanced forms inevitably expose some healthy tissues surrounding the target site to radiation. On rare occasions, this results in the development of radiation-associated secondary malignancies (RASM). RASM are typically high-grade and carry a poorer prognosis than their non-radiated counterparts. RASM are characterized by a high mutation burden, increased T cell infiltration, and a microenvironment that bears unique inflammatory signatures of prior radiation, including increased expression of various cytokines (e.g., TGF-β, TNF-α, IL4, and IL10). Interestingly, these cytokines have been shown to up-regulate the expression of PD-1 and/or PD-L1-an immune checkpoint receptor/ligand pair that is commonly targeted by immune checkpoint blocking immunotherapies. Here, we review the current understanding of the tumor-immune interactions in RASM, highlight the distinct clinical and molecular characteristics of RASM that may render them immunologically "hot," and propose a rationale for the formal testing of immune checkpoint blockade as a treatment approach for patients with RASM.
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Affiliation(s)
- Tavus Atajanova
- Biochemistry and Biophysics Program, Amherst College, Amherst, MA, 01002, USA
- Department of Sociology, Amherst College, Amherst, MA, 01002, USA
- Department of Human Oncology, University of Wisconsin-Madison, Madison, WI, 53726, USA
| | - Md Mahfuzur Rahman
- Department of Human Oncology, University of Wisconsin-Madison, Madison, WI, 53726, USA
| | - David J Konieczkowski
- Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, Columbus, OH, 43210, USA
| | - Zachary S Morris
- Department of Human Oncology, University of Wisconsin-Madison, Madison, WI, 53726, USA.
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Bai Y, Niu Y, Qin S, Ma G. A New Biomaterial Derived from Aloe vera-Acemannan from Basic Studies to Clinical Application. Pharmaceutics 2023; 15:1913. [PMID: 37514099 PMCID: PMC10385217 DOI: 10.3390/pharmaceutics15071913] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/07/2023] [Revised: 06/26/2023] [Accepted: 07/04/2023] [Indexed: 07/30/2023] Open
Abstract
Aloe vera is a kind of herb rich in polysaccharides. Acemannan (AC) is considered to be a natural polysaccharide with good biodegradability and biocompatibility extracted from Aloe vera and has a wide range of applications in the biomedical field due to excellent immunomodulatory, antiviral, antitumor, and tissue regeneration effects. In recent years, clinical case reports on the application of AC as a novel biomedical material in tissue regenerative medicine have emerged; it is mainly used in bone tissue engineering, pulp-dentin complex regeneration engineering, and soft tissue repair, among other operations. In addition, multiple studies have proved that the new composite products formed by the combination of AC and other compounds have excellent biological and physical properties and have broader research prospects. This paper introduces the preparation process, surface structure, and application forms of AC; summarizes the influence of acetyl functional group content in AC on its functions; and provides a detailed review of the functional properties, laboratory studies, clinical cutting-edge applications, and combined applications of AC. Finally, the current application status of AC from basic research to clinical treatment is analyzed and its prospects are discussed.
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Affiliation(s)
- Yingjie Bai
- School of Stomatology, Dalian Medical University, No. 9 West Section, Lvshunnan Road, Dalian 116044, China
- Academician Laboratory of Immune and Oral Development & Regeneration, Dalian Medical University, Lvshun South Road, Dalian 116044, China
| | - Yimeng Niu
- School of Stomatology, Dalian Medical University, No. 9 West Section, Lvshunnan Road, Dalian 116044, China
- Academician Laboratory of Immune and Oral Development & Regeneration, Dalian Medical University, Lvshun South Road, Dalian 116044, China
| | - Shengao Qin
- School of Stomatology, Dalian Medical University, No. 9 West Section, Lvshunnan Road, Dalian 116044, China
- Academician Laboratory of Immune and Oral Development & Regeneration, Dalian Medical University, Lvshun South Road, Dalian 116044, China
| | - Guowu Ma
- School of Stomatology, Dalian Medical University, No. 9 West Section, Lvshunnan Road, Dalian 116044, China
- Academician Laboratory of Immune and Oral Development & Regeneration, Dalian Medical University, Lvshun South Road, Dalian 116044, China
- Department of Stomatology, Stomatological Hospital Affiliated School, Stomatology of Dalian Medical University, NO. 397 Huangpu Road, Shahekou District, Dalian 116086, China
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Iqbal DN, Munir A, Abbas M, Nazir A, Ali Z, Alshawwa SZ, Iqbal M, Ahmad N. Polymeric Membranes of Chitosan/Aloe Vera Gel Fabrication With Enhanced Swelling and Antimicrobial Properties for Biomedical Applications. Dose Response 2023; 21:15593258231169387. [PMID: 37056472 PMCID: PMC10087668 DOI: 10.1177/15593258231169387] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/10/2022] [Accepted: 03/27/2023] [Indexed: 04/15/2023] Open
Abstract
Since ancient times, medicinal plants have been used as traditional medicine to treat a variety of ailments. Aloe vera (AV) gel's therapeutic potential is one of the most effective approach in the fabrication of functional materials. The current study aimed to prepare the AV and chitosan (CS) membranes using various cross-linkers that were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform-infrared (FT-IR) spectroscopy, thermal gravimetric analysis (TGA), and ultraviolet-visible (UV-Visible) techniques, as well as swelling ratio and antimicrobial studies. SEM analysis revealed that the membrane is porous, with interconnected pores. The inclusion of AV contents in the membrane improved thermal stability and crystallinity. The swelling ratio of the ACPG-3 membrane with a 2:1 CS to AV ratio was 366%. The membranes showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pasteurella multocida strains. The findings revealed that polymeric CS/AV membranes have effective potential for use in the biomedical field.
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Affiliation(s)
- Dure N. Iqbal
- Department of Chemistry, The University of
Lahore, Lahore, Pakistan
| | - Atira Munir
- Department of Chemistry, The University of
Lahore, Lahore, Pakistan
| | - Mazhar Abbas
- Department of Biochemistry, University of Veterinary and Animal
Sciences, Lahore, Pakistan
| | - Arif Nazir
- Department of Chemistry, The University of
Lahore, Lahore, Pakistan
- Arif Nazir, Department of Chemistry, The
University of Lahore, Lahore 53700, Pakistan.
| | - Zahid Ali
- Department of Chemistry, The University of
Lahore, Lahore, Pakistan
- State Key-Laboratory of Organic
Inorganic-Composites, Beijing University of Chemical
Technology, Beijing, China
| | - Samar Z. Alshawwa
- Department of Pharmaceutical
Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman
University, Riyadh, Saudi Arabia
| | - Munawar Iqbal
- Department of Chemistry, The University of
Lahore, Lahore, Pakistan
- Department of Chemistry, Division
of Science and Technology, University of Education, Lahore, Pakistan
| | - Naveed Ahmad
- Department of Chemistry, Division
of Science and Technology, University of Education, Lahore, Pakistan
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Rayate AS, Nagoba BS, Mumbre SS, Mavani HB, Gavkare AM, Deshpande AS. Current scenario of traditional medicines in management of diabetic foot ulcers: A review. World J Diabetes 2023; 14:1-16. [PMID: 36684382 PMCID: PMC9850800 DOI: 10.4239/wjd.v14.i1.1] [Citation(s) in RCA: 4] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/24/2022] [Revised: 11/04/2022] [Accepted: 12/06/2022] [Indexed: 01/10/2023] Open
Abstract
Diabetic foot infections and diabetic foot ulcers (DFU) cause significant suffering and are often recurring. DFU have three important pathogenic factors, namely, microangiopathy causing local tissue anoxia, neuropathy making the foot prone to injuries from trivial trauma, and local tissue hyperglycaemia favouring infection and delaying the wound healing. DFU have been the leading cause for non-traumatic amputations of part or whole of the limb. Western medicines focus mainly on euglycaemia, antimicrobials, debridement and wound cover with grafts, and off-loading techniques. Advances in euglycaemic control, foot care and footwear, systemic antimicrobial therapy, and overall health care access and delivery, have resulted in an overall decrease in amputations. However, the process of wound care after adequate debridement remains a major cost burden globally, especially in developing nations. This process revolves around two basic concerns regarding control/eradication of local infection and promotion of faster healing in a chronic DFU without recurrence. Wound modulation with various dressings and techniques are often a costly affair. Some aspects of the topical therapy with modern/Western medicines are frequently not addressed. Cost of and compliance to these therapies are important as both the wounds and their treatment are “chronic.” Naturally occurring agents/medications from traditional medicine systems have been used frequently in different cultures and nations, though without adequate clinical base/relevance. Traditional Chinese medicine involves restoring yin-yang balance, regulating the ‘chi’, and promoting local blood circulation. Traditional medicines from India have been emphasizing on ‘naturally’ available products to control wound infection and promote all the aspects of wound healing. There is one more group of chemicals which are not pharmaceutical agents but can create acidic milieu in the wound to satisfy the above-mentioned basic concerns. Various natural and plant derived products (e.g., honey, aloe vera, oils, and calendula) and maggots are also used for wound healing purposes. We believe that patients with a chronic wound are so tired physically, emotionally, and financially that they usually accept native traditional medicine which has the same cultural base, belief, and faith. Many of these products have never been tested in accordance to “evidence-based medicine.” There are usually case reports and experience-based reports about these products. Recently, there have been some trials (in vitro and in vivo) to verify the claims of usage of traditional medicines in management of DFU. Such studies show that these natural products enhance the healing process by controlling infection, stimulating granulation tissue, antimicrobial action, promoting fibroblastic activity and collagen deposition, etc. In this review, we attempt to study and analyse the available literature on results of topical traditional medicines, which are usually advocated in the management of DFU. An integrated and ‘holistic’ approach of both modern and traditional medicine may be more acceptable to the patient, cost effective, and easy to administer and monitor. This may also nevertheless lead to further improvement in quality of life and decrease in the rates of amputations for DFU.
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Affiliation(s)
- Abhijit S Rayate
- Department of Surgery, Maharashtra Institute of Medical Sciences & Research (Medical College), Latur 413531, India
| | - Basavraj S Nagoba
- Department of Microbiology, Maharashtra Institute of Medical Sciences & Research (Medical College), Latur 413531, India
| | - Sachin S Mumbre
- Department of Community Medicine, Ashwini Rural Medical College, Solapur 413006, India
| | - Hardi B Mavani
- Department of Surgery, Maharashtra Institute of Medical Sciences & Research (Medical College), Latur 413531, India
| | - Ajay M Gavkare
- Department of Physiology, Maharashtra Institute of Medical Sciences & Research (Medical College), Latur 413531, India
| | - Advait S Deshpande
- Department of Surgery, Maharashtra Institute of Medical Sciences & Research (Medical College), Latur 413531, India
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Zakian A, Ahmadi HA, Keleshteri MH, Madani A, Tehrani-Sharif M, Rezaie A, Davoodi F, Kish GF, Raisi A, Langerudi MT, Pasha MBM. Study on the effect of medicinal leech therapy (Hirudo medicinalis) on full-thickness excisional wound healing in the animal model. Res Vet Sci 2022; 153:153-168. [PMID: 36395588 DOI: 10.1016/j.rvsc.2022.10.015] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/09/2019] [Revised: 08/28/2022] [Accepted: 10/20/2022] [Indexed: 11/15/2022]
Abstract
The possible impacts of alternative and conventional medicines on wound healing are now of growing interest. This study aimed to evaluate and elucidate the wound healing activity of medicinal leech therapy in wound excision of the rat model. After a round, full-thickness excision was made in the dorsal region of the body, the animals (n = 30) were randomly divided into three equal groups: I) the treatment group (MLT), where the wounds received leech treatment; II) the positive control group (PC), where the wounds received 1% sodium phenytoin treatment; and III) the negative control group (NC), where the wounds did not receive any treatment. On days 6 and 16, wound biopsy specimens were taken, and prepared sections were stained using various methods. The contraction rate differed significantly (P < 0.05) between the NC group and the other groups. The histopathological evaluation revealed that MLT group showed an accelerated healing process and lower inflammatory response compared to other groups. In ML-treated group maturation and remodeling of collagen had occurred, while in 1% sodium phenytoin treated group, proliferation was the prominent feature. Results showed that the fibroblast was significantly lower in the NC group in comparison to other groups. The number of MNC, s, and PMN, s was significantly higher in the NC group compared to other groups (P < 0.0001). In our study, medicinal leech therapy had a higher success rate in healing for the treatment of excisional wounds in animal models.
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Affiliation(s)
- Amir Zakian
- Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.
| | - Hamid Amir Ahmadi
- DVM, Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Semnan, Iran
| | | | - Amir Madani
- DVM, Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Semnan, Iran
| | - Meysam Tehrani-Sharif
- Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Semnan, Iran
| | - Annahita Rezaie
- Department of Pathobiology, College of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran
| | - Farshid Davoodi
- Resident in Veterinary Surgery, Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
| | - Ghasem Farjani Kish
- Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
| | - Abbas Raisi
- Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
| | - Matin Taghipour Langerudi
- DVM, Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Shabestar Branch, Shabestar, Iran
| | - Milad Babaii Moghadam Pasha
- DVM, Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Babol Branch, Babol, Iran
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Hormesis: wound healing and fibroblasts. Pharmacol Res 2022; 184:106449. [PMID: 36113746 DOI: 10.1016/j.phrs.2022.106449] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/15/2022] [Revised: 09/09/2022] [Accepted: 09/10/2022] [Indexed: 11/23/2022]
Abstract
Hormetic dose responses are reported here to occur commonly in the dermal wound healing process, with the particular focus on cell viability, proliferation, migration and collagen deposition of human and murine fibroblasts with in vitro studies. Hormetic responses were induced by a wide range of substances, including endogenous agents, pharmaceutical preparations, plant-derived extracts including many well-known dietary supplements, as well as physical stressor agents such as low-level laser treatments. Detailed mechanistic studies have identified common signaling pathways and their cross-pathway communications that mediate the hormetic dose responses. These findings complement and extend a similar comprehensive assessment concerning the occurrence of hormetic dose responses in keratinocytes. These findings demonstrate the generality of the hormetic dose response for key wound healing endpoints, suggesting that the hormesis concept has a fundamental role in wound healing, with respect to guiding strategies for experimental evaluation as well as therapeutic applications.
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Gopal D, Ramani M, George R, Janakiraman N. Understanding the cellular response of human tenon fibroblast on polycaprolactone-Aloe vera blend fiber. J Biomater Appl 2022; 37:375-388. [PMID: 35446716 DOI: 10.1177/08853282221091042] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/15/2022]
Abstract
The signalling response is determined by the cell's reaction to different biochemical and biophysical inputs such as stiffness, topological, and structural alignment. The surface patterns at the nano-scale can be an influential factor in cell signalling behaviour. It is important to understand the cellular response to the biophysical cues for biomedical applications. Biomaterials have an important role in regenerative tissue engineering. In this study, we have fabricated electrospun polycaprolactone (PCL) and PCL-Aloe vera (PCL-AV) nanofibrous matrix and studied its effect on the human tenon fibroblast (HTF) cellular and morphological changes. The electrospun fibers were characterized using Scanning Electron Microscope (SEM), Fourier Transform Infrared spectroscopy (FTIR), Atomic Force Microscopy (AFM) and Brunaur, Emette and Teller (BET) analysis for their morphology, composition, topography, surface area and porosity. The results revealed fiber size, roughness and porosity has been altered by addition of AV. The HTF cell viability, proliferation and expression of focal adhesion proteins, such as FAK, Ezrin, Vasp and Cofilin on the PCL-AV fiber matrix were examined. The results showed a change in cellular morphology and a significant change in the cofilin phosphorylation on PCL-AV nanofiber. The influence of Aloe vera composition on the nano-dimension of the PCL has made a significant impact on the cellular morphology at both gene and protein levels. This observation suggests that AV composition in the nanofiber can significantly influence the HTF cellular adhesions.
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Affiliation(s)
- Divya Gopal
- 29853Vision Research Foundation, Chennai, Tamil Nadu, India
| | - Madhura Ramani
- 29853Vision Research Foundation, Chennai, Tamil Nadu, India
| | - Ronnie George
- Medical Research Foundation, 29853Sankara Nethralaya, Chennai, Tamil Nadu, India
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11
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Barrier effect and wound healing activity of the medical device REF-FTP78 in the treatment of gastroesophageal reflux disease. Sci Rep 2022; 12:6136. [PMID: 35414705 PMCID: PMC9005723 DOI: 10.1038/s41598-022-10171-6] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/08/2021] [Accepted: 04/04/2022] [Indexed: 12/16/2022] Open
Abstract
REF-FTP78 is a class IIb medical device present on the market with different trade names and developed for the treatment of gastroesophageal reflux disease (GERD). This medical device is based on polysaccharides from Aloe Barbadensis and fucoidans from brown seaweeds, such as Undaria pinnatifida and Fucus vesiculosus, and aims to exert a protective effect on the esophageal mucosa against the noxious components of refluxate. The present study reports on the efficacy of REF-FTP78 devoting a particular attention to the barrier effect and wound healing properties, combined with antioxidant and anti-inflammatory activities. Film-forming properties and barrier effect were investigated on in vitro reconstructed human esophageal epithelium, through TEER measurement and evaluation of caffeine and Lucifer yellow permeability, and in an ex vivo swine model of esophageal mucosa damage. Antioxidant and anti-inflammatory properties were evaluated in terms of scavenging activity towards DPPH, ABTS and NO radicals and a wound healing assay was carried out to study the influence of the product on cell migration. The obtained results highlighted a significant barrier effect, with a reduction in caffeine penetration equal to 65.3%, the ability to both repair and prevent the damage caused by an acid insult, confirmed by a good transepithelial resistance for the tissue treated with the tested item, and the capacity to promote wound healing. Furthermore, the tested product showed good antioxidant and anti-inflammatory properties in the performed radical scavenging assays. These findings support the use of REF-FTP78 in the treatment of GERD.
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Piaia L, Pittella CQP, Souza SSD, Berti FV, Porto LM. Incorporation of Aloe vera extract in bacterial nanocellulose membranes. POLIMEROS 2022. [DOI: 10.1590/0104-1428.210062] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Affiliation(s)
- Lya Piaia
- Universidade Federal de Santa Catarina, Brasil
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13
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Nzekwe IT, Agwuka OI, Okezie MU, Fasheun DO, Nnamani PO, Agubata CO. Designing an ideal alcohol-based hand sanitizer: in vitro antibacterial responses of ethanol and isopropyl alcohol solutions to changing composition. AAPS OPEN 2021; 7:5. [PMID: 34841045 PMCID: PMC8606244 DOI: 10.1186/s41120-021-00038-x] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/15/2021] [Accepted: 10/09/2021] [Indexed: 11/24/2022] Open
Abstract
This study aimed to achieve an in vitro quantification of the effects of composition and formulation factors on the killing rates of alcohol-based hand sanitizers. The killing rates of 85% ethyl alcohol (ET) and isopropyl alcohol (IPA) were studied under different conditions such as pH, electrolyte concentration, or inclusion of herbal extracts (cucumber, carrot, and aloe vera), a quaternary ammonium compound, or thickener over different time intervals. Changes in the activities were retested after 3 months as an indication of stability. From two-way ANOVA, both the time of exposure and the sanitizer type affected the activity against Staphylococcus aureus (P = 0.001 for both alcohols), whereas for Escherichia coli, time of exposure was significant (P = 0.027), while sanitizer type was less significant (P = 0.063). Extreme pHs, the presence of ions, and the inclusion of additives such as benzalkonium chloride (BAC), plant extracts, or carbomer impacted the 3-month activity of the samples differently. Important differences existing in the activities of ET and IPA, as a function of formulation factors or use conditions have been quantified using in vitro methods. Formulations should best be tailored for particular purposes and the all-purpose hand sanitizer may not exist. ![]()
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Affiliation(s)
- Ifeanyi T Nzekwe
- Department of Pharmaceutics & Pharmaceutical Technology, Nnamdi Azikiwe University, Awka, Anambra Nigeria
| | - Onyedika I Agwuka
- Department of Pharmaceutics & Pharmaceutical Technology, Nnamdi Azikiwe University, Awka, Anambra Nigeria
| | - Moses U Okezie
- Department of Pharmaceutical Microbiology & Biotechnology, Nnamdi Azikiwe University, Awka, Anambra Nigeria
| | - Daniel O Fasheun
- Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
| | - Petra O Nnamani
- Department of Pharmaceutics, University of Nigeria, Nsukka, Enugu Nigeria
| | - Chukwuma O Agubata
- Department of Pharmaceutical Technology and Industrial Pharmacy, University of Nigeria, Nsukka, Enugu State Nigeria
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Feasibility study for inducing the skeletal muscle fibrosis via irradiation using two mouse strains. Jpn J Radiol 2021; 40:466-475. [PMID: 34841459 DOI: 10.1007/s11604-021-01219-0] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/11/2021] [Accepted: 11/05/2021] [Indexed: 10/19/2022]
Abstract
PURPOSE Although the mechanism of onset and progression of radiation-induced fibrosis (RIF) has been studied, most studies to date have focused on pulmonary fibrosis. There are few studies on murine RIF in the skeletal muscle, and the pathogenic mechanism remains unclear. This pilot study aimed to evaluate the feasibility to create a murine model of RIF in the skeletal muscle and analyze strain differences in fibrosis sensitivity. MATERIALS AND METHODS Two mouse strains, C57BL/6 and C3H/He, were used. Their right hind limbs were irradiated at a dose of 25 Gy once a week for three fractions. Gastrocnemius muscles were collected at day 4, and weeks 2, 4, 8, 12, and 24 after the third irradiation and subjected to histopathological examination and immunoblotting. RESULTS In C57BL/6 mice, chronic inflammation and an increased expression of transforming growth factor-β (TGF-β) and fibronectin were observed 2 weeks after irradiation. A significant increase in fibrosis was detected after 8 weeks. However, in C3H/He mice, the expression of TGF-β and fibronectin increased 8 weeks after irradiation, and fibrosis significantly increased after 12 weeks. Moreover, the degrees of inflammation and fibrosis were more remarkable in C57BL/6 mice than in C3H/He mice. CONCLUSION The onset and degree of fibrosis may be associated with the expression of TGF-β and fibronectin, and inflammation, in a strain-specific manner. Therefore, a murine model of RIF in the skeletal muscle could be created using the indicated method, suggesting that the C57BL/6 strain is more sensitive to fibrosis in the skeletal muscle, as well as the lung, than the C3H/He strain. Radiation-induced fibrosis in the skeletal muscle could be detected in C57BL/6 and C3H/He mice, with C57BL/6 mice being more sensitive to fibrosis in the skeletal muscle than C3H/He mice.
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Sadgrove NJ, Simmonds MSJ. Pharmacodynamics of Aloe vera and acemannan in therapeutic applications for skin, digestion, and immunomodulation. Phytother Res 2021; 35:6572-6584. [PMID: 34427371 DOI: 10.1002/ptr.7242] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/27/2020] [Revised: 07/25/2021] [Accepted: 08/02/2021] [Indexed: 12/16/2022]
Abstract
Scientific studies of Aloe vera have tentatively explained therapeutic claims from a mechanistic perspective. Furthermore, in vitro outcomes demonstrate that the breakage of acemannan chains into smaller fragments enhances biological effects. These fragments can intravenously boost vaccine efficacy or entrain the immune system to attack cancer cells by mannose receptor agonism of macrophage or dendritic cells. With oral consumption, epithelialisation also occurs at injured sites in the small intestine or colon. The main advantage of dietary acemannan is the attenuation of the digestive process, increasing satiety, and slowing the release of sugars from starches. In the colon, acemannan is digested by microbes into short-chain fatty acids that are absorbed and augment the sensation of satiety and confer a host of other health benefits. In topical applications, an acemannan/chitosan combination accelerates the closure of wounds by promoting granular tissue formation, which creates a barrier between macrophages or neutrophils and the wound dressing. This causes M2 polarisation, reversal of inflammation, and acceleration of the re-epithelialisation process. This review summarises and explains the current pharmacodynamic paradigm in the context of acemannan in topical, oral, and intravenous applications. However, due to contradictory results in the literature, further research is required to provide scientific evidence to confirm or nullify these claims.
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Yazarlu O, Iranshahi M, Kashani HRK, Reshadat S, Habtemariam S, Iranshahy M, Hasanpour M. Perspective on the application of medicinal plants and natural products in wound healing: A mechanistic review. Pharmacol Res 2021; 174:105841. [PMID: 34419563 DOI: 10.1016/j.phrs.2021.105841] [Citation(s) in RCA: 80] [Impact Index Per Article: 20.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 06/08/2021] [Revised: 08/14/2021] [Accepted: 08/17/2021] [Indexed: 12/14/2022]
Abstract
Wound is defined as any injury to the body such as damage to the epidermis of the skin and disturbance to its normal anatomy and function. Since ancient times, the importance of wound healing has been recognized, and many efforts have been made to develop novel wound dressings made of the best material for rapid and effective wound healing. Medicinal plants play a great role in the wound healing process. In recent decades, many studies have focused on the development of novel wound dressings that incorporate medicinal plant extracts or their purified active compounds, which are potential alternatives to conventional wound dressings. Several studies have also investigated the mechanism of action of various herbal medicines in wound healing process. This paper attempts to highlight and review the mechanistic perspective of wound healing mediated by plant-based natural products. The findings showed that herbal medicines act through multiple mechanisms and are involved in various stages of wound healing. Some herbal medicines increase the expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β (TGF-β) which play important role in stimulation of re-epithelialization, angiogenesis, formation of granulation tissue, and collagen fiber deposition. Some other wound dressing containing herbal medicines act as inhibitor of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) protein expression thereby inducing antioxidant and anti-inflammatory properties in various phases of the wound healing process. Besides the growing public interest in traditional and alternative medicine, the use of herbal medicine and natural products for wound healing has many advantages over conventional medicines, including greater effectiveness due to diverse mechanisms of action, antibacterial activity, and safety in long-term wound dressing usage.
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Affiliation(s)
- Omid Yazarlu
- Mashhad University of Medical Sciences, Department of General Surgery, Mashhad, Iran
| | - Mehrdad Iranshahi
- Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
| | | | - Sara Reshadat
- Department of Internal Medicine, Semnan University of Medical Sciences, Semnan, Iran
| | - Solomon Habtemariam
- Pharmacognosy Research Laboratories and Herbal Analysis Services UK, University of Greenwich, Central Avenue, Chatham-Maritime, Kent ME4 4TB, UK
| | - Milad Iranshahy
- Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
| | - Maede Hasanpour
- Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
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Akinola BK, Abiodun AA. Intervertebral Disk Degeneration in Rabbits: Histomorphometric and Histomorphological Effects of Aloe Vera Gel. Global Spine J 2021; 11:852-858. [PMID: 32677517 PMCID: PMC8258827 DOI: 10.1177/2192568220927712] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 12/02/2022] Open
Abstract
STUDY DESIGN This is an experimental study using an animal model. OBJECTIVES Disk degeneration is a common cause of low back pain. However, few attempts have been made to proffer a medical solution. The aim of this study was to investigate the effect of aloe vera gel (AVG) on the histomorphometric changes in the intervertebral disk of annular-punctured rabbits. METHODS A total of 25 rabbits weighing 1.0 to 3.5 kg were used for this study; 20 rabbits were subjected to annular puncture of the L3/L4, L4/L5, and L5/L6 disks using an 18G needle. Five rabbits were randomly assigned to 5 groups (A, B, C, D, and E) of 5 animals per group. Group A was not punctured. Group B was punctured. Groups C, D, and E were punctured and given 600 400, and 200 (mg/kg) of AVG orally, respectively. The disk histology and nucleus pulposus cell count were done 6 weeks after the puncture procedure. RESULTS The results revealed a gradual reversal of degenerative changes in the treated groups compared with the nontreated groups (P < .05). The observed changes in the organization of the elastic and collagen content, increase in fibrochondrocyte-like cells of the nucleus pulposus and annulus fibrosus (P = .0027), and the degree of degeneration of the disk (P = .0001) in the treated groups compared with the nontreated groups were statistically significant. CONCLUSION Administration of AVG halted and reversed disk degeneration in an annular puncture-induced disk degeneration rabbit model.
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Affiliation(s)
- Busuyi Kolade Akinola
- Federal University of Technology, Akure, Nigeria,Busuyi Kolade Akinola, Department of Human Anatomy, Federal University of Technology, Akure, Ondo +234, Nigeria.
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Poordast T, Ghaedian L, Ghaedian L, Najib FS, Alipour S, Hosseinzadeh M, Vardanjani HM, Salehi A, Hosseinimehr SJ. Aloe Vera; A new treatment for atrophic vaginitis, A randomized double-blinded controlled trial. JOURNAL OF ETHNOPHARMACOLOGY 2021; 270:113760. [PMID: 33383112 DOI: 10.1016/j.jep.2020.113760] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/19/2020] [Revised: 12/20/2020] [Accepted: 12/23/2020] [Indexed: 06/12/2023]
Abstract
AIM OF THE STUDY Vaginal atrophy is of the most common problems during menopause with significant psychosocial and medical consequences. Estrogen as an approved therapy for vaginal atrophy can be associated with adverse effects and several contraindications in menopause patients. The aim is to compare the effect of Aloe Vera vaginal cream with commercially available estrogen vaginal cream for management of vaginal atrophy in menopause females. MATERIALS AND METHODS This is a double-blinded randomized controlled trial on 60menopause female with complaints of vaginal atrophy symptoms. Subjects were randomly allocated in two groups of 30 patients, named as estrogen and Aloe Vera groups. Vaginal health index (VHI), maturity value (MV), vaginal cytologic smear, transvaginal sonography (TVS) and severity of symptoms related to vaginal atrophy were assessed before and after 6-weeks of vaginal cream administration. RESULTS Comparison of MV before and after treatment revealed that superficial cells were significantly increased after administration of both vaginal cream (6.67 VS 54.33 in Aloe Vera group; 4.33 VS 59.67 in estrogen group). In addition, VHI (13.83 vs 20.13 in Aloe Vera group; 13.97 vs 19.93 in estrogen group) and symptoms of vaginal atrophy (3.63 vs 1.10 in Aloe Vera group; 3.90 vs 0.66 in estrogen groups) were also significantly improved after treatment in both groups. There was no significant difference between groups after treatment except for fluid volume with a superiority in Aloe Vera group (P-value = 0.004) CONCLUSION: Aloe Vera vaginal cream can be as effective as estrogen vaginal cream in the management of vaginal atrophy in menopause females.
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Affiliation(s)
- Tahereh Poordast
- Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
| | - Lida Ghaedian
- Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
| | - Leila Ghaedian
- Department of Obstetrics and Gynecology, Kosar Hospital, Shiraz, Iran
| | - Fatemeh Sadat Najib
- Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
| | - Shohreh Alipour
- Department of Quality Control, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
| | - Massood Hosseinzadeh
- Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
| | | | - Alireza Salehi
- Research Center for Traditional Medicine and History of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
| | - Seyed Jalal Hosseinimehr
- Department of Radiopharmacy, Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran
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Polyvinyl alcohol (PVA) mixed green–clay and aloe vera based polymeric membrane optimization: Peel-off mask formulation for skin care cosmeceuticals in green nanotechnology. J Mol Struct 2021. [DOI: 10.1016/j.molstruc.2020.129592] [Citation(s) in RCA: 16] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/23/2022]
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Gans I, El Abiad JM, James AW, Levin AS, Morris CD. Administration of TGF-ß Inhibitor Mitigates Radiation-induced Fibrosis in a Mouse Model. Clin Orthop Relat Res 2021; 479:468-474. [PMID: 33252888 PMCID: PMC7899598 DOI: 10.1097/corr.0000000000001286] [Citation(s) in RCA: 9] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 09/25/2019] [Accepted: 04/14/2020] [Indexed: 02/06/2023]
Abstract
BACKGROUND Radiation-induced fibrosis is a long-term adverse effect of external beam radiation therapy for cancer treatment that can cause pain, loss of function, and decreased quality of life. Transforming growth factor beta (TGF-β) is believed to be critical to the development of radiation-induced fibrosis, and TGF-β inhibition decreases the development of fibrosis. However, no treatment exists to prevent radiation-induced fibrosis. Therefore, we aimed to mitigate the development of radiation-induced fibrosis in a mouse model by inhibiting TGF-β. QUESTION/PURPOSES Does TGF-β inhibition decrease the development of muscle fibrosis induced by external beam radiation in a mouse model? METHODS Twenty-eight 12-week-old male C57BL/6 mice were assigned randomly to three groups: irradiated mice treated with TGF-βi, irradiated mice treated with placebo, and control mice that received neither irradiation nor treatment. The irradiated mice received one 50-Gy fraction of radiation to the right hindlimb before treatment initiation. Mice treated with TGF-c (n = 10) received daily intraperitoneal injections of a small-molecule inhibitor of TGF-β (1 mg/kg) in a dimethyl sulfoxide vehicle for 8 weeks (seven survived to histologic analysis). Mice treated with placebo (n = 10) received daily intraperitoneal injections of only a dimethyl sulfoxide vehicle for 8 weeks (10 survived to histologic analysis). Control mice (n = 8) received neither radiation nor TGF-β treatment. Control mice were euthanized at 3 months because they were not expected to exhibit any changes related to treatment. Mice in the two treatment groups were euthanized 9 months after radiation, and the quadriceps of each thigh was sampled. Masson's trichome stain was used to assess muscle fibrosis. Slides were viewed at 10 × magnification using bright-field microscopy, and in a blinded fashion, five representative images per mouse were used to quantify fibrosis. The mean ± SD fibrosis pixel densities in the TGF-βi and radiation-only groups were compared using Mann-Whitney U tests. The ratio of fibrosis to muscle was calculated using the mean fibrosis per slide in the TGF-βi group to standardize measurements. Alpha was set at 0.05. RESULTS The mean (± SD) percentage of fibrosis per slide was greater in the radiation-only group (1.2% ± 0.42%) than in the TGF-βi group (0.14% ± 0.09%) (odds ratio 0.12 [95% CI 0.07 to 0.20]; p < 0.001). Among control mice, mean fibrosis was 0.05% ± 0.02% per slide. Mice in the radiation-only group had 9.1 times the density of fibrosis as did mice in the TGF-βi group. CONCLUSION Our study provides preliminary evidence that the fibrosis associated with radiation therapy to a quadriceps muscle can be reduced by treatment with a TGF-β inhibitor in a mouse model. CLINICAL RELEVANCE If these observations are substantiated by further investigation into the role of TGF-β inhibition on the development of radiation-induced fibrosis in larger animal models and humans, our results may aid in the development of novel therapies to mitigate this complication of radiation treatment.
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Affiliation(s)
- Itai Gans
- I. Gans, J. M. El Abiad, A. S. Levin, C. D. Morris, Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
- A. W. James, Department of Pathology, The Johns Hopkins University School of Medicine, Ross Research Building, Baltimore, MD, USA
- C. D. Morris, Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
- The institution of one or more of the authors (IG) has received, during the study period, funding from the Orthopaedic Research and Education Foundation (Rosemont, IL, USA)
| | - Jad M El Abiad
- I. Gans, J. M. El Abiad, A. S. Levin, C. D. Morris, Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
- A. W. James, Department of Pathology, The Johns Hopkins University School of Medicine, Ross Research Building, Baltimore, MD, USA
- C. D. Morris, Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
- The institution of one or more of the authors (IG) has received, during the study period, funding from the Orthopaedic Research and Education Foundation (Rosemont, IL, USA)
| | - Aaron W James
- I. Gans, J. M. El Abiad, A. S. Levin, C. D. Morris, Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
- A. W. James, Department of Pathology, The Johns Hopkins University School of Medicine, Ross Research Building, Baltimore, MD, USA
- C. D. Morris, Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
- The institution of one or more of the authors (IG) has received, during the study period, funding from the Orthopaedic Research and Education Foundation (Rosemont, IL, USA)
| | - Adam S Levin
- I. Gans, J. M. El Abiad, A. S. Levin, C. D. Morris, Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
- A. W. James, Department of Pathology, The Johns Hopkins University School of Medicine, Ross Research Building, Baltimore, MD, USA
- C. D. Morris, Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
- The institution of one or more of the authors (IG) has received, during the study period, funding from the Orthopaedic Research and Education Foundation (Rosemont, IL, USA)
| | - Carol D Morris
- I. Gans, J. M. El Abiad, A. S. Levin, C. D. Morris, Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
- A. W. James, Department of Pathology, The Johns Hopkins University School of Medicine, Ross Research Building, Baltimore, MD, USA
- C. D. Morris, Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
- The institution of one or more of the authors (IG) has received, during the study period, funding from the Orthopaedic Research and Education Foundation (Rosemont, IL, USA)
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21
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Imran M, Sharma JN, Kamal M, Asif M. Standardization and Wound-Healing Activity of Petroleum, Ethanolic and Aqueous Extracts of Ficus Benghalensis Leaves. Pharm Chem J 2021. [DOI: 10.1007/s11094-021-02319-x] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/22/2022]
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Panahi Y, Rastgar N, Zamani A, Sahebkar A. Comparing the Therapeutic Effects of Aloe vera and Olive Oil Combination Cream versus Topical Betamethasone for Atopic Dermatitis: A Randomized Double-blind Clinical Trial. J Pharmacopuncture 2020; 23:173-178. [PMID: 33072415 PMCID: PMC7540232 DOI: 10.3831/kpi.2020.23.3.173] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/28/2018] [Revised: 07/31/2020] [Accepted: 08/28/2020] [Indexed: 11/09/2022] Open
Abstract
Objectives Atopic dermatitis (AD) is a prevalent and chronic, pruritic inflammatory skin condition that can influence all age groups. AD is associated with a poor health-related quality of life. This randomized clinical trial was performed to compare the effectiveness of Olivederma (combination of aloe vera and virgin olive oil) or betamethasone regarding disease severity, quality of life, serum IgE and eosinophil count. Methods Thirty-six AD patients were randomly allocated to topical Olivederma or betamethasone, and were followed for 6 weeks. Results Total SCORAD severity scores showed significant decrease in both groups, while it was more prominent in Olivederma group (64.5% improvement in Olivederma vs. 13.5% improvement in Betamethasone, p-value < 0.001). Quality of life (DLQI questionnaire) of AD patients was significantly improved after 6 weeks treatment with Betamethasone (22.3%, p < 0.001) and Olivederma (60.7%, p-value < 0.001). Olivederma group showed a significantly lower DLQI score in comparison with Betamethasone treated patients after 6 weeks of therapy (p < 0.001). Improvements in eosinophil count and serum IgE was observed. Conclusion In summary, this study shows that Olivederma is superior to topical Betamethasone after 6 weeks of therapy with regard to disease severity, quality of life and eosinophil count.
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Affiliation(s)
- Yunes Panahi
- Pharmacotherapy Department, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran
| | - Nassim Rastgar
- Department of Pharmaceutics, Faculty of Pharmacy, Pharmaceutical Science Branch, Islamic Azad University, Tehran, Iran
| | - Ali Zamani
- Pharmacotherapy Department, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran
| | - Amirhossein Sahebkar
- Halal Research Center of IRI, FDA, Tehran, Iran.,Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.,Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.,Polish Mother's Memorial Hospital Research Institute (PMMHRI), Lodz, Poland
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Alkhouli M, Laflouf M, Alhaddad M. Efficacy of Aloe-Vera Use for Prevention of Chemotherapy-Induced Oral Mucositis in Children with Acute Lymphoblastic Leukemia: A Randomized Controlled Clinical Trial. Compr Child Adolesc Nurs 2020; 44:49-62. [PMID: 32101488 DOI: 10.1080/24694193.2020.1727065] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/24/2022]
Abstract
Oral mucositis can be caused by chemotherapy and can affect a patient's quality of life. Nowadays, to prevent chemotherapy-induced oral mucositis (CIOM) is a crucial point in palliative care centers. This trial aimed to assess the effectiveness of aloe-vera in that concept. The trial was accomplished at Hematology Department of Hospital of Children of Damascus University, Syria. Acute lymphoblastic leukemia (ALL) children were the population from which 26 children were enrolled in the study. They were aged between 3 and 6 years old and were randomly referred according to the intervention into two groups, Aloe-vera (AV) and sodium bicarbonate 5% (13 each). Spongeous sticks were used to help in applying the material on tongue, labial and buccal mucosa, lips, floor of the mouth, and hard palate. Two blinded external examiners evaluated oral mucosa weekly for up to 2 months using the World Health Organization grading scale. Mann-Whitney U test was used to analyze data. According to the observed findings, CIOM degrees were less severe in the aloe-vera group than in the sodium bicarbonate group. Statistically significant difference of occurrence of different CIOM degrees between groups was recorded in the 2nd, 3rd, 4th, and 7th weeks of follow-up period. Moreover, Mann-Whitney U test indicated that patients in the sodium bicarbonate group began CIOM sooner than those in the aloe-vera group with a statistically significant difference (p = .001). These findings show that topical application of aloe-vera solution is effective in the prevention of CIOM in ALL children.
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Affiliation(s)
- Muaaz Alkhouli
- Department of Pediatric Dentistry, Faculty of Dentistry, Damascus University, Damascus, Syria
| | - Mohannad Laflouf
- Department of Pediatric Dentistry, Faculty of Dentistry, Damascus University, Damascus, Syria
| | - Mazen Alhaddad
- Department of Pediatrics, Faculty of Medicine, Damascus University, Damascus, Syria
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Iacopetti I, Perazzi A, Martinello T, Gemignani F, Patruno M. Hyaluronic acid, Manuka honey and Acemannan gel: Wound-specific applications for skin lesions. Res Vet Sci 2020; 129:82-89. [PMID: 31954318 DOI: 10.1016/j.rvsc.2020.01.009] [Citation(s) in RCA: 19] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/12/2019] [Revised: 01/09/2020] [Accepted: 01/10/2020] [Indexed: 12/11/2022]
Abstract
Healing of open wounds is of great medical importance. Wound healing is a complex process that aims to restore the function and structure of damaged tissue. This study was conducted to compare secondary intention healing of wounds treated daily with a topical application of commercially available hyaluronic acid (HA), Manuka honey (MH), Acemannan gel (AG), or a placebo. Bilateral wounds were surgically created on the backs of six sheep. At two and six weeks post-wound creation, biopsies were obtained to perform histological, immunohistochemical, and molecular analyses of the wound site. Daily clinical evaluations were performed and weekly photographs were taken of the wounds. HA treatment promoted a physiological progression of the healing process in all wound healing phases, while stimulating an abundant cutaneous adnexa and promoting rapid healing, representing the most compelling treatment. MH-treated wounds were slightly dry. However, the main effect of MH was to promote cell proliferation and neovascularization, with an overall pro-inflammatory effect. Results suggest that MH treatment enhances the healing process. AG treatment dehydrated the wounds and stimulated late granulation tissue and cell proliferation. Moreover, AG-treated wounds produced a mild late pro-inflammatory and neovascularization effect. Our data indicate that AG treatment can have a positive influence on moist wounds with abundant granulation tissue and exudate.
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Affiliation(s)
- I Iacopetti
- Department of Animal Medicine, Production and Health, University of Padova, viale dell'Università 16, 35020 Legnaro-Agripolis, Padova, Italy
| | - A Perazzi
- Department of Animal Medicine, Production and Health, University of Padova, viale dell'Università 16, 35020 Legnaro-Agripolis, Padova, Italy
| | - T Martinello
- Department of Comparative Biomedicine and Food Science, University of Padova, viale dell'Università 16, 35020 Legnaro-Agripolis, Padova, Italy
| | - F Gemignani
- Private practitioner, Winchester, United Kingdom
| | - M Patruno
- Department of Comparative Biomedicine and Food Science, University of Padova, viale dell'Università 16, 35020 Legnaro-Agripolis, Padova, Italy.
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Palmieri B, Vadalà M, Laurino C. Nutrition in wound healing: investigation of the molecular mechanisms, a narrative review. J Wound Care 2019; 28:683-693. [DOI: 10.12968/jowc.2019.28.10.683] [Citation(s) in RCA: 23] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/17/2023]
Abstract
Nutrition can be outlined in terms of epigenetic signals influencing each of the wound healing steps (haemostasis, inflammatory, proliferative and remodelling phase). Specific nutrients, such as amino acids, minerals, vitamins, natural compounds and herbal extracts, target DNA-regulating transcription factors, cytokines, extracellular matrix proteins and glycosaminoglycan, and are specifically involved in the wound healing process. This review focuses on experimental in vivo and clinical evidence of dietary supplements administration in pressure ulcers. A good nutritional status is, for example, fundamental to the haemostasis phase of skin wounds. In the inflammatory phase, vitamin A enhances cytokine release, bromelain and amino acids prevent prolonged inflammatory events, while vitamin C enhances neutrophil migration and lymphocyte activation. In the proliferative phase, vitamin C and Centella asiatica are required for collagen synthesis. Glucosamine enhances hyaluronic acid production, vitamin A promotes epithelial cell differentiation, zinc is required for DNA and protein synthesis and cell division, and Aloe vera supports granulation tissue generation. Finally, in the remodelling phase, amino acids and proteins play a key role in wound scar stabilisation.
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Affiliation(s)
- Beniamino Palmieri
- Dipartimento Chirurgico, Medico, Odontoiatrico e di Scienze Morfologiche con Interesse Trapiantologico, Oncologico e di Medicina Rigenerativa, Università degli Studi di Modena e Reggio Emilia, Modena, Italy. Second Opinion Medical Network
| | - Maria Vadalà
- Dipartimento Chirurgico, Medico, Odontoiatrico e di Scienze Morfologiche con Interesse Trapiantologico, Oncologico e di Medicina Rigenerativa, Università degli Studi di Modena e Reggio Emilia, Modena, Italy. Second Opinion Medical Network
| | - Carmen Laurino
- Dipartimento Chirurgico, Medico, Odontoiatrico e di Scienze Morfologiche con Interesse Trapiantologico, Oncologico e di Medicina Rigenerativa, Università degli Studi di Modena e Reggio Emilia, Modena, Italy. Second Opinion Medical Network
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Borrelli MR, Shen AH, Lee GK, Momeni A, Longaker MT, Wan DC. Radiation-Induced Skin Fibrosis: Pathogenesis, Current Treatment Options, and Emerging Therapeutics. Ann Plast Surg 2019; 83:S59-S64. [PMID: 31513068 PMCID: PMC6746243 DOI: 10.1097/sap.0000000000002098] [Citation(s) in RCA: 100] [Impact Index Per Article: 16.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/21/2022]
Abstract
Radiotherapy (RT) has become an indispensable part of oncologic treatment protocols for a range of malignancies. However, a serious adverse effect of RT is radiodermatitis; almost 95% of patients develop moderate to severe skin reactions following radiation treatment. In the acute setting, these can be erythema, desquamation, ulceration, and pain. Chronically, soft tissue atrophy, alopecia, and stiffness can be noted. Radiodermatitis can delay oncologic treatment protocols and significantly impair quality of life. There is currently a paucity of effective treatment options and prevention strategies for radiodermatitis. Importantly, recent preclinical and clinical studies have suggested that fat grafting may be of therapeutic benefit, reversing detrimental changes to soft tissue following RT. This review outlines the damaging effects of RT on the skin and soft tissue as well as discusses available treatment options for radiodermatitis. Emerging strategies to mitigate detrimental, chronic radiation-induced changes are also presented.
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Affiliation(s)
- Mimi R. Borrelli
- Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic Surgery, Stanford University School of Medicine, Stanford, California
| | - Abra H. Shen
- Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic Surgery, Stanford University School of Medicine, Stanford, California
| | - Gordon K. Lee
- Division of Plastic and Reconstructive Surgery, Stanford University Medical Center, Palo Alto, California
| | - Arash Momeni
- Division of Plastic and Reconstructive Surgery, Stanford University Medical Center, Palo Alto, California
| | - Michael T. Longaker
- Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic Surgery, Stanford University School of Medicine, Stanford, California
- Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California
| | - Derrick C. Wan
- Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic Surgery, Stanford University School of Medicine, Stanford, California
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Subramani K, Kolathupalayam Shanmugam B, Rangaraj S, Palanisamy M, Periasamy P, Venkatachalam R. Screening the UV-blocking and antimicrobial properties of herbal nanoparticles prepared from Aloe vera leaves for textile applications. IET Nanobiotechnol 2019; 12:459-465. [PMID: 29768230 DOI: 10.1049/iet-nbt.2017.0097] [Citation(s) in RCA: 12] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/19/2022] Open
Abstract
Nanomaterials play a vital role in textile industries due to their unique properties and applications. There is an increase in the use of nanoscale phyto products in textiles to control the bacterial infection in fabrics. Here, natural herbal nanoparticles of different sizes were prepared from shade-dried Aloe vera plant leaves using ball milling technique without any additives. The amorphous herbal A. vera nanoparticles possess an average particle size of 40 ± 2 nm and UV-absorption maximum at 269 nm. A. vera nanopowders-chitosan nanocomposites were prepared and coated on cotton fabrics using pad-dry cure method. The evaluation of antibacterial activity against Escherichia coli (22.05 ± 0.06 mm) and Staphylococcus aureus (27.17 ± 0.02 mm), UV-protection properties (UV-protection factor = 57.2 ± 0.1), and superhydrophobic nature (155 ± 3°) of the prepared herbal nanoparticles and their composites were analysed by disc diffusion, UV-visible spectral analysis, and contact angle analysis. Understanding the functional properties of herbal nanoparticles, coated particles on fabrics highlights their potential applications in protective clothing with better antimicrobial properties, hydrophobicity, and UV-protection properties. This study of using A. vera herbal nanoparticles in textiles significantly enhances the fabric performance to develop protective textile fabrics in defence and biomedical fields.
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Affiliation(s)
- Karthik Subramani
- Centre for Nano Science and Technology, K.S. Rangasamy College of Technology, Tiruchengode 637215, Tamil Nadu, India
| | - Balu Kolathupalayam Shanmugam
- Centre for Nano Science and Technology, K.S. Rangasamy College of Technology, Tiruchengode 637215, Tamil Nadu, India
| | - Suriyaprabha Rangaraj
- Centre for Nano Science and Technology, K.S. Rangasamy College of Technology, Tiruchengode 637215, Tamil Nadu, India
| | - Manivasakan Palanisamy
- Centre for Nano Science and Technology, K.S. Rangasamy College of Technology, Tiruchengode 637215, Tamil Nadu, India
| | - Prabu Periasamy
- Centre for Nano Science and Technology, K.S. Rangasamy College of Technology, Tiruchengode 637215, Tamil Nadu, India
| | - Rajendran Venkatachalam
- Centre for Nano Science and Technology, K.S. Rangasamy College of Technology, Tiruchengode 637215, Tamil Nadu, India.
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Tyliszczak B, Drabczyk A, Kudłacik-Kramarczyk S, Rudnicka K, Gatkowska J, Sobczak-Kupiec A, Jampilek J. In vitro biosafety of pro-ecological chitosan-based hydrogels modified with natural substances. J Biomed Mater Res A 2019; 107:2501-2511. [PMID: 31298778 DOI: 10.1002/jbm.a.36756] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/12/2019] [Revised: 07/05/2019] [Accepted: 07/08/2019] [Indexed: 12/15/2022]
Abstract
Hydrogels belong to the group of materials with growing interest on the market of polymers. In this article, hydrogels based on Beetosan were obtained using ultraviolet (UV) radiation. Main component of hydrogel matrix-Beetosan-is chitosan obtained from naturally died honeybees. Such hydrogels were modified with active substances, that is, caffeine, bee pollen, Salvia officinalis (sage), and Aloe vera juice. Next, the analysis of cytotoxicity of hydrogels in relation to murine fibroblasts by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide and neutral red uptake assays were conducted. Furthermore, surface morphology, tensile strength, geometry, and roughness of hydrogels were characterized. Hydrogels did not show cytotoxicity to recommended L929 murine fibroblasts. These polymers did not affect adversely the growth and viability of these cells. Moreover, Beetosan hydrogels were characterized by flexibility as well as by diversified surface morphology that could indicate their high absorbency. Therefore these materials may be considered as useful for biomedical purposes with special emphasis on application as modern wound dressings that not only absorb wound exudate but also contain natural substances with therapeutic properties that is beneficial from the point of view of wound healing process.
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Affiliation(s)
- Bożena Tyliszczak
- Faculty of Chemical Engineering and Technology, Department of Chemistry and Technology of Polymers, Cracow University of Technology, Krakow, Poland
| | - Anna Drabczyk
- Faculty of Chemical Engineering and Technology, Department of Chemistry and Technology of Polymers, Cracow University of Technology, Krakow, Poland
| | - Sonia Kudłacik-Kramarczyk
- Faculty of Chemical Engineering and Technology, Department of Chemistry and Technology of Polymers, Cracow University of Technology, Krakow, Poland
| | - Karolina Rudnicka
- Faculty of Biology and Environmental Protection, Department of Immunology and Infectious Biology, University of Lodz, Łódź, Poland
| | - Justyna Gatkowska
- Faculty of Biology and Environmental Protection, Department of Immunoparasitology University of Lodz, Łódź, Poland
| | - Agnieszka Sobczak-Kupiec
- Faculty of Chemical Engineering and Technology, Institute of Inorganic Chemistry and Technology, Cracow University of Technology, Krakow, Poland
| | - Josef Jampilek
- Faculty of Natural Sciences, Department of Analytical Chemistry, Comenius University, Bratislava, Slovakia.,Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacky University, Olomouc, Czech Republic
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Bassino E, Gasparri F, Munaron L. Natural dietary antioxidants containing flavonoids modulate keratinocytes physiology: In vitro tri-culture models. JOURNAL OF ETHNOPHARMACOLOGY 2019; 238:111844. [PMID: 30940568 DOI: 10.1016/j.jep.2019.111844] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/14/2019] [Revised: 03/21/2019] [Accepted: 03/27/2019] [Indexed: 06/09/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Several traditional medicinal herbs are widely used for dermatologic and cosmetic preparations. The beneficial skin repair activity is detected in various phases of wound-healing process, such as cell-cell, cell-matrix interactions or collagen synthesis. AIM OF THE STUDY The study assessed the effects of Opuntia ficus-indica (L.) Mill. (Opuntia) and Milk Thistle (MT) (Silybum marianum (L.) Gaerth) on adult keratinocytes (HaCaT) functioning under basal condition or in the presence of mechanical damage (wounded cells). MATERIALS AND METHODS The role of the natural compounds was tested on HaCaT grown in mono-culture and tri-culture configurations. In tri-cultures models, HaCaT were treated with the conditioned media (CM) obtained by Human Normal Dermal Fibroblast (NHDF) and Human Dermal Microvascular Endothelial cells (HMVEC) co-cultures. Specifically, were tested cell viability, oxidative stress mechanisms (cytokines release and lipid peroxidation) and cellular remodelling (growth factors release or metalloproteinase modulation). Moreover, the migratory potential of HaCaT was analysed by the use of wound healing in vitro assay. RESULTS Opuntia and MT differently modified the metabolism (EGF, MMP-9), and the migratory properties of HaCaT both under physiological conditions or upon mechanical damage (wounded cells). Moreover, both compounds modulated HaCaT response to oxidative stress. The response to the natural compounds were modified, and in some cases potentiated, in tri-culture configuration systems. CONCLUSIONS The data demonstrated that in vitro tri-culture approach is suitable to characterize the role of natural compounds on the complex communication between dermal-epidermal cellular components and microvascular endothelium. Specifically, Opuntia and MT are good alternatives to synthetic compounds in skin repair promotion.
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Affiliation(s)
- Eleonora Bassino
- Department of Life Sciences and Systems Biology, University of Torino, Italy.
| | | | - Luca Munaron
- Department of Life Sciences and Systems Biology, University of Torino, Italy
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Wound Healing Property of Curcuminoids as a Microcapsule-Incorporated Cream. Pharmaceutics 2019; 11:pharmaceutics11050205. [PMID: 31052413 PMCID: PMC6572040 DOI: 10.3390/pharmaceutics11050205] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/01/2019] [Revised: 03/23/2019] [Accepted: 03/29/2019] [Indexed: 01/22/2023] Open
Abstract
Curcuminoids have been used for the management of burns and wound healing in traditional Chinese medicine practices but the wide application of curcuminoids as a healing agent for wounds has always been a known problem due to their poor solubility, bioavailability, colour staining properties, as well as due to their intense photosensitivity and the need for further formulation approaches to maximise their various properties in order for them to considerably contribute towards the wound healing process. In the present study, a complex coacervation microencapsulation was used to encapsulate curcuminoids using gelatin B and chitosan. This study also focused on studying and confirming the potential of curcuminoids in a microencapsulated form as a wound healing agent. The potential of curcuminoids for wound management was evaluated using an in vitro human keratinocyte cell (HaCaT) model and the in vivo heater-inflicted burn wound model, providing evidence that the antioxidant activities of both forms of curcuminoids, encapsulated or not, are higher than those of butylated hydroxyanisole and butylated hydroxytoluene in trolox equivalent antioxidant capacity (TEAC) and (2,2-diphenyl-1-picryl-hydrazyl-hydrate) (DPPH) studies. However, curcuminoids did not have much impact towards cell migration and proliferation in comparison with the negative control in the in vitro HaCaT study. The micoencapsulation formulation was shown to significantly influence wound healing in terms of increasing the wound contraction rate, hydroxyproline synthesis, and greater epithelialisation, which in turn provides strong justification for the incorporation of the microencapsulated formulation of curcuminoids as a topical treatment for burns and wound healing management as it has the potential to act as a crucial wound healing agent in healthcare settings.
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31
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Al-Maweri SA, Ashraf S, Lingam AS, Alqutaibi A, Abdulrab S, Alaizari N, Halboub E. Aloe vera in treatment of oral submucous fibrosis: A systematic review and meta-analysis. J Oral Pathol Med 2018; 48:99-107. [DOI: 10.1111/jop.12789] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/18/2018] [Accepted: 10/10/2018] [Indexed: 12/20/2022]
Affiliation(s)
- Sadeq Ali Al-Maweri
- Department of Oral Medicine and Diagnostic Science; Al-Farabi Colleges; Riyadh Saudi Arabia
| | - Sajna Ashraf
- Department of Oral Medicine and Diagnostic Science; Al-Farabi Colleges; Riyadh Saudi Arabia
| | - Amara Swapna Lingam
- Department of Oral Medicine and Diagnostic Science; Al-Farabi Colleges; Riyadh Saudi Arabia
| | - Ahmed Alqutaibi
- Department of Prosthodontics; Ahram Canadian University; Cairo Egypt
| | - Saleem Abdulrab
- Department of Restorative Dental Sciences; Al-Farabi Colleges; Riyadh Saudi Arabia
- Primary Health Care Corporation; Doha Qatar
| | - Nader Alaizari
- Department of Oral Medicine and Diagnostic Science; Al-Farabi Colleges; Riyadh Saudi Arabia
| | - Esam Halboub
- Department of Maxillofacial Surgery and Diagnostic Sciences; College of Dentistry; Jazan University; Jazan Saudi Arabia
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Veeruraj A, Liu L, Zheng J, Wu J, Arumugam M. Evaluation of astaxanthin incorporated collagen film developed from the outer skin waste of squid Doryteuthis singhalensis for wound healing and tissue regenerative applications. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2018; 95:29-42. [PMID: 30573252 DOI: 10.1016/j.msec.2018.10.055] [Citation(s) in RCA: 41] [Impact Index Per Article: 5.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/24/2018] [Revised: 08/26/2018] [Accepted: 10/15/2018] [Indexed: 11/26/2022]
Abstract
The present investigation was aimed to evaluate in vivo wound healing activity of astaxanthin incorporated collagen hydrogel film biomaterials extracted from the outer skin waste of squid Doryteuthis singhalensis, to releases antibiotic, delivering potentialities of excisional and incisional wound model in Wistar rats. These results suggested that the astaxanthin incorporated collagen film (ACF) and gentamicin incorporated collagen film (GCF) exhibited excellent wound healing activity (71%) in both full thickness excision and linear incision in rats. The in-vitro antioxidant abilities of extracted astaxanthin exhibited strongly significant 1,1‑diphenyl‑2‑picrylhydrazyl (DPPH) radical scavenging activity. In addition, tensile strength, epithelialization, hydroxyproline content and protein content in ACF and GCF treated groups were significantly increased. Histopathological assessment revealed an increase in collagen content, fibroblasts, granulation, thickness of scar formation, effective neovascularization and faster epithelialization within the short duration after the treatment of ACF and GCF compared to the control groups. The structure of prepared ACF and GCF biomaterials were characterized by SEM, EDS, and XRD. The in vivo biological study of the collagen-based film releases the antibiotic substance. The composite of collagen based biomaterials displays a promising biocompatibility through the dermal wound healing process as well as an evidence of biodegradability. Thus, the marine-derived biomaterials gave a substantial pledge for the development of biodegradable materials in drug delivery and soft tissue regeneration process.
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Affiliation(s)
- Anguchamy Veeruraj
- Fuli Institute of Food Science (FIFS), College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China; Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamilnadu, India; ZJU-UA Joint Lab for Molecular Nutrition and Bioactive Peptides, College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China.
| | - Ling Liu
- Fuli Institute of Food Science (FIFS), College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China
| | - Jiexia Zheng
- Fuli Institute of Food Science (FIFS), College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China
| | - Jianping Wu
- Fuli Institute of Food Science (FIFS), College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China; ZJU-UA Joint Lab for Molecular Nutrition and Bioactive Peptides, College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China; Department of Agricultural, Food and Nutritional Science, 4-10 Ag/For Building, University of Alberta, Edmonton, Alberta T6G 2P5, Canada
| | - Muthuvel Arumugam
- Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamilnadu, India
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Drudi D, Tinto D, Ferranti D, Fiorelli F, Pozzo MD, Capitani O. Aloe barbadensis miller versus silver sulfadiazine creams for wound healing by secondary intention in dogs and cats: A randomized controlled study. Res Vet Sci 2017; 117:1-9. [PMID: 29131970 DOI: 10.1016/j.rvsc.2017.10.010] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/21/2017] [Revised: 08/25/2017] [Accepted: 10/29/2017] [Indexed: 11/18/2022]
Abstract
The objective of this study was to compare the effectiveness of the topical application of Aloe barbadensis Miller (juice and fresh gel) to skin wounds, in dogs and cats, with that of topically applied silver sulfadiazine cream. The sample included 16 patients with cutaneous wounds (13 dogs and three cats) that were divided into three groups. Aloe vera "juice" and "fresh gel" were applied for groups I and II, respectively, while silver sulfadiazine was applied for the control group III. In order to evaluate the healing of wounds, the following parameters were taken into consideration: the percentage of wound shrinkage, the healing time and the macroscopic appearance of the scarring process. The interpretation of the data relating to the percentage of wound shrinkage showed a faster rate for groups I and II compared to that of group III. However, the wound shrinkage rates between "juice" and "fresh gel" protocols were not significantly different. Aloe vera was more effective than silver sulfadiazine, in accelerating wound shrinkage, reducing healing time and decreasing the severity of the associated injuries.
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Affiliation(s)
- Dario Drudi
- Clinica veterinaria Nervianese, Via Giulio Cesare Lampugnani 3, Milan, Nerviano, Italy.
| | - Debora Tinto
- Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Bologna, Ozzano, Italy
| | | | | | | | - Ombretta Capitani
- Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Bologna, Ozzano, Italy
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Naseri-Nosar M, Farzamfar S, Salehi M, Vaez A, Tajerian R, Azami M. Erythropoietin/aloe vera-releasing wet-electrospun polyvinyl alcohol/chitosan sponge-like wound dressing: In vitro and in vivo studies. J BIOACT COMPAT POL 2017. [DOI: 10.1177/0883911517731793] [Citation(s) in RCA: 25] [Impact Index Per Article: 3.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/03/2023]
Affiliation(s)
- Mahdi Naseri-Nosar
- Department of Tissue Engineering & Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
| | - Saeed Farzamfar
- Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
| | - Majid Salehi
- Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
| | - Ahmad Vaez
- Department of Tissue Engineering & Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
| | - Roksana Tajerian
- Department of Tissue Engineering & Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
| | - Mahmoud Azami
- Department of Tissue Engineering & Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
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Vallée A, Lecarpentier Y, Guillevin R, Vallée JN. Interactions between TGF-β1, canonical WNT/β-catenin pathway and PPAR γ in radiation-induced fibrosis. Oncotarget 2017; 8:90579-90604. [PMID: 29163854 PMCID: PMC5685775 DOI: 10.18632/oncotarget.21234] [Citation(s) in RCA: 147] [Impact Index Per Article: 18.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/16/2017] [Accepted: 08/17/2017] [Indexed: 12/16/2022] Open
Abstract
Radiation therapy induces DNA damage and inflammation leading to fibrosis. Fibrosis can occur 4 to 12 months after radiation therapy. This process worsens with time and years. Radiation-induced fibrosis is characterized by fibroblasts proliferation, myofibroblast differentiation, and synthesis of collagen, proteoglycans and extracellular matrix. Myofibroblasts are non-muscle cells that can contract and relax. Myofibroblasts evolve towards irreversible retraction during fibrosis process. In this review, we discussed the interplays between transforming growth factor-β1 (TGF-β1), canonical WNT/β-catenin pathway and peroxisome proliferator-activated receptor gamma (PPAR γ) in regulating the molecular mechanisms underlying the radiation-induced fibrosis, and the potential role of PPAR γ agonists. Overexpression of TGF-β and canonical WNT/β-catenin pathway stimulate fibroblasts accumulation and myofibroblast differentiation whereas PPAR γ expression decreases due to the opposite interplay of canonical WNT/β-catenin pathway. Both TGF-β1 and canonical WNT/β-catenin pathway stimulate each other through the Smad pathway and non-Smad pathways such as phosphatidylinositol 3-kinase/serine/threonine kinase (PI3K/Akt) signaling. WNT/β-catenin pathway and PPAR γ interact in an opposite manner. PPAR γ agonists decrease β-catenin levels through activation of inhibitors of the WNT pathway such as Smad7, glycogen synthase kinase-3 (GSK-3 β) and dickkopf-related protein 1 (DKK1). PPAR γ agonists also stimulate phosphatase and tensin homolog (PTEN) expression, which decreases both TGF-β1 and PI3K/Akt pathways. PPAR γ agonists by activating Smad7 decrease Smads pathway and then TGF-β signaling leading to decrease radiation-induced fibrosis. TGF-β1 and canonical WNT/β-catenin pathway promote radiation-induced fibrosis whereas PPAR γ agonists can prevent radiation-induced fibrosis.
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Affiliation(s)
- Alexandre Vallée
- Experimental and Clinical Neurosciences Laboratory, INSERM U1084, University of Poitiers, Poitiers, France.,Laboratory of Mathematics and Applications (LMA), UMR CNRS 7348, University of Poitiers, Poitiers, France
| | - Yves Lecarpentier
- Centre de Recherche Clinique, Grand Hôpital de l'Est Francilien (GHEF), Meaux, France
| | - Rémy Guillevin
- DACTIM, UMR CNRS 7348, University of Poitiers et CHU de Poitiers, Poitiers, France
| | - Jean-Noël Vallée
- Laboratory of Mathematics and Applications (LMA), UMR CNRS 7348, University of Poitiers, Poitiers, France.,CHU Amiens Picardie, University of Picardie Jules Verne (UPJV), Amiens, France
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36
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Komatsu D, Mistura DV, Motta A, Domingues JA, Hausen MA, Duek E. Development of a membrane of poly (L-co-D,L lactic acid-co-trimethylene carbonate) with aloe vera: An alternative biomaterial designed to improve skin healing. J Biomater Appl 2017; 32:311-320. [DOI: 10.1177/0885328217719854] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
Abstract
The search for new therapies and drugs that act as topical agents to relieve pain and control the infectious processes in burns always attracted interest in clinical trials. As an alternative to synthetic drugs, the use of natural extracts is useful in the development of new strategies and formulations for improving the life quality. The aim of this study was to develop a wound dressing using Poly(L-co-D,L lactic acid-co-TMC) (PLDLA-co-TMC) containing aloe vera (AV). This natural plant extract is known for its modulatory effects under healing process. The membrane of PLDLA-co-TMC+aloe vera was prepared at different concentrations of AV (5, 10, 15 and 50%). The FTIR showed no change in the PLDLA-co-TMC spectrum after AV addition, while the swelling test showed changes only in PLDLA-co-TMC+AV at 50%. The wettability measurements showed decrease in the contact angle in all samples after the AV addition in the polymer, while the AV release test showed that PLDLA-co-TMC+50%AV sample has higher AV release rate than the sample with other AV concentrations. The SEM analysis showed that AV was homogeneously distributed at 5% only. Tensile tests demonstrated an increase in the Young's modulus and a reduction in the elongation till rupture of the PLDLA-co-TMC after the addition of AV. Biocompatibility in vitro evaluation with fibroblast cells seeded in the membranes of PLDLA-co-TMC+AV showed that the cells were able to adhere, proliferate and maintain mitochondrial activity in all AV concentrations tested. Due to the known skin medicinal properties attributed to AV and the results here obtained, we suggest that after in vivo trials, the PLDLA-co-TMC+AV should be a promising biomaterial for application as a device for skin curative and healing agent.
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Affiliation(s)
- Daniel Komatsu
- Faculdade de Ciencias Medicas e da Saude, Pontificia Universidade Catolica de Sao Paulo, São Paulo, Brazil
| | - Daniel V Mistura
- Universidade Estadual de Campinas Instituto de Biologia, São Paulo, Brazil
| | - Adriana Motta
- Faculdade de Ciencias Medicas e da Saude, Pontificia Universidade Catolica de Sao Paulo, São Paulo, Brazil
| | | | - Moema A Hausen
- Faculdade de Ciencias Medicas e da Saude, Pontificia Universidade Catolica de Sao Paulo, São Paulo, Brazil
| | - Eliana Duek
- Faculdade de Ciencias Medicas e da Saude, Pontificia Universidade Catolica de Sao Paulo, São Paulo, Brazil
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Singaravelu S, Ramanathan G, Sivagnanam UT. Dual-layered 3D nanofibrous matrix incorporated with dual drugs and their synergetic effect on accelerating wound healing through growth factor regulation. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2017; 76:37-49. [DOI: 10.1016/j.msec.2017.02.148] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/17/2016] [Revised: 12/16/2016] [Accepted: 02/24/2017] [Indexed: 12/29/2022]
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38
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Rao S, Hegde SK, Baliga-Rao MP, Palatty PL, George T, Baliga MS. An Aloe Vera-Based Cosmeceutical Cream Delays and Mitigates Ionizing Radiation-Induced Dermatitis in Head and Neck Cancer Patients Undergoing Curative Radiotherapy: A Clinical Study. MEDICINES 2017; 4:medicines4030044. [PMID: 28930258 PMCID: PMC5622379 DOI: 10.3390/medicines4030044] [Citation(s) in RCA: 18] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 05/09/2017] [Revised: 06/14/2017] [Accepted: 06/19/2017] [Indexed: 11/16/2022]
Abstract
Background: This study was planned to evaluate the efficacy of topical application of an Aloe vera-based cream (AVC) for the prevention of ionizing radiation (X ray)-induced dermatitis in head and neck cancer patients requiring therapeutic radiation treatment (>62 Gy). Methods: From July 2012 to December 2012, a total of 60 head and neck cancer patients requiring curative radiotherapy (RT) of more than 66 Gy were prospectively enrolled and treated with AVC or a comparator Johnson’s Baby Oil (JBO). Acute skin reaction was monitored and classified according to the Radiation Therapy Oncology Group (RTOG) four-point rating scale on a weekly basis. Results: The results indicate that there was a statistically significant delay in the incidence (p = 0.04) of dermatitis at week three in the AVC application group. Application of AVC reduced the incidence of Grade 1, 2, and 3 dermatitis at subsequent time points, while Grade 4 dermatitis was not seen in either cohort. The other most important observation was that the continued application of AVC two weeks after the completion of RT was effective in reducing the average grade of dermatitis and was statistically significant (p < 0.02). Conclusions: Prophylactic use of an AVC-based cream is thus effective in delaying radiation dermatitis in head and neck cancer.
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Affiliation(s)
- Suresh Rao
- Department of Radiation Oncology, Mangalore Institute of Oncology, Mangalore 575002, India.
| | - Sanath Kumar Hegde
- Department of Radiation Oncology, Mangalore Institute of Oncology, Mangalore 575002, India.
| | | | - Princy Louis Palatty
- Department of Pharmacology, Father Muller Medical College, Kankanady, Mangalore 575002; India.
| | - Thomas George
- Department of Pharmacology, Father Muller Medical College, Kankanady, Mangalore 575002; India.
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Rahman S, Carter P, Bhattarai N. Aloe Vera for Tissue Engineering Applications. J Funct Biomater 2017; 8:E6. [PMID: 28216559 PMCID: PMC5371879 DOI: 10.3390/jfb8010006] [Citation(s) in RCA: 80] [Impact Index Per Article: 10.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/29/2016] [Revised: 02/05/2017] [Accepted: 02/07/2017] [Indexed: 01/15/2023] Open
Abstract
Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.
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Affiliation(s)
- Shekh Rahman
- Department of Chemical, Biological and Bioengineering, North Carolina A&T State University, Greensboro, NC 27411, USA.
| | - Princeton Carter
- Department of Chemical, Biological and Bioengineering, North Carolina A&T State University, Greensboro, NC 27411, USA.
| | - Narayan Bhattarai
- Department of Chemical, Biological and Bioengineering, North Carolina A&T State University, Greensboro, NC 27411, USA.
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Misawa E, Tanaka M, Saito M, Nabeshima K, Yao R, Yamauchi K, Abe F, Yamamoto Y, Furukawa F. Protective effects of Aloe sterols against UVB-induced photoaging in hairless mice. PHOTODERMATOLOGY PHOTOIMMUNOLOGY & PHOTOMEDICINE 2017; 33:101-111. [PMID: 27995657 DOI: 10.1111/phpp.12286] [Citation(s) in RCA: 28] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Accepted: 12/14/2016] [Indexed: 12/13/2022]
Abstract
BACKGROUND Aloe vera is a traditional medical plant whose gel has been widely used in skin care. Previously, we have identified Aloe sterols from Aloe vera as active ingredients. This study investigated the protective effects of Aloe sterols without polysaccharides, against ultraviolet B (UVB)-induced skin photoaging in mice using Aloe vera gel extract (AVGE) obtained by supercritical fluid extraction. METHODS Aloe vera gel extract was supplemented in the diet (12 or 120 ppm), and HR-1 hairless mice were exposed to UVB irradiation for 7 weeks. Skin measurements and histological and analytical studies were performed. RESULTS Repeated UVB irradiation induced rough wrinkling of skin with water content reduction and hyperkeratosis. AVGE administration resulted in the significant improvement of UVB-induced skin dryness, epidermal thickness, and wrinkle formation. The AVGE group also suppressed the degenerations of dermal collagen fibers and the appearance of cutaneous apoptosis cells induced by UVB. Furthermore, AVGE administration reduced the excess elevation of pro-inflammatory cytokines (IL-1β and TNF-α) and matrix metalloproteinases (MMP-2, MMP-9, MMP-12, and MMP-13) in UVB-exposed skin. CONCLUSION The dietary ingestion of Aloe sterols protected against chronic UVB damage in mouse skin, and our results suggest that Aloe sterols may prevent skin photoaging through the anti-inflammation and MMP regulation.
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Affiliation(s)
- Eriko Misawa
- Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd., Kanagawa, Japan
| | - Miyuki Tanaka
- Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd., Kanagawa, Japan
| | - Marie Saito
- Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd., Kanagawa, Japan
| | - Kazumi Nabeshima
- Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd., Kanagawa, Japan
| | - Ruiqing Yao
- Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd., Kanagawa, Japan
| | - Kouji Yamauchi
- Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd., Kanagawa, Japan
| | - Fumiaki Abe
- Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd., Kanagawa, Japan
| | - Yuki Yamamoto
- Department of Dermatology, Wakayama Medical University, Wakayama, Japan
| | - Fukumi Furukawa
- Department of Dermatology, Wakayama Medical University, Wakayama, Japan
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Ramanathan G, Singaravelu S, Muthukumar T, Thyagarajan S, Rathore HS, Sivagnanam UT, Perumal PT. Fabrication of Arothron stellatus skin collagen film incorporated with Coccinia grandis as a durable wound construct. INT J POLYM MATER PO 2017. [DOI: 10.1080/00914037.2016.1252351] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/14/2022]
Affiliation(s)
| | | | - Thangavelu Muthukumar
- Department of Physiology, College of Veterinary Medicine, Chonbuk National University, Gobong-ro, Iksan-city, Jeollabuk-Do, Republic of Korea
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Kalita S, Kandimalla R, Devi B, Kalita B, Kalita K, Deka M, Chandra Kataki A, Sharma A, Kotoky J. Dual delivery of chloramphenicol and essential oil by poly-ε-caprolactone–Pluronic nanocapsules to treat MRSA-Candida co-infected chronic burn wounds. RSC Adv 2017. [DOI: 10.1039/c6ra26561h] [Citation(s) in RCA: 24] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022] Open
Abstract
A novel feasible nano capsular approach through co-encapsulation of chloramphenicol and lemon-grass essential oil to combat chronic burn wound infections.
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Affiliation(s)
- Sanjeeb Kalita
- Drug Discovery Lab
- Institute of Advanced Study in Science and Technology
- Guwahati – 781035
- India
| | - Raghuram Kandimalla
- Drug Discovery Lab
- Institute of Advanced Study in Science and Technology
- Guwahati – 781035
- India
| | - Banasmita Devi
- Drug Discovery Lab
- Institute of Advanced Study in Science and Technology
- Guwahati – 781035
- India
| | - Bhupalee Kalita
- Jawaharlal Nehru Centre for Advanced Scientific Research
- Bengaluru
- India
| | | | - Manab Deka
- Department of Bioengineering and Technology
- Gauhati University
- Guwahati – 781014
- India
| | | | - Arup Sharma
- College of Veterinary Science
- Assam Agriculture University
- Guwahati – 781022
- India
| | - Jibon Kotoky
- Drug Discovery Lab
- Institute of Advanced Study in Science and Technology
- Guwahati – 781035
- India
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Topical Application of Aloe vera Accelerated Wound Healing, Modeling, and Remodeling. Ann Plast Surg 2016; 77:37-46. [DOI: 10.1097/sap.0000000000000239] [Citation(s) in RCA: 58] [Impact Index Per Article: 6.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2022]
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Balogun FO, Tshabalala NT, Ashafa AOT. Antidiabetic Medicinal Plants Used by the Basotho Tribe of Eastern Free State: A Review. J Diabetes Res 2016; 2016:4602820. [PMID: 27437404 PMCID: PMC4942634 DOI: 10.1155/2016/4602820] [Citation(s) in RCA: 21] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/31/2016] [Revised: 03/09/2016] [Accepted: 03/31/2016] [Indexed: 01/16/2023] Open
Abstract
Diabetes mellitus (DM) belongs to the group of five leading important diseases causing death globally and remains a major health problem in Africa. A number of factors such as poverty, poor eating habit, and hormonal imbalance are responsible for the occurrence of the disease. It poses a major health challenge in Africa continent today and the prevalence continues to increase at an alarming rate. Various treatment options particularly the usage of herbs have been effective against diabetes because they have no adverse effects. Interestingly, South Africa, especially the Basotho tribe, is blessed with numerous medicinal plants whose usage in the treatment of DM has been effective since the conventional drugs are expensive and often unaffordable. The present study attempted to update the various scientific evidence on the twenty-three (23) plants originating from different parts of the world but widely used by the Sotho people in the management of DM. Asteraceae topped the list of sixteen (16) plant families and remained the most investigated according to this review. Although limited information was obtained on the antidiabetic activities of these plants, it is however anticipated that government parastatals and scientific communities will pay more attention to these plants in future research.
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Affiliation(s)
- Fatai Oladunni Balogun
- Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, Qwaqwa Campus, Private Bag X 13, Phuthaditjhaba 9866, South Africa
| | - Natu Thomas Tshabalala
- Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, Qwaqwa Campus, Private Bag X 13, Phuthaditjhaba 9866, South Africa
| | - Anofi Omotayo Tom Ashafa
- Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, Qwaqwa Campus, Private Bag X 13, Phuthaditjhaba 9866, South Africa
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Anuradha A, Patil B, Asha VR. Evaluation of efficacy of aloe vera in the treatment of oral submucous fibrosis - a clinical study. J Oral Pathol Med 2016; 46:50-55. [PMID: 27314762 DOI: 10.1111/jop.12463] [Citation(s) in RCA: 32] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 04/27/2016] [Indexed: 12/22/2022]
Abstract
BACKGROUND Oral submucous fibrosis is a chronic disease, treatment of which has largely been symptomatic. Aloe vera has immunomodulatory, anti-inflammatory, wound healing, antioxidant, and antineoplastic activities. All such properties of aloe vera suggest the possibility of its use in the management of oral submucous fibrosis. METHODS Seventy-four patients of oral submucous fibrosis were randomly divided into 2 groups. Group A patients were treated with systemic (juice) and topical aloe vera (gel) for 3 months. Group B patients were treated with intralesional injection of hydrocortisone and hyaluronidase for 6 weeks with antioxidant supplements for 3 months. Patients were assessed for reduction in burning sensation and increase in mouth opening, cheek flexibility, and tongue protrusion at an interval of 1, 2, and 3 months. RESULTS Both the groups showed statistically significant improvements in all the study parameters at the end of study period (P < 0.001). The clinical response to aloe vera was comparable to that of intralesional injections of hydrocortisone and hyaluronidase with antioxidant supplementation. CONCLUSION The study concludes that aloe vera can be an alternative, safe, and effective treatment regime in the management of oral submucous fibrosis. Long-term follow-up studies with larger sample size are recommended.
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Affiliation(s)
- Ardra Anuradha
- Department of Oral medicine & radiology, The Oxford College and Hospital, Bangalore, India
| | - Bharati Patil
- Department of Oral medicine & radiology, The Oxford College and Hospital, Bangalore, India
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Singaravelu S, Ramanathan G, Muthukumar T, Raja MD, Nagiah N, Thyagarajan S, Aravinthan A, P G, Natarajan TS, V N Geetha Selva G, Kim JH, Sivagnanam UT. Durable keratin-based bilayered electrospun mats for wound closure. J Mater Chem B 2016; 4:3982-3997. [PMID: 32263097 DOI: 10.1039/c6tb00720a] [Citation(s) in RCA: 26] [Impact Index Per Article: 2.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
Abstract
A bilayered nanofibrous scaffold with rapid wound healing properties is found to be suitable for tissue regeneration applications. The objective of this study is to reveal the fabrication of a poly(3-hydroxybutyric acid) (P)-gelatin (G) nanofibrous mat through electrospinning, with a horn keratin-chitosan-based biosheet (KC) as a bilayered nanofibrous scaffold. The mupirocin (D)-loaded horn KC biosheet (KCD) acts as the primary layer over which PG nanofibers were electrospun to act as the secondary layer. It is shown that this engineered bilayered nanofibrous scaffold material (KC-PG) should fulfill the functions of the extracellular matrix (ECM) by elucidating its function in vitro and in vivo. The bilayered nanofibrous scaffold was designed to exhibit improved physiochemical, biological and mechanical properties, with better swelling and porosity for enhanced oxygen permeability, and it also exhibits an acceptable antibacterial property to prevent infection at the wound site. The bilayered nanofibrous scaffold assists in better biocompatibility towards fibroblast and keratinocyte cell lines. The morphology of the nanofibrous scaffold aids increased cell adhesion and proliferation with cell material interactions. This was elucidated with the help of in vitro fluorescence staining against both cell lines. The bilayered KCD-PG nanofibrous scaffold material gives accelerated wound healing efficiency during in vivo wound healing. The results showed the regulation of growth factors with enhanced collagen synthesis, thereby helping in faster wound healing.
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Affiliation(s)
- Sivakumar Singaravelu
- Bioproducts Lab, CSIR-Central Leather Research Institute, Chennai 600020, Tamilnadu, India.
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Latif MA, Zaki MZM, Leng TM, Rahman NHA, Arshad SA, Hamid A. Alocasia denudata Engler treatment enhance open wound healing activities in Wistar rat's skin. JOURNAL OF ETHNOPHARMACOLOGY 2015; 176:258-267. [PMID: 26519202 DOI: 10.1016/j.jep.2015.10.036] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/27/2015] [Revised: 09/21/2015] [Accepted: 10/24/2015] [Indexed: 06/05/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE A. denudata is traditionally used to treat various skin disorders, including wounds. It is widely used by the traditional healers as an effective wound treatment. AIM OF STUDY This study was done to determine A. denudata treatment effects on open wound healing activities in Wistar rat's skin. MATERIALS AND METHODS 120 Wistar rats (250-300 g) were divided into four main groups, 1.5% and 3% A. denudata stem juice treated group, 10% Solcoseryl® gel treated group as positive control and phosphate buffer saline (PBS) treated group as negative control. Six full thicknesses wounds (6mm) were induced bilaterally on the dorsal of the rat's skin. Rats were sacrificed on day 1, 3, 6, 10 and 14. The percentage of wound contraction, biochemical estimations, macroscopic observation and histological examinations were done to evaluate the wound healing activities. RESULTS Results showed wounds treated with A. denudata stem juice possess a significant higher rate of wound contraction (p<0.001), total protein concentration (p<0.05), hexosamine concentration (p<0.001) and uronic acid concentration (p<0.001). Moreover, cathepsin B (p<0.05) and hydroxyproline (p<0.05) level showed lower concentration in wounds treated with A. denudata stem juice. Histological observation of wounds treated with A. denudata stem juice displayed organized epithelial layer with dense and compact collagen fibers. CONCLUSION Both doses of A. denudata stem juice were found to enhance wound healing process. However, wounds treated with 3% A. denudata stem juice were reported to be more effective as a wound healing agent thus support its traditional usage.
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Affiliation(s)
- Mazlyzam Abdul Latif
- Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
| | - Mohd Zulasyraf Mohd Zaki
- Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
| | - Tai May Leng
- Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
| | - Noor Hidayah Abdul Rahman
- Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
| | - Siti Aisyah Arshad
- Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
| | - Asmah Hamid
- Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
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Ammar I, Bardaa S, Mzid M, Sahnoun Z, Rebaii T, Attia H, Ennouri M. Antioxidant, antibacterial and in vivo dermal wound healing effects of Opuntia flower extracts. Int J Biol Macromol 2015; 81:483-90. [DOI: 10.1016/j.ijbiomac.2015.08.039] [Citation(s) in RCA: 33] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/23/2015] [Revised: 08/09/2015] [Accepted: 08/20/2015] [Indexed: 10/23/2022]
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Murali R, Ponrasu T, Cheirmadurai K, Thanikaivelan P. Biomimetic hybrid porous scaffolds immobilized with platelet derived growth factor-BB promote cellularization and vascularization in tissue engineering. J Biomed Mater Res A 2015; 104:388-96. [DOI: 10.1002/jbm.a.35574] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/30/2015] [Revised: 09/04/2015] [Accepted: 09/23/2015] [Indexed: 11/06/2022]
Affiliation(s)
- Ragothaman Murali
- Advanced Materials Laboratory, Centre for Leather Apparel & Accessories Development; Central Leather Research Institute (Council of Scientific and Industrial Research); Adyar Chennai 600020 India
| | - Thangavel Ponrasu
- Department of Biochemistry; Central Leather Research Institute (Council of Scientific and Industrial Research); Adyar Chennai 600020 India
| | - Kalirajan Cheirmadurai
- Advanced Materials Laboratory, Centre for Leather Apparel & Accessories Development; Central Leather Research Institute (Council of Scientific and Industrial Research); Adyar Chennai 600020 India
| | - Palanisamy Thanikaivelan
- Advanced Materials Laboratory, Centre for Leather Apparel & Accessories Development; Central Leather Research Institute (Council of Scientific and Industrial Research); Adyar Chennai 600020 India
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